PlsC domain-containing protein

Overview
NamePlsC domain-containing protein
Smed IDSMED30026859
Length (bp)2426
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PlsC domain-containing protein (SMED30026859) t-SNE clustered cells

Violin plots show distribution of expression levels for PlsC domain-containing protein (SMED30026859) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PlsC domain-containing protein (SMED30026859) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PlsC domain-containing protein (SMED30026859) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026859

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30026859h1SMcG0018332 SmedASXL_001902SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30026859h1SMcG0018332 dd_Smed_v4_8757_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30026859h1SMcG0018332 dd_Smed_v4_8757_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026859h1SMcG0018332 dd_Smed_v4_8757_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026859h1SMcG0018332 dd_Smed_v4_8757_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026859h1SMcG0018332 dd_Smed_v4_8757_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PlsC domain-containing protein vs. Ensembl Zebrafish
Match: gpam (glycerol-3-phosphate acyltransferase, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-101026-1])

HSP 1 Score: 55.4546 bits (132), Expect = 2.809e-7
Identity = 88/431 (20.42%), Postives = 178/431 (41.30%), Query Frame = 2 
Query:  764 PIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKE----SEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHEFI------------PKQFLLKSNSIW--SCNGLRLLSMCTAKFGVVKLTICKPIYYSQ---------------------------------FSSSCDTENSSTLVDTEYQ---IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPE-SLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAFVTETRFSTITNYTDQ-LSVTCDSMNNTISNLVKI--LLSRYFRFLLPCEML 1882
            P+V++  +K  ID++LI  +     I  P    G +  +  + +++ ++G   I+++    S+ K   L   ++      +L  ++ + + L  + +   S   S     +L   V T     + + +IVP+ +S++ I            PK    K+ S+W  +C   R+L      +G V++   +P    +                                 F+   +   +S L D  ++   I  L KH+    +K   IM T++VA LLLY  + ++ + + ++    F++ + ILS +  LGF  +S    +    ++L +C++V++   N   F+    +    + EL FYS+ +    +  +++A + +   R  T  + ++Q  S T  S    I     +   LS      LPC+ L
Sbjct:  223 PLVFLPVHKSHIDYLLITFILFCHNIKAPHIAAGNNLNIPILSTLIRKLGGFFIRRKMDETSDGKKDVLYRSLLHAYTEELLRQQQFLEVYLEGTRSR--SGKPSPARAGMLSIVVDTLCASTIPDVLIVPVGISYDRIIEGNYNSEQLGKPK----KNESLWGIACGVFRMLR---KNYGCVRVDFTQPFSLKEYLDTQRSRHLQASLTLEQILLPIIIATQPDQAVFNGENENHLNSDLSDEPWRRQVIADLAKHVLFSASKSSAIMSTHIVACLLLY--RHRQGVLLSKLVEDFFNMKEEILSRDFDLGFSGNSE-DVVMHALSLLGNCVNVTSTNKN-TEFIISPSNTVPALFELNFYSNGLFHVFIADAIIACSALALLREQTPASASEQNPSSTLLSQERLIRRAAGLSHFLSNEVAVALPCQTL 640
