ATP-dependent 6-phosphofructokinase

Overview


Name	ATP-dependent 6-phosphofructokinase
Smed ID	SMED30026981
Length (bp)	2390

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of ATP-dependent 6-phosphofructokinase (SMED30026981) t-SNE clustered cells

Violin plots show distribution of expression levels for ATP-dependent 6-phosphofructokinase (SMED30026981) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30026981

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
reproductive organ	SMED30026981	SMESG000074512.1	Contig54429	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30026981	SMESG000074512.1	Contig54429	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30026981	SMESG000074570.1 SMESG000074512.1 SMESG000023258.1	Contig17553	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30026981	SMESG000074570.1 SMESG000074512.1 SMESG000023258.1	Contig17553	newmark_ests	PMID:28434803 Rouhana et al., 2017
testis primordium	SMED30026981	SMESG000074512.1	PL06023B1A08_SE3	GPL10652	PMID:20844018 Wang et al., 2010

Note: Hover over icons to view figure legend

Homology

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Human
Match: PFKP (phosphofructokinase, platelet [Source:HGNC Symbol;Acc:HGNC:8878])

HSP 1 Score: 488.419 bits (1256), Expect = 0.000e+0
Identity = 262/519 (50.48%), Postives = 352/519 (67.82%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            KF+      G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QG++DGG NI EA W  VS ++ +GGT+IGSARC  F+TR GRLKAA NL+ R I NL +IGGDGSLTGAN+F++EW+  ++EL     ID    +    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP + WE+ +C KL E ++  +R+N++I++EGAID  N+PIT++ IK++++ ++  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N  VR PLM+CV  T D+Q  M    F D V +RG SF  N   Y+ L + +P          +I K            T+  + ++  G  A GMNA +RS VR+ IA+    + I  GFDG  K  I  + W DV G    GGSI+GT RVLP +   E +A+++    ++AL+IIGGFE
Sbjct:   15 KFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEA--TPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDD--------QIPK------------TNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKY-LEEIATQMRTHSINALLIIGGFE 510

HSP 2 Score: 206.068 bits (523), Expect = 0.000e+0
Identity = 105/258 (40.70%), Postives = 162/258 (62.79%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY--------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   ++K++ F +P+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+    A+ W+   L+           +DSI +LG+S+    NVI  P+    +  D+E   P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  517 ELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKR---NVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVS 771

HSP 3 Score: 112.464 bits (280), Expect = 8.761e-25
Identity = 98/353 (27.76%), Postives = 168/353 (47.59%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA---IHALIDAAQ--------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++AV+  G  + GMNAAV+S VR+G+  G  +  + +G  G   G   I+E  W+DV G    GG+++G+ R L  K      + A  +    I  L+IIGG  +  G              L+E        +++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +        + L E   +  +  LV+ +E   + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A A   I A +  A+        + SI  CV+ I K  ++ +P+ +   +T
Sbjct:  412 NVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKG--QIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLE---EIATQMRTHSINALLIIGGFEAYLG--------------LLELSAAREKHEEFC--VPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSI--CVLGISKRNVIFQPVAELKKQT 739

HSP 4 Score: 66.2402 bits (160), Expect = 1.355e-15
Identity = 48/169 (28.40%), Postives = 83/169 (49.11%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKL-FKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            VV +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  ++ E         Q    L      + +  +++      +  + P  S  I +L   Q G   +D R   +GHV  GG  S+FDR L +R G+EA+I
Sbjct:  167 VVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLG---YDTRVTILGHVQRGGTPSAFDRILASRMGVEAVI 331

HSP 5 Score: 37.7354 bits (86), Expect = 1.355e-15
Identity = 43/127 (33.86%), Postives = 61/127 (48.03%), Query Frame = 2

Query: 1247 MVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            M  + +R  K S RK     S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I    W  V  I+ +GG+IIG+ R     ++ G    A  LL+  ++ L +IGG
Sbjct:    1 MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGG 127

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Human
Match: PFKM (phosphofructokinase, muscle [Source:HGNC Symbol;Acc:HGNC:8877])

HSP 1 Score: 472.241 bits (1214), Expect = 0.000e+0
Identity = 258/529 (48.77%), Postives = 351/529 (66.35%), Query Frame = 2

Query:   35 EEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            EE+++  +E       G  G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +I+EA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  + +L +   I   +      LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P           +SK                + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +  FE +++ + +F +  L+IIGGFE
Sbjct:  100 EEWIMTHEEHHAAKTLGI-GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPP----------VSKSGSHT-----------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-SFEQISANITKFNIQGLVIIGGFE 603

HSP 2 Score: 197.978 bits (502), Expect = 0.000e+0
Identity = 106/259 (40.93%), Postives = 156/259 (60.23%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F+VG+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +  +    P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  608 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRK-RALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 865

HSP 3 Score: 126.716 bits (317), Expect = 3.424e-29
Identity = 110/362 (30.39%), Postives = 185/362 (51.10%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            P      H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K+     + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D ++G ++AL  I    D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  497 PVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQT 833

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Human
Match: PFKM (phosphofructokinase, muscle [Source:HGNC Symbol;Acc:HGNC:8877])

HSP 1 Score: 470.7 bits (1210), Expect = 0.000e+0
Identity = 254/510 (49.80%), Postives = 343/510 (67.25%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +I+EA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  + +L +   I   +      LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P           +SK                + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +  FE +++ + +F +  L+IIGGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPP----------VSKSGSHT-----------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-SFEQISANITKFNIQGLVIIGGFE 500

HSP 2 Score: 197.978 bits (502), Expect = 0.000e+0
Identity = 106/259 (40.93%), Postives = 155/259 (59.85%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F+VG+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +       P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  505 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALV-FQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 762

HSP 3 Score: 126.716 bits (317), Expect = 2.658e-29
Identity = 110/362 (30.39%), Postives = 185/362 (51.10%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            P      H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K+     + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D ++G ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  394 PVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQT 730

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Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +I+EA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  + +L +   I   +      LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P           +SK                + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +  FE +++ + +F +  L+IIGGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPP----------VSKSGSHT-----------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-SFEQISANITKFNIQGLVIIGGFE 500

HSP 2 Score: 197.978 bits (502), Expect = 0.000e+0
Identity = 106/259 (40.93%), Postives = 155/259 (59.85%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F+VG+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +       P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  505 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALV-FQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 762

HSP 3 Score: 126.716 bits (317), Expect = 2.658e-29
Identity = 110/362 (30.39%), Postives = 185/362 (51.10%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            P      H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K+     + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D ++G ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  394 PVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQT 730

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Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +I+EA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  + +L +   I   +      LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P           +SK                + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +  FE +++ + +F +  L+IIGGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPP----------VSKSGSHT-----------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-SFEQISANITKFNIQGLVIIGGFE 500

HSP 2 Score: 197.978 bits (502), Expect = 0.000e+0
Identity = 106/259 (40.93%), Postives = 155/259 (59.85%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F+VG+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +       P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  505 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALV-FQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 762

HSP 3 Score: 126.716 bits (317), Expect = 2.658e-29
Identity = 110/362 (30.39%), Postives = 185/362 (51.10%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            P      H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K+     + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D ++G ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  394 PVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQT 730

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Celegans
Match: pfk-1.1 (ATP-dependent 6-phosphofructokinase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TZL8])

HSP 1 Score: 462.996 bits (1190), Expect = 3.307e-180
Identity = 244/522 (46.74%), Postives = 343/522 (65.71%), Query Frame = 2

Query:   56 KEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +EG  I +  F+G ++A+ TSGGDSQGMN+AV+S+ RM +YCG  VY + EG +G+I+GG  I+EA W+ VS ++  GGT+IGSAR  +F+TR GRLKAA NLI+R I  LV IGGDGSLTGAN F+ EWT  + EL++ + + +   K    + IVGLVGSIDNDFCGTDMTIG +SAL RI+ + DA++ TA SHQR FVIEVMGRHCGYLAL+ A++SEA F FIPE P  +NW D LC KL + +S  +R+N++I++EGAID   + ITA+ +K  + +++K DTR+T+LGHVQRGG P++FDR+L  R GAEA+ AL++  +      CVI I  N +VR PL+ CV +T  +Q  M +  +   VM+RG SF RN + Y+ L  +    +  + EG + +                 + ++  G  A GMNA +RS VRMA+ +     GI+  F+GL   +     W+DV      GGS +GT + LP++     +A++L +  + AL+++GGFE
Sbjct:   33 REGTEIASDIFKGRTLAIYTSGGDSQGMNSAVRSITRMAIYCGCKVYLIYEGYEGMIEGGDFIKEATWNTVSDIIQQGGTIIGSARSSEFRTREGRLKAATNLINRGIGRLVCIGGDGSLTGANTFRLEWTDLVQELVKNQRVTAAAAKKIPYIQIVGLVGSIDNDFCGTDMTIGTDSALQRIISSIDAVVATAQSHQRAFVIEVMGRHCGYLALVAALASEADFCFIPEWPAPENWRDVLCDKLSQMRSEGQRLNIIIVAEGAIDRDGKAITAEDVKTAVKEKLKYDTRVTILGHVQRGGAPSAFDRLLGCRMGAEAVFALMEMTEESE--PCVISIDGNVMVRVPLLKCVERTQMVQKAMADKDWTTAVMLRGRSFQRNLETYKLLTKMRTVEKDNLSEGHKFN-----------------VAVINVGAPAGGMNAAVRSYVRMALYHQCTVYGIEDSFEGLANGSFKQFKWSDVTNWAMNGGSFLGTQKSLPTEKTMPQLAAQLKKHNIQALLLVGGFE 535

HSP 2 Score: 189.889 bits (481), Expect = 3.307e-180
Identity = 101/263 (38.40%), Postives = 152/263 (57.79%), Query Frame = 3

Query: 1632 LWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDNDSIVI---------LGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIMRHL 2390
            L +N++KY  F IP+ +IP TI+NN+PG   ++GSDT +N I   +D +K  + GT+ R++I ET+G  CGYL T+ AL+ GAD +YI EE   ++ L+ DV+++ +K+    K  LV+RN  +     + ++  LF  EGK  F  R N +GHV  GG  + FDRN+GT+    A+ ++   L EN   D+ VI         LGL +       P+    +  D+E   P +QWWM LRPLLR++  H S  E+S I+  +
Sbjct:  543 LAENREKYPEFCIPMCVIPCTISNNVPGTMVSLGSDTAINEICQMIDKIKQSATGTKRRVFIVETMGGYCGYLATLSALSSGADNAYIFEEPFTVQDLSDDVDVILSKMEVGAKRYLVVRNEWADKNLTTDFVQNLFDSEGKKNFTTRVNVLGHVQQGGSPTPFDRNMGTKLAARALEFLLIQLKENLTADNKVIAKSAHTATLLGL-KGRKVVFTPVQDLKKETDFEHRLPSEQWWMALRPLLRVLARHRSTVESSAILESV 804

HSP 3 Score: 120.168 bits (300), Expect = 1.036e-27
Identity = 100/347 (28.82%), Postives = 177/347 (51.01%), Query Frame = 2

Query:    2 MYKEFSLQTDNEEFLLKSKEGKFIPAGGFQGH--SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXX--XXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALI 1024
            M +  S Q + E + L +K          +GH  ++AV+  G  + GMNAAV+S VRM LY   TVY + +  +GL +G  + ++  WSDV+     GG+ +G+ + L   T     + A  L    I  L+++GG  +     I  +    Y               ++C  + ++    +I N+  GT +++G ++A+  I +  D +  +A  + +R F++E MG +CGYLA ++A+SS A   +I E+P    +  DD+   L + +   +R  LV+ +E A    ++ +T  +++++     +    TR+ VLGHVQ+GG+PT FDR +  +  A A+  L+
Sbjct:  402 MLRGRSFQRNLETYKLLTKMRTVEKDNLSEGHKFNVAVINVGAPAGGMNAAVRSYVRMALYHQCTVYGIEDSFEGLANG--SFKQFKWSDVTNWAMNGGSFLGTQKSL--PTEKTMPQLAAQLKKHNIQALLLVGGFEAYHSTIILAENREKY--------------PEFCIPMCVIPC--TISNNVPGTMVSLGSDTAINEICQMIDKIKQSATGTKRRVFIVETMGGYCGYLATLSALSSGADNAYIFEEPFTVQDLSDDVDVILSKMEVGAKRY-LVVRNEWA----DKNLTTDFVQNLFDSEGKKNFTTRVNVLGHVQQGGSPTPFDRNMGTKLAARALEFLL 723

HSP 4 Score: 75.8702 bits (185), Expect = 8.569e-14
Identity = 60/216 (27.78%), Postives = 99/216 (45.83%), Query Frame = 3

Query: 1626 QELWKNKD-----KYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILG 2219
            QEL KN+         I  I +V +  +I N+  G    +G+D+ +  I++++D + + +  +  R ++ E +G +CGYL  + ALA  AD  +I E             ++  + S  Q +NI+       +  G + R+  +    D     K      K  +D R   +GHV  GG  S+FDR LG R G EA+  + +M E  +   I I G
Sbjct:  167 QELVKNQRVTAAAAKKIPYIQIVGLVGSIDNDFCGTDMTIGTDSALQRIISSIDAV-VATAQSHQRAFVIEVMGRHCGYLALVAALASEADFCFIPEWPAPENWRDVLCDKLSQMRSEGQRLNIII------VAEGAIDRDGKAITAEDVKTAVK-----EKLKYDTRVTILGHVQRGGAPSAFDRLLGCRMGAEAVFALMEMTEESEPCVISIDG 370

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Celegans
Match: pfk-1.2 (ATP-dependent 6-phosphofructokinase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q27483])

HSP 1 Score: 328.176 bits (840), Expect = 3.701e-121
Identity = 208/524 (39.69%), Postives = 303/524 (57.82%), Query Frame = 2

Query:   95 HSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELI-ERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPS--------IPACVICIVKNEIVRKPLMDCVSKTLDI-QNLMRNGIFVDLV-MMRGPSF-----YRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGF 1618
            H + VLTSGGDSQGMNAAV+SVVR  +  G   Y + EG  GLI+G  NI  A W+ V+ + +LGG++IG++RC +F+T  GR KAA  + D++IF+L++IGGDGSL GA   K+EW  + +EL  E K+ + +  +  + L++ G+VGSIDND   +D +IG ++AL RI EA D L+ TA SHQR FV+EVMGRHCGYLAL  AI+ EA +VF PE PP++ W + LC +L   +   +R N++I+ EG  +   Q I  + +K+ I  R++++ RI  LGH+QRGG P+  DR++ LR G EA+  ++   +             A V+C+  + I R  L   + +T    + +M+     DL   +RG  F     Y N+ +   L+  MP   K                    +     IG  C+     GMNA   S  RMA  + +  IGIK+G+DGL  K +  L W +V+G    GGS++GT R LPS++  +++A  L    +  L+IIGGF
Sbjct:   11 HGVGVLTSGGDSQGMNAAVRSVVRETIRQGHRCYLIREGYNGLING--NIELAKWAHVANVTHLGGSMIGTSRCDEFRTTDGRKKAAAIMFDKRIFHLIVIGGDGSLMGAQKLKEEWGRFGEELFAEGKITEEVANE-GRELHLAGIVGSIDNDCIESDKSIGSDTALHRICEAIDGLVMTAQSHQRVFVVEVMGRHCGYLALTAAIAVEADYVFYPEIPPDEKWPEQLCHQLGSVRKMGKRQNVIILGEGVTNSKGQRIDVRQVKEEIETRLQLEVRIATLGHLQRGGAPSFLDRLIGLRMGYEAVQEVLKGKEEKEGAVVTGQKTIAKVMCLRGHNIQRNELSRVIRQTETANEEIMQR--HSDLACRLRGFGFLDKQTYLNF-VSIPLSTTMPSRTK--------------------TFAVVHIGSPCA-----GMNAATYSFTRMANHSGIQVIGIKHGWDGLKNKDVKLLTWANVQGWAQFGGSMLGTKRQLPSEM--DLIAEGLNSNNVDGLVIIGGF 501

HSP 2 Score: 127.872 bits (320), Expect = 3.701e-121
Identity = 74/242 (30.58%), Postives = 131/242 (54.13%), Query Frame = 3

Query: 1632 LWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYG-----LVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRII 2342
            L +N+ +Y    IP+V+IPATI+NN PG   ++G DT +N I   +DN+   +IG++ ++ I ET+G+  G+L TM AL+ G+  + I +    +E+  +D+  L  +  + +  G     L++R+  +     S  + K+F +  K  +  R  ++G+  LGG  S FDR +G R G+ A   + + ++  D D  VI GL + +S   +P+    +   +E   P + WW+ L PL+  +
Sbjct:  510 LQQNRSEYTCLSIPIVVIPATISNNCPGTCMSLGVDTALNEICRQVDNISQNAIGSKNKVMIIETMGSRSGFLATMTALSTGSQFALIRQ----VETNEKDLEKLAIETKERLDSGNLEKFLLIRSEGASDEIYSPDVKKIFDKVMKNKYGVRITNLGYSQLGGHPSCFDRQMGIRMGVRAFEGIVNPVKMGDRDCCVI-GL-RGSSLRYVPVQGLGKKVCFEHGVPHNMWWLDLHPLVEAM 745

HSP 3 Score: 113.62 bits (283), Expect = 1.090e-25
Identity = 102/361 (28.25%), Postives = 163/361 (45.15%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAK----SSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDL 1165
            + AV+  G    GMNAA  S  RM  + G  V  +  G  GL +   +++   W++V G    GG+++G+ R L  +        A+ L    +  LVIIGG  +   A I +Q  + Y                 C ++ IV +  +I N+  GT M++GV++AL  I    D +   A  S  +  +IE MG   G+LA +TA+S+ +QF  I +   N   E DL K   E K    S N    L+I SEGA D    P   +    ++ ++  +      LG+ Q GG+P+ FDR + +R G  A   +++  +      CVI +  + +   P+     K      +  N  ++DL
Sbjct:  405 TFAVVHIGSPCAGMNAATYSFTRMANHSGIQVIGIKHGWDGLKNK--DVKLLTWANVQGWAQFGGSMLGTKRQLPSEMDL----IAEGLNSNNVDGLVIIGGFMAFESALILQQNRSEYT----------------CLSIPIVVIPATISNNCPGTCMSLGVDTALNEICRQVDNISQNAIGSKNKVMIIETMGSRSGFLATMTALSTGSQFALIRQVETN---EKDLEKLAIETKERLDSGNLEKFLLIRSEGASDEIYSPDVKKIFDKVMKNKYGVRITN--LGYSQLGGHPSCFDRQMGIRMGVRAFEGIVNPVKMGDRDCCVIGLRGSSLRYVPVQGLGKKVCFEHGVPHNMWWLDL 738

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Match: Pfk (gene:FBgn0003071 transcript:FBtr0088421)

HSP 1 Score: 496.508 bits (1277), Expect = 0.000e+0
Identity = 269/519 (51.83%), Postives = 361/519 (69.56%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +F+  G  +   +AV TSGGDSQGMNAAV++ VRM +Y G  VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYLA+  AI++EA F+FIPE+P + NW+D++C KL + +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:    8 RFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLAISAAIATEADFMFIPEEPVSVNWKDEICVKLHQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 509

HSP 2 Score: 169.088 bits (427), Expect = 0.000e+0
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  516 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 770

HSP 3 Score: 134.035 bits (336), Expect = 5.575e-32
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  381 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 740

HSP 4 Score: 71.2478 bits (173), Expect = 2.847e-12
Identity = 69/262 (26.34%), Postives = 118/262 (45.04%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLR--------NNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL    A+A  AD  +I EE +++   ++    +  KL  E   G  L          +   HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  140 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLAISAAIATEADFMFIPEEPVSVNWKDE----ICVKLHQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 388

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Match: Pfk (gene:FBgn0003071 transcript:FBtr0088422)

HSP 1 Score: 494.967 bits (1273), Expect = 0.000e+0
Identity = 268/519 (51.64%), Postives = 357/519 (68.79%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +F+  G  +   +AV TSGGDSQGMNAAV++ VRM +Y G  VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYLAL+  ++ EA F+FIPE PP  +W D LC +L + +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:    8 RFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 509

HSP 2 Score: 169.088 bits (427), Expect = 0.000e+0
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  516 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 770

HSP 3 Score: 134.42 bits (337), Expect = 4.514e-32
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  381 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 740

HSP 4 Score: 70.0922 bits (170), Expect = 6.003e-12
Identity = 70/267 (26.22%), Postives = 118/267 (44.19%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL  +G LA  AD  +I E             ++    S  Q +NI+       +  G + R     HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  140 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVI------VAEGAMDREG---HPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 388

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Match: Pfk (gene:FBgn0003071 transcript:FBtr0088420)

HSP 1 Score: 493.812 bits (1270), Expect = 0.000e+0
Identity = 268/516 (51.94%), Postives = 355/516 (68.80%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            P   F+   +AV TSGGDSQGMNAAV++ VRM +Y G  VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYLAL+  ++ EA F+FIPE PP  +W D LC +L + +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:  173 PVKPFKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 671

HSP 2 Score: 168.703 bits (426), Expect = 0.000e+0
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  678 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 932

HSP 3 Score: 134.42 bits (337), Expect = 6.827e-32
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  543 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 902

HSP 4 Score: 69.707 bits (169), Expect = 7.287e-12
Identity = 70/267 (26.22%), Postives = 118/267 (44.19%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL  +G LA  AD  +I E             ++    S  Q +NI+       +  G + R     HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  302 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVI------VAEGAMDREG---HPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 550

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Match: Pfk (gene:FBgn0003071 transcript:FBtr0304925)

HSP 1 Score: 491.115 bits (1263), Expect = 0.000e+0
Identity = 268/516 (51.94%), Postives = 355/516 (68.80%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            P   F+   +AV TSGGDSQGMNAAV++ VRM +Y G  VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYL+++  I SEA +VF+PE PP  +W D L  KLE+ +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:  173 PVKPFKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 671

HSP 2 Score: 169.088 bits (427), Expect = 0.000e+0
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  678 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 932

HSP 3 Score: 134.035 bits (336), Expect = 8.059e-32
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  543 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 902

HSP 4 Score: 67.781 bits (164), Expect = 3.195e-11
Identity = 70/267 (26.22%), Postives = 119/267 (44.57%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEE----------VLNLE---SLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL+ +  +   AD  ++ E           VL LE   S  Q +NI+       +  G + R     HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  302 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVI------VAEGAMDREG---HPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 550

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Match: Pfk (gene:FBgn0003071 transcript:FBtr0333067)

HSP 1 Score: 446.817 bits (1148), Expect = 6.288e-169
Identity = 246/477 (51.57%), Postives = 328/477 (68.76%), Query Frame = 2

Query:  191 VYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYL+++  I SEA +VF+PE PP  +W D L  KLE+ +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:  184 VYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 643

HSP 2 Score: 168.703 bits (426), Expect = 6.288e-169
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  650 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 904

HSP 3 Score: 134.42 bits (337), Expect = 5.897e-32
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  515 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 874

HSP 4 Score: 67.781 bits (164), Expect = 3.408e-11
Identity = 70/267 (26.22%), Postives = 119/267 (44.57%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEE----------VLNLE---SLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL+ +  +   AD  ++ E           VL LE   S  Q +NI+       +  G + R     HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  274 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVI------VAEGAMDREG---HPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 522

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Match: pfkpa (phosphofructokinase, platelet a [Source:ZFIN;Acc:ZDB-GENE-091112-22])

HSP 1 Score: 491.5 bits (1264), Expect = 0.000e+0
Identity = 259/510 (50.78%), Postives = 355/510 (69.61%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVRMG+Y GA VYFV EG QG++DGG NI+EA W +VS M+ +GGTVIGSARC DF+T  GRLKAA NLI R I NL +IGGDGSLTGAN+F++EW+  + EL+++ +ID    +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL   ++  +R+N++I++EGAID  NQPIT++++K++++ R+  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++   N   PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N K Y+ L      HRK   E             L +S  +  + ++  G  A GMNA +RS VR+ I+       +  GF+G  K  I  + W DV G    GGS++GT R LP++   + +A ++ ++ ++AL++IGGFE
Sbjct:   17 GKAIAVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFVHEGYQGMVDGGDNIKEATWENVSSMLQVGGTVIGSARCKDFRTHEGRLKAAHNLIQRGITNLCVIGGDGSLTGANLFREEWSGLLAELVQQGLIDEDAAQKYSALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSAKRAGMKRLNIIIVAEGAIDRKNQPITSEHVKNLVVSRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLETTAN--TPACVVSLCGNQSVRLPLMECVQMTQEVQKAMDGKRFEEAVQLRGRSFENNLKTYKLL-----AHRKPESE-------------LPHS--NFNVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKGQIKEIKWADVGGWTGQGGSLLGTKRTLPAK-HIDKIAEQMRKYNINALLVIGGFE 503

HSP 2 Score: 192.971 bits (489), Expect = 0.000e+0
Identity = 100/257 (38.91%), Postives = 158/257 (61.48%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL + +  Y+   +P+V++PAT++NN+PG   ++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E+Y          DS  +LG+ +       P+    +  D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  510 ELLEARRTYEELCVPMVMVPATVSNNVPGSDLSIGADTALNAITATCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMV-FQPVVQLKDETDFVHRIPKEQWWLKLRPLMKILAKYKTSYDVS 765

HSP 3 Score: 118.627 bits (296), Expect = 5.601e-27
Identity = 104/383 (27.15%), Postives = 185/383 (48.30%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            + F       + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  ++ V +G +G   G   I+E  W+DV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +  G           I EL+E +      ++ C  + +V +  ++ N+  G+D++IG ++AL  I    D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  +               N    AC++ + +  +V +P++    +T
Sbjct:  378 RSFENNLKTYKLLAHRKPESELPHSNF---NVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKG--QIKEIKWADVGGWTGQGGSLLGTKRTLPAKHID---KIAEQMRKYNINALLVIGGFEAYLG-----------IMELLEAR---RTYEELC--VPMVMVPATVSNNVPGSDLSIGADTALNAITATCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQR-GLVLRNENSNENYTTDFI--YQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMVFQPVVQLKDET 733

HSP 4 Score: 70.8626 bits (172), Expect = 4.602e-12
Identity = 55/195 (28.21%), Postives = 95/195 (48.72%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE---EVLNLESLNQDVNILKAKLFDE----IKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTS 2237
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD   I E   E    E + Q ++  +A +       +  G + R N    P  S ++  L     +  FD R   +GHV  GG  S+FDR L +R G+EA++    +LE   N    ++ L  + S
Sbjct:  160 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSAKRAGMKRLNIIIVAEGAIDRKN---QPITSEHVKNLVVS--RLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLETTANTPACVVSLCGNQS 345

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Match: pfkpa (phosphofructokinase, platelet a [Source:ZFIN;Acc:ZDB-GENE-091112-22])

HSP 1 Score: 484.952 bits (1247), Expect = 0.000e+0
Identity = 259/510 (50.78%), Postives = 351/510 (68.82%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVRMG+Y GA VYFV EG QG++DGG NI+EA W +VS M+ +GGTVIGSARC DF+T  GRLKAA NLI R I NL +IGGDGSLTGAN+F++EW+  + EL+++ +ID    +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL   +S   R+N++I++EGAID H + IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++   N   PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N K Y+ L      HRK   E             L +S  +  + ++  G  A GMNA +RS VR+ I+       +  GF+G  K  I  + W DV G    GGS++GT R LP++   + +A ++ ++ ++AL++IGGFE
Sbjct:   17 GKAIAVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFVHEGYQGMVDGGDNIKEATWENVSSMLQVGGTVIGSARCKDFRTHEGRLKAAHNLIQRGITNLCVIGGDGSLTGANLFREEWSGLLAELVQQGLIDEDAAQKYSALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSATRSRGSRLNIIIVAEGAIDRHGKAITSSIVKDLVVSRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLETTAN--TPACVVSLCGNQSVRLPLMECVQMTQEVQKAMDGKRFEEAVQLRGRSFENNLKTYKLL-----AHRKPESE-------------LPHS--NFNVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKGQIKEIKWADVGGWTGQGGSLLGTKRTLPAK-HIDKIAEQMRKYNINALLVIGGFE 503

HSP 2 Score: 192.971 bits (489), Expect = 0.000e+0
Identity = 100/257 (38.91%), Postives = 158/257 (61.48%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL + +  Y+   +P+V++PAT++NN+PG   ++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E+Y          DS  +LG+ +       P+    +  D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  510 ELLEARRTYEELCVPMVMVPATVSNNVPGSDLSIGADTALNAITATCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMV-FQPVVQLKDETDFVHRIPKEQWWLKLRPLMKILAKYKTSYDVS 765

