terminal uridylyltransferase 4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026950 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Match: TUT7 (terminal uridylyl transferase 7 [Source:HGNC Symbol;Acc:HGNC:25817]) HSP 1 Score: 230.335 bits (586), Expect = 3.036e-62 Identity = 137/426 (32.16%), Postives = 221/426 (51.88%), Query Frame = 1
Query: 2191 LQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
+ + D Y F + I + C LC +GH K+ C R + L + +L L +K P + E I + ++ +P + LFGS+ NGF K SDLDVC+ + L++ + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P +++DGW+ ++ I + W++ ++ LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 230 VDQSDFFYEFSKLIFTKGKSPTVVCSLCKREGHLKKDCPEDFKRIQ--LEPLPPLTPKFLNILDQVCIQCYKDFSPTIIEDQAREHIRQNLESFIRQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTI---NGLETAEGLDCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKKPEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDYPSKMEY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 649 HSP 2 Score: 78.9518 bits (193), Expect = 4.568e-14 Identity = 46/183 (25.14%), Postives = 91/183 (49.73%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ + P++P L ++ + + + + V+ + + + + + W+ N S+ +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 402 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKK-------PEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 577
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Match: TUT7 (terminal uridylyl transferase 7 [Source:HGNC Symbol;Acc:HGNC:25817]) HSP 1 Score: 230.72 bits (587), Expect = 1.673e-60 Identity = 137/426 (32.16%), Postives = 221/426 (51.88%), Query Frame = 1
Query: 2191 LQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
+ + D Y F + I + C LC +GH K+ C R + L + +L L +K P + E I + ++ +P + LFGS+ NGF K SDLDVC+ + L++ + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P +++DGW+ ++ I + W++ ++ LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 941 VDQSDFFYEFSKLIFTKGKSPTVVCSLCKREGHLKKDCPEDFKRIQ--LEPLPPLTPKFLNILDQVCIQCYKDFSPTIIEDQAREHIRQNLESFIRQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTI---NGLETAEGLDCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKKPEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDYPSKMEY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 1360 HSP 2 Score: 113.235 bits (282), Expect = 2.149e-24 Identity = 119/456 (26.10%), Postives = 213/456 (46.71%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQ-LKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP----------NLHKLAELQSQELS-DVSV---SRVLVNDTQLSYVNG----------MQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA-FKKVNFVEITLSNLKPIANVTP-IFTKDN 1512
E L I L + +P AK+ C++C ++ I A KH ++ HK+NI + + LLT LP PT Q+ AV ++ ++E + NE +R+ + M + + P L + SDVN+ ++ P + + L +++ KN + V+ F + C++K G+ +SA N L ++ + K++ + L FRY+A ID+ ++G P F+++ IF+LQQ P+LP +L KL Q++ DV + + DT ++ + I + + P+ + + +LW+ LL+FY ++FN ++SIR E V + + +R+AIEDP+ +KRN+ NS V +++ L+ + YFA+P T + K +N + + K + N P + TKD+
Sbjct: 222 ERLKRDCIDRLKRRPRNYPTAKYTCRLCDVLIESIAFAHKHIKEKRHKKNIKEKQEEELLTTLPPPTPSQINAVGIAIDKVVQE-FGLHNENLEQRLEIKRIMENVFQHKLPDCSLRL-YGSSCSRLGFKNSDVNIDIQF-PAIMSQPDVLLLVQE---CLKNSDSFIDVDADFHARVPVVVCREKQSGLLCKVSAGNENACLTTKHLTALGKLEPKLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAIFFLQQRKEPLLPVYLGSWIEGFSLSKLGNFNLQDIEKDVVIWEHTDSAAGDTGITKEEAPRETPIKRGQVSLILDVKHQPS--VPVGQLWVELLRFYALEFNLADLVISIRVKELVSRELKDWPKKRIAIEDPYSVKRNVARTLNSQPVFEYILHCLRTTYKYFALPHKITKSSLLKPLNAITCISEHSKEVINHHPDVQTKDD 669 HSP 3 Score: 79.337 bits (194), Expect = 4.961e-14 Identity = 74/299 (24.75%), Postives = 133/299 (44.48%), Query Frame = 1
Query: 2500 QLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI-LQELYEG--------------ERPTLYIDGWD-AWYQTNITI-------------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECFAHILK 3300
+L+GS+ + K SD+++ ++F +S +V L + LK ++ +D A+VP++ K + + +S N TK L L P + L +Y AK C I +G L Y + LM I FLQ+R +LP+ L EG E+ + + D A T IT ++ + + K ++ + LW++ +YA+EF++ V++IR + + + K+W +A+EDP+++ N+ +L+ + YIL C K
Sbjct: 337 RLYGSSCSRLGFKNSDVNIDIQFPA-----IMSQPDV---LLLVQECLKNSDSFIDVDADFHARVPVVVCREKQSGLLCKVSAGNENACLTTKHLTALGKLEPKLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAIFFLQQRKEPLLPVYLGSWIEGFSLSKLGNFNLQDIEKDVVIWEHTDSAAGDTGITKEEAPRETPIKRGQVSLILDVKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELVSRELKDWPKKRIAIEDPYSVKRNVARTLNSQPVFEYILHCLRTTYK 627 HSP 4 Score: 78.5666 bits (192), Expect = 9.918e-14 Identity = 46/183 (25.14%), Postives = 91/183 (49.73%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ + P++P L ++ + + + + V+ + + + + + W+ N S+ +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 1113 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKK-------PEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 1288
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Match: TUT4 (terminal uridylyl transferase 4 [Source:HGNC Symbol;Acc:HGNC:28981]) HSP 1 Score: 228.794 bits (582), Expect = 9.869e-60 Identity = 149/457 (32.60%), Postives = 233/457 (50.98%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNA--------KFKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + +SD D+ +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 874 CNCKATEDASDLNDD------DNLPTQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1308 HSP 2 Score: 139.813 bits (351), Expect = 1.882e-32 Identity = 125/483 (25.88%), Postives = 224/483 (46.38%), Query Frame = 1
Query: 166 SAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQELSDVSVSRVLVNDTQLSYVNGM-QWIKQLWNPPANE-----------------------------MSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA----FKKVNFVEITLSNLKPI 1479
+ VI++ E L E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ KN L + VE F + C+D K G+ +SA N + L ++L+ KI+ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + + + N + + N + + K + P ++ +SL +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G FKK +I SN KP+
Sbjct: 261 ATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIELAKE-HGITDDDLRVRQEIVEEMSKVITTFLPECSLRL-YGSSLTRFALKSSDVNIDIKF-PPKMNHPDLL--IKVLGILKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKI--SNKKPV 736 HSP 3 Score: 85.8853 bits (211), Expect = 4.940e-16 Identity = 51/183 (27.87%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K +L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1064 RSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1239 HSP 4 Score: 82.8037 bits (203), Expect = 5.255e-15 Identity = 77/339 (22.71%), Postives = 149/339 (43.95%), Query Frame = 1
Query: 2419 NDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+D ++ ++I +++++++ P +L+GS++ F K+SD+++ ++F N +K +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + +I NK + +SN +++ LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 370 DDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSSDVNIDIKF-------PPKMNHPDLLIKVLGI-LKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 700
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Match: TUT4 (terminal uridylyl transferase 4 [Source:HGNC Symbol;Acc:HGNC:28981]) HSP 1 Score: 228.409 bits (581), Expect = 1.112e-59 Identity = 149/457 (32.60%), Postives = 233/457 (50.98%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNA--------KFKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + +SD D+ +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 874 CNCKATEDASDLNDD------DNLPTQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1308 HSP 2 Score: 139.813 bits (351), Expect = 1.834e-32 Identity = 125/483 (25.88%), Postives = 224/483 (46.38%), Query Frame = 1
Query: 166 SAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQELSDVSVSRVLVNDTQLSYVNGM-QWIKQLWNPPANE-----------------------------MSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA----FKKVNFVEITLSNLKPI 1479
+ VI++ E L E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ KN L + VE F + C+D K G+ +SA N + L ++L+ KI+ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + + + N + + N + + K + P ++ +SL +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G FKK +I SN KP+
Sbjct: 261 ATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIELAKE-HGITDDDLRVRQEIVEEMSKVITTFLPECSLRL-YGSSLTRFALKSSDVNIDIKF-PPKMNHPDLL--IKVLGILKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKI--SNKKPV 736 HSP 3 Score: 85.8853 bits (211), Expect = 4.939e-16 Identity = 51/183 (27.87%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K +L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1064 RSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1239 HSP 4 Score: 82.8037 bits (203), Expect = 5.389e-15 Identity = 77/339 (22.71%), Postives = 149/339 (43.95%), Query Frame = 1
Query: 2419 NDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+D ++ ++I +++++++ P +L+GS++ F K+SD+++ ++F N +K +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + +I NK + +SN +++ LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 370 DDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSSDVNIDIKF-------PPKMNHPDLLIKVLGI-LKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 700
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Match: TUT4 (terminal uridylyl transferase 4 [Source:HGNC Symbol;Acc:HGNC:28981]) HSP 1 Score: 218.779 bits (556), Expect = 5.393e-57 Identity = 138/406 (33.99%), Postives = 218/406 (53.69%), Query Frame = 1
Query: 2125 NIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLR------AELNAK--FKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKL 3303
N K + +SD D+ +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + +L K F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL
Sbjct: 804 NCKATEDASDLNDD------DNLPTQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKL 1190 HSP 2 Score: 148.673 bits (374), Expect = 2.983e-35 Identity = 122/426 (28.64%), Postives = 205/426 (48.12%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA----NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA----FKKVNFVEITLSNLKPI 1479
E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ KN L + VE F + C+D K G+ +SA N + L ++L+ KI+ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L L S W+P A N +SL +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G FKK +I SN KP+
Sbjct: 282 ERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIELAKEH-GITDDDLRVRQEIVEEMSKVITTFLPECSLRL-YGSSLTRFALKSSDVNIDIKF-PPKMNHPDLL--IKVLGILKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCL-----LGS------------------------------WSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKI--SNKKPV 665 HSP 3 Score: 92.8189 bits (229), Expect = 4.117e-18 Identity = 67/290 (23.10%), Postives = 136/290 (46.90%), Query Frame = 1
Query: 2419 NDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+D ++ ++I +++++++ P +L+GS++ F K+SD+++ ++F N +K +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +P L +W + N+ +++ LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 370 DDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSSDVNIDIKF-------PPKMNHPDLLIKVLGI-LKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQR-------------KPPLLPCLLGSWSPLALETPNR---------VSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 629 HSP 4 Score: 86.2705 bits (212), Expect = 3.341e-16 Identity = 51/181 (28.18%), Postives = 93/181 (51.38%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K +L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 995 GLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1168
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Match: cid-1 (Caffeine Induced Death (S. pombe Cid) homolog [Source:UniProtKB/TrEMBL;Acc:Q09409]) HSP 1 Score: 156.762 bits (395), Expect = 3.648e-38 Identity = 114/331 (34.44%), Postives = 175/331 (52.87%), Query Frame = 1
Query: 2506 FGSTINGFMTKASDLDVCLEFYVDD-KLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNN---IQGDISINNSLGLQNTKMLKVYADLSPCVKL--LGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNK-KSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLH-SNSDNLNRPDNVHYMLLEPSVLRPPNGNM----RVCFSCNKQGHFARDCVDKG 3453
FGS + G SD+D+CL F D + L+ EV + +++ L+K + V+R+ I+ AKVPI+KF +K +N I DIS N L + NT +LK Y+ +P + L +K AK+CEIGDAS+G+LSSY +++MLI +LQ +LP LQE + + R +D WD + T + + W K K +C L + +F YY+ FD VV R+ L K++K W + VEDPF+LSHNL ++ + +I++ F + + S +NR D+ + +LR N R C C++ GHF C +
Sbjct: 1054 FGSVMTGLSVNCSDIDICLRFGDGDVPPKDLTAKEVIQKTESV---LRKCHLVKRVQAIVTAKVPIVKFQVKLSNGAIIDVDISYYNILAIYNTALLKEYSLWTPDKRFAKLALFVKTWAKNCEIGDASRGSLSSYCHVIMLISYLQNCDPPVLPRLQEDFRSDNRERRLVDNWDTSFAQVETSLLQRWPKNKESC----AQLLIGYFDYYS-RFDFRNFVVQCRREMILSKMEKEWPRP-LCVEDPFDLSHNLSSGVNKKMFVFIMKVFINSRAVFMSEKPPMNR-DHTFLSSYQHQLLRKCNQGSAPTDRQCHQCHRIGHFVESCPQRA 1374 HSP 2 Score: 62.3882 bits (150), Expect = 2.634e-9 Identity = 98/440 (22.27%), Postives = 183/440 (41.59%), Query Frame = 1
Query: 235 LSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTK-NLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSG-------------LFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFID---QIQDGLFPSEAFSILVIFYLQQLS-IPILPNL---------------HKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTE--PVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKKVNFV 1449
L+S I L FP+A + C+ C YH++ I A H + + H ++ + + +LL +P PT EA E +E L+ + + + R+ G+ + + + I ++ DV L P + Y ++ + + +F Q++ +I D + F ++ + + ++R+Q SEL++ Y+ + +L R +A KA +D + + GL P F I+ I +LQQ+ +P+L L K A+LQS+E + + + + +K+ WN LAEL+IG ++Y+ V I+ T+ P+ + + L + DPF N+L + F + L F+ F++P+ + GP +V+FV
Sbjct: 252 LTSCLIKKLVDRLEAFPEAIYYCEKCDYHINTIGHAKAHLESSTHFDDVKRQEQREHLLKIIPPPT----EAHLESVEKVLEGTL--KSYQQVRQNGIEIGETIIYYLSTTVFPSIGLQDVILRPFGSVTYDVTL-----PDSDYNVAYTMSIPEGTPIFALLEQVRKKIADDGHPADHSMEMGTPSTIIFTFKGVRVKLCWMSCFNHRIQLHFSELMKTYVNLREEVAQFLQLIRLWATKAGLDSKNKPRIGL-PRYGFDIMAIHFLQQIGCLPVLHELFEAGPETEKIEEMSAEKTAKLQSEEAGQRRMRLMSRYEKNTEKIGTKFDLKKSWN-------LAELFIGFFRYYVERHRD----VVIQITQLIPMSRDVSRWNKKILHVVDPFR-GDNVLSIPKVSTWQPFFFNCLLTSFVSFSIPRTKNGPLI-EVSFV 666 HSP 3 Score: 54.299 bits (129), Expect = 7.408e-7 Identity = 53/178 (29.78%), Postives = 78/178 (43.82%), Query Frame = 1
Query: 802 ISASNRLQHLNSELIRLY--LKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFY-LVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
IS N L N+ L++ Y D R LA + +A I G S I++I YLQ P+LP L + ++E R LV++ S+ + Q W P N+ S A+L IG +Y DF + ++V R E +L R L +EDPFD+ N+
Sbjct: 1133 ISYYNILAIYNTALLKEYSLWTPDKRFAKLALFVKTWAKNCEIGDASRGSLSSYCHVIMLISYLQNCDPPVLPRLQEDFRSDNRE-------RRLVDNWDTSFAQVETSLLQRW--PKNKESCAQLLIGYFDYYSRFDFRN--FVVQCR-REMILSKMEKEWPRPLCVEDPFDLSHNL 1298 HSP 4 Score: 50.8322 bits (120), Expect = 9.397e-6 Identity = 45/182 (24.73%), Postives = 77/182 (42.31%), Query Frame = 1
