Myosin light chain protein

Overview
NameMyosin light chain protein
Smed IDSMED30026887
Length (bp)986
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Myosin light chain protein (SMED30026887) t-SNE clustered cells

Violin plots show distribution of expression levels for Myosin light chain protein (SMED30026887) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myosin light chain protein (SMED30026887) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myosin light chain protein (SMED30026887) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30026887

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30026887SMESG000075512.1 dd_Smed_v4_223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026887SMESG000075512.1 Contig54050newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026887SMESG000075512.1 Contig54050uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
musculature systemSMED30026887SMESG000075512.1 dd_Smed_v6_223_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle progenitor cellSMED30026887SMESG000075512.1 dd_Smed_v6_223_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30026887SMESG000075512.1 dd_Smed_v6_223_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Myosin light chain protein vs. Ensembl Human
Match: MYL6B (myosin light chain 6B [Source:HGNC Symbol;Acc:HGNC:29823])

HSP 1 Score: 110.538 bits (275), Expect = 2.574e-28
Identity = 56/150 (37.33%), Postives = 94/150 (62.67%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            +K ++E+ +E F+LFD     DG +   + GD++R  G NPTN   +K   +  ++++  ++  FE F+P+ + + + +  GT+ DY+E F+ FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F+K +L
Sbjct:   62 NKDQLEEFKEAFELFDRVG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLA---GHEDSNGCINYEAFLKHIL 206          
BLAST of Myosin light chain protein vs. Ensembl Human
Match: MYL6B (myosin light chain 6B [Source:HGNC Symbol;Acc:HGNC:29823])

HSP 1 Score: 110.538 bits (275), Expect = 2.574e-28
Identity = 56/150 (37.33%), Postives = 94/150 (62.67%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            +K ++E+ +E F+LFD     DG +   + GD++R  G NPTN   +K   +  ++++  ++  FE F+P+ + + + +  GT+ DY+E F+ FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F+K +L
Sbjct:   62 NKDQLEEFKEAFELFDRVG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLA---GHEDSNGCINYEAFLKHIL 206          
BLAST of Myosin light chain protein vs. Ensembl Human
Match: MYL6 (myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:7587])

HSP 1 Score: 106.301 bits (264), Expect = 2.231e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   ++ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M +K   FE F+P+ + + + K+ GT+ DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE F++ +L G
Sbjct:    1 MCDFTEDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEAFVRHILSG 151          
BLAST of Myosin light chain protein vs. Ensembl Human
Match: MYL6 (myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:7587])

HSP 1 Score: 106.301 bits (264), Expect = 2.231e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   ++ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M +K   FE F+P+ + + + K+ GT+ DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE F++ +L G
Sbjct:    1 MCDFTEDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEAFVRHILSG 151          
BLAST of Myosin light chain protein vs. Ensembl Human
Match: MYL6 (myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:7587])

HSP 1 Score: 105.145 bits (261), Expect = 5.609e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   ++ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M +K   FE F+P+ + + + K+ GT+ DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE+ ++ VL G
Sbjct:    1 MCDFTEDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEELVRMVLNG 151          
BLAST of Myosin light chain protein vs. Ensembl Celegans
Match: mlc-3 (Myosin, essential light chain [Source:UniProtKB/Swiss-Prot;Acc:P53014])

HSP 1 Score: 127.872 bits (320), Expect = 5.366e-36
Identity = 67/145 (46.21%), Postives = 95/145 (65.52%), Query Frame = 1
Query:  322 IREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            ++E+F+L+D  +  DG +D  +VGD+ R  G+ PT  +  K    E  + G K+  FEE++P+YE++ +EKE GT+AD+ E  K FD+E  G I  AE+RH+L   GE+LS +E D +LK     ED +G VKYEDFIKKVL GP
Sbjct:    9 LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLKGV---EDGEGMVKYEDFIKKVLAGP 148          
BLAST of Myosin light chain protein vs. Ensembl Celegans
Match: mlc-3 (Myosin, essential light chain [Source:UniProtKB/Swiss-Prot;Acc:P53014])

HSP 1 Score: 127.872 bits (320), Expect = 5.366e-36
Identity = 67/145 (46.21%), Postives = 95/145 (65.52%), Query Frame = 1
Query:  322 IREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            ++E+F+L+D  +  DG +D  +VGD+ R  G+ PT  +  K    E  + G K+  FEE++P+YE++ +EKE GT+AD+ E  K FD+E  G I  AE+RH+L   GE+LS +E D +LK     ED +G VKYEDFIKKVL GP
Sbjct:    9 LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLKGV---EDGEGMVKYEDFIKKVLAGP 148          
BLAST of Myosin light chain protein vs. Ensembl Celegans
Match: mlc-5 (Myosin-2 essential light chain [Source:UniProtKB/Swiss-Prot;Acc:Q9XVI9])

HSP 1 Score: 99.3673 bits (246), Expect = 2.465e-25
Identity = 55/147 (37.41%), Postives = 86/147 (58.50%), Query Frame = 1
Query:  310 EIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            ++ D REVF  FD     D  +   +VGD+LR  G NPT      C+  G+ ++    +  FE+FVPI++ + + +E  T  +++E    FD+EG G I++AE+RH+LT  GE+LS+E+ D +L     H D  GNV   DF++ V+
Sbjct:    3 DLADCREVFAYFD--SKGDERISVQQVGDVLRALGQNPTEAEIHKCV--GSFDREA--RLSFEDFVPIFQSVSKNREKHTVEEFVEGLSHFDKEGNGMINVAELRHLLTTLGERLSDEDVDQLLS---GHNDSHGNVNISDFVRAVM 140          
BLAST of Myosin light chain protein vs. Ensembl Celegans
Match: mlc-5 (Myosin-2 essential light chain [Source:UniProtKB/Swiss-Prot;Acc:Q9XVI9])

HSP 1 Score: 99.3673 bits (246), Expect = 2.465e-25
Identity = 55/147 (37.41%), Postives = 86/147 (58.50%), Query Frame = 1
Query:  310 EIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            ++ D REVF  FD     D  +   +VGD+LR  G NPT      C+  G+ ++    +  FE+FVPI++ + + +E  T  +++E    FD+EG G I++AE+RH+LT  GE+LS+E+ D +L     H D  GNV   DF++ V+
Sbjct:    3 DLADCREVFAYFD--SKGDERISVQQVGDVLRALGQNPTEAEIHKCV--GSFDREA--RLSFEDFVPIFQSVSKNREKHTVEEFVEGLSHFDKEGNGMINVAELRHLLTTLGERLSDEDVDQLLS---GHNDSHGNVNISDFVRAVM 140          
BLAST of Myosin light chain protein vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 83.1889 bits (204), Expect = 3.080e-19
Identity = 53/145 (36.55%), Postives = 85/145 (58.62%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN-RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFI 729
            L++ +I + +E F LFD  DG DG +   ++G ++R  G NPT   +       +  G     F EF+ +  + +++ +S    +  EAF+ FD++G GFIS AE+RHV+T  GEKL++EE D +++  D   D DG V YE+F+
Sbjct:    5 LTEEQIAEFKEAFSLFD-KDG-DGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSE--EEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADI--DGDGQVNYEEFV 143          
BLAST of Myosin light chain protein vs. Ensembl Fly
Match: Mlc-c (gene:FBgn0004687 transcript:FBtr0070783)