[Close]
BLAST of PlsC domain-containing protein vs. TrEMBL
Match: N6SYL6 (PlsC domain-containing protein (Fragment) OS=Dendroctonus ponderosae OX=77166 GN=109545486 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.574e-8
Identity = 93/478 (19.46%), Postives = 208/478 (43.51%), Query Frame = 2 
Query:  461 QDDSVHRFI--FELSEVECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESE-MKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFT------EIRVLQSAVKTFIDGKLEEPIIVPLILSHE------FIPKQFLLKSNSIWSCNGLR-LLSMCTAKFGVVKLTICKPIYYSQFSSSCDTEN----------------------SSTLVDTEYQ--IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYI-LSPESLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAF-VTETRFSTIT 1768
            +DD V + +    + +++ +D  ++    ++ ++  A  K+   ++ +  +D+L++F S +  + L  F+ S         + H  Q       +  + +   P++++  ++  +D++L+  +     I  P+   G++ ++    S+L  +G   IK+  + M   K   +        +L    ++CL    ++  +     + T      +  VL   V+TF+DG +E+ ++VP+ L++E      FI +Q           + L+ +  +  + +G +++   +P    +   + D  N                       + +V  E++  ++ + KH+     +   +M TN +AFLLL  F+  + I++ ++  A  DL K + LS   +GF     +  I     +L   + V   +LN +  +       + I EL +YS+ +V    ++SV+A A  V E R +T++
Sbjct:  145 RDDRVKKAVERVAVQQLKDSDSTEDEFYQELIKKNQARAKKLVSDMRSTLSDFLLRFTSWILYKLLPCFMNSV--------VVHPGQVDM----LKQASKTNLPLIFLPLHRSHLDYILLSFILLNNDIRSPLVAAGDNLRIPFFGSLLRGLGAFFIKRRIDPMMGRKDHVY------KAVLHTYMNLCLRSGHNIECFLEGGRTRTGKPCMPKYGVLSVIVETFMDGTIEDALLVPVSLNYEKLIDGNFIKEQLGEPKKMETFGDALKGIWHVLNSSYGSMRVDFNQPFSLRELIKTFDQNNKISNPNVSARVLKSNPSTNSLYGTDVVSEEHKSLVENISKHVIYDCAQSTSVMSTNALAFLLLSKFR--QGITLQKLVVAFEDLKKDLELSRRDVGFS-GDAVDVINYAVELLGPAL-VKKEKLNGEDTIKPIAILPNVI-ELSYYSNSLVTHFALESVIALALSVAEIRLNTVS 599
[Close]
BLAST of PlsC domain-containing protein vs. TrEMBL
Match: U4UFK2 (PlsC domain-containing protein OS=Dendroctonus ponderosae OX=77166 GN=D910_10073 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.635e-8
Identity = 93/478 (19.46%), Postives = 207/478 (43.31%), Query Frame = 2 
Query:  461 QDDSVHRFI--FELSEVECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKE-SEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFT------EIRVLQSAVKTFIDGKLEEPIIVPLILSHE------FIPKQFLLKSNSIWSCNGLR-LLSMCTAKFGVVKLTICKPIYYSQFSSSCDTEN----------------------SSTLVDTEYQ--IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYI-LSPESLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAF-VTETRFSTIT 1768
            +DD V + +    + +++ +D  ++    ++ ++  A  K+   ++ +  +D+L++F S +  + L  F+ S         + H  Q       +  + +   P++++  ++  +D++L+  +     I  P+   G++ ++    S+L  +G   IK+    M   K   +        +L    ++CL    ++  +     + T      +  VL   V+TF+DG +E+ ++VP+ L++E      FI +Q           + L+ +  +  + +G +++   +P    +   + D  N                       + +V  E++  ++ + KH+     +   +M TN +AFLLL  F+  + I++ ++  A  DL K + LS   +GF     +  I     +L   + V   +LN +  +       + I EL +YS+ +V    ++SV+A A  V E R +T++