Query: 2749 NNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQG--TLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDA--------------------WYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKN---WQSAYMAVEDPF 3219
N+ + L ++++K Y +L V LI+ A + ++ L Y + +M IHFLQ+ G LP+L EL+E T I+ A Y+ N I ++ K + N+ L++ FF YY +++ + Q QL + ++ W + V DPF
Sbjct: 447 NHRIQLHFSELMKTYVNLREEVAQFLQLIRLWATKAGLDSKNKPRIGLPRYGFDIMAIHFLQQIGCLPVLHELFEAGPETEKIEEMSAEKTAKLQSEEAGQRRMRLMSRYEKNTEKIGTKFDLKKSW--NLAELFIGFFRYY-----VERHRDVVIQITQLIPMSRDVSRWNKKILHVVDPF 621
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Match: pup-2 (Poly(U) Polymerase [Source:UniProtKB/TrEMBL;Acc:Q09408]) HSP 1 Score: 137.502 bits (345), Expect = 1.517e-33 Identity = 86/274 (31.39%), Postives = 142/274 (51.82%), Query Frame = 1
Query: 2503 LFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSP-CVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG---ILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSN 3312
++GST NGF T+ D+D+ L F + +++ V ++ + + L Y+ AKVPI++F + +++ DIS N L L NT++L+ Y P + LG +K AK +GDAS+G+LSSYA+++MLIH+LQ+ +LP LQE+ + +Y+ G++ +Y + + C ++ L++ F YYA FD V+ + K FK D+ W M + DPF HNL + + YI C H K+ ++
Sbjct: 171 IYGSTRNGFGTRFCDVDMSLSFSPSPPSWATNSDRV---MRAVAKALVDFPKAVDERYV-NAKVPIVRFRSSDMDMEADISYKNDLALHNTQLLQQYCKWDPERLPTLGVWVKAWAKRSGVGDASKGSLSSYAWIVMLIHYLQQVEPIPVLPCLQEMNHQKSENVYVQGYNTYYWKFVDTA-----RTRRCRASVVDLFVGFLDYYATYFDYSTNVIQMVSKKLEFKPDR-WCKYPMCIADPFETDHNLAQGVDMPMFEYIRSCMEHSKKVFTD 434
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Match: gld-2 (Poly(A) RNA polymerase gld-2 [Source:UniProtKB/Swiss-Prot;Acc:O17087]) HSP 1 Score: 95.1301 bits (235), Expect = 1.797e-19 Identity = 75/250 (30.00%), Postives = 133/250 (53.20%), Query Frame = 1
Query: 2452 DKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIK--FYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGERPTLYIDGWDAWYQTNITI--------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQL 3165
D + + ++P++ + GS++NGF +SD+D+CL D Q N+ L I L+ V+ IL AKVPI++ F ++I D++ NNS+ ++NT +L Y+ V+ L +++K AK I DA++ + +SY+ +LM+IHFLQ +LP LQ+ Y P + + D N+T+ I++ ++K+ + L + F YYA EF+ D++ ++IRQ +++
Sbjct: 216 DMLYTAISPVFPLSGLYVVGSSLNGFGNNSSDMDLCLMITNKDLDQK---NDAVVVLNLILSTLQYEKFVESQKLIL-AKVPILRINFAAPFDDITVDLNANNSVAIRNTHLLCYYSSYDWRVRPLVSVVKEWAKRKGINDANKSSFTSYSLVLMVIHFLQCGPTKVLPNLQQSY----PNRFSNKVDV-RTLNVTMALEEVADDIDQSLSEKT----TLGELLIGFLDYYANEFNYDRDAISIRQGRRV 452
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Match: gld-2 (Poly(A) RNA polymerase gld-2 [Source:UniProtKB/Swiss-Prot;Acc:O17087]) HSP 1 Score: 95.1301 bits (235), Expect = 2.051e-19 Identity = 75/250 (30.00%), Postives = 133/250 (53.20%), Query Frame = 1
Query: 2452 DKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIK--FYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGERPTLYIDGWDAWYQTNITI--------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQL 3165
D + + ++P++ + GS++NGF +SD+D+CL D Q N+ L I L+ V+ IL AKVPI++ F ++I D++ NNS+ ++NT +L Y+ V+ L +++K AK I DA++ + +SY+ +LM+IHFLQ +LP LQ+ Y P + + D N+T+ I++ ++K+ + L + F YYA EF+ D++ ++IRQ +++
Sbjct: 333 DMLYTAISPVFPLSGLYVVGSSLNGFGNNSSDMDLCLMITNKDLDQ---KNDAVVVLNLILSTLQYEKFVESQKLIL-AKVPILRINFAAPFDDITVDLNANNSVAIRNTHLLCYYSSYDWRVRPLVSVVKEWAKRKGINDANKSSFTSYSLVLMVIHFLQCGPTKVLPNLQQSY----PNRFSNKVDV-RTLNVTMALEEVADDIDQSLSEKT----TLGELLIGFLDYYANEFNYDRDAISIRQGRRV 569
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Match: gld-2 (Poly(A) RNA polymerase gld-2 [Source:UniProtKB/Swiss-Prot;Acc:O17087]) HSP 1 Score: 95.1301 bits (235), Expect = 2.241e-19 Identity = 75/250 (30.00%), Postives = 133/250 (53.20%), Query Frame = 1
Query: 2452 DKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIK--FYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGERPTLYIDGWDAWYQTNITI--------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQL 3165
D + + ++P++ + GS++NGF +SD+D+CL D Q N+ L I L+ V+ IL AKVPI++ F ++I D++ NNS+ ++NT +L Y+ V+ L +++K AK I DA++ + +SY+ +LM+IHFLQ +LP LQ+ Y P + + D N+T+ I++ ++K+ + L + F YYA EF+ D++ ++IRQ +++
Sbjct: 269 DMLYTAISPVFPLSGLYVVGSSLNGFGNNSSDMDLCLMITNKDLDQ---KNDAVVVLNLILSTLQYEKFVESQKLIL-AKVPILRINFAAPFDDITVDLNANNSVAIRNTHLLCYYSSYDWRVRPLVSVVKEWAKRKGINDANKSSFTSYSLVLMVIHFLQCGPTKVLPNLQQSY----PNRFSNKVDV-RTLNVTMALEEVADDIDQSLSEKT----TLGELLIGFLDYYANEFNYDRDAISIRQGRRV 505
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Match: MTPAP (gene:FBgn0024360 transcript:FBtr0332288) HSP 1 Score: 98.5969 bits (244), Expect = 1.436e-20 Identity = 78/307 (25.41%), Postives = 153/307 (49.84%), Query Frame = 1
Query: 2455 KITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEF------------YVDDKLQSLSNNEVSNRLKTIGRELKK--------LNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT-LSSYAYMLMLIHFLQ--KRGILPILQELYEGERPT---LYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNL----CGSLSLE--NARYILE 3279
++ + + ++P A+ Q FGS++NGF DLD+ L F V +L + +SN R ++ L V + IL+A+VPIIK++ ++ +++ D+S++N G +++L ++ ++ P V+ L I+ A++C + + S G +S+++ +++ FLQ ++ ILP + L + P + DG + + N +++L + N ++ L L FF +Y+ +FD +++ + K L K D + +E N+S N+ C L +E NA ++LE
Sbjct: 200 QVQQAIAGMFPAAQAQPFGSSVNGFGRMGCDLDLILRFDSDMGAKIPLEAAVPSRLVYHTKENLSNGRSQTQRHMECFGDMLHLFLPGVCHVRRILQARVPIIKYHHEHLDLEVDLSMSNLTGFYMSELLYMFGEMDPRVRPLTFTIRRWAQTCGLTNPSPGRWISNFSLTCLVMFFLQQLRQPILPTIGALAKAAEPGDSRVTEDGINCTFTRN---VDRLGFRSRN-QSSLSELLLQFFEFYS-QFDFHNRAISLNEGKPLSKPDHSAMYIVNPLEQLLNVSKNVSLEECERLRIEVRNAAWVLE 501
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Match: MTPAP (gene:FBgn0024360 transcript:FBtr0070236) HSP 1 Score: 98.5969 bits (244), Expect = 1.436e-20 Identity = 78/307 (25.41%), Postives = 153/307 (49.84%), Query Frame = 1
Query: 2455 KITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEF------------YVDDKLQSLSNNEVSNRLKTIGRELKK--------LNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT-LSSYAYMLMLIHFLQ--KRGILPILQELYEGERPT---LYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNL----CGSLSLE--NARYILE 3279
++ + + ++P A+ Q FGS++NGF DLD+ L F V +L + +SN R ++ L V + IL+A+VPIIK++ ++ +++ D+S++N G +++L ++ ++ P V+ L I+ A++C + + S G +S+++ +++ FLQ ++ ILP + L + P + DG + + N +++L + N ++ L L FF +Y+ +FD +++ + K L K D + +E N+S N+ C L +E NA ++LE
Sbjct: 200 QVQQAIAGMFPAAQAQPFGSSVNGFGRMGCDLDLILRFDSDMGAKIPLEAAVPSRLVYHTKENLSNGRSQTQRHMECFGDMLHLFLPGVCHVRRILQARVPIIKYHHEHLDLEVDLSMSNLTGFYMSELLYMFGEMDPRVRPLTFTIRRWAQTCGLTNPSPGRWISNFSLTCLVMFFLQQLRQPILPTIGALAKAAEPGDSRVTEDGINCTFTRN---VDRLGFRSRN-QSSLSELLLQFFEFYS-QFDFHNRAISLNEGKPLSKPDHSAMYIVNPLEQLLNVSKNVSLEECERLRIEVRNAAWVLE 501
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Match: Gld2 (gene:FBgn0038934 transcript:FBtr0305038) HSP 1 Score: 97.8265 bits (242), Expect = 4.875e-20 Identity = 71/243 (29.22%), Postives = 126/243 (51.85%), Query Frame = 1
Query: 2431 KIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINK---LWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIR 3150
KI +++ I + YP L GS+I+ F +K SD+D+C+ + + S E L + L + N Q + +++A+VPI++F + + ++ DI+ NNS+G++NT +L Y+ L V+ + +K A+ I +A T+SSY+ MLM+IHFLQ P+L L+ LY + + + ++ + +S+ + + L L F +YY+V FD K ++IR
Sbjct: 955 KIKMRLWRAIYTVAMKNYPRYGLYLVGSSISYFGSKCSDMDICMLACTNPNIDS--RMEAVYHLHVMKELLGRTNMFQDFN-LIEARVPILRFTDRCHKVEVDINFNNSVGIRNTHLLYCYSQLDWRVRPMALTVKQWAQYHNINNAKNMTISSYSLMLMVIHFLQVGASPPVLPCLHN-----LYPEKFGLLQPNDFGYVDMNEVMAPYQSDNSQTLGDLLLSFLHYYSV-FDYGKYAISIR 1188
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Match: Gld2 (gene:FBgn0038934 transcript:FBtr0310204) HSP 1 Score: 97.8265 bits (242), Expect = 4.875e-20 Identity = 71/243 (29.22%), Postives = 126/243 (51.85%), Query Frame = 1
Query: 2431 KIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINK---LWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIR 3150
KI +++ I + YP L GS+I+ F +K SD+D+C+ + + S E L + L + N Q + +++A+VPI++F + + ++ DI+ NNS+G++NT +L Y+ L V+ + +K A+ I +A T+SSY+ MLM+IHFLQ P+L L+ LY + + + ++ + +S+ + + L L F +YY+V FD K ++IR
Sbjct: 955 KIKMRLWRAIYTVAMKNYPRYGLYLVGSSISYFGSKCSDMDICMLACTNPNIDS--RMEAVYHLHVMKELLGRTNMFQDFN-LIEARVPILRFTDRCHKVEVDINFNNSVGIRNTHLLYCYSQLDWRVRPMALTVKQWAQYHNINNAKNMTISSYSLMLMVIHFLQVGASPPVLPCLHN-----LYPEKFGLLQPNDFGYVDMNEVMAPYQSDNSQTLGDLLLSFLHYYSV-FDYGKYAISIR 1188
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Match: wisp (gene:FBgn0260780 transcript:FBtr0342742) HSP 1 Score: 88.5817 bits (218), Expect = 3.244e-17 Identity = 86/315 (27.30%), Postives = 154/315 (48.89%), Query Frame = 1
Query: 2374 LRAELNAKFKSIVPNNDRPKIAEKIFDKI-TEIVKALYPIARPQLFGSTINGFMTKASDLDVCL-------EFYVDDKLQSLSNNEVSNR----LKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR---GILPILQELYE-----GERPTLYIDGWDAWYQTNITIINKLWNKKS-NCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSK-------QLFKIDKN--WQSAYMAVEDPFNLS 3228
L AE+ +F+ +++ K+ +++ + + + ++ R L GSTI GF T +SD+D+CL +NE L LK Q + +++A+VPI++F +N I+ D++ NN +G++NT +L++YA + + L ++K A+ +I DA + T+SSY+ +LM++H+LQ +LP L LY G++ L +D +I + ++ N E+L L FF YY+ FD ++IR ++ K KN +Q + +E+PF+LS
Sbjct: 970 LSAEIWKRFRGAQQTHNKFKLKMRLWRYLYLWMHQPMFERYRICLVGSTITGFGTDSSDIDMCLLPEQGVHPHQHQYHQHHHFHNEKRTEALIILTLFNAVLKDTEVFQDFN-LIEARVPILRFKDISNGIEVDLNFNNCVGIKNTYLLQLYAQMDWRTRPLVVIVKLWAQYHDINDAKRMTISSYSLVLMVLHYLQHACVPHVLPCLHSLYPEKFQLGQQDCLDLD-----------LIEPIEPYQALNTQTLGEHL-LGFFKYYST-FDFRNFAISIRTGGVLPVSTCRMAKSPKNDVYQWKELNIEEPFDLS 1270
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Match: CABZ01065131.1 (pep chromosome:GRCz11:23:42272588:42289293:1 gene:ENSDARG00000101404.2 transcript:ENSDART00000164907.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01065131.1) HSP 1 Score: 210.305 bits (534), Expect = 1.125e-56 Identity = 115/323 (35.60%), Postives = 182/323 (56.35%), Query Frame = 1
Query: 2503 LFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM----LLEPSVLRPPNGNMRVCFSCNKQGHFARDCVDK 3450
LFGS+ NGF + SDLD+C+ D + L+ E+ ++ + + LKK ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G P +DGW+A++ ++ + + + + LWL +Y EFD + V++IRQ K+L +K W + DPF+L+HNL +S + +I++ F + KL Y +L L P N R C C K GH+ +DC +
Sbjct: 7 LFGSSKNGFGFRDSDLDICMTLEGHDTAEKLNCKEI---IEGLAKVLKKHTGLRNILPITTAKVPIVKFEHRQSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVLQEIFDGNTTPQRMVDGWNAFFFDDLDELRRRLPELHQNRETVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTYFCFLLSDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPQPGTEADYFFDSKVLTDGELAP---NDRCCRICGKIGHYMKDCPKR 323 HSP 2 Score: 70.0922 bits (170), Expect = 1.650e-11 Identity = 44/180 (24.44%), Postives = 87/180 (48.33%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + + R++ + + + ++L N ++ ELW+GLL+FY +F+ +++SIR + + ++ + DPFD+ N+
Sbjct: 78 GLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVLQEIFD------GNTTPQRMVDGWNAFFFDDLDELRRRLPELHQNRETVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTYFCFLLSDPFDLNHNL 251
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Match: tent2 (terminal nucleotidyltransferase 2 [Source:NCBI gene;Acc:553626]) HSP 1 Score: 125.946 bits (315), Expect = 1.201e-29 Identity = 82/255 (32.16%), Postives = 148/255 (58.04%), Query Frame = 1
Query: 2470 VKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQK--RGILPILQELYEGERPTLYIDGWD-AWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKID-KNWQSAYMAVEDPFN 3222
++ ++P A+ L GS++NGF +++SD D+CL V ++ + L + + L KL+ +++ +++AKVPI+KF + + ++ D++ NN++G++NT +L+ YA + V+ L +IK A I DAS+GTLSSY +LM++H+LQ ++P LQ Y PT + D + + I ++ + ++ L+L F YYA F DK+V+++R ++ L K + K W+ ++ VE+PFN
Sbjct: 194 IQKIFPCAKVFLGGSSLNGFGSRSSDADLCL---VIEEGPVNHRKDAVYVLSLVRKLLYKLSYIEK-PQLIRAKVPIVKFRDRISGVEFDLNFNNTVGIRNTFLLRTYAFVEKRVRPLVLVIKKWANHHCINDASRGTLSSYTLVLMVLHYLQTLPEPVIPCLQRDY----PTCFDPKMDIHLVPSGPSDIPAFVSRNQS---SLGDLFLGFLRYYATVFKWDKQVISVRMARTLPKSNCKEWKDKFICVEEPFN 437
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Match: CABZ01032476.1 (terminal uridylyl transferase 7 [Source:NCBI gene;Acc:562358]) HSP 1 Score: 122.865 bits (307), Expect = 1.106e-27 Identity = 104/390 (26.67%), Postives = 188/390 (48.21%), Query Frame = 1
Query: 292 KFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETG-YLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK-----LAELQSQELSDVSVSRVL--VNDTQLSYVNGMQWIKQLWNPPA----NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
KF C C +DI A+ H ++ H++ +L LL +P P K +V+ LE+ + E + +E+ +R + + ++ + P + + SDVN+ ++ P++ + L+A E S K+ L VE F + + CK+K G+ +SA N L + + ++ + L FRY+A +DQ+++G PS F+++VI +LQ+ PILP + L++L+S L+ V VL + T S ++ + +K+ P A + + L +LWI LL++Y ++F ++S+R + + + +R+AIEDPF ++RN+ NS + D+L L+ + YFA P T
Sbjct: 139 KFRCDKCKVFCEDISPALSHIRERSHRKKAKELQKLALLLSIPPPAKPHCLSVSSALESIVTE-FGLNDEDLKQRQNILALIEKVLHPVLPECQFRL-YGSSSTKFGFKDSDVNIDVKF-PSHFQHPDILLAAQEHLS---KSSLFDSVEGDFHRRMPVVVCKEKASGLICKVSAGNESACLTTAYLSEMADLEPQLVPLVICFRYWAKICCVDQMEEGGLPSYCFALMVISFLQRRKEPILPTYLESMGFPLSKLKSFSLTGVEKGHVLWVYDQTNDSSLDKDKAVKKGKAPLAFKSRSPVLLGKLWIELLRYYALEFQIPEKIISVRTNGELWRDLKDWPKKRIAIEDPFTVQRNVARTLNSQTMFDYLLHCLKTTYKYFASPSKSTA 522 HSP 2 Score: 93.2041 bits (230), Expect = 1.311e-18 Identity = 78/317 (24.61%), Postives = 148/317 (46.69%), Query Frame = 1
Query: 2416 NNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYE---GERPTLYIDGWDAWY------QTNITIINKLWNK-----------KSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILK 3300
N++ K + I I +++ + P + +L+GS+ F K SD+++ ++F S+ + + L L K + ++ ++P++ K + + +S N T L ADL P + L +Y AK C + +G L SY + LM+I FLQ+R ILP E + + + G + + QTN + ++K +K KS + + LW++ YYA+EF I ++++++R + +L++ K+W +A+EDPF + N+ +L N++ + + H LK
Sbjct: 205 NDEDLKQRQNILALIEKVLHPVLPECQFRLYGSSSTKFGFKDSDVNIDVKFP--------SHFQHPDILLAAQEHLSKSSLFDSVEGDFHRRMPVVVCKEKASGLICKVSAGNESACLTTAYLSEMADLEPQLVPLVICFRYWAKICCVDQMEEGGLPSYCFALMVISFLQRRKEPILPTYLESMGFPLSKLKSFSLTGVEKGHVLWVYDQTNDSSLDK--DKAVKKGKAPLAFKSRSPVLLGKLWIELLRYYALEFQIPEKIISVRTNGELWRDLKDWPKKRIAIEDPFTVQRNVARTL---NSQTMFDYLLHCLK 508 HSP 3 Score: 82.8037 bits (203), Expect = 2.540e-15 Identity = 53/184 (28.80%), Postives = 90/184 (48.91%), Query Frame = 1
Query: 2212 YLFD-RISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSL 2760
Y+F+ R + V CG+C + GH K+ C R + + L + + L ++ P++ K+ E I ++ P A+ LFGS+ NGF K SDLD+C+ D L + + ++++ + L+K + ++ I I AKVPI+KFY ++GDIS+ N+L
Sbjct: 660 YVFNKRFFTDGKSPVQICGVCKSDGHLKQDCPEDFQRVE--LERLPPMTRDFQKILSDVCEQCYRDFAPDDVELKVREHILQDFESFLRCQVPGAKLVLFGSSKNGFGFKQSDLDICMTLEGHDTAMGLDSMAI---IESLAKALRKHHGLRNILPITTAKVPIVKFYHTKTGLEGDISLYNTL 838
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Match: tut1 (terminal uridylyl transferase 1, U6 snRNA-specific [Source:NCBI gene;Acc:564388]) HSP 1 Score: 82.8037 bits (203), Expect = 2.057e-15 Identity = 89/370 (24.05%), Postives = 161/370 (43.51%), Query Frame = 1
Query: 2326 ECQDILESID-----LAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQS---------------------------LSNNEVSNRLKTIGRELKK-LNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT---LSSYAYMLMLIHFLQKRG--ILPILQEL--YEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKID-----------------KNWQSAY-----MAVEDPFNLSHNLCGSL 3249