HSP 1 Score: 102.449 bits (254), Expect = 2.559e-26
Identity = 55/153 (35.95%), Postives = 91/153 (59.48%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M A ++ ++ + +E F+LFD  +  DG +   +VG+ LR  G NPT    +K    +    ++  FE F+PIY+ I + +   T  D++E  + FD++  G+IS AE+RH+LT  GEKL++EE + +L      ED  GN+ YE+F++ V+ G
Sbjct:    1 MAAYTEDQLAEFQEAFNLFD--NRGDGKIQLSQVGECLRALGQNPTESD-VKKCTHQLKPDERISFEVFLPIYQAISKARSGDTADDFIEGLRHFDKDASGYISSAELRHLLTTLGEKLTDEEVEQLLA---NMEDQQGNINYEEFVRMVMSG 147          
BLAST of Myosin light chain protein vs. Ensembl Fly
Match: Mlc1 (gene:FBgn0002772 transcript:FBtr0344131)

HSP 1 Score: 100.908 bits (250), Expect = 1.314e-25
Identity = 53/155 (34.19%), Postives = 90/155 (58.06%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGM-VDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            M  + K E+E++  VF++     G  G  +DA+ +GD LR   +NPT  +  K G T+K   K+ K +EF+PIY ++ +EKE G + D++E  K +D+E  G + LAE++H L   GE L +E+ + +       ED +G + Y  F+++++  P
Sbjct:    1 MADVPKREVENVEFVFEVM----GSPGEGIDAVDLGDALRALNLNPTLALIEKLGGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMLLAELQHALLALGESLDDEQVETLFADCMDPEDDEGFIPYSQFVQRLMSDP 151          
BLAST of Myosin light chain protein vs. Ensembl Fly
Match: Mlc1 (gene:FBgn0002772 transcript:FBtr0085196)

HSP 1 Score: 100.908 bits (250), Expect = 1.314e-25
Identity = 53/155 (34.19%), Postives = 90/155 (58.06%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGM-VDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            M  + K E+E++  VF++     G  G  +DA+ +GD LR   +NPT  +  K G T+K   K+ K +EF+PIY ++ +EKE G + D++E  K +D+E  G + LAE++H L   GE L +E+ + +       ED +G + Y  F+++++  P
Sbjct:    1 MADVPKREVENVEFVFEVM----GSPGEGIDAVDLGDALRALNLNPTLALIEKLGGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMLLAELQHALLALGESLDDEQVETLFADCMDPEDDEGFIPYSQFVQRLMSDP 151          
BLAST of Myosin light chain protein vs. Ensembl Fly
Match: Mlc-c (gene:FBgn0004687 transcript:FBtr0308579)

HSP 1 Score: 99.7525 bits (247), Expect = 3.420e-25
Identity = 54/147 (36.73%), Postives = 88/147 (59.86%), Query Frame = 1
Query:  307 SEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            + +E+ +E F+LFD  +  DG +   +VG+ LR  G NPT    +K    +    ++  FE F+PIY+ I + +   T  D++E  + FD++  G+IS AE+RH+LT  GEKL++EE + +L      ED  GN+ YE+F++ V+ G
Sbjct:   13 TTLEEFQEAFNLFD--NRGDGKIQLSQVGECLRALGQNPTESD-VKKCTHQLKPDERISFEVFLPIYQAISKARSGDTADDFIEGLRHFDKDASGYISSAELRHLLTTLGEKLTDEEVEQLLA---NMEDQQGNINYEEFVRMVMSG 153          
BLAST of Myosin light chain protein vs. Ensembl Fly
Match: Mlc1 (gene:FBgn0002772 transcript:FBtr0085195)

HSP 1 Score: 98.5969 bits (244), Expect = 7.791e-25
Identity = 53/155 (34.19%), Postives = 89/155 (57.42%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGM-VDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            M  + K E+E++  VF++     G  G  +DA+ +GD LR   +NPT  +  K G T+K   K+ K +EF+PIY ++ +EKE G + D++E  K +D+E  G + LAE++H L   GE L +E+ + +       ED +G + Y  F+ ++ + P
Sbjct:    1 MADVPKREVENVEFVFEVM----GSPGEGIDAVDLGDALRALNLNPTLALIEKLGGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMLLAELQHALLALGESLDDEQVETLFADCMDPEDDEGFIPYSPFLARMCDRP 151          
BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Match: zgc:153867 (zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170])

HSP 1 Score: 110.923 bits (276), Expect = 2.174e-29
Identity = 59/156 (37.82%), Postives = 93/156 (59.62%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+ + I + K+ GTF D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F++ ++ G
Sbjct:    1 MSDFSEDQILEFKEAFLLFDRTG--DGKITYNQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNHKLLDFEQFLPMLQAIAKNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRHIMSG 151          
BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Match: zgc:153867 (zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170])

HSP 1 Score: 110.153 bits (274), Expect = 4.351e-29
Identity = 59/156 (37.82%), Postives = 93/156 (59.62%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+ + I + K+ GTF D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:    1 MSDFSEDQILEFKEAFLLFDRTG--DGKITYNQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNHKLLDFEQFLPMLQAIAKNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEELVRMVMSG 151          
BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Match: myl6 (myosin, light chain 6, alkali, smooth muscle and non-muscle [Source:ZFIN;Acc:ZDB-GENE-041010-28])

HSP 1 Score: 110.153 bits (274), Expect = 4.444e-29
Identity = 57/156 (36.54%), Postives = 94/156 (60.26%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  ++ +I + +E F LFD     DG +   + GD++R  G NP N   +K   + + E M +K   FE+F+P+ + I + K+ G+F D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F++ ++ G
Sbjct:    1 MSDFTEDQICEFKEAFLLFDRTG--DGKIMYNQCGDVMRALGQNPVNAEVLKVLGNPSNEDMNMKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRHIMAG 151          
BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Match: myl6 (myosin, light chain 6, alkali, smooth muscle and non-muscle [Source:ZFIN;Acc:ZDB-GENE-041010-28])

HSP 1 Score: 109.768 bits (273), Expect = 6.156e-29
Identity = 57/156 (36.54%), Postives = 94/156 (60.26%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  ++ +I + +E F LFD     DG +   + GD++R  G NP N   +K   + + E M +K   FE+F+P+ + I + K+ G+F D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:    1 MSDFTEDQICEFKEAFLLFDRTG--DGKIMYNQCGDVMRALGQNPVNAEVLKVLGNPSNEDMNMKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEELVRMVMSG 151          
BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Match: CR381567.1 (pep chromosome:GRCz11:CHR_ALT_CTG23_1_29:28397866:28403409:1 gene:ENSDARG00000111021.1 transcript:ENSDART00000192911.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR381567.1)

HSP 1 Score: 108.997 bits (271), Expect = 1.410e-28
Identity = 58/152 (38.16%), Postives = 91/152 (59.87%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+ + I + K+ GTF D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F++ ++ G
Sbjct:   11 SEDQILEFKEAFLLFDRTG--DGKITYNQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNHKLLDFEQFLPMLQAIAKNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRHIMSG 157          
BLAST of Myosin light chain protein vs. Ensembl Xenopus
Match: myl1 (myosin light chain 1 [Source:NCBI gene;Acc:394551])

HSP 1 Score: 107.842 bits (268), Expect = 1.448e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  283 SAMTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            S++ + S  E+ D +E F LFD     D  +   +V D++R  G NPTN   +  + + + E+M  K+ +FE+F+P+ + +   K+ G+F D++E  + FD+EG G +  AE+RHVL   GEK+ EEE + +L      ED +G + YE F+K ++
Sbjct:   37 SSVKSFSADEVADFKEAFLLFDRTG--DSKIALNQVADVMRALGQNPTNAEVKKVLGNPSAEEMNAKKIEFEQFLPMMQAVANNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMREEEVEALLA---GQEDSNGCINYEAFVKHIM 187          
BLAST of Myosin light chain protein vs. Ensembl Xenopus
Match: fa2h (fatty acid 2-hydroxylase [Source:Xenbase;Acc:XB-GENE-1014400])

