Cytochrome P450 1A1

Overview
NameCytochrome P450 1A1
Smed IDSMED30026830
Length (bp)792
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cytochrome P450 1A1 (SMED30026830) t-SNE clustered cells

Violin plots show distribution of expression levels for Cytochrome P450 1A1 (SMED30026830) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026830

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30026830h1SMcG0014314 dd_Smed_v6_20969_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Zebrafish
Match: cyp2ad2 (cytochrome P450, family 2, subfamily AD, polypeptide 2 [Source:ZFIN;Acc:ZDB-GENE-020812-2])

HSP 1 Score: 49.2914 bits (116), Expect = 2.479e-6
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 3 
Query:  189 FKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKE-FSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*ILH 536
            FK++++L K+YGK+FSL +    +I+++   ++K  LV + + F  R  + +   + K  G++  +G      R+  + +LRT       +E  I +EC  L +  K    EP N   ILH
Sbjct:   56 FKNINKLAKVYGKVFSLRVGSEKMIIVSGYKMVKEALVTQNDSFVLRPPVPLFHKVYKGIGLTMSNGYIWRSHRRFAASHLRTFGEGKKNLELGIQQECVYLCDAFKA-EKEPFNPIFILH 175
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Xenopus
Match: ENSXETT00000006615.1 (cytochrome P450 2K4-like [Source:NCBI gene;Acc:100493754])

HSP 1 Score: 48.1358 bits (113), Expect = 7.164e-6
Identity = 34/117 (29.06%), Postives = 55/117 (47.01%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVN-RKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKI----LSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNS 521
             +K+L  L K YG IFS+ +    ++V+     +K  L+N   +F+ R  L +   LAK HG+   +GE   + R+     L  +      ME  I EEC  L+    +   +P +S
Sbjct:   58 PYKTLLELSKTYGSIFSIQVGTEKMVVLCGYDTVKDALLNYPDDFADRPALPLIDDLAKRHGVFFSNGENWRVMRRFALSALKDFGMGKKRMEKTIIEECDHLVQKFNSYGGKPFDS 174
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Xenopus
Match: cyp2f1 (cytochrome P450 2K1 [Source:NCBI gene;Acc:100490476])

HSP 1 Score: 47.7506 bits (112), Expect = 9.620e-6
Identity = 34/123 (27.64%), Postives = 57/123 (46.34%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRK-EFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*ILHV 539
             F +   L + YG +FS+ +  + V+V+     +K  LVN   EFSGR  + +    +K HG+   DGE   + R+     LR        +E  I EE   L+   K+   +P ++  I++ 
Sbjct:   51 PFLTFMELAEKYGPVFSVQLGTKKVVVLCGTDAVKDALVNHADEFSGRPKIPMFDQTSKGHGVIFADGENWKVMRRFTLSTLRDFGMGKKTLEDRIGEESGCLVETFKSHEGKPFDNTMIMNA 173
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Mouse
Match: Cyp2f2 (cytochrome P450, family 2, subfamily f, polypeptide 2 [Source:MGI Symbol;Acc:MGI:88608])