+C++ L S+D L + + ++ +S+ ++ K+ + + + E+ +P + FGS++N F + DLD+ L+ Q+ LS + L+ + L+K + V ++ + A++P++KF K N+QGDI+INN L ++NTK L++ + + ++ L I+ AK ++ G L++YA L++I FLQ R +LP + +L E I+ WD + + + N + C L FF +Y+ +FD VV++R L D K +S+ M V DPF L+HN+ G+L
Sbjct: 138 DCKNTLISLDKLNFELCKAMSVNEQIQKVVESLELKDNEKKVRDLLVQLLQEVFTEFFPDCQIVPFGSSVNTFGLHSCDLDLFLDLENTKVFQARAKSSEQTGENQSEDCRSEDSILSDIDLSTASPAEILELVAVILRKCVPGVHKVQALSTARLPVVKFSHKELNLQGDITINNRLAVRNTKFLQLCSGIDSRLRPLVYTIRLWAKQKQLAGNLSGPGPLLNNYALTLLVIFFLQNRDPPVLPSVNQLKNMACEEEECAIEEWDCTFPSQPFSVPPSKNTEDLCT-----LLFGFFTFYS-KFDFPASVVSLRDGHALPITDFLKSDMEALKTADASSPKPKRSSAPRLGPMNVLDPFELNHNVAGNL 501
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Match: mtpap (mitochondrial poly(A) polymerase [Source:ZFIN;Acc:ZDB-GENE-101208-3]) HSP 1 Score: 79.337 bits (194), Expect = 2.150e-14 Identity = 70/287 (24.39%), Postives = 139/287 (48.43%), Query Frame = 1
Query: 2458 ITEIVKALYP--IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNR-------------------LKTIGRELKKLN-TVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT-LSSYAYMLMLIHFLQKR--GILPILQELYEGERPT--LYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNL 3237
+ +I A +P I RP FGST+N F D+D+ L+ D + + S + S L +G+ + + + IL+A+ PI++F + + Q D++ NN + ++++++L +Y L P V+ L ++ A++ I + G +++++ +M++ FLQ+R +LP L L E P+ I+G D ++++ I N + +E L +FF +Y F +K + IR+ K+ K + +A + +++PF + N+
Sbjct: 230 LGDIAGAYFPECIIRP--FGSTVNSFGKLGCDVDMILDL---DGIYARSQKKGSGLSLEYQVKTGPSERAVTQSILSVVGKCVDQFGPGCVGVQNILQARCPIVRFAHQPSGFQCDLTANNKVAMKSSELLFLYGHLDPRVRHLVFSVRCWARAHSITSSIPGAWITNFSLTVMVVFFLQQRSPAMLPTLDRLKELAGPSDKCVIEGNDCTIVSDLSKIALQKNTDT-----LEKLLQEFFEFYG-NFPFNKASINIRKGKEQSKPE----AAALHIQNPFEATLNV 501
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Match: tyms (thymidylate synthetase [Source:Xenbase;Acc:XB-GENE-1001385]) HSP 1 Score: 249.21 bits (635), Expect = 2.050e-66 Identity = 152/435 (34.94%), Postives = 235/435 (54.02%), Query Frame = 1
Query: 2188 NLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWL----VKLRAELNAKFK----SIVPNNDRPKIAEKIFDKITEIVKALYP-IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS----DNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+L ++DL+Y+FD+ I + C +C GH K D+C + + IDL L V+ R L+ K + PN+ + E+I + ++ + +R LFGS+ NGF + SDLD+C+ + + L+ E+ + L + LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA + P VK LG +K+ AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G E P +DGW+A++ N + + ++ LWL F +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL L R +L L P N R C C K GH+ +DC
Sbjct: 601 SLSEEDLLYIFDKDILTSGKPPTIVCSICKRDGHAK----------DDCPEDFKKIDLKPLPPMTVRFREILDRVCKRCYDELSPNSSEQQNREQILAYLERFIRKEFNNHSRLCLFGSSKNGFGFRDSDLDICMTLEGHENAKKLNCKEIIDGLAKV---LKKHPGLKNILAITTAKVPIVKFEHKESGVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPIYPIPGLEREYFFDSKVLTDGELAP---NDRCCRVCGKIGHYMKDC 1019 HSP 2 Score: 130.954 bits (328), Expect = 7.078e-30 Identity = 139/542 (25.65%), Postives = 245/542 (45.20%), Query Frame = 1
Query: 220 SLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK--LAELQSQELSDVSVSRVLVNDTQL---------------SYVNGMQWIKQ------------------------LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLR-----TGPAFKKVNFVEITLSNLK-PIANVTPIFTKDN--FTETINIIETDFKKFYTEQKSFYDVSKWPVELFVKACLLKILNSVFNSEIDIKNLVCLLEL 1695
+L + +S + L + S ++ +++CK+C H+++I A KH ++ HK+NI + +N L LP PT Q+ A+ L + KE + E++ R + M ++ + P L + + SD+N+ ++ + + Y+ ++ ++ +VE F +FCKD K G+T +SA N L +E + K++ L FRY+A ID +G PS F+++V+F+LQ+ PILP + +S++L D + V +D L S +G +Q +++ P E SL LW+ LLKFY ++F +++S+R + + +++ RRLAIEDPF +KRN+ NS V +++ ++ + + YFA PQ + T A K ++ E +N P A DN ++ETD K + Q+ F + V+ A +K+L S NS + K+ + + EL
Sbjct: 37 ALTQMFTSPHPLHLFQRSPEYSNCQYLCKLCTVHIENIQGAHKHIKEKRHKKNILEKQEENELRALPPPTPAQISALGLALLDAAKE-NGVSQEDFLLRQEIVKSMEKIIQMKLPECSLRM-YGSSLTRFAFTSSDINIDVKFPRKVLIKLFYVLSIACYT---------EVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGV-SDDCYLDWEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKE-SLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERFRAAYRYFACPQCKEQNKSTTDARKTLSKPEDNEANGSLPDAGTCGTDGADNESIPSAEEVLETD-GKLHPFQEEFSKLDLLSVDESHHA--VKLLRSEENSNCNGKDYLAIKEL 562 HSP 3 Score: 85.8853 bits (211), Expect = 5.027e-16 Identity = 51/180 (28.33%), Postives = 88/180 (48.89%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
GV IS N L N+ ++ Y ID R + L ++FA + I G S A+ ++V+++LQQ + P++P L ++ + Q V D N + + N S+ ELW+G L+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 777 GVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGK------NRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 950 HSP 4 Score: 82.0333 bits (201), Expect = 6.861e-15 Identity = 75/334 (22.46%), Postives = 146/334 (43.71%), Query Frame = 1
Query: 2434 IAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPI-------------LQELY-EGERPTLYIDGWDAWYQTN--------------------ITIINKLWNKKSN-------------CNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ ++I + +I++ P +++GS++ F +SD+++ ++F +V +L + + ++ AKVP++ K + + +S N T+ L+ L P + L +Y A+ C I ++G + SY + LM++ FLQKR ILP L + + +G Y+D W+ +N T +++L ++K+ ++ +LWL+ +Y +EF +++ V+++R L + +KNW +A+EDPF L N+ SL+ + YILE F
Sbjct: 133 LRQEIVKSMEKIIQMKLPECSLRMYGSSLTRFAFTSSDINIDVKF----------PRKVLIKLFYV----LSIACYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGVSDDCYLD-WEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKESLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERF 451
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Match: tyms (thymidylate synthetase [Source:Xenbase;Acc:XB-GENE-1001385]) HSP 1 Score: 249.21 bits (635), Expect = 2.064e-66 Identity = 152/435 (34.94%), Postives = 235/435 (54.02%), Query Frame = 1
Query: 2188 NLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWL----VKLRAELNAKFK----SIVPNNDRPKIAEKIFDKITEIVKALYP-IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS----DNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+L ++DL+Y+FD+ I + C +C GH K D+C + + IDL L V+ R L+ K + PN+ + E+I + ++ + +R LFGS+ NGF + SDLD+C+ + + L+ E+ + L + LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA + P VK LG +K+ AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G E P +DGW+A++ N + + ++ LWL F +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL L R +L L P N R C C K GH+ +DC
Sbjct: 622 SLSEEDLLYIFDKDILTSGKPPTIVCSICKRDGHAK----------DDCPEDFKKIDLKPLPPMTVRFREILDRVCKRCYDELSPNSSEQQNREQILAYLERFIRKEFNNHSRLCLFGSSKNGFGFRDSDLDICMTLEGHENAKKLNCKEIIDGLAKV---LKKHPGLKNILAITTAKVPIVKFEHKESGVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPIYPIPGLEREYFFDSKVLTDGELAP---NDRCCRVCGKIGHYMKDC 1040 HSP 2 Score: 128.642 bits (322), Expect = 4.282e-29 Identity = 114/441 (25.85%), Postives = 205/441 (46.49%), Query Frame = 1
Query: 220 SLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK--LAELQSQELSDVSVSRVLVNDTQL---------------SYVNGMQWIKQ------------------------LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLR 1416
+L + +S + L + S ++ +++CK+C H+++I A KH ++ HK+NI + +N L LP PT Q+ A+ L + KE + E++ R + M ++ + P L + + SD+N+ ++ + + Y+ ++ ++ +VE F +FCKD K G+T +SA N L +E + K++ L FRY+A ID +G PS F+++V+F+LQ+ PILP + +S++L D + V +D L S +G +Q +++ P E SL LW+ LLKFY ++F +++S+R + + +++ RRLAIEDPF +KRN+ NS V +++ ++ + + YFA PQ +
Sbjct: 38 ALTQMFTSPHPLHLFQRSPEYSNCQYLCKLCTVHIENIQGAHKHIKEKRHKKNILEKQEENELRALPPPTPAQISALGLALLDAAKE-NGVSQEDFLLRQEIVKSMEKIIQMKLPECSLRM-YGSSLTRFAFTSSDINIDVKFPRKVLIKLFYVLSIACYT---------EVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGV-SDDCYLDWEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKE-SLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERFRAAYRYFACPQCK 465 HSP 3 Score: 85.8853 bits (211), Expect = 4.720e-16 Identity = 50/180 (27.78%), Postives = 92/180 (51.11%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
GV IS N L N+ ++ Y ID R + L ++FA + I G S A+ ++V+++LQQ + P++P L ++ + Q + R++ + N + + + N S+ ELW+G L+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 798 GVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQE------TPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 971 HSP 4 Score: 82.0333 bits (201), Expect = 7.822e-15 Identity = 75/334 (22.46%), Postives = 146/334 (43.71%), Query Frame = 1
Query: 2434 IAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPI-------------LQELY-EGERPTLYIDGWDAWYQTN--------------------ITIINKLWNKKSN-------------CNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ ++I + +I++ P +++GS++ F +SD+++ ++F +V +L + + ++ AKVP++ K + + +S N T+ L+ L P + L +Y A+ C I ++G + SY + LM++ FLQKR ILP L + + +G Y+D W+ +N T +++L ++K+ ++ +LWL+ +Y +EF +++ V+++R L + +KNW +A+EDPF L N+ SL+ + YILE F
Sbjct: 134 LRQEIVKSMEKIIQMKLPECSLRMYGSSLTRFAFTSSDINIDVKF----------PRKVLIKLFYV----LSIACYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGVSDDCYLD-WEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKESLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERF 452
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Match: tyms (thymidylate synthetase [Source:Xenbase;Acc:XB-GENE-1001385]) HSP 1 Score: 249.21 bits (635), Expect = 2.967e-66 Identity = 152/435 (34.94%), Postives = 235/435 (54.02%), Query Frame = 1
Query: 2188 NLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWL----VKLRAELNAKFK----SIVPNNDRPKIAEKIFDKITEIVKALYP-IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS----DNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+L ++DL+Y+FD+ I + C +C GH K D+C + + IDL L V+ R L+ K + PN+ + E+I + ++ + +R LFGS+ NGF + SDLD+C+ + + L+ E+ + L + LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA + P VK LG +K+ AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G E P +DGW+A++ N + + ++ LWL F +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL L R +L L P N R C C K GH+ +DC
Sbjct: 803 SLSEEDLLYIFDKDILTSGKPPTIVCSICKRDGHAK----------DDCPEDFKKIDLKPLPPMTVRFREILDRVCKRCYDELSPNSSEQQNREQILAYLERFIRKEFNNHSRLCLFGSSKNGFGFRDSDLDICMTLEGHENAKKLNCKEIIDGLAKV---LKKHPGLKNILAITTAKVPIVKFEHKESGVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPIYPIPGLEREYFFDSKVLTDGELAP---NDRCCRVCGKIGHYMKDC 1221 HSP 2 Score: 134.806 bits (338), Expect = 5.380e-31 Identity = 148/556 (26.62%), Postives = 245/556 (44.06%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK--LAELQSQELSDVSVSRVLVNDTQL---------------SYVNGMQWIKQ------------------------LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLR-----TGPAFKKVNFVEITLSNLK-PIANVTPIFTKDN--FTETINIIETDFK-------KFYTEQKSFYDVSKW-PVELFVKACLL---------KILNSVFNSEIDIKNLVCLLEL 1695
E L YI L K S ++ +++CK+C H+++I A KH ++ HK+NI + +N L LP PT Q+ A+ L + KE + E++ R + M ++ + P L + + SD+N+ ++ P + + L ++ ++ +VE F +FCKD K G+T +SA N L +E + K++ L FRY+A ID +G PS F+++V+F+LQ+ PILP + +S++L D + V +D L S +G +Q +++ P E SL LW+ LLKFY ++F +++S+R + + +++ RRLAIEDPF +KRN+ NS V +++ ++ + + YFA PQ + T A K ++ E +N P A DN ++ETD K T Q D+ K P+ F K LL K+L S NS + K+ + + EL
Sbjct: 216 ERLERDYIYRLEKRSPEYSNCQYLCKLCTVHIENIQGAHKHIKEKRHKKNILEKQEENELRALPPPTPAQISALGLALLDAAKE-NGVSQEDFLLRQEIVKSMEKIIQMKLPECSLRM-YGSSLTRFAFTSSDINIDVKF-PRKMNQPDVL--IQVLDILKNCACYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGV-SDDCYLDWEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKE-SLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERFRAAYRYFACPQCKEQNKSTTDARKTLSKPEDNEANGSLPDAGTCGTDGADNESIPSAEEVLETDGKLHPFQEGDVSTIQYLGGDIDKTDPLTEFSKLDLLSVDESHHAVKLLRSEENSNCNGKDYLAIKEL 764 HSP 3 Score: 85.8853 bits (211), Expect = 5.006e-16 Identity = 50/180 (27.78%), Postives = 92/180 (51.11%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
GV IS N L N+ ++ Y ID R + L ++FA + I G S A+ ++V+++LQQ + P++P L ++ + Q + R++ + N + + + N S+ ELW+G L+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 979 GVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQE------TPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1152 HSP 4 Score: 83.9593 bits (206), Expect = 1.934e-15 Identity = 77/334 (23.05%), Postives = 147/334 (44.01%), Query Frame = 1
Query: 2434 IAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI-------------LQELY-EGERPTLYIDGWDAWYQTN--------------------ITIINKLWNKKSNCNINIEYL-------------WLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ ++I + +I++ P +++GS++ F +SD+++ ++F + ++ +V ++ I LK ++ AKVP++ K + + +S N T+ L+ L P + L +Y A+ C I ++G + SY + LM++ FLQKR ILP L + + +G Y+D W+ +N T +++L ++K+ L WL+ +Y +EF +++ V+++R L + +KNW +A+EDPF L N+ SL+ + YILE F
Sbjct: 309 LRQEIVKSMEKIIQMKLPECSLRMYGSSLTRFAFTSSDINIDVKF-----PRKMNQPDVLIQVLDI---LKNCACYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGVSDDCYLD-WEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKESLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERF 633
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Match: tyms (thymidylate synthetase [Source:Xenbase;Acc:XB-GENE-1001385]) HSP 1 Score: 240.736 bits (613), Expect = 1.005e-63 Identity = 147/426 (34.51%), Postives = 227/426 (53.29%), Query Frame = 1
Query: 2212 YLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWL----VKLRAELNAKFK----SIVPNNDRPKIAEKIFDKITEIVKALYP-IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS----DNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+L + S ++ C +C GH K D+C + + IDL L V+ R L+ K + PN+ + E+I + ++ + +R LFGS+ NGF + SDLD+C+ + + L+ E+ + L + LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA + P VK LG +K+ AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G E P +DGW+A++ N + + ++ LWL F +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL L R +L L P N R C C K GH+ +DC
Sbjct: 614 FLLIQFSPFSKPPTIVCSICKRDGHAK----------DDCPEDFKKIDLKPLPPMTVRFREILDRVCKRCYDELSPNSSEQQNREQILAYLERFIRKEFNNHSRLCLFGSSKNGFGFRDSDLDICMTLEGHENAKKLNCKEIIDGLAKV---LKKHPGLKNILAITTAKVPIVKFEHKESGVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPIYPIPGLEREYFFDSKVLTDGELAP---NDRCCRVCGKIGHYMKDC 1023 HSP 2 Score: 131.724 bits (330), Expect = 4.998e-30 Identity = 148/561 (26.38%), Postives = 247/561 (44.03%), Query Frame = 1
Query: 271 SNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK--LAELQSQELSDVSVSRVLVNDTQL---------------SYVNGMQWIKQ------------------------LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLR-----TGPAFKKVNFVEITLSNLK-PIANVTPIFTKDN--FTETINIIETDFK-------KFYTEQKSFYDVSKW-PVELFVKACLL---------KILNSVFNSEIDIKNLVCLLELK-TKGVAYFLIENYVKFKE 1749
S ++ +++CK+C H+++I A KH ++ HK+NI + +N L LP PT Q+ A+ L + KE + E++ R + M ++ + P L + + SD+N+ ++ P + + L + + KN +VE F +FCKD K G+T +SA N L +E + K++ L FRY+A ID +G PS F+++V+F+LQ+ PILP + +S++L D + V +D L S +G +Q +++ P E SL LW+ LLKFY ++F +++S+R + + +++ RRLAIEDPF +KRN+ NS V +++ ++ + + YFA PQ + T A K ++ E +N P A DN ++ETD K T Q D+ K P+ F K LL K+L S NS + K+ + + EL T +FL+ + F +
