Venom allergen 5
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Homology
BLAST of Venom allergen 5 vs. Ensembl Human
Match: GLIPR1L1 (GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392]) HSP 1 Score: 69.707 bits (169), Expect = 3.912e-15 Identity = 38/108 (35.19%), Postives = 62/108 (57.41%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKY---GGFESVGQNVATYSGKNIFRGEEL--WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A++M+ M+W+ A A+ + ++C F ++ D+ Y FE VG+N+ K+ + WYNE + F + +CS + CGHYTQ+VWA+S +GC +A C Sbjct: 50 PPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAAFEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSCS-RVCGHYTQLVWANSFYVGCAVAMC 156
BLAST of Venom allergen 5 vs. Ensembl Human
Match: GLIPR1L1 (GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392]) HSP 1 Score: 69.707 bits (169), Expect = 4.050e-15 Identity = 38/108 (35.19%), Postives = 62/108 (57.41%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKY---GGFESVGQNVATYSGKNIFRGEEL--WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A++M+ M+W+ A A+ + ++C F ++ D+ Y FE VG+N+ K+ + WYNE + F + +CS + CGHYTQ+VWA+S +GC +A C Sbjct: 50 PPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAAFEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSCS-RVCGHYTQLVWANSFYVGCAVAMC 156
BLAST of Venom allergen 5 vs. Ensembl Human
Match: GLIPR1 (GLI pathogenesis related 1 [Source:HGNC Symbol;Acc:HGNC:17001]) HSP 1 Score: 69.3218 bits (168), Expect = 8.398e-15 Identity = 38/109 (34.86%), Postives = 59/109 (54.13%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTK---DDRKYGGFESVGQNVATYSGKNIFRGEEL--WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 +P AS+M M W+ A A+ + C F+++T+ + + F S+G+N+ T S + WY+E+ + F C K CGHYTQVVWADS ++GC + C Sbjct: 49 KPTASDMLYMTWDPALAQIAKAWASNCQFSHNTRLKPPHKLHPNFTSLGENIWTGSVPIFSVSSAITNWYDEIQDYDFKTRICK-KVCGHYTQVVWADSYKVGCAVQFC 156
BLAST of Venom allergen 5 vs. Ensembl Human
Match: R3HDML (R3H domain containing like [Source:HGNC Symbol;Acc:HGNC:16249]) HSP 1 Score: 65.0846 bits (157), Expect = 3.190e-13 Identity = 37/114 (32.46%), Postives = 60/114 (52.63%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSG--KNIFRGEELWYNEVFGFTFS-----NNTCSLK----SCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + +CI+A+ +Y VGQN++ +SG +++ + W E + + F N C + +C HYTQ+VWA S+ LGC + C Sbjct: 79 PPAANMEYMVWDKRLARAAEAWATQCIWAHGPSQLMRY-----VGQNLSIHSGQYRSVVDLMKSWSEEKWHYLFPAPRDCNPHCPWRCDGPTCSHYTQMVWASSNRLGCAIHTC 187
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245]) HSP 1 Score: 65.4698 bits (158), Expect = 4.890e-13 Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNV--ATYSGKNIFRGEELWYNEVFGFTFSNNTCSL-KSCGHYTQVVWADSSELGCGLAKC 346 P AS+M M W+ + A A+ Y +C++ ++ + R+ G+N+ T G ++ E W++E + S TCS + CGHYTQVVWA + +GCG C Sbjct: 49 PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFC 147
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1) HSP 1 Score: 62.003 bits (149), Expect = 1.752e-12 Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 Query: 17 YGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + R+ AS+M + W+ + A AQ + D C F R G +VG+N+ N +W+NEV N CGHY QVVWA ++ +GCG ++C Sbjct: 88 HNRYRRMVPASDMNMLYWSDELAASAQRHADTCDF-------RHSRGRINVGENIWAAPYSNYSDAISIWFNEVHNPRCGCNHAYKHCCGHYVQVVWAKTNLVGCGFSRC 190
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1) HSP 1 Score: 62.003 bits (149), Expect = 1.886e-12 Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 Query: 17 YGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + R+ AS+M + W+ + A AQ + D C F R G +VG+N+ N +W+NEV N CGHY QVVWA ++ +GCG ++C Sbjct: 88 HNRYRRMVPASDMNMLYWSDELAASAQRHADTCDF-------RHSRGRINVGENIWAAPYSNYSDAISIWFNEVHNPRCGCNHAYKHCCGHYVQVVWAKTNLVGCGFSRC 190
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1) HSP 1 Score: 62.003 bits (149), Expect = 1.980e-12 Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 Query: 17 YGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + R+ AS+M + W+ + A AQ + D C F R G +VG+N+ N +W+NEV N CGHY QVVWA ++ +GCG ++C Sbjct: 88 HNRYRRMVPASDMNMLYWSDELAASAQRHADTCDF-------RHSRGRINVGENIWAAPYSNYSDAISIWFNEVHNPRCGCNHAYKHCCGHYVQVVWAKTNLVGCGFSRC 190
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5478005:1 gene:WBGene00003055.1 transcript:F48E8.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1) HSP 1 Score: 62.003 bits (149), Expect = 1.980e-12 Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 Query: 17 YGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + R+ AS+M + W+ + A AQ + D C F R G +VG+N+ N +W+NEV N CGHY QVVWA ++ +GCG ++C Sbjct: 88 HNRYRRMVPASDMNMLYWSDELAASAQRHADTCDF-------RHSRGRINVGENIWAAPYSNYSDAISIWFNEVHNPRCGCNHAYKHCCGHYVQVVWAKTNLVGCGFSRC 190