HSP 1 Score: 50.447 bits (119), Expect = 1.029e-6
Identity = 38/118 (32.20%), Postives = 61/118 (51.69%), Query Frame = 3 
Query:  195 SLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*IL 533
            SL +L K YG +F++ +  R VIV++    +K  LV++ +EFSGR    V     + +GI+  DGE   I R+   + LR        +E  I EE S L+  ++ +  +P +   IL
Sbjct:   54 SLTKLSKEYGSVFTVYLGSRPVIVLSGYQTVKEALVDKGEEFSGRGAYPVFFNFTRGNGIAFSDGERWKILRRFSVQILRNFGMGKRSIEERILEEGSFLLEVLRKMEGKPFDPVFIL 171
[Close]
BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Match: sp|P20814|CP2CD_RAT (Cytochrome P450 2C13, male-specific OS=Rattus norvegicus OX=10116 GN=Cyp2c13 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.474e-6
Identity = 33/119 (27.73%), Postives = 55/119 (46.22%), Query Frame = 3 
Query:  192 KSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*IL 533
            +SL    K YG +++L +  +  +V++    +K  LV+  +EFSGR  L +   +AK  GI+   G      R    K LR        +E  + EE   L+  +K     PC+ + I+
Sbjct:   52 QSLTNFSKTYGPVYTLYVGSQPTVVLHGYEALKEALVDHGEEFSGRGRLPICEKVAKGQGIAFSHGNVWKATRHFTVKTLRNLGMGKGTIEDKVQEEAKWLVKELKKTNGSPCDPQFIM 170
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BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Match: sp|Q29510|CP2CU_RABIT (Cytochrome P450 2C30 OS=Oryctolagus cuniculus OX=9986 GN=CYP2C30 PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 3.870e-6
Identity = 35/119 (29.41%), Postives = 55/119 (46.22%), Query Frame = 3 
Query:  192 KSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*IL 533
            KS+  L K YG +F++    +  +V++    +K  LV+  +EFSGR T+ V    A+  G+    GE     R+     LR        +E  I EE S L+  +K     PC+   +L
Sbjct:   52 KSISMLAKEYGPVFTVYFGTKPSVVLHGYEAVKEALVDHGEEFSGRVTVPVFDRYAQGAGLFASQGELWKETRRFTLTVLRNMGMGKKTIEDRIQEEASCLVKALKETNASPCDPSSLL 170
[Close]
BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Match: sp|P33267|CP2F2_MOUSE (Cytochrome P450 2F2 OS=Mus musculus OX=10090 GN=Cyp2f2 PE=1 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 7.200e-6
Identity = 38/118 (32.20%), Postives = 61/118 (51.69%), Query Frame = 3 
Query:  195 SLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*IL 533
            SL +L K YG +F++ +  R VIV++    +K  LV++ +EFSGR    V     + +GI+  DGE   I R+   + LR        +E  I EE S L+  ++ +  +P +   IL
Sbjct:   54 SLTKLSKEYGSVFTVYLGSRPVIVLSGYQTVKEALVDKGEEFSGRGAYPVFFNFTRGNGIAFSDGERWKILRRFSVQILRNFGMGKRSIEERILEEGSFLLEVLRKMEGKPFDPVFIL 171
[Close]
BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Match: sp|O35293|CP2F2_RAT (Cytochrome P450 2F2 OS=Rattus norvegicus OX=10116 GN=Cyp2f2 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 8.328e-6
Identity = 38/118 (32.20%), Postives = 62/118 (52.54%), Query Frame = 3 
Query:  195 SLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLINFIKTITDEPCNSR*IL 533
            SL +L K YG +F++ +  R VIV++    +K  LV++ +EFSGR +  +     K +GI+  DGE   I R+   + LR        +E  I EE S L++ ++    +P +   IL
Sbjct:   54 SLTKLSKDYGSVFTVYLGPRRVIVLSGYQTVKEALVDKGEEFSGRGSYPIFFNFTKGNGIAFSDGERWKILRRFSVQILRNFGMGKRSIEERILEEGSFLLDVLRKTEGKPFDPVFIL 171
[Close]
BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Match: sp|P70687|CP17A_MESAU (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Mesocricetus auratus OX=10036 GN=CYP17A1 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 9.793e-6
Identity = 36/109 (33.03%), Postives = 59/109 (54.13%), Query Frame = 3 
Query:  204 RLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLA-KNHGISNYD-GEFVDIKRKI----LSKYLRTNDYMESIIDEECSLLINFIKTITDE 509