HSP 3 Score: 118.242 bits (295), Expect = 7.534e-27
Identity = 104/383 (27.15%), Postives = 185/383 (48.30%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            + F       + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  ++ V +G +G   G   I+E  W+DV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +  G           I EL+E +      ++ C  + +V +  ++ N+  G+D++IG ++AL  I    D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  +               N    AC++ + +  +V +P++    +T
Sbjct:  378 RSFENNLKTYKLLAHRKPESELPHSNF---NVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKG--QIKEIKWADVGGWTGQGGSLLGTKRTLPAKHID---KIAEQMRKYNINALLVIGGFEAYLG-----------IMELLEAR---RTYEELC--VPMVMVPATVSNNVPGSDLSIGADTALNAITATCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQR-GLVLRNENSNENYTTDFI--YQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMVFQPVVQLKDET 733

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Match: pfkpa (phosphofructokinase, platelet a [Source:ZFIN;Acc:ZDB-GENE-091112-22])

HSP 1 Score: 484.567 bits (1246), Expect = 0.000e+0
Identity = 259/510 (50.78%), Postives = 351/510 (68.82%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVRMG+Y GA VYFV EG QG++DGG NI+EA W +VS M+ +GGTVIGSARC DF+T  GRLKAA NLI R I NL +IGGDGSLTGAN+F++EW+  + EL+++ +ID    +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL   +S   R+N++I++EGAID H + IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++   N   PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N K Y+ L      HRK   E             L +S  +  + ++  G  A GMNA +RS VR+ I+       +  GF+G  K  I  + W DV G    GGS++GT R LP++   + +A ++ ++ ++AL++IGGFE
Sbjct:   17 GKAIAVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFVHEGYQGMVDGGDNIKEATWENVSSMLQVGGTVIGSARCKDFRTHEGRLKAAHNLIQRGITNLCVIGGDGSLTGANLFREEWSGLLAELVQQGLIDEDAAQKYSALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSATRSRGSRLNIIIVAEGAIDRHGKAITSSIVKDLVVSRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLETTAN--TPACVVSLCGNQSVRLPLMECVQMTQEVQKAMDGKRFEEAVQLRGRSFENNLKTYKLL-----AHRKPESE-------------LPHS--NFNVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKGQIKEIKWADVGGWTGQGGSLLGTKRTLPAK-HIDKIAEQMRKYNINALLVIGGFE 503

HSP 2 Score: 196.823 bits (499), Expect = 0.000e+0
Identity = 103/257 (40.08%), Postives = 160/257 (62.26%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            +L + + KY+ F IP+ ++PATI+NN+PG   ++G+DT +N IV T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E+Y          DS  +LG+ +       P+    +  D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  510 QLNEARAKYEEFCIPLCMLPATISNNVPGTDLSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMV-FQPVVQLKDETDFVHRIPKEQWWLKLRPLMKILAKYKTSYDVS 765

HSP 3 Score: 121.324 bits (303), Expect = 8.357e-28
Identity = 105/383 (27.42%), Postives = 186/383 (48.56%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            + F       + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  ++ V +G +G   G   I+E  W+DV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +L              + L++     +  +++C  L +  L  +I N+  GTD++IG ++AL  I+E  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  +               N    AC++ + +  +V +P++    +T
Sbjct:  378 RSFENNLKTYKLLAHRKPESELPHSNF---NVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKG--QIKEIKWADVGGWTGQGGSLLGTKRTLPAKHID---KIAEQMRKYNINALLVIGGFEAL--------------ECLLQLNEARAKYEEFCIPLCM--LPATISNNVPGTDLSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIFEEPFDIRDLQANVEHLTEKMKTSIQR-GLVLRNENSNENYTTDFI--YQLYTEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWITKKLNESYRRGRVFANTEDSACLLGMRRRAMVFQPVVQLKDET 733

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Match: pfkpb (phosphofructokinase, platelet b [Source:ZFIN;Acc:ZDB-GENE-070912-634])

HSP 1 Score: 481.485 bits (1238), Expect = 0.000e+0
Identity = 259/519 (49.90%), Postives = 353/519 (68.02%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            K+I      G  I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QG++DGG NI+EA W  VS M+ +GGTVIGSARC DF+T  GRL AA NL+ R I NL +IGGDGSLTGAN+F++EW+  +DEL++   I     +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE+PP D WE+ +C+KL E ++  +R+N++I++EGAID +N+PIT  +IKD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A+  P  PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N   Y+ L+     HRK+  E             L +S  +  + ++  G  A GMNA +RS VR+ I        +  GF+G  K  I  + W DV G    GGS++GT R LP++   + +A ++    ++AL++IGGFE
Sbjct:    6 KYIENLSGAGKCIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGDNIKEASWESVSSMLQVGGTVIGSARCKDFRTHEGRLCAALNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELVQSGQISEDAAQTHSALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPERPPKDGWEEQMCQKLSENRADKKRLNIIIVAEGAIDQNNKPITTDHIKDLVVSRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEAS--PGTPACVVSLCGNQAVRVPLMECVQMTQEVQKAMDEKRFEEAVKLRGRSFENNLNTYKLLS-----HRKIDTE-------------LPHSSFN--VAVLNVGAPAAGMNAAVRSAVRVGITEGHTMFAVSDGFEGFYKGQIKEIKWGDVGGWTGQGGSLLGTKRTLPAK-HVDKIAEQMRIHNINALLVIGGFE 501

HSP 2 Score: 193.741 bits (491), Expect = 0.000e+0
Identity = 98/257 (38.13%), Postives = 157/257 (61.09%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY---------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   + K+    +P+V++PAT++NNIPG   ++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+   L+ +           DS  +LG+ +  +    P+    +  D+    P +QWW++LRPL++I+  +++ Y+ S
Sbjct:  508 ELQAARSKHAELCVPMVMVPATVSNNIPGSDLSIGADTAINAITDTCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQSNVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKIAAKAMQWISKKLKEFYRDGRVFANTEDSACLLGMRR-RALLFQPVVQLKDETDFVHRIPKEQWWLRLRPLMKILAKYKTSYDVS 763

HSP 3 Score: 120.168 bits (300), Expect = 2.031e-27
Identity = 102/383 (26.63%), Postives = 186/383 (48.56%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            + F    +  + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G T++ V +G +G   G   I+E  W DV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +  G              L+E +   S   + C  + +V +  ++ N+  G+D++IG ++A+  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  +    +          N    AC++ + +  ++ +P++    +T
Sbjct:  376 RSFENNLNTYKLLSHRKIDTELPHSSF---NVAVLNVGAPAAGMNAAVRSAVRVGITEGHTMFAVSDGFEGFYKG--QIKEIKWGDVGGWTGQGGSLLGTKRTLPAKHVD---KIAEQMRIHNINALLVIGGFEAYLG--------------LMELQAARSKHAELC--VPMVMVPATVSNNIPGSDLSIGADTAINAITDTCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQSNVEHLTEKMKTSIQR-GLVLRNENSNENYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKIAAKAMQWISKKLKEFYRDGRVFANTEDSACLLGMRRRALLFQPVVQLKDET 731

HSP 4 Score: 66.6254 bits (161), Expect = 8.235e-11
Identity = 53/198 (26.77%), Postives = 91/198 (45.96%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDE-------IKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD   I E         Q    L     D+       +  G + +NN    P  + +I  L     +  FD R   +GHV  GG  S+FDR L +R G+EA++    +LE        ++ L  + +  V
Sbjct:  158 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPERPPKDGWEEQMCQKLSENRADKKRLNIIIVAEGAIDQNN---KPITTDHIKDLVVS--RLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLEASPGTPACVVSLCGNQAVRV 346

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Match: pfkma (phosphofructokinase, muscle a [Source:ZFIN;Acc:ZDB-GENE-040912-135])

HSP 1 Score: 481.485 bits (1238), Expect = 0.000e+0
Identity = 253/510 (49.61%), Postives = 340/510 (66.67%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++ VR+GLY GA V+FV EG QGL+DGG +IR A W  VS M+ LGGTVIGSARC DF+TR GRLKAA NL+   I NL +IGGDGSLTGANIF+ EW+  + + + +  I   + K   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  D++ TTA SHQR F++EVMGRHCGYLAL+TA++  A +VFIPE PP+D WE+ LC++L E +++  R+N++I++EGAI+   QPIT   +KD++  R+  DTR T+LGHVQRGG P++FDR+L  R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+   M  G F D V +RG SF  N+  Y+ L  + P  +K                      ++  + I+  G    GMNA +R+ VR+ I      + +  GF+GL +  I P+ WN V      GGS +GT R+LP +   E ++  + ++ + AL+IIGGFE
Sbjct:   17 GRAIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVFFVHEGYQGLVDGGDHIRPATWESVSMMLQLGGTVIGSARCKDFQTREGRLKAAYNLVKLGITNLCVIGGDGSLTGANIFRTEWSDLLKDHVSKGKITKEEAKASSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDSITTTAQSHQRAFILEVMGRHCGYLALVTALACGADWVFIPEMPPDDGWEEHLCRRLTETRNAGSRLNVIIVAEGAINKEGQPITCDQLKDLVTKRLGFDTRATILGHVQRGGTPSAFDRVLGSRMGVEAVMALLEA--TPETPACVVSLTGNQAVRLPLMECVQVTKDVTIAMNEGRFDDAVKLRGRSFENNWNTYKLLAHIRPPDKK----------------------SNINVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQAKIEPITWNTVGDWTGKGGSDLGTKRLLPGKY-MEEISLNIAKYNIHALVIIGGFE 501

HSP 2 Score: 219.164 bits (557), Expect = 0.000e+0
Identity = 108/272 (39.71%), Postives = 169/272 (62.13%), Query Frame = 3

Query: 1611 EALSGQ-ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN-------YDN--DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS--FIMRHL 2390
            EA SG  E+ + ++KY+   IP+VIIPAT++NN+PG  F++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  L+ GAD +YI EE   +  L ++V  L  K+   +K GL+LRN  S+  Y + +IF L+ +EGKG+FDCR N +GH+  GG  + FDRN  T+ G +A++W+ + L++       + N  DS  +LG+ +     + P+    E  D+E   P  QWW+KLRP+++I+  ++   +TS   +M H+
Sbjct:  501 EAFSGGLEMVQGREKYEELCIPMVIIPATVSNNVPGSDFSIGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLSAGADAAYIFEEKFGIRDLERNVEHLVEKMKTTVKRGLILRNENSNSNYTTDFIFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKAVLWLTEKLKDCYRHGRIFANTPDSACVLGMRKRGLV-LQPLAELKEETDFEHRIPKSQWWLKLRPIMKILAKYKINLDTSEHALMEHV 771

HSP 3 Score: 114.005 bits (284), Expect = 1.635e-25
Identity = 100/353 (28.33%), Postives = 167/353 (47.31%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++A+L  G    GMNAAV++ VR+G+  G  +  V +G +GL      I    W+ V      GG+ +G+ R L  K      + + N+    I  LVIIGG  + +G     Q    Y +  I   +I +                ++ N+  G+D +IG ++AL  I    D +  + A + +R F+IE MG +CGYLA ++ +S+ A   +I  EK    + E ++   +E+ K++ +R  L++ +E +    N      +       +   D R  VLGH+Q+GG PT FDR  A + GA+A+  L +  +          N    ACV+ + K  +V +PL +   +T
Sbjct:  403 NVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQA--KIEPITWNTVGDWTGKGGSDLGTKRLLPGKYME---EISLNIAKYNIHALVIIGGFEAFSGGLEMVQGREKYEELCIPMVIIPA----------------TVSNNVPGSDFSIGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLSAGADAAYIFEEKFGIRDLERNVEHLVEKMKTTVKR-GLILRNENS--NSNYTTDFIFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKAVLWLTEKLKDCYRHGRIFANTPDSACVLGMRKRGLVLQPLAELKEET 731

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
Match: pfkp (phosphofructokinase, platelet [Source:NCBI gene;Acc:394825])

HSP 1 Score: 486.493 bits (1251), Expect = 0.000e+0
Identity = 261/516 (50.58%), Postives = 356/516 (68.99%), Query Frame = 2

Query:   89 QGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEAL----NVL-MPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +G +I VLTSGGD+QGMNAAV++VVRMG+Y  A VYF+ EG QG++DGG NI E  W  VS ++ +GGTVIGSARC  F+TR GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  +DEL++   I++   +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WED +C KL E ++  +R+N++I+SEGAID HN+ IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G + + AL++A   P  PACV+ +  N+ VR PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L    NV  +PK R  V  G  I K    K+      ++  + ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP++  F+ +A ++   K++AL++IGGFE
Sbjct:   15 KGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELMQNGKIEAEATREYAHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIIIVSEGAIDCHNKAITSDMVKDLVVKRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVDCVLALLEAT--PETPACVVSLCGNQAVRLPLMECVQMTQEVQKAMDERRFDDAVRLRGRSFENNLNTYKLLSHKKNVADLPKVRWAVALGLCIKK----KKLPFTLQSNFTVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKGQIKDIKWGDVGGWTGQGGSLLGTKRTLPAKY-FDKIAEQIRTHKINALLVIGGFE 523

HSP 2 Score: 204.527 bits (519), Expect = 0.000e+0
Identity = 103/259 (39.77%), Postives = 166/259 (64.09%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            E+ + + KYD F IP+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EG+G+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E Y         +DS+ +LG+ +    N++  P+    +  D++   P +QWW+K+RPL++I+  +++ Y+ S
Sbjct:  530 EMVEGRGKYDEFCIPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQWISKKLKETYRKGRVFANTDDSVCLLGMRRR---NLLFQPVTQLKDETDFKHRIPKEQWWLKMRPLMKILAKYKTSYDIS 785

HSP 3 Score: 108.612 bits (270), Expect = 1.291e-23
Identity = 94/325 (28.92%), Postives = 161/325 (49.54%), Query Frame = 2

Query:   47 LKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH 1015
            +K K+  F     F   ++AVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I++  W DV G    GG+++G+ R L  K      K A+ +   +I  L++IGG  +  G           + E++E R   D    ++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+  +        + L E   ++ +  LV+ +E + + +       Y       R   D R  VLGH+Q+GG P+ FDR    +  A+A+ 
Sbjct:  411 IKKKKLPFTLQSNF---TVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKG--QIKDIKWGDVGGWTGQGGSLLGTKRTLPAKYFD---KIAEQIRTHKINALLVIGGFEAYLG-----------LMEMVEGRGKYD----EFC--IPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFI--YQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQ 708

HSP 4 Score: 62.7734 bits (151), Expect = 1.616e-14
Identity = 47/172 (27.33%), Postives = 84/172 (48.84%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHY------IFK--LFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E        +   N L      + +  +++   VS    D H       + K  + K+ G   FD R   +GHV  GG  S+FDR L +R G++ ++
Sbjct:  159 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIII---VSEGAIDCHNKAITSDMVKDLVVKRLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVDCVL 323

HSP 5 Score: 37.7354 bits (86), Expect = 1.616e-14
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 2

Query: 1283 ERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            +RK F + S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I  + W  V  I+ +GG++IG+ R     ++ G    A  L++  ++ L +IGG
Sbjct:    5 DRKLFENLSGKGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGG 119

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
Match: pfkp (phosphofructokinase, platelet [Source:NCBI gene;Acc:394825])

HSP 1 Score: 483.411 bits (1243), Expect = 0.000e+0
Identity = 254/511 (49.71%), Postives = 348/511 (68.10%), Query Frame = 2

Query:   89 QGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +G +I VLTSGGD+QGMNAAV++VVRMG+Y  A VYF+ EG QG++DGG NI E  W  VS ++ +GGTVIGSARC  F+TR GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  +DEL++   I++   +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WED +C KL E ++  +R+N++I+SEGAID HN+ IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G + + AL++A   P  PACV+ +  N+ VR PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L                    S +K   D   ++  + ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP++  F+ +A ++   K++AL++IGGFE
Sbjct:   15 KGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELMQNGKIEAEATREYAHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIIIVSEGAIDCHNKAITSDMVKDLVVKRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVDCVLALLEAT--PETPACVVSLCGNQAVRLPLMECVQMTQEVQKAMDERRFDDAVRLRGRSFENNLNTYKLL--------------------SHKKNVADLPKSNFTVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKGQIKDIKWGDVGGWTGQGGSLLGTKRTLPAKY-FDKIAEQIRTHKINALLVIGGFE 502

HSP 2 Score: 204.142 bits (518), Expect = 0.000e+0
Identity = 104/265 (39.25%), Postives = 169/265 (63.77%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIMRHL 2390
            E+ + + KYD F IP+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EG+G+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E Y         +DS+ +LG+ +    N++  P+    +  D++   P +QWW+K+RPL++I+  +++ Y+ S   ++L
Sbjct:  509 EMVEGRGKYDEFCIPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQWISKKLKETYRKGRVFANTDDSVCLLGMRRR---NLLFQPVTQLKDETDFKHRIPKEQWWLKMRPLMKILAKYKTSYDISDAGKNL 770

HSP 3 Score: 108.997 bits (271), Expect = 8.250e-24
Identity = 95/338 (28.11%), Postives = 164/338 (48.52%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH 1015
            + F    +  + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I++  W DV G    GG+++G+ R L  K      K A+ +   +I  L++IGG  +  G           + E++E R   D    ++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+  +        + L E   ++ +  LV+ +E + + +       Y       R   D R  VLGH+Q+GG P+ FDR    +  A+A+ 
Sbjct:  377 RSFENNLNTYKLLSHKKNVADLPKSNF---TVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKG--QIKDIKWGDVGGWTGQGGSLLGTKRTLPAKYFD---KIAEQIRTHKINALLVIGGFEAYLG-----------LMEMVEGRGKYD----EFC--IPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFI--YQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQ 687

HSP 4 Score: 62.3882 bits (150), Expect = 2.118e-14
Identity = 47/172 (27.33%), Postives = 84/172 (48.84%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHY------IFK--LFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E        +   N L      + +  +++   VS    D H       + K  + K+ G   FD R   +GHV  GG  S+FDR L +R G++ ++
Sbjct:  159 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIII---VSEGAIDCHNKAITSDMVKDLVVKRLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVDCVL 323

HSP 5 Score: 37.7354 bits (86), Expect = 2.118e-14
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 2

Query: 1283 ERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            +RK F + S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I  + W  V  I+ +GG++IG+ R     ++ G    A  L++  ++ L +IGG
Sbjct:    5 DRKLFENLSGKGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGG 119

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
Match: ENSXETT00000054028.1 (phosphofructokinase, muscle [Source:NCBI gene;Acc:100216266])

HSP 1 Score: 482.641 bits (1241), Expect = 0.000e+0
Identity = 266/533 (49.91%), Postives = 352/533 (66.04%), Query Frame = 2

Query:   23 QTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            QTD      +SK       G   G +IAVLTSGGD+QGMNAAV++VVR+G+Y GA VYFV EG QGL+DGG NI+EA W  VS M+ LGGTVIGSARC DF+ R GRLKAA NL+ + I NL +IGGDGSLTGA+ F+ EW++ + +L++   I + + K   +LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLALITA++  A +VFIPE PP +NWE+ LC++L E +    R+N++I++EGAID++ +PIT+  +KD+++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ +R PLM+CV  T D+   M    F + + +RG SF  N+K+Y  L  + P   K        S Y+              + I+  G  A GMNA +RS VR+ I        +  GF+GL K  +  + W+ V G    GGS +GT R LP +  FE +++ +  + +  LIIIGGFE
Sbjct:   23 QTDKTMLASRSKHHDAQTMG--TGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGAKVYFVHEGYQGLVDGGDNIKEATWESVSMMLQLGGTVIGSARCQDFRGREGRLKAACNLVKKGITNLCVIGGDGSLTGADTFRTEWSNLLADLVKAGRITADESKKSSHLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALITALACGADWVFIPESPPEENWEEHLCRRLSETRGRGSRLNIIIVAEGAIDLNGKPITSDLVKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAIRLPLMECVQVTKDVTQAMTEKRFDEALKLRGRSFENNWKVYRTLAHVRPTATK--------SGYN--------------LAILNVGAPAAGMNAAVRSTVRIGIIQGHTMFAVHDGFEGLAKGQLEEIGWSGVGGWTGQGGSRLGTKRTLPKKY-FEDISNTISNYNIHGLIIIGGFE 528

HSP 2 Score: 202.986 bits (515), Expect = 0.000e+0
Identity = 105/264 (39.77%), Postives = 159/264 (60.23%), Query Frame = 3

Query: 1611 EALSGQ-ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDN---------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EA +G  EL + + KY+   IP+V+IPAT++NN+PG  F++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM   A GAD +YI EE  ++  L  +V  L  K+   +K GL+LRN   +  Y + +IF L+ +EGKG+FDCR N +GH+  GG  + FDRN GT+ G +A++W+   ++             DS  +LG+ +  S    P+    E  D+E   P +QWW+KLRP+L+I+  +    +TS
Sbjct:  528 EAFTGSLELMEGRCKYEELCIPIVVIPATVSNNVPGSDFSIGADTALNTITVTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGFAAGADAAYIYEEQFSIHDLQINVEHLTEKMKTTVKRGLILRNERCNDNYSTDFIFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWMTGKVKECSRHGRIFANTPDSACLLGM-RKRSLVFQPLVELKEQTDFEHRLPKEQWWLKLRPILKILAKYNISLDTS 790

HSP 3 Score: 110.538 bits (275), Expect = 2.770e-24
Identity = 105/362 (29.01%), Postives = 175/362 (48.34%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAI------------HALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            P     G+++A+L  G  + GMNAAV+S VR+G+  G T++ V +G +GL  G L   E  WS V G    GG+ +G+ R L  K        +  + +  I  L+IIGG  + TG+            EL+E +      ++ C  + IV +  ++ N+  G+D +IG ++AL  I    D +  +A  + +R F+IE MG +CGYLA +   ++ A   +I E+  + +      + L E   +  +  L++ +E   D ++      +       +   D R  VLGH+Q+GG PT FDR    + GA+A+            H  I A  N    AC++ + K  +V +PL++   +T
Sbjct:  422 PTATKSGYNLAILNVGAPAAGMNAAVRSTVRIGIIQGHTMFAVHDGFEGLAKGQLE--EIGWSGVGGWTGQGGSRLGTKRTLPKKYFE---DISNTISNYNIHGLIIIGGFEAFTGSL-----------ELMEGRCK---YEELC--IPIVVIPATVSNNVPGSDFSIGADTALNTITVTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGFAAGADAAYIYEEQFSIHDLQINVEHLTEKMKTTVKRGLILRNERCNDNYSTDFI--FNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWMTGKVKECSRHGRIFA--NTPDSACLLGMRKRSLVFQPLVELKEQT 758

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
Match: pfkp (phosphofructokinase, platelet [Source:NCBI gene;Acc:394825])

HSP 1 Score: 482.641 bits (1241), Expect = 0.000e+0
Identity = 254/511 (49.71%), Postives = 348/511 (68.10%), Query Frame = 2

Query:   89 QGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +G +I VLTSGGD+QGMNAAV++VVRMG+Y  A VYF+ EG QG++DGG NI E  W  VS ++ +GGTVIGSARC  F+TR GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  +DEL++   I++   +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WED +C KL E ++  +R+N++I+SEGAID HN+ IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G + + AL++A   P  PACV+ +  N+ VR PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L                    S +K   D   ++  + ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP++  F+ +A ++   K++AL++IGGFE
Sbjct:   15 KGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELMQNGKIEAEATREYAHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIIIVSEGAIDCHNKAITSDMVKDLVVKRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVDCVLALLEAT--PETPACVVSLCGNQAVRLPLMECVQMTQEVQKAMDERRFDDAVRLRGRSFENNLNTYKLL--------------------SHKKNVADLPKSNFTVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKGQIKDIKWGDVGGWTGQGGSLLGTKRTLPAKY-FDKIAEQIRTHKINALLVIGGFE 502

HSP 2 Score: 193.356 bits (490), Expect = 0.000e+0
Identity = 103/277 (37.18%), Postives = 166/277 (59.93%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLV------------------LRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            E+ + + KYD F IP+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLV                  LRN  S+  Y + +I++L+ +EG+G+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E Y         +DS+ +LG+ +    N++  P+    +  D++   P +QWW+K+RPL++I+  +++ Y+ S
Sbjct:  509 EMVEGRGKYDEFCIPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRPRLFIDQPPIQTTPRLLRNENSNENYTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQWISKKLKETYRKGRVFANTDDSVCLLGMRR---RNLLFQPVTQLKDETDFKHRIPKEQWWLKMRPLMKILAKYKTSYDIS 782

HSP 3 Score: 103.605 bits (257), Expect = 4.723e-22
Identity = 98/354 (27.68%), Postives = 164/354 (46.33%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDM--------------HNQPITAQ--YXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH 1015
            + F    +  + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I++  W DV G    GG+++G+ R L  K      K A+ +   +I  L++IGG  +  G           + E++E R   D    ++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+  +        + L E   ++ +  LV+     ID                N+  T    Y       R   D R  VLGH+Q+GG P+ FDR    +  A+A+ 
Sbjct:  377 RSFENNLNTYKLLSHKKNVADLPKSNF---TVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKG--QIKDIKWGDVGGWTGQGGSLLGTKRTLPAKYFD---KIAEQIRTHKINALLVIGGFEAYLG-----------LMEMVEGRGKYD----EFC--IPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRPRLFIDQPPIQTTPRLLRNENSNENYTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQ 705

HSP 4 Score: 62.7734 bits (151), Expect = 1.812e-14
Identity = 47/172 (27.33%), Postives = 84/172 (48.84%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHY------IFK--LFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E        +   N L      + +  +++   VS    D H       + K  + K+ G   FD R   +GHV  GG  S+FDR L +R G++ ++
Sbjct:  159 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIII---VSEGAIDCHNKAITSDMVKDLVVKRLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVDCVL 323

HSP 5 Score: 37.7354 bits (86), Expect = 1.812e-14
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 2

Query: 1283 ERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            +RK F + S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I  + W  V  I+ +GG++IG+ R     ++ G    A  L++  ++ L +IGG
Sbjct:    5 DRKLFENLSGKGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGG 119

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Query:   89 QGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +G +I VLTSGGD+QGMNAAV++VVRMG+Y  A VYF+ EG QG++DGG NI E  W  VS ++ +GGTVIGSARC  F+TR GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  +DEL++   I++   +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WED +C KL E ++  +R+N++I+SEGAID HN+ IT+  +KD+++ R+  DTR+T+LGHVQRGG P++FDRILA R G + + AL++A   P  PACV+ +  N+ VR PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L                    S +K   D   ++  + ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP++  F+ +A ++   K++AL++IGGFE
Sbjct:   15 KGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELMQNGKIEAEATREYAHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIIIVSEGAIDCHNKAITSDMVKDLVVKRLGFDTRVTILGHVQRGGTPSAFDRILASRMGVDCVLALLEA--TPETPACVVSLCGNQAVRLPLMECVQMTQEVQKAMDERRFDDAVRLRGRSFENNLNTYKLL--------------------SHKKNVADLPKSNFTVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKGQIKDIKWGDVGGWTGQGGSLLGTKRTLPAKY-FDKIAEQIRTHKINALLVIGGFE 502

HSP 2 Score: 193.741 bits (491), Expect = 0.000e+0
Identity = 99/261 (37.93%), Postives = 163/261 (62.45%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY----------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            +L + +  Y+ + IP+ ++PATI+NN+PG   ++G+DT +N I+ T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EG+G+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K + E Y           +DS+ +LG+ +    N++  P+    +  D++   P +QWW+K+RPL++I+  +++ Y+ S
Sbjct:  509 QLAEARGIYEEYCIPMCVLPATISNNVPGTDLSLGADTALNAIMETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQWISKKLKETYRKVPGRVFANTDDSVCLLGMRRR---NLLFQPVTQLKDETDFKHRIPKEQWWLKMRPLMKILAKYKTSYDIS 766

HSP 3 Score: 116.701 bits (291), Expect = 3.725e-26
Identity = 95/337 (28.19%), Postives = 162/337 (48.07%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH 1015
            + F    +  + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I++  W DV G    GG+++G+ R L  K      K A+ +   +I  L++IGG                  + +++      I ++YC  + +  L  +I N+  GTD+++G ++AL  IME  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+  +        + L E   ++ +  LV+ +E + + +       Y       R   D R  VLGH+Q+GG P+ FDR    +  A+A+ 
Sbjct:  377 RSFENNLNTYKLLSHKKNVADLPKSNF---TVAVLNVGAPAAGMNAAVRSAVRVGITEGHKMLAVNDGFEGFAKG--QIKDIKWGDVGGWTGQGGSLLGTKRTLPAKYFD---KIAEQIRTHKINALLVIGG--------------FEAFESMVQLAEARGIYEEYCIPMCV--LPATISNNVPGTDLSLGADTALNAIMETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEESFDIRELQANVEHLTEKMKTSIQRGLVLRNENSNENYTTDFI--YQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAVQ 687

HSP 4 Score: 62.3882 bits (150), Expect = 2.243e-14
Identity = 47/172 (27.33%), Postives = 84/172 (48.84%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHY------IFK--LFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E        +   N L      + +  +++   VS    D H       + K  + K+ G   FD R   +GHV  GG  S+FDR L +R G++ ++
Sbjct:  159 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEYPPEEGWEDMMCNKLSENRARKKRLNIII---VSEGAIDCHNKAITSDMVKDLVVKRLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVDCVL 323