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-5 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q18543]) HSP 1 Score: 53.9138 bits (128), Expect = 8.004e-10 Identity = 39/131 (29.77%), Postives = 61/131 (46.56%), Query Frame = 2 Query: 2 RRAVAYGRF----PGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNV------ATYSGKNIF--RGEELWYNEVFGFTFSNNTCSLK----SCGHYTQVVWADSSELGCGLAKC 346 R +A G + +P AS+M +M W+A A AQ Y +KC + G +G+N+ AT + + F G W E + +S+NT S+ GH TQ+ WA ++ +GCG+ C Sbjct: 35 RSRIAKGTYVAKGTAKPAASDMLKMKWDATVAASAQAYANKC--------PTGHSGAAGLGENLYWYWTSATITNIDQFGATGSAAWEKEFQDYGWSSNTLSMSLFNTGIGHATQMAWAKTNLIGCGVKNC 157
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG8483 (gene:FBgn0038126 transcript:FBtr0082782) HSP 1 Score: 74.3294 bits (181), Expect = 1.172e-16 Identity = 45/122 (36.89%), Postives = 71/122 (58.20%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE--------ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 R+ VA GR+PGQP A NM+++VW+ + A +AQ + D C F +D R F ++GQN+A + + W+NEV ++F + S K+ GHY+Q+VW ++S +GCG A+ Sbjct: 48 RQIVATGRYPGQPGAENMREIVWDDELAARAQKWADNCQFRHD--PHRTINRF-TMGQNLAIIWSTAPLDADDGDFPSRIQSWFNEVQKYSF-GDAWSPKT-GHYSQLVWGETSLVGCGYAE 164
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG16995 (gene:FBgn0031412 transcript:FBtr0077758) HSP 1 Score: 44.669 bits (104), Expect = 1.818e-6 Identity = 26/80 (32.50%), Postives = 45/80 (56.25%), Query Frame = 2 Query: 116 IFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEEL--WYNEVFGFTFSNNTCSLK-SCGHYTQVVWADSSELGCGLAKC 346 + + + + R+ G+ G+N+ SG N+ + + WY E+ + + N+ S + + GH+TQVVW S+ELG G AK Sbjct: 43 LLSRNRMEHRQNSGY---GENIYMASGGNLKGADAVRSWYEEIRQYNW--NSPSFQGNTGHFTQVVWKSSTELGVGFAKS 117
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG4270 (gene:FBgn0031407 transcript:FBtr0077755) HSP 1 Score: 42.3578 bits (98), Expect = 9.411e-6 Identity = 16/42 (38.10%), Postives = 27/42 (64.29%), Query Frame = 2 Query: 218 ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 E+WY E+ + F N + + GH+TQ++W S E+G G+A+ Sbjct: 101 EMWYREINSYDF-NKAQFVPTAGHFTQLIWKSSVEMGSGVAR 141
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: glipr1a (GLI pathogenesis-related 1a [Source:ZFIN;Acc:ZDB-GENE-110309-2]) HSP 1 Score: 74.7146 bits (182), Expect = 4.332e-17 Identity = 42/115 (36.52%), Postives = 64/115 (55.65%), Query Frame = 2 Query: 23 RFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDT---KDDRKYGGFESVGQNV---ATYSGKNIFRGEELWYNEVFGFTFSNNTCS-LKSCGHYTQVVWADSSELGCGLAKC 346 R P A+NM+ M W+A A A+ + C+F ++ + R + F +VG+N+ A YS + W NE+ + ++NN C+ K CGHYTQVVWADS ++GC + C Sbjct: 43 RSSVSPTAANMRYMTWDAALAVTARAWARFCLFKHNIHLREAKRVHPTFTTVGENIWAGAPYSRFTVKSAVFSWVNELKDYNYNNNQCNDKKVCGHYTQVVWADSYKVGCAVQTC 157
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: glipr1a (GLI pathogenesis-related 1a [Source:ZFIN;Acc:ZDB-GENE-110309-2]) HSP 1 Score: 74.3294 bits (181), Expect = 7.469e-17 Identity = 41/110 (37.27%), Postives = 63/110 (57.27%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDT---KDDRKYGGFESVGQNV---ATYSGKNIFRGEELWYNEVFGFTFSNNTCS-LKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ M W+A A A+ + C+F ++ + R + F +VG+N+ A YS + W NE+ + ++NN C+ K CGHYTQVVWADS ++GC + C Sbjct: 56 PTAANMRYMTWDAALAVTARAWARFCLFKHNIHLREAKRVHPTFTTVGENIWAGAPYSRFTVKSAVFSWVNELKDYNYNNNQCNDKKVCGHYTQVVWADSYKVGCAVQTC 165
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275]) HSP 1 Score: 71.633 bits (174), Expect = 6.896e-16 Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSG--KNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + +CI+ + + + +GQN++ SG K+I + WY+E F++ + CS C HYTQ+VWA S+++GC + KC Sbjct: 82 PPAANMEYMVWDERLAKSAEFWASQCIW-----EHGPHHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPSR-CSGSVCTHYTQMVWAASNKIGCAIKKC 180
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275]) HSP 1 Score: 71.633 bits (174), Expect = 6.896e-16 Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSG--KNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + +CI+ + + + +GQN++ SG K+I + WY+E F++ + CS C HYTQ+VWA S+++GC + KC Sbjct: 82 PPAANMEYMVWDERLAKSAEFWASQCIW-----EHGPHHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPSR-CSGSVCTHYTQMVWAASNKIGCAIKKC 180