            +L + YG I+SL +   T ++I +  L K +LV + KEFSGR  +   GLL+ +  GI+  D G    + RK+     + +   N  +E II ++ S L +F+ T  +E
Sbjct:   55 KLQEKYGPIYSLRLGSTTTVIIGQYQLAKEVLVKKGKEFSGRPHMVTLGLLSDQGKGIAFADSGGSWQLHRKLALSSFALFRDGNQKLEKIICQKASSLCDFLLTHNEE 163
[Close]
BLAST of Cytochrome P450 1A1 vs. TrEMBL
Match: A0A5K3FXE9 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.675e-10
Identity = 40/128 (31.25%), Postives = 66/128 (51.56%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLR----------------TND---YMESIIDEECSLLINFIKTITDEP 512
            +FK L  L + YG + S  +CG+ VI +++   IK   + R+  +GR T+  + LLAK  GI+NYDGE     R+I  + L                 T+D    ++S ++ ECS +++F++    EP
Sbjct:   65 AFKELQVLRQKYGPVVSFRLCGKIVIGLSDEESIKEAALKRRNLTGRHTMLTNHLLAKGFGITNYDGENAAFSRRIFVRALNQFLPKGVRDIQAKDTPTDDANLQVDSDLNSECSKVVDFLRQKNGEP 192
[Close]
BLAST of Cytochrome P450 1A1 vs. TrEMBL
Match: A0A3P6GFM4 (SAM domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS4104 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 6.500e-10
Identity = 40/128 (31.25%), Postives = 66/128 (51.56%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLR----------------TND---YMESIIDEECSLLINFIKTITDEP 512
            +FK L  L + YG + S  +CG+ VI +++   IK   + R+  +GR T+  + LLAK  GI+NYDGE     R+I  + L                 T+D    ++S ++ ECS +++F++    EP
Sbjct:  199 AFKELQVLRQKYGPVVSFRLCGKIVIGLSDEESIKEAALKRRNLTGRHTMLTNHLLAKGFGITNYDGENAAFSRRIFVRALNQFLPKGVRDIQAKDTPTDDANLQVDSDLNSECSKVVDFLRQKNGEP 326
[Close]
BLAST of Cytochrome P450 1A1 vs. TrEMBL
Match: A0A0R3TC95 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS4682 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.474e-9
Identity = 40/124 (32.26%), Postives = 63/124 (50.81%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKIL---------------SKYLRTNDYMESIIDEECSLLINFIKTITDEP 512
            +FK + ++ K YG I S  +CG+ VIVI++ ++I+      +   GR     + +LAK +GISNYDGE     R+IL               S+ L  N  ++  +  EC  LI F+K +  +P
Sbjct:    6 AFKKVQQICKQYGPIISFRVCGKLVIVISDEAIIREAAFKTRMLIGRYPALTNHILAKRYGISNYDGENALNLRRILVRGLHRFLPNAISGISQPLCPNALVDDKLTVECDNLIRFLKQLHGKP 129
[Close]
BLAST of Cytochrome P450 1A1 vs. TrEMBL
Match: A0A158R6L1 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS428 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.829e-8
Identity = 37/138 (26.81%), Postives = 65/138 (47.10%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYL-----------------------------RTNDYMESIIDEECSLLINFIKTITDEP 512
            +F+ +  L + YG + S  ICG+ ++++++   IK   + R+   GR T+  + LLAK +GISNYDGE     R+IL   +                             +   +++  +D EC  L+N+++ I  EP
Sbjct:    7 AFEKVQLLCEKYGPVTSFRICGKLIVILSDEETIKEAALKRRTLIGRHTMLTNHLLAKGYGISNYDGENAYCLRRILVGAIHRILPTRTANAKSGNEVPSCDPSITQIECQNPSFIDDKLDTECLELVNYLRQIGGEP 144
[Close]
BLAST of Cytochrome P450 1A1 vs. TrEMBL
Match: A0A0R3U9U6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS3695 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.259e-8
Identity = 43/137 (31.39%), Postives = 63/137 (45.99%), Query Frame = 3 
Query:  186 SFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYL------RTNDYM----------------------ESIIDEECSLLINFIKTITDEP 512
            +FK +  L + YG + S  +CG+ VIV+N+   IK   + ++   GR T+  + LLAK  GISNYDGE     R+I  + L      RT D +                      E  +  ECS L++F+     EP
Sbjct:   65 AFKEVQLLHRKYGPVVSFRLCGKLVIVLNDEDSIKEAALRQRALIGRHTMLTNHLLAKGFGISNYDGENATFLRRIFIRGLHLVMPGRTCDRLGRSPPVSDDPLNASTDRADLLTEKKLAAECSKLVDFLHRQNGEP 201
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Nematostella
Match: EDO26422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TC22])