HSP 5 Score: 37.7354 bits (86), Expect = 2.243e-14
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 2

Query: 1283 ERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            +RK F + S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I  + W  V  I+ +GG++IG+ R     ++ G    A  L++  ++ L +IGG
Sbjct:    5 DRKLFENLSGKGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVQAKVYFIYEGYQGMVDGGDNIEEVSWESVSSILQVGGTVIGSARCKAFRTREGRLQAAYNLVQRGITNLCVIGG 119

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
Match: Pfkp (phosphofructokinase, platelet [Source:MGI Symbol;Acc:MGI:1891833])

HSP 1 Score: 474.167 bits (1219), Expect = 0.000e+0
Identity = 255/510 (50.00%), Postives = 346/510 (67.84%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QGL+DGG NI EA W  VS ++ +GGT+IGSARC  F++R GRLKAA NL    I NL +IGGDGSLTGAN+F++EW+  ++EL     ID+   +    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP ++WE+++C KL E ++  +R+N++I+SEGAIDM N+PIT++ IK++++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+Q  M    F + V +RG  F  N   Y+ L + +P          +I K            ++  + ++  G  A GMNA +RS VR+ IA+      I  GF+G     I  + W DV G    GGSI+GT R LP +   E +A ++    ++AL+IIGGFE
Sbjct:   23 GKAIGVLTSGGDAQGMNAAVRAVVRMGIYTGAKVYFIYEGYQGLVDGGSNIVEAKWDCVSSILQVGGTIIGSARCKAFRSREGRLKAACNLARLGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGDIDNDTVQKYSYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAIDMQNKPITSEKIKELVVKNLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEA--TPETPACVVSLRGNQAVRLPLMECVQMTQDVQKAMDERRFKEAVKLRGRRFEGNLNTYKRLAIKLPDE--------KIVK------------SNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANGQIKEIGWADVGGWTGQGGSILGTKRTLPGKY-LEKIAEQMHSHSINALLIIGGFE 509

HSP 2 Score: 199.134 bits (505), Expect = 0.000e+0
Identity = 102/256 (39.84%), Postives = 160/256 (62.50%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN--------YDNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   ++K++ F +P+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MGALA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+   L+           +DSI +LG+ +       P+    +  D+E   P +QWW+KLRP+++I+  +E+ Y+ S
Sbjct:  516 ELAAAREKHEAFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQRGLVLRNESCSVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSQGTGKKFVSDDSICVLGICK-RDLLFQPVAELKKVTDFEHRIPKEQWWLKLRPIMKILAKYEASYDMS 770

HSP 3 Score: 119.013 bits (297), Expect = 4.127e-27
Identity = 104/359 (28.97%), Postives = 182/359 (50.70%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA---IHALIDAAQ--------NPSIPACVICIVKNEIVRKPLMDCVSKTLDIQN 1135
            ++AV+  G  + GMNAAV+S VR+G+  G  ++ + +G +G  +G   I+E  W+DV G    GG+++G+ R L  K      K A+ +    I  L+IIGG  +  G              L+E        + +C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA + A+++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E      N      Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A   I A +  +Q        + SI  CV+ I K +++ +P+ + + K  D ++
Sbjct:  411 NVAVINVGAPAAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANG--QIKEIGWADVGGWTGQGGSILGTKRTLPGKYLE---KIAEQMHSHSINALLIIGGFEAYLG--------------LLELAAAREKHEAFC--VPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQR-GLVLRNESC--SVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSQGTGKKFVSDDSI--CVLGICKRDLLFQPVAE-LKKVTDFEH 742

HSP 4 Score: 65.855 bits (159), Expect = 1.781e-10
Identity = 57/195 (29.23%), Postives = 93/195 (47.69%), Query Frame = 3

Query: 1626 QELWKNKD-------KYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLE-----SLNQDVNILKAKLFDEI--KYGLVLRNNVSHHPYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +EL +N D       KY      VV +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + AL  GAD  ++ E     +      L    N  + K  + I    G +   ++ + P  S  I +L  K  G   FD R   +GHV  GG  S+FDR L +R G+EA+I
Sbjct:  145 EELARNGDIDNDTVQKYSYLN--VVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAI---DMQNKPITSEKIKELVVKNLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVEAVI 330

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
Match: Pfkl (phosphofructokinase, liver, B-type [Source:MGI Symbol;Acc:MGI:97547])

HSP 1 Score: 473.781 bits (1218), Expect = 0.000e+0
Identity = 257/511 (50.29%), Postives = 346/511 (67.71%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELI-ERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +I VLTSGGD+QGMNAAV++V RMG+Y GA V+ + EG +GL++GG NI+ A W  VS ++ LGGT+IGSARC  F TR GRL AA NL+   I NL +IGGDGSLTGANIF+ EW S ++EL+ E K+ +S  + Y  +L I GLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL++A++S A ++FIPE PP D WE+ +C++L E +S   R+N++II+EGAID H +PI++ Y+KD+++ R+  DTR+TVLGHVQRGG P++FDRIL+ + G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+Q  M    F + + +RG SF  N+KIY+ L      H+K+  E +  S                 + I+  G  A GMNA +RS VR  I+       +  GF+GL K  +  + W+DV G +  GGS++GT R LP +   E +   L  + + AL++IGGFE
Sbjct:   15 GKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANWLSVSNIIQLGGTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELVKEGKISESTAQNYA-HLTIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEA--TPDTPACVVSLSGNQSVRLPLMECVQVTKDVQKAMDEERFDEAIQLRGRSFENNWKIYKLL-----AHQKVSKEKSNFS-----------------LAILNVGAPAAGMNAAVRSAVRTGISEGHTVYIVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLP-KPHLEAIVENLRTYNIHALLVIGGFE 499

HSP 2 Score: 196.823 bits (499), Expect = 0.000e+0
Identity = 92/247 (37.25%), Postives = 152/247 (61.54%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN-------YDN--DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRII 2342
            +L + + +Y+   I + +IPATI+NN+PG  F++GSDT VN  + + D +K  + GT+ R++I ET+G  CGYL T+  +A GAD +Y+ E+  N+  L  +V  +  K+  +I+ GLVLRN   H  Y + +++ L+  EG+G+FDCR N +GH+  GG  + FDRN GT+ G++A++WV + L +       + N  DS  ++GL +   A   P+    +  D+E   P +QWW+ LR +L+++
Sbjct:  506 QLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLRDVYRKGRVFANAPDSACVIGLRKKVVA-FSPVTELKKETDFEHRMPREQWWLNLRLMLKML 751

HSP 3 Score: 132.109 bits (331), Expect = 3.514e-31
Identity = 116/382 (30.37%), Postives = 191/382 (50.00%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKA-AKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLV--GSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAI----HALIDAAQ------NPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLM-RNGIFVDL-VMMRGPSFYR 1195
            S+A+L  G  + GMNAAV+S VR G+  G TVY V +G +GL  G   ++E  W DV+G +  GG+++G+ R L        L+A  +NL    I  L++IGG  +  G           + +L+E       + +Y + L IV  V   +I N+  GTD ++G ++A+   ME+ D +  +A  + +R F++E MG +CGYLA +T I+  A   ++ E P N +      + + E   ++ +  LV+ +E   + +       Y       R   D R  VLGH+Q+GG PT FDR    + G +A+      L D  +      N    ACVI + K  +   P+ + + K  D ++ M R   +++L +M++  + YR
Sbjct:  401 SLAILNVGAPAAGMNAAVRSAVRTGISEGHTVYIVHDGFEGLAKG--QVQEVGWHDVAGWLGRGGSMLGTKRTLP----KPHLEAIVENLRTYNIHALLVIGGFEAYEG-----------VLQLVE------ARGRY-EELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFL--YNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLRDVYRKGRVFANAPDSACVIGLRKKVVAFSPVTE-LKKETDFEHRMPREQWWLNLRLMLKMLAHYR 755

HSP 4 Score: 69.707 bits (169), Expect = 1.175e-11
Identity = 55/193 (28.50%), Postives = 91/193 (47.15%), Query Frame = 3

Query: 1689 ATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
             +I N+  G    +G+D+ ++ I+  +D +   +   Q R ++ E +G +CGYL  + ALA GAD  +I E       E    E L +  +         I  G + R+     P  S Y+  L  Q  +  FD R   +GHV  GG  S+FDR L ++ G+EA++    +LE   +    ++ LS + S  +
Sbjct:  163 GSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQ-RTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHG---KPISSSYVKDLVVQ--RLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVM---ALLEATPDTPACVVSLSGNQSVRL 346

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
Match: Pfkp (phosphofructokinase, platelet [Source:MGI Symbol;Acc:MGI:1891833])

HSP 1 Score: 472.626 bits (1215), Expect = 0.000e+0
Identity = 255/510 (50.00%), Postives = 346/510 (67.84%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QGL+DGG NI EA W  VS ++ +GGT+IGSARC  F++R GRLKAA NL    I NL +IGGDGSLTGAN+F++EW+  ++EL     ID+   +    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP ++WE+++C KL E ++  +R+N++I+SEGAIDM N+PIT++ IK++++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+Q  M    F + V +RG  F  N   Y+ L + +P          +I K            ++  + ++  G  A GMNA +RS VR+ IA+      I  GF+G     I  + W DV G    GGSI+GT R LP +   E +A ++    ++AL+IIGGFE
Sbjct:   23 GKAIGVLTSGGDAQGMNAAVRAVVRMGIYTGAKVYFIYEGYQGLVDGGSNIVEAKWDCVSSILQVGGTIIGSARCKAFRSREGRLKAACNLARLGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGDIDNDTVQKYSYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAIDMQNKPITSEKIKELVVKNLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEA--TPETPACVVSLRGNQAVRLPLMECVQMTQDVQKAMDERRFKEAVKLRGRRFEGNLNTYKRLAIKLPDE--------KIVK------------SNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANGQIKEIGWADVGGWTGQGGSILGTKRTLPGKY-LEKIAEQMHSHSINALLIIGGFE 509

HSP 2 Score: 192.2 bits (487), Expect = 0.000e+0
Identity = 102/255 (40.00%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1632 LWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN--------YDNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            L + ++ ++ F IP+ ++PATI+N+IPG   ++G DT +NVIV T D +K  + GT+ R++I ET+G  CGYL  MGALA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+   L+           +DSI +LG+ +       P+    +  D+E   P +QWW+KLRP+++I+  +E+ Y+ S
Sbjct:  517 LSEARNNHEEFCIPICVLPATISNDIPGSDISIGCDTALNVIVETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQRGLVLRNESCSVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSQGTGKKFVSDDSICVLGICK-RDLLFQPVAELKKVTDFEHRIPKEQWWLKLRPIMKILAKYEASYDMS 770

HSP 3 Score: 124.02 bits (310), Expect = 1.392e-28
Identity = 107/359 (29.81%), Postives = 185/359 (51.53%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA---IHALIDAAQ--------NPSIPACVICIVKNEIVRKPLMDCVSKTLDIQN 1135
            ++AV+  G  + GMNAAV+S VR+G+  G  ++ + +G +G  +G   I+E  W+DV G    GG+++G+ R L  K      K A+ +    I  L+IIGG     GA +  +   ++              +++C  + I  L  +I ND  G+D++IG ++AL  I+E  D +  +A  + +R F+IE MG +CGYLA + A+++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E      N      Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A   I A +  +Q        + SI  CV+ I K +++ +P+ + + K  D ++
Sbjct:  411 NVAVINVGAPAAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANG--QIKEIGWADVGGWTGQGGSILGTKRTLPGKYLE---KIAEQMHSHSINALLIIGGFEGYHGAIMLSEARNNH--------------EEFC--IPICVLPATISNDIPGSDISIGCDTALNVIVETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQR-GLVLRNESC--SVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSQGTGKKFVSDDSI--CVLGICKRDLLFQPVAE-LKKVTDFEH 742

HSP 4 Score: 65.4698 bits (158), Expect = 2.132e-10
Identity = 57/195 (29.23%), Postives = 93/195 (47.69%), Query Frame = 3

Query: 1626 QELWKNKD-------KYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLE-----SLNQDVNILKAKLFDEI--KYGLVLRNNVSHHPYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            +EL +N D       KY      VV +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + AL  GAD  ++ E     +      L    N  + K  + I    G +   ++ + P  S  I +L  K  G   FD R   +GHV  GG  S+FDR L +R G+EA+I
Sbjct:  145 EELARNGDIDNDTVQKYSYLN--VVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAI---DMQNKPITSEKIKELVVKNLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVEAVI 330

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
Match: Pfkm (phosphofructokinase, muscle [Source:MGI Symbol;Acc:MGI:97548])

HSP 1 Score: 468.774 bits (1205), Expect = 0.000e+0
Identity = 253/510 (49.61%), Postives = 345/510 (67.65%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +IREA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  +++L +   I + +      LNIVGLVGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P     V +G   +                 + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +   E +++ + +F +  L+IIGGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPP----VSKGGLHT-----------------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-NLEQISANITKFNIQGLVIIGGFE 500

HSP 2 Score: 196.052 bits (497), Expect = 0.000e+0
Identity = 105/259 (40.54%), Postives = 155/259 (59.85%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +       P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  505 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALV-FQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 762

HSP 3 Score: 125.946 bits (315), Expect = 2.779e-29
Identity = 112/363 (30.85%), Postives = 186/363 (51.24%), Query Frame = 2

Query:   71 IPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            +  GG   H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K      + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D +IG ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  395 VSKGGL--HTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQT 730

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Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVR+G++ GA V+FV EG QGL+DGG +IREA W  VS M+ LGGTVIGSARC DF+ R GRL+AA NL+ R I NL +IGGDGSLTGA+ F+ EW+  +++L +   I + +      LNIVGLVGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR FV+EVMGRHCGYLAL+T++S  A +VFIPE PP+D+WE+ LC++L E ++   R+N++I++EGAID + +PIT++ IK++++ R+  DTR+TVLGHVQRGG P++FDRIL  R G EA+ AL++    P  PACV+ +  N+ VR PLM+CV  T D+   M    F + + +RG SF  N+++Y+ L  + P     V +G   +                 + +M  G  A GMNA +RS VR+ +      + +  GF+GL K  I    W+ V G    GGS +GT R LP +   E +++ + +F +  L+IIGGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEG--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPP----VSKGGLHT-----------------VAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKK-NLEQISANITKFNIQGLVIIGGFE 500

HSP 2 Score: 196.052 bits (497), Expect = 0.000e+0
Identity = 105/259 (40.54%), Postives = 155/259 (59.85%), Query Frame = 3

Query: 1623 GQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            G EL + + ++D   IP V+IPAT++NN+PG  F++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GLVLRN   +  Y + +IF L+ +EGKGIFD R N +GH+  GG  + FDRN  T+ G +A+ W+   + E+Y N        DS  +LG+ +       P+    +  D+E   P +QWW+KLRP+L+I+  +E   +TS
Sbjct:  505 GLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALV-FQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTS 762

HSP 3 Score: 125.946 bits (315), Expect = 2.779e-29
Identity = 112/363 (30.85%), Postives = 186/363 (51.24%), Query Frame = 2

Query:   71 IPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH----ALIDAAQNPSIPA------CVICIVKNEIVRKPLMDCVSKT 1120
            +  GG   H++AV+  G  + GMNAAV+S VR+GL  G  V  V +G +GL  G   I EA WS V G    GG+ +G+ R L  K      + + N+    I  LVIIGG  + TG             EL+E RK  D +    C  +  V +  ++ N+  G+D +IG ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + + ++   +++ K++ +R  LV+ +E   + +       +       +   D+R  VLGH+Q+GG+PT FDR  A + GA+A++     + ++ +N  I A      CV+ + K  +V +P+ +   +T
Sbjct:  395 VSKGGL--HTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKG--QIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLE---QISANITKFNIQGLVIIGGFEAYTGGL-----------ELMEGRKQFDEL----C--IPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKR-GLVLRNEKCNENYTTDFI--FNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQT 730

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BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Match: sp|Q27778|PFKA_SCHMA (ATP-dependent 6-phosphofructokinase OS=Schistosoma mansoni OX=6183 GN=PFK PE=1 SV=1)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 279/521 (53.55%), Postives = 372/521 (71.40%), Query Frame = 2

Query:   59 EGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            EGK++  G F G  IAVLTSGGD+QGMNAAV++VVRMG+YCG  V+F+ EG QGL+DGG NI+EA W+DVSG++ LGGT IGSARC+DF+ R GRLKAA+NL+  QI NLV+IGGDGSLTGAN+F+ EW+S ++EL+    I +   K    LNIVGLVGSIDNDFCGTDMTIG +SAL RI+EATDA+ TTAHSHQRCF++EVMGRHCGYLAL+ +++ EA +VFIPE PP D+W + LC KL   +   +RVN+++++EGAID   +PIT + +K++I+  +++DTRITVLGHVQRGG+P++FDRIL  R GAEA+ AL+DA ++P++P+CVI +  N+ VR PL+ CV +T  +   M+   F   V +RG SF  N   Y                  ++SK  + ++ + +S  + RIGI+  G  ACG+NA+IR   R+ I      IGI  GF GL K   + + W DVRG + MGGS++GT R  P+ +G + VA++  E KLS L+IIGGFE
Sbjct:    6 EGKYVARGQFTGECIAVLTSGGDAQGMNAAVRAVVRMGIYCGCRVFFIREGYQGLVDGGQNIQEASWADVSGILQLGGTKIGSARCMDFRERYGRLKAAENLVKNQITNLVVIGGDGSLTGANLFRAEWSSLLEELVTSNKISAESAKQFHRLNIVGLVGSIDNDFCGTDMTIGADSALHRIIEATDAISTTAHSHQRCFILEVMGRHCGYLALVASMACEADWVFIPEMPPTDDWREKLCHKLRMNREHGQRVNIIMVAEGAIDRACKPITCEIVKNLIVSELQLDTRITVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDADRDPNLPSCVISLDGNQAVRVPLVKCVDRTRQVAEAMKACDFDHAVELRGTSFMNNLATY-----------------IKLSKIEQPRQSVMSSENNLRIGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKGDASEIQWADVRGWVGMGGSMLGTRRDTPNGLGIDKVAAKFKELKLSGLLIIGGFE 509

HSP 2 Score: 211.846 bits (538), Expect = 0.000e+0
Identity = 105/260 (40.38%), Postives = 164/260 (63.08%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKD--------MLENYDNDSIVILGLSQDTSANVIPIHTQF-ENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            EL + ++KY    IP+ ++PATI+NN+PG  F++G DT +N I + LD +K  ++GT+ R+++ ET+G  CGYL TM ALAGGAD +YI EE   ++ L +DV  L+AK+ D +K GLVLR ++  +   S +I +L+ QEG+GIFDCR N +GH+  G   S FDR+LGT+   +AI W+ +        +L++     ++ +     +S N+     +  E+ D+    P ++WW+ LRPL+RI+  H+S YE+  IM
Sbjct:  516 ELVEGREKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVVHLRAKIDDNVKRGLVLRADMLINTITSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWLDEQINANIVQILQSIHQTFMLYINWYCTSSDNLFKYSLELREHTDFVHRLPKEEWWLSLRPLMRIMAKHDSLYESESIM 775

HSP 3 Score: 108.227 bits (269), Expect = 8.382e-23
Identity = 93/312 (29.81%), Postives = 156/312 (50.00%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLV-GSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXX--XXXVLGHVQRGGNPTSFDRILALREGAEAIHAL 1021
            I ++  G  + G+NA ++   R+G+  G  V  + EG  GL+ G  +  E  W+DV G V +GG+++G+ R  D     G  K A    + ++  L+IIGG            E    + EL+E +      +KY +    + +V  +I N+  GTD ++G ++AL  I    D +  +A  + +R FV+E MG +CGYLA ++A++  A   +I E+P   D+  +D+   L      N +  LV+ +    DM    IT+++I  +     +     R  VLGH+Q+G  P+ FDR L  +  ++AI  L
Sbjct:  411 IGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKG--DASEIQWADVRGWVGMGGSMLGTRR--DTPNGLGIDKVAAKFKELKLSGLLIIGG-----------FEAYECMIELVEGR------EKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVV-HLRAKIDDNVKRGLVLRA----DMLINTITSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWL 696

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BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Match: sp|P52034|PFKA_DROME (ATP-dependent 6-phosphofructokinase OS=Drosophila melanogaster OX=7227 GN=Pfk PE=2 SV=2)

HSP 1 Score: 494.967 bits (1273), Expect = 0.000e+0
Identity = 268/519 (51.64%), Postives = 357/519 (68.79%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +F+  G  +   +AV TSGGDSQGMNAAV++ VRM +Y G  VYF+ EG QG++DGG  I+EA W+ VS +++ GGT+IGSARC DF+ R GRLKAA NLI R I NLV+IGGDGSLTGAN+F+QEW+S +DEL++ K I + +++    L+IVGLVGSIDNDFCGTDMTIG ++AL RI+EA DA+ +TA+SHQR F++EVMGRHCGYLAL+  ++ EA F+FIPE PP  +W D LC +L + +S+ +R+N+VI++EGA+D    PITA+ +K +I +R+K D RITVLGHVQRGGNP++FDRILA R GAEA  AL++A ++ S+P  VI +  N+ VR PLM+CV +T  +   M    + D V +RG SF RN + Y+ L  L P       +G  I  Y              R+ +M  G  ACGMNA +RS VR AI       GI  G +GL    +  L W+DV G +  GG+ +GT R LP +  F+ +A+RL EFK+  L+IIGGFE
Sbjct:    8 RFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKD-SVPV-VISLDGNQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGIEGY--------------RLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFE 509

HSP 2 Score: 169.088 bits (427), Expect = 0.000e+0
Identity = 94/255 (36.86%), Postives = 157/255 (61.57%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE---------NADYEKYRPLDQWWMKLRPLLRIIQTHESQYE 2366
            ++   +D Y  F IP+V+IP+TI+NN+PG  F++G DT +N I    D ++  + GT+ R+++ ET+G  CGYL T+  LAGGAD +YI EE  +++ L QDV  + +K+ + +  GL+LRN  +   Y + +I++L+ +EGKG+F CR N +GH+  GG  + FDRN+GT+   + + W+   ++   + + V+   S DT+  +  +  Q+            ++++  P  QWW++LRPLLRI+  H+S YE
Sbjct:  516 QIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYE 770

HSP 3 Score: 134.42 bits (337), Expect = 4.525e-31
Identity = 123/391 (31.46%), Postives = 192/391 (49.10%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAG-GFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA-------IHALIDA-----AQNPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            E +L+T      LK  +  F   G G +G+ +AV+  G  + GMNAAV+S VR  +Y G  VY + +G++GLI G  N+RE  WSDVSG V  GG  +G+ R L    FK  + RLK  K      I  L+IIGG  S   A     +  +Y               ++C  + IV +  +I N+  GT+ ++G ++ L  I E  D +  +A  + +R FVIE MG +CGYLA +  ++  A   +I  EK    + + D   +  K+ E  S      L++ +E A + ++      Y       +     R+ +LGH+Q+GG+PT FDR +  +  A+        I A IDA      ++P   A ++ IV  +    PL+D +++T
Sbjct:  381 ERNLETYKMLTRLKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAG--NVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFK------IQGLLIIGGFESYHAAGQIADQRDNY--------------PQFC--IPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFSIKDLQQDVYHMASKMAEGVSR----GLILRNEKASENYSTDFI--YRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDT-ATLLGIVSRQYRFSPLVDLIAET 740

HSP 4 Score: 70.0922 bits (170), Expect = 6.017e-11
Identity = 70/267 (26.22%), Postives = 118/267 (44.19%), Query Frame = 3

Query: 1629 ELWKNK-------DKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPI-----HTQFENADYEKYRPLDQWWMKLRPLLRIIQTHE 2354
            EL KNK       +K+++  I  V +  +I N+  G    +G+DT ++ I+  +D +   +   Q R +I E +G +CGYL  +G LA  AD  +I E             ++    S  Q +NI+       +  G + R     HP  +  + K+  +  K   D R   +GHV  GG  S+FDR L  R G EA +    ++E   +   V++ L  + +  V P+      TQ       + R  D   ++ R   R ++T++
Sbjct:  140 ELVKNKTITTEQQEKFNVLHI--VGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQ-RTFIMEVMGRHCGYLALVGGLACEADFIFIPEMPPKVDWPDRLCSQLAQERSAGQRLNIVI------VAEGAMDREG---HPITAEDVKKVIDERLK--HDARITVLGHVQRGGNPSAFDRILACRMGAEATL---ALMEATKDSVPVVISLDGNQAVRV-PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYK 388

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Match: sp|Q01813|PFKAP_HUMAN (ATP-dependent 6-phosphofructokinase, platelet type OS=Homo sapiens OX=9606 GN=PFKP PE=1 SV=2)

HSP 1 Score: 488.419 bits (1256), Expect = 0.000e+0
Identity = 262/519 (50.48%), Postives = 352/519 (67.82%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            KF+      G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QG++DGG NI EA W  VS ++ +GGT+IGSARC  F+TR GRLKAA NL+ R I NL +IGGDGSLTGAN+F++EW+  ++EL     ID    +    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP + WE+ +C KL E ++  +R+N++I++EGAID  N+PIT++ IK++++ ++  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N  VR PLM+CV  T D+Q  M    F D V +RG SF  N   Y+ L + +P          +I K            T+  + ++  G  A GMNA +RS VR+ IA+    + I  GFDG  K  I  + W DV G    GGSI+GT RVLP +   E +A+++    ++AL+IIGGFE
Sbjct:   15 KFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEA--TPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDD--------QIPK------------TNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKY-LEEIATQMRTHSINALLIIGGFE 510

HSP 2 Score: 206.068 bits (523), Expect = 0.000e+0
Identity = 105/258 (40.70%), Postives = 162/258 (62.79%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY--------DNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   ++K++ F +P+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+    A+ W+   L+           +DSI +LG+S+    NVI  P+    +  D+E   P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  517 ELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKR---NVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVS 771

HSP 3 Score: 112.464 bits (280), Expect = 4.207e-24
Identity = 98/353 (27.76%), Postives = 168/353 (47.59%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA---IHALIDAAQ--------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++AV+  G  + GMNAAV+S VR+G+  G  +  + +G  G   G   I+E  W+DV G    GG+++G+ R L  K      + A  +    I  L+IIGG  +  G              L+E        +++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +        + L E   +  +  LV+ +E   + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A A   I A +  A+        + SI  CV+ I K  ++ +P+ +   +T
Sbjct:  412 NVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKG--QIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLE---EIATQMRTHSINALLIIGGFEAYLG--------------LLELSAAREKHEEFC--VPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSI--CVLGISKRNVIFQPVAELKKQT 739

HSP 4 Score: 66.2402 bits (160), Expect = 6.005e-15
Identity = 48/169 (28.40%), Postives = 83/169 (49.11%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKL-FKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            VV +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  ++ E         Q    L      + +  +++      +  + P  S  I +L   Q G   +D R   +GHV  GG  S+FDR L +R G+EA+I
Sbjct:  167 VVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLG---YDTRVTILGHVQRGGTPSAFDRILASRMGVEAVI 331

HSP 5 Score: 37.7354 bits (86), Expect = 6.005e-15
Identity = 43/127 (33.86%), Postives = 61/127 (48.03%), Query Frame = 2

Query: 1247 MVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            M  + +R  K S RK     S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I    W  V  I+ +GG+IIG+ R     ++ G    A  LL+  ++ L +IGG
Sbjct:    1 MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGG 127

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Match: sp|Q5R636|PFKAP_PONAB (ATP-dependent 6-phosphofructokinase, platelet type OS=Pongo abelii OX=9601 GN=PFKP PE=2 SV=1)

HSP 1 Score: 484.182 bits (1245), Expect = 0.000e+0
Identity = 261/519 (50.29%), Postives = 351/519 (67.63%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            KF+      G +I VLTSGGD+QGMNAAV++VVRMG+Y GA V F+ EG QG++DGG NI EA W  VS ++ +GGT+IGSARC  F+TR GRLKAA NL+ R I NL +IGGDGSLTGAN+F++EW+  ++EL     ID    +    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP + WE+ +C KL E ++  +R+N++I++EGAID  N+PIT++ IK++++ ++  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N  VR PLM+CV  T D+Q  M    F D V +RG SF  N   Y+ L + +P          +I K            T+  + ++  G  A GMNA +RS VR+ IA+    + I  GFDG  K  I  + W DV G    GGSI+GT RVLP +   E +A+++    ++AL+IIGGFE
Sbjct:   15 KFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVCFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEA--TPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDD--------QIPK------------TNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKY-LEEIATQMRTHSINALLIIGGFE 510