HSP 1 Score: 107.071 bits (266), Expect = 2.667e-27
Identity = 57/150 (38.00%), Postives = 88/150 (58.67%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            S  +IE+ +E F LFD     +  +   + GD+LR  G NPTN   +K       E++ LK   F+ F+P+ + I + KE GT+ D++E  + FD+EG G +  AE+RHVL   GE+++EEE D +L+     ED +G++ YE F K +L
Sbjct:   40 SADQIEEFKEAFSLFDRTPKCEQKITYGQCGDVLRALGQNPTNAEVLKVLGKPKAEELSLKLMDFDTFLPMLQHISKSKEKGTYEDFVEGLRVFDKEGNGTVMGAEIRHVLATLGERMTEEEVDRLLQ---GQEDPNGHINYEAFAKYIL 186          
BLAST of Myosin light chain protein vs. Ensembl Xenopus
Match: myl1 (myosin light chain 1 [Source:NCBI gene;Acc:394551])

HSP 1 Score: 105.531 bits (262), Expect = 3.323e-27
Identity = 54/152 (35.53%), Postives = 89/152 (58.55%), Query Frame = 1
Query:  295 ALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            + S  E+ D +E F LFD     D  +   +V D++R  G NPTN   +  + + + E+M  K+ +FE+F+P+ + +   K+ G+F D++E  + FD+EG G +  AE+RHVL   GEK+ EEE + +L      ED +G + YE F+K ++
Sbjct:    2 SFSADEVADFKEAFLLFDRTG--DSKIALNQVADVMRALGQNPTNAEVKKVLGNPSAEEMNAKKIEFEQFLPMMQAVANNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMREEEVEALLA---GQEDSNGCINYEAFVKHIM 148          
BLAST of Myosin light chain protein vs. Ensembl Xenopus
Match: myl1 (myosin light chain 1 [Source:NCBI gene;Acc:394551])

HSP 1 Score: 105.531 bits (262), Expect = 3.323e-27
Identity = 54/152 (35.53%), Postives = 89/152 (58.55%), Query Frame = 1
Query:  295 ALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            + S  E+ D +E F LFD     D  +   +V D++R  G NPTN   +  + + + E+M  K+ +FE+F+P+ + +   K+ G+F D++E  + FD+EG G +  AE+RHVL   GEK+ EEE + +L      ED +G + YE F+K ++
Sbjct:    2 SFSADEVADFKEAFLLFDRTG--DSKIALNQVADVMRALGQNPTNAEVKKVLGNPSAEEMNAKKIEFEQFLPMMQAVANNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMREEEVEALLA---GQEDSNGCINYEAFVKHIM 148          
BLAST of Myosin light chain protein vs. Ensembl Xenopus
Match: abat (4-aminobutyrate aminotransferase [Source:Xenbase;Acc:XB-GENE-957494])

HSP 1 Score: 104.76 bits (260), Expect = 1.992e-26
Identity = 58/158 (36.71%), Postives = 85/158 (53.80%), Query Frame = 1
Query:  283 SAMTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S     S  +IE+ +E F LFD     +  +   + GD+LR  G NPTN   +K       E+M  K   FE F+P+ + I + K+ G+F D++E  + FD+E  G +  AE+RHVL   GEKL E E + +L      ED +G + YE FIK ++ G
Sbjct:   36 SVTVEFSADQIEEFKEAFQLFDRTPKGELKITYGQCGDVLRALGQNPTNAEVMKVLGRPKPEEMNTKLLDFETFLPMLQHISKNKDQGSFEDFVEGLRVFDKESNGTVMGAELRHVLATLGEKLQENEVEQLLA---GQEDANGCINYEAFIKHIMSG 190          
BLAST of Myosin light chain protein vs. Ensembl Mouse
Match: Myl6b (myosin, light polypeptide 6B [Source:MGI Symbol;Acc:MGI:1917789])

HSP 1 Score: 113.235 bits (282), Expect = 1.557e-29
Identity = 58/150 (38.67%), Postives = 93/150 (62.00%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            +K ++E+ RE F+LFD     DG +   + GDL+R  G NPTN   +K   +   E++  ++  FE F+P+ + + + ++ GT+ DY+E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F+K +L
Sbjct:   61 NKDQLEEFREAFELFDRVG--DGKILYSQCGDLMRALGQNPTNAEVLKVLGNPKNEELKSRRVDFETFLPMLQAVAKNRDQGTYEDYLEGLRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLA---GHEDSNGCINYEAFLKHIL 205          
BLAST of Myosin light chain protein vs. Ensembl Mouse
Match: Myl6 (myosin, light polypeptide 6, alkali, smooth muscle and non-muscle [Source:MGI Symbol;Acc:MGI:109318])

HSP 1 Score: 106.301 bits (264), Expect = 1.532e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   ++ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M +K   FE F+P+ + + + K+ GT+ DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE F++ +L G
Sbjct:    1 MCDFTEDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEAFVRHILSG 151          
BLAST of Myosin light chain protein vs. Ensembl Mouse
Match: Myl6 (myosin, light polypeptide 6, alkali, smooth muscle and non-muscle [Source:MGI Symbol;Acc:MGI:109318])

HSP 1 Score: 105.145 bits (261), Expect = 3.851e-27
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   ++ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M +K   FE F+P+ + + + K+ GT+ DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE+ ++ VL G
Sbjct:    1 MCDFTEDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEELVRMVLNG 151          
BLAST of Myosin light chain protein vs. Ensembl Mouse
Match: Myl4 (myosin, light polypeptide 4 [Source:MGI Symbol;Acc:MGI:97267])

HSP 1 Score: 106.301 bits (264), Expect = 4.812e-27
Identity = 57/152 (37.50%), Postives = 85/152 (55.92%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S  +IE+ +E F LFD     +  +   + GD+LR  G NPTN   ++       E+M  K   FE F+PI + I + KE GT+ D++E  + FD+E  G +  AE+RHVL   GEK+SE E + +L      ED +G + YE F+K ++ G
Sbjct:   45 SADQIEEFKEAFSLFDRTPTGEMKITYGQCGDVLRALGQNPTNAEVLRVLGKPKPEEMSSKTLDFEMFLPILQHISRNKEQGTYEDFVEGLRVFDKESNGTVMGAELRHVLATLGEKMSEAEVEQLL---SGQEDANGCINYEAFVKHIMSG 193          
BLAST of Myosin light chain protein vs. Ensembl Mouse
Match: Myl4 (myosin, light polypeptide 4 [Source:MGI Symbol;Acc:MGI:97267])

HSP 1 Score: 106.301 bits (264), Expect = 4.812e-27
Identity = 57/152 (37.50%), Postives = 85/152 (55.92%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S  +IE+ +E F LFD     +  +   + GD+LR  G NPTN   ++       E+M  K   FE F+PI + I + KE GT+ D++E  + FD+E  G +  AE+RHVL   GEK+SE E + +L      ED +G + YE F+K ++ G
Sbjct:   45 SADQIEEFKEAFSLFDRTPTGEMKITYGQCGDVLRALGQNPTNAEVLRVLGKPKPEEMSSKTLDFEMFLPILQHISRNKEQGTYEDFVEGLRVFDKESNGTVMGAELRHVLATLGEKMSEAEVEQLL---SGQEDANGCINYEAFVKHIMSG 193          
BLAST of Myosin light chain protein vs. UniProt/SwissProt
Match: sp|P07291|MLE_ARGIR (Myosin essential light chain, striated adductor muscle OS=Argopecten irradians OX=31199 PE=1 SV=2)