Sbjct:  145 RDDRVKKAVERVAVQQLKDSDSTEDEFYQELIKKNQARAKKLVSDMRSTLSDFLLRFTSWILYKLLPCFMNSV--------VVHPGQVDM----LKQASKTNLPLIFLPLHRSHLDYILLSFILLNNDIRSPLVAAGDNLRIPFFGSLLRGLGAFFIKRRIDPMMGRKDHVY------KAVLHTYMNLCLRSGHNIECFLEGGRTRTGKPCMPKYGVLSVIVETFMDGTIEDALLVPVSLNYEKLIDGNFIKEQLGEPKKMETFGDALKGIWHVLNSSYGSMRVDFNQPFSLRELIKTFDQNNKISNPNVSARVLKSNPSTNSLYGTDVVSEEHKSLVENISKHVIYDCAQSTSVMSTNALAFLLLSKFR--QGITLQKLVVAFEDLKKDLELSRRDVGFS-GDAVDVINYAVELLGPAL-VKKEKLNGEDTIKPIAILPNVI-ELSYYSNSLVTHFALESVIALALSVAEIRLNTVS 599
[Close]
BLAST of PlsC domain-containing protein vs. TrEMBL
Match: A0A3Q1JI28 (Glycerol-3-phosphate acyltransferase 1, mitochondrial OS=Anabas testudineus OX=64144 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.112e-7
Identity = 80/345 (23.19%), Postives = 152/345 (44.06%), Query Frame = 2 
Query:  764 PIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESE-----MKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHEFI------------PKQFLLKSNSIW--SCNGLRLLSMCTAKFGVVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQ--IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPE-SLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVAT 1732
            P+V++  +K  ID++LI L+     I  P    G +  +  + +++ ++G   I+++ E      K+   +S +   K  +I +    I L    S     S   S     +L   V T  +G + + ++VP+ +S++ I            PK    K+ S W  +C   R   M    +G V++   +P     FS   +  N     D   +  I  L KH+    NK   IM T++VA LLLY  + ++ + + ++    F++ + ILS +  LGF  +S    + R  ++L +C++V+ +   +  F          + EL FYS+ +    +  +++ T
Sbjct:  170 PMVFLPVHKSHIDYLLITLILFCHNIKAPHIAAGNNLSIPVLSTLIRKLGGFFIRRKMEETATGKKDILYRSLLHAVKEMLIQQQFLEIYLEGTRSR----SGKPSVPRAGMLSIVVDTMCNGSISDVLVVPVGISYDRIIEGNYNSEQLGKPK----KNESWWGIACGVFR---MMRKNYGCVRVDFNQP-----FSLKEEQMNRELPDDISRRQLINNLAKHVLFTANKSSAIMSTHIVACLLLY--RHRQGVVLSKLVEDFFNMKEEILSRDFDLGFSGNSE-DVVMRALHLLGNCVNVTGSANRNGEFTIAPSQTVPALFELNFYSNGLFHVFISDAIIGT 495
[Close]
BLAST of PlsC domain-containing protein vs. TrEMBL
Match: A0A5N4AIF7 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_08060 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.766e-7
Identity = 111/499 (22.24%), Postives = 214/499 (42.89%), Query Frame = 2 
Query:  527 ENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFT------EIRVLQSAVKTFIDGKLEEPIIVPLILSHE------FIPKQ---------FLLKSNSIWSCNGLRLLSMCTAKFGVVKLTICKPIYYSQ--------------FSSSCDTENS-STLVDTE-------YQIKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPE-SLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAF-VTETRFSTITNYTDQLSVTCDSMNNTISNLVKILLSRYFRFLLPCEMLDN 1888