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: pi15a (peptidase inhibitor 15a [Source:NCBI gene;Acc:561978]) HSP 1 Score: 65.4698 bits (158), Expect = 1.612e-13 Identity = 37/114 (32.46%), Postives = 64/114 (56.14%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P ASNM+ MVW+ A A+ + CI+ + ++ ++ +GQN++ +G+ +I + + W++EV ++F N C LK C HYTQ+VWA S+++GC + C Sbjct: 85 PPASNMEYMVWDDTLAKTAEQWASTCIWEHGPRNLLRF-----LGQNLSVRTGRYRSILQLVKPWHDEVKDYSFPYPRDCNPRCPLKCYGPMCTHYTQMVWATSNKVGCAINTC 193
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: ENSXETT00000036788.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464547.1:593:5826:1 gene:ENSXETG00000015856.1 transcript:ENSXETT00000036788.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.855 bits (159), Expect = 2.283e-13 Identity = 37/111 (33.33%), Postives = 61/111 (54.95%), Query Frame = 2 Query: 23 RFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFSNNTCSL-KSCGHYTQVVWADSSELGCGLAKC 346 R +P AS+M ++ W++ A+ Y +KCI+ ++ DR GF +G+N+ SG ++ G + W+ E + F+ C + CGHYTQ+VWA + +GCG C Sbjct: 46 RSQTEPSASDMIKLTWDSALEAMAKSYAEKCIWEHNK--DR---GF--IGENLFVMSGSSLDVALGLDDWHKERGYYNFTTGMCQEGQMCGHYTQMVWAGTERVGCGQNFC 149
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: pi15 (peptidase inhibitor 15 [Source:NCBI gene;Acc:100489915]) HSP 1 Score: 63.929 bits (154), Expect = 6.731e-13 Identity = 37/114 (32.46%), Postives = 61/114 (53.51%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSG--KNIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ + A A+ + CI+ + K+ +GQN++ +G K+I + + WY+EV + F N C L+ C HYTQ+VWA ++ +GC + C Sbjct: 83 PPAANMEYMVWDENLAKLAEAWAATCIWDHGPSYLLKF-----LGQNLSVRTGRYKSILQLVKPWYDEVKDYAFPYPQECNPRCPLRCYGPMCTHYTQMVWATTNRIGCAIHTC 191
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715]) HSP 1 Score: 62.003 bits (149), Expect = 3.398e-12 Identity = 35/114 (30.70%), Postives = 61/114 (53.51%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFSN---------NTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + ++C + + +Y +GQN++ +SG+ +I + WY+E ++F + N C+ C HYTQ+VWA S+ +GC + C Sbjct: 79 PPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRY-----IGQNLSVHSGRYRSIVDLVKGWYDERQHYSFPHPRECNPSCPNKCTGAVCTHYTQMVWASSNRIGCAVNIC 187
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715]) HSP 1 Score: 62.003 bits (149), Expect = 3.674e-12 Identity = 35/114 (30.70%), Postives = 61/114 (53.51%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFSN---------NTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + ++C + + +Y +GQN++ +SG+ +I + WY+E ++F + N C+ C HYTQ+VWA S+ +GC + C Sbjct: 74 PPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRY-----IGQNLSVHSGRYRSIVDLVKGWYDERQHYSFPHPRECNPSCPNKCTGAVCTHYTQMVWASSNRIGCAVNIC 182
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431]) HSP 1 Score: 62.003 bits (149), Expect = 6.310e-12 Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE----ELWYNEVFGFTFSNNT---------CSLKSCGHYTQVVWADSSELGCGLAKC 346 P ASNM+ M+W+ + A+ + + C++ + D +GQN+ + G+ +R + WY+EV +TF CS C HYTQ+VWA SS +GC + C Sbjct: 78 PPASNMEFMIWDVELERSAEAWAETCLWEHGPADL-----LPVIGQNLGAHWGR--YRPPTYHVQAWYDEVRDYTFPYPQECDPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLC 186
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Glipr1l1 (GLI pathogenesis-related 1 like 1 [Source:MGI Symbol;Acc:MGI:1916536]) HSP 1 Score: 72.4034 bits (176), Expect = 3.126e-16 Identity = 40/110 (36.36%), Postives = 64/110 (58.18%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFES---VGQNVATYSGKNIFRGEEL---WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 QP A++M Q+ W+ A A+ + +C A++ ++Y E +G+N+ Y G+ + E++ WYNE F F NTCS + CGHYTQVVWA + ++GC ++ C Sbjct: 57 QPPAADMNQLFWDQQLAKLAKAWTRECKLAHNPCIKQRYECLEDYDFIGENI--YLGRIETQPEDVVINWYNESKYFNFDFNTCS-EMCGHYTQVVWAKTVKIGCAVSNC 163
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Glipr1 (GLI pathogenesis-related 1 (glioma) [Source:MGI Symbol;Acc:MGI:1920940]) HSP 1 Score: 67.781 bits (164), Expect = 1.957e-14 Identity = 37/106 (34.91%), Postives = 55/106 (51.89%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTK-DDRKYGGFESVGQNV--ATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A NM M W+ A A+ + C F ++ + R + F ++G+N+ + S ++ WY E+ + FS C CGHYTQVVWADS +LGC + C Sbjct: 50 PPARNMLYMSWDPKLAQIAKAWTKSCEFKHNPQLHSRIHPNFTALGENIWLGSLSIFSVSSAISAWYEEIKHYDFSTRKCR-HVCGHYTQVVWADSYKLGCAVQLC 154