HSP 2 Score: 205.297 bits (521), Expect = 0.000e+0
Identity = 104/258 (40.31%), Postives = 161/258 (62.40%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN--------YDNDSIVILGLSQDTSANVI--PIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   ++K++ F +P+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L   V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+    A+ W+   L+           +DS+ +LG+S+    NVI  P+    +  D+E   P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  517 ELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSSVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKETRGRGKKFTTDDSVCVLGISKR---NVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVS 771

HSP 3 Score: 112.079 bits (279), Expect = 4.356e-24
Identity = 93/351 (26.50%), Postives = 163/351 (46.44%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ---------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++AV+  G  + GMNAAV+S VR+G+  G  +  + +G  G   G   I+E  W+DV G    GG+++G+ R L  K      + A  +    I  L+IIGG  +  G              L+E        +++C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +        + L E   +  +  LV+ +E   + +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A A+  +    +               CV+ I K  ++ +P+ +   +T
Sbjct:  412 NVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKG--QIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLE---EIATQMRTHSINALLIIGGFEAYLG--------------LLELSAAREKHEEFC--VPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSSVEHLTEKMKTTIQRGLVLRNESCSENYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKETRGRGKKFTTDDSVCVLGISKRNVIFQPVAELKKQT 739

HSP 4 Score: 66.2402 bits (160), Expect = 6.958e-15
Identity = 48/169 (28.40%), Postives = 83/169 (49.11%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKL-FKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAII 2165
            VV +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  ++ E         Q    L      + +  +++      +  + P  S  I +L   Q G   +D R   +GHV  GG  S+FDR L +R G+EA+I
Sbjct:  167 VVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLG---YDTRVTILGHVQRGGTPSAFDRILASRMGVEAVI 331

HSP 5 Score: 37.7354 bits (86), Expect = 6.958e-15
Identity = 43/127 (33.86%), Postives = 61/127 (48.03%), Query Frame = 2

Query: 1247 MVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRXXXXXXXXXXXTTR--VLPSQIGFEVVASRLLEFKLSALIIIGG 1615
            M  + +R  K S RK     S     IG++ SG  A GMNA +R+VVRM I        I  G+ G+      I    W  V  I+ +GG+IIG+ R     ++ G    A  LL+  ++ L +IGG
Sbjct:    1 MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVCFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGG 127

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Match: sp|P47860|PFKAP_RAT (ATP-dependent 6-phosphofructokinase, platelet type OS=Rattus norvegicus OX=10116 GN=Pfkp PE=1 SV=2)

HSP 1 Score: 478.404 bits (1230), Expect = 0.000e+0
Identity = 259/510 (50.78%), Postives = 346/510 (67.84%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QG++DGG NI EA W  VS ++ +GGT+IGSARC  F++R GRLKAA NL+   I NL +IGGDGSLTGAN+F++EW+  ++EL +   IDS   K    LN+VG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VF+PE PP + WE+++C KL E ++  +R+N++I+SEGAID  N+PIT++ IK++++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+Q  M    F + V +RG SF  N   Y+ L +  P          +I K            ++  + I+  G  A GMNA +RS VR+ IA       I  GFDGL    I  + W DV G    GGSI+GT R LP +   E +A ++    ++AL+IIGGFE
Sbjct:   24 GKAIGVLTSGGDAQGMNAAVRAVVRMGIYTGAKVYFIYEGYQGMVDGGSNIVEAKWECVSSILQVGGTIIGSARCQAFRSREGRLKAACNLVRLGITNLCVIGGDGSLTGANLFRKEWSGLLEELAKNGEIDSDTVKKHAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEEMCLKLSENRARKKRLNIIIVSEGAIDTQNKPITSEKIKELVVTNLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEA--TPETPACVVSLRGNQAVRLPLMECVQMTQDVQKAMDERRFDEAVKLRGRSFEGNLNTYKRLAIKEPDD--------KIPK------------SNCNVAIINVGAPAAGMNAAVRSAVRVGIAEGHKMFAIYDGFDGLANGQIKEIGWGDVGGWTGQGGSILGTKRTLPGKY-LEKIAEQMHSKNINALLIIGGFE 510

HSP 2 Score: 200.675 bits (509), Expect = 0.000e+0
Identity = 102/256 (39.84%), Postives = 158/256 (61.72%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLEN--------YDNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   ++K++ F +P+V++PAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL  MG LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN      Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+   L+           +DSI +LG+ Q       P+    +  D+E   P  QWW+KLRP+++I+  +E+ Y+ S
Sbjct:  517 ELAAARNKHEAFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEQFDIRDLQSNVMHLTEKMKTSIQRGLVLRNENCSVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSHGTGKKFVSDDSICVLGI-QKRDLLFKPVAELRKATDFEHRIPKQQWWLKLRPIMKILAKYEASYDMS 771

HSP 3 Score: 114.005 bits (284), Expect = 1.101e-24
Identity = 102/359 (28.41%), Postives = 180/359 (50.14%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEA---IHALIDAAQ--------NPSIPACVICIVKNEIVRKPLMDCVSKTLDIQN 1135
            ++A++  G  + GMNAAV+S VR+G+  G  ++ + +G  GL +G   I+E  W DV G    GG+++G+ R L  K      K A+ +  + I  L+IIGG  +  G              L+E     +  + +C  + +V +  ++ N+  G+D +IG ++AL  I +  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+  +  + + ++    E+ K+S +R  LV+ +E      N      Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A   I A +  +         + SI  CV+ I K +++ KP+ + + K  D ++
Sbjct:  412 NVAIINVGAPAAGMNAAVRSAVRVGIAEGHKMFAIYDGFDGLANG--QIKEIGWGDVGGWTGQGGSILGTKRTLPGKYLE---KIAEQMHSKNINALLIIGGFEAYLG--------------LLELAAARNKHEAFC--VPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEQFDIRDLQSNVMHLTEKMKTSIQR-GLVLRNENC--SVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKLKGSHGTGKKFVSDDSI--CVLGIQKRDLLFKPVAE-LRKATDFEH 743

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Match: G4VKA5 (ATP-dependent 6-phosphofructokinase OS=Schistosoma mansoni OX=6183 GN=Smp_043670.2 PE=3 SV=1)

HSP 1 Score: 535.798 bits (1379), Expect = 0.000e+0
Identity = 280/525 (53.33%), Postives = 373/525 (71.05%), Query Frame = 2

Query:   47 LKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            L   EGK++  G F G  IAVLTSGGD+QGMNAAV++VVRMG+YCG  V+F+ EG QGL+DGG NI+EA W+DVSG++ LGGT IGSARC+DF+ R GRLKAA+NL+  QI NLV+IGGDGSLTGAN+F+ EW+S ++EL+    I +   K    LNIVGLVGSIDNDFCGTDMTIG +SAL RI+EATDA+ TTAHSHQRCF++EVMGRHCGYLAL+ +++ EA +VFIPE PP D+W + LC KL   +   +RVN+++++EGAID   +PIT + +K++I+  +++DTRITVLGHVQRGG+P++FDRIL  R GAEA+ AL+DA ++P++P+CVI +  N+ VR PL+ CV +T  +   M+   F   V +RG SF  N   Y                  ++SK  + ++ + +S  + RIGI+  G  ACG+NA+IR   R+ I      IGI  GF GL K   + + W DVRG + MGGS++GT R  P+ +G + VA++  E KLS L+IIGGFE
Sbjct:   18 LPGMEGKYVARGQFTGECIAVLTSGGDAQGMNAAVRAVVRMGIYCGCRVFFIREGYQGLVDGGQNIQEASWADVSGILQLGGTKIGSARCMDFRERYGRLKAAENLVKNQITNLVVIGGDGSLTGANLFRAEWSSLLEELVTSNKISAESAKQFHRLNIVGLVGSIDNDFCGTDMTIGADSALHRIIEATDAISTTAHSHQRCFILEVMGRHCGYLALVASMACEADWVFIPEMPPTDDWREKLCHKLRMNREHGQRVNIIMVAEGAIDRACKPITCEIVKNLIVSELQLDTRITVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDADRDPNLPSCVISLDGNQAVRVPLVKCVDRTRQVAEAMKACDFDHAVELRGTSFMNNLATY-----------------IKLSKIEQPRQSVMSSENNLRIGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKGDASEIQWADVRGWVGMGGSMLGTRRDTPNGLGIDKVAAKFKELKLSGLLIIGGFE 525

HSP 2 Score: 221.476 bits (563), Expect = 0.000e+0
Identity = 111/263 (42.21%), Postives = 169/263 (64.26%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDNDSIV---------ILGL--SQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            EL + ++KY    IP+ ++PATI+NN+PG  F++G DT +N I + LD +K  ++GT+ R+++ ET+G  CGYL TM ALAGGAD +YI EE   ++ L +DV  L+AK+ D +K GLVLR   ++  Y S +I +L+ QEG+GIFDCR N +GH+  G   S FDR+LGT+   +AI W+ + +  N  +DS V         ++G+   Q T +N++ +    E+ D+    P ++WW+ LRPL+RI+  H+S YE+  IM
Sbjct:  532 ELVEGREKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVVHLRAKIDDNVKRGLVLRAEYANKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWLDEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELR---EHTDFVHRLPKEEWWLSLRPLMRIMAKHDSLYESESIM 791

HSP 3 Score: 110.923 bits (276), Expect = 3.498e-21
Identity = 111/400 (27.75%), Postives = 193/400 (48.25%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLV-GSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXX--XXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSI--------PACVIC----IVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDL-VMMRGPSFYRNYKIYEALNVLMPKHRK 1246
            I ++  G  + G+NA ++   R+G+  G  V  + EG  GL+ G  +  E  W+DV G V +GG+++G+ R  D     G  K A    + ++  L+IIGG            E    + EL+E +      +KY +    + +V  +I N+  GTD ++G ++AL  I    D +  +A  + +R FV+E MG +CGYLA ++A++  A   +I E+P   D+  +D+   L      N +  LV+ +E A    N+  T+++I  +     +   D R  VLGH+Q+G  P+ FDR L  +  ++AI  L D   N +I        P  + C    IV+ +     +M+    T  +  L +   ++ L  +MR     ++  +YE+ +++    RK
Sbjct:  427 IGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKG--DASEIQWADVRGWVGMGGSMLGTRR--DTPNGLGIDKVAAKFKELKLSGLLIIGG-----------FEAYECMIELVEGR------EKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVV-HLRAKIDDNVKRGLVLRAEYA----NKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWL-DEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELREHTDFVHRLPKEEWWLSLRPLMR--IMAKHDSLYESESIMAGTDRK 797

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Match: G4VKA6 (ATP-dependent 6-phosphofructokinase OS=Schistosoma mansoni OX=6183 GN=Smp_043670.1 PE=3 SV=1)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 280/525 (53.33%), Postives = 373/525 (71.05%), Query Frame = 2

Query:   47 LKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            L   EGK++  G F G  IAVLTSGGD+QGMNAAV++VVRMG+YCG  V+F+ EG QGL+DGG NI+EA W+DVSG++ LGGT IGSARC+DF+ R GRLKAA+NL+  QI NLV+IGGDGSLTGAN+F+ EW+S ++EL+    I +   K    LNIVGLVGSIDNDFCGTDMTIG +SAL RI+EATDA+ TTAHSHQRCF++EVMGRHCGYLAL+ +++ EA +VFIPE PP D+W + LC KL   +   +RVN+++++EGAID   +PIT + +K++I+  +++DTRITVLGHVQRGG+P++FDRIL  R GAEA+ AL+DA ++P++P+CVI +  N+ VR PL+ CV +T  +   M+   F   V +RG SF  N   Y                  ++SK  + ++ + +S  + RIGI+  G  ACG+NA+IR   R+ I      IGI  GF GL K   + + W DVRG + MGGS++GT R  P+ +G + VA++  E KLS L+IIGGFE
Sbjct:   14 LPGMEGKYVARGQFTGECIAVLTSGGDAQGMNAAVRAVVRMGIYCGCRVFFIREGYQGLVDGGQNIQEASWADVSGILQLGGTKIGSARCMDFRERYGRLKAAENLVKNQITNLVVIGGDGSLTGANLFRAEWSSLLEELVTSNKISAESAKQFHRLNIVGLVGSIDNDFCGTDMTIGADSALHRIIEATDAISTTAHSHQRCFILEVMGRHCGYLALVASMACEADWVFIPEMPPTDDWREKLCHKLRMNREHGQRVNIIMVAEGAIDRACKPITCEIVKNLIVSELQLDTRITVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDADRDPNLPSCVISLDGNQAVRVPLVKCVDRTRQVAEAMKACDFDHAVELRGTSFMNNLATY-----------------IKLSKIEQPRQSVMSSENNLRIGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKGDASEIQWADVRGWVGMGGSMLGTRRDTPNGLGIDKVAAKFKELKLSGLLIIGGFE 521

HSP 2 Score: 221.861 bits (564), Expect = 0.000e+0
Identity = 111/263 (42.21%), Postives = 169/263 (64.26%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDNDSIV---------ILGL--SQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            EL + ++KY    IP+ ++PATI+NN+PG  F++G DT +N I + LD +K  ++GT+ R+++ ET+G  CGYL TM ALAGGAD +YI EE   ++ L +DV  L+AK+ D +K GLVLR   ++  Y S +I +L+ QEG+GIFDCR N +GH+  G   S FDR+LGT+   +AI W+ + +  N  +DS V         ++G+   Q T +N++ +    E+ D+    P ++WW+ LRPL+RI+  H+S YE+  IM
Sbjct:  528 ELVEGREKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVVHLRAKIDDNVKRGLVLRAEYANKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWLDEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELR---EHTDFVHRLPKEEWWLSLRPLMRIMAKHDSLYESESIM 787

HSP 3 Score: 110.923 bits (276), Expect = 3.596e-21
Identity = 111/400 (27.75%), Postives = 193/400 (48.25%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLV-GSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXX--XXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSI--------PACVIC----IVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDL-VMMRGPSFYRNYKIYEALNVLMPKHRK 1246
            I ++  G  + G+NA ++   R+G+  G  V  + EG  GL+ G  +  E  W+DV G V +GG+++G+ R  D     G  K A    + ++  L+IIGG            E    + EL+E +      +KY +    + +V  +I N+  GTD ++G ++AL  I    D +  +A  + +R FV+E MG +CGYLA ++A++  A   +I E+P   D+  +D+   L      N +  LV+ +E A    N+  T+++I  +     +   D R  VLGH+Q+G  P+ FDR L  +  ++AI  L D   N +I        P  + C    IV+ +     +M+    T  +  L +   ++ L  +MR     ++  +YE+ +++    RK
Sbjct:  423 IGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKG--DASEIQWADVRGWVGMGGSMLGTRR--DTPNGLGIDKVAAKFKELKLSGLLIIGG-----------FEAYECMIELVEGR------EKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVV-HLRAKIDDNVKRGLVLRAEYA----NKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWL-DEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELREHTDFVHRLPKEEWWLSLRPLMR--IMAKHDSLYESESIMAGTDRK 793

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Match: A0A3Q0KF43 (ATP-dependent 6-phosphofructokinase OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 532.332 bits (1370), Expect = 0.000e+0
Identity = 280/525 (53.33%), Postives = 373/525 (71.05%), Query Frame = 2

Query:   47 LKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            L   EGK++  G F G  IAVLTSGGD+QGMNAAV++VVRMG+YCG  V+F+ EG QGL+DGG NI+EA W+DVSG++ LGGT IGSARC+DF+ R GRLKAA+NL+  QI NLV+IGGDGSLTGAN+F+ EW+S ++EL+    I +   K    LNIVGLVGSIDNDFCGTDMTIG +SAL RI+EATDA+ TTAHSHQRCF++EVMGRHCGYLAL+ +++ EA +VFIPE PP D+W + LC KL   +   +RVN+++++EGAID   +PIT + +K++I+  +++DTRITVLGHVQRGG+P++FDRIL  R GAEA+ AL+DA ++P++P+CVI +  N+ VR PL+ CV +T  +   M+   F   V +RG SF  N   Y                  ++SK  + ++ + N++   RIGI+  G  ACG+NA+IR   R+ I      IGI  GF GL K   + + W DVRG + MGGS++GT R  P+ +G + VA++  E KLS L+IIGGFE
Sbjct:   14 LPGMEGKYVARGQFTGECIAVLTSGGDAQGMNAAVRAVVRMGIYCGCRVFFIREGYQGLVDGGQNIQEASWADVSGILQLGGTKIGSARCMDFRERYGRLKAAENLVKNQITNLVVIGGDGSLTGANLFRAEWSSLLEELVTSNKISAESAKQFHRLNIVGLVGSIDNDFCGTDMTIGADSALHRIIEATDAISTTAHSHQRCFILEVMGRHCGYLALVASMACEADWVFIPEMPPTDDWREKLCHKLRMNREHGQRVNIIMVAEGAIDRACKPITCEIVKNLIVSELQLDTRITVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDADRDPNLPSCVISLDGNQAVRVPLVKCVDRTRQVAEAMKACDFDHAVELRGTSFMNNLATY-----------------IKLSKIEQPRQSVMNNL---RIGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKGDASEIQWADVRGWVGMGGSMLGTRRDTPNGLGIDKVAAKFKELKLSGLLIIGGFE 518

HSP 2 Score: 221.476 bits (563), Expect = 0.000e+0
Identity = 111/263 (42.21%), Postives = 169/263 (64.26%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDNDSIV---------ILGL--SQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            EL + ++KY    IP+ ++PATI+NN+PG  F++G DT +N I + LD +K  ++GT+ R+++ ET+G  CGYL TM ALAGGAD +YI EE   ++ L +DV  L+AK+ D +K GLVLR   ++  Y S +I +L+ QEG+GIFDCR N +GH+  G   S FDR+LGT+   +AI W+ + +  N  +DS V         ++G+   Q T +N++ +    E+ D+    P ++WW+ LRPL+RI+  H+S YE+  IM
Sbjct:  525 ELVEGREKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVVHLRAKIDDNVKRGLVLRAEYANKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWLDEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELR---EHTDFVHRLPKEEWWLSLRPLMRIMAKHDSLYESESIM 784

HSP 3 Score: 110.923 bits (276), Expect = 3.086e-21
Identity = 111/400 (27.75%), Postives = 193/400 (48.25%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLV-GSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXX--XXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSI--------PACVIC----IVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDL-VMMRGPSFYRNYKIYEALNVLMPKHRK 1246
            I ++  G  + G+NA ++   R+G+  G  V  + EG  GL+ G  +  E  W+DV G V +GG+++G+ R  D     G  K A    + ++  L+IIGG            E    + EL+E +      +KY +    + +V  +I N+  GTD ++G ++AL  I    D +  +A  + +R FV+E MG +CGYLA ++A++  A   +I E+P   D+  +D+   L      N +  LV+ +E A    N+  T+++I  +     +   D R  VLGH+Q+G  P+ FDR L  +  ++AI  L D   N +I        P  + C    IV+ +     +M+    T  +  L +   ++ L  +MR     ++  +YE+ +++    RK
Sbjct:  420 IGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSGLVKG--DASEIQWADVRGWVGMGGSMLGTRR--DTPNGLGIDKVAAKFKELKLSGLLIIGG-----------FEAYECMIELVEGR------EKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIKQSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVV-HLRAKIDDNVKRGLVLRAEYA----NKYYTSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDRSLGTKFASKAIDWL-DEQINANIRSDSTVYTPGHLCCTLIGIVRRQTTYSNIMELREHTDFVHRLPKEEWWLSLRPLMR--IMAKHDSLYESESIMAGTDRK 790

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Match: A0A158QJ34 (ATP-dependent 6-phosphofructokinase OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS10809 PE=3 SV=1)

HSP 1 Score: 524.628 bits (1350), Expect = 0.000e+0
Identity = 272/521 (52.21%), Postives = 369/521 (70.83%), Query Frame = 2

Query:   59 EGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            EGK++  G F G SIAVL+SGGD+QGMNAA+++VVRMG+YCG  VYFV EG QGL+DGG NI+EA W+DVSG+  LGGTVIGSARC DF+ RSGR KAA N++   I NLV+IGGDGSLTGAN F+ EW+S ++ L+E   I S ++   ++LNIVG+VGSIDNDFCGTDMTIG +SAL R++EA DA++TTA SHQRCF++EVMGR+CGYLAL++A++SEA +VFIPE PP +NW + LC K+   +   +RV++++++EGA+D  ++PIT   +K++I +++ +DTR+TVLGHVQRGG P++FDR+L +R GAEA+ AL+DA + P +PACVI +V N+ VR PLM CV KT  +   +    F   V +RG SF  N   Y  L+ + P                 R +         R+G++  G  A G+NA +RS  R+AI      +GI  GF GL    +  +LW+DVRG +S GGS +GT R  P ++G ++VA+RL EFK+S L++IGGFE
Sbjct:   23 EGKYVAKGQFSGKSIAVLSSGGDAQGMNAAIRAVVRMGIYCGCRVYFVKEGYQGLVDGGNNIKEASWADVSGISQLGGTVIGSARCKDFRHRSGREKAALNMVKHNIHNLVVIGGDGSLTGANEFRVEWSSLLNSLVETGAITSEERTNHEHLNIVGIVGSIDNDFCGTDMTIGADSALHRVIEAADAIVTTAQSHQRCFILEVMGRNCGYLALLSALASEADWVFIPEVPPAENWPELLCSKVSRNRERGQRVSIIMVAEGALDRESKPITCDMVKNLITEKLGLDTRVTVLGHVQRGGRPSAFDRVLGIRMGAEAVLALMDADKKPDLPACVITLVGNQAVRLPLMHCVEKTRSVAAAIAKRDFEQAVFLRGRSFMSNVDTYLTLSKINP------------PAVENRNKL--------RVGVVNVGAPAGGVNAAMRSFTRIAITKGYRVMGIFEGFQGLVNGEVKDILWDDVRGWVSQGGSNLGTRRPTPKELGLDLVAARLAEFKISGLMVIGGFE 523

HSP 2 Score: 194.512 bits (493), Expect = 0.000e+0
Identity = 103/265 (38.87%), Postives = 165/265 (62.26%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY----------DNDSIVILGLSQDTSANVIPIHTQFEN----ADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            +L + + KY    IP++++PATI+NN+PG  F++G+DT +N I   +D +K  + G++ R+++ ET+G  CGYL T+GA+AGGAD +YI EE   ++ L +DV  L+AK+   ++ GLVLR   ++  Y + +I KLF +EG+GIFDCR   +GH+  GG  S FDR  GT+ G+ AI W+   +  +          ++++ V+LGL +         +++ E+     D++   P+D WW+ LRPL+RI+  HES YE+  IM
Sbjct:  530 DLAEGRSKYKELCIPMIMLPATISNNVPGTDFSLGADTALNEITVAIDKIKQSATGSKRRVFVVETMGGYCGYLATIGAVAGGADAAYIHEEAFGIDDLREDVTHLRAKMASNVQRGLVLRAEYANKNYTTDFIHKLFTEEGQGIFDCRMCVLGHIQQGGEPSPFDRIHGTKLGVLAIDWLDTQMNTFLTQEGSVCTSNHEACVLLGLIKRAYE-----YSRVEDLKRITDFKHRVPVDNWWLNLRPLMRIMAKHESLYESESIM 789

HSP 3 Score: 109.768 bits (273), Expect = 6.981e-21
Identity = 99/311 (31.83%), Postives = 160/311 (51.45%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXX--XXXVLGHVQRGGNPTSFDRILALREGAEAIHAL 1021
            + V+  G  + G+NAA++S  R+ +  G  V  + EG QGL++G   +++  W DV G V+ GG+ +G+ R        G    A  L + +I  L++IGG           + +   ID L E +   S  K+ C  + ++ L  +I N+  GTD ++G ++AL  I  A D +  +A  S +R FV+E MG +CGYLA I A++  A   +I E+    D+  +D+   L    +SN +  LV+ +E A    N+  T  +I  +  +  +     R+ VLGH+Q+GG P+ FDRI   + G  AI  L
Sbjct:  425 VGVVNVGAPAGGVNAAMRSFTRIAITKGYRVMGIFEGFQGLVNG--EVKDILWDDVRGWVSQGGSNLGTRR--PTPKELGLDLVAARLAEFKISGLMVIGG----------FEAFECMID-LAEGR---SKYKELC--IPMIMLPATISNNVPGTDFSLGADTALNEITVAIDKIKQSATGSKRRVFVVETMGGYCGYLATIGAVAGGADAAYIHEEAFGIDDLREDV-THLRAKMASNVQRGLVLRAEYA----NKNYTTDFIHKLFTEEGQGIFDCRMCVLGHIQQGGEPSPFDRIHGTKLGVLAIDWL 710

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BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Match: A0A0B6Z7M5 (ATP-dependent 6-phosphofructokinase OS=Arion vulgaris OX=1028688 GN=ORF52305 PE=3 SV=1)

HSP 1 Score: 523.087 bits (1346), Expect = 0.000e+0
Identity = 280/522 (53.64%), Postives = 360/522 (68.97%), Query Frame = 2

Query:   56 KEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            KEG+ +P G + G+SI V TSGGDSQGMNAAV++VVRMG Y G  VYF+ EG QG++DGG+NI EA WS VSG++ +GGT+IGSARC DF+ R GRL AA NL+  QI NLV+IGGDGSLTGA+ F+QEW+  +DEL++ K I   ++  CKNLNIVGLVGSIDNDFCGTDMTIG +SAL RI+EA DA+ TTA SHQR FV+EVMGRHCGYLAL+ A+++EA +VFIPE PP  +W+D LCKKL   +   +R+N+++++EGAID    PITA+ +K ++ DR+K+DTR+TVLGHVQRGG+P++FDRIL  R GAEA+ AL+DA   P  PACVI IV N  VR PL++CV +T  +Q  M    F + V +RG SF  N   Y  L+ L P           I K S  K           I IM  G+ ACGMNA  RS VR+A+      +GI   FDGL    +TP+ W  V+G    GGS++GT +     +G   +A +  E+KL  L+I+GGF+
Sbjct:   25 KEGQLVPPGIWAGNSIGVFTSGGDSQGMNAAVRAVVRMGFYLGCKVYFIKEGYQGMVDGGINIVEATWSSVSGILQMGGTIIGSARCKDFRERKGRLTAANNLVQYQITNLVVIGGDGSLTGADCFRQEWSGLLDELLQNKSITEQQRANCKNLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEAVDAIATTALSHQRAFVLEVMGRHCGYLALVGALATEASWVFIPEWPPGGDWQDKLCKKLSAERQLLQRLNIILVAEGAIDDTGNPITAEAVKQLLSDRLKMDTRVTVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDA--TPETPACVISIVGNSTVRVPLVECVQRTKAVQAAMDARNFEEAVRLRGKSFQNNLNTYRLLSKLRPPS---------IIKNSTDK-------PQHNIAIMNLGSPACGMNAAARSFVRVALTKGYNVLGINDSFDGLLSGNVTPMTWTKVQGWSGTGGSLLGTQKQSAQDVGIGKIALKFSEYKLDGLMIVGGFQ 528

HSP 2 Score: 204.527 bits (519), Expect = 0.000e+0
Identity = 106/270 (39.26%), Postives = 158/270 (58.52%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDE-IKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY----------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTH-ESQYETSFIMRHL 2390
            LS  +L   ++ +    IP+V IP TI+NN+PG   ++G+DT +N I +  D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI+EE   +  L QDV  LK K+ +  +  GLVLRN +++  Y + +I +LF +EG  +F  R N +GHV  GG  S FDRN GT+   + + W+ +M+  +          + D++V+LG+ +       P+    E  DY K  P +QWW+KLRPLLRI+  H E  YET   M ++
Sbjct:  531 LSACQLADAREMFPSLCIPIVAIPCTISNNVPGSDISLGADTAINEITDICDRIKQSATGTKRRVFIAETMGGYCGYLATMAGLASGADAAYINEEKFGVIDLKQDVEHLKDKILNAGVLRGLVLRNEMANENYTTEFINQLFSEEGYPVFTTRMNILGHVQQGGYPSPFDRNYGTKLAAKGVEWMSEMIATFSTPNGPVRTGNADNVVLLGMLK-RHLTFTPVQELAEATDYVKRIPKEQWWLKLRPLLRILAKHSEVNYETVAEMANI 799

HSP 3 Score: 96.6709 bits (239), Expect = 9.010e-17
Identity = 90/332 (27.11%), Postives = 158/332 (47.59%), Query Frame = 2