            E LL++   E+ A  K+ F+++ ++ +D L K  S +  + L  F+ S         + HS Q K     +  + Q   P++++  ++  +D+++I  +     I  P+   G++ ++    S+L  +G   I++  +   P L       KA  IL +  +  L    +V  +     + T      +  +    + + +DG +E+ ++VP+ +++E      FI +Q         F     SIW         +  + +G+ ++ + +PI   +              F  S  T  S S+L  T+       Y ++K+ KH+     +   +M TN VA+LLL  F+  K  ++ E+  A  +L   + + +  +GF  SS    I    +ML   +S+   ++ DD F          + EL +YS+I+V    +++V+ATA    +T    ++   D+L   C  + N        LL   F F  PC+ L+ 
Sbjct:  205 EQLLVQ--NERRA--KKLFKDMQSSMSDLLFKLTSWVLYKLLPCFLSSI--------VVHSGQVKM----IKEAGQTGLPLIFLPLHRSHLDYIMISFILFNNNIQCPLIAAGDNLRMPIFGSLLRGLGAFYIRRRLD---PVLGQKDYVYKA--ILHSYMNAGLRAGHNVEFFLEGGRTRTGKPLMPKYGIFSVILDSLMDGTIEDALLVPVSINYEKLVDGNFIREQLGQPKKMETFRSTMRSIWH--------VLNSNYGMARIDLNQPISLRELMKTFNNGKVQNQVFPKSLRTNPSNSSLYGTDVVSDEYKYLVEKISKHVLYDGARSTSVMSTNAVAYLLLRQFR--KGATLDELVAALDELRVDLDNKQKDVGFSGSSR-DAILYAVDMLG--VSLIKKEVIDDKFFITPDTSLPNVIELNYYSNILVNYYCLEAVIATALNCMDTELGEVSE--DELVEGCLDICN--------LLQYEFIFCKPCQDLEG 659
[Close]
BLAST of PlsC domain-containing protein vs. TrEMBL
Match: A0A1E1XDC4 (Putative mitochondrial glycerol-3-phosphate acyltransferase gpat (Fragment) OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 4.742e-7
Identity = 103/507 (20.32%), Postives = 197/507 (38.86%), Query Frame = 2 
Query:  581 FENLATNFNDYLMK----FLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNPK---LQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHE------FIPKQ---------FLLKSNSIWSCNGLRLLSMCTAKFGVVKLTICKPIYYSQF---SSSCDTE-------------NSSTLVDTEYQ---------------------IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFK-----DQKSISIIEISRACFDLNKYILSPESLGFEFSSVITTIRRGCNMLSHCI----------SVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAFVTETR-FSTITNYTDQLSVTCDS-MNNTISNLVKILLSRYFRFLLPC 1873
            FE +  + +  L++    F+  +  R L+   +    +A+L                  +V+   P+VY+  ++  +D++L+  L    G+  P+   G++  +    ++L  +G   IK+  + KN +   L   ++++    IL+   S+   L    +   +      +  +L   V    +G +++  IVP+ +S+E      F+ +Q         F     SIW     R+L    + +G V++  C+PI   +F   S +  TE               S++  TEY+                     IK L  H+   ++    +M TNL+AFLLL   +      Q + S+  +  A  +    I+ P       +     +R  C +L   +          S    + N             ++ EL +YS+ ++   + +++VATA  T T  +  +    D L V   S + N   +L+ I L   F  +LPC
Sbjct:  210 FERMRASISTVLLRVAVWFMHRIMSRMLTGVYVPKGQIAML----------------KQAVEKNVPMVYLPLHRSHLDYILVTYLLYLNGMRVPLVAAGDNLLIPLFGTLLRGLGGFFIKRRLDFKNGRKDHLYRAVLKEYMKEILKGNHSLEFFLEGGRSR--TGKTRLPKAGLLSVIVNCVNEGLVKDVYIVPISISYEKLLDGNFVSEQLGEPKVMETFTAALTSIW-----RILH---SNYGSVRVDFCQPISLQEFLESSKAVSTEPLCFDCPLSLLDVTRSSISPTEYKECASTSSLASADSLPEDCRLAIKSLASHVTCQSSASLALMSTNLLAFLLLTKHRKGGTLQQLAYSLSWLRMAVSERQHQIMCP-------ADSAEAVRHACALLGKELVLTETVQMEWSSGDTENNVKIVFYRPALHLPSVLELQYYSNAVLSVFVHEAIVATALFTLTGDYIALMRGRDNLVVVSRSDLLNKCQDLINI-LQLEFVLVLPC 682