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Glipr1 (GLI pathogenesis-related 1 (glioma) [Source:MGI Symbol;Acc:MGI:1920940]) HSP 1 Score: 67.781 bits (164), Expect = 1.957e-14 Identity = 37/106 (34.91%), Postives = 55/106 (51.89%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTK-DDRKYGGFESVGQNV--ATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A NM M W+ A A+ + C F ++ + R + F ++G+N+ + S ++ WY E+ + FS C CGHYTQVVWADS +LGC + C Sbjct: 50 PPARNMLYMSWDPKLAQIAKAWTKSCEFKHNPQLHSRIHPNFTALGENIWLGSLSIFSVSSAISAWYEEIKHYDFSTRKCR-HVCGHYTQVVWADSYKLGCAVQLC 154
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Glipr1l3 (GLI pathogenesis-related 1 like 3 [Source:MGI Symbol;Acc:MGI:3620621]) HSP 1 Score: 66.6254 bits (161), Expect = 4.477e-14 Identity = 36/110 (32.73%), Postives = 65/110 (59.09%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYG---GFESVGQNVATYSGKNIFRGEEL---WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 QP A++M Q++W+ A A+ + +C ++ ++YG ++ +G+N+ Y G+ + E++ WYNE + F +NTCS K C +YTQV WA + ++GC ++ C Sbjct: 57 QPPAADMNQVIWDQKLAKLAKAWTRECKLGHNPCTSKQYGCLLDYDFIGENI--YLGEIETQPEDVVNNWYNENTDYNFVDNTCS-KICRNYTQVFWAKTFKIGCAVSNC 163
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: R3hdml (R3H domain containing-like [Source:MGI Symbol;Acc:MGI:3650937]) HSP 1 Score: 65.0846 bits (157), Expect = 1.747e-13 Identity = 38/115 (33.04%), Postives = 56/115 (48.70%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFSNN---------TCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + +CI+ + KY VGQN++ +SG+ ++ W E ++F CS C HYTQ+VWA SS LGC + C Sbjct: 78 HPPAANMEYMVWDEQLARSAEAWATQCIWTHGPSQLMKY-----VGQNLSIHSGRFRSVVDLVRSWSEEKRHYSFPAPKDCTPHCPWLCSGPVCSHYTQMVWASSSRLGCAINTC 187
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P35786|VA5_VESSQ (Venom allergen 5 OS=Vespula squamosa OX=30214 PE=1 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 1.272e-16 Identity = 49/114 (42.98%), Postives = 63/114 (55.26%), Query Frame = 2 Query: 29 PG-QPKASNMQQMVWNADSAPKAQIYPDKCIFANDT-KDDRKYGGFESVGQNVATYSG-----KNIFRGEELWYNEV--FGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 PG QP A NM +VWN + A AQI+ +C + +DT KD KY +VGQN+A S +N+ + W NEV F T S K GHYTQ+VWA + E+GCG K Sbjct: 66 PGPQPPAKNMNNLVWNNELANIAQIWASQCKYGHDTCKDTTKY----NVGQNIAVSSSTAAVYENVGNLVKAWENEVKDFNPTISWEQNEFKKIGHYTQMVWAKTKEIGCGSIK 175
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPU1|VA576_SCODE (Scoloptoxin SSD976 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 1.360e-16 Identity = 46/122 (37.70%), Postives = 66/122 (54.10%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK---NIFRGEELWYNEVFGFT------FSNNTCSLKSCGHYTQVVWADSSELGCGLA 340 R+ VA G+ GQP A+NM+++ WN + A AQ + C+F + K RK + +G+N+ Y G I R WY+EV T FS+ + GHYTQ+VWA++ LGCGL Sbjct: 47 RQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENI--YKGSYPDPIPRSVNAWYDEVKDVTPAVVKSFSSGGPMI---GHYTQMVWANTEALGCGLV 163
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P35787|VA5_VESVI (Venom allergen 5 OS=Vespula vidua OX=30215 PE=1 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 1.098e-15 Identity = 48/116 (41.38%), Postives = 64/116 (55.17%), Query Frame = 2 Query: 29 PG-QPKASNMQQMVWNADSAPKAQIYPDKCIFANDT-KDDRKYGGFESVGQNVATYSGKNIF-----RGEELWYNEVFGFT----FSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 PG QP A NM +VWN + A AQ++ +C + +DT KD KY VGQN+A S + ++W NEV F +S N +LK GHYTQ+VWA + E+GCG K Sbjct: 67 PGPQPPAKNMNNLVWNDELANIAQVWASQCNYGHDTCKDTEKY----PVGQNIAKRSTTAALFDSPGKLVKMWENEVKDFNPNIEWSKN--NLKKTGHYTQMVWAKTKEIGCGSVK 176
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPV2|VA558_SCODE (Scoloptoxin SSD558 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 1.596e-15 Identity = 45/122 (36.89%), Postives = 65/122 (53.28%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK---NIFRGEELWYNEVFGFT------FSNNTCSLKSCGHYTQVVWADSSELGCGLA 340 R+ VA G+ GQP A+NM+++ WN + A AQ + C+F + K RK + +G+N+ Y G I R WY+EV T FS+ + GHYTQ+VWA++ L CGL Sbjct: 47 RQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENI--YKGSYPDPIPRSVNAWYDEVKDVTPAVVKSFSSGGPMI---GHYTQMVWANTEALDCGLV 163
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|Q9DAG6|GPRL1_MOUSE (GLIPR1-like protein 1 OS=Mus musculus OX=10090 GN=Glipr1l1 PE=1 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 2.187e-15 Identity = 40/110 (36.36%), Postives = 64/110 (58.18%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFES---VGQNVATYSGKNIFRGEEL---WYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 QP A++M Q+ W+ A A+ + +C A++ ++Y E +G+N+ Y G+ + E++ WYNE F F NTCS + CGHYTQVVWA + ++GC ++ C Sbjct: 57 QPPAADMNQLFWDQQLAKLAKAWTRECKLAHNPCIKQRYECLEDYDFIGENI--YLGRIETQPEDVVINWYNESKYFNFDFNTCS-EMCGHYTQVVWAKTVKIGCAVSNC 163