Sbjct: 72 SPEYSNCQYLCKLCTVHIENIQGAHKHIKEKRHKKNILEKQEENELRALPPPTPAQISALGLALLDAAKE-NGVSQEDFLLRQEIVKSMEKIIQMKLPECSLRM-YGSSLTRFAFTSSDINIDVKF-PRKMNQPDVLIQVLD---ILKNSCYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGV-SDDCYLDWEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKE-SLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERFRAAYRYFACPQCKEQNKSTTDARKTLSKPEDNEANGSLPDAGTCGTDGADNESIPSAEEVLETDGKLHPFQEGDVSTIQYLGGDIDKTDPLTEFSKLDLLSVDESHHAVKLLRSEENSNCNGKDYLAIKELTDTTEERHFLLIQFSPFSK 624 HSP 3 Score: 85.8853 bits (211), Expect = 4.964e-16 Identity = 50/180 (27.78%), Postives = 92/180 (51.11%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
GV IS N L N+ ++ Y ID R + L ++FA + I G S A+ ++V+++LQQ + P++P L ++ + Q + R++ + N + + + N S+ ELW+G L+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 781 GVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQE------TPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 954 HSP 4 Score: 76.6406 bits (187), Expect = 2.749e-13 Identity = 74/334 (22.16%), Postives = 144/334 (43.11%), Query Frame = 1
Query: 2434 IAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPI-------------LQELY-EGERPTLYIDGWDAWYQTN--------------------ITIINKLWNKKSNCNINIEYLWLD-------------FFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ ++I + +I++ P +++GS++ F +SD+++ ++F N+ ++ + ++ K + ++ AKVP++ K + + +S N T+ L+ L P + L +Y A+ C I ++G + SY + LM++ FLQKR ILP L + + +G Y+D W+ +N T +++L ++K+ L D +Y +EF +++ V+++R L + +KNW +A+EDPF L N+ SL+ + YILE F
Sbjct: 151 LRQEIVKSMEKIIQMKLPECSLRMYGSSLTRFAFTSSDINIDVKF-------PRKMNQPDVLIQVL--DILKNSCYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGVSDDCYLD-WEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKESLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERF 474
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Match: tyms (thymidylate synthetase [Source:Xenbase;Acc:XB-GENE-1001385]) HSP 1 Score: 239.58 bits (610), Expect = 2.105e-63 Identity = 162/504 (32.14%), Postives = 248/504 (49.21%), Query Frame = 1
Query: 2188 NLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWL----VKLRAELNAKFK----SIVPNNDRPKIAEKIFDKITEIVKALYP-IARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS----DNLNRPDNVHYMLLEPSVLRPPNGNMRV----------------------------------CFSCNKQGHFARDCVDKGNKRTPTKA--APNQQPGL----PSITAPLQF 3528
+L ++DL+Y+FD+ I + C +C GH K D+C + + IDL L V+ R L+ K + PN+ + E+I + ++ + +R LFGS+ NGF + SDLD+C+ + + L+ E+ + L + LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA + P VK LG +K+ AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G E P +DGW+A++ N + + ++ LWL F +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL L R +L L P + RV CF+C GH RDC + R AP GL PS++ L F
Sbjct: 622 SLSEEDLLYIFDKDILTSGKPPTIVCSICKRDGHAK----------DDCPEDFKKIDLKPLPPMTVRFREILDRVCKRCYDELSPNSSEQQNREQILAYLERFIRKEFNNHSRLCLFGSSKNGFGFRDSDLDICMTLEGHENAKKLNCKEIIDGLAKV---LKKHPGLKNILAITTAKVPIVKFEHKESGVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQETPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPIYPIPGLEREYFFDSKVLTDGELAPNDRCCRVCGKIETFELIKKKENEKDDDKDIKEDERDLREKRCFTCGDVGHVRRDCPENNPVRQKNGGIMAPQILQGLILPQPSVSLTLSF 1112 HSP 2 Score: 128.642 bits (322), Expect = 3.919e-29 Identity = 114/441 (25.85%), Postives = 205/441 (46.49%), Query Frame = 1
Query: 220 SLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHK--LAELQSQELSDVSVSRVLVNDTQL---------------SYVNGMQWIKQ------------------------LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLR 1416
+L + +S + L + S ++ +++CK+C H+++I A KH ++ HK+NI + +N L LP PT Q+ A+ L + KE + E++ R + M ++ + P L + + SD+N+ ++ + + Y+ ++ ++ +VE F +FCKD K G+T +SA N L +E + K++ L FRY+A ID +G PS F+++V+F+LQ+ PILP + +S++L D + V +D L S +G +Q +++ P E SL LW+ LLKFY ++F +++S+R + + +++ RRLAIEDPF +KRN+ NS V +++ ++ + + YFA PQ +
Sbjct: 38 ALTQMFTSPHPLHLFQRSPEYSNCQYLCKLCTVHIENIQGAHKHIKEKRHKKNILEKQEENELRALPPPTPAQISALGLALLDAAKE-NGVSQEDFLLRQEIVKSMEKIIQMKLPECSLRM-YGSSLTRFAFTSSDINIDVKFPRKVLIKLFYVLSIACYT---------EVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGV-SDDCYLDWEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKE-SLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERFRAAYRYFACPQCK 465 HSP 3 Score: 85.8853 bits (211), Expect = 4.631e-16 Identity = 50/180 (27.78%), Postives = 92/180 (51.11%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
GV IS N L N+ ++ Y ID R + L ++FA + I G S A+ ++V+++LQQ + P++P L ++ + Q + R++ + N + + + N S+ ELW+G L+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 798 GVEGDISLYNTLAQHNTRMLATYAAIDPRVKYLGYTVKFFAKRCDIGDASRGSLSSYAYILMVLYFLQQRNPPVIPVLQEIYDGQE------TPQRMVDGWNAFFFDNTEELRNRFPSLGKNRESVGELWLGFLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 971 HSP 4 Score: 82.0333 bits (201), Expect = 6.374e-15 Identity = 75/334 (22.46%), Postives = 146/334 (43.71%), Query Frame = 1
Query: 2434 IAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI-------------LQELY-EGERPTLYIDGWDAWYQTN--------------------ITIINKLWNKKSN-------------CNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ ++I + +I++ P +++GS++ F +SD+++ ++F +V +L + + ++ AKVP++ K + + +S N T+ L+ L P + L +Y A+ C I ++G + SY + LM++ FLQKR ILP L + + +G Y+D W+ +N T +++L ++K+ ++ +LWL+ +Y +EF +++ V+++R L + +KNW +A+EDPF L N+ SL+ + YILE F
Sbjct: 134 LRQEIVKSMEKIIQMKLPECSLRMYGSSLTRFAFTSSDINIDVKF----------PRKVLIKLFYV----LSIACYTEVESDFHAKVPVVFCKDKKSGLTCKVSAGNDTACLTTEFLEAIGKLEPVLIPLVMAFRYWARLCHIDCQAEGGIPSYCFALMVVFFLQKRQPPILPAYLGPWIDGFESKKLDDYHLKGVSDDCYLD-WEYRPSSNNENKGSSTTDGRGRTEQNHRKTAVSELESRKNKERQGRSALVFDHPPKESLGHLWLELLKFYTLEFAMEEHVISVRVQGLLSRDNKNWPRRRLAIEDPFALKRNVARSLNSQMVYEYILERF 452
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Match: Tut4 (terminal uridylyl transferase 4 [Source:MGI Symbol;Acc:MGI:2445126]) HSP 1 Score: 233.032 bits (593), Expect = 3.002e-61 Identity = 153/457 (33.48%), Postives = 237/457 (51.86%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLR------AELNAK--FKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + V+SD DE +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + +L K F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 891 CNCKATEVTSDLVDE------DNLPSQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1325 HSP 2 Score: 140.584 bits (353), Expect = 6.842e-33 Identity = 112/439 (25.51%), Postives = 204/439 (46.47%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQEL-----------SDVSVSRVLV-------------------NDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP+P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ K+ L + VE F + CKD K + +SA N + L ++L+ K++ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + S + + L+ D Q + L N++ L +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G
Sbjct: 302 ERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPSPSSAHLAALSVAVVELAKE-QGITDDDLRIRQDIVEEMSKVIMTFLPECSLRL-YGSSLTKFALKSSDVNIDIKF-PPKMNHPDLL--IQVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGG 735 HSP 3 Score: 85.8853 bits (211), Expect = 3.882e-16 Identity = 51/183 (27.87%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQ-LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K+ L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1081 RSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1256 HSP 4 Score: 80.4925 bits (197), Expect = 1.720e-14 Identity = 86/363 (23.69%), Postives = 156/363 (42.98%), Query Frame = 1
Query: 2347 SIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
S LA L EL AK + I +D +I + I +++++++ P +L+GS++ F K+SD+++ ++F N ++ +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + I NK + +SN + + LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 369 SAHLAALSVAVVEL-AKEQGIT--DDDLRIRQDIVEEMSKVIMTFLPECSLRLYGSSLTKFALKSSDVNIDIKF-------PPKMNHPDLLIQVLGI-LKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 720
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Match: Tut4 (terminal uridylyl transferase 4 [Source:MGI Symbol;Acc:MGI:2445126]) HSP 1 Score: 233.032 bits (593), Expect = 3.015e-61 Identity = 153/456 (33.55%), Postives = 237/456 (51.97%), Query Frame = 1
Query: 2125 NIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLR------AELNAK--FKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + V+SD DE +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + +L K F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 892 NCKATEVTSDLVDE------DNLPSQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1325 HSP 2 Score: 140.584 bits (353), Expect = 7.396e-33 Identity = 116/460 (25.22%), Postives = 211/460 (45.87%), Query Frame = 1
Query: 166 SAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQEL-----------SDVSVSRVLV-------------------NDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
+ VI++ E L E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP+P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ K+ L + VE F + CKD K + +SA N + L ++L+ K++ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + S + + L+ D Q + L N++ L +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G
Sbjct: 281 ATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPSPSSAHLAALSVAVVELAKE-QGITDDDLRIRQDIVEEMSKVIMTFLPECSLRL-YGSSLTKFALKSSDVNIDIKF-PPKMNHPDLL--IQVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGG 735 HSP 3 Score: 85.8853 bits (211), Expect = 3.917e-16 Identity = 51/181 (28.18%), Postives = 93/181 (51.38%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K +L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1083 GLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1256 HSP 4 Score: 80.1073 bits (196), Expect = 2.293e-14 Identity = 86/363 (23.69%), Postives = 156/363 (42.98%), Query Frame = 1
Query: 2347 SIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
S LA L EL AK + I +D +I + I +++++++ P +L+GS++ F K+SD+++ ++F N ++ +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + I NK + +SN + + LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 369 SAHLAALSVAVVEL-AKEQGIT--DDDLRIRQDIVEEMSKVIMTFLPECSLRLYGSSLTKFALKSSDVNIDIKF-------PPKMNHPDLLIQVLGI-LKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 720
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Match: Tut7 (terminal uridylyl transferase 7 [Source:MGI Symbol;Acc:MGI:2387179]) HSP 1 Score: 228.024 bits (580), Expect = 2.911e-60 Identity = 138/424 (32.55%), Postives = 216/424 (50.94%), Query Frame = 1
Query: 2242 RKLVFT--------CGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELN--------AKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
RKL FT C LC +GH K+ +C + + + L L L + + +K P + E I + +K +P + LFGS+ NGF K SDLDVC+ + + L + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P + +DGW+ ++ I + W + + LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 545 RKLTFTKGKSPTVVCSLCKREGHLKK----------DCPEDFKRVQLEPLPPLTPKFSNILDQVCVQCYKDFSPTIVEDQAREHIRQNLESFIKQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTINGHETAEGL---DCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKKPEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDNPSKLAY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 954 HSP 2 Score: 97.0561 bits (240), Expect = 1.075e-19 Identity = 65/248 (26.21%), Postives = 116/248 (46.77%), Query Frame = 1
Query: 760 SGLFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP----------NLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSL------------AELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKK 1437
SGL CK +SA N L ++ + K++ R L FRY+A ID+ ++G P F+++ +F+LQQ P+LP +L+KL +++ SV +++ + + + ++ L +LW+ LL+FY ++FN ++SIR E + + + +R+AIEDP+ +KRN+ N+ V +++ L+ + YFA+P T P K
Sbjct: 1 SGLLCK--------VSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFALPHKVTKPNLTK 240 HSP 3 Score: 78.5666 bits (192), Expect = 5.226e-14 Identity = 47/183 (25.68%), Postives = 90/183 (49.18%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNP-PANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ S P++P L ++ + + + +LV+ + + + + + W N + +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 707 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKK-------PEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 882 HSP 4 Score: 64.6994 bits (156), Expect = 8.799e-10 Identity = 55/231 (23.81%), Postives = 96/231 (41.56%), Query Frame = 1
Query: 2740 ISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI----------LQELYEGERPTLYIDGWDAWYQTNITI-------------------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECFAHILKLHSNSDNLNRPD 3336
+S N TK L L P + L +Y AK C I +G L Y + LM + FLQ+R +LP+ L +L + D Y N T + +N K ++ + LW++ +YA+EF++ V++IR + + + K+W +A+EDP+++ N+ +L+ + YIL C K + + +P+
Sbjct: 7 VSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFALPHKVTKPN 237
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Match: Tut7 (terminal uridylyl transferase 7 [Source:MGI Symbol;Acc:MGI:2387179]) HSP 1 Score: 226.868 bits (577), Expect = 1.938e-59 Identity = 138/424 (32.55%), Postives = 216/424 (50.94%), Query Frame = 1
Query: 2242 RKLVFT--------CGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELN--------AKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
RKL FT C LC +GH K+ +C + + + L L L + + +K P + E I + +K +P + LFGS+ NGF K SDLDVC+ + + L + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P + +DGW+ ++ I + W + + LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 947 RKLTFTKGKSPTVVCSLCKREGHLKK----------DCPEDFKRVQLEPLPPLTPKFSNILDQVCVQCYKDFSPTIVEDQAREHIRQNLESFIKQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTINGHETAEGL---DCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKKPEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDNPSKLAY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 1356 HSP 2 Score: 132.494 bits (332), Expect = 2.408e-30 Identity = 108/427 (25.29%), Postives = 194/427 (45.43%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQ-LKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP----------NLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSL------------AELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKK 1437
E L I L + P AK+ CK+C +D I A KH ++ HK+N+ + + LLT LP P Q+ AV ++ ++E + +E +R+ + M + R P L + SDVN+ ++ P + + L +++ KN + V+ F + C+DK G+ +SA N L ++ + K++ R L FRY+A ID+ ++G P F+++ +F+LQQ P+LP +L+KL +++ SV +++ + + + ++ L +LW+ LL+FY ++FN ++SIR E + + + +R+AIEDP+ +KRN+ N+ V +++ L+ + YFA+P T P K