HSP 1 Score: 48.1358 bits (113), Expect = 5.474e-7
Identity = 31/98 (31.63%), Postives = 52/98 (53.06%), Query Frame = 3 
Query:  195 SLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRK-EFSGRTTLFVSGLLAKNHG---ISNYDGEFVDIKR---KILSKYLRTNDYMESIIDEE 467
            ++  L K YGKI+SLT+ G+  +V+N   L +  L+ RK EFSGR + FV   + ++      +++    V +++   K L  Y R    +E  I +E
Sbjct:   55 AMTELAKKYGKIYSLTLPGQQCVVLNSSELAREALLTRKDEFSGRPSTFVGDFITRSSADIICADHTQTMVRLRKIAHKALQMYGRGMQRLEGRICQE 152
[Close]
BLAST of Cytochrome P450 1A1 vs. Ensembl Nematostella
Match: EDO37670 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF10])

HSP 1 Score: 48.1358 bits (113), Expect = 1.566e-6
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3 
Query:  207 LGKIYGKIFSLTICGRTVIVINELSLIKHILVNRK-EFSGRTTLFVSGLLAKN 362
            L K YGKI+SLT+ G+  +V+N   L +  L+ RK EFSGR + FV   + ++
Sbjct:    4 LAKKYGKIYSLTLPGQQCVVLNSSELAREALLTRKDEFSGRPSTFVGDFITRS 56
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BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Match: SMESG000005914.1 (SMESG000005914.1)

HSP 1 Score: 238.81 bits (608), Expect = 6.723e-80
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 3 
Query:  105 KFRRASNXXXXXXXXXXXXXXXXXERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 524
            KFRRASNLPPGPIGLPFFGYLFFIERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR
Sbjct:   18 KFRRASNLPPGPIGLPFFGYLFFIERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 157
[Close]
BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Match: SMESG000005914.1 (SMESG000005914.1)

HSP 1 Score: 241.121 bits (614), Expect = 7.261e-77
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 3 
Query:  105 KFRRASNXXXXXXXXXXXXXXXXXERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 524
            KFRRASNLPPGPIGLPFFGYLFFIERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR
Sbjct:   18 KFRRASNLPPGPIGLPFFGYLFFIERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 157
[Close]
BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Match: SMESG000005914.1 (SMESG000005914.1)

HSP 1 Score: 234.572 bits (597), Expect = 5.324e-74
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:  177 ERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 524
            +RPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR
Sbjct:   68 KRPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTITDEPCNSR 183
[Close]
BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Match: SMESG000072744.1 (SMESG000072744.1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.463e-7
Identity = 36/127 (28.35%), Postives = 64/127 (50.39%), Query Frame = 3 
Query:  177 ERPSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNR-KEFSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRTNDYMES----IIDEECSLLINFIKTITDEPCNSR*ILHVF 542
            E P  K   RL K +G +F++    R VI++N+   +K   V+    +SGR  L  S L A N GI+  DG+    +R  + K L      +S    I+ +EC  L+  ++T+  +  ++  ++H++
Sbjct:   48 ETPILKYYERLIKKHGSVFAVRYGMRDVIILNDYQTVKSAFVDDGSNYSGRWRLVSSLLAANNSGIAFIDGQNWQTQRTFMLKVLHDFGMGKSVSYEIVRQECFNLLEELETLQGKRVDASDLIHLY 174
[Close]
BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Match: SMESG000072748.1 (SMESG000072748.1)