Query:   95 HSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAI-------DMHNQPITAQYXXXXXXXX--XXXXXXXXVLGHVQRGGNPTSFDRILALR---EGAEAIHALIDAAQNPSIP 1051
            H+IA++  G  + GMNAA +S VR+ L  G  V  + +   GL+ G  N+    W+ V G    GG+++G+ +        G  K A    + ++  L+I+GG           Q + S       R++  S+    C  + IV +  +I N+  G+D+++G ++A+  I +  D +  +A  + +R F+ E MG +CGYLA +  ++S A   +I E+        DL + +E  K         I++ G +       +M N+  T ++I  +  +       TR+ +LGHVQ+GG P+ FDR    +   +G E +  +I     P+ P
Sbjct:  428 HNIAIMNLGSPACGMNAAARSFVRVALTKGYNVLGINDSFDGLLSG--NVTPMTWTKVQGWSGTGGSLLGTQK--QSAQDVGIGKIALKFSEYKLDGLMIVGG----------FQAFLSACQLADAREMFPSL----C--IPIVAIPCTISNNVPGSDISLGADTAINEITDICDRIKQSATGTKRRVFIAETMGGYCGYLATMAGLASGADAAYINEEKFG---VIDLKQDVEHLKDK-------ILNAGVLRGLVLRNEMANENYTTEFINQLFSEEGYPVFTTRMNILGHVQQGGYPSPFDRNYGTKLAAKGVEWMSEMIATFSTPNGP 729

HSP 4 Score: 70.8626 bits (172), Expect = 9.172e-9
Identity = 57/208 (27.40%), Postives = 99/208 (47.60%), Query Frame = 3

Query: 1629 ELWKNKD-----KYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKA--KLFDEIKYGLVLRNNV--SHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLS 2225
            EL +NK      + +   + +V +  +I N+  G    +G+D+ ++ I+  +D +   ++  Q R ++ E +G +CGYL  +GALA  A   +I E     +  ++    L A  +L   +   LV    +  + +P  +  + +L     K   D R   +GHV  GG  S+FDR LG+R G EA++ + D         I I+G S
Sbjct:  160 ELLQNKSITEQQRANCKNLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEAVDAIATTALSHQ-RAFVLEVMGRHCGYLALVGALATEASWVFIPEWPPGGDWQDKLCKKLSAERQLLQRLNIILVAEGAIDDTGNPITAEAVKQLLSDRLK--MDTRVTVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDATPETPACVISIVGNS 364

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Match: pfklb (ATP-dependent 6-phosphofructokinase, liver type [Source:NCBI gene;Acc:103036959])

HSP 1 Score: 483.026 bits (1242), Expect = 0.000e+0
Identity = 257/511 (50.29%), Postives = 347/511 (67.91%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELI-ERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++V RMG+Y GA VY + EG QGL+DGG NI+ A W  V+ ++ LGGTVIGSARC  F TR GRL AA +L+ R I NL + GGDGSLTGANIF+ EW   + +L+ E ++ D++ ++Y  +LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLA+++A++S A +VFIPE PP + WED +C +L E++S   R+N+VII+EGAID H QPIT+ Y+KD+++ R+  DTR+TVLGHVQRGG P++FDR+L+ + G EA+ AL++A   P  PACVI +  N+ +R PLM+CV+ T ++Q  M    F + + +RG SF  N+  Y+ L    P   K                      ++  + I+  G  A GMNA +RS VR+ +A  L    +  GF+GL K  +T + W+DV G    GGS++GT R LP+    E +   L ++ + +L+ IGGFE
Sbjct:   14 GKAIAVLTSGGDAQGMNAAVRAVTRMGIYVGAKVYLIYEGYQGLVDGGENIKIANWQSVTNIIQLGGTVIGSARCKAFTTREGRLSAAFHLVQRGITNLCVCGGDGSLTGANIFRSEWAELLAQLVKEGRITDTLAQQYT-HLNIVGLVGSIDNDFCGTDMTIGADSALHRIMEVIDAISTTAQSHQRTFVLEVMGRHCGYLAVVSALASGADWVFIPEAPPEEGWEDHMCARLGESRSKGSRLNIVIIAEGAIDKHGQPITSNYVKDLVVQRLGYDTRVTVLGHVQRGGTPSAFDRVLSSKLGVEAVVALLEA--TPETPACVIGLSGNQAMRLPLMECVNMTKEVQKAMNEKRFDEAIQLRGKSFENNWNTYKLLAHQKPAQSK----------------------SEHSMAILNVGAPAAGMNAAVRSAVRVGLAQGLRVYTVNDGFEGLAKDMVTEVHWHDVAGWTGQGGSLLGTKRTLPNSC-MEKIVETLRKYNIQSLLAIGGFE 498

HSP 2 Score: 201.83 bits (512), Expect = 0.000e+0
Identity = 101/262 (38.55%), Postives = 159/262 (60.69%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKD-MLENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRII---QTHESQYETSFI 2378
            +L + + +YD   I + +IPATI+NN+PG  F++G+DT VN  + + D +K  + GT+ R++I ET+G  CGYL T   +A GAD +YI EE  N+  L  +V  L  K+  +I+ GLVLRN   H  Y + +I  L+  EGKGIFDCR N +GH+  GG+ + FDRN GT+ G++A+ W+ + M E +          ++  ++G+++    + IP+    E+ D+E   P +QWW+ LR +L+++   QTH  +Y T  I
Sbjct:  505 QLVEARGRYDELCIVMCVIPATISNNVPGTNFSLGADTAVNAAMESCDKIKQSASGTKRRVFIVETMGGYCGYLATTTGIAVGADAAYIFEEPFNIHDLEMNVEHLTEKMKKDIQRGLVLRNEKCHKHYTTDFIHNLYSAEGKGIFDCRVNILGHLQQGGVPTPFDRNFGTKMGVKAVQWLTEKMRETHRQGRVFANAPETACVIGMNRKV-MSFIPVAELKEHTDFEHRMPKEQWWINLRVMLKMLARYQTHFDEYVTGDI 765

HSP 3 Score: 123.635 bits (309), Expect = 1.211e-28
Identity = 108/389 (27.76%), Postives = 184/389 (47.30%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIE-RKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDL-VMMRGPSFYRNY 1201
            PA     HS+A+L  G  + GMNAAV+S VR+GL  G  VY V +G +GL      + E +W DV+G    GG+++G+ R L     S   K  + L    I +L+ IGG  +  G           + +L+E R   D +    C       +  +I N+  GT+ ++G ++A+   ME+ D +  +A  + +R F++E MG +CGYLA  T I+  A   +I E+P N +  +   + L E    + +  LV+ +E     +       +       +   D R+ +LGH+Q+GG PT FDR    + G +A+  L +  +          N    ACVI + +  +   P+ +    T     + +   +++L VM++  + Y+ +
Sbjct:  392 PAQSKSEHSMAILNVGAPAAGMNAAVRSAVRVGLAQGLRVYTVNDGFEGLAKD--MVTEVHWHDVAGWTGQGGSLLGTKRTL---PNSCMEKIVETLRKYNIQSLLAIGGFEAYEG-----------VLQLVEARGRYDELCIVMCV------IPATISNNVPGTNFSLGADTAVNAAMESCDKIKQSASGTKRRVFIVETMGGYCGYLATTTGIAVGADAAYIFEEPFNIHDLEMNVEHLTEKMKKDIQRGLVLRNEKCHKHYTTDFI--HNLYSAEGKGIFDCRVNILGHLQQGGVPTPFDRNFGTKMGVKAVQWLTEKMRETHRQGRVFANAPETACVIGMNRKVMSFIPVAELKEHTDFEHRMPKEQWWINLRVMLKMLARYQTH 756

HSP 4 Score: 71.2478 bits (173), Expect = 2.728e-12
Identity = 55/196 (28.06%), Postives = 97/196 (49.49%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYG------LVLRNNVSHH--PYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTS 2237
            +V +  +I N+  G    +G+D+ ++ I+  +D +   +   Q R ++ E +G +CGYL  + ALA GAD  +I E     E   +D   + A+L +    G      ++    +  H  P  S+Y+  L  Q  +  +D R   +GHV  GG  S+FDR L ++ G+EA++    +LE        ++GLS + +
Sbjct:  157 IVGLVGSIDNDFCGTDMTIGADSALHRIMEVIDAISTTAQSHQ-RTFVLEVMGRHCGYLAVVSALASGADWVFIPEA--PPEEGWED--HMCARLGESRSKGSRLNIVIIAEGAIDKHGQPITSNYVKDLVVQ--RLGYDTRVTVLGHVQRGGTPSAFDRVLSSKLGVEAVV---ALLEATPETPACVIGLSGNQA 342

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Match: pfkma (ATP-dependent 6-phosphofructokinase, muscle type-like [Source:NCBI gene;Acc:103038352])

HSP 1 Score: 479.559 bits (1233), Expect = 0.000e+0
Identity = 255/511 (49.90%), Postives = 345/511 (67.51%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEAL-NVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++ VR+GLY GA V+FV EG QGL+DGG NIR A W  VS M+ LGGTVIGSARC DF+TR GR+KAA NL+ R I NL +IGGDGSLTGAN+F+ EW+  + +L+ +  I   + K   +LNIVG+VGSIDNDFCGTDMTIG +SAL RIME  D++ TTA SHQR F++EVMGRHCGYLAL+TA++  A +VFIPE PP+D WE+ LC++L E ++   R+N++I++EGA+    +PIT   IK+++  R+  DTR T+LGHVQRGG P++FDRIL  R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+   M+ G + D V +RG SF  N+  Y+ L +V +P+ +                       ++  + I+  G    GMNA +R+ VR+ I      + +  GF+GL +  I P+ WN V G    GGS++GT RVLP++   E ++  + +  + AL+IIGGFE
Sbjct:   17 GRAIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVFFVHEGYQGLVDGGDNIRPATWESVSMMLQLGGTVIGSARCKDFQTREGRIKAAYNLVKRGITNLCVIGGDGSLTGANLFRTEWSGLLGDLVSKGKITGDEAKSASHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDSITTTAQSHQRAFILEVMGRHCGYLALVTALACGADWVFIPEMPPDDGWEEHLCRRLMETRNRGSRLNVIIVAEGAMGRDGKPITCDQIKELVSKRLGYDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEA--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTVAMKEGRYDDAVKLRGRSFENNWNTYKLLAHVHLPETK-----------------------SNINVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQGKIEPINWNSVGGWTGKGGSVLGTKRVLPAK-HIEEISLNIAKHNIHALVIIGGFE 501

HSP 2 Score: 208.379 bits (529), Expect = 0.000e+0
Identity = 104/264 (39.39%), Postives = 160/264 (60.61%), Query Frame = 3

Query: 1611 EALSGQ-ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EA SG  EL + + KY+   IP+V+IPAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI E+   +  L  +V  L  K+   +K GL+LRN   +  Y + +IF L+ +EGKGIFDCR N +GH+  GG  + FDRN  T+ G ++++W+ + L E Y +        DS  +LG+ +        +    E  D+E   P  +WW+KLRP+L+I+  ++   +T+
Sbjct:  501 EAFSGGLELVQARQKYEELCIPMVVIPATVSNNVPGSDFSIGADTALNTITSTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLAAGADAAYIYEDKFGIRDLEANVEHLVEKMKTTVKRGLILRNENCNANYTTDFIFNLYSEEGKGIFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKSVLWLTEKLKECYRHGRIFANSADSACVLGMKK-RGLMFQALAELKEETDFEHRIPKTEWWLKLRPILKILAKYKISLDTT 763

HSP 3 Score: 116.701 bits (291), Expect = 2.196e-26
Identity = 100/359 (27.86%), Postives = 170/359 (47.35%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPE-KPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXX------XXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++A+L  G    GMNAAV++ VR+G+  G  +  V +G +GL  G   I    W+ V G    GG+V+G+ R L  K      + + N+    I  LVIIGG  + +G     Q    Y +  I   VI +                ++ N+  G+D +IG ++AL  I    D +  + A + +R F+IE MG +CGYLA ++ +++ A   +I E K    + E ++   +E+ K++ +R          + + N+   A Y  D I +    + +        VLGH+Q+GG PT FDR  A + GA+++  L +  +          N +  ACV+ + K  ++ + L +   +T
Sbjct:  403 NVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQG--KIEPINWNSVGGWTGKGGSVLGTKRVLPAKHIE---EISLNIAKHNIHALVIIGGFEAFSGGLELVQARQKYEELCIPMVVIPA----------------TVSNNVPGSDFSIGADTALNTITSTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLAAGADAAYIYEDKFGIRDLEANVEHLVEKMKTTVKR---------GLILRNENCNANYTTDFIFNLYSEEGKGIFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKSVLWLTEKLKECYRHGRIFANSADSACVLGMKKRGLMFQALAELKEET 731

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Match: pfkpb (ATP-dependent 6-phosphofructokinase, platelet type-like [Source:NCBI gene;Acc:103025406])

HSP 1 Score: 477.633 bits (1228), Expect = 0.000e+0
Identity = 255/519 (49.13%), Postives = 345/519 (66.47%), Query Frame = 2

Query:   65 KFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            KF       G  I VLTSGGD+QGMNAAV++VVRMG+Y  A VYF+ EG QG++DGG NI+EA W  VS M+ LGGTVIGSARC +F+T  GRL+AA NL+   I NL +IGGDGSLTGAN+F+ EW+  ++EL++   ID         L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL E ++  +R+N++II+EGAID+HN+PIT  Y+KD+++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A+  P  PACV+ +  N+ +R PLM+CV  T  +Q  M    F + V +RG SF  N   Y+ L+   P         + +SK S              + ++  G  A GMNA +RS VR+ I        ++ GF+G +K  I  + W DV G    GGS++GT R LP+      +A ++    ++AL++IGGFE
Sbjct:    6 KFFENLSGAGKCIGVLTSGGDAQGMNAAVRAVVRMGIYVRAKVYFIHEGYQGMVDGGDNIKEATWESVSSMLQLGGTVIGSARCKEFRTHEGRLRAALNLVRLGITNLCVIGGDGSLTGANLFRTEWSGLLEELVQTGQIDEQAAVTHSALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPELPPEDGWEEQMCQKLSENRADKKRLNIIIIAEGAIDVHNKPITTDYVKDLVVRSLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEAS--PDTPACVVSLRGNQAIRLPLMECVQMTQQVQKAMDEKNFEEAVKLRGRSFENNLNTYKLLSYRKPD--------SEVSKSS------------FNVAVLNVGAPAAGMNAAVRSAVRVGITEGHTMFAVQDGFEGFSKGQINEIKWGDVGGWTGQGGSLLGTKRTLPAN-HLNKIAEQMRIHNINALLVIGGFE 501

HSP 2 Score: 194.512 bits (493), Expect = 0.000e+0
Identity = 100/257 (38.91%), Postives = 156/257 (60.70%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY---------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            EL   + K+    +P+V++P+T++NNIPG   ++G+DT +N I NT D +K  + GT+ R++I ET+G  CGYL T+GALA GAD +YI EE   +  L  +V  L  K+   I+ GLVLRN   +  + + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +AI W+   L+ +           DS  +LG+ +       P+    E +D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  508 ELLAARSKHPELCVPMVMVPSTVSNNIPGSDLSIGADTALNAITNTCDRIKQSASGTKRRVFIIETMGGYCGYLATVGALAAGADAAYIYEEPFTIRDLQTNVEHLTEKMKTGIQRGLVLRNESCNENFTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKIAAKAIQWISKKLKEFYVEGRVFANTEDSACLLGMRRRALV-FQPVTELKEESDFVHRIPKEQWWLKLRPLMKILAKYKTSYDVS 763

HSP 3 Score: 116.316 bits (290), Expect = 2.747e-26
Identity = 99/349 (28.37%), Postives = 167/349 (47.85%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQ--YXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMD 1105
            ++AVL  G  + GMNAAV+S VR+G+  G T++ V +G +G   G +N  E  W DV G    GG+++G+ R L     +   K A+ +    I  L++IGG  +  G              L+E     S   + C  + +V +  ++ N+  G+D++IG ++AL  I    D +  +A  + +R F+IE MG +CGYLA + A+++ A   +I E+P          + L E   +  +  LV+ +E      N+  T    Y       +   D R  VLGH+Q+GG P+ FDR    +  A+AI  +    +          N    AC++ + +  +V +P+ +
Sbjct:  403 NVAVLNVGAPAAGMNAAVRSAVRVGITEGHTMFAVQDGFEGFSKGQIN--EIKWGDVGGWTGQGGSLLGTKRTLPANHLN---KIAEQMRIHNINALLVIGGFEAYLG--------------LLELLAARSKHPELC--VPMVMVPSTVSNNIPGSDLSIGADTALNAITNTCDRIKQSASGTKRRVFIIETMGGYCGYLATVGALAAGADAAYIYEEPFTIRDLQTNVEHLTEKMKTGIQRGLVLRNESC----NENFTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKIAAKAIQWISKKLKEFYVEGRVFANTEDSACLLGMRRRALVFQPVTE 726

HSP 4 Score: 68.1662 bits (165), Expect = 2.641e-11
Identity = 53/199 (26.63%), Postives = 92/199 (46.23%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIH 2258
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD   I E         Q    L     D+ +  +++      +V + P  + Y+  L  +     FD R   +GHV  GG  S+FDR L +R G+EA+              + +L  S DT A V+ + 
Sbjct:  158 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPELPPEDGWEEQMCQKLSENRADKKRLNIIIIAEGAIDVHNKPITTDYVKDLVVRSLG--FDTRVTILGHVQRGGTPSAFDRILASRMGVEAV--------------LALLEASPDTPACVVSLR 339

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BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Match: pfkmb (phosphofructokinase, muscle b [Source:ZFIN;Acc:ZDB-GENE-081114-1])

HSP 1 Score: 471.855 bits (1213), Expect = 0.000e+0
Identity = 253/510 (49.61%), Postives = 337/510 (66.08%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++ VR+G+Y GA VYFV EG QGL+DGG +IR A W  VS M+ LGGTVIGSARC DF+T+ GR KAA NL+   I NL +IGGDGSLTGAN F+ EW+  +  L++   I + + +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR F++EVMGRHCGYLAL++A++S A +VFIPE PP+D WED LC++L   +S   R+N++I++EGA+D   +PIT + IK+++  ++  DTR T+LGHVQRGG P++FDRIL  R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+   M  G F + V +RG SF  N+  Y+ L  + P   K                      ++  I +M  G    GMNA +RS VR+ I    + + I  GF+GL    I P+ W  V      GGS +GT R LP+ +  E ++  + +F + AL+IIGGFE
Sbjct:  119 GKAIAVLTSGGDAQGMNAAVRATVRVGIYTGAKVYFVHEGYQGLVDGGDHIRLATWESVSMMLQLGGTVIGSARCKDFRTKEGRTKAACNLVKLGITNLCVIGGDGSLTGANEFRTEWSELLQILLKAGKITAEEARRSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITTTAQSHQRAFILEVMGRHCGYLALVSALASGADWVFIPEMPPDDGWEDHLCRRLANQRSLGYRLNVIIVAEGAMDRSGKPITCEMIKNLVTKKLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEA--TPETPACVVSLSGNQAVRLPLMECVQVTKDVTKAMTEGRFEEAVKLRGKSFENNWNTYKLLAHVNPAEVK----------------------SNINIALMNVGAPCSGMNAAVRSAVRIGILQGHHMLAIHDGFEGLAHGNIEPISWAAVGNWTGQGGSNLGTKRSLPASM-MEEISLNIAKFNIHALVIIGGFE 603

HSP 2 Score: 204.527 bits (519), Expect = 0.000e+0
Identity = 108/261 (41.38%), Postives = 159/261 (60.92%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            + G EL   ++KY+   IP+V+IPAT++NN+PG  F+VG+DT +N I +T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GL+LRN   +  Y + +IF L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+ G +A++W+ D L E Y +        DS  +LG+ +       P+     N D+E   P  QWW+KLRP+L+I+  ++   + S
Sbjct:  606 VGGLELVTGREKYEELCIPIVVIPATVSNNVPGSDFSVGADTALNTITSTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIYEEPFGIHDLEINVEHLVEKMKTTVKRGLILRNENCNSNYTTDFIFNLYTEEGKGVFDCRKNVLGHMQQGGTPSPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRALV-FQPLADLKSNTDFEHRIPKTQWWLKLRPILKILAKYKISLDIS 865

HSP 3 Score: 113.235 bits (282), Expect = 3.175e-25
Identity = 100/353 (28.33%), Postives = 170/353 (48.16%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            +IA++  G    GMNAAV+S VR+G+  G  +  + +G +GL  G  NI    W+ V      GG+ +G+ R L         + + N+    I  LVIIGG  +  G           ++ +  R+  + +    C  + IV +  ++ N+  G+D ++G ++AL  I    D +  + A + +R F+IE MG +CGYLA +  +++ A   +I E+P    + E ++   +E+ K++ +R  L++ +E      N      +       +   D R  VLGH+Q+GG P+ FDR    + GA+A+  L D  +          N    ACV+ + K  +V +PL D  S T
Sbjct:  505 NIALMNVGAPCSGMNAAVRSAVRIGILQGHHMLAIHDGFEGLAHG--NIEPISWAAVGNWTGQGGSNLGTKRSLPASMME---EISLNIAKFNIHALVIIGGFEAFVGG----------LELVTGREKYEEL----C--IPIVVIPATVSNNVPGSDFSVGADTALNTITSTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIYEEPFGIHDLEINVEHLVEKMKTTVKR-GLILRNENCNS--NYTTDFIFNLYTEEGKGVFDCRKNVLGHMQQGGTPSPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRALVFQPLADLKSNT 833

HSP 4 Score: 72.4034 bits (176), Expect = 1.371e-12
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
            +V +  +I N+  G    +G+D+ ++ I+  +D +   +   Q R +I E +G +CGYL  + ALA GAD  +I E              + N  SL   +N++       +  G + R   S  P     I  L  +  K  FD R   +GHV  GG  S+FDR LG+R G+EA++    +LE        ++ LS + +  +
Sbjct:  262 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITTTAQSHQ-RAFILEVMGRHCGYLALVSALASGADWVFIPEMPPDDGWEDHLCRRLANQRSLGYRLNVII------VAEGAMDR---SGKPITCEMIKNLVTK--KLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVM---ALLEATPETPACVVSLSGNQAVRL 450

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Match: pfkla (ATP-dependent 6-phosphofructokinase, liver type-like [Source:NCBI gene;Acc:103032887])

HSP 1 Score: 464.537 bits (1194), Expect = 0.000e+0
Identity = 253/510 (49.61%), Postives = 338/510 (66.27%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++V RMG+Y GA VY + EG QGL+DGG NI+ A+W  V+ ++ LGGTVIGSARC  F TR GR+ AA NL+ R I NL + GGDGSLTGANIF+ EW+  + EL+ +  I     K   +LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL++A++S A ++FIPE PP D WED +C +L E++S   R+N++I++EGAI+    PI++ YIKD+++ R+  DTR+TVLGHVQRGG P++FDR+L+ + G EA+ AL++A   P  PACVI +  N+ VR PLM+ V  T ++Q  M    F + + +RG SF  N+ IY+ L    P   K        S +S              + I+  G  A GMNA +RS VRM +A       +  GF+GL    +  + W+ V G    GGS++GT R LPS    E +   L ++ + +L+++GGFE
Sbjct:   15 GKAIAVLTSGGDAQGMNAAVRAVTRMGIYVGAKVYLIYEGYQGLVDGGDNIKLAHWHSVTNIIQLGGTVIGSARCKAFTTREGRVAAAFNLVKRGITNLCVCGGDGSLTGANIFRSEWSGLLAELVTKGRITPETAKKHDHLNIVGLVGSIDNDFCGTDMTIGADSALHRIMEIVDAITTTAISHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWEDVMCARLGESRSVGSRLNIIIVAEGAINKKGDPISSSYIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRVLSSKLGMEAVVALMEA--TPDTPACVIGLSGNQTVRLPLMEAVEMTKEVQKAMNEKRFEEAIQLRGRSFENNWNIYKLLAYQKPAQEK--------SNFS--------------VAILNVGAPAAGMNAAVRSAVRMGLAAGHRVYTVSDGFEGLANGEVNEVSWHSVAGWTGQGGSLLGTKRTLPSTC-MEKLVENLNKYNIQSLLVVGGFE 499

HSP 2 Score: 196.823 bits (499), Expect = 0.000e+0
Identity = 99/258 (38.37%), Postives = 155/258 (60.08%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKD-MLENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSF 2375
            EL+  +  YD   IP+ IIPATI+NN+PG  F++G+DT VN  + + D +K  + GT+ R+++ ET+G  CGYL T   +A GAD +YI E+  ++  L  +V  L  K+  +I+ GLVLRN  SH  Y + +I+ L+  EGKG+FDCR N +GH+  GG  S FDRN GT+ G+ A+ W+ + M + Y          D++ ++GL++  +    P+    +  D+E   P +QWW+     LR++    ++Y+TSF
Sbjct:  506 ELFDARGHYDELCIPMCIIPATISNNVPGTDFSLGADTAVNAAMESCDKIKQSATGTKRRVFVVETMGGYCGYLATTTGIAVGADAAYIYEDAFDIHELKTNVEHLTEKMKKDIQRGLVLRNEKSHKHYTTDFIYNLYSAEGKGVFDCRVNVLGHLQQGGAPSPFDRNFGTKLGVRAVQWLTEKMTDTYRQGRVFANSPDTVCVVGLNKK-ALTYCPVTEVKKQTDFEHRMPKEQWWLG----LRLLLKMLAKYQTSF 758

HSP 3 Score: 129.028 bits (323), Expect = 2.638e-30
Identity = 109/369 (29.54%), Postives = 173/369 (46.88%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTG-ANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDA-----------AQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLM 1141
            PA      S+A+L  G  + GMNAAV+S VRMGL  G  VY V +G +GL +G +N  E  W  V+G    GG+++G+ R L         K  +NL    I +L+++GG  +  G   +F      + DEL             C  + I+    +I N+  GTD ++G ++A+   ME+ D +  +A  + +R FV+E MG +CGYLA  T I+  A   +I E   + +      + L E    + +  LV+ +E +   +       Y       +   D R+ VLGH+Q+GG P+ FDR    + G  A+  L +            A +P    CV+ + K  +   P+ + V K  D ++ M
Sbjct:  393 PAQEKSNFSVAILNVGAPAAGMNAAVRSAVRMGLAAGHRVYTVSDGFEGLANGEVN--EVSWHSVAGWTGQGGSLLGTKRTLPSTCME---KLVENLNKYNIQSLLVVGGFEAYEGILELFDAR--GHYDEL-------------CIPMCII--PATISNNVPGTDFSLGADTAVNAAMESCDKIKQSATGTKRRVFVVETMGGYCGYLATTTGIAVGADAAYIYEDAFDIHELKTNVEHLTEKMKKDIQRGLVLRNEKSHKHYTTDFI--YNLYSAEGKGVFDCRVNVLGHLQQGGAPSPFDRNFGTKLGVRAVQWLTEKMTDTYRQGRVFANSPDT-VCVVGLNKKALTYCPVTE-VKKQTDFEHRM 735

HSP 4 Score: 73.1738 bits (178), Expect = 8.006e-13
Identity = 61/208 (29.33%), Postives = 102/208 (49.04%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN--------NVSHHPYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFE 2270
            +V +  +I N+  G    +G+D+ ++ I+  +D +   +I  Q R ++ E +G +CGYL  + ALA GAD  +I E     E   +DV  + A+L +    G  L          N    P  S YI  L  K+ G   +D R   +GHV  GG  S+FDR L ++ G+EA++    ++E   +    ++GLS + +   +P+    E
Sbjct:  158 IVGLVGSIDNDFCGTDMTIGADSALHRIMEIVDAITTTAISHQ-RTFVLEVMGRHCGYLALVSALASGADWLFIPEA--PPEDGWEDV--MCARLGESRSVGSRLNIIIVAEGAINKKGDPISSSYIKDLVVKRLG---YDTRVTVLGHVQRGGTPSAFDRVLSSKLGMEAVV---ALMEATPDTPACVIGLSGNQTVR-LPLMEAVE 353

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007452.1 (pep scaffold:Pmarinus_7.0:GL477249:14695:58282:1 gene:ENSPMAG00000006711.1 transcript:ENSPMAT00000007452.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 463.381 bits (1191), Expect = 8.316e-175
Identity = 257/525 (48.95%), Postives = 345/525 (65.71%), Query Frame = 2

Query:   47 LKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +   + KF+ +    G +I VLTSGGD+QGMNAAV++VVRMG+Y GA VYF+ EG QG++DGG +I+EA W  VS ++ LGGTVIGSARC DF+ R GRLKAA NL+ R I NL +IGGDGSLTGAN+F++EW+  ++EL+    I     +   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR FV+EVMGRHCGYLAL+TA+   A + FIPE PP D WED +C  L + +    R+N+VI++EGAID H +PIT+  I+ +I   +  DTR+T+LGHVQRGG P++FDRIL  R G EA+ AL++AA +   PACV+ +  N+ VR PLM+CV  T D+Q  M    F + V +RG SF  N  IY  L      H+K           SE K+      +   + ++C G  A GMNA +R+ +R+ I+       +  GF+GL K  +    W DV G    GG+ +GT R LP++   + +  ++ +  +SAL+IIGGFE
Sbjct:    1 MAPSDKKFLESLSGAGRAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGEHIQEASWDRVSSILQLGGTVIGSARCKDFREREGRLKAALNLVQRGISNLCVIGGDGSLTGANLFREEWSGLLEELVNNDQITGEMAQKFTHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITTTAQSHQRSFVLEVMGRHCGYLALVTALVCGADWTFIPECPPEDGWEDKMCSTLSDTRGRGVRLNIVIVAEGAIDRHGKPITSDSIRQLIKKCLGFDTRVTILGHVQRGGTPSAFDRILGSRMGVEAVLALLEAAAD--TPACVVSLSGNQAVRLPLMECVEMTKDVQKAMDECRFDEAVKLRGRSFENNLNIYRLL-----AHKK-----------SEIKK------SGFSVAVLCVGAPAAGMNAAVRAAIRVGISEGHRMYAVHDGFEGLAKGQVEEFGWGDVGGWTGRGGATLGTKRTLPNKF-LDKIVEQIQKHSISALLIIGGFE 500