[Close]
BLAST of PlsC domain-containing protein vs. Ensembl Medaka
Match: gpam (glycerol-3-phosphate acyltransferase, mitochondrial [Source:NCBI gene;Acc:101174228])

HSP 1 Score: 57.3806 bits (137), Expect = 4.985e-8
Identity = 72/349 (20.63%), Postives = 152/349 (43.55%), Query Frame = 2 
Query:  764 PIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESE----MKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHEFI------------PKQFLLKSNSIW--SCNGLRLLSMCTAKFGVVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQIKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPE-SLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAFVTETR 1753
            P++++  +K  ID++LI L+     I  P    G +  +  +  ++ ++G   I++  E     K   L   ++      +++ ++ + + L  + +   S   S     +L   V T   G + + ++VP+ +S++ I            PK    K+ S W  +C   R+L      +G V++   +P    ++    DT+ +                     NK   IM T++VA LLLY  + ++ + + ++    F++ + ILS +  LGF  +S    + R  ++L +CI+V+++   +  F          + EL FYS+ +    +  +V+A + ++  R
Sbjct:  217 PMIFLPVHKSHIDYLLITLILFCHNIKAPHIAAGNNLSIPVLSVLIRKLGGFFIRRRMEETEDGKKDVLYRSLLHSYVEELMKQQQFLEVYLEGTRSR--SGKPSTARAGMLSIVVDTLCTGAIGDVLVVPVGISYDRIIEGNYNSEQLGKPK----KNESWWGIACGVFRMLR---KNYGCVRVDFNQPFSLKEY---LDTQRNPA-------------------NKSSSIMSTHIVACLLLY--RHRQGVVLSKLVEDFFNMKEEILSRDFDLGFSGNSE-DVVMRALHLLENCINVTSSSSGNGEFTIAPGQTVPALFELNFYSNCLFHVFISDAVIACSILSLQR 531
[Close]
BLAST of PlsC domain-containing protein vs. Ensembl Medaka
Match: gpam (glycerol-3-phosphate acyltransferase, mitochondrial [Source:NCBI gene;Acc:101174228])

HSP 1 Score: 56.225 bits (134), Expect = 1.114e-7
Identity = 77/383 (20.10%), Postives = 159/383 (41.51%), Query Frame = 2 
Query:  764 PIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESE----MKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHEFI------------PKQFLLKSNSIW--SCNGLRLLSMCTAKFGVVKLTICKPIYYSQ-------------------------------FSSSCDTENSSTLVDTEYQ---IKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPE-SLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAFVTETR 1753
            P++++  +K  ID++LI L+     I  P    G +  +  +  ++ ++G   I++  E     K   L   ++      +++ ++ + + L  + +   S   S     +L   V T   G + + ++VP+ +S++ I            PK    K+ S W  +C   R+L      +G V++   +P    +                               F    + +    L +  Y+   I  L KH+    NK   IM T++VA LLLY  + ++ + + ++    F++ + ILS +  LGF  +S    + R  ++L +CI+V+++   +  F          + EL FYS+ +    +  +V+A + ++  R