HSP 1 Score: 204.527 bits (519), Expect = 1.228e-64
Identity = 98/154 (63.64%), Postives = 120/154 (77.92%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            M  LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N      G T KMG K   FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT  GE+LS+E+ D I+K TD  ED++GNVKYEDF+KKV+ GP
Sbjct:    1 MPKLSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNEDVFAVGGTHKMGEKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLSDEDVDEIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153          
BLAST of Myosin light chain protein vs. UniProt/SwissProt
Match: sp|P05945|MLE_TODPA (Myosin catalytic light chain LC-1, mantle muscle OS=Todarodes pacificus OX=6637 PE=1 SV=2)

HSP 1 Score: 200.675 bits (509), Expect = 4.129e-63
Identity = 98/154 (63.64%), Postives = 122/154 (79.22%), Query Frame = 1
Query:  292 TALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFAD-YMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            + L+K EIE++REVFDLFDFWDGRDG VDA KVGDLLRC GMNPT     +HG T+KMG K YK EE +PIYE+ +  K++GT AD +MEAFKTFDREGQG IS AE+R+VL M GE+++E++ ++I  F D  EDIDGN+KYED +KKV+ GP
Sbjct:    2 SQLTKDEIEEVREVFDLFDFWDGRDGDVDAAKVGDLLRCLGMNPTEAQVHQHGGTKKMGEKAYKLEEILPIYEE-MSSKDTGTAADEFMEAFKTFDREGQGLISSAEIRNVLKMLGERITEDQCNDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154          
BLAST of Myosin light chain protein vs. UniProt/SwissProt
Match: sp|P07290|MLE_MIZYE (Myosin, essential light chain, adductor muscle OS=Mizuhopecten yessoensis OX=6573 PE=1 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 7.579e-63
Identity = 96/151 (63.58%), Postives = 118/151 (78.15%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N      G T KMG K   FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT  GE+L++EE D I+  TD  ED++GNVKYEDF+KKV+ GP
Sbjct:    3 LSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNDDVFAVGGTHKMGEKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLTDEEIDEIISLTDLQEDLEGNVKYEDFVKKVMAGP 152          
BLAST of Myosin light chain protein vs. UniProt/SwissProt
Match: sp|P53014|MLE_CAEEL (Myosin, essential light chain OS=Caenorhabditis elegans OX=6239 GN=mlc-3 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 6.826e-35
Identity = 67/145 (46.21%), Postives = 95/145 (65.52%), Query Frame = 1
Query:  322 IREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 750
            ++E+F+L+D  +  DG +D  +VGD+ R  G+ PT  +  K    E  + G K+  FEE++P+YE++ +EKE GT+AD+ E  K FD+E  G I  AE+RH+L   GE+LS +E D +LK     ED +G VKYEDFIKKVL GP
Sbjct:    9 LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLKGV---EDGEGMVKYEDFIKKVLAGP 148          
BLAST of Myosin light chain protein vs. UniProt/SwissProt
Match: sp|P02607|MYL6_CHICK (Myosin light polypeptide 6 OS=Gallus gallus OX=9031 GN=MYL6 PE=1 SV=3)

HSP 1 Score: 111.694 bits (278), Expect = 7.373e-29
Identity = 59/156 (37.82%), Postives = 93/156 (59.62%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M   S+ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  +++M LK  KFE+F+P+ + I + K+ G F DY+E  + FD+EG G +  AE+RHVL   GEK++EEE + ++     HED +G + YE+ ++ VL G
Sbjct:    1 MCDFSEEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEQLVA---GHEDSNGCINYEELVRMVLSG 151          
BLAST of Myosin light chain protein vs. TrEMBL
Match: E5Q336 (Myosin light chain protein OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 3.105e-106
Identity = 154/157 (98.09%), Postives = 155/157 (98.73%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 759
            MTALSKSEIEDIREVFDLFDFWDGRDGMVDA+KVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFI LAEMRHVLTMYGEKLSEEETDNILKFTD HEDIDGNVKYEDFIKKVLEGPKTK
Sbjct:    1 MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFIFLAEMRHVLTMYGEKLSEEETDNILKFTDIHEDIDGNVKYEDFIKKVLEGPKTK 157          
BLAST of Myosin light chain protein vs. TrEMBL
Match: A0A074ZUM9 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_02867 PE=4 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 4.265e-81
Identity = 116/164 (70.73%), Postives = 138/164 (84.15%), Query Frame = 1
Query:  268 KNRSNSAMTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 759
            + RS+   ++LSK+E+EDIREVFDLFDFWDGRDGM+DA+KVGDLLRC+GMNPT  IC+KHGAT K G KQYKF+EF+P YE I++E E GTFADYMEAFKTFDREGQGFIS AEMRHVLT YGE L + + D I+K  D HED+DGN+KYE+ IKKV++GP  K
Sbjct:  137 RGRSDKLCSSLSKAEVEDIREVFDLFDFWDGRDGMIDAVKVGDLLRCSGMNPTIAICVKHGATRKQGEKQYKFDEFLPCYEAILKENEGGTFADYMEAFKTFDREGQGFISAAEMRHVLTGYGEYLEDSDVDKIVKLIDLHEDLDGNIKYEELIKKVMQGPSAK 300          
BLAST of Myosin light chain protein vs. TrEMBL
Match: A0A5J4NZX3 (Uncharacterized protein (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0009174 PE=4 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 8.256e-81
Identity = 115/157 (73.25%), Postives = 138/157 (87.90%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 759
            M+ LSK+E+EDIREVFDLFDFWDGRDGM+DA+KVGDLLRC+G+NPT  + +KHGAT K G KQYKF+EF+P YE I++EKE+GTFADYMEA+KTFDREGQGFIS AEMRHVLT YGE+L ++E D I+KF D HED+DGN+KYE+ IK+VL GP TK
Sbjct:   22 MSTLSKAEVEDIREVFDLFDFWDGRDGMIDAVKVGDLLRCSGINPTIALTVKHGATTKQGEKQYKFDEFLPCYEAILKEKETGTFADYMEAYKTFDREGQGFISAAEMRHVLTGYGERLDDKEVDQIVKFIDLHEDLDGNIKYEELIKRVLAGPSTK 178          
BLAST of Myosin light chain protein vs. TrEMBL
Match: A0A1S8X1M3 (EF hand OS=Opisthorchis viverrini OX=6198 GN=X801_03489 PE=4 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 1.153e-80
Identity = 116/164 (70.73%), Postives = 138/164 (84.15%), Query Frame = 1
Query:  268 KNRSNSAMTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 759
            + RS+   ++LSK+E+EDIREVFDLFDFWDGRDGM+DA+KVGDLLRC+GMNPT  IC+KHGAT K G KQYKF+EF+P YE I++E E GTFADYMEAFKTFDREGQGFIS AEMRHVLT YGE L + + D I+K  D HED+DGN+KYE+ IKKV++GP  K
Sbjct:  128 RGRSDKLCSSLSKAEVEDIREVFDLFDFWDGRDGMIDAVKVGDLLRCSGMNPTIAICVKHGATRKQGEKQYKFDEFLPCYEAILKENEGGTFADYMEAFKTFDREGQGFISAAEMRHVLTGYGEYLEDSDVDKIVKLIDLHEDLDGNIKYEELIKKVMQGPSAK 291          