Sbjct: 222 ERLKRDCIDRLKRRPRNCPTAKYTCKLCDALIDSIPFAHKHIKEKRHKKNLKEKQEEELLTTLPPPAPSQIHAVGSAIDRVVQE-FGLHSENLDQRLEIKRVMESVFRHKLPDCSLRL-YGSSCSRLGFRDSDVNIDVQF-PAVMSQPDVLLLVQE---CLKNSDSFIDVDADFHARVPVVVCRDKQSGLLCKVSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFALPHKVTKPNLTK 642 HSP 3 Score: 77.7962 bits (190), Expect = 9.348e-14 Identity = 47/183 (25.68%), Postives = 90/183 (49.18%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNP-PANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ S P++P L ++ + + + +LV+ + + + + + W N + +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 1109 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKK-------PEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 1284 HSP 4 Score: 76.2554 bits (186), Expect = 3.209e-13 Identity = 73/327 (22.32%), Postives = 140/327 (42.81%), Query Frame = 1
Query: 2500 QLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI----------LQELYEGERPTLYIDGWDAWYQTNITI-------------------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN 3384
+L+GS+ + + SD+++ ++F +S +V L + LK ++ +D A+VP++ K + + +S N TK L L P + L +Y AK C I +G L Y + LM + FLQ+R +LP+ L +L + D Y N T + +N K ++ + LW++ +YA+EF++ V++IR + + + K+W +A+EDP+++ N+ +L+ + YIL C K + + + + +P++ +PP+
Sbjct: 337 RLYGSSCSRLGFRDSDVNIDVQFPA-----VMSQPDV---LLLVQECLKNSDSFIDVDADFHARVPVVVCRDKQSGLLCKVSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFA----------LPHKVTKPNLTKPPS 645
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Match: Tut4 (terminal uridylyl transferase 4 [Source:MGI Symbol;Acc:MGI:2445126]) HSP 1 Score: 139.813 bits (351), Expect = 8.862e-33 Identity = 113/439 (25.74%), Postives = 207/439 (47.15%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQEL-----------SDVSVSRVLVND---------------TQLSYVNGMQWIKQLWNP----PANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP+P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ K+ L + VE F + CKD K + +SA N + L ++L+ K++ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + S + + L+ D T+ + K +P N++ L +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G
Sbjct: 263 ERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPSPSSAHLAALSVAVVELAKEQ-GITDDDLRIRQDIVEEMSKVIMTFLPECSLRL-YGSSLTKFALKSSDVNIDIKF-PPKMNHPDLL--IQVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGG 696 HSP 2 Score: 77.7962 bits (190), Expect = 8.310e-14 Identity = 86/363 (23.69%), Postives = 156/363 (42.98%), Query Frame = 1
Query: 2347 SIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
S LA L EL AK + I +D +I + I +++++++ P +L+GS++ F K+SD+++ ++F N ++ +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + I NK + +SN + + LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 330 SAHLAALSVAVVEL-AKEQGIT--DDDLRIRQDIVEEMSKVIMTFLPECSLRLYGSSLTKFALKSSDVNIDIKF-------PPKMNHPDLLIQVLGI-LKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 681
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Match: sp|B2RX14|TUT4_MOUSE (Terminal uridylyltransferase 4 OS=Mus musculus OX=10090 GN=Tut4 PE=1 SV=2) HSP 1 Score: 233.032 bits (593), Expect = 2.100e-60 Identity = 153/457 (33.48%), Postives = 237/457 (51.86%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLR------AELNAK--FKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + V+SD DE +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + +L K F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 891 CNCKATEVTSDLVDE------DNLPSQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1325 HSP 2 Score: 140.584 bits (353), Expect = 4.786e-32 Identity = 112/439 (25.51%), Postives = 204/439 (46.47%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQEL-----------SDVSVSRVLV-------------------NDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP+P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ K+ L + VE F + CKD K + +SA N + L ++L+ K++ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + S + + L+ D Q + L N++ L +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G
Sbjct: 302 ERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPSPSSAHLAALSVAVVELAKE-QGITDDDLRIRQDIVEEMSKVIMTFLPECSLRL-YGSSLTKFALKSSDVNIDIKF-PPKMNHPDLL--IQVLGILKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQKKGG 735 HSP 3 Score: 85.8853 bits (211), Expect = 2.715e-15 Identity = 51/183 (27.87%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQ-LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K+ L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1081 RSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1256 HSP 4 Score: 80.4925 bits (197), Expect = 1.203e-13 Identity = 86/363 (23.69%), Postives = 156/363 (42.98%), Query Frame = 1
Query: 2347 SIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
S LA L EL AK + I +D +I + I +++++++ P +L+GS++ F K+SD+++ ++F N ++ +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + I NK + +SN + + LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 369 SAHLAALSVAVVEL-AKEQGIT--DDDLRIRQDIVEEMSKVIMTFLPECSLRLYGSSLTKFALKSSDVNIDIKF-------PPKMNHPDLLIQVLGI-LKKSALYIDVESDFHAKVPVVVCKDRKSALLCRVSAGNDMACLTTDLLAALGKVEPVFTPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWEYNSSSATEKNLIADENKAKADEPKDDTKKTETDNQSNAAKAKHGKSPLTLEAPNQVPLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 720
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Match: sp|Q5VYS8|TUT7_HUMAN (Terminal uridylyltransferase 7 OS=Homo sapiens OX=9606 GN=TUT7 PE=1 SV=1) HSP 1 Score: 230.72 bits (587), Expect = 8.034e-60 Identity = 137/426 (32.16%), Postives = 221/426 (51.88%), Query Frame = 1
Query: 2191 LQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
+ + D Y F + I + C LC +GH K+ C R + L + +L L +K P + E I + ++ +P + LFGS+ NGF K SDLDVC+ + L++ + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P +++DGW+ ++ I + W++ ++ LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 941 VDQSDFFYEFSKLIFTKGKSPTVVCSLCKREGHLKKDCPEDFKRIQ--LEPLPPLTPKFLNILDQVCIQCYKDFSPTIIEDQAREHIRQNLESFIRQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTI---NGLETAEGLDCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKKPEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDYPSKMEY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 1360 HSP 2 Score: 113.235 bits (282), Expect = 1.032e-23 Identity = 119/456 (26.10%), Postives = 213/456 (46.71%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQ-LKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP----------NLHKLAELQSQELS-DVSV---SRVLVNDTQLSYVNG----------MQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA-FKKVNFVEITLSNLKPIANVTP-IFTKDN 1512
E L I L + +P AK+ C++C ++ I A KH ++ HK+NI + + LLT LP PT Q+ AV ++ ++E + NE +R+ + M + + P L + SDVN+ ++ P + + L +++ KN + V+ F + C++K G+ +SA N L ++ + K++ + L FRY+A ID+ ++G P F+++ IF+LQQ P+LP +L KL Q++ DV + + DT ++ + I + + P+ + + +LW+ LL+FY ++FN ++SIR E V + + +R+AIEDP+ +KRN+ NS V +++ L+ + YFA+P T + K +N + + K + N P + TKD+
Sbjct: 222 ERLKRDCIDRLKRRPRNYPTAKYTCRLCDVLIESIAFAHKHIKEKRHKKNIKEKQEEELLTTLPPPTPSQINAVGIAIDKVVQE-FGLHNENLEQRLEIKRIMENVFQHKLPDCSLRL-YGSSCSRLGFKNSDVNIDIQF-PAIMSQPDVLLLVQE---CLKNSDSFIDVDADFHARVPVVVCREKQSGLLCKVSAGNENACLTTKHLTALGKLEPKLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAIFFLQQRKEPLLPVYLGSWIEGFSLSKLGNFNLQDIEKDVVIWEHTDSAAGDTGITKEEAPRETPIKRGQVSLILDVKHQPS--VPVGQLWVELLRFYALEFNLADLVISIRVKELVSRELKDWPKKRIAIEDPYSVKRNVARTLNSQPVFEYILHCLRTTYKYFALPHKITKSSLLKPLNAITCISEHSKEVINHHPDVQTKDD 669 HSP 3 Score: 78.5666 bits (192), Expect = 4.763e-13 Identity = 46/183 (25.14%), Postives = 91/183 (49.73%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ + P++P L ++ + + + + V+ + + + + + W+ N S+ +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 1113 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIPVLQEIYKGEKK-------PEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 1288
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Match: sp|Q5TAX3|TUT4_HUMAN (Terminal uridylyltransferase 4 OS=Homo sapiens OX=9606 GN=TUT4 PE=1 SV=3) HSP 1 Score: 228.409 bits (581), Expect = 5.339e-59 Identity = 149/457 (32.60%), Postives = 233/457 (50.98%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNA--------KFKSIVPNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNS--DNLNRPDNVHY--MLLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
N K + +SD D+ +NL Q+L Y+FD+ I + C +C GH K ++C + IDL L + F + P E+I + + ++ Y AR LFGS+ NGF + SDLD+C+ + + L+ E+ L I LK+ ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA + P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G++ P +DGW+A++ + K ++ LWL +Y EFD + V++IRQ K L +K W S +A+EDPF+L+HNL +S + +I++ F + KL + R + +L L P N R C C K GH+ +DC
Sbjct: 874 CNCKATEDASDLNDD------DNLPTQELYYVFDKFILTSGKPPTIVCSICKKDGHSK----------NDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEIIENLAKI---LKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEIFDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLIGREAEYFFDSRVLTDGELAP---NDRCCRVCGKIGHYMKDC 1308 HSP 2 Score: 139.813 bits (351), Expect = 8.810e-32 Identity = 125/483 (25.88%), Postives = 224/483 (46.38%), Query Frame = 1
Query: 166 SAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKD-KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNL----------HKLAELQSQELSDVSVSRVLVNDTQLSYVNGM-QWIKQLWNPPANE-----------------------------MSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPA----FKKVNFVEITLSNLKPI 1479
+ VI++ E L E L +I L K S ++ +++CK+C H+++I A KH ++ HK+NI + ++ L LP P+ L A++ + KE I +++ R + +M+++ P L + + SDVN+ ++ P + L ++ ++ KN L + VE F + C+D K G+ +SA N + L ++L+ KI+ L FRY+A +ID DG PS F+++V+F+LQQ P+LP L ++ + Q + + + + N + + N + + K + P ++ +SL +LW+ LLKFY +DF Y++ +R + + + +++ RR+AIEDPF +KRN+ NS V +++ ++ + + YFA PQ + G FKK +I SN KP+
Sbjct: 261 ATVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIELAKE-HGITDDDLRVRQEIVEEMSKVITTFLPECSLRL-YGSSLTRFALKSSDVNIDIKF-PPKMNHPDLL--IKVLGILKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKI--SNKKPV 736 HSP 3 Score: 85.8853 bits (211), Expect = 2.372e-15 Identity = 51/183 (27.87%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ ++ Y ID R + L + FA + I G S A+ ++V+++LQQ P++P L ++ + + +V+ + + + +K +L + N SL ELW+GLL+FY +F+ Y++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 1064 RSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI-------FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKEYVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNL 1239 HSP 4 Score: 82.8037 bits (203), Expect = 2.588e-14 Identity = 77/339 (22.71%), Postives = 149/339 (43.95%), Query Frame = 1
Query: 2419 NDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILP-ILQELYEGERPTLYID------------GWD-----AWYQTNITIINKL-------------WNKKSNC----------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+D ++ ++I +++++++ P +L+GS++ F K+SD+++ ++F N +K +G LKK ++ AKVP++ + + + +S N + T +L + P L +Y AK C I + G + SY + LM++ FLQ+R +LP +L EG P D W+ A + +I NK + +SN +++ LWL+ +Y ++F +++ V+ +R L + +KNW +A+EDPF++ N+ SL+ + Y++E F
Sbjct: 370 DDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSSDVNIDIKF-------PPKMNHPDLLIKVLGI-LKKNVLYVDVESDFHAKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERF 700
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Match: sp|Q5BLK4|TUT7_MOUSE (Terminal uridylyltransferase 7 OS=Mus musculus OX=10090 GN=Tut7 PE=1 SV=3) HSP 1 Score: 226.868 bits (577), Expect = 1.527e-58 Identity = 138/424 (32.55%), Postives = 216/424 (50.94%), Query Frame = 1
Query: 2242 RKLVFT--------CGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELN--------AKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECF---AHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
RKL FT C LC +GH K+ +C + + + L L L + + +K P + E I + +K +P + LFGS+ NGF K SDLDVC+ + + L + ++ + R L+K + ++ I I AKVPI+KF+ + ++ DIS+ N+L L NT++L Y+ + P VK L +K K C+IGDAS+G+LSSYAY LM+++FLQ+R ++P+LQE+Y+GE +P + +DGW+ ++ I + W + + LWL +Y EFD + V++IR+ L K W S Y+ +EDPF+L+HNL LS + +I++ F + + + P + Y +P VL N R C C K GHF +DC
Sbjct: 947 RKLTFTKGKSPTVVCSLCKREGHLKK----------DCPEDFKRVQLEPLPPLTPKFSNILDQVCVQCYKDFSPTIVEDQAREHIRQNLESFIKQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTINGHETAEGL---DCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKKPEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDNPSKLAY-FFDPDVLTEGELAPNDRCCRICGKIGHFMKDC 1356 HSP 2 Score: 132.109 bits (331), Expect = 1.879e-29 Identity = 108/427 (25.29%), Postives = 194/427 (45.43%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQ-LKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP----------NLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSL------------AELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKK 1437
E L I L + P AK+ CK+C +D I A KH ++ HK+N+ + + LLT LP P Q+ AV ++ ++E + +E +R+ + M + R P L + SDVN+ ++ P + + L +++ KN + V+ F + C+DK G+ +SA N L ++ + K++ R L FRY+A ID+ ++G P F+++ +F+LQQ P+LP +L+KL +++ SV +++ + + + ++ L +LW+ LL+FY ++FN ++SIR E + + + +R+AIEDP+ +KRN+ N+ V +++ L+ + YFA+P T P K
Sbjct: 222 ERLKRDCIDRLKRRPRNCPTAKYTCKLCDALIDSIPFAHKHIKEKRHKKNLKEKQEEELLTTLPPPAPSQIHAVGSAIDRVVQE-FGLHSENLDQRLEIKRVMESVFRHKLPDCSLRL-YGSSCSRLGFRDSDVNIDVQF-PAVMSQPDVLLLVQE---CLKNSDSFIDVDADFHARVPVVVCRDKQSGLLCKVSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFALPHKVTKPNLTK 642 HSP 3 Score: 77.7962 bits (190), Expect = 6.901e-13 Identity = 47/183 (25.68%), Postives = 90/183 (49.18%), Query Frame = 1
Query: 781 KLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNP-PANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
+ G+ IS N L N+ L+ Y ID R + L + F I G S A++++V+++LQQ S P++P L ++ + + + +LV+ + + + + + W N + +LW+GLL+FY +F+ +++SIR + + ++ + IEDPFD+ N+
Sbjct: 1109 RSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKK-------PEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLWLGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNL 1284 HSP 4 Score: 76.2554 bits (186), Expect = 2.492e-12 Identity = 73/327 (22.32%), Postives = 140/327 (42.81%), Query Frame = 1
Query: 2500 QLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI----------LQELYEGERPTLYIDGWDAWYQTNITI-------------------INKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN 3384
+L+GS+ + + SD+++ ++F +S +V L + LK ++ +D A+VP++ K + + +S N TK L L P + L +Y AK C I +G L Y + LM + FLQ+R +LP+ L +L + D Y N T + +N K ++ + LW++ +YA+EF++ V++IR + + + K+W +A+EDP+++ N+ +L+ + YIL C K + + + + +P++ +PP+
Sbjct: 337 RLYGSSCSRLGFRDSDVNIDVQFPA-----VMSQPDV---LLLVQECLKNSDSFIDVDADFHARVPVVVCRDKQSGLLCKVSAGNENAWLTTKHLTALGKLEPRLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKKGQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRNVARTLNNQPVFEYILHCLRTTYKYFA----------LPHKVTKPNLTKPPS 645
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Match: sp|O64642|URT1_ARATH (UTP:RNA uridylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=URT1 PE=1 SV=2) HSP 1 Score: 143.28 bits (360), Expect = 2.127e-33 Identity = 110/342 (32.16%), Postives = 173/342 (50.58%), Query Frame = 1
Query: 2392 AKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPILQELYEGERPT--LYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNW------QSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPNG 3387