BLAST of Venom allergen 5 vs. TrEMBL
Match: I1ZI85 (GLI pathogenesis related-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 147.902 bits (372), Expect = 2.483e-43 Identity = 69/100 (69.00%), Postives = 80/100 (80.00%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQV 301 R +A G GQP A+NMQQMVWNA+SA KAQI DKCIFA+DT DDRKYG FE VGQNVA ++G++I RG LWYN+V GFTF N+ CSLK+CGHYTQV Sbjct: 31 RSNLAQGEVSGQPPATNMQQMVWNAESASKAQITADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQDISRGVTLWYNQVSGFTFENSACSLKTCGHYTQV 130
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A430Q0R4 (SCP domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0000409 PE=3 SV=1) HSP 1 Score: 108.227 bits (269), Expect = 9.453e-28 Identity = 50/115 (43.48%), Postives = 72/115 (62.61%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R++V YG+ GQP A +++ + WN + KAQI D+C +DT DDRK F+ VGQN A K+I G +LW +E + F TC + CGHYTQ+VW D++++GCG+ C Sbjct: 13 RQSVQYGQLVGQPIAVSIKPLKWNVELEAKAQILSDQCRVGHDTNDDRKIPAFQYVGQNWA--GAKDINTGFQLWLDEYKNYDFYTRTCRMGQCGHYTQLVWEDTTDVGCGVTNC 125
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A430QAR7 (SCP domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0003460 PE=3 SV=1) HSP 1 Score: 106.301 bits (264), Expect = 5.585e-27 Identity = 47/115 (40.87%), Postives = 71/115 (61.74%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R+AV YG PGQP+A +M+ + WN + KAQ + D+C +D + +RK G F VGQN A + G ++W+NE + +S+NTC C HYTQ+VW ++++ GCG+ C Sbjct: 13 RQAVKYGLIPGQPRAVHMRLLKWNTELEMKAQNFSDQCRLGHDKESERKIGNFTYVGQNRAL--TPTVLVGFKMWFNEFQNYNYSSNTCPKGQCTHYTQLVWEETTDFGCGVTVC 125
BLAST of Venom allergen 5 vs. TrEMBL
Match: G4VMD2 (Venom allergen-like (VAL) 5 protein OS=Schistosoma mansoni OX=6183 GN=Smp_120670 PE=3 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 1.660e-26 Identity = 46/115 (40.00%), Postives = 72/115 (62.61%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R+AV YG PGQP+A +M + WN + A KAQ + D+C +D ++RK GFE VGQN A + G ++W++E + ++ NTC C HYTQ++W ++++ GCG+ +C Sbjct: 13 RQAVKYGLIPGQPEARHMNLLKWNTELAKKAQNFSDQCQLGHDKDEERKIPGFEYVGQNWAL--TPRLLIGFKMWFDESQNYNYNTNTCINNQCTHYTQLIWENTTDFGCGVTEC 125
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A3Q0KKB6 (Venom allergen-like (VAL) 5 protein OS=Schistosoma mansoni OX=6183 PE=3 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 2.633e-26 Identity = 46/115 (40.00%), Postives = 72/115 (62.61%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R+AV YG PGQP+A +M + WN + A KAQ + D+C +D ++RK GFE VGQN A + G ++W++E + ++ NTC C HYTQ++W ++++ GCG+ +C Sbjct: 13 RQAVKYGLIPGQPEARHMNLLKWNTELAKKAQNFSDQCQLGHDKDEERKIPGFEYVGQNWAL--TPRLLIGFKMWFDESQNYNYNTNTCINNQCTHYTQLIWENTTDFGCGVTEC 125
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000042591.1 (GLI pathogenesis related 1 [Source:NCBI gene;Acc:111193446]) HSP 1 Score: 75.0998 bits (183), Expect = 3.270e-17 Identity = 39/107 (36.45%), Postives = 65/107 (60.75%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDT---KDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 QP ASNM+ M W+ A A+ + KC+F ++T ++ R + F SVG+N+ + ++ + W +EV +++S TC + CGHYTQVVWA++ ++GC + C Sbjct: 46 QPAASNMRYMTWDEGLAVTARAWARKCLFIHNTHLKEEGRVHPVFSSVGENLWAGTHFSVQGAIKSWADEVKDYSYSTMTCK-QVCGHYTQVVWANTYKVGCAVQYC 151
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: pi15a (peptidase inhibitor 15-A-like [Source:NCBI gene;Acc:103031459]) HSP 1 Score: 68.1662 bits (165), Expect = 1.107e-14 Identity = 37/114 (32.46%), Postives = 65/114 (57.02%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P ASNM+ MVW+ A +A+ + C++ + ++ ++ +GQN++ +G+ +I + + WY+EV ++F N C LK C HYTQ+VWA S+++GC + C Sbjct: 84 PPASNMEYMVWDDSLAKRAEEWASTCLWEHGPRNLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYSFPYPRDCNPRCPLKCYGPMCTHYTQMVWATSNKIGCAIHTC 192
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: pi15a (peptidase inhibitor 15-A-like [Source:NCBI gene;Acc:103031459]) HSP 1 Score: 68.1662 bits (165), Expect = 1.188e-14 Identity = 37/114 (32.46%), Postives = 65/114 (57.02%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P ASNM+ MVW+ A +A+ + C++ + ++ ++ +GQN++ +G+ +I + + WY+EV ++F N C LK C HYTQ+VWA S+++GC + C Sbjct: 91 PPASNMEYMVWDDSLAKRAEEWASTCLWEHGPRNLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYSFPYPRDCNPRCPLKCYGPMCTHYTQMVWATSNKIGCAIHTC 199