HSP 2 Score: 136.346 bits (342), Expect = 3.693e-34
Identity = 64/89 (71.91%), Postives = 75/89 (84.27%), Query Frame = 1
Query:  466 GLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIK 732
            G KQYKFEEF+P YE I++EK +GTFADYMEAFKTFDREGQG +S AEMRHVLT YGEKL E++ D ILKF D  ED++GNVKYED ++
Sbjct:    4 GEKQYKFEEFLPCYEAILKEKAAGTFADYMEAFKTFDREGQGIVSAAEMRHVLTGYGEKLKEDQVDQILKFIDLREDLEGNVKYEDGVQ 92          
BLAST of Myosin light chain protein vs. TrEMBL
Match: A0A1I8JL32 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002188g1 PE=4 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 3.750e-80
Identity = 118/156 (75.64%), Postives = 133/156 (85.26%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKT 756
            MT LSK EIEDIREVFDLFDFWDGRDG VDA KV DLLRC GMNPT   C+KHG TEKMG K YKFEEF+PIY+++++ KE GTFAD+MEAFKTFDREGQG+IS AE+RHVLT YGE+L + + D I+KFTDTHED+DGNVKYEDFIKKV  GPK 
Sbjct:    1 MTGLSKGEIEDIREVFDLFDFWDGRDGEVDAAKVADLLRCVGMNPTIATCLKHGGTEKMGEKGYKFEEFLPIYQEVMKAKEEGTFADFMEAFKTFDREGQGYISAAELRHVLTSYGERLDDSDVDVIMKFTDTHEDLDGNVKYEDFIKKVQAGPKV 156          
BLAST of Myosin light chain protein vs. Ensembl Cavefish
Match: myl6 (myosin light polypeptide 6 [Source:NCBI gene;Acc:103021650])

HSP 1 Score: 114.775 bits (286), Expect = 5.513e-31
Identity = 58/156 (37.18%), Postives = 95/156 (60.90%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M +K   FE+F+P+ + I + K+ G+F D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F+++++ G
Sbjct:    1 MSDFSEDQISEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNIKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRQIMAG 151          
BLAST of Myosin light chain protein vs. Ensembl Cavefish
Match: myl6 (myosin light polypeptide 6 [Source:NCBI gene;Acc:103021650])

HSP 1 Score: 113.235 bits (282), Expect = 2.172e-30
Identity = 58/156 (37.18%), Postives = 94/156 (60.26%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M +K   FE+F+P+ + I + K+ G+F D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:    1 MSDFSEDQISEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNIKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEELVRMVMSG 151          
BLAST of Myosin light chain protein vs. Ensembl Cavefish
Match: zgc:153867 (zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170])

HSP 1 Score: 107.842 bits (268), Expect = 2.558e-28
Identity = 56/152 (36.84%), Postives = 91/152 (59.87%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+ + I + K+ G+F D++E  + FD+EG G +  AE+RHVLT  GEK++E+E + +L     HED +G + YE F++ ++ G
Sbjct:    4 SEDQILEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNHKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEDEVETLLA---GHEDANGCINYEAFVRHIMSG 150          
BLAST of Myosin light chain protein vs. Ensembl Cavefish
Match: cmlc1 (cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-GENE-030522-1])

HSP 1 Score: 104.76 bits (260), Expect = 1.132e-26
Identity = 55/158 (34.81%), Postives = 88/158 (55.70%), Query Frame = 1
Query:  283 SAMTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            S +   +  +IE+ R+ F LFD     +  +   + GD++R  G NPTN    + +     E+M  K   FE F+P+ + I + K+ GTF D++E  + FD+EG G +  AE+RHVL   GE+++E+E D ++      ED +G + Y  FIK +L G
Sbjct:   33 SIVLEFTPDQIEEFRDAFTLFDETPTGEMKIRYAQCGDVMRALGHNPTNADVHVVLGKPKLEEMNSKMLDFETFLPMLQHISRAKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERMTEDEVDRLMV---GQEDANGCINYVSFIKHILSG 187          
BLAST of Myosin light chain protein vs. Ensembl Cavefish
Match: myl4 (myosin light chain 4-like [Source:NCBI gene;Acc:103026687])

HSP 1 Score: 104.375 bits (259), Expect = 1.424e-26
Identity = 54/153 (35.29%), Postives = 87/153 (56.86%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
             S  +IE+ +E F LFD     +  +   + GD++R  G NPTN   ++       E++  K   FE F+P+++ I + KE GTF D++E  + FD+EG G +  AE+RHVL   GEK++E+E + ++      ED  G + YE F+K ++ G
Sbjct:   38 FSLDQIEEFKEAFMLFDKTPNGEMKISYAQCGDVMRALGQNPTNAEVMRVLGKPKPEELNTKLMDFETFLPMFQHISKSKERGTFEDFVEGLRVFDKEGNGTVMGAELRHVLQTLGEKMTEKEVEQVMA---GQEDSSGCINYEAFVKHIMSG 187          
BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Match: cmlc1 (cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-GENE-030522-1])

HSP 1 Score: 99.3673 bits (246), Expect = 8.472e-26
Identity = 56/154 (36.36%), Postives = 90/154 (58.44%), Query Frame = 1
Query:  301 SKSEIEDIREVFDLFDFWDGRDG--MVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            ++ +IE+ RE F LFD    R G   +   + GD++R  G NPTN   +K   +   E+M +K   F+ F+P+ + I + K+ G++ D++E  + FD+EG G +  AE+RHVL   GE+L+E+E + ++      ED  G + YE FIK +L G
Sbjct:    5 TQDQIEEYREAFSLFD----RTGEMKITYSQCGDIMRALGQNPTNCDVMKVLGNPKPEEMLVKTMDFDTFLPMMQAICKIKDQGSYEDFVEGLRVFDKEGNGRVMGAELRHVLATLGERLTEDEVEQLMT---GQEDSSGCINYEAFIKHILAG 151          
BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Match: cmlc1 (cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-GENE-030522-1])

HSP 1 Score: 96.6709 bits (239), Expect = 2.411e-24
Identity = 52/146 (35.62%), Postives = 82/146 (56.16%), Query Frame = 1
Query:  319 DIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            + +E F LFD     D  +   + GD++R  G NPTN   +K       E+M +K   FE F+P  + +   K+ GT  D++E  + FD+EG G +  AE+RHVL   GEK++E+E + ++      ED +G++ YE FIK ++ G
Sbjct:   44 EFKEAFSLFDRTG--DVKITYSQCGDVMRALGQNPTNAEVLKVLNKPKPEEMLIKDMDFETFLPTLQHVANSKDRGTLEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEDEVEQLMS---GQEDSNGDINYEAFIKHIMAG 184          
BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Match: myl1 (myosin, light chain 1, alkali; skeletal, fast [Source:ZFIN;Acc:ZDB-GENE-030131-8109])

HSP 1 Score: 94.7449 bits (234), Expect = 4.713e-24
Identity = 53/147 (36.05%), Postives = 85/147 (57.82%), Query Frame = 1
Query:  313 IEDIREVFDLFDFWDGRDG--MVDALKVGDLLRCNGMNPTNRICIKHGATEK---MGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKV 738
            + D +E F LFD    R G   +   + G+++R  G NPTN   +K     K   M +K  +FE+F+P+ + + + K+ GTF D++E  + FD+EG G +  AE+RHVL   GE++SEEE + +L      ED +G + YE  +++V
Sbjct:    9 LPDFKEAFSLFD----RTGEMKISYSQCGEVMRALGQNPTNAEVLKLLGNPKPAEMNVKMLEFEQFLPMLQTVSKSKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLCTLGERMSEEEVELLLV---GQEDTNGLINYEAEVRQV 148          
BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008102.1 (pep scaffold:Pmarinus_7.0:GL477063:81055:85254:1 gene:ENSPMAG00000007323.1 transcript:ENSPMAT00000008102.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.8037 bits (203), Expect = 2.863e-20
Identity = 40/96 (41.67%), Postives = 61/96 (63.54%), Query Frame = 1
Query:  460 KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            +M  K   FE+F+P+   I + K+ GTF D++E  + FD+EG G +  AE+RHVLT  GEK+SE+E + +L      ED +G + YE FI+ ++ G
Sbjct:    1 EMSTKTLDFEQFLPMLSTIAKSKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMSEDEVELLLV---GQEDNNGCINYEAFIRHIMSG 93          
BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 82.8037 bits (203), Expect = 1.199e-19
Identity = 52/146 (35.62%), Postives = 86/146 (58.90%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN-RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIK 732
            L++ +I + +E F LFD  DG DG +   ++G ++R  G NPT   +       +  G     F EF+ +  + +  K++ +  +  EAF+ FD++G G+IS AE+RHV+T  GEKL++EE D +++  D   D DG V YE+F++
Sbjct:    5 LTEEQIAEFKEAFSLFD-KDG-DGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKM--KDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADI--DGDGQVNYEEFVQ 144          
BLAST of Myosin light chain protein vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 71.2478 bits (173), Expect = 1.155e-15
Identity = 43/134 (32.09%), Postives = 75/134 (55.97%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPT--------NRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILK 675
            L++ +I + +E F LFD     +G + + ++  ++R  G++P+        N I +        G  Q +F EF+ +  +  Q K + +  + +EAFK FD+ G G IS AE++HVLT  GEKL++ E D++L+
Sbjct:    5 LTEEQIAEFKEAFALFD--KDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVD-------GNHQIEFSEFLALMSR--QLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLR 127          
BLAST of Myosin light chain protein vs. Ensembl Yeast
Match: MLC1 (Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition [Source:SGD;Acc:S000003074])