HSP 1 Score: 50.447 bits (119), Expect = 5.640e-7
Identity = 34/106 (32.08%), Postives = 50/106 (47.17%), Query Frame = 3 
Query:  183 PSFKSLHRLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKE-FSGRTTLFVSGLLAKNHGISNYDGEFVDIKRKILSKYLRT----NDYMESIIDEECSLLIN 485
            P +    +L K YG I+ +    R ++++N   L++  LVN  + FSGR+T      L  N GI   D     I+R  LSK LR     N     I   EC +L++
Sbjct:   50 PMYDFFMKLAKTYGPIYRIRFGMREMVILNNDDLVRKALVNNSDSFSGRSTTQKYTFLCMNSGIIFLDNHVWSIQRPFLSKVLRDFGMGNCKSVEIAQSECLMLLD 155
The following BLAST results are available for this feature:
BLAST of Cytochrome P450 1A1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cyp2ad22.479e-628.93cytochrome P450, family 2, subfamily AD, polypepti... [more]
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BLAST of Cytochrome P450 1A1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000006615.17.164e-629.06cytochrome P450 2K4-like [Source:NCBI gene;Acc:100... [more]
cyp2f19.620e-627.64cytochrome P450 2K1 [Source:NCBI gene;Acc:10049047... [more]
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BLAST of Cytochrome P450 1A1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Cyp2f21.029e-632.20cytochrome P450, family 2, subfamily f, polypeptid... [more]
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BLAST of Cytochrome P450 1A1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20814|CP2CD_RAT1.474e-627.73Cytochrome P450 2C13, male-specific OS=Rattus norv... [more]
sp|Q29510|CP2CU_RABIT3.870e-629.41Cytochrome P450 2C30 OS=Oryctolagus cuniculus OX=9... [more]
sp|P33267|CP2F2_MOUSE7.200e-632.20Cytochrome P450 2F2 OS=Mus musculus OX=10090 GN=Cy... [more]
sp|O35293|CP2F2_RAT8.328e-632.20Cytochrome P450 2F2 OS=Rattus norvegicus OX=10116 ... [more]
sp|P70687|CP17A_MESAU9.793e-633.03Steroid 17-alpha-hydroxylase/17,20 lyase OS=Mesocr... [more]
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BLAST of Cytochrome P450 1A1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3FXE92.675e-1031.25Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A3P6GFM46.500e-1031.25SAM domain-containing protein OS=Mesocestoides cor... [more]
A0A0R3TC952.474e-932.26Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A158R6L12.829e-826.81Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A0R3U9U63.259e-831.39Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Cytochrome P450 1A1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO264225.474e-731.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO376701.566e-643.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cytochrome P450 1A1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome P450 1A1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005914.16.723e-80100.00SMESG000005914.1[more]
SMESG000005914.17.261e-77100.00SMESG000005914.1[more]
SMESG000005914.15.324e-7499.14SMESG000005914.1[more]
SMESG000072744.12.463e-728.35SMESG000072744.1[more]
SMESG000072748.15.640e-732.08SMESG000072748.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026830 ID=SMED30026830|Name=Cytochrome P450 1A1|organism=Schmidtea mediterranea sexual|type=transcript|length=792bp
CTTTTAAATTTTACTATTTATATCTACTATGATCTTTCTTTAATATAGTA
TTTATGATATTTTTCACTTGTTTGTTTTGTTTAGTTTGTGTCTTTATATT
AACAAAATTCAGAAGGGCATCAAATTTGCCTCCAGGTCCCATAGGACTGC
CATTTTTTGGATATTTATTTTTCATTGAAAGACCATCATTTAAATCACTT
CACAGATTGGGGAAAATATACGGGAAAATATTTTCTCTCACAATTTGTGG
ACGAACAGTCATCGTTATAAACGAACTTTCACTTATAAAACATATTTTGG
TAAACAGAAAAGAATTTTCAGGCAGAACGACCTTATTTGTGAGCGGACTT
TTAGCCAAAAATCATGGCATTTCAAATTATGATGGGGAATTCGTTGATAT
TAAACGGAAGATATTAAGCAAATATCTTCGAACAAATGATTACATGGAAA
GTATCATTGATGAAGAATGCAGTCTTTTAATTAATTTTATCAAAACCATT
ACAGATGAGCCTTGTAACTCTCGGTAAATTTTGCATGTATTTAATTCAAC
CCCTCCCAAATTTCATCTAATATGTTAAAATAACTAATTACAATAGTATC
CGAACTGGCGGTCTTGTTATGTTCTAAAAAGTAAAACTTTAAACAAAACA
GAAATACACAGGGATATTTCAAAGTATGATGAATTGAGACCCCAAGGTAA
CCCTGTTTTGTTGAGAATTTTACTTTTCAAGAACATATATAGTTTCAAAC
GAAGTAATATTGTAATTAATTAGCCATTTCTTTTCCTTCAAC
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protein sequence of SMED30026830-orf-1

>SMED30026830-orf-1 ID=SMED30026830-orf-1|Name=SMED30026830-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp
MIFFTCLFCLVCVFILTKFRRASNLPPGPIGLPFFGYLFFIERPSFKSLH
RLGKIYGKIFSLTICGRTVIVINELSLIKHILVNRKEFSGRTTLFVSGLL
AKNHGISNYDGEFVDIKRKILSKYLRTNDYMESIIDEECSLLINFIKTIT
DEPCNSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036396Cyt_P450_sf
IPR001128Cyt_P450
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037heme binding
GO:0005506iron ion binding
GO:0004497monooxygenase activity
GO:0016491oxidoreductase activity
GO:0046872metal ion binding
GO:0070330aromatase activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 26..153
e-value: 2.2E-14
score: 53.1
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 17..157
e-value: 1.7E-24
score: 88.5
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 19..149
NoneNo IPR availablePANTHERPTHR24299:SF2coord: 6..151
NoneNo IPR availablePANTHERPTHR24299FAMILY NOT NAMEDcoord: 6..151
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.793