Sbjct:  216 PMIFLPVHKSHIDYLLITLILFCHNIKAPHIAAGNNLSIPVLSVLIRKLGGFFIRRRMEETEDGKKDVLYRSLLHSYVEELMKQQQFLEVYLEGTRSR--SGKPSTARAGMLSIVVDTLCTGAIGDVLVVPVGISYDRIIEGNYNSEQLGKPK----KNESWWGIACGVFRMLR---KNYGCVRVDFNQPFSLKEYLDTQRNRHLPPPLSLENTLMPTIISEQPELFEGQEEEQADRELPEEIYRRQLINNLAKHVLFTANKSSSIMSTHIVACLLLY--RHRQGVVLSKLVEDFFNMKEEILSRDFDLGFSGNSE-DVVMRALHLLENCINVTSSSSGNGEFTIAPGQTVPALFELNFYSNCLFHVFISDAVIACSILSLQR 586
[Close]
BLAST of PlsC domain-containing protein vs. Planmine SMEST
Match: SMESG000028760.1 (SMESG000028760.1)

HSP 1 Score: 1542.32 bits (3992), Expect = 0.000e+0
Identity = 784/788 (99.49%), Postives = 784/788 (99.49%), Query Frame = 2 
Query:   53 MATHVNGLNFRRNHFNDGEVPKINIXXXXXXXXXXXXXXXNDNNNDVLIKSLCDPKYNYEKTQVNQMKNGNDNGLCGCWYIFNQFVKKEIQESIDSDGFNDINIFSRNTMYRFRTFLNSNKDCERFLDPVSWESFSQDDSVHRFIFELSEVECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVKTFIDGKLEEPIIVPLILSHEFIPKQFLLKSNSIWSCNGLRLLSMCTAKFGVVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQIKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPESLGFEFSSVITTIRRGCNMLSHCISVSANQLNXXXXXXXXXXXFSTISELGFYSDIIVQKLLMKSVVATAFVTETRFSTITNYTDQLSVTCDSMNNTISNLVKILLSRYFRFLLPCEMLDNETEKLNSWWNEVMVSSFKESDVLYIDCEFRSELPNWRTDVYDKRVRDQMLMLSRCLAPRLESLNLIVMELMQNKRTGFDSMTICSELYQKFLETAKIASKSKYLRYKNCINPDYFTSGIITLAAHEMIRIFDISRREWVSKPDNVDLLSSRYAILDSEYYNLQRWKTFIDLSLTAA 2416
            MATHVNGLNFRRNHFNDGEVPKINIEQVLELKRQRLLEKENDNNNDVLIKSLCDPKYNYEKTQVNQMKNGNDNGLCGCWYIFNQFVKKEIQESIDSDGFNDINIFSRNTMYRFRTFLNSNKDCERFLDPVSWESFSQDDSVHRFIFELSEVECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVL SAVKTFIDGKLEEPIIVPLILSHEFIPKQFLLKSNSIWSCNGLRLLSMCTAKFGVVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQIKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPESLGFEFSSVI TIRRGCNMLSHCISVS NQLNDDCFLDVDWDDFSTISELGFYSDIIVQKLLMKSVVATAFVTETRFSTITNYTDQLSVTCDSMN TISNLVKILLSRYFRFLLPCEMLDNETEKLNSWWNEVMVSSFKESDVLYIDCEFRSELPNWRTDVYDKRVRDQMLMLSRCLAPRLESLNLIVMELMQNKRTGFDSMTICSELYQKFLETAKIASKSKYLRYKNCINPDYFTSGIITLAAHEMIRIFDISRREWVSKPDNVDLLSSRYAILDSEYYNLQRWKTFIDLSLTAA
Sbjct:    1 MATHVNGLNFRRNHFNDGEVPKINIEQVLELKRQRLLEKENDNNNDVLIKSLCDPKYNYEKTQVNQMKNGNDNGLCGCWYIFNQFVKKEIQESIDSDGFNDINIFSRNTMYRFRTFLNSNKDCERFLDPVSWESFSQDDSVHRFIFELSEVECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSLSSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAIDFVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNPKLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLHSAVKTFIDGKLEEPIIVPLILSHEFIPKQFLLKSNSIWSCNGLRLLSMCTAKFGVVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQIKKLVKHLAIFNNKCQLIMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPESLGFEFSSVIATIRRGCNMLSHCISVSTNQLNDDCFLDVDWDDFSTISELGFYSDIIVQKLLMKSVVATAFVTETRFSTITNYTDQLSVTCDSMNYTISNLVKILLSRYFRFLLPCEMLDNETEKLNSWWNEVMVSSFKESDVLYIDCEFRSELPNWRTDVYDKRVRDQMLMLSRCLAPRLESLNLIVMELMQNKRTGFDSMTICSELYQKFLETAKIASKSKYLRYKNCINPDYFTSGIITLAAHEMIRIFDISRREWVSKPDNVDLLSSRYAILDSEYYNLQRWKTFIDLSLTAA 788