HSP 2 Score: 169.474 bits (428), Expect = 8.316e-175
Identity = 85/211 (40.28%), Postives = 122/211 (57.82%), Query Frame = 3

Query: 1611 EALSGQ-ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLR------------------NNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLE 2186
            EAL G  +L   + K++   IP+ +IPATI+NN+PG  F++G+DT +N IV T D +K  + GT++R++I ET+G  CGYL TM  LA GAD +YI EE  ++  L  +V  L  K+   I  GLV+R                  N   +  Y + +I+ L+  EGKG+FDCR N +GH+  GG  S FDRN GT+   + + WV   L+
Sbjct:  500 EALEGAMQLASGRSKFEELCIPMCVIPATISNNVPGTDFSIGTDTALNAIVETCDRIKQSASGTKHRVFIIETMGGYCGYLATMSGLAAGADAAYIYEEPFSIRDLQMNVEHLTEKMKGNIHRGLVIRIRLPFTSLVIKLLPPFLINENCNPNYTTDFIYHLYSAEGKGVFDCRKNVLGHMQQGGCPSPFDRNFGTKIAAKGVQWVSKKLQ 710

HSP 3 Score: 114.39 bits (285), Expect = 1.756e-26
Identity = 96/325 (29.54%), Postives = 147/325 (45.23%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVI-------------ISEGAIDMHNQPITAQ---YXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIH 1015
            G S+AVL  G  + GMNAAV++ +R+G+  G  +Y V +G +GL  G   + E  W DV G    GG  +G+ R L  K      K  + +    I  L+IIGG  +L GA       + + +  I   VI +                +I N+  GTD +IG ++AL  I+E  D +  +A  +  R F+IE MG +CGYLA ++ +++ A   +I E+P +        + L E    N    LVI             +    I+ +  P       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+ + 
Sbjct:  400 GFSVAVLCVGAPAAGMNAAVRAAIRVGISEGHRMYAVHDGFEGLAKG--QVEEFGWGDVGGWTGRGGATLGTKRTLPNKFLD---KIVEQIQKHSISALLIIGGFEALEGAMQLASGRSKFEELCIPMCVIPA----------------TISNNVPGTDFSIGTDTALNAIVETCDRIKQSASGTKHRVFIIETMGGYCGYLATMSGLAAGADAAYIYEEPFSIRDLQMNVEHLTEKMKGNIHRGLVIRIRLPFTSLVIKLLPPFLINENCNPNYTTDFIYHLYSAEGKGVFDCRKNVLGHMQQGGCPSPFDRNFGTKIAAKGVQ 703

HSP 4 Score: 61.2326 bits (147), Expect = 6.673e-10
Identity = 46/163 (28.22%), Postives = 80/163 (49.08%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVL--RNNVSHH--PYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCG 2150
            +V +  +I N+  G    +G+D+ ++ I+  +D +   +  +  R ++ E +G +CGYL  + AL  GAD ++I E        ++  + L       ++  +V+     +  H  P  S  I +L K+     FD R   +GHV  GG  S+FDR LG+R G
Sbjct:  159 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITT-TAQSHQRSFVLEVMGRHCGYLALVTALVCGADWTFIPECPPEDGWEDKMCSTLSDTRGRGVRLNIVIVAEGAIDRHGKPITSDSIRQLIKKCLG--FDTRVTILGHVQRGGTPSAFDRILGSRMG 318

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008968.1 (pep scaffold:Pmarinus_7.0:GL478229:3907:42159:-1 gene:ENSPMAG00000008107.1 transcript:ENSPMAT00000008968.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 459.529 bits (1181), Expect = 3.592e-154
Identity = 249/497 (50.10%), Postives = 338/497 (68.01%), Query Frame = 2

Query:  134 GMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIER-KVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            GMNAAV++VVRMG+Y GA VYFV EG QG++DGG +I EA W  VS ++ LGGTVIGSARC +F+TR GRL AA +L+ R I NL +IGGDGSLTGANIF++EW+  + ELI++ K+ + + ++Y  +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WE+ +C KL E +   +R+N++I+SEGAID  N+PI++Q IKD++   + +DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N+ +R PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L        K V      S+++              I ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP +   + +A ++ E  ++AL+IIGGFE
Sbjct:    1 GMNAAVRAVVRMGIYVGAKVYFVHEGYQGMVDGGDHIEEANWESVSSILQLGGTVIGSARCKEFRTREGRLAAAFHLVQRGISNLCVIGGDGSLTGANIFREEWSGLLQELIKQGKISNDVAERY-SHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPESPPEEGWEEKMCTKLTENRLRKKRLNVMIVSEGAIDSFNKPISSQKIKDLVTKSLGLDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEA--TPETPACVVSLSGNQSIRLPLMECVQLTQEVQKAMDERRFADAVKLRGRSFENNLNTYKLL------ANKKVDSAYPKSQFT--------------IAVLNVGAPAAGMNAAVRSAVRVGITEGHNILAVNDGFEGFAKGQIKEIKWRDVGGWTGQGGSLLGTKRTLPKKY-LDQIAEKIRENNVNALLIIGGFE 473

HSP 2 Score: 72.0182 bits (175), Expect = 2.105e-13
Identity = 63/211 (29.86%), Postives = 102/211 (48.34%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGG-DGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTA 637
            + F    +  + L   K     P   F   +IAVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I+E  W DV G    GG+++G+ R L  K      + A+ + +  +  L+IIGG +  L+G             EL+E +  DS    +C  + +V +  ++ N+  G+D ++G ++AL  I   TD  +T A
Sbjct:  348 RSFENNLNTYKLLANKKVDSAYPKSQF---TIAVLNVGAPAAGMNAAVRSAVRVGITEGHNILAVNDGFEGFAKG--QIKEIKWRDVGGWTGQGGSLLGTKRTLPKKYLD---QIAEKIRENNVNALLIIGGFEAFLSGL------------ELVEAR--DS-HPAFC--IPMVVVPATVSNNVPGSDFSVGADTALNTI---TDVSVTHA 530

HSP 3 Score: 64.3142 bits (155), Expect = 6.955e-11
Identity = 55/197 (27.92%), Postives = 92/197 (46.70%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------EVLNLESLNQDVNILKAKLFDE--IKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTS 2237
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E       E      L +  N L+ K  +   +  G +   N    P  S  I  L  +      D R   +GHV  GG  S+FDR L +R G+EA++    +LE        ++ LS + S
Sbjct:  130 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPESPPEEGWEEKMCTKLTE--NRLRKKRLNVMIVSEGAIDSFN---KPISSQKIKDLVTKSLG--LDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLEATPETPACVVSLSGNQS 315

HSP 4 Score: 58.5362 bits (140), Expect = 3.580e-9
Identity = 24/49 (48.98%), Postives = 37/49 (75.51%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVN 1763
            LSG EL + +D +  F IP+V++PAT++NN+PG  F+VG+DT +N I +
Sbjct:  476 LSGLELVEARDSHPAFCIPMVVVPATVSNNVPGSDFSVGADTALNTITD 524

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005911.1 (pep scaffold:Pmarinus_7.0:GL478489:7235:45113:1 gene:ENSPMAG00000005246.1 transcript:ENSPMAT00000005911.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 370.933 bits (951), Expect = 3.290e-144
Identity = 212/481 (44.07%), Postives = 287/481 (59.67%), Query Frame = 2

Query:  185 ATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNR--RVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            A V    +G QG++DGG NIREA W  VSG++ LGGTVIGSARC DF+ R GR +AA +L++  I NL IIGGDGSLTGAN+F+QEW  ++  L+++  I       C NL++VGLVGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP D WED +C KL   ++  R  R+N   ++ G ++     +   Y K   I       ++  LG +Q+   P SFDR  A R G EA+ AL++A   P  PA V+ +  N+IVR  LM CV +T  +Q  M  G F D V +RG SF  N   Y                    + ++  K  +  + T   + ++  G  A G+NA +RS VR+ +   L  + +  GF GL    +  + W+ V G    GGSI+GT R LP +I  EVV S + +  + AL++IGGFE
Sbjct:    1 ARVRLCFQGYQGMVDGGDNIREATWESVSGILQLGGTVIGSARCKDFRGRDGREQAALHLLEHGITNLCIIGGDGSLTGANMFRQEWGEHVQSLLKQGKISEEVAGRCSNLHVVGLVGSIDNDFCGTDMTIGADSALHRIVEVVDAIMTTATSHQRTFVLEVMGRHCGYLALVSALTCGADWVFIPESPPQDGWEDRMCDKLSAVRAVPRRKRINENTLAHGHVEHLIAGLQTVY-KGGAIRIAAESKKLRTLGELQK--TPPSFDREEASRMGFEAVLALLEA--TPETPAYVVALTGNQIVRNSLMQCVQQTQAVQKAMDEGRFEDAVRLRGKSFQNNLNSY--------------------NLFANNKAPVSKAKTAFTVAVLSVGAPAAGVNAAVRSAVRVGMTCGLRVLTVNDGFQGLASGQVQEMTWDSVGGWTGQGGSILGTKRTLPGKILKEVV-SEIQKNDIQALLVIGGFE 455

HSP 2 Score: 160.229 bits (404), Expect = 3.290e-144
Identity = 83/189 (43.92%), Postives = 127/189 (67.20%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENY 2192
            EL   + ++D   IP+V++PAT++NN+PG  F +G+DT +N+I++T D++K  + GT+ R+++ ET+G  CGYL T GALA GAD +YI EE   +  L  DV  L  K+   ++ GLVLRN   +  Y + +++ L+ +EGKG+FDCR N +GH+  GG+ S FDRN GT+   +AI W+ + L E+Y
Sbjct:  462 ELEAARAQHDELCIPMVVVPATVSNNVPGTDFCLGADTALNIIMHTCDHIKQSASGTKRRVFVIETMGGFCGYLATAGALAAGADAAYIYEEPFGIAQLQADVIRLVEKMKSGVQRGLVLRNEHCNTNYSTDFVYHLYTEEGKGVFDCRKNVLGHMQQGGVPSPFDRNFGTKMAAKAIQWLSEKLKESY 650

HSP 3 Score: 113.235 bits (282), Expect = 3.805e-26
Identity = 98/311 (31.51%), Postives = 159/311 (51.13%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQ-IFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHAL 1021
            ++AVL+ G  + G+NAAV+S VR+G+ CG  V  V +G QGL  G   ++E  W  V G    GG+++G+ R L  K     LK   + I +  I  L++IGG             + +Y+  ++E +   +   + C  + +V +  ++ N+  GTD  +G ++AL  IM   D +  +A  + +R FVIE MG  CGYLA   A+++ A   +I E+P      + D+ + +E+ KS  +R  LV+ +E     ++      Y       +   D R  VLGH+Q+GG P+ FDR    +  A+AI  L
Sbjct:  357 TVAVLSVGAPAAGVNAAVRSAVRVGMTCGLRVLTVNDGFQGLASG--QVQEMTWDSVGGWTGQGGSILGTKRTLPGKI----LKEVVSEIQKNDIQALLVIGG-------------FEAYLG-VLELEAARAQHDELC--IPMVVVPATVSNNVPGTDFCLGADTALNIIMHTCDHIKQSASGTKRRVFVIETMGGFCGYLATAGALAAGADAAYIYEEPFGIAQLQADVIRLVEKMKSGVQR-GLVLRNEHCNTNYSTDFV--YHLYTEEGKGVFDCRKNVLGHMQQGGVPSPFDRNFGTKMAAKAIQWL 642

HSP 4 Score: 50.8322 bits (120), Expect = 1.150e-6
Identity = 46/174 (26.44%), Postives = 75/174 (43.10%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTS---SFDRNLGTRCGLEAII 2165
            VV +  +I N+  G    +G+D+ ++ IV  +D + + +  +  R ++ E +G +CGYL  + AL  GAD  +I E       E    + L+    + + K  +E         N   H +  H I  L      G       S    +LG L     SFDR   +R G EA++
Sbjct:  113 VVGLVGSIDNDFCGTDMTIGADSALHRIVEVVDAI-MTTATSHQRTFVLEVMGRHCGYLALVSALTCGADWVFIPESPPQDGWEDRMCDKLSAVRAVPRRKRINE---------NTLAHGHVEHLIAGLQTVYKGGAIRIAAESKKLRTLGELQKTPPSFDREEASRMGFEAVL 276

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008974.1 (pep scaffold:Pmarinus_7.0:GL478229:3949:42166:-1 gene:ENSPMAG00000008107.1 transcript:ENSPMAT00000008974.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 390.578 bits (1002), Expect = 3.344e-127
Identity = 217/446 (48.65%), Postives = 298/446 (66.82%), Query Frame = 2

Query:  287 GGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIER-KVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            GGTVIGSARC +F+TR GRL AA +L+ R I NL +IGGDGSLTGANIF++EW+  + ELI++ K+ + + ++Y  +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +VFIPE PP + WE+ +C KL E +   +R+N++I+SEGAID  N+PI++Q IKD++   + +DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N+ +R PLM+CV  T ++Q  M    F D V +RG SF  N   Y+ L        K V      S+++              I ++  G  A GMNA +RS VR+ I      + +  GF+G  K  I  + W DV G    GGS++GT R LP +   + +A ++ E  ++AL+IIGGFE
Sbjct:   83 GGTVIGSARCKEFRTREGRLAAAFHLVQRGISNLCVIGGDGSLTGANIFREEWSGLLQELIKQGKISNDVAERY-SHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPESPPEEGWEEKMCTKLTENRLRKKRLNVMIVSEGAIDSFNKPISSQKIKDLVTKSLGLDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEA--TPETPACVVSLSGNQSIRLPLMECVQLTQEVQKAMDERRFADAVKLRGRSFENNLNTYKLLA------NKKVDSAYPKSQFT--------------IAVLNVGAPAAGMNAAVRSAVRVGITEGHNILAVNDGFEGFAKGQIKEIKWRDVGGWTGQGGSLLGTKRTLPKKY-LDQIAEKIRENNVNALLIIGGFE 504

HSP 2 Score: 70.4774 bits (171), Expect = 6.619e-13
Identity = 59/202 (29.21%), Postives = 98/202 (48.51%), Query Frame = 2

Query:    8 KEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGG-DGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIME 610
            + F    +  + L   K     P   F   +IAVL  G  + GMNAAV+S VR+G+  G  +  V +G +G   G   I+E  W DV G    GG+++G+ R L  K      + A+ + +  +  L+IIGG +  L+G             EL+E +  DS    +C  + +V +  ++ N+  G+D ++G ++AL  I +
Sbjct:  379 RSFENNLNTYKLLANKKVDSAYPKSQF---TIAVLNVGAPAAGMNAAVRSAVRVGITEGHNILAVNDGFEGFAKG--QIKEIKWRDVGGWTGQGGSLLGTKRTLPKKYLD---QIAEKIRENNVNALLIIGGFEAFLSGL------------ELVEAR--DS-HPAFC--IPMVVVPATVSNNVPGSDFSVGADTALNTITD 555

HSP 3 Score: 64.6994 bits (156), Expect = 5.147e-11
Identity = 49/192 (25.52%), Postives = 90/192 (46.88%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVS----HHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTS 2237
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E         +    L      + +  +++ +  +    + P  S  I  L  +      D R   +GHV  GG  S+FDR L +R G+EA++    +LE        ++ LS + S
Sbjct:  161 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPESPPEEGWEEKMCTKLTENRLRKKRLNVMIVSEGAIDSFNKPISSQKIKDLVTKSLG--LDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLEATPETPACVVSLSGNQS 346

HSP 4 Score: 58.9214 bits (141), Expect = 3.302e-9
Identity = 24/49 (48.98%), Postives = 37/49 (75.51%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVN 1763
            LSG EL + +D +  F IP+V++PAT++NN+PG  F+VG+DT +N I +
Sbjct:  507 LSGLELVEARDSHPAFCIPMVVVPATVSNNVPGSDFSVGADTALNTITD 555

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007362.1 (pep scaffold:Pmarinus_7.0:GL493177:1250:4583:-1 gene:ENSPMAG00000006644.1 transcript:ENSPMAT00000007362.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 250.366 bits (638), Expect = 2.384e-78
Identity = 128/212 (60.38%), Postives = 160/212 (75.47%), Query Frame = 2

Query:  476 KVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCV 1111
            K+   + KK   +LNIVG+VGSIDNDFCGTDMTIG +SAL RIME  DA+ TTA SHQR FV+EVMGRHCGYLAL+T ++  A FVF+PE PP D WED LC  L E +S   R+N++I++EGAID H + IT+  IKD++  R+  DTR+TVLGHVQRGG P++FDRILA R G EA+ AL+DA  +P  PACV+ +  N+ VR PLM+CV
Sbjct:    2 KITAEVAKKN-SHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRSFVLEVMGRHCGYLALVTGLTCGADFVFLPEMPPADGWEDQLCATLTETRSRGVRLNIIIVAEGAIDRHGKAITSDQIKDMVTKRLGFDTRVTVLGHVQRGGTPSAFDRILASRMGVEAVLALLDA--SPETPACVVSLSGNQAVRLPLMECV 210

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Yeast
Match: PFK2 (Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes [Source:SGD;Acc:S000004818])

HSP 1 Score: 345.125 bits (884), Expect = 4.998e-128
Identity = 203/511 (39.73%), Postives = 314/511 (61.45%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLN-IREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDN-WEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNK-KTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +IAV+TSGGD+ GMN+ V+++VR  ++ G   + V+EG +GL+ GG   I+E +W DV G    GGT IG+ARC++FK R GRL  A++LI+  +  L++ GGDGSLTGA++F+ EW S I+EL++   I + + +  K+LNI G VGSIDND   TD TIG  SAL RI +A D +  TA+SH R FV+EVMGR+CG+LAL+  I++ A ++FIPEKP   + W+D +C  + + +S  +R  +V+++EGAI     PI+   +  +++DR+ +DTRIT LGHVQRGG   ++DRILA  +G EA++A++++   P  P+ +I + +N+IVRKPLM+ V  T  +   ++   F   + +R   F  +   + A+N                + ++E K   D  +   +I I+  G  A G+N+ + S+    ++    P  I  G+ GL + +++  L W D+ G  S GGS IGT RV P +    ++A    +++   LII+GGFE
Sbjct:  198 AIAVMTSGGDAPGMNSNVRAIVRSAIFKGCRAFVVMEGYEGLVRGGPEYIKEFHWEDVRGWSAEGGTNIGTARCMEFKKREGRLLGAQHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRISNEQYERMKHLNICGTVGSIDNDMSTTDATIGAYSALDRICKAIDYVEATANSHSRAFVVEVMGRNCGWLALLAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAEGAIAADLTPISPSDVHKVLVDRLGLDTRITTLGHVQRGGTAVAYDRILATLQGLEAVNAVLES--TPDTPSPLIAVNENKIVRKPLMESVKLTKAVAEAIQAKDFKRAMSLRDTEFIEHLNNFMAINS---------------ADHNEPKLPKDKRL---KIAIVNVGAPAGGINSAVYSMATYCMSQGHRPYAIYNGWSGLARHESVRSLNWKDMLGWQSRGGSEIGTNRVTPEEADLGMIAYYFQKYEFDGLIIVGGFE 688

HSP 2 Score: 131.339 bits (329), Expect = 4.998e-128
Identity = 66/189 (34.92%), Postives = 114/189 (60.32%), Query Frame = 3

Query: 1626 QELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEI----KYG-LVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKD 2177
             +L + ++ Y  FRIP+V+IPAT++NN+PG  +++GSDT +N ++   D +K  +  T+ R ++ +  G N GYL T  +LA GA +SY+ EE ++LE L++D+  L A+ F++     ++G L+L++  +     +  + ++   E  G FD +    GHV  GGL S  DR   TR  ++A+ ++KD
Sbjct:  694 HQLERARESYPAFRIPMVLIPATLSNNVPGTEYSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQVSYVPEEGISLEQLSEDIEYL-AQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVITAEADGRFDAKPAYPGHVQQGGLPSPIDRTRATRMAIKAVGFIKD 881

HSP 3 Score: 80.1073 bits (196), Expect = 7.809e-16
Identity = 81/317 (25.55%), Postives = 141/317 (44.48%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYC---GATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQ-RCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALID 1027
            IA++  G  + G+N+AV S   M  YC   G   Y +  G  GL     ++R   W D+ G  + GG+ IG+ R    +   G +  A      +   L+I+GG  +    +  ++   SY                    + +V +  ++ N+  GT+ ++G ++AL  +ME  D +  +A S + R FV++  G + GYLA   +++  AQ  ++PE+  + +   +D   L +  E+A+   R   L++ S  A    +    A+ I      R          GHVQ+GG P+  DR  A R   +A+  + D
Sbjct:  589 IAIVNVGAPAGGINSAVYS---MATYCMSQGHRPYAIYNGWSGLARHE-SVRSLNWKDMLGWQSRGGSEIGTNRVTPEEADLGMI--AYYFQKYEFDGLIIVGGFEAFESLHQLERARESYP----------------AFRIPMVLIPATLSNNVPGTEYSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQVSYVPEEGISLEQLSEDIEYLAQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVITAEADGRFDAKPAYP--GHVQQGGLPSPIDRTRATRMAIKAVGFIKD 881

HSP 4 Score: 53.9138 bits (128), Expect = 8.725e-8
Identity = 42/165 (25.45%), Postives = 77/165 (46.67%), Query Frame = 3

Query: 1692 TIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEE-VLNLESLNQDVNIL-------KAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAI 2162
            +I N++      +G+ + ++ I   +D ++  +  +  R ++ E +G NCG+L  +  +A  AD  +I E+   + E  +Q  +I+       K      +  G +  +     P D H +  L  + G    D R  ++GHV  GG   ++DR L T  GLEA+
Sbjct:  346 SIDNDMSTTDATIGAYSALDRICKAIDYVE-ATANSHSRAFVVEVMGRNCGWLALLAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAEGAIAADLTPISPSDVHKV--LVDRLG---LDTRITTLGHVQRGGTAVAYDRILATLQGLEAV 504

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Yeast
Match: PFK1 (Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes [Source:SGD;Acc:S000003472])

HSP 1 Score: 335.495 bits (859), Expect = 1.277e-119
Identity = 209/518 (40.35%), Postives = 307/518 (59.27%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKP-PNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGL-NKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFEWTRIVEK*R 1648
            IAV+TSGGDS GMNAAV++VVR G++ G  V+ V EG +GL+ GG  +++  W DV G ++ GGT+IG+AR ++F+ R GR +AA NLI + I  LV+ GGDGSLTGA++F+ EW S +DEL+        +    KNL+IVGLVGSIDND  GTD TIG  SAL RI E  D +  TA SH R FV+EVMGRHCG+LAL+  I++ A ++FIPE+  P+  W+D+L +  +  +S  RR N +I++EGA+D    P+TA       +  + +DT++T+LGHVQRGG   + DR LA  +G +A+ A+++    P  P+ +I I++N+I+R PL++ V  T  +   + N  F   + +R   F   Y+ + +  V          +G+ +   S+R            IGI+  G  +  +NA  R+     +++   P  I  GF GL     +  L W DV    ++GGS IGT R + S+     +A    + KL  LII+GGFE  R +++ R
Sbjct:  208 IAVMTSGGDSPGMNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGTARSMEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDELVAEGRFTKEEVAPYKNLSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYIDATAKSHSRAFVVEVMGRHCGWLALMAGIATGADYIFIPERAVPHGKWQDELKEVCQRHRSKGRRNNTIIVAEGALDDQLNPVTAN-DVKDALIELGLDTKVTILGHVQRGGTAVAHDRWLATLQGVDAVKAVLEF--TPETPSPLIGILENKIIRMPLVESVKLTKSVATAIENKDFDKAISLRDTEFIELYENFLSTTV--------KDDGSELLPVSDR----------LNIGIVHVGAPSAALNAATRAATLYCLSHGHKPYAIMNGFSGLIQTGEVKELSWIDVENWHNLGGSEIGTNRSVASE-DLGTIAYYFQKNKLDGLIILGGFEGFRSLKQLR 703

HSP 2 Score: 113.235 bits (282), Expect = 1.277e-119
Identity = 67/244 (27.46%), Postives = 121/244 (49.59%), Query Frame = 3

Query: 1626 QELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLF---DEIKYG-LVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKD---MLENYDNDSIVILGLSQDTSANVIPIHTQFE--------NADYEKYRPLDQWW 2312
            ++L   + ++ IF IP+ +IPAT++NN+PG  +++G DT +N +VN  D++K  +  T+ R+++ E  G + GY+ +   L  GA   Y  E+ ++L S+ +D+ +LK        E + G L++RN  +   Y +  +  +  +  KG F  R    GHV  GG+ SS DR   +R  ++ I +++      E   N    +L    DT    +P     +        N  +  ++P+   W
Sbjct:  700 KQLRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTCLNALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLASIREDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLADIISEASKGKFGVRTAIPGHVQQGGVPSSKDRVTASRFAVKCIKFIEQWNKKNEASPNTDAKVLRFKFDTHGEKVPTVEHEDDSAAVICVNGSHVSFKPIANLW 943

HSP 3 Score: 87.8113 bits (216), Expect = 3.533e-18
Identity = 83/285 (29.12%), Postives = 134/285 (47.02%), Query Frame = 2

Query:  173 LYC---GATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCL---DFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDD---LCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALR 994
            LYC   G   Y ++ G  GLI  G  ++E  W DV    NLGG+ IG+ R +   D  T +   +  KN +D     L+I+GG     G    KQ             + D   +    N+ +  +  ++ N+  GT+ ++GV++ L  ++  TD +  +A  + +R FV EV G H GY+A  T + + A  V+ PEK  +  +  +D   L +     K  NR   L++ +E A  +++  + A         + K   R  + GHVQ+GG P+S DR+ A R
Sbjct:  617 LYCLSHGHKPYAIMNGFSGLIQTG-EVKELSWIDVENWHNLGGSEIGTNRSVASEDLGTIAYYFQ--KNKLD----GLIILGG---FEGFRSLKQ-------------LRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTCLNALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLASIREDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLAD--IISEASKGKFGVRTAIPGHVQQGGVPSSKDRVTASR 876

HSP 4 Score: 45.0542 bits (105), Expect = 2.059e-12
Identity = 41/133 (30.83%), Postives = 67/133 (50.38%), Query Frame = 2

Query: 1289 KRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNK--KTITPLLWNDVRXXXXXXXXXXXTTRVLP--SQIGFEVVASRLLEFKLSALIIIGG-----------FEWTRIVEK 1642
            ++ L +S    +I +M SG  + GMNA +R+VVR  I        +  G++GL +  K +  + W DVRG +S GG++IGT R +    + G    A  L+   + AL++ GG            EW  +V++
Sbjct:  196 RQTLGSSQKKKKIAVMTSGGDSPGMNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGTARSMEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDE 328

HSP 5 Score: 44.669 bits (104), Expect = 2.059e-12
Identity = 51/232 (21.98%), Postives = 94/232 (40.52%), Query Frame = 3

Query: 1668 IPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIK------YGLVLRNNV----------SHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQ---------DTSANVIPIHTQFENADYEK 2288
            + +V +  +I N++ G    +G+ + +  I   +D +   +  +  R ++ E +G +CG+L  M  +A GAD  +I E             +   K  DE+K           RNN             +P  ++ +     + G    D +   +GHV  GG   + DR L T  G++A   VK +LE        ++G+ +         ++      + T  EN D++K
Sbjct:  346 LSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYID-ATAKSHSRAFVVEVMGRHCGWLALMAGIATGADYIFIPERA-----------VPHGKWQDELKEVCQRHRSKGRRNNTIIVAEGALDDQLNPVTANDVKDALIELG---LDTKVTILGHVQRGGTAVAHDRWLATLQGVDA---VKAVLEFTPETPSPLIGILENKIIRMPLVESVKLTKSVATAIENKDFDK 559

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Match: pfkpa (ATP-dependent 6-phosphofructokinase, platelet type [Source:NCBI gene;Acc:101155970])

