SMED30026949
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026949 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14Note: Hover over icons to view figure legend
Alignments
SMED30026949 aligns in the following genomic locations:
Homology
BLAST of SMED30026949 vs. Planmine SMEST
Match: SMESG000038520.1 (SMESG000038520.1) HSP 1 Score: 478.404 bits (1230), Expect = 2.016e-166 Identity = 268/291 (92.10%), Postives = 274/291 (94.16%), Query Frame = 3 Query: 483 ENLKQISASNSKTIDLGFSSINSPNEINVWNCHKQSTNDFTDVNGKPITYDHNQHTHSPRQYTSLSEAARIXXXXXXXXGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKAKLKLSXXXXXXXXXXXXIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSAL 1355 +N +QISASNSKTIDLGFSSINS NEINVWN HK STN FTD+NGKPITYD NQHTHSPRQYTSLSEAARISHHS+SSS KNFLLSKKSAPIYQHF NDTNSSSNWQNEFDKADLKRNSNEL YQDFDQIG NAK+KSN DRDKYSEGGAGS QNSRSH DNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDDCK KLKLSQERN QLENENEIFILEMENS NSNEKLRSECKILQNETNQYLAQINQLQSA+ Sbjct: 50 KNRRQISASNSKTIDLGFSSINSLNEINVWNSHKHSTNHFTDLNGKPITYDQNQHTHSPRQYTSLSEAARISHHSNSSSEKNFLLSKKSAPIYQHFLNDTNSSSNWQNEFDKADLKRNSNELNYQDFDQIGINAKLKSNADRDKYSEGGAGSSQNSRSHGDNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDCKEKLKLSQERNSQLENENEIFILEMENSNNSNEKLRSECKILQNETNQYLAQINQLQSAI 340 HSP 2 Score: 86.2705 bits (212), Expect = 6.002e-18 Identity = 57/61 (93.44%), Postives = 60/61 (98.36%), Query Frame = 3 Query: 1179 AKLKLSXXXXXXXXXXXXIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 1361 AKLKLSQERNLQLENENEIFIL+M+NS NSNEKL+SECKILQNETNQYLAQINQLQSALKE Sbjct: 376 AKLKLSQERNLQLENENEIFILQMKNSNNSNEKLKSECKILQNETNQYLAQINQLQSALKE 436
BLAST of SMED30026949 vs. Planmine SMEST
Match: SMESG000047804.1 (SMESG000047804.1) HSP 1 Score: 484.567 bits (1246), Expect = 1.526e-163 Identity = 272/292 (93.15%), Postives = 278/292 (95.21%), Query Frame = 3 Query: 486 NLKQISASNSKTIDLGFSSINSPNEINVWNCHKQSTNDFTDVNGKPITYDHNQHTHSPRQYTSLSEAARIXXXXXXXXGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKAKLKLSXXXXXXXXXXXXIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 1361 NL QISASNSKT FSSINSPNEINVWN HK S N FTD+NGKPITYDHNQHTHSPRQYTSLSEAARISHHS+SSSGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSR H DNYFSTNSADIYGTMP+WNDDYQSRNRDLHSELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDD KAKLKLSQERNLQLENENEIFILEMENS NSNEKL+SECKILQN+TNQYLA+INQLQSALKE Sbjct: 51 NLIQISASNSKTTVPSFSSINSPNEINVWNSHKHSINYFTDLNGKPITYDHNQHTHSPRQYTSLSEAARISHHSNSSSGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSRFHGDNYFSTNSADIYGTMPEWNDDYQSRNRDLHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDWKAKLKLSQERNLQLENENEIFILEMENSNNSNEKLKSECKILQNQTNQYLARINQLQSALKE 342
BLAST of SMED30026949 vs. Planmine SMEST
Match: SMESG000038528.1 (SMESG000038528.1) HSP 1 Score: 438.343 bits (1126), Expect = 2.033e-145 Identity = 208/216 (96.30%), Postives = 209/216 (96.76%), Query Frame = 3 Query: 39 QISATNRRTRVPSLSLRNRLTESNASNSHNQSTTDLTNINFMPTRYNQNQQYTPLPEIQKNLLNPTFSPKDQKFRLSIDRNNSATWQNSDINSDRNPGRGTRQKQIGKDRAIIKSADHHSLNRNNESWLKESEKINGKSQRDFELLSFENLKQISASNSKTIDLGFSSINSPNEINVWNCHKQSTNDFTDVNGKPITYDHNQHTHSPRQYTSLSEA 686 QISATNRRTRVPSLSLRNRLTESNASNSHNQSTTDLTNINFMPTRYNQNQQYTPLPEIQKNLLNPTFSPKDQKFRLSIDRNNSATWQNSDINSDRNPGRGTRQKQIGKDRAIIKSADHHSLNRNNESWLKESEKINGKSQRDFELLSFENLKQISASNSKTIDLGFSSIN PNEINVWNCHKQSTNDFTDVNGKPITYD NQHTHSP QY+ E Sbjct: 44 QISATNRRTRVPSLSLRNRLTESNASNSHNQSTTDLTNINFMPTRYNQNQQYTPLPEIQKNLLNPTFSPKDQKFRLSIDRNNSATWQNSDINSDRNPGRGTRQKQIGKDRAIIKSADHHSLNRNNESWLKESEKINGKSQRDFELLSFENLKQISASNSKTIDLGFSSINFPNEINVWNCHKQSTNDFTDVNGKPITYDQNQHTHSPSQYSPTPET 259