HSP 1 Score: 68.5514 bits (166), Expect = 9.987e-15
Identity = 43/141 (30.50%), Postives = 73/141 (51.77%), Query Frame = 1
Query:  337 DLFDFWDGR-DGMVDALKVGDLLRCNGMNPTNR-----ICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            D+F  +D +  G +    +GD LR  G NPTN+     I       +   L   +    + + EK +         D+++AF+ FD+E  G +S+ ++R++LT  GEKL++ E D +LK  +   D +G + Y+ FI+ VL
Sbjct:    9 DIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTNQLVQDIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVSVGDLRYMLTGLGEKLTDAEVDELLKGVEV--DSNGEIDYKKFIEDVL 147          
BLAST of Myosin light chain protein vs. Ensembl Nematostella
Match: EDO49541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGK2])

HSP 1 Score: 101.293 bits (251), Expect = 3.026e-26
Identity = 57/152 (37.50%), Postives = 87/152 (57.24%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK-HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            M  L++ +I + RE F LFD     DG ++  ++G LLR  G+NPT     K     +  G+ +  FEEF+PIY    Q+K++G+  +++E  + FDR+G G +  AE+R VL   GEKLS+EE   +    D   D  G++ YE+ +K VL
Sbjct:    1 MAGLTEEQIGEYREAFSLFDRVG--DGKIECDQIGCLLRSLGLNPTGAEVKKIEKDVDPKGIARVSFEEFLPIYTSAYQKKQTGSADEFVEGLRVFDRDGTGTVLAAELRSVLMSLGEKLSDEEVTTLFAPVD---DSSGHINYEELVKVVL 147          
BLAST of Myosin light chain protein vs. Ensembl Nematostella
Match: EDO44509 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVT6])

HSP 1 Score: 97.8265 bits (242), Expect = 6.378e-25
Identity = 58/155 (37.42%), Postives = 95/155 (61.29%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN---RICIKHGATEKMGLKQYKFEEFVPIYE----KIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            L++ +I ++ +VF ++D     DG V++ ++G+++R   +NPTN   R  IK    E  G ++  F+EF P+Y+    K+ + K      D++++ K FD +G GFIS  E+RHVLT  GEKLS+EE D++ +  + ++   G V YE+FIK VL
Sbjct:    5 LTERQIAELTDVFSIYDTVG--DGKVESAQLGEVMRAFDLNPTNVEVRKIIKEVDPE--GTRRVSFDEFFPVYQSFKGKVARNKAKSD--DFVDSLKVFDSDGSGFISAGELRHVLTSIGEKLSDEEADSLFQAVEYNQ---GQVNYEEFIKYVL 150          
BLAST of Myosin light chain protein vs. Ensembl Nematostella
Match: EDO44682 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV39])

HSP 1 Score: 92.4337 bits (228), Expect = 5.347e-23
Identity = 59/150 (39.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query:  313 IEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRIC--IKHGATEKMGLKQYKFEEFVPIYEKIIQE--KESGTFADY-MEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            +ED++E F L+D     D  ++  +VG++LR   +NPT      I +       +K+  FEEF P+Y+ + +   KE     DY ME FK FDR G G I  AE+RH+L   G+KLS+EE DN++      ED  G V YEDF++ V+ G
Sbjct:    7 LEDLKECFLLYDKRG--DERIECSQVGEVLRALDVNPTEHEVQKIVNNIDPAGEMKRVSFEEFYPMYQNLRERHRKERSVDVDYFMECFKVFDRNGNGLIGAAELRHLLASLGDKLSDEEVDNLMV---GFEDNQGQVFYEDFVRAVMNG 151          
BLAST of Myosin light chain protein vs. Ensembl Nematostella
Match: EDO37259 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG62])

HSP 1 Score: 91.6633 bits (226), Expect = 1.375e-22
Identity = 52/151 (34.44%), Postives = 89/151 (58.94%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK-HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            L+  +I  +RE F+L+D     D  +D+ ++G++LR  G NPTN    K     +  G  +  FEEF+P+Y+  + +K   +  D++++ + FD +G G I+  E+RH+LT  GEKL++ E D++++     ED  G +  E+FI  VL+G
Sbjct:    5 LTDKDIRQLRESFELYDKQG--DEKIDSSQLGEVLRGLGQNPTNAEVKKIVSEMDPEGGNRISFEEFLPVYQSYMGKKPKFSTEDFVDSLRVFDNDGSGMINEGELRHILTTLGEKLTDSEVDSLIQ---GLEDKQGQIDCEEFILSVLQG 150          
BLAST of Myosin light chain protein vs. Ensembl Nematostella
Match: EDO46518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPI8])

HSP 1 Score: 85.1149 bits (209), Expect = 3.472e-20
Identity = 54/146 (36.99%), Postives = 86/146 (58.90%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN-RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIK 732
            L++ +I + +E F LFD  DG DG +   ++G ++R  G NPT   +       +  G     F EF+ +  + +  K++ +  +  EAF+ FD++G GFIS AE+RHV+T  GEKL++EE D +++  D   D DG V YE+F+K
Sbjct:    5 LTEEQIAEFKEAFSLFD-KDG-DGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKM--KDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADI--DGDGQVNYEEFVK 144          
BLAST of Myosin light chain protein vs. Ensembl Medaka
Match: ENSORLT00000040794.1 (myosin light polypeptide 6 [Source:NCBI gene;Acc:101167617])

HSP 1 Score: 111.694 bits (278), Expect = 9.943e-30
Identity = 58/156 (37.18%), Postives = 93/156 (59.62%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  S+ +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+++ I + K+ G+  D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F++ +L G
Sbjct:    1 MSDFSEDQIIEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNTKMLDFEQFLPMFQAIAKNKDQGSMEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRHILSG 151          
BLAST of Myosin light chain protein vs. Ensembl Medaka
Match: ENSORLT00000009354.2 (myosin light polypeptide 6 [Source:NCBI gene;Acc:101167617])

HSP 1 Score: 110.153 bits (274), Expect = 1.688e-28
Identity = 56/149 (37.58%), Postives = 89/149 (59.73%), Query Frame = 1
Query:  310 EIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+++ I + K+ G+  D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE F++ +L G
Sbjct:   66 QIHEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNTKMLDFEQFLPMFQAIAKNKDQGSMEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEAFVRHILSG 209          
BLAST of Myosin light chain protein vs. Ensembl Medaka
Match: ENSORLT00000030160.1 (myosin light polypeptide 6 [Source:NCBI gene;Acc:101167617])