The following BLAST results are available for this feature:
BLAST of PlsC domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gpam2.809e-720.42glycerol-3-phosphate acyltransferase, mitochondria... [more]
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BLAST of PlsC domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
N6SYL63.574e-819.46PlsC domain-containing protein (Fragment) OS=Dendr... [more]
U4UFK23.635e-819.46PlsC domain-containing protein OS=Dendroctonus pon... [more]
A0A3Q1JI282.112e-723.19Glycerol-3-phosphate acyltransferase 1, mitochondr... [more]
A0A5N4AIF73.766e-722.24Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A1E1XDC44.742e-720.32Putative mitochondrial glycerol-3-phosphate acyltr... [more]
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BLAST of PlsC domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PlsC domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gpam4.985e-820.63glycerol-3-phosphate acyltransferase, mitochondria... [more]
gpam1.114e-720.10glycerol-3-phosphate acyltransferase, mitochondria... [more]
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BLAST of PlsC domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000028760.10.000e+099.49SMESG000028760.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026859 ID=SMED30026859|Name=PlsC domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2426bp
GAAAATTTTAAATAGATATTTTTGATAATTTCTTTAGATCAGAACAATTA
AAATGGCTACACATGTCAACGGGCTTAATTTTCGAAGAAATCATTTCAAT
GACGGTGAAGTGCCCAAAATCAACATTGAACAAGTTTTAGAACTGAAAAG
ACAGAGATTATTGGAAAAAGAAAACGATAATAACAATGACGTATTAATTA
AAAGTTTGTGTGATCCCAAATATAATTACGAAAAGACACAAGTAAATCAA
ATGAAAAATGGAAATGATAATGGTCTTTGCGGTTGTTGGTATATATTTAA
TCAATTTGTTAAGAAGGAAATTCAGGAAAGTATTGACTCTGATGGATTTA
ATGATATAAACATATTCAGTCGGAATACTATGTATCGATTTCGAACATTT
CTAAATTCAAATAAGGATTGTGAGAGATTTTTAGACCCAGTTAGTTGGGA
ATCGTTTTCTCAAGACGACAGCGTGCATCGTTTTATTTTCGAACTATCCG
AAGTTGAGTGCACAGATTTAATGGATGAAAATTTATTAATAAAAATATCT
GAAGAAAAATCTGCTTTACTTAAGCAATATTTTGAAAATTTAGCAACTAA
TTTTAATGATTACTTAATGAAGTTTTTGTCAAATTTATGCGAAAGATCTC
TTAGTTCATTCGTTATAAGCACTCATGATTTGGCCTTGTTGTACGGCATT
CACCATTCTAGTCAGTCTAAATCTCCGAACAGTCCCGTTCACAGTTCTGT
GCAAGTGAAACGTCCTATTGTGTATGTTATCAAATATAAATGCGCGATTG
ATTTCGTTCTCATTCCTTTACTATTCAAAGAAGCTGGGATTGATCCACCA
ATTTATTTTGTCGGTGAATCAAAACAAGTCTTAAATATGAAATCAATTTT
AAATCGAATCGGCTACATCATTATTAAAAAAGAATCCGAAATGAAAAATC
CAAAACTCCAATCATTTATTGTTGAAAAAAAAGCTTCTATGATTTTGGAA
GCCAAAAGATCAATTTGCTTAGTACTAAATGATTCTGTAAATACATATTG
CTCTGCAAGTGAATCATTTACAGAAATTAGGGTTTTGCAAAGTGCTGTTA
AAACATTTATTGATGGAAAGCTTGAAGAACCAATTATTGTTCCACTGATT
TTAAGTCATGAATTTATTCCGAAACAATTTTTATTGAAGTCAAACTCGAT
ATGGTCTTGCAATGGTCTGCGATTGTTAAGTATGTGCACTGCTAAATTTG
GCGTAGTTAAACTGACGATCTGTAAACCAATATACTACAGTCAATTTTCA