A +KS++P + + ++ + +V +P A+ L+GS N F SD+DVCL DD ++ +E+ +L E+ + + +Q + + +A+VPI+K I DI INN L + NTK+L+ YA + ++ L ++K+ AKS + + QGTLSSYAY+LM IHFLQ+R ILP LQE+ PT + +D Y N +++L N SN I L FF Y+A D VV++R L K +K+W + +EDPF SH+L + + R + E F ++ +++ N LLEP + NG
Sbjct: 440 AIYKSLIPAEEELEKQRQLMAHLENLVAKEWPHAKLYLYGSCANSFGFPKSDIDVCLAIEGDD----INKSEMLLKLA----EILESDNLQNVQALTRARVPIVKLMDPVTGISCDICINNVLAVVNTKLLRDYAQIDVRLRQLAFIVKHWAKSRRVNETYQGTLSSYAYVLMCIHFLQQRRPPILPCLQEM----EPTYSVRVDNIRCTYFDN---VDRLRNFGSNNRETIAELVWGFFNYWAYAHDYAYNVVSVRTGSILGKREKDWTRRVGNDRHLICIEDPFETSHDLGRVVDKFSIRVLREEFERAARI------MHQDPNPCAKLLEPYIPEDNNG 760 HSP 2 Score: 63.929 bits (154), Expect = 1.032e-8 Identity = 47/186 (25.27%), Postives = 92/186 (49.46%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSD----RHIVNRR--LAIEDPFDIKRNI 1326
G++ I +N L +N++L+R Y +ID R LA + +++A +++ G S A+ ++ I +LQQ PILP L ++ S V V++ + +Y + + ++ + N ++AEL G ++ + + +VS+R + K + R + N R + IEDPF+ ++
Sbjct: 543 GISCDICINNVLAVVNTKLLRDYAQIDVRLRQLAFIVKHWAKSRRVNETYQGTLSSYAYVLMCIHFLQQRRPPILPCLQEMEPTYS----------VRVDNIRCTYFDNVDRLRNFGS--NNRETIAELVWGFFNYWAYAHDYAYNVVSVRTGSILGKREKDWTRRVGNDRHLICIEDPFETSHDL 716
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Match: A0A1A8FSG1 (Zinc finger, CCHC domain containing 6 (Fragment) OS=Nothobranchius korthausae OX=1143690 GN=ZCCHC6 PE=4 SV=1) HSP 1 Score: 252.292 bits (643), Expect = 3.423e-68 Identity = 144/438 (32.88%), Postives = 228/438 (52.05%), Query Frame = 1
Query: 2149 SDNPDEYNESQVENLQKQDLVYLFDRISI-HNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM--LLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
S P + + ++ + F R + + C LC GH K+ C + + + L + +L L + + PN+ + E I + V+ + AR +LFGS+ NGF + SDLD+C+ D L +L + +N +K + R LKK ++ I I AKVPI+KF+ + ++GDIS+ N+LGL NT +L YA + VK L IK AK+C+IGDAS+G+LSSYAY LM++ FLQ+R ++P+LQE+Y+G++P + IDGW+ ++ ++ + +W + + LWL +Y +FD + VV IRQ +L +K W S Y+ +EDPF+L+HNL LS + +I++ F + +L + P M + VL N R C C K GH+ +DC
Sbjct: 17 SPGPSKKRPEEESTPKQTRSAFEFTRQAFTRGKSHTVVCSLCKRDGHLKKDCPEDFKKVE--LEPLPPMTPEFLRVLSSVCGRCYTDFSPNDLEQDVKESILQDLETFVRKQFSGARLRLFGSSKNGFGFRLSDLDICMVLEGRDNLHNL---DCTNIIKGLARHLKKHPALRNILPITTAKVPIVKFFHRIAGLEGDISLYNTLGLHNTHLLATYAAIDTRVKALCYAIKVFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEMYDGKKPKVLIDGWNVYFHDDLKTLPSVWPEYGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRLFGTPVKMQLPIAPSAMEYFFDAEVLTGGECAPNDRCCRICGKIGHYMKDC 449 HSP 2 Score: 90.8929 bits (224), Expect = 9.205e-15 Identity = 53/181 (29.28%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNP-PANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ L+ Y ID+R + L + FA I G S A++++V+F+LQQ + P++P L QE+ D +VL++ + + + ++ + +W N ++ ELW+GLL+FY DF+ ++V IR + ++ ++ + IEDPFD+ N+
Sbjct: 205 GLEGDISLYNTLGLHNTHLLATYAAIDTRVKALCYAIKVFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVL--------QEMYDGKKPKVLIDGWNVYFHDDLKTLPSVWPEYGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNL 377
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Match: A7RX54 (Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g96536 PE=4 SV=1) HSP 1 Score: 245.358 bits (625), Expect = 1.459e-67 Identity = 150/428 (35.05%), Postives = 234/428 (54.67%), Query Frame = 1
Query: 2206 LVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICN-------YAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVS--NRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYML----LEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+VY FD I VFTC C GH KE C A+PR + + ++I A +++ + N+ + +++ + + ++ +YP A LFGS++NGF K SDLD+C+ L + ++V + + ++LK+ + ++ + I AKVPI+KFY+++ +GDIS+ N+L L+N++ML+ YADL V+ LG +K AK C+IGDAS+G+LSSYAY+LML+H+LQ + ++PILQEL+ G+ P I+GW+ WY ++ + K+W K+ ++ + LWL F YY FD + +VV R+SK+L K +K W A+EDPFNLSHNL ++ + A +I F + L Y L L P + N RV C + GH A+DC
Sbjct: 3 IVYKFDSSILTGGHAPVFTCCYCKKDGHIKEKCPDLKKPVIVALPRMTK---VYQNIIDAACIRVGETMGL-------NHMEGQFRQEVLRNLEDYIREVYPAACLYLFGSSVNGFGFKESDLDICMT------LDGKTKDDVDPIKVIHDLSKKLKQHSDIRNVLAITTAKVPIVKFYIRSVKREGDISLYNTLALENSRMLRTYADLDVRVRQLGFTLKIFAKVCDIGDASKGSLSSYAYILMLLHYLQTCQPPVIPILQELHNGQCPNNMIEGWNCWYYNDLPNLPKVWKSKNRESVGL--LWLGFLRYYTETFDWEHDVVCCRRSKRLTKFEKMWTKHEFAIEDPFNLSHNLGAGITRKMATFISLVFRRGRERFGTPIQLPLQLYDEYFFNVDYLTDGALAPCDRNCRV---CGRIGHLAKDC 409 HSP 2 Score: 72.4034 bits (176), Expect = 3.403e-9 Identity = 47/175 (26.86%), Postives = 79/175 (45.14%), Query Frame = 1
Query: 802 ISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
IS N L NS ++R Y +D R L + FA I G S A+ ++++ YLQ P++P L QEL + ++ Y N + + ++W N S+ LW+G L++Y F+ +V R ++ + K ++ AIEDPF++ N+
Sbjct: 175 ISLYNTLALENSRMLRTYADLDVRVRQLGFTLKIFAKVCDIGDASKGSLSSYAYILMLLHYLQTCQPPVIPIL--------QELHNGQCPNNMIEGWNCWYYNDLPNLPKVWK-SKNRESVGLLWLGFLRYYTETFDWEHDVVCCRRSKRLTKFEKMWTKHEFAIEDPFNLSHNL 340
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Match: A0A3Q2E3J3 (Uncharacterized protein OS=Cyprinodon variegatus OX=28743 PE=4 SV=1) HSP 1 Score: 253.447 bits (646), Expect = 1.609e-67 Identity = 154/465 (33.12%), Postives = 246/465 (52.90%), Query Frame = 1
Query: 2122 VNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDRISI-HNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM--LLEPSVLRPPN--GNMRVCFSCNKQGHFARDCVDKGNKRTPTKAAPNQQ 3492
V+++ SA S + E + + Q+ Y F + + + + C LC GHRK+ C + + D L + +L L ++ VP++ + E I + V+ +P AR QLFGS+ NGF + SDLD+C+ V ++ +++ + +K + R+LKK ++ I I AKVPI+KFY + ++GDIS+ N+L L NT++L YA + VK+L ++K AK C+IGDAS+G+LSSYAY LM++ FLQ+R ++P+LQE+Y+G+ +P + +DGW+ ++ ++ + W + LWL +Y EFD + VV IRQ +L +K W S Y+ +EDPF+L+HNL LS +I++ F + ++ + P M +P VL N R C C K GHF +DC + NK P P +Q
Sbjct: 167 VHVRRSASPSQSTTENESTDNKTQQQSRRAYKFTKQAFTKGKSHMVVCSLCKRDGHRKKDCPEDFTKME--LDPLPLLTPDFLHVLNRVCEQCYRDFVPDDQETGVRELILQDLQSFVRRQFPGARLQLFGSSKNGFGFRRSDLDICM---VLEEKETIDEFDCIIIIKGLARQLKKHQGLKNILPITTAKVPIVKFYHIHTGLEGDISLYNTLALYNTRLLASYAAIDRRVKILCYVMKVFAKICDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKTKPEVLVDGWNVFFYEDLKSLPLYWPHCGKNTETVGELWLGLLRFYTEEFDFREHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNLGAGLSRRMTNFIMKAFINGRRVFGTPVRVFPPIYPTEMEYFFDPEVLTEGKVAPNDRCCRVCGKIGHFLKDCPMR-NKWVPFLIRPKKQ 625 HSP 2 Score: 81.6481 bits (200), Expect = 9.088e-12 Identity = 50/181 (27.62%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLW-NPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ L+ Y ID R + L + + FA I G S A++++V+F+LQQ + P++P L ++ + +++ VLV+ + + ++ + W + N ++ ELW+GLL+FY +F+ ++V IR + ++ ++ + IEDPFD+ N+
Sbjct: 364 GLEGDISLYNTLALYNTRLLASYAAIDRRVKILCYVMKVFAKICDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKTK-------PEVLVDGWNVFFYEDLKSLPLYWPHCGKNTETVGELWLGLLRFYTEEFDFREHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNL 537
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Match: A0A1A8QG98 (Zinc finger, CCHC domain containing 6 (Fragment) OS=Nothobranchius rachovii OX=451742 GN=ZCCHC6 PE=4 SV=1) HSP 1 Score: 248.054 bits (632), Expect = 1.077e-66 Identity = 145/442 (32.81%), Postives = 229/442 (51.81%), Query Frame = 1
Query: 2146 SSDNPDEYNESQVENLQKQDLVYLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM-------LLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
S P+E N + + F R H C LC GH K+ C + + + L + +L L + + P++ + E I + V+ + AR +LFGS+ NGF + SDLD+C+ D L +L + +N +K + R LKK ++ I I AKVPI+KF+ + ++GDIS+ N+LGL NT +L YA + VK+L +K AK+C+IGDAS+G+LSSYAY LM++ FLQ+R ++P+LQE+Y+G ++P + +DGW+ ++ ++ + +W + + LWL +Y +FD + VV IRQ +L +K W S Y+ +EDPF+L+HNL LS + +I++ F + +L + P M +L L P N R C C K GH+ +DC
Sbjct: 20 SKKRPEEENPPKQTRSAFEFTRQAFTRGKSH----TVVCSLCKRDGHLKKDCPEDFKKVE--LEPLPPMTPEFLRVLSSVCGRCYTDFSPDDLEQDVKESILQDLEAFVRKQFSGARLRLFGSSKNGFGFRMSDLDICMVLEGRDNLHNL---DCTNIIKGLARHLKKHPALRNILPITTAKVPIVKFFHRFTGLEGDISLYNTLGLHNTHLLATYAAIDTRVKVLCYAMKIFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKKKPKVLVDGWNVYFHDDLKALPSVWPEHGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRLFGTPVKMQLPIAPSAMEYFFDTEVLTGGELAP---NDRCCRICGKIGHYMKDC 449 HSP 2 Score: 87.0409 bits (214), Expect = 1.551e-13 Identity = 51/181 (28.18%), Postives = 95/181 (52.49%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ L+ Y ID+R + L + FA I G S A++++V+F+LQQ + P++P L ++ + + + +VLV+ + + + ++ + +W N ++ ELW+GLL+FY DF+ ++V IR + ++ ++ + IEDPFD+ N+
Sbjct: 204 GLEGDISLYNTLGLHNTHLLATYAAIDTRVKVLCYAMKIFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKKK-------PKVLVDGWNVYFHDDLKALPSVWPEHGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNL 377
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Match: A0A1A8PK66 (Zinc finger, CCHC domain containing 6 (Fragment) OS=Nothobranchius rachovii OX=451742 GN=ZCCHC6 PE=4 SV=1) HSP 1 Score: 248.054 bits (632), Expect = 1.128e-66 Identity = 145/442 (32.81%), Postives = 229/442 (51.81%), Query Frame = 1
Query: 2146 SSDNPDEYNESQVENLQKQDLVYLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEG-ERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM-------LLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
S P+E N + + F R H C LC GH K+ C + + + L + +L L + + P++ + E I + V+ + AR +LFGS+ NGF + SDLD+C+ D L +L + +N +K + R LKK ++ I I AKVPI+KF+ + ++GDIS+ N+LGL NT +L YA + VK+L +K AK+C+IGDAS+G+LSSYAY LM++ FLQ+R ++P+LQE+Y+G ++P + +DGW+ ++ ++ + +W + + LWL +Y +FD + VV IRQ +L +K W S Y+ +EDPF+L+HNL LS + +I++ F + +L + P M +L L P N R C C K GH+ +DC
Sbjct: 22 SKKRPEEENPPKQTRSAFEFTRQAFTRGKSH----TVVCSLCKRDGHLKKDCPEDFKKVE--LEPLPPMTPEFLRVLSSVCGRCYTDFSPDDLEQDVKESILQDLEAFVRKQFSGARLRLFGSSKNGFGFRMSDLDICMVLEGRDNLHNL---DCTNIIKGLARHLKKHPALRNILPITTAKVPIVKFFHRFTGLEGDISLYNTLGLHNTHLLATYAAIDTRVKVLCYAMKIFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKKKPKVLVDGWNVYFHDDLKALPSVWPEHGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRLFGTPVKMQLPIAPSAMEYFFDTEVLTGGELAP---NDRCCRICGKIGHYMKDC 451 HSP 2 Score: 87.0409 bits (214), Expect = 1.561e-13 Identity = 51/181 (28.18%), Postives = 95/181 (52.49%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ L+ Y ID+R + L + FA I G S A++++V+F+LQQ + P++P L ++ + + + +VLV+ + + + ++ + +W N ++ ELW+GLL+FY DF+ ++V IR + ++ ++ + IEDPFD+ N+
Sbjct: 206 GLEGDISLYNTLGLHNTHLLATYAAIDTRVKVLCYAMKIFAKTCDIGDASRGSLSSYAYTLMVLFFLQQRNPPVIPVLQEIYDGKKK-------PKVLVDGWNVYFHDDLKALPSVWPEHGKNTETVGELWLGLLRFYTEDFDFKEHVVCIRQHARLTTFNKQWTSKYIVIEDPFDLNHNL 379
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Match: TUT4 (terminal uridylyltransferase 4 [Source:NCBI gene;Acc:103031850]) HSP 1 Score: 236.498 bits (602), Expect = 1.666e-62 Identity = 148/435 (34.02%), Postives = 228/435 (52.41%), Query Frame = 1
Query: 2200 QDLVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIV------------PNNDRPKIAEKIFDKITEIVKALY-PIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPILQELYEGER-PTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM----LLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+DL Y+FDR I + C +C GH K D+C + + ++LA L ++ +F+ I+ P + E+I + ++ Y A+ LFGS+ NGF + SDLD+C+ D + L+ E+ L I LKK ++ I I AKVPI+KF K + ++GDIS+ N+L NT+ML YA L P V+LLG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+Y+G P +DGW+A++ ++ + ++ + LWL +Y EFD + V++IRQ K+L +K W S +A+EDPF+L+HNL +S + +I++ F + KL Y +L L P N R C C K GH+ +DC
Sbjct: 743 EDLHYIFDRMIFTGGKPPTVVCSICKRDGHLK----------DDCPEDFKKMELAPL----PPMSERFREILDNLCKLCYHELSPTLGEQQKREQILCSLERFIRKEYNDKAQLCLFGSSKNGFGFRDSDLDICMTLEGHDTAEKLNCKEIIEGLAKI---LKKHTGLRNILPITTAKVPIVKFEHKQSGLEGDISLYNTLAQHNTRMLATYAALDPRVQLLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVLQEIYDGNTMPQRMVDGWNAFFFDDLEELRLRLSEFQKNRETVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPQPGMEAEYFFDSKVLTDGELAP---NDRCCRICGKIGHYMKDC 1157 HSP 2 Score: 162.155 bits (409), Expect = 1.342e-39 Identity = 122/432 (28.24%), Postives = 207/432 (47.92%), Query Frame = 1
Query: 229 ETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLII---CXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKDKL-GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILP---------------NLHKLAELQSQEL-----------SDVSVSRVLVNDTQLSYV----NGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTG 1422
E L YI L K S ++P +++CK+C H+D+I A KH ++ HK+NI + +N L LP P+ Q+ AV+ + ++ I EE+ +R G+ +M ++ ++ P L + C + + DV P N+ + L ++ ++ K+ +VE F +FC+D G+ +SA N + L + + +++ R L FRY+A +D +G PS +FS++VIF+LQQ PILP + + L +Q++ L SD +TQ +Q +L +SL +LW+ LL+FY ++F Y++SIR E + + ++ RRLAIEDPF +KRN+ NS V +++ ++ ++ + YFA PQ R G
Sbjct: 187 ERLQRDYIHRLAKQSPEYPNFQYLCKLCSVHVDNIQGAHKHIKEKRHKKNIMEKQEENELRALPAPSPAQMRAVDAAVMEAARQ-NGISEEEFQQRQGVLKRMQQIIQKHLPTCSLRLYGSCLTRFAFKTSDLNIDVAY-----PPNMTQPDVL--IQVLDILKKSAEFSEVESDFHAKVPVVFCRDAASGLPCKVSAGNDVACLTTNHLAALARLEPRLVPLVLAFRYWAKLCHVDCQAEGGIPSYSFSLMVIFFLQQRKQPILPVYLGCWIEGFDVKRVDEYHLTGVQTEGLYVGWEHRPGSASDGRRDSRPKPETQKREAEKKSENVQGKTRLVLEKTESVSLGQLWLELLRFYTLEFALEDYIISIRLKELLPRDAKNWPRRRLAIEDPFALKRNVARSLNSQMVFEYIQERFRMAYRYFACPQSRNG 610 HSP 3 Score: 89.3521 bits (220), Expect = 2.827e-17 Identity = 79/322 (24.53%), Postives = 139/322 (43.17%), Query Frame = 1
Query: 2440 EKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRG--ILPI-----------------------LQELYEG--ERPTLYIDGWDAWYQTNITIINKLWNKKSNC------------NINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENA-RYILECF 3285
+ + ++ +I++ P +L+GS + F K SDL++ + + + ++ +V ++ I LKK ++ AKVP++ + + +S N + T L A L P + L +Y AK C + ++G + SY++ LM+I FLQ+R ILP+ + LY G RP DG T + K N ++++ LWL+ +Y +EF ++ +++IR + L + KNW +A+EDPF L N+ SL+ + YI E F
Sbjct: 282 QGVLKRMQQIIQKHLPTCSLRLYGSCLTRFAFKTSDLNIDVAYPPN-----MTQPDVLIQVLDI---LKKSAEFSEVESDFHAKVPVVFCRDAASGLPCKVSAGNDVACLTTNHLAALARLEPRLVPLVLAFRYWAKLCHVDCQAEGGIPSYSFSLMVIFFLQQRKQPILPVYLGCWIEGFDVKRVDEYHLTGVQTEGLYVGWEHRPGSASDGRRDSRPKPETQKREAEKKSENVQGKTRLVLEKTESVSLGQLWLELLRFYTLEFALEDYIISIRLKELLPRDAKNWPRRRLAIEDPFALKRNVARSLNSQMVFEYIQERF 595 HSP 4 Score: 79.337 bits (194), Expect = 2.839e-14 Identity = 50/188 (26.60%), Postives = 98/188 (52.13%), Query Frame = 1
Query: 769 FCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDV-SVSRVLVNDTQLSYVNGMQWIK-QLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
F + G+ IS N L N+ ++ Y +D R + L + FA + I G S A+ ++V+++LQQ P++P L QE+ D ++ + +V+ + + ++ ++ +L N ++ ELW+GLL+FY +F+ +++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 909 FEHKQSGLEGDISLYNTLAQHNTRMLATYAALDPRVQLLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVL--------QEIYDGNTMPQRMVDGWNAFFFDDLEELRLRLSEFQKNRETVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTSKCIAIEDPFDLNHNL 1088
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Match: tent2 (terminal nucleotidyltransferase 2 [Source:NCBI gene;Acc:103039892]) HSP 1 Score: 124.405 bits (311), Expect = 3.115e-29 Identity = 87/255 (34.12%), Postives = 149/255 (58.43%), Query Frame = 1