HSP 1 Score: 480.715 bits (1236), Expect = 0.000e+0
Identity = 250/510 (49.02%), Postives = 351/510 (68.82%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVRMGLY GA VYF+ EG QG++DGG NI EA W  VS M+ +GGTVIGSARC +F++  GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  + EL+E+ +I++   +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL   +S   R+N++I++EG++D H +PIT+ ++KD+++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++   +   PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N + Y+ L      HRK   E                 V++  + ++  G  A GMNA +RS VR+ I+       +  GF+G  K  I  + W+DV G    GGS++GT R LP++   + +A ++ ++ ++AL++IGGFE
Sbjct:   18 GKAIAVLTSGGDAQGMNAAVRAVVRMGLYVGAKVYFIHEGYQGMVDGGDNIEEASWESVSSMLQVGGTVIGSARCKEFRSHEGRLQAAHNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLKELVEKGLIEADAVERYAALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSATRSRGTRLNIIIVAEGSLDRHGKPITSSFVKDLVVKHLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLETTAD--TPACVVSLCGNQSVRLPLMECVQMTQEVQKAMDQKRFEEAVKLRGRSFENNLRTYKLL-----AHRKPESE---------------LPVSNFNVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKGQIKEVKWSDVGGWTGQGGSLLGTKRTLPAKHA-DKIAEQMRKYNINALLVIGGFE 504

HSP 2 Score: 200.675 bits (509), Expect = 0.000e+0
Identity = 105/257 (40.86%), Postives = 162/257 (63.04%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            +L++ +  Y+ F IP+ ++PATI+NN+PG   ++G+DT +N IV T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K +LE Y          DS  +LG+   T     P+ T  ++ D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  511 QLYEVRTTYEEFCIPMCMLPATISNNVPGTALSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENSNDNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKMSAKAMQWLSKKLLETYRRGRVFANSEDSCCLLGMLGRTLV-FQPVVTLKDHTDFIHRIPKEQWWLKLRPLMKILAKYKTSYDVS 766

HSP 3 Score: 125.561 bits (314), Expect = 3.167e-29
Identity = 99/330 (30.00%), Postives = 169/330 (51.21%), Query Frame = 2

Query:   38 EFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHAL 1021
            + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  ++ V +G +G   G   I+E  WSDV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +L              + L++   + +  +++C  + +  L  +I N+  GT ++IG ++AL  I+E  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + D +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  L
Sbjct:  389 KLLAHRKPESELPVSNF---NVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKG--QIKEVKWSDVGGWTGQGGSLLGTKRTLPAKHAD---KIAEQMRKYNINALLVIGGFEAL--------------ESLLQLYEVRTTYEEFC--IPMCMLPATISNNVPGTALSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQANVEHLTEKMKTSIQR-GLVLRNENSNDNYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKMSAKAMQWL 691

HSP 4 Score: 69.707 bits (169), Expect = 7.833e-12
Identity = 54/197 (27.41%), Postives = 98/197 (49.75%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE---EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHH--PYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD   I E   E    E + Q ++  +++    +   +V   ++  H  P  S ++  L  K  G   FD R   +GHV  GG  S+FDR L +R G+EA++    +LE   +    ++ L  + S  +
Sbjct:  161 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSATRSR-GTRLNIIIVAEGSLDRHGKPITSSFVKDLVVKHLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLETTADTPACVVSLCGNQSVRL 349

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Match: pfkpa (ATP-dependent 6-phosphofructokinase, platelet type [Source:NCBI gene;Acc:101155970])

HSP 1 Score: 479.174 bits (1232), Expect = 0.000e+0
Identity = 251/510 (49.22%), Postives = 352/510 (69.02%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G +IAVLTSGGD+QGMNAAV++VVRMGLY GA VYF+ EG QG++DGG NI EA W  VS M+ +GGTVIGSARC +F++  GRL+AA NL+ R I NL +IGGDGSLTGAN+F++EW+  + EL+E+ +I++   +    L+IVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA++TTA SHQR FV+EVMGRHCGYLAL++A++  A +V IPE PP D WE+ +C+KL   ++  +R+N++I++EGAID +N+ IT +YIK++++  +  DTR+T+LGHVQRGG P++FDRILA R G EA+ AL++   +   PACV+ +  N+ VR PLM+CV  T ++Q  M    F + V +RG SF  N + Y+ L      HRK   E                 V++  + ++  G  A GMNA +RS VR+ I+       +  GF+G  K  I  + W+DV G    GGS++GT R LP++   + +A ++ ++ ++AL++IGGFE
Sbjct:   18 GKAIAVLTSGGDAQGMNAAVRAVVRMGLYVGAKVYFIHEGYQGMVDGGDNIEEASWESVSSMLQVGGTVIGSARCKEFRSHEGRLQAAHNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLKELVEKGLIEADAVERYAALHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSANRAGMKRLNIIIVAEGAIDRNNKAITTEYIKNLVVKHLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLETTAD--TPACVVSLCGNQSVRLPLMECVQMTQEVQKAMDQKRFEEAVKLRGRSFENNLRTYKLL-----AHRKPESE---------------LPVSNFNVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKGQIKEVKWSDVGGWTGQGGSLLGTKRTLPAKHA-DKIAEQMRKYNINALLVIGGFE 504

HSP 2 Score: 200.675 bits (509), Expect = 0.000e+0
Identity = 105/257 (40.86%), Postives = 162/257 (63.04%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWV-KDMLENY--------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            +L++ +  Y+ F IP+ ++PATI+NN+PG   ++G+DT +N IV T D +K  + GT+ R++I ET+G  CGYL T+G LA GAD +YI EE  ++  L  +V  L  K+   I+ GLVLRN  S+  Y + +I++L+ +EGKG+FDCR N +GH+  GG  S FDRN GT+   +A+ W+ K +LE Y          DS  +LG+   T     P+ T  ++ D+    P +QWW+KLRPL++I+  +++ Y+ S
Sbjct:  511 QLYEVRTTYEEFCIPMCMLPATISNNVPGTALSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENSNDNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKMSAKAMQWLSKKLLETYRRGRVFANSEDSCCLLGMLGRTLV-FQPVVTLKDHTDFIHRIPKEQWWLKLRPLMKILAKYKTSYDVS 766

HSP 3 Score: 125.561 bits (314), Expect = 3.085e-29
Identity = 99/330 (30.00%), Postives = 169/330 (51.21%), Query Frame = 2

Query:   38 EFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHAL 1021
            + L   K    +P   F   ++AVL  G  + GMNAAV+S VR+G+  G  ++ V +G +G   G   I+E  WSDV G    GG+++G+ R L  K      K A+ +    I  L++IGG  +L              + L++   + +  +++C  + +  L  +I N+  GT ++IG ++AL  I+E  D +  +A  + +R F+IE MG +CGYLA +  +++ A   +I E+P +  + + ++    E+ K+S +R  LV+ +E + D +       Y       +   D R  VLGH+Q+GG P+ FDR    +  A+A+  L
Sbjct:  389 KLLAHRKPESELPVSNF---NVAVLNVGAPAAGMNAAVRSAVRVGISEGHKMFAVNDGFEGFYKG--QIKEVKWSDVGGWTGQGGSLLGTKRTLPAKHAD---KIAEQMRKYNINALLVIGGFEAL--------------ESLLQLYEVRTTYEEFC--IPMCMLPATISNNVPGTALSIGADTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLATVGGLAAGADAAYIYEEPFDIRDLQANVEHLTEKMKTSIQR-GLVLRNENSNDNYTTDFI--YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKMSAKAMQWL 691

HSP 4 Score: 69.707 bits (169), Expect = 8.389e-12
Identity = 56/199 (28.14%), Postives = 95/199 (47.74%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD   I E       E    + L+ +   +K      +  G + RNN       + YI  L  K  G   FD R   +GHV  GG  S+FDR L +R G+EA++    +LE   +    ++ L  + S  +
Sbjct:  161 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAI-MTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVLIPEMPPEDGWEEKMCQKLSANRAGMKRLNIIIVAEGAIDRNN---KAITTEYIKNLVVKHLG---FDTRVTILGHVQRGGTPSAFDRILASRMGVEAVL---ALLETTADTPACVVSLCGNQSVRL 349

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Match: pfkma (ATP-dependent 6-phosphofructokinase, muscle type [Source:NCBI gene;Acc:101163079])

HSP 1 Score: 477.633 bits (1228), Expect = 0.000e+0
Identity = 259/510 (50.78%), Postives = 338/510 (66.27%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G SIAVLTSGGD+QGMNAAV++ VR+GLY GA VYFV EG QGL+DGG NI  A W  VS M+ LGGTVIGSARC DF+TR GR+KAA NL+   I NL +IGGDGSLTGAN F+ EW+  + +L+    I + + K   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR F++EVMGRHCGYLAL++A++  A +VFIPE PP++ WED LC++L + +S   R+N++I++EGAI    +PIT+  IK ++ DR+  DTR TVLGHVQRGG P++FDRIL  R G EA+ AL++A   P  PACV+ +  N+ VR PLM+CV  T D+   M  G F D + +RG SF  N+  Y+ L  + P   K                      ++  + +M  G    GMNA +RS V+M I      + +  GFDGL    I P+ W  V G   MGGS++GT R LP ++  E V+  + ++ + AL+IIGGFE
Sbjct:   17 GKSIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVYFVHEGYQGLVDGGENICSASWESVSMMLQLGGTVIGSARCKDFRTREGRMKAACNLVKLGITNLCVIGGDGSLTGANEFRTEWSGLLADLVRAGKISAEEAKKSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITTTAQSHQRTFILEVMGRHCGYLALVSALACGADWVFIPEMPPDEGWEDHLCRRLMDQRSRGSRLNVIIVAEGAISRDGKPITSDQIKKLVTDRLGFDTRTTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEA--TPDTPACVVSLSGNQAVRLPLMECVQVTKDVTAAMAQGKFEDAIKLRGRSFENNWNTYKLLAHINPPDVK----------------------SNINVAVMNIGAPCAGMNAAVRSAVKMGIIQGHSMLAVHDGFDGLAHGQIEPITWTSVSGWTGMGGSMLGTKRTLPGKM-MEEVSLNIAKYNIHALVIIGGFE 501

HSP 2 Score: 202.986 bits (515), Expect = 0.000e+0
Identity = 102/261 (39.08%), Postives = 163/261 (62.45%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDML-ENYDN--------DSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            + G EL + ++KY+   IP+V+IPAT++NN+PG  F++G+DT +N I +T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE   +  L  +V  L  K+   +K GL+LRN   +  Y + +IF L+ +EGKG+FDCR N +GH+  GG  + FDRN  T+ G ++++W+ + L E Y +        DS  +LG+ +  + N  P+     + D+E   P  QWW+K+RP+++I+  ++ + +TS
Sbjct:  504 VGGLELVQAREKYEEVCIPIVVIPATVSNNVPGSDFSIGADTALNTITSTCDRIKQSAAGTKRRVFIVETMGGYCGYLATMAGLAAGADAAYIFEEKFTIRDLEANVEHLLEKMKTTVKRGLILRNENCNSNYTTDFIFSLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKSVLWLTEKLKECYRHGRIFANTPDSACVLGM-RKRALNFQPLADLKADTDFEHRIPKTQWWLKIRPIMKILAKYDIKLDTS 763

HSP 3 Score: 119.783 bits (299), Expect = 2.292e-27
Identity = 103/353 (29.18%), Postives = 170/353 (48.16%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFI-PEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            ++AV+  G    GMNAAV+S V+MG+  G ++  V +G  GL  G   I    W+ VSG   +GG+++G+ R L  K      + + N+    I  LVIIGG  +  G     Q    Y              ++ C  + IV +  ++ N+  G+D +IG ++AL  I    D +  + A + +R F++E MG +CGYLA +  +++ A   +I  EK    + E ++   LE+ K++ +R  L++ +E      N      +       +   D R  VLGH+Q+GG PT FDR  A + GA+++  L +  +          N    ACV+ + K  +  +PL D  + T
Sbjct:  403 NVAVMNIGAPCAGMNAAVRSAVKMGIIQGHSMLAVHDGFDGLAHG--QIEPITWTSVSGWTGMGGSMLGTKRTLPGKMME---EVSLNIAKYNIHALVIIGGFEAYVGGLELVQAREKY--------------EEVC--IPIVVIPATVSNNVPGSDFSIGADTALNTITSTCDRIKQSAAGTKRRVFIVETMGGYCGYLATMAGLAAGADAAYIFEEKFTIRDLEANVEHLLEKMKTTVKR-GLILRNENC--NSNYTTDFIFSLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKSVLWLTEKLKECYRHGRIFANTPDSACVLGMRKRALNFQPLADLKADT 731

HSP 4 Score: 72.4034 bits (176), Expect = 1.211e-12
Identity = 56/204 (27.45%), Postives = 97/204 (47.55%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE-------------EVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLSQDTSANV 2246
            +V +  +I N+  G    +G+D+ ++ I+  +D +   +   Q R +I E +G +CGYL  + ALA GAD  +I E              +++  S    +N++       +  G + R+     P  S  I KL     +  FD R   +GHV  GG  S+FDR LG+R G+EA++    +LE   +    ++ LS + +  +
Sbjct:  160 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAITTTAQSHQ-RTFILEVMGRHCGYLALVSALACGADWVFIPEMPPDEGWEDHLCRRLMDQRSRGSRLNVII------VAEGAISRDG---KPITSDQIKKLVTD--RLGFDTRTTVLGHVQRGGTPSAFDRILGSRMGVEAVM---ALLEATPDTPACVVSLSGNQAVRL 348

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Match: pfkmb (ATP-dependent 6-phosphofructokinase, muscle type [Source:NCBI gene;Acc:101174339])

HSP 1 Score: 474.552 bits (1220), Expect = 0.000e+0
Identity = 254/511 (49.71%), Postives = 334/511 (65.36%), Query Frame = 2

Query:   89 QGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +G +IAVLTSGGD+QGMNAAV++ VR+GLY GA VYFV EG QGL+DGG +IR A W  VS M+ LGGTVIGSARC DF+++ GR KAA NL+   I NL +IGGDGSLTGAN F+ EW   + +LI+   I + + K   +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+E  DA+ TTA SHQR F++EVMGRHCGYLAL+TA++  A +VFIPE PP + WED LC++L E +    R+N++I++EGAID H +PIT   +K ++  ++  DTR T+LGHVQRGG P++FDRILA R G EA+ AL++A   P  PACV+ +  N  VR PLM+CV  T D+   M  G F + V +RG SF  N+  Y+ L  + P   K                      ++  I I+  G    GMNA +R+ VR+ +      + +   FDGL    I P+ W+ V G    GGSI+GT R LP +   E ++  + +F +  L+IIGGFE
Sbjct:   14 EGRAIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVYFVHEGYQGLVDGGDHIRPATWESVSMMLQLGGTVIGSARCQDFRSKEGRTKAAYNLVKLGITNLCVIGGDGSLTGANQFRTEWKDLLADLIKAGKITANEAKNSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFILEVMGRHCGYLALVTALACGADWVFIPEMPPEEGWEDHLCRRLTEQRGRGSRLNIIIVAEGAIDRHGKPITCDLVKQLVSKKLGFDTRTTILGHVQRGGTPSAFDRILASRMGVEAVMALLEA--TPDTPACVVSLSGNMAVRLPLMECVQVTKDVMTAMAEGRFDEAVNLRGKSFENNWNTYKMLAHVHPPETK----------------------SNINIAILNVGAPCAGMNAAVRAAVRIGLLQGHQMLAVHDSFDGLAHGMIEPITWSGVAGWTGKGGSILGTKRSLPHEF-MEEISLSITKFNIHGLVIIGGFE 499

HSP 2 Score: 220.705 bits (561), Expect = 0.000e+0
Identity = 104/261 (39.85%), Postives = 159/261 (60.92%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY---------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETS 2372
            L G E+ + ++KY+   IP+V++PAT++NN+PG  F++G+DT +N I  T D +K  + GT+ R++I ET+G  CGYL TM  LA GAD +YI EE  N+  L  +V  L  K+   +K GL+LRN   +  Y + +IF L+ +EGKGIFDCR N +GH+  GG  S FDRN GT+ G+++++W+ D L+             DS  +LG+ +       P+    +  D+E   P  QWW+KLRP+L+I+  ++   + S
Sbjct:  502 LGGLEMVQAREKYEELCIPLVVVPATVSNNVPGTDFSIGADTALNTITMTCDRIKQSAAGTKRRVFIVETMGGFCGYLATMAGLASGADAAYIYEESFNIHHLEMNVEHLVEKMKTTVKRGLILRNEKCNANYTTDFIFNLYSEEGKGIFDCRKNVLGHMQQGGTPSPFDRNFGTKMGIKSVLWLTDKLKECYRHGRIFANSQDSACVLGMRKRALV-FQPLAELKDETDFEHRIPKKQWWLKLRPILKILAKYKINLDIS 761

HSP 3 Score: 113.235 bits (282), Expect = 2.330e-25
Identity = 100/359 (27.86%), Postives = 166/359 (46.24%), Query Frame = 2

Query:   98 SIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALI-TTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXX------XXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQ----------NPSIPACVICIVKNEIVRKPLMDCVSKT 1120
            +IA+L  G    GMNAAV++ VR+GL  G  +  V +   GL  G   I    WS V+G    GG+++G+ R L  +      + + ++    I  LVIIGG  +  G     Q    Y              ++ C  L +V    ++ N+  GTD +IG ++AL  I    D +  + A + +R F++E MG  CGYLA +  ++S A   +I E+  N  + E ++   +E+ K++ +R          + + N+   A Y  D I +    + +        VLGH+Q+GG P+ FDR    + G +++  L D  +          N    ACV+ + K  +V +PL +   +T
Sbjct:  401 NIAILNVGAPCAGMNAAVRAAVRIGLLQGHQMLAVHDSFDGLAHG--MIEPITWSGVAGWTGKGGSILGTKRSLPHEFME---EISLSITKFNIHGLVIIGGFEAFLGGLEMVQAREKY--------------EELCIPLVVV--PATVSNNVPGTDFSIGADTALNTITMTCDRIKQSAAGTKRRVFIVETMGGFCGYLATMAGLASGADAAYIYEESFNIHHLEMNVEHLVEKMKTTVKR---------GLILRNEKCNANYTTDFIFNLYSEEGKGIFDCRKNVLGHMQQGGTPSPFDRNFGTKMGIKSVLWLTDKLKECYRHGRIFANSQDSACVLGMRKRALVFQPLAELKDET 729

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Match: pfkla (phosphofructokinase, liver type [Source:NCBI gene;Acc:101169572])

HSP 1 Score: 473.396 bits (1217), Expect = 0.000e+0
Identity = 254/510 (49.80%), Postives = 344/510 (67.45%), Query Frame = 2

Query:   92 GHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            G ++AVLTSGGD+QGMNAAV++V RMG+Y GA VY + EG QGL+DGG NI+ A+W  V+ ++ LGGT+IGSARC  F TR GRL AA NL+ + I NL + GGDGSLTGANIF+ EW   +DEL+++  I  +  +   +LNIVGLVGSIDNDFCGTDMTIG +SAL RIME  DA++TTA+SHQR FV+EVMGRHCGYLAL++A++S A ++FIPE PP + WED +C +LE +++   R+N+VI++EGA D++ +PI++ YIKD++  R+  DTR+TVLGHVQRGG P++FDRIL+ + G EA+ AL++A   P  PACVI +  N  VR PLM+CV  T  +Q  M    F + V +RG SF  N+ IY+ L    P                        S ++  + ++  G  A GMNA +RS VR+A+A+     G+  GF GL    I  + W +V G    GGS++GT R LP +   E +   + +F +SAL++IGGFE
Sbjct:   15 GRAMAVLTSGGDAQGMNAAVRAVTRMGIYVGAKVYLIYEGYQGLVDGGDNIKLAHWHSVTNIIQLGGTIIGSARCKAFTTREGRLAAAFNLVKKGITNLCVCGGDGSLTGANIFRNEWRGLLDELVKKGRITDVLAEKHNHLNIVGLVGSIDNDFCGTDMTIGADSALHRIMEIIDAIMTTANSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPESPPLEGWEDRMCARLERSRTKGSRLNIVILAEGATDVNGKPISSSYIKDLVTKRMGYDTRVTVLGHVQRGGTPSAFDRILSSKLGVEAVIALMEA--TPDTPACVIGLSGNHAVRLPLMECVEMTKLVQKAMNEKRFDEAVKLRGGSFENNWNIYKLLAFQKPAL----------------------SESNFSLAVLNVGAPAAGMNAAVRSAVRLALAHGHKVYGVHDGFQGLANGEIFEMKWRNVAGWTGQGGSLLGTKRTLP-ETDMEKIVENIAKFNISALLVIGGFE 499

HSP 2 Score: 207.223 bits (526), Expect = 0.000e+0
Identity = 100/259 (38.61%), Postives = 156/259 (60.23%), Query Frame = 3

Query: 1626 QELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY---------DNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSF 2375
            Q+L+  +  YD   IP+ ++PATI+NN+PG  F++G+DT VN  +   D +K  + GT+ R+++ ET+G  CGYL T   +A GAD +YI EE  N+  L  +V  L  K+  +I+ GLVLRN   H  Y + +I++L+  EGKG+FDCR N +GH+  GG  S FDRN GT+ G++A+ W+ + L              ++  +LG+++   A+ IP+       D+E   P  QWW  LRPLL+++    ++Y+T+F
Sbjct:  505 QQLYDARSHYDELCIPMCVVPATISNNVPGTDFSLGADTAVNAAMEGCDKIKQSASGTKRRVFVVETMGGYCGYLATSTGIAVGADAAYIFEEQFNIHDLKANVEHLAEKMKKDIQRGLVLRNEKCHENYSTDFIYRLYSAEGKGVFDCRINVLGHLQQGGSPSPFDRNFGTKLGVKAVQWITERLTECFRQGRVFANSPETACVLGINRKI-ASFIPVTELKALTDFEHRMPNVQWWANLRPLLKML----ARYQTNF 758

HSP 3 Score: 124.405 bits (311), Expect = 6.652e-29
Identity = 100/319 (31.35%), Postives = 155/319 (48.59%), Query Frame = 2

Query:   74 PAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAH-SHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALID 1027
            PA      S+AVL  G  + GMNAAV+S VR+ L  G  VY V +G QGL +G   I E  W +V+G    GG+++G+ R L     +   K  +N+    I  L++IGG     G        + Y DEL             C  + +V    +I N+  GTD ++G ++A+   ME  D +  +A  + +R FV+E MG +CGYLA  T I+  A   +I E+  N +      + L E    + +  LV+ +E   + ++      Y       +   D RI VLGH+Q+GG+P+ FDR    + G +A+  + +
Sbjct:  393 PALSESNFSLAVLNVGAPAAGMNAAVRSAVRLALAHGHKVYGVHDGFQGLANG--EIFEMKWRNVAGWTGQGGSLLGTKRTL---PETDMEKIVENIAKFNISALLVIGGFEGFEGVQQLYDARSHY-DEL-------------CIPMCVV--PATISNNVPGTDFSLGADTAVNAAMEGCDKIKQSASGTKRRVFVVETMGGYCGYLATSTGIAVGADAAYIFEEQFNIHDLKANVEHLAEKMKKDIQRGLVLRNEKCHENYSTDFI--YRLYSAEGKGVFDCRINVLGHLQQGGSPSPFDRNFGTKLGVKAVQWITE 688

HSP 4 Score: 71.2478 bits (173), Expect = 2.881e-12
Identity = 56/193 (29.02%), Postives = 95/193 (49.22%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLR--------NNVSHHPYDSHYIFKLF-KQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVILGLS 2225
            +V +  +I N+  G    +G+D+ ++ I+  +D + + +  +  R ++ E +G +CGYL  + ALA GAD  +I E       L    + + A+L      G  L          +V+  P  S YI  L  K+ G   +D R   +GHV  GG  S+FDR L ++ G+EA+I    ++E   +    ++GLS
Sbjct:  158 IVGLVGSIDNDFCGTDMTIGADSALHRIMEIIDAI-MTTANSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPES----PPLEGWEDRMCARLERSRTKGSRLNIVILAEGATDVNGKPISSSYIKDLVTKRMG---YDTRVTVLGHVQRGGTPSAFDRILSSKLGVEAVI---ALMEATPDTPACVIGLS 339

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Match: SMESG000074512.1 (SMESG000074512.1)

HSP 1 Score: 1003.05 bits (2592), Expect = 0.000e+0
Identity = 523/537 (97.39%), Postives = 524/537 (97.58%), Query Frame = 2

Query:   11 EFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            +FSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQI NLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVKIDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISK            TDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE
Sbjct:  127 QFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQISNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVKIDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISK------------TDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 651

HSP 2 Score: 400.208 bits (1027), Expect = 6.961e-127
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY 2192
            LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY
Sbjct:  654 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY 845

HSP 3 Score: 57.3806 bits (137), Expect = 3.745e-8
Identity = 48/184 (26.09%), Postives = 84/184 (45.65%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVI 2213
            +V +  +I N+  G    +G ++ +  I+   D L + +  +  R ++ E +G +CGYL  + A++  A   +I E+  N    +     L+       +  LV+ +    ++ + P  + YI  +     K   D R   +GHV  GG  +SFDR L  R G EAI  + D  +N    + VI
Sbjct:  297 IVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVK--IDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVI 477

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Match: SMESG000074512.1 (SMESG000074512.1)

HSP 1 Score: 1002.28 bits (2590), Expect = 0.000e+0
Identity = 524/540 (97.04%), Postives = 526/540 (97.41%), Query Frame = 2

Query:    2 MYKEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            ++ EFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQI NLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVKIDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISK            TDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE
Sbjct:   80 VHDEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQISNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVKIDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISK------------TDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 607

HSP 2 Score: 399.438 bits (1025), Expect = 5.852e-127
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY 2192
            LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY
Sbjct:  610 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY 801

HSP 3 Score: 56.6102 bits (135), Expect = 6.908e-8
Identity = 48/184 (26.09%), Postives = 84/184 (45.65%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVI 2213
            +V +  +I N+  G    +G ++ +  I+   D L + +  +  R ++ E +G +CGYL  + A++  A   +I E+  N    +     L+       +  LV+ +    ++ + P  + YI  +     K   D R   +GHV  GG  +SFDR L  R G EAI  + D  +N    + VI
Sbjct:  253 IVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIIIDRVK--IDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVI 433

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Match: SMESG000029088.1 (SMESG000029088.1)

HSP 1 Score: 534.258 bits (1375), Expect = 0.000e+0
Identity = 288/521 (55.28%), Postives = 370/521 (71.02%), Query Frame = 2

Query:   59 EGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            EGK+I    F+G SIAVLTSGGDSQGMN+AV++VVRMG+YCG++V+F+ EG QGL DGG NI EA W+DVSG++ LGGTVIGSARC+DFKTR G L AAKNLIDR I NLV+IGGDGSLTGAN F+ EW S + EL+E+  I   + + C +LNIVG+VGSIDNDFCGTDMTIG +SAL RI+EA DA+ +TA SHQRCF++EVMGRHCGYLAL+ A++SEA++VFIPE PP+ +WE  LC+KL+  + + +RVN++++SEGAID++  PIT+  IK++I  ++ IDTR+TVLGHVQRGGNP++FDRIL +R GAEA+ AL+DA  N +  ACVI I  N+ VR PL+ CV KT  +   M    F + V +RG SF  N + Y  L+ L P                  K  L+      RI I   G  ACG+NA +RS VR+ I      IG+  GF GL       L WN VRG   +GGS++GT R LPS++GF++VA    E +LS +++IGGFE
Sbjct:   25 EGKYIDKDCFKGKSIAVLTSGGDSQGMNSAVRAVVRMGIYCGSSVFFIKEGYQGLCDGGKNIVEATWADVSGIMQLGGTVIGSARCMDFKTREGLLNAAKNLIDRNINNLVVIGGDGSLTGANYFRLEWNSLLKELLEQNRITQGQIEKCSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEACDAISSTAQSHQRCFIMEVMGRHCGYLALVAALASEAEWVFIPEMPPSLDWEAKLCQKLKINRDNGQRVNIILVSEGAIDINCNPITSDQIKNLITSQLNIDTRVTVLGHVQRGGNPSAFDRILGIRMGAEAVLALMDAEHNLNSEACVISIDGNQTVRVPLLRCVEKTRAVGEAMNAKNFEEAVKLRGRSFTNNLETYIKLSKLQPP-----------------KTLLN---IHKRIAIFNVGAPACGINATMRSFVRLMITKGYEIIGVHGGFKGLIDDDKEDLTWNRVRGWAGLGGSMLGTRRHLPSEVGFDIVAKYFRELRLSGILVIGGFE 525