TCGAGTTGCGATACAGAAAACTCGTCAACATTAGTTGATACAGAATATCA
AATAAAGAAACTAGTGAAACATCTGGCTATTTTTAACAATAAATGCCAAC
TAATAATGCCTACAAATCTTGTTGCATTCTTACTGCTCTATCATTTCAAG
GATCAAAAATCGATATCAATTATCGAAATAAGTCGAGCATGTTTTGATTT
AAACAAATATATTCTTTCTCCCGAATCTCTGGGTTTCGAATTCTCTTCCG
TTATTACTACAATTAGAAGAGGTTGTAATATGTTGTCACACTGTATCAGT
GTTTCTGCCAATCAATTGAATGACGACTGCTTTTTGGATGTTGATTGGGA
TGATTTTTCTACAATTTCCGAATTAGGATTTTATTCTGACATAATCGTTC
AGAAATTGTTAATGAAATCAGTTGTTGCAACAGCTTTTGTAACGGAAACT
CGATTCTCCACCATTACTAATTACACTGATCAATTGAGTGTCACGTGTGA
CTCAATGAATAATACAATCTCAAATTTAGTGAAGATTTTGTTGTCTAGAT
ATTTTCGATTTCTCTTGCCCTGTGAAATGTTAGACAATGAAACAGAAAAG
TTAAACAGTTGGTGGAATGAGGTAATGGTGAGTTCTTTTAAAGAATCAGA
TGTACTTTATATTGATTGTGAATTTCGATCTGAATTACCTAATTGGAGAA
CTGATGTATATGATAAGCGTGTAAGAGATCAGATGTTAATGCTATCACGG
TGCTTAGCTCCGCGATTAGAATCCTTGAATCTCATTGTTATGGAATTAAT
GCAAAACAAACGAACTGGATTTGACTCAATGACAATATGTTCCGAGTTGT
ATCAGAAATTTCTCGAAACTGCTAAAATAGCATCGAAATCGAAATATTTA
CGATATAAGAATTGTATCAATCCCGACTATTTTACAAGTGGAATCATTAC
TCTCGCAGCTCATGAAATGATTAGAATTTTTGATATTTCTCGCAGAGAAT
GGGTTTCGAAACCGGACAATGTAGATTTATTATCTTCTCGGTATGCAATT
TTAGATTCTGAATACTACAATTTACAACGGTGGAAAACTTTCATAGATTT
ATCGTTGACCGCAGCTTAAATTGTTT
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protein sequence of SMED30026859-orf-1

>SMED30026859-orf-1 ID=SMED30026859-orf-1|Name=SMED30026859-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=789bp
MATHVNGLNFRRNHFNDGEVPKINIEQVLELKRQRLLEKENDNNNDVLIK
SLCDPKYNYEKTQVNQMKNGNDNGLCGCWYIFNQFVKKEIQESIDSDGFN
DINIFSRNTMYRFRTFLNSNKDCERFLDPVSWESFSQDDSVHRFIFELSE
VECTDLMDENLLIKISEEKSALLKQYFENLATNFNDYLMKFLSNLCERSL
SSFVISTHDLALLYGIHHSSQSKSPNSPVHSSVQVKRPIVYVIKYKCAID
FVLIPLLFKEAGIDPPIYFVGESKQVLNMKSILNRIGYIIIKKESEMKNP
KLQSFIVEKKASMILEAKRSICLVLNDSVNTYCSASESFTEIRVLQSAVK
TFIDGKLEEPIIVPLILSHEFIPKQFLLKSNSIWSCNGLRLLSMCTAKFG
VVKLTICKPIYYSQFSSSCDTENSSTLVDTEYQIKKLVKHLAIFNNKCQL
IMPTNLVAFLLLYHFKDQKSISIIEISRACFDLNKYILSPESLGFEFSSV
ITTIRRGCNMLSHCISVSANQLNDDCFLDVDWDDFSTISELGFYSDIIVQ
KLLMKSVVATAFVTETRFSTITNYTDQLSVTCDSMNNTISNLVKILLSRY
FRFLLPCEMLDNETEKLNSWWNEVMVSSFKESDVLYIDCEFRSELPNWRT
DVYDKRVRDQMLMLSRCLAPRLESLNLIVMELMQNKRTGFDSMTICSELY
QKFLETAKIASKSKYLRYKNCINPDYFTSGIITLAAHEMIRIFDISRREW
VSKPDNVDLLSSRYAILDSEYYNLQRWKTFIDLSLTAA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR022284GPAT/DHAPAT
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
GO:0044255cellular lipid metabolic process
Vocabulary: molecular function
TermDefinition
GO:0008374O-acyltransferase activity
GO:0016740transferase activity
GO:0016746transferase activity, transferring acyl groups
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022284Glycerol-3-phosphate O-acyltransferase/Dihydroxyacetone phosphate acyltransferasePANTHERPTHR12563GLYCEROL-3-PHOSPHATE ACYLTRANSFERASEcoord: 229..743