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: r3hdml (R3H domain containing like [Source:NCBI gene;Acc:103046222]) HSP 1 Score: 67.3958 bits (163), Expect = 1.799e-14 Identity = 34/105 (32.38%), Postives = 59/105 (56.19%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ A A+ + +CI+ + KY +GQN++ SG+ ++ W++E F++ N C+ C HYTQ+VWA ++ +GC + +C Sbjct: 83 PPAANMEYMVWDERLAKSAESWASQCIWDHGPSHVMKY-----MGQNLSISSGRYRSVIELVRSWHDEKNSFSYPNR-CTGSVCSHYTQMVWASTNRIGCAINRC 181
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000036030.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846]) HSP 1 Score: 64.3142 bits (155), Expect = 2.819e-13 Identity = 36/112 (32.14%), Postives = 61/112 (54.46%), Query Frame = 2 Query: 17 YGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK-NIFRGEELWYNEVFGFTFSNNTCSL-KSCGHYTQVVWADSSELGCGLAKC 346 + R +P A++M M W+ + A A+ Y KCI+ ++ +G+N+ +G +I + W+ E +++ NNTC+ + CGHYTQ+VWA + E+GCG C Sbjct: 39 HHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQN------IMGENLFITTGPLDIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLC 144
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000374.1 (pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 gene:ENSPMAG00000000334.1 transcript:ENSPMAT00000000374.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 57.7658 bits (138), Expect = 2.538e-11 Identity = 31/115 (26.96%), Postives = 58/115 (50.43%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSG--KNIFRGEELWYNEVFGFTFSN---------NTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P+A++M+ M W+ + A A+ + +C + + D +++GQN+A Y G + + + W++E + + +TC C HYTQ+VWA+S +GC + C Sbjct: 21 HPQAADMEHMSWDWELAGMAEAWAKQCKWGHGPSDQ-----LDAIGQNLAVYVGSERPLAYHVQAWFDEEEWYQYPEEKECSPECPHTCRGPICTHYTQLVWAESRRVGCAVHTC 130
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: CRISP3 (cysteine rich secretory protein 3 [Source:HGNC Symbol;Acc:HGNC:16904]) HSP 1 Score: 56.6102 bits (135), Expect = 3.560e-11 Identity = 34/106 (32.08%), Postives = 54/106 (50.94%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFR-GEELWYNEVF--GFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A NM QM+W ++ A + + C F + + D R+ + G+N+ S + WYNEV GF + + + + GHYTQ+VW +S +LGC + C Sbjct: 60 PSAGNMLQMMWKKEAYLNALSWANNCTFGH-SPDARRKTTQWTCGENIFMSSAPFTWNYAIRDWYNEVISPGFQYDVGSTTTGAVGHYTQLVWYNSFQLGCAVNYC 164
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002250.1 (pep scaffold:Pmarinus_7.0:GL477299:1626:22672:-1 gene:ENSPMAG00000002049.1 transcript:ENSPMAT00000002250.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 43.8986 bits (102), Expect = 9.544e-7 Identity = 29/113 (25.66%), Postives = 47/113 (41.59%), Query Frame = 2 Query: 35 QPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P +SNM+ M W +A YP +C+ + + +GQ F + WY+E + + N C + C HYTQ+VWA ++ +GC + C Sbjct: 11 HPPSSNMEYMAWVKKLIKEAARYPGRCL-----RLMQPMAMIHFLGQPHWLSHRPPSFH-VQAWYDEAKDYQYPYEQECNPWCPFRCNGAVCTHYTQLVWATTNRVGCAINTC 117
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY2 (Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication [Source:SGD;Acc:S000001721]) HSP 1 Score: 50.447 bits (119), Expect = 5.563e-9 Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 2 Query: 218 ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + WYNE+ + +SN S +S GH+TQVVW +SE+GCGL C Sbjct: 258 DAWYNEITSYDYSNPGFS-ESAGHFTQVVWKGTSEVGCGLKSC 299
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY1 (Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication [Source:SGD;Acc:S000003615]) HSP 1 Score: 45.8246 bits (107), Expect = 1.755e-7 Identity = 19/43 (44.19%), Postives = 29/43 (67.44%), Query Frame = 2 Query: 218 ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + WYNE+ + FSN S + GH+TQVVW ++++GCG+ C Sbjct: 228 DAWYNEISNYDFSNPGFS-SNTGHFTQVVWKSTTQVGCGIKTC 269
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY3 (Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p [Source:SGD;Acc:S000003614]) HSP 1 Score: 44.669 bits (104), Expect = 5.358e-7 Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 Query: 218 ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 + WY E+ + +SN S +S GH+TQVVW ++E+GCG C Sbjct: 91 DAWYGEISKYNYSNPGFS-ESTGHFTQVVWKSTAEIGCGYKYC 132