Query: 2470 VKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQK--RGILPILQELY-EGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDK-NWQSAYMAVEDPFN 3222
++ L+P AR L GS++NGF +++SD D+CL V D + + V L + R L KL+ ++R +++AKVPI+KF + + ++ D+++NN +G++NT +L+ YA + V+ + +IK A +I DAS+GTLSSY +LM++H+LQ ++P LQ+ Y E P + I + ++K N+ S + L L F YYA F DK V+++R+ K L + + W++ ++ VE+PF+
Sbjct: 203 IQLLHPCARVFLAGSSLNGFGSRSSDADLCL--VVQDGPVNQRTDAVYI-LSRVQRLLYKLSYIERPQ-LIRAKVPILKFRDRISGVEFDLNVNNVVGIRNTFLLRTYAHIERRVRPIILVIKKWASHNKINDASRGTLSSYTLVLMVLHYLQTLPEPVIPCLQKDYPECFSPVMDIHLVPDGPRNIPPFVSK--NQSS-----LGDLLLGFLKYYATVFRWDKFVISVREGKALPRPNSWEWKNKFICVEEPFD 446
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Match: tent2 (terminal nucleotidyltransferase 2 [Source:NCBI gene;Acc:103039892]) HSP 1 Score: 124.405 bits (311), Expect = 3.115e-29 Identity = 87/255 (34.12%), Postives = 149/255 (58.43%), Query Frame = 1
Query: 2470 VKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQK--RGILPILQELY-EGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDK-NWQSAYMAVEDPFN 3222
++ L+P AR L GS++NGF +++SD D+CL V D + + V L + R L KL+ ++R +++AKVPI+KF + + ++ D+++NN +G++NT +L+ YA + V+ + +IK A +I DAS+GTLSSY +LM++H+LQ ++P LQ+ Y E P + I + ++K N+ S + L L F YYA F DK V+++R+ K L + + W++ ++ VE+PF+
Sbjct: 203 IQLLHPCARVFLAGSSLNGFGSRSSDADLCL--VVQDGPVNQRTDAVYI-LSRVQRLLYKLSYIERPQ-LIRAKVPILKFRDRISGVEFDLNVNNVVGIRNTFLLRTYAHIERRVRPIILVIKKWASHNKINDASRGTLSSYTLVLMVLHYLQTLPEPVIPCLQKDYPECFSPVMDIHLVPDGPRNIPPFVSK--NQSS-----LGDLLLGFLKYYATVFRWDKFVISVREGKALPRPNSWEWKNKFICVEEPFD 446
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Match: tent2 (terminal nucleotidyltransferase 2 [Source:NCBI gene;Acc:103039892]) HSP 1 Score: 119.013 bits (297), Expect = 1.228e-27 Identity = 85/251 (33.86%), Postives = 145/251 (57.77%), Query Frame = 1
Query: 2470 VKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQK--RGILPILQELY-EGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDK-NWQSAYMAVE 3210
++ L+P AR L GS++NGF +++SD D+CL V D + + V L + R L KL+ ++R +++AKVPI+KF + + ++ D+++NN +G++NT +L+ YA + V+ + +IK A +I DAS+GTLSSY +LM++H+LQ ++P LQ+ Y E P + I + ++K N+ S + L L F YYA F DK V+++R+ K L + + W++ ++ VE
Sbjct: 203 IQLLHPCARVFLAGSSLNGFGSRSSDADLCL--VVQDGPVNQRTDAVYI-LSRVQRLLYKLSYIERPQ-LIRAKVPILKFRDRISGVEFDLNVNNVVGIRNTFLLRTYAHIERRVRPIILVIKKWASHNKINDASRGTLSSYTLVLMVLHYLQTLPEPVIPCLQKDYPECFSPVMDIHLVPDGPRNIPPFVSK--NQSS-----LGDLLLGFLKYYATVFRWDKFVISVREGKALPRPNSWEWKNKFICVE 442
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Match: tut1 (speckle targeted PIP5K1A-regulated poly(A) polymerase-like [Source:NCBI gene;Acc:103029312]) HSP 1 Score: 88.5817 bits (218), Expect = 2.829e-17 Identity = 82/318 (25.79%), Postives = 141/318 (44.34%), Query Frame = 1
Query: 2506 FGSTINGFMTKASDLDVCLEFYVDDKLQSLS---------NNEVSNRLKTIGRELKKLNT--------------------VQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT---LSSYAYMLMLIHFLQKRG--ILPILQEL--YEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQL----------------------FKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYIL-EC 3282
FGS++N F ++ DLD+ L+ QS + N+E + +I E+ LNT V ++ ++P+++F ++ N+QGDI+INN L ++NT+ L++ + L V+ L +++ AK + G L++YA L+++ +LQ ILP +++L E IDGWD + + + N C L FF +Y+ +FD VV++R+ + L K + + + DPFNL HN+ G+LS R++L EC
Sbjct: 202 FGSSVNTFGVQSCDLDLVLDLENTKAFQSQAKKSEKKGEHNSEDGDSEDSILPEI-DLNTATPAELLDLVTAVLRKCVPGVYKVQPQKTTRLPLVRFIHRDLNLQGDITINNRLAVRNTRFLQLCSGLDSRVRPLVYTVRFWAKQKHLAGNPFGGGPLLNNYALTLLVLFYLQNVDPPILPTVEKLKSMACEEEECVIDGWDCTFPSQPISVPPSKNTDDLCT-----LLHGFFSFYS-KFDFSGSVVSLREGRSLPIASFLSRDEEMPDTADSSSSKHKKAAGPKLGPVNILDPFNLHHNVAGNLSERTHRHLLREC 512
BLAST of terminal uridylyltransferase 4 vs. Ensembl Sea Lamprey
Match: tut1 (terminal uridylyl transferase 1, U6 snRNA-specific [Source:NCBI gene;Acc:564388]) HSP 1 Score: 115.161 bits (287), Expect = 2.908e-26 Identity = 93/325 (28.62%), Postives = 159/325 (48.92%), Query Frame = 1
Query: 2464 EIVKALYPIARPQLFGSTINGFMTKASDLDVCLEF----YVDDKLQSLSNNEVSNR-----------------------LKTIGRELKK-LNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEI-GDASQGT--LSSYAYMLMLIHFLQKRG--ILPILQELYE--GERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEY---LWLDFFYYYAVEFDIDKEVVTIRQS-----KQLFKIDKNWQS--------AYMAVEDPFNLSHNLCGSLSLENA-RYILEC 3282
E+ +P FGST+NGF + SD+D+ L+ K Q L+ + S+ L+ + L+K + V ++ + A++P++KF + + +Q DI+INN L +QNTK+L++YA VK L ++Y AK ++ G+ S G L++YA L++++FLQ R ILP + L E GE T +DGWD + + W + + + N + L +FF +YA FD V+++R+ + F + + ++ + V+DPF L+HN+ G++S A R+ EC
Sbjct: 101 EVFTEFFPGCIIHPFGSTVNGFGLRGSDMDLFLDLENAKTFQAKSQKLTEGDESSDESVHSEESMHSEVDLEGALPAEVLEMVALVLRKCVPGVHKVQAVPTARLPVVKFIHRESGLQCDITINNRLPVQNTKLLQLYAAADSRVKPLVYTVRYWAKQKQLAGNTSGGGPHLNNYALTLLVVYFLQTRQPPILPTVSRLKELAGEVETHVVDGWDCSFLSE-------WTDECDTD-NTDTPNSLLPEFFKFYA-SFDFSGSVISLREGVVFPVAEFFGVSQTEEANAKPKLKLGPINVQDPFELNHNVAGNVSERMARRFKKEC 416 HSP 2 Score: 53.5286 bits (127), Expect = 3.911e-7 Identity = 35/106 (33.02%), Postives = 55/106 (51.89%), Query Frame = 1
Query: 739 IKFDKSCSGLFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFP---SEAFSILVIFYLQQLSIPILPNLHKLAELQSQ 1047
+KF SGL C I+ +NRL N++L++LY DSR + L RY+A + + G P + A ++LV+++LQ PILP + +L EL +
Sbjct: 208 VKFIHRESGLQCD--------ITINNRLPVQNTKLLQLYAAADSRVKPLVYTVRYWAKQKQLAGNTSGGGPHLNNYALTLLVVYFLQTRQPPILPTVSRLKELAGE 305
BLAST of terminal uridylyltransferase 4 vs. Ensembl Sea Lamprey
Match: mtpap (mitochondrial poly(A) polymerase [Source:ZFIN;Acc:ZDB-GENE-101208-3]) HSP 1 Score: 67.3958 bits (163), Expect = 1.452e-11 Identity = 59/253 (23.32%), Postives = 122/253 (48.22%), Query Frame = 1
Query: 2458 ITEIVKALYPIARPQLFGSTINGF----------MTKASDLDVCLE-----FYVDDKLQSLSNNE--VSNRLKTIGRELKKLN-TVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT-LSSYAYMLMLIHFLQKR--GILPILQELYEGERP--TLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTI 3147
+ ++ ++P + FGS+IN F + L+V + F +D +++ LS+ + L +G L + + IL+A+ P+++F + +++Q D + NN + L++T+ML +Y + V+ L ++ A+S + + G ++++A M++ FLQKR I+P L++L + P ++G D +N+ I N ++ ++ L ++FF +Y F K + I
Sbjct: 238 LQDMCATIFPGTLVRPFGSSINSFGHTGCDLDMLLDLDGVLNVLAQVVKNNFELDYQVKMLSSERSVTQSVLSVMGECLDYFSPGCVGVQKILQARCPLVRFSHQPSSLQCDFTANNRIALKSTEMLYIYGQVDERVRQLVFAVRSWARSHGLTSSISGAWITNFALTTMVLFFLQKRSPAIVPTLEQLRKLADPEDVHIVEGHDCTIVSNLEKITSSQNTET-----LDQLLVEFFNFYG-SFSFSKHSINI 484
BLAST of terminal uridylyltransferase 4 vs. Ensembl Yeast
Match: PAP2 (Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia [Source:SGD;Acc:S000005475]) HSP 1 Score: 78.1814 bits (191), Expect = 4.348e-15 Identity = 70/310 (22.58%), Postives = 124/310 (40.00%), Query Frame = 1
Query: 2407 IVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAY-------MAVEDPFNLSHNLC-GSLSLENARYILECFAHILKLHSN 3312
I P+ + +I + I E VK L+P A +FGS SD+D + L E N L ++ LKK N ++ + KA+VPIIKF ++ I D+S + G++ K+++ + D +P ++ L ++K + + + G L ++ + ++ FL + II + K N + L ++FF Y F D + ++ W + +A++DP + S+N+ GS N R I + FA L +N
Sbjct: 190 ISPSREEIEIRNQTISTIREAVKQLWPDADLHVFGSYSTDLYLPGSDIDCVV-------TSELGGKESRNNLYSLASHLKKKNLATEVEVVAKARVPIIKFVEPHSGIHIDVSFERTNGIEAAKLIREWLDDTPGLRELVLIVKQFLHARRLNNVHTGGLGGFSIICLVFSFL-----------------------------HMHPRIITNEIDPKDNLGV----LLIEFFELYGKNFGYDDVALGSSDGYPVYFPKSTWSAIQPIKNPFSLAIQDPGDESNNISRGSF---NIRDIKKAFAGAFDLLTN 456
BLAST of terminal uridylyltransferase 4 vs. Ensembl Yeast
Match: TRF5 (Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p [Source:SGD;Acc:S000005243]) HSP 1 Score: 69.707 bits (169), Expect = 2.285e-12 Identity = 70/318 (22.01%), Postives = 131/318 (41.19%), Query Frame = 1
Query: 2374 LRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSA-------YMAVEDPFNLSHNLC-GSLSLENARYILECFAHILKL 3303
L +E+ I P+ + K + DK+ VK L+ A +FGS SD+D + DK E N + + R LK R++ I+K +VPIIKF + + D+S + GL+ K+++ + SP ++ L +IK S + + G L + + ++ FL + P ++ +I +++ N+ L +DFF Y F D ++I + W++ +A++DP + ++N+ GS ++++ I + FA +L
Sbjct: 176 LTSEIKDFVHYISPSKNEIKCRNRTIDKLRRAVKELWSDADLHVFGSFATDLYLPGSDIDCVVNSRNRDK-------EDRNYIYELARHLKNKGLAIRMEVIVKTRVPIIKFIEPQSQLHIDVSFERTNGLEAAKLIREWLRDSPGLRELVLIIKQFLHSRRLNNVHTGGLGGFTVICLVYSFL---NMHPRIK-------------------SNDIDVLD-----------NLGVLLIDFFELYGKNFGYDDVAISISDGYASYIPKSCWRTLEPSRSKFSLAIQDPGDPNNNISRGSFNMKD---IKKAFAGAFEL 450
BLAST of terminal uridylyltransferase 4 vs. Ensembl Nematostella
Match: EDO43972 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RX54]) HSP 1 Score: 245.358 bits (625), Expect = 2.017e-71 Identity = 150/428 (35.05%), Postives = 234/428 (54.67%), Query Frame = 1
Query: 2206 LVYLFDR-ISIHNRKLVFTCGLCNNKGHRKEICN-------YAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVS--NRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYML----LEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
+VY FD I VFTC C GH KE C A+PR + + ++I A +++ + N+ + +++ + + ++ +YP A LFGS++NGF K SDLD+C+ L + ++V + + ++LK+ + ++ + I AKVPI+KFY+++ +GDIS+ N+L L+N++ML+ YADL V+ LG +K AK C+IGDAS+G+LSSYAY+LML+H+LQ + ++PILQEL+ G+ P I+GW+ WY ++ + K+W K+ ++ + LWL F YY FD + +VV R+SK+L K +K W A+EDPFNLSHNL ++ + A +I F + L Y L L P + N RV C + GH A+DC
Sbjct: 3 IVYKFDSSILTGGHAPVFTCCYCKKDGHIKEKCPDLKKPVIVALPRMTK---VYQNIIDAACIRVGETMGL-------NHMEGQFRQEVLRNLEDYIREVYPAACLYLFGSSVNGFGFKESDLDICMT------LDGKTKDDVDPIKVIHDLSKKLKQHSDIRNVLAITTAKVPIVKFYIRSVKREGDISLYNTLALENSRMLRTYADLDVRVRQLGFTLKIFAKVCDIGDASKGSLSSYAYILMLLHYLQTCQPPVIPILQELHNGQCPNNMIEGWNCWYYNDLPNLPKVWKSKNRESVGL--LWLGFLRYYTETFDWEHDVVCCRRSKRLTKFEKMWTKHEFAIEDPFNLSHNLGAGITRKMATFISLVFRRGRERFGTPIQLPLQLYDEYFFNVDYLTDGALAPCDRNCRV---CGRIGHLAKDC 409 HSP 2 Score: 72.4034 bits (176), Expect = 4.707e-13 Identity = 47/175 (26.86%), Postives = 79/175 (45.14%), Query Frame = 1
Query: 802 ISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
IS N L NS ++R Y +D R L + FA I G S A+ ++++ YLQ P++P L QEL + ++ Y N + + ++W N S+ LW+G L++Y F+ +V R ++ + K ++ AIEDPF++ N+
Sbjct: 175 ISLYNTLALENSRMLRTYADLDVRVRQLGFTLKIFAKVCDIGDASKGSLSSYAYILMLLHYLQTCQPPVIPIL--------QELHNGQCPNNMIEGWNCWYYNDLPNLPKVWK-SKNRESVGLLWLGFLRYYTETFDWEHDVVCCRRSKRLTKFEKMWTKHEFAIEDPFNLSHNL 340
BLAST of terminal uridylyltransferase 4 vs. Ensembl Nematostella
Match: EDO41975 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2S2]) HSP 1 Score: 60.077 bits (144), Expect = 8.400e-10 Identity = 51/187 (27.27%), Postives = 94/187 (50.27%), Query Frame = 1
Query: 2686 AKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNI---------NIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQL-FKIDKNWQSAYMAVEDP 3216
A VPI+ ++ +++GDIS N+ + NT +++ Y +L VK L +IK A+S I +A LS +A +L++IH+LQ P+L L + + + A + + L N+ + + +I L + FF YY+ F+ + V+++R + L +K W++ + +EDP
Sbjct: 7 AVVPIVILQDRSRDLEGDISYENTNTVFNTFLMRCYGELDSRVKPLVIVIKAWAQSARITNARDHKLSGFALVLLVIHYLQVGCSPPVLPSLQQDPQFHGFFSESSA-----LKVAEHLENEYTPPPVSLYSSRSSASIGELLVGFFRYYS-SFNWAR-VLSVRTAGFLPLPYNKKWRNPEIRIEDP 186 HSP 2 Score: 48.1358 bits (113), Expect = 7.992e-6 Identity = 51/181 (28.18%), Postives = 81/181 (44.75%), Query Frame = 1
Query: 802 ISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQ-QLSIPILPNLHKLAELQS--QELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPA-------NEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPV-LKSDRHIVNRRLAIEDPFD 1311
IS N N+ L+R Y ++DSR + L + + +A A I +D A +LVI YLQ S P+LP+L + + E S + V+ L N+ + PP + S+ EL +G ++Y FN W ++S+R + L ++ N + IEDP D
Sbjct: 25 ISYENTNTVFNTFLMRCYGELDSRVKPLVIVIKAWAQSARITNARDHKLSGFALVLLVIHYLQVGCSPPVLPSLQQDPQFHGFFSESSALKVAEHLENE---------------YTPPPVSLYSSRSSASIGELLVGFFRYY-SSFN-WARVLSVRTAGFLPLPYNKKWRNPEIRIEDPTD 188
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Match: ENSORLT00000016466.3 (pep primary_assembly:ASM223467v1:4:22253493:22270034:-1 gene:ENSORLG00000013135.3 transcript:ENSORLT00000016466.3 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 222.246 bits (565), Expect = 4.482e-61 Identity = 123/321 (38.32%), Postives = 190/321 (59.19%), Query Frame = 1
Query: 2503 LFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKR--GILPILQELYEGER-PTLYIDGWDAWYQTNIT-IINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYM----LLEPSVLRPPNGNMRVCFSCNKQGHFARDC 3441
LFGS+ NGF + SDLD+C+ D + L+ E+ ++ + + LKK ++ I I AKVPI+KF + + ++GDIS+ N+L NT+ML YA L P V+ LG +K AK C+IGDAS+G+LSSYAY+LM+++FLQ+R ++P+LQE+++G P +DGW+A++ +I + +L ++ N ++ LWL +Y EFD + V++IRQ K+L +K W S +A+EDPF+L+HNL +S + +I++ F + KL V Y +L L P N R C C K GH+ +DC
Sbjct: 7 LFGSSKNGFGFRDSDLDICMTLEGHDSAEKLNCKEI---IEGLAKVLKKHTGLRNILPITTAKVPIVKFEHRQSGLEGDISLYNTLAQHNTRMLATYAALDPRVQFLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVLQEIFDGTTVPQRMVDGWNAFFFDDIADLRQRLAGRQPNME-SVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLFGTPFYPLPGTEVDYFFDSKVLTDGELAP---NDRCCRICGKIGHYMKDC 320 HSP 2 Score: 82.8037 bits (203), Expect = 1.450e-15 Identity = 49/181 (27.07%), Postives = 93/181 (51.38%), Query Frame = 1
Query: 787 GVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQ-LWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNI 1326
G+ IS N L N+ ++ Y +D R + L + FA + I G S A+ ++V+++LQQ P++P L ++ +V + +V+ + + + ++Q L N S+ ELW+GLL+FY +F+ +++SIR + + ++ ++ +AIEDPFD+ N+
Sbjct: 78 GLEGDISLYNTLAQHNTRMLATYAALDPRVQFLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRQPPVIPVLQEI-------FDGTTVPQRMVDGWNAFFFDDIADLRQRLAGRQPNMESVGELWLGLLRFYTEEFDFKEHVISIRQRKRLTTFEKQWTSKCIAIEDPFDLNHNL 251
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Match: ENSORLT00000002130.2 (pep primary_assembly:ASM223467v1:9:5319511:5326523:-1 gene:ENSORLG00000001714.2 transcript:ENSORLT00000002130.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 178.333 bits (451), Expect = 4.373e-46 Identity = 150/509 (29.47%), Postives = 233/509 (45.78%), Query Frame = 1
Query: 1969 EETGDREFVLEDV----EEIDVLDNNNGNPKGDNLKTCIQITDSWKLPIAFKQIYVNIK-GSAVSSDNPDEYNESQVENL-QKQ-DLVYLFDRISI-HNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAEL--------NAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
E+ G R L+ V EEI +D P G+ L + + ++ + P + ++ +++ S P + + EN QKQ VY F R + + V C LC GH K+ +C + + ++L L L +E +K P+ I E I ++ V+ + AR QLFGS+ NGF + SDLD+C+ V + +++ + + ++++ R LKK ++ I I AKVPI+KFY N +++GDIS+ N+L L NT +L YA + VK+L ++K AK C+IGDAS+G+LSSYAY LM++ FL TL + W + + W + +I LWL +Y +FD + VV+IRQ +L K W S Y+ +E + N LSL +A + F I++ +P VL N R C C K GHF +DC
Sbjct: 125 EDVGHRRHHLDSVTTEDEEIFAVDE----PSGEELLSDEEGSE-YGTPASMEEDEEDVELAPGRPSTAPVQVKNERPENTHQKQVTFVYKFTRQAFTRGKSHVVVCSLCKRDGHLKK----------DCPEDFKKVELHPLPPLTSEFVRVLDRVCEQCYKDFAPDELELSIRELILQQLEAFVRCQFAGARLQLFGSSKNGFGFRQSDLDICM---VLEGKENIDDVDCIRIIESLARCLKKNPDLKNILPITTAKVPIVKFYHINTSLEGDISLYNTLALHNTHLLASYAAIDRRVKILCYVMKVFAKMCDIGDASRGSLSSYAYTLMVLFFLH-----------------TLKV----VWIFVLLLLQPSHWPQHGKNTESIGELWLGLLRFYTEDFDFKEHVVSIRQRARLTTFSKQWTSKYIVIEGDLLAAFN----LSLPHAMMVDMSF--IVQ--------------QEYFFDPEVLTEGQVAPNDRCCRICGKVGHFMKDC 574