HSP 2 Score: 230.72 bits (587), Expect = 0.000e+0
Identity = 107/261 (41.00%), Postives = 172/261 (65.90%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENY---------DNDSIVILGLSQDTSANVIPIHTQF-ENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIM 2381
            EL + + +Y  F+IP+++IPATI+NNIPG  F++G+DT++N I   +D +K  ++GT+ R+++ ET G  CGYL TM  LAGGAD +YI EE   ++ L +DV+ ++AK+ D ++ GL+L N  ++  Y + ++++L+ +EGKGIFD R+N  GH+  GG+ + FDRN+GT+ G +A  W+ + +EN           ND+I +LGL++       P+   F    D++   P++QWW+ LRPL+RI+  H+S YE+  IM
Sbjct:  532 ELNEKRQEYPEFQIPILVIPATISNNIPGTDFSLGADTSLNEITRIVDKIKQSAVGTKRRVFVIETQGGYCGYLATMSGLAGGADAAYIHEEEFGIDDLRKDVDHMRAKIEDNVQRGLILINEKANVNYSTDFVYRLYSEEGKGIFDTRYNIPGHIQQGGVPTPFDRNMGTKMGAKASDWLSEQIENSANESGVKTNSNDTICLLGLTK-RQMTFTPVSELFIRETDFKHRIPINQWWLNLRPLMRIMAKHQSLYESESIM 791

HSP 3 Score: 101.293 bits (251), Expect = 1.042e-21
Identity = 99/392 (25.26%), Postives = 184/392 (46.94%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDG---SLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSS---NRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPA----------CVICIVKNEIVRKPLMDCVSKTLDIQNLMR-NGIFVDL-----VMMRGPSFYRNYKI 1207
            IA+   G  + G+NA ++S VR+ +  G  +  V  G +GLID   +  +  W+ V G   LGG+++G+ R L   +  G    AK   + ++  +++IGG     S+   N  +QE+  +                    + I+ +  +I N+  GTD ++G +++L  I    D +  +A  + +R FVIE  G +CGYLA ++ ++  A   +I E+   +   DDL K ++  ++    N +  L++I+E A    N      Y       +   DTR  + GH+Q+GG PT FDR +  + GA+A   L +  +N +  +          C++ + K ++   P+ +   +  D ++ +  N  +++L     +M +  S Y +  I
Sbjct:  427 IAIFNVGAPACGINATMRSFVRLMITKGYEIIGVHGGFKGLIDD--DKEDLTWNRVRGWAGLGGSMLGTRRHL--PSEVGFDIVAKYFRELRLSGILVIGGFEAFISVCELNEKRQEYPEF-------------------QIPILVIPATISNNIPGTDFSLGADTSLNEITRIVDKIKQSAVGTKRRVFVIETQGGYCGYLATMSGLAGGADAAYIHEE---EFGIDDLRKDVDHMRAKIEDNVQRGLILINEKA--NVNYSTDFVYRLYSEEGKGIFDTRYNIPGHIQQGGVPTPFDRNMGTKMGAKASDWLSEQIENSANESGVKTNSNDTICLLGLTKRQMTFTPVSELFIRETDFKHRIPINQWWLNLRPLMRIMAKHQSLYESESI 790

HSP 4 Score: 71.2478 bits (173), Expect = 2.082e-12
Identity = 51/184 (27.72%), Postives = 92/184 (50.00%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRN----NVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVI 2213
            +V +  +I N+  G    +G+D+ ++ I+   D +   +  +  R +I E +G +CGYL  + ALA  A+  +I E   +L+   +    LK    +  +  ++L +    +++ +P  S  I  L   +     D R   +GHV  GG  S+FDR LG R G EA++ + D   N ++++ VI
Sbjct:  179 IVGMVGSIDNDFCGTDMTIGTDSALHRIIEACDAIS-STAQSHQRCFIMEVMGRHCGYLALVAALASEAEWVFIPEMPPSLDWEAKLCQKLKINRDNGQRVNIILVSEGAIDINCNPITSDQIKNLITSQLN--IDTRVTVLGHVQRGGNPSAFDRILGIRMGAEAVLALMDAEHNLNSEACVI 359

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Match: SMESG000028432.1 (SMESG000028432.1)

HSP 1 Score: 487.649 bits (1254), Expect = 0.000e+0
Identity = 260/521 (49.90%), Postives = 352/521 (67.56%), Query Frame = 2

Query:   59 EGKFIPAGGFQGHSIAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXXXXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIPACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTVACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            EG +I    FQG  IAVLTSGGDSQGMN+ +++VVRMG+YCG +VY + EG QGL+ G   I EA W+ VS ++ LGGT+IGS  C +F TR GR  +AKNLI   I NLVIIGGDGSLTGAN+FK EW+  I+EL++   I + +     +LN+VG+V S+DND CGTDMTIG +SAL RI+EA DA+ +TAHSHQRCF++E+MGR CGYLAL+TA++SEA++VFIPE PP++ WE+ LC+KL+  K + +RVN+++ISEGAID+H +PIT+  ++ II  ++  D+R+TVLGHVQRGGNP++FDRIL +R GAEA+ AL+D  +NP + +C+I +  N+IVR PL  CV KT  I+  +R   F   V +RG SF  N   Y+ L                 SK S  K    NS    +I I+  G  ACG NA++RS  R  I+     IG+  GF G  K     L WN V+G + +GGSI+G  R  P  +G ++++  +   ++S ++IIGGFE
Sbjct:   21 EGMYIHKDCFQGKKIAVLTSGGDSQGMNSVLRAVVRMGIYCGCSVYLIKEGFQGLVAGKDYIVEATWASVSNILQLGGTIIGSTECEEFMTRDGRKLSAKNLIIAGITNLVIIGGDGSLTGANLFKTEWSELIEELVKDGQISTSEYLSSVSLNVVGVVASVDNDVCGTDMTIGTDSALHRIIEACDAITSTAHSHQRCFILEIMGRQCGYLALVTALASEAEWVFIPESPPDEGWEEKLCEKLDRNKHNGQRVNIIVISEGAIDLHGKPITSSMVQQIITSKLNFDSRVTVLGHVQRGGNPSAFDRILGIRMGAEAVLALMDGDKNPELESCIISLHGNQIVRVPLKTCVEKTKAIEEALRIQNFQRAVNLRGRSFVNNLNAYKNL-----------------SKSSPPKSLEKNS---KKIAILNVGAPACGTNAVVRSFTRFMISKGFDVIGVHEGFQGFVKGDYETLDWNKVKGWVGIGGSILGIRRETPENVGMKLISKIIDRVQISGMLIIGGFE 521

HSP 2 Score: 213.386 bits (542), Expect = 0.000e+0
Identity = 102/255 (40.00%), Postives = 160/255 (62.75%), Query Frame = 3

Query: 1629 ELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYD-NDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQWWMKLRPLLRIIQTHESQYETSFIMRHL 2390
            +++K ++ Y    +P++ +PATI+NN+PG  F++G+DT++N I   +D +K  ++GT+ R++I ET G +CGYL TM  +AGGAD +YI EE   ++ L  DV  LKAK+ D+++ GL+L N  ++  Y + ++ +LF++EGKGIFD R N  GH+  GG  S FDRN+G + G +A  W     E  + + +  +LG+ Q       P+      +D++   P +QWW+ LRPLLRI+  HES Y +  IM  L
Sbjct:  528 QMYKARNTYPNLNMPMLCVPATISNNVPGTDFSLGADTSLNEITEIVDKIKQSAVGTKRRVFIVETHGGHCGYLATMSGIAGGADAAYIHEEEFGIDELRADVTHLKAKIADKVQRGLILINEKANKNYSTQFVHRLFEEEGKGIFDARMNVPGHLQQGGNPSPFDRNMGFKMGSKASDWFTQQFEEPEKHPNSCLLGI-QRWEHKFTPVWELEPISDFKHRLPCEQWWLNLRPLLRIMAKHESLYTSESIMTGL 781

HSP 3 Score: 108.997 bits (271), Expect = 3.829e-24
Identity = 105/392 (26.79%), Postives = 183/392 (46.68%), Query Frame = 2

Query:  101 IAVLTSGGDSQGMNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDR-QIFNLVIIGGDGSLTGANIFKQEWTSYIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDALITTA-HSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN-DNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYXXXXXXXXXX--XXXXXXVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIP--ACVICIVKNEIVRKPLMD------------CVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNYKIYEAL 1219
            IA+L  G  + G NA V+S  R  +  G  V  V EG QG + G     +  W+ V G V +GG+++G  R  +     G +K    +IDR QI  ++IIGG           + +TS +     R    ++      N+ ++ +  +I N+  GTD ++G +++L  I E  D +  +A  + +R F++E  G HCGYLA ++ I+  A   +I E+    D    D+   L+   +   +  L++I+E A    N+  + Q++  +  +  K   D R+ V GH+Q+GGNP+ FDR +  + G++A        + P     +C++ I + E    P+ +            C    L+++ L+R       +M +  S Y +  I   L
Sbjct:  423 IAILNVGAPACGTNAVVRSFTRFMISKGFDVIGVHEGFQGFVKGDYETLD--WNKVKGWVGIGGSILGIRR--ETPENVG-MKLISKIIDRVQISGMLIIGG----------FEAYTSLLQMYKARNTYPNL------NMPMLCVPATISNNVPGTDFSLGADTSLNEITEIVDKIKQSAVGTKRRVFIVETHGGHCGYLATMSGIAGGADAAYIHEEEFGIDELRADVT-HLKAKIADKVQRGLILINEKA----NKNYSTQFVHRLFEEEGKGIFDARMNVPGHLQQGGNPSPFDRNMGFKMGSKASDWFTQQFEEPEKHPNSCLLGIQRWEHKFTPVWELEPISDFKHRLPCEQWWLNLRPLLR-------IMAKHESLYTSESIMTGL 781

HSP 4 Score: 68.9366 bits (167), Expect = 1.218e-11
Identity = 57/193 (29.53%), Postives = 93/193 (48.19%), Query Frame = 3

Query: 1674 VVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDE------------EVLNLESLN-QDVNILKAKLFDEIKYGLVLRNNVSHHPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLEAIIWVKDMLENYDNDSIVI 2213
            VV + A++ N++ G    +G+D+ ++ I+   D +   +  +  R +I E +G  CGYL  + ALA  A+  +I E            E L+    N Q VNI+       I  G +   ++   P  S  + ++     K  FD R   +GHV  GG  S+FDR LG R G EA++ + D  +N + +S +I
Sbjct:  175 VVGVVASVDNDVCGTDMTIGTDSALHRIIEACDAIT-STAHSHQRCFILEIMGRQCGYLALVTALASEAEWVFIPESPPDEGWEEKLCEKLDRNKHNGQRVNIIV------ISEGAI---DLHGKPITSSMVQQIITS--KLNFDSRVTVLGHVQRGGNPSAFDRILGIRMGAEAVLALMDGDKNPELESCII 355

[Close]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Match: SMESG000074570.1 (SMESG000074570.1)

HSP 1 Score: 233.417 bits (594), Expect = 8.888e-100
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 3

Query: 1617 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLF 1961
            LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLF
Sbjct:   90 LSGQELWKNKDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLNLESLNQDVNILKAKLF 204

HSP 2 Score: 151.369 bits (381), Expect = 8.888e-100
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 2

Query: 1361 MNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRXXXXXXXXXXXTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 1621
            MNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE
Sbjct:    1 MNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIISMGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFE 87

HSP 3 Score: 70.4774 bits (171), Expect = 1.994e-13
Identity = 56/210 (26.67%), Postives = 101/210 (48.10%), Query Frame = 2

Query:  137 MNAAVKSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVIGSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGG-DGSLTGANIFKQEWTSYIDELIERKVIDSIKKKY-CKNLNIVGLVGSIDNDFCGTDMTIGVNSALTRIMEATDAL-ITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKPPN 757
            MNA ++SVVRM +        +  G  GL      I    W+DV G++++GG++IG+ R L   ++ G    A  L++ ++  L+IIGG +  L+G  ++K                   K KY    + +V +  +I N+  G    +G ++ +  I+   D L + +  +  R ++ E +G +CGYL  + A++  A   +I E+  N
Sbjct:    1 MNAIIRSVVRMAIANDLYPIGIKYGFDGL--NKKTITPLLWNDVRGIISMGGSIIGTTRVL--PSQIGFEVVASRLLEFKLSALIIIGGFEAFLSGQELWKN------------------KDKYDIFRIPVVIIPATIANNIPGITFAVGSDTTVNVIVNTLDNLKLGSIGTQYRIYITETLGTNCGYLNTMGALAGGADMSYIDEEVLN 188

The following BLAST results are available for this feature:

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Human
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Celegans
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Yeast
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Nematostella
BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
PFKP	8.761e-25	50.48	phosphofructokinase, platelet [Source:HGNC Symbol;...	[more]
PFKM	3.424e-29	48.77	phosphofructokinase, muscle [Source:HGNC Symbol;Ac...	[more]
PFKM	2.658e-29	49.80	phosphofructokinase, muscle [Source:HGNC Symbol;Ac...	[more]
PFKM	2.658e-29	49.80	phosphofructokinase, muscle [Source:HGNC Symbol;Ac...	[more]
PFKM	2.658e-29	49.80	phosphofructokinase, muscle [Source:HGNC Symbol;Ac...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
pfk-1.1	3.307e-180	46.74	ATP-dependent 6-phosphofructokinase 1 [Source:Uni...	[more]
pfk-1.2	3.701e-121	39.69	ATP-dependent 6-phosphofructokinase 2 [Source:Uni...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Pfk	5.575e-32	51.83	gene:FBgn0003071 transcript:FBtr0088421	[more]
Pfk	4.514e-32	51.64	gene:FBgn0003071 transcript:FBtr0088422	[more]
Pfk	6.827e-32	51.94	gene:FBgn0003071 transcript:FBtr0088420	[more]
Pfk	8.059e-32	51.94	gene:FBgn0003071 transcript:FBtr0304925	[more]
Pfk	6.288e-169	51.57	gene:FBgn0003071 transcript:FBtr0333067	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pfkpa	5.601e-27	50.78	phosphofructokinase, platelet a [Source:ZFIN;Acc:Z...	[more]
pfkpa	7.534e-27	50.78	phosphofructokinase, platelet a [Source:ZFIN;Acc:Z...	[more]
pfkpa	8.357e-28	50.78	phosphofructokinase, platelet a [Source:ZFIN;Acc:Z...	[more]
pfkpb	2.031e-27	49.90	phosphofructokinase, platelet b [Source:ZFIN;Acc:Z...	[more]
pfkma	1.635e-25	49.61	phosphofructokinase, muscle a [Source:ZFIN;Acc:ZDB...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pfkp	1.291e-23	50.58	phosphofructokinase, platelet [Source:NCBI gene;Ac...	[more]
pfkp	8.250e-24	49.71	phosphofructokinase, platelet [Source:NCBI gene;Ac...	[more]
ENSXETT00000054028.1	2.770e-24	49.91	phosphofructokinase, muscle [Source:NCBI gene;Acc:...	[more]
pfkp	4.723e-22	49.71	phosphofructokinase, platelet [Source:NCBI gene;Ac...	[more]
pfkp	3.725e-26	49.71	phosphofructokinase, platelet [Source:NCBI gene;Ac...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Pfkp	4.127e-27	50.00	phosphofructokinase, platelet [Source:MGI Symbol;A...	[more]
Pfkl	3.514e-31	50.29	phosphofructokinase, liver, B-type [Source:MGI Sym...	[more]
Pfkp	1.392e-28	50.00	phosphofructokinase, platelet [Source:MGI Symbol;A...	[more]
Pfkm	2.779e-29	49.61	phosphofructokinase, muscle [Source:MGI Symbol;Acc...	[more]
Pfkm	2.779e-29	49.61	phosphofructokinase, muscle [Source:MGI Symbol;Acc...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q27778\|PFKA_SCHMA	8.382e-23	53.55	ATP-dependent 6-phosphofructokinase OS=Schistosoma...	[more]
sp\|P52034\|PFKA_DROME	4.525e-31	51.64	ATP-dependent 6-phosphofructokinase OS=Drosophila ...	[more]
sp\|Q01813\|PFKAP_HUMAN	4.207e-24	50.48	ATP-dependent 6-phosphofructokinase, platelet type...	[more]
sp\|Q5R636\|PFKAP_PONAB	4.356e-24	50.29	ATP-dependent 6-phosphofructokinase, platelet type...	[more]
sp\|P47860\|PFKAP_RAT	1.101e-24	50.78	ATP-dependent 6-phosphofructokinase, platelet type...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
G4VKA5	3.498e-21	53.33	ATP-dependent 6-phosphofructokinase OS=Schistosoma...	[more]
G4VKA6	3.596e-21	53.33	ATP-dependent 6-phosphofructokinase OS=Schistosoma...	[more]
A0A3Q0KF43	3.086e-21	53.33	ATP-dependent 6-phosphofructokinase OS=Schistosoma...	[more]
A0A158QJ34	6.981e-21	52.21	ATP-dependent 6-phosphofructokinase OS=Rodentolepi...	[more]
A0A0B6Z7M5	9.010e-17	53.64	ATP-dependent 6-phosphofructokinase OS=Arion vulga...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pfklb	1.211e-28	50.29	ATP-dependent 6-phosphofructokinase, liver type [S...	[more]
pfkma	2.196e-26	49.90	ATP-dependent 6-phosphofructokinase, muscle type-l...	[more]
pfkpb	2.747e-26	49.13	ATP-dependent 6-phosphofructokinase, platelet type...	[more]
pfkmb	3.175e-25	49.61	phosphofructokinase, muscle b [Source:ZFIN;Acc:ZDB...	[more]
pfkla	2.638e-30	49.61	ATP-dependent 6-phosphofructokinase, liver type-li...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000007452.1	8.316e-175	48.95	pep scaffold:Pmarinus_7.0:GL477249:14695:58282:1 g...	[more]
ENSPMAT00000008968.1	3.592e-154	50.10	pep scaffold:Pmarinus_7.0:GL478229:3907:42159:-1 g...	[more]
ENSPMAT00000005911.1	3.290e-144	44.07	pep scaffold:Pmarinus_7.0:GL478489:7235:45113:1 ge...	[more]
ENSPMAT00000008974.1	3.344e-127	48.65	pep scaffold:Pmarinus_7.0:GL478229:3949:42166:-1 g...	[more]
ENSPMAT00000007362.1	2.384e-78	60.38	pep scaffold:Pmarinus_7.0:GL493177:1250:4583:-1 ge...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
PFK2	4.998e-128	39.73	Beta subunit of heterooctameric phosphofructokinas...	[more]
PFK1	1.277e-119	40.35	Alpha subunit of heterooctameric phosphofructokina...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of ATP-dependent 6-phosphofructokinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pfkpa	3.167e-29	49.02	ATP-dependent 6-phosphofructokinase, platelet type...	[more]
pfkpa	3.085e-29	49.22	ATP-dependent 6-phosphofructokinase, platelet type...	[more]
pfkma	2.292e-27	50.78	ATP-dependent 6-phosphofructokinase, muscle type [...	[more]
pfkmb	2.330e-25	49.71	ATP-dependent 6-phosphofructokinase, muscle type [...	[more]
pfkla	6.652e-29	49.80	phosphofructokinase, liver type [Source:NCBI gene;...	[more]

BLAST of ATP-dependent 6-phosphofructokinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000074512.1	6.961e-127	97.39	SMESG000074512.1	[more]
SMESG000074512.1	5.852e-127	97.04	SMESG000074512.1	[more]
SMESG000029088.1	1.042e-21	55.28	SMESG000029088.1	[more]
SMESG000028432.1	3.829e-24	49.90	SMESG000028432.1	[more]
SMESG000074570.1	8.888e-100	100.00	SMESG000074570.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30026981 ID=SMED30026981|Name=ATP-dependent 6-phosphofructokinase|organism=Schmidtea mediterranea sexual|type=transcript|length=2390bp
TATGTATAAAGAATTTTCATTACAAACTGACAATGAAGAATTTCTTTTGA
AATCTAAGGAGGGAAAGTTTATTCCGGCCGGAGGGTTTCAAGGACACTCA
ATTGCGGTTTTAACGAGTGGCGGGGATTCTCAAGGAATGAATGCAGCAGT
AAAATCAGTAGTTCGAATGGGTCTTTATTGTGGTGCGACGGTTTACTTTG
TTTTGGAAGGATTACAAGGATTAATTGACGGTGGTCTTAACATAAGGGAA
GCATATTGGTCTGACGTTTCCGGAATGGTTAATTTGGGTGGTACCGTAAT
AGGTAGTGCAAGGTGCTTGGATTTTAAAACTAGATCTGGAAGGTTAAAAG
CTGCGAAAAATTTAATTGACAGACAAATATTCAATCTTGTGATAATAGGA
GGAGACGGGTCACTTACCGGAGCTAATATTTTCAAACAAGAATGGACCTC
TTACATCGATGAATTAATTGAAAGAAAAGTAATCGATTCAATCAAAAAAA
AGTATTGTAAAAATTTGAATATTGTCGGTTTGGTCGGGTCCATAGATAAC
GATTTTTGTGGCACTGACATGACAATTGGAGTCAATTCTGCTCTCACTAG
AATAATGGAGGCTACTGATGCTCTCATAACTACTGCACATAGCCATCAAA
GATGTTTCGTCATTGAAGTTATGGGTCGACATTGTGGATATCTGGCATTG
ATAACCGCAATCAGTTCTGAAGCGCAGTTTGTTTTCATTCCAGAAAAACC
TCCAAATGATAACTGGGAAGATGATCTTTGCAAGAAACTTGAAGAAGCCA
AAAGTTCAAATCGTAGAGTGAATCTCGTGATTATTAGTGAAGGAGCTATA
GATATGCATAACCAACCAATTACTGCTCAATATATAAAGGATATAATTAT
TGATAGGGTGAAAATTGATACTCGAATCACTGTATTGGGCCATGTTCAGC
GAGGCGGAAATCCGACTTCCTTCGACAGAATTCTAGCATTGAGAGAGGGA
GCTGAAGCAATACATGCATTAATTGATGCGGCACAGAATCCATCAATTCC
CGCTTGTGTAATTTGTATCGTAAAAAATGAAATAGTTCGCAAACCCTTAA
TGGACTGCGTCTCAAAAACTTTAGATATTCAAAATCTTATGAGGAATGGA
ATTTTTGTCGATCTTGTTATGATGAGAGGACCATCATTTTACCGGAATTA
TAAAATTTATGAAGCGCTTAATGTGTTAATGCCAAAGCACAGAAAAATGG
TCGGAGAAGGAGCAAGGATTTCAAAGTATTCTGAAAGAAAGCGATTTTTG
GATAATTCTGTAACTGATACAAGAATTGGGATTATGTGTTCTGGTACTGT
TGCCTGCGGAATGAATGCCATCATAAGAAGTGTAGTTCGAATGGCGATCG
CTAATGATCTCTATCCGATCGGTATTAAATATGGGTTTGATGGTCTAAAT
AAAAAAACTATAACACCCTTATTATGGAATGATGTTCGAGGAATAATATC
TATGGGCGGCTCAATAATTGGAACTACGAGAGTCCTCCCATCACAAATTG
GATTTGAGGTTGTGGCAAGCAGATTATTGGAGTTCAAGTTGTCTGCTTTG
ATTATAATAGGAGGCTTTGAGTGGACAAGAATTGTGGAAAAATAAAGATA
AATATGATATATTCCGGATCCCTGTGGTGATAATTCCCGCTACCATAGCC
AATAACATACCAGGAATCACGTTCGCTGTTGGTAGTGACACCACGGTAAA
TGTTATTGTCAATACTTTAGATAATCTTAAACTTGGGAGCATTGGTACCC
AATATAGAATATATATCACAGAAACACTTGGAACCAATTGTGGCTACCTC
AATACTATGGGCGCATTGGCAGGAGGAGCTGATATGTCGTATATAGATGA
AGAAGTGTTAAACCTTGAAAGCCTGAATCAGGATGTAAACATTCTTAAGG
CAAAGCTTTTTGATGAAATTAAATATGGATTGGTGTTGCGTAATAACGTC
AGCCACCACCCTTATGACTCGCATTACATTTTTAAATTATTCAAACAAGA
AGGTAAAGGAATATTTGACTGCAGGCACAATTCAATCGGGCATGTTTCAC
TTGGGGGACTCACTTCATCTTTTGATCGGAATTTGGGCACTCGGTGCGGA
TTAGAAGCAATTATTTGGGTGAAGGATATGTTAGAAAACTATGATAATGA
TTCGATAGTCATACTCGGTTTATCTCAAGATACCTCCGCAAACGTCATCC
CTATTCACACTCAATTTGAAAATGCTGATTATGAAAAATATCGGCCATTG
GACCAATGGTGGATGAAACTAAGGCCGCTATTGCGCATCATACAAACTCA
TGAATCCCAATATGAAACATCTTTTATAATGAGACATCTT

protein sequence of SMED30026981-orf-1

>SMED30026981-orf-1 ID=SMED30026981-orf-1|Name=SMED30026981-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=178bp
MGALAGGADMSYIDEEVLNLESLNQDVNILKAKLFDEIKYGLVLRNNVSH
HPYDSHYIFKLFKQEGKGIFDCRHNSIGHVSLGGLTSSFDRNLGTRCGLE
AIIWVKDMLENYDNDSIVILGLSQDTSANVIPIHTQFENADYEKYRPLDQ
WWMKLRPLLRIIQTHESQYETSFIMRHL

protein sequence of SMED30026981-orf-2

>SMED30026981-orf-2 ID=SMED30026981-orf-2|Name=SMED30026981-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=548bp
MYKEFSLQTDNEEFLLKSKEGKFIPAGGFQGHSIAVLTSGGDSQGMNAAV
KSVVRMGLYCGATVYFVLEGLQGLIDGGLNIREAYWSDVSGMVNLGGTVI
GSARCLDFKTRSGRLKAAKNLIDRQIFNLVIIGGDGSLTGANIFKQEWTS
YIDELIERKVIDSIKKKYCKNLNIVGLVGSIDNDFCGTDMTIGVNSALTR
IMEATDALITTAHSHQRCFVIEVMGRHCGYLALITAISSEAQFVFIPEKP
PNDNWEDDLCKKLEEAKSSNRRVNLVIISEGAIDMHNQPITAQYIKDIII
DRVKIDTRITVLGHVQRGGNPTSFDRILALREGAEAIHALIDAAQNPSIP
ACVICIVKNEIVRKPLMDCVSKTLDIQNLMRNGIFVDLVMMRGPSFYRNY
KIYEALNVLMPKHRKMVGEGARISKYSERKRFLDNSVTDTRIGIMCSGTV
ACGMNAIIRSVVRMAIANDLYPIGIKYGFDGLNKKTITPLLWNDVRGIIS
MGGSIIGTTRVLPSQIGFEVVASRLLEFKLSALIIIGGFEWTRIVEK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002089	reproductive organ
PLANA:0003304	testis primordium

Vocabulary: **INTERPRO**
Term	Definition
IPR035966	PKF_sf
IPR022953	ATP_PFK
IPR000023	Phosphofructokinase_dom
IPR015912	Phosphofructokinase_CS

Vocabulary: **molecular function**
Term	Definition
GO:0003872	6-phosphofructokinase activity
GO:0000166	nucleotide binding
GO:0003824	catalytic activity
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016740	transferase activity
GO:0046872	metal ion binding

Vocabulary: **biological process**
Term	Definition
GO:0006096	glycolytic process
GO:0006002	fructose 6-phosphate metabolic process
GO:0008152	metabolic process
GO:0016310	phosphorylation
GO:0061615	glycolytic process through fructose-6-phosphate

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022953	ATP-dependent 6-phosphofructokinase	PRINTS	PR00476	PHFRCTKINASE	coord: 227..244 score: 44.44 coord: 57..70 score: 45.79 coord: 209..225 score: 71.34 coord: 32..51 score: 73.85 coord: 300..322 score: 66.89 coord: 171..188 score: 73.36 coord: 189..207 score: 44.8 coord: 121..137 score: 64.4 coord: 266..278 score: 39.45
IPR000023	Phosphofructokinase domain	PFAM	PF00365	PFK	coord: 437..541 e-value: 1.2E-24 score: 87.1 coord: 30..335 e-value: 8.7E-104 score: 346.8
None	No IPR available	GENE3D	G3DSA:3.40.50.460		coord: 193..400 e-value: 1.1E-161 score: 539.8
None	No IPR available	GENE3D	G3DSA:3.40.50.450		coord: 423..543 e-value: 3.4E-29 score: 103.7
None	No IPR available	GENE3D	G3DSA:3.40.50.450		coord: 29..375 e-value: 1.1E-161 score: 539.8
None	No IPR available	PANTHER	PTHR13697	PHOSPHOFRUCTOKINASE	coord: 26..536
IPR015912	Phosphofructokinase, conserved site	PROSITE	PS00433	PHOSPHOFRUCTOKINASE	coord: 304..322
IPR035966	Phosphofructokinase superfamily	SUPERFAMILY	SSF53784	Phosphofructokinase	coord: 355..536
IPR035966	Phosphofructokinase superfamily	SUPERFAMILY	SSF53784	Phosphofructokinase	coord: 28..369

Main menu

ATP-dependent 6-phosphofructokinase

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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