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO49309 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH91]) HSP 1 Score: 52.7582 bits (125), Expect = 1.029e-9 Identity = 34/101 (33.66%), Postives = 51/101 (50.50%), Query Frame = 2 Query: 53 MQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEE---LWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 ++ + W+ D A AQ + +K K + G + G+N+A ++GK GEE +WY E + F N + GH+TQVVW + ELG G AK Sbjct: 58 VKNLKWSHDLARGAQAWAEKLARERTLKHSTRERGED--GENLAMFTGKYESAGEEATNMWYEESAYYNF-NRPGYQSNTGHFTQVVWKGTKELGLGRAKT 155
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO48881 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHM8]) HSP 1 Score: 50.8322 bits (120), Expect = 3.194e-9 Identity = 34/97 (35.05%), Postives = 51/97 (52.58%), Query Frame = 2 Query: 62 MVWNADSAPKAQIYPDKCIFANDTK--DDRKYGGFESVGQNVATYSGKNIF--RGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLA 340 + WNA A +AQ + + N + R+YG +++A SG + + ++WY EV + F N S S GH+TQVVWA S+E+G G A Sbjct: 22 LKWNARLASEAQSWAENLAQRNAIQHSSSREYG------ESIAYMSGAVLTGRKATDMWYGEVDKYRFENPGFST-SSGHFTQVVWAGSTEMGAGKA 111
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO37114 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGM4]) HSP 1 Score: 50.447 bits (119), Expect = 4.053e-9 Identity = 31/101 (30.69%), Postives = 52/101 (51.49%), Query Frame = 2 Query: 56 QQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKN-----IFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 ++M W+A A AQ + DK + K D +S G+N+A ++ + I + WY+E + ++N S K GH+TQ+VW + ++G G AK Sbjct: 19 KEMTWSASLAADAQKWADKLSQTDAFKHDYASINSKSQGENLAYFTPQKPNNTAIMDAFDAWYDESSKYDYANAKFS-KETGHFTQLVWKATQKMGIGTAK 118
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO48663 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI98]) HSP 1 Score: 49.2914 bits (116), Expect = 1.432e-8 Identity = 31/97 (31.96%), Postives = 51/97 (52.58%), Query Frame = 2 Query: 62 MVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE---ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAK 343 + W+ + A +AQ Y +K + K ++ G+N+A +SG+ G+ E+WY E ++F + + GH+TQ+VW S ELG G AK Sbjct: 23 LKWSDELAREAQYYAEKLAQQRSMQHSSKCSRNDA-GENLAMFSGRYDTAGDMACEMWYEESKKYSFVRGGSQMGT-GHFTQMVWKGSKELGMGRAK 117
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO40545 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6Z3]) HSP 1 Score: 47.7506 bits (112), Expect = 5.940e-8 Identity = 34/108 (31.48%), Postives = 51/108 (47.22%), Query Frame = 2 Query: 41 KASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVAT--------YSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLA 340 + N M N + + +A+ Y +K + E VG+N+A +GK WYNEV + F+N ++++ GH+TQVVW DS ELG G A Sbjct: 19 QVHNAPPMTLNRELSLQAKGYAEKIANLGALQHSSSGERGEDVGENLAVGCKSFGVPLTGKEAVTN---WYNEVCKYDFNNGAFAMET-GHFTQVVWTDSIELGMGKA 122
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020676.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728]) HSP 1 Score: 70.0922 bits (170), Expect = 1.553e-15 Identity = 40/116 (34.48%), Postives = 62/116 (53.45%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE-ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 RRAV +P A++M M ++ + A AQ + DKC+ + R G+E +G+N+ + +++ WY EV F F N + + K GHYTQVVW S ++GCG+ C Sbjct: 27 RRAV-------EPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGKPVGHYTQVVWNSSYKVGCGVTLC 134
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020674.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728]) HSP 1 Score: 70.0922 bits (170), Expect = 1.940e-15 Identity = 40/116 (34.48%), Postives = 62/116 (53.45%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE-ELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 RRAV +P A++M M ++ + A AQ + DKC+ + R G+E +G+N+ + +++ WY EV F F N + + K GHYTQVVW S ++GCG+ C Sbjct: 42 RRAV-------EPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGKPVGHYTQVVWNSSYKVGCGVTLC 149
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000023160.2 (peptidase inhibitor 16 [Source:NCBI gene;Acc:101164782]) HSP 1 Score: 68.5514 bits (166), Expect = 1.474e-14 Identity = 37/114 (32.46%), Postives = 62/114 (54.39%), Query Frame = 2 Query: 17 YGRFPGQ--PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK-NIFRGEELWYNEVFGFTFSNNTCSL-KSCGHYTQVVWADSSELGCGLAKC 346 + + GQ P A+ M + W+ + A+ Y KCI+ ++T+ E G+N+ +GK ++ E W+ E +++ NN+C K CGHYTQ+VWAD+ +GC + C Sbjct: 69 HNHYRGQVSPSAAAMLPLKWDGNLKLVAEGYAAKCIWEHNTE-------LEDTGENLFAGTGKLDLSEAVEKWFLERLDYSYENNSCDEDKMCGHYTQMVWADTHRVGCAVHFC 175