HSP 1 Score: 108.612 bits (270), Expect = 7.266e-28
Identity = 55/149 (36.91%), Postives = 89/149 (59.73%), Query Frame = 1
Query:  310 EIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            +I + +E F LFD     DG +   + GD++R  G NP N   +K   +   E+M  K   FE+F+P+++ I + K+ G+  D++E  + FD+EG G +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:   66 QIHEFKEAFLLFDRTG--DGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNTKMLDFEQFLPMFQAIAKNKDQGSMEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCINYEELVRMVMSG 209          
BLAST of Myosin light chain protein vs. Ensembl Medaka
Match: ENSORLT00000029441.1 (myosin light polypeptide 6 [Source:NCBI gene;Acc:101160287])

HSP 1 Score: 107.457 bits (267), Expect = 1.416e-27
Identity = 57/156 (36.54%), Postives = 91/156 (58.33%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            M+  +  +I + +EVF LFD     +G +   + GD++R  G NP N   +K   +  +E+M  K   FE+F+P+   I + KE G+F D +E  + FD+EG   +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:   44 MSDFTDDQIMEFKEVFSLFDRTG--EGKITYSQCGDVMRALGQNPVNAEVLKVLGNPKSEEMNYKTLDFEQFLPMLHAITKNKEQGSFEDIVEGLRVFDKEGNSTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCIHYEELVRMVMSG 194          
BLAST of Myosin light chain protein vs. Ensembl Medaka
Match: ENSORLT00000032707.1 (myosin light polypeptide 6 [Source:NCBI gene;Acc:101160287])

HSP 1 Score: 104.76 bits (260), Expect = 3.624e-27
Identity = 55/146 (37.67%), Postives = 86/146 (58.90%), Query Frame = 1
Query:  319 DIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 747
            + +EVF LFD     +G +   + GD++R  G NP N   +K   +  +E+M  K   FE+F+P+   I + KE G+F D +E  + FD+EG   +  AE+RHVLT  GEK++EEE + +L     HED +G + YE+ ++ V+ G
Sbjct:    3 EFKEVFSLFDRTG--EGKITYSQCGDVMRALGQNPVNAEVLKVLGNPKSEEMNYKTLDFEQFLPMLHAITKNKEQGSFEDIVEGLRVFDKEGNSTVMGAELRHVLTTLGEKMTEEEVETLLA---GHEDANGCIHYEELVRMVMSG 143          
BLAST of Myosin light chain protein vs. Planmine SMEST
Match: SMESG000075512.1 (SMESG000075512.1)

HSP 1 Score: 322.783 bits (826), Expect = 2.683e-112
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 759
            MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK
Sbjct:    1 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGPKTK 157          
BLAST of Myosin light chain protein vs. Planmine SMEST
Match: SMESG000042179.1 (SMESG000042179.1)