BLAST of SMED30026949 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 342.813 bits (878), Expect = 1.240e-107 Identity = 211/292 (72.26%), Postives = 221/292 (75.68%), Query Frame = 3 Query: 486 NLKQISASNSKTIDLGFSSINSPNEINVWNCHKQSTNDFTDVNGKPITYDHNQHTHSPRQYTSLSEAARIXXXXXXXXGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKAKLKLSXXXXXXXXXXXXIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 1361 NL+QISASNSKTIDLGFSSINSPNEINVWN HK STN FTD+N KPI Y NQHTHSPRQY+ + EAARI + +NSSS G NAK+KSN DRDKYS+GGA SRQNSR H DNYFSTNSADIYGTMP+WN+DYQSRN D HSELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDD KAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE Sbjct: 40 NLRQISASNSKTIDLGFSSINSPNEINVWNSHKYSTNHFTDLNVKPIAYYQNQHTHSPRQYSPIPEAARI-------------------------SHHSNSSS--------------------------GRNAKLKSNADRDKYSDGGARSRQNSRFHGDNYFSTNSADIYGTMPEWNNDYQSRNSDWHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDWKAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 280
BLAST of SMED30026949 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1) HSP 1 Score: 342.813 bits (878), Expect = 5.376e-107 Identity = 211/292 (72.26%), Postives = 221/292 (75.68%), Query Frame = 3 Query: 486 NLKQISASNSKTIDLGFSSINSPNEINVWNCHKQSTNDFTDVNGKPITYDHNQHTHSPRQYTSLSEAARIXXXXXXXXGKNFLLSKKSAPIYQHFPNDTNSSSNWQNEFDKADLKRNSNELTYQDFDQIGSNAKIKSNIDRDKYSEGGAGSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHSELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKAKLKLSXXXXXXXXXXXXIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 1361 NL+QISASNSKTIDLGFSSINSPNEINVWN HK STN FTD+N KPI Y NQHTHSPRQY+ + EAARI + +NSSS G NAK+KSN DRDKYS+GGA SRQNSR H DNYFSTNSADIYGTMP+WN+DYQSRN D HSELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDD KAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE Sbjct: 40 NLRQISASNSKTIDLGFSSINSPNEINVWNSHKYSTNHFTDLNVKPIAYYQNQHTHSPRQYSPIPEAARI-------------------------SHHSNSSS--------------------------GRNAKLKSNADRDKYSDGGARSRQNSRFHGDNYFSTNSADIYGTMPEWNNDYQSRNSDWHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDWKAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKE 280 The following BLAST results are available for this feature:
BLAST of SMED30026949 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30026949 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30026949 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30026949 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30026949 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026949 ID=SMED30026949|Name=SMED30026949|organism=Schmidtea mediterranea sexual|type=transcript|length=1361bpback to top protein sequence of SMED30026949-orf-1 >SMED30026949-orf-1 ID=SMED30026949-orf-1|Name=SMED30026949-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=453bp SRSSSAHNRNRRQISATNRRTRVPSLSLRNRLTESNASNSHNQSTTDLTNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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