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: pi15a (peptidase inhibitor 15 [Source:NCBI gene;Acc:101173911]) HSP 1 Score: 64.3142 bits (155), Expect = 3.247e-13 Identity = 36/114 (31.58%), Postives = 62/114 (54.39%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGK--NIFRGEELWYNEVFGFTFS-----NNTCSLKS----CGHYTQVVWADSSELGCGLAKC 346 P ASNM+ MVW+ A A+ + C++ + ++ +GQN++ +G+ +I + + WY+EV ++F N C L+ C HYTQ+VWA S+++GC + C Sbjct: 82 PPASNMEYMVWDETLAKTAEDWAHACLWEHGPSHLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYSFPYPRDCNPRCPLRCYGPMCTHYTQMVWATSNKVGCAIHTC 190
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld2 (cysteine rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:101174090]) HSP 1 Score: 60.077 bits (144), Expect = 1.644e-11 Identity = 35/116 (30.17%), Postives = 59/116 (50.86%), Query Frame = 2 Query: 38 PKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGE----ELWYNEVFGFTFS---------NNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 P A+NM+ MVW+ + A + ++C + + +D S+GQN+A + G+ +R + WY+EV +T+ CS C HYTQ+VWA ++ +GC + C Sbjct: 92 PSAANMEHMVWDDELERSATQWAEECRWEHGPQD-----LLMSIGQNLAVHWGR--YRSPAFHVQAWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQLVWATTNRVGCAVHVC 200
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000016022.1 (SMESG000016022.1) HSP 1 Score: 196.823 bits (499), Expect = 2.071e-65 Identity = 94/115 (81.74%), Postives = 100/115 (86.96%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R VA G GQP A+NMQQMVWNA+SA KAQI DKCIFA+ TKDDRKYG FESVGQNVA +SGKNIFRG ELWYNE FGFTFSNNTCSLK+CGHYTQ+VWADSSELGCGLAKC Sbjct: 37 RGNVAQGVVSGQPPATNMQQMVWNAESASKAQISADKCIFAHGTKDDRKYGDFESVGQNVAMFSGKNIFRGVELWYNEAFGFTFSNNTCSLKTCGHYTQLVWADSSELGCGLAKC 151
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017383.1 (SMESG000017383.1) HSP 1 Score: 181.415 bits (459), Expect = 8.731e-60 Identity = 83/115 (72.17%), Postives = 97/115 (84.35%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R AVA G FPGQP A+NMQQMVWN +SA KAQIY DKCIFA+DT D+RKYG FE VGQNVA ++G+ I RG+ LWYNE GFTF N+ CSLKSCGHYTQ+VWA+S+E+GCGL+KC Sbjct: 37 RSAVAIGEFPGQPLATNMQQMVWNEESASKAQIYADKCIFAHDTIDNRKYGDFEWVGQNVAKFAGEIISRGDILWYNEASGFTFENSACSLKSCGHYTQLVWANSTEVGCGLSKC 151
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017386.1 (SMESG000017386.1) HSP 1 Score: 177.948 bits (450), Expect = 4.251e-58 Identity = 83/115 (72.17%), Postives = 96/115 (83.48%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R AV+ G GQP ASNMQQMVWNA+SA KAQI DKCIFA+DT DDRKYG FE VGQNVA ++G++I RG ELW+N+V GFTF N+ CSLK+CGHYTQ VWADS+ELGCGL+KC Sbjct: 37 RLAVSIGYASGQPSASNMQQMVWNAESASKAQITADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQDIARGAELWFNQVSGFTFENSACSLKTCGHYTQFVWADSTELGCGLSKC 151
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000015990.1 (SMESG000015990.1) HSP 1 Score: 170.629 bits (431), Expect = 2.506e-56 Identity = 81/95 (85.26%), Postives = 85/95 (89.47%), Query Frame = 2 Query: 62 MVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 MVWNA+SA KAQI DKCIFA+DTKDDRKY FESVGQNVA YS KNIFRG EL YNE FGFTFSNNTCSLK+CGHYTQ+VWADSSELGCGLAKC Sbjct: 1 MVWNAESASKAQISADKCIFAHDTKDDRKYEDFESVGQNVAMYSRKNIFRGVELLYNEAFGFTFSNNTCSLKTCGHYTQLVWADSSELGCGLAKC 95
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000000477.1 (SMESG000000477.1) HSP 1 Score: 172.555 bits (436), Expect = 6.180e-56 Identity = 80/115 (69.57%), Postives = 94/115 (81.74%), Query Frame = 2 Query: 2 RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYGGFESVGQNVATYSGKNIFRGEELWYNEVFGFTFSNNTCSLKSCGHYTQVVWADSSELGCGLAKC 346 R++VA G P QPKASNMQ+M WN SA KAQI DKCIFA+DTKDDRKYG FE VGQNVA ++ +NI RG ELW+N+ GFTF N+ CSLK+CGHYTQ+VWA+S+E GCGLAKC Sbjct: 37 RQSVAKGEVPNQPKASNMQKMDWNEKSASKAQITADKCIFAHDTKDDRKYGDFEWVGQNVAKFAEENIARGAELWFNQASGFTFENSACSLKTCGHYTQLVWANSTEFGCGLAKC 151 The following BLAST results are available for this feature:
BLAST of Venom allergen 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Venom allergen 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of Venom allergen 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Venom allergen 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Venom allergen 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026848 ID=SMED30026848|Name=Venom allergen 5|organism=Schmidtea mediterranea sexual|type=transcript|length=347bpback to top protein sequence of SMED30026848-orf-1 >SMED30026848-orf-1 ID=SMED30026848-orf-1|Name=SMED30026848-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp RRAVAYGRFPGQPKASNMQQMVWNADSAPKAQIYPDKCIFANDTKDDRKYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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