HSP 1 Score: 98.5969 bits (244), Expect = 6.061e-25
Identity = 54/151 (35.76%), Postives = 88/151 (58.28%), Query Frame = 1
Query:  289 MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 741
            M  L++ E+ D  E F LFD     D  ++A ++G+++R  G+NPT     K G   +   ++  FE F+PIY+ ++++K      +++E FK FD++G G IS AE+RH+LT  GE+L E E D +L      E+  G + YEDF+  ++
Sbjct:    1 MARLTEMELNDALETFALFDTKG--DSKIEAKQIGEVVRALGLNPTEAEIKKCGYNNQD--ERISFEIFIPIYQSLMKQKVVSDPQEFVEGFKVFDKDGNGMISAAELRHLLTAIGERLRESEVDALLA---GMENSQGQISYEDFVHHIM 144          
BLAST of Myosin light chain protein vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.229e-19
Identity = 53/145 (36.55%), Postives = 85/145 (58.62%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN-RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFI 729
            L++ +I + +E F LFD  DG DG +   ++G ++R  G NPT   +       +  G     F EF+ +  + +++ +S    +  EAF+ FD++G GFIS AE+RHV+T  GEKL++EE D +++  D   D DG V YE+F+
Sbjct:    5 LTEEQIAEFKEAFSLFD-KDG-DGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSE--EEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADI--DGDGQVNYEEFV 143          
BLAST of Myosin light chain protein vs. Planmine SMEST
Match: SMESG000010769.1 (SMESG000010769.1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.229e-19
Identity = 53/145 (36.55%), Postives = 85/145 (58.62%), Query Frame = 1
Query:  298 LSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTN-RICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFI 729
            L++ +I + +E F LFD  DG DG +   ++G ++R  G NPT   +       +  G     F EF+ +  + +++ +S    +  EAF+ FD++G GFIS AE+RHV+T  GEKL++EE D +++  D   D DG V YE+F+
Sbjct:    5 LTEEQIAEFKEAFSLFD-KDG-DGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSE--EEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADI--DGDGQVNYEEFV 143          
BLAST of Myosin light chain protein vs. Planmine SMEST
Match: SMESG000042402.1 (SMESG000042402.1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.031e-14
Identity = 50/151 (33.11%), Postives = 87/151 (57.62%), Query Frame = 1
Query:  295 ALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRI----CIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFAD--YMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFI 729
             LS+ +  +IR+ F LFD  +  +G + +  +  +L+C G++P N I    C+K    +  G    +F++F  +  ++++E     F+D    +AF+  D+EG GFI+  E+RHVLT   +KL++EE D ++K  D  +D DG + YE F+
Sbjct:    9 GLSQDQKYEIRQAFLLFDKLN--EGKISSSDLSMVLKCLGVDP-NEIEMLDCLKE--LDPTGSGFIEFDKFNSVIIRMMKE----PFSDEELRDAFRVLDKEGNGFITATEIRHVLTNLCDKLTDEEVDEMIKEAD--DDGDGMINYEIFV 148          
The following BLAST results are available for this feature:
BLAST of Myosin light chain protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MYL6B2.574e-2837.33myosin light chain 6B [Source:HGNC Symbol;Acc:HGNC... [more]
MYL6B2.574e-2837.33myosin light chain 6B [Source:HGNC Symbol;Acc:HGNC... [more]
MYL62.231e-2735.26myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:... [more]
MYL62.231e-2735.26myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:... [more]
MYL65.609e-2735.26myosin light chain 6 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Myosin light chain protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mlc-35.366e-3646.21Myosin, essential light chain [Source:UniProtKB/S... [more]
mlc-35.366e-3646.21Myosin, essential light chain [Source:UniProtKB/S... [more]
mlc-52.465e-2537.41Myosin-2 essential light chain [Source:UniProtKB/... [more]
mlc-52.465e-2537.41Myosin-2 essential light chain [Source:UniProtKB/... [more]
cmd-13.080e-1936.55Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
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BLAST of Myosin light chain protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mlc-c2.559e-2635.95gene:FBgn0004687 transcript:FBtr0070783[more]
Mlc11.314e-2534.19gene:FBgn0002772 transcript:FBtr0344131[more]
Mlc11.314e-2534.19gene:FBgn0002772 transcript:FBtr0085196[more]
Mlc-c3.420e-2536.73gene:FBgn0004687 transcript:FBtr0308579[more]
Mlc17.791e-2534.19gene:FBgn0002772 transcript:FBtr0085195[more]
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BLAST of Myosin light chain protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:1538672.174e-2937.82zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170][more]
zgc:1538674.351e-2937.82zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170][more]
myl64.444e-2936.54myosin, light chain 6, alkali, smooth muscle and n... [more]
myl66.156e-2936.54myosin, light chain 6, alkali, smooth muscle and n... [more]
CR381567.11.410e-2838.16pep chromosome:GRCz11:CHR_ALT_CTG23_1_29:28397866:... [more]
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BLAST of Myosin light chain protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl11.448e-2735.26myosin light chain 1 [Source:NCBI gene;Acc:394551][more]
fa2h2.667e-2738.00fatty acid 2-hydroxylase [Source:Xenbase;Acc:XB-GE... [more]
myl13.323e-2735.53myosin light chain 1 [Source:NCBI gene;Acc:394551][more]
myl13.323e-2735.53myosin light chain 1 [Source:NCBI gene;Acc:394551][more]
abat1.992e-2636.714-aminobutyrate aminotransferase [Source:Xenbase;A... [more]
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BLAST of Myosin light chain protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Myl6b1.557e-2938.67myosin, light polypeptide 6B [Source:MGI Symbol;Ac... [more]
Myl61.532e-2735.26myosin, light polypeptide 6, alkali, smooth muscle... [more]
Myl63.851e-2735.26myosin, light polypeptide 6, alkali, smooth muscle... [more]
Myl44.812e-2737.50myosin, light polypeptide 4 [Source:MGI Symbol;Acc... [more]
Myl44.812e-2737.50myosin, light polypeptide 4 [Source:MGI Symbol;Acc... [more]
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BLAST of Myosin light chain protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P07291|MLE_ARGIR1.228e-6463.64Myosin essential light chain, striated adductor mu... [more]
sp|P05945|MLE_TODPA4.129e-6363.64Myosin catalytic light chain LC-1, mantle muscle O... [more]
sp|P07290|MLE_MIZYE7.579e-6363.58Myosin, essential light chain, adductor muscle OS=... [more]
sp|P53014|MLE_CAEEL6.826e-3546.21Myosin, essential light chain OS=Caenorhabditis el... [more]
sp|P02607|MYL6_CHICK7.373e-2937.82Myosin light polypeptide 6 OS=Gallus gallus OX=903... [more]
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BLAST of Myosin light chain protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
E5Q3363.105e-10698.09Myosin light chain protein OS=Dugesia japonica OX=... [more]
A0A074ZUM94.265e-8170.73Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A5J4NZX38.256e-8173.25Uncharacterized protein (Fragment) OS=Paragonimus ... [more]
A0A1S8X1M31.153e-8070.73EF hand OS=Opisthorchis viverrini OX=6198 GN=X801_... [more]
A0A1I8JL323.750e-8075.64Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Myosin light chain protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl65.513e-3137.18myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
myl62.172e-3037.18myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
zgc:1538672.558e-2836.84zgc:153867 [Source:ZFIN;Acc:ZDB-GENE-030131-9170][more]
cmlc11.132e-2634.81cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-... [more]
myl41.424e-2635.29myosin light chain 4-like [Source:NCBI gene;Acc:10... [more]
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BLAST of Myosin light chain protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cmlc18.472e-2636.36cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-... [more]
cmlc12.411e-2435.62cardiac myosin light chain-1 [Source:ZFIN;Acc:ZDB-... [more]
myl14.713e-2436.05myosin, light chain 1, alkali; skeletal, fast [Sou... [more]
ENSPMAT00000008102.12.863e-2041.67pep scaffold:Pmarinus_7.0:GL477063:81055:85254:1 g... [more]
CALML31.199e-1935.62calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
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BLAST of Myosin light chain protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
CMD11.155e-1532.09Calmodulin; Ca2+ binding protein that regulates Ca... [more]
MLC19.987e-1530.50Essential light chain for Myo1p; light chain for M... [more]
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BLAST of Myosin light chain protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO495413.026e-2637.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445096.378e-2537.42Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO446825.347e-2339.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO372591.375e-2234.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO465183.472e-2036.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Myosin light chain protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000040794.19.943e-3037.18myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
ENSORLT00000009354.21.688e-2837.58myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
ENSORLT00000030160.17.266e-2836.91myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
ENSORLT00000029441.11.416e-2736.54myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
ENSORLT00000032707.13.624e-2737.67myosin light polypeptide 6 [Source:NCBI gene;Acc:1... [more]
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BLAST of Myosin light chain protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000075512.12.683e-112100.00SMESG000075512.1[more]
SMESG000042179.16.061e-2535.76SMESG000042179.1[more]
SMESG000034095.13.229e-1936.55SMESG000034095.1[more]
SMESG000010769.13.229e-1936.55SMESG000010769.1[more]
SMESG000042402.18.031e-1433.11SMESG000042402.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026887 ID=SMED30026887|Name=Myosin light chain protein|organism=Schmidtea mediterranea sexual|type=transcript|length=986bp
CATTCGGGGCTTATTCACCTGGCACATGAAACGGCCGAGTGCAAAATCGT
GCACACCGATTCCACCAGGATTCTGCATGACTATTGATTATTTAGGCGCA
AATCACTCCCAACAGTTCATGCACACTCGAGCAACCAATCAGCATTATCC
GTGTGCACTTTTGGATTATATTAAAGGTCTAACAGAAAATGAATTCTTCA
AACAATTTTCCTGCCCCTTGTCGAAGATAGAAAGCAGAAGTCATAATTTC
GGTTTTATTTTTATCAAAAAAACAGATCAAATTCAGCAATGACAGCCCTC
TCTAAATCCGAAATTGAAGATATTCGAGAAGTTTTCGACCTGTTTGATTT
CTGGGATGGTCGAGATGGTATGGTTGACGCACTCAAAGTTGGAGACTTGT
TACGGTGTAACGGAATGAATCCCACAAACAGGATATGTATAAAACATGGA
GCTACAGAGAAAATGGGATTAAAACAATACAAATTTGAAGAATTCGTTCC
AATCTATGAAAAAATCATCCAAGAAAAAGAAAGTGGAACATTCGCAGATT
ATATGGAAGCTTTTAAAACCTTTGACCGAGAAGGGCAAGGATTCATTTCT
CTTGCGGAGATGAGACACGTGCTGACCATGTATGGAGAAAAGTTGTCAGA
AGAAGAAACAGATAACATTCTCAAGTTTACTGACACACACGAAGACATTG
ATGGAAATGTGAAATATGAGGATTTCATTAAGAAGGTTCTCGAAGGACCA
AAAACCAAGTAACAATTTCTGGATATCATAGTTATCAGAGACCTTTTCCA
GTTCCAACATTGTTCTATTTGATTGCATGAATCGGCTATATTATTTTGCA
TGTGTCTATAACTTGATGCGGTCAGTGTACATCCTGGGGCGTGATCAATT
AATCGTAATTAATAGATTAAATCAATTGTGTAATTTTGCCTACCTTCGTT
AATTTCTCAAATATAACATTGGTCGATCAAAAAAAA
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protein sequence of SMED30026887-orf-1

>SMED30026887-orf-1 ID=SMED30026887-orf-1|Name=SMED30026887-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp
MTALSKSEIEDIREVFDLFDFWDGRDGMVDALKVGDLLRCNGMNPTNRIC
IKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ
GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKV
LEGPKTK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000019muscle progenitor cell
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR029655Mlc1
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..85
e-value: 2.9E-19
score: 71.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 86..154
e-value: 6.8E-19
score: 69.8
NoneNo IPR availablePANTHERPTHR23048MYOSIN LIGHT CHAIN 1, 3coord: 7..155
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 87..148
e-value: 1.1E-7
score: 32.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 83..118
score: 12.142
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 7..44
score: 6.898
IPR002048EF-hand domainCDDcd00051EFhcoord: 87..148
e-value: 2.99881E-9
score: 48.6981
IPR029655Myosin light chain alkaliPANTHERPTHR23048:SF33MYOSIN LIGHT CHAIN ALKALIcoord: 7..155
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 4..151