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Match: tent2 (terminal nucleotidyltransferase 2 [Source:NCBI gene;Acc:101155937]) HSP 1 Score: 132.88 bits (333), Expect = 4.976e-32 Identity = 86/259 (33.20%), Postives = 150/259 (57.92%), Query Frame = 1
Query: 2455 KITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQ--KRGILPILQELY-EGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKI-DKNWQSAYMAVEDPF 3219
++ E +++++ +AR L GS++NG ++SD D+CL + K LS V +RL+ + R L + +++AKVPI+KF K ++++ D++INN++G++NT +L+ YA P V+ + ++K A +I DAS+GTLSSY +LM++H+LQ + +LP LQ + + P + ID I++ N+ S + L L F YYA EF DK+V+++R++ K + W+ ++ VE+PF
Sbjct: 181 RLQEDIQSIFSVARLYLTGSSMNGLGCRSSDADLCLVITGNKKPDPLS---VLSRLRKLFRTLSYVEGT----CLIRAKVPILKFKEKGSDLEFDLNINNTVGIRNTFLLRSYAHADPRVRPMILVVKKWACHHQINDASKGTLSSYTLVLMVLHYLQTVRDPVLPSLQRDHPDCFSPCMEIDMVPEGSTHVPPYISR--NQSS-----LGELLLGFLRYYASEFSWDKQVISVREATAFPKTYAQEWRKKFICVEEPF 425
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Match: ENSORLT00000037624.1 (pep primary_assembly:ASM223467v1:9:5319511:5322186:-1 gene:ENSORLG00000001714.2 transcript:ENSORLT00000037624.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 99.7525 bits (247), Expect = 3.707e-22 Identity = 73/258 (28.29%), Postives = 111/258 (43.02%), Query Frame = 1
Query: 2677 ILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGE-RPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPN--GNMRVCFSCNKQGHFARDC 3441
I AKVPI+KFY N +++GDIS+ N+L L NT +L YA + VK+L ++K AK +Y G+ +P + +DGW+ ++ ++ + W + +I LWL +Y +FD + VV+IRQ +L K W S Y+ +E + N LSL +A + F I++ +P VL N R C C K GHF +DC
Sbjct: 15 ITTAKVPIVKFYHINTSLEGDISLYNTLALHNTHLLASYAAIDRRVKILCYVMKVFAK------------------------------------IYFGKHKPEVLVDGWNVYFFDDLKTLPSHWPQHGKNTESIGELWLGLLRFYTEDFDFKEHVVSIRQRARLTTFSKQWTSKYIVIEGDLLAAFN----LSLPHAMMVDMSF--IVQ--------------QEYFFDPEVLTEGQVAPNDRCCRICGKVGHFMKDC 216
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Match: tut1 (terminal uridylyl transferase 1, U6 snRNA-specific [Source:NCBI gene;Acc:101173262]) HSP 1 Score: 96.2857 bits (238), Expect = 1.341e-19 Identity = 85/355 (23.94%), Postives = 161/355 (45.35%), Query Frame = 1
Query: 2353 DLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEF-------------------------------YVDDKLQSLSNNEVSNRLKTIGRELKK-LNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGT---LSSYAYMLMLIHFLQ--KRGILPILQELYE--GERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKID-----------------KNWQSAYMAVEDPFNLSHNLCGSL 3249
+L L + A++ + + + K E + + EI +P + FGS++N F + DLD+ L+ D L + S EV + + TI LK+ + V ++ + A++P++KF N+QGDI+INN LG++NT+ L++ + + ++ L I++ A+ ++ G L++YA L++I +LQ + +LP +++L + E I+GW+ + + + N + ++ L FF +YA +FD VV++R+ + L +D ++ + M + DPF LSHN+ G+L
Sbjct: 152 ELCQLPSVDAQMQYVVERLQLGENEKKARELLVQLLQEIFVEFFPDSEILPFGSSVNTFGIHSCDLDLFLDLENTKTFQARAKSTTEQVGEGVSDDGRSEDSILSDIDLTTASPAEVLDLVATI---LKRCVPNVHKVHVVGTARLPVVKFQHHKLNLQGDITINNRLGVRNTRFLQLCSGMEERLRPLVYTIRFWARQKKLAGNPSGAGPLLNNYALTLLVIFYLQNCEPPVLPTVEQLKDMACEEEECVIEGWNCTFPSQPIAVLPSKNTQ-----DLSSLLAGFFSFYA-KFDFASNVVSLREGRALPVVDFLGKGKEEEENPPKGSRQHPKLGSMTLLDPFELSHNVAGNL 497
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Match: SMESG000041562.1 (SMESG000041562.1) HSP 1 Score: 2670.96 bits (6922), Expect = 0.000e+0 Identity = 1340/1344 (99.70%), Postives = 1343/1344 (99.93%), Query Frame = 1
Query: 157 DKGSAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICXXXXXXXXXXXXXDVNLFLEIEPTNIKETGYLSAMEKFSVVFKNKLQLKVEIKFDKSCSGLFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKKVNFVEITLSNLKPIANVTPIFTKDNFTETINIIETDFKKFYTEQKSFYDVSKWPVELFVKACLLKILNSVFNSEIDIKNLVCLLELKTKGVAYFLIENYVKFKEYAIRLSKCVLQEFSNHFVINDSWAQPDFENRIAFELIKKSSQKFINESXXXXXXXXXXXXXXXXXXXXXXXXIEETGDREFVLEDVEEIDVLDNNNGNPKGDNLKTCIQITDSWKLPIAFKQIYVNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDCVDKGNKRTPTKAAPNQQPGLPSITAPLQFKPSSVASRVYLQLEVPESPDNETINPYNIPLKAFKADVNQFSVCPISMKFGENTVIVPMLVGKNEVIEKKLNTHLKNGLNNNVCIFPVENIKPIIDHTKHVEDAFINTPLNSAYRRQTPIKNEMTVANNLNKINQQPMHRAIVPHHNVPQRMNFDMPMRMLYPQNNMTRYPMHTSIPPGYHYQPEQLRYGSPNWNYHEAENRRMIRPPLNNNHTPRGNRK 4188
DKGSAVINKKKRKQENMLIDPSLLETL+STYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICGSLLYGLSLVSVSDVNLFLEIEPTNIKETGYLSAMEKFSV+FKNKLQLKVEIKFDKSCSGLFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSE+FSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKKVNFVEITLSNLKPIANVTPIFTKDNFTETINIIETDFKKFYTEQKSFYDVSKWPVELFVKACLLKILNSVFNSEIDIKNLVCLLELKTKGVAYFLIENYVKFKEYAIRLSKCVLQEFSNHFVINDSWAQPDFENRIAFELIKKSSQKFINESLNILHNDDEDDDELEDLPDSDNNEIEETGDREFVLEDVEEIDVLDNNNGNPKGDNLKTCIQITDSWKLPIAFKQIYVNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDCVDKGNKRTPTKA PNQQPGLPSITAPLQFKPSSVASRVYLQLEVPESPDNETINPYNIPLKAFKADVNQFSVCPISMKFGENTVIVPMLVGKNEVIEKKLNTHLKNGLNNNVCIFPVENIKPIIDHTKHVEDAFINTPLNSAYRRQTPIKNEMTVANNLNKINQQPMHRAIVPHHNVPQRMNFDMPMRMLYPQNNMTRYPMHTSIPPGYHYQPEQLRYGSPNWNYHEAENRRMIRPPLNNNHTPRGNRK
Sbjct: 4 DKGSAVINKKKRKQENMLIDPSLLETLNSTYISLLNKPSNKFPKAKFVCKICPYHMDDIDRAIKHAQDNIHKRNINKLCTKNLLTYLPNPTKIQLEAVNECLENTLKEPIFIGNEEYTRRIGLCMKMNELAREINPGYRLIICGSLLYGLSLVSVSDVNLFLEIEPTNIKETGYLSAMEKFSVIFKNKLQLKVEIKFDKSCSGLFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSESFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILFRWNSTDVVDFLNDQLQVCFIYFAVPQLRTGPAFKKVNFVEITLSNLKPIANVTPIFTKDNFTETINIIETDFKKFYTEQKSFYDVSKWPVELFVKACLLKILNSVFNSEIDIKNLVCLLELKTKGVAYFLIENYVKFKEYAIRLSKCVLQEFSNHFVINDSWAQPDFENRIAFELIKKSSQKFINESLNILHNDDEDDDELEDLPDSDNNEIEETGDREFVLEDVEEIDVLDNNNGNPKGDNLKTCIQITDSWKLPIAFKQIYVNIKGSAVSSDNPDEYNESQVENLQKQDLVYLFDRISIHNRKLVFTCGLCNNKGHRKEICNYAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNRPDNVHYMLLEPSVLRPPNGNMRVCFSCNKQGHFARDCVDKGNKRTPTKATPNQQPGLPSITAPLQFKPSSVASRVYLQLEVPESPDNETINPYNIPLKAFKADVNQFSVCPISMKFGENTVIVPMLVGKNEVIEKKLNTHLKNGLNNNVCIFPVENIKPIIDHTKHVEDAFINTPLNSAYRRQTPIKNEMTVANNLNKINQQPMHRAIVPHHNVPQRMNFDMPMRMLYPQNNMTRYPMHTSIPPGYHYQPEQLRYGSPNWNYHEAENRRMIRPPLNNNHTPRGNRK 1347
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Match: SMESG000007092.1 (SMESG000007092.1) HSP 1 Score: 183.341 bits (464), Expect = 2.286e-50 Identity = 114/341 (33.43%), Postives = 182/341 (53.37%), Query Frame = 1
Query: 2320 FDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQE--LYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCN---INIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLN 3327
+ + +D ++SI+ + KL L F S + + ++ I +K + LFGS I G K SDLD+ + FY + + L+N N+L TI +LN I+ I A+ PI+K K N + DIS +N++ + NT+++++Y+ L P +++LG ++K+V K EI D GTLSS+ Y LMLIHFLQKR +LPIL + Y R I + W+ + +N W CN ++I LW+ FF YY +EFD EV+ IRQ + L K K W M++EDP + ++N+ ++S + IL+ F +L N ++++
Sbjct: 23 YSQVKDSIDSINDDYKRKLWQVLYEFFNSNTMHPSEQESKKRELADIRSYIKQTFFNTDVYLFGSAITGLAHKGSDLDITVLFYHRNSVLDLTNKTQINKLATIENYFTQLNRFSEIEGIYSARRPILKVKTKTN-LSVDISASNTIAVDNTELIRLYSILDPRLEILGRMLKHVVKLYEICDTRTGTLSSFGYTLMLIHFLQKRNVLPILSDRPTYSKNRRPRPIYSCEMWHVPDSVKLNNAW----TCNSPGLSINELWIAFFKYYTMEFDFQTEVINIRQIQPLLKSTKMWTHIMMSIEDPTDTTNNVGYNISFLYYQKILDAFCEVLLAKGNINDIS 358 HSP 2 Score: 72.7886 bits (177), Expect = 4.702e-13 Identity = 50/190 (26.32%), Postives = 96/190 (50.53%), Query Frame = 1
Query: 766 LFCKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSI-PILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDIKRNILF 1332
L K K ++ ISASN + N+ELIRLY +D R E L ++ ++ I + G S +++++I +LQ+ ++ PIL + ++ + + ++ +V + W + +S+ ELWI K+Y ++F+ +++IR +P+LKS + + ++IEDP D N+ +
Sbjct: 150 LKVKTKTNLSVDISASNTIAVDNTELIRLYSILDPRLEILGRMLKHVVKLYEICDTRTGTLSSFGYTLMLIHFLQKRNVLPILSDRPTYSKNRRPRP---------IYSCEMWHVPDSVKLNNAWTCNSPGLSINELWIAFFKYYTMEFDFQTEVINIRQIQPLLKSTKMWTHIMMSIEDPTDTTNNVGY 330
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Match: SMESG000007097.1 (SMESG000007097.1) HSP 1 Score: 183.341 bits (464), Expect = 3.409e-50 Identity = 114/332 (34.34%), Postives = 185/332 (55.72%), Query Frame = 1
Query: 2341 LESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQEL--YEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSNSDNLNR 3330
++SID + KLR L + + + +K F I + + L+GS + G + SDLD+ + F+ ++ L+N LK I L +LN I+ + A++PII+ K N ++ DIS +N++ + NT++++ Y+D+ P V +LG L+K+V EIGDA GTLSS+ Y LMLIHFLQKR +LPIL + Y + + + W ++IT +N N +S +I LW+DFF YY +EFD + EVV+IRQ + L K KNW +A+EDP N+++ + ++ + + IL+ F +L + N + ++R
Sbjct: 28 IDSIDGTYKRKLRHILLEFISNNTISKSEQRSRKKEFYDIENCIDEKFYNVDVYLYGSHVTGLAHRNSDLDITVLFHNRKSIEDLNNITQKRMLKRIENYLLQLNKFTEIEGVYSARIPIIRCKTKKN-LRVDISASNTIAIDNTEIVREYSDMDPRVSILGRLLKHVVNLYEIGDARSGTLSSFGYTLMLIHFLQKRNVLPILPDRPKYSDNKRPRPKNNCEMWRYSDITNMN---NAQS-LERSITELWIDFFKYYTMEFDFEVEVVSIRQKQPLLKSTKNWIFKVIAIEDPSNITNAIGANIRQNDFQKILDAFCEVLLVRGNINEISR 354 HSP 2 Score: 80.8777 bits (198), Expect = 1.244e-15 Identity = 57/182 (31.32%), Postives = 95/182 (52.20%), Query Frame = 1
Query: 772 CKDKLGVTYHISASNRLQHLNSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSI-PILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFDI 1314
CK K + ISASN + N+E++R Y +D R L +L ++ + I + G S +++++I +LQ+ ++ PILP+ K SD R N Y + I + N + E S+ ELWI K+Y ++F+ +VSIR +P+LKS ++ + + +AIEDP +I
Sbjct: 150 CKTKKNLRVDISASNTIAIDNTEIVREYSDMDPRVSILGRLLKHVVNLYEIGDARSGTLSSFGYTLMLIHFLQKRNVLPILPDRPKY--------SDNKRPRPKNNCEMWRYSD----ITNMNNAQSLERSITELWIDFFKYYTMEFDFEVEVVSIRQKQPLLKSTKNWIFKVIAIEDPSNI 319
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Match: SMESG000007068.1 (SMESG000007068.1) HSP 1 Score: 156.762 bits (395), Expect = 2.323e-41 Identity = 111/336 (33.04%), Postives = 187/336 (55.65%), Query Frame = 1
Query: 2338 ILESIDLAWLVKLRAELNAKF-KSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGS-LSLENARYILECFAHILKLHSNSDNL--NRP 3333
+L SID W +LR L + K+ + N++ +I + +F++I I+ + A ++GS ++G K SDLD+ + F+ L +L + ++ ++ +I KA++P+IKF + + D+S++N +++T++L++Y+ LS +L L+K V K IG+A +GTLSSY YM+MLIHFLQK+ ILP+L E Y ++ NI I LWN + + LW+DFF YY +EF++ EVV+IR S+ + K +K W+S ++A+E+P + S + CGS L+ N I + F +L + + + NRP
Sbjct: 21 VLHSIDNEWKTELREVLKQFYHKNHITVNEQEEI-KALFNEIACIINDNFSNAIIYVYGSHVSGLAIKYSDLDIAVLFHDRKNFIYLPKGGQKFKLDMMYTYFSRMKKYLKLIFISKARIPLIKF-QTFDGVDVDVSVDNFQAMESTELLRIYSSLSSYFVVLAQLLKNVVKLYGIGEAYEGTLSSYGYMVMLIHFLQKKEILPVLHEKYNHYEIRPNFKACQNFFVPNINEIKNLWN-----DFLLVDLWIDFFRYYTLEFNVLYEVVSIRHSRPVTKCEKKWRS-FLAIENPLDPSSD-CGSHLNETNFNKIFDSFRDVLFAKGDVNRIFQNRP 347 HSP 2 Score: 60.077 bits (144), Expect = 6.247e-9 Identity = 48/160 (30.00%), Postives = 80/160 (50.00%), Query Frame = 1
Query: 832 NSELIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDGLFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLSYVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDPFD 1311
++EL+R+Y + S LA+L + I + +G S + +++I +LQ+ I LP LH+ + E+ R Q +V + IK LWN + L +LWI ++Y ++FN + +VSIR + PV K ++ LAIE+P D
Sbjct: 164 STELLRIYSSLSSYFVVLAQLLKNVVKLYGIGEAYEGTLSSYGYMVMLIHFLQKKEI--LPVLHE--KYNHYEI------RPNFKACQNFFVPNINEIKNLWN----DFLLVDLWIDFFRYYTLEFNVLYEVVSIRHSRPVTKCEKKW-RSFLAIENPLD 308
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Match: SMESG000007086.1 (SMESG000007086.1) HSP 1 Score: 145.591 bits (366), Expect = 2.150e-35 Identity = 106/340 (31.18%), Postives = 175/340 (51.47%), Query Frame = 1
Query: 2305 YAIPRFDECQDILESIDLAWLVKLRAELNAKFKSIVPNNDRPKIAEKIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDKLQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIGDASQGTLSSYAYMLMLIHFLQKRGILPIL--QELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYA--VEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLCGSLSLENARYILECFAHILKLHSN 3312
Y P+ +E + + SID + L+ L ++ + + KI ++ F +++ ++K ++GS GF + SDLD L + +L LS +LK + + I +L A VPI+KF MKNN I D+S ++ G+ NT + + Y++ P V +L L+K+V + I GTLSS +Y +M+IHFLQ++ IL IL L +G D W+ + INK+W K ++ +I LW++FF Y+ FD++ +VVTI Q L K KNW+S ++++E+P + +L L+ N I + F+ IL L +
Sbjct: 11 YFYPKIEEINEAVASIDENCIENLQTILCQVYRKFQVSEEEEKILKEQFYEVSNLLKQKMTNVNVYVYGSYATGFANRKSDLDTTLLSFSGKRLNQLSKRSQRKKLKQALIIFQAWKGISIIVNVLDATVPILKFKMKNN-IDADLSASSIDGIYNTALFRRYSESDPRVLILIQLLKHVTELYNISSRKDGTLSSLSYYIMVIHFLQQKHILKILPRSSLDQGLSK---FDEDQNWFYRDEEKINKIW-KSNSVPDSISSLWIEFFKYFTNRSTFDMENQVVTINQLPPLAKSTKNWRS-FLSIENPIQTNIDLGKDLTKPNFTLIKDAFSEILFLKGD 344 HSP 2 Score: 74.7146 bits (182), Expect = 3.988e-13 Identity = 87/304 (28.62%), Postives = 141/304 (46.38%), Query Frame = 1
Query: 2374 LRAELNAKFKSIVPNNDRPKIAE--KIFDKITEIVKALYPIARPQLFGSTINGFMTKASDLDVCLEFYVDDK--LQSLSNNEVSNRLKTIGRELKKLNTVQRIDYILKAKVPIIKFYMKNNNIQGDISINNSLGLQNTKMLKVYADLSPCVKLLGNLIKYVAKSCEIG--DASQGTLSSYAYMLMLIHFLQKRGILPILQELYEGERPTLYIDGWDAWYQTNITIINKLWNKKSNCNINIEYLWLDFFYYYAVEFDIDKEVVTIRQSKQLFKIDKNWQSAYMAVEDPFNLSHNLC---GSLSLE 3258
L+ L+ K++S + D KI E +F I+ +K Y LFGS + G + S L++ + F+ +D LQ++ NE S + K++N V I ++K + K ++ + +L+ Y+D + +L L++ A + +IG + + LSS AY +MLIHFLQK+ L +L E+ + D WY+ + N WN ++ +I LW+ FF YY EFD VV+IR + K +S + +EDP S NL G + LE
Sbjct: 377 LKEVLHQKYESKIV--DECKIDEFRTLFYGISNQIKQKYRHLDVCLFGSHVTGLGNRNSKLEINILFHDNDHKILQNI--NEKSKK--------KRMNKVNSYLQIYTNNNSVLKIFAK--------ELHEVFLMI---LLRRYSDFDSRLCILMILVEEFAVNNQIGCENDNSEMLSSLAYSIMLIHFLQKKHNLKVLSEIESANLISRNNFKCDMWYKPTEKVENA-WNCAASGK-SIAELWIGFFEYYTKEFDAKNLVVSIRHDHTVSK-SAIQKSHDLVIEDPIKPSINLAVNIGKVKLE 654 HSP 3 Score: 53.9138 bits (128), Expect = 1.035e-6 Identity = 52/158 (32.91%), Postives = 78/158 (49.37%), Query Frame = 1
Query: 841 LIRLYLKIDSRAENLAKLFRYFAHKAFIDQIQDG--LFPSEAFSILVIFYLQQLSIPILPNLHKLAELQSQELSDVSVSRVLVNDTQLS-YVNGMQWIKQLWNPPANEMSLAELWIGLLKFYLVDFNSWFYMVSIRCTEPVLKSDRHIVNRRLAIEDP 1305
L+R Y DSR L L FA I D + S A+SI++I +LQ+ NL L+E++S L +SR N+ + + + ++ WN A+ S+AELWIG ++Y +F++ +VSIR V KS + L IEDP
Sbjct: 493 LLRRYSDFDSRLCILMILVEEFAVNNQIGCENDNSEMLSSLAYSIMLIHFLQKKH-----NLKVLSEIESANL----ISR---NNFKCDMWYKPTEKVENAWNCAASGKSIAELWIGFFEYYTKEFDAKNLVVSIRHDHTVSKSAIQ-KSHDLVIEDP 637 The following BLAST results are available for this feature:
BLAST of terminal uridylyltransferase 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of terminal uridylyltransferase 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of terminal uridylyltransferase 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of terminal uridylyltransferase 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of terminal uridylyltransferase 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026950 ID=SMED30026950|Name=terminal uridylyltransferase 4|organism=Schmidtea mediterranea sexual|type=transcript|length=4318bpback to top protein sequence of SMED30026950-orf-1 >SMED30026950-orf-1 ID=SMED30026950-orf-1|Name=SMED30026950-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp EKCISNIFKYNIWFAFKCFQQLNNKTAKFPITYHLHKLQISLFTFGFLWEback to top protein sequence of SMED30026950-orf-2 >SMED30026950-orf-2 ID=SMED30026950-orf-2|Name=SMED30026950-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1348bp MSNDKGSAVINKKKRKQENMLIDPSLLETLSSTYISLLNKPSNKFPKAKFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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