DUF3421 domain-containing protein

Overview
NameDUF3421 domain-containing protein
Smed IDSMED30026811
Length (bp)699
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DUF3421 domain-containing protein (SMED30026811) t-SNE clustered cells

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30026811) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DUF3421 domain-containing protein (SMED30026811) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30026811) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026811

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30026811h1SMcG0007834 SmedASXL_005847SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30026811h1SMcG0007834 Contig31307uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026811h1SMcG0007834 Contig31307newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026811h1SMcG0007834 Contig30508newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026811h1SMcG0007834 Contig30508uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30026811h1SMcG0007834 dd_Smed_v4_766_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026811h1SMcG0007834 dd_Smed_v4_766_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30026811h1SMcG0007834 dd_Smed_v4_766_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30026811h1SMcG0007834 dd_Smed_v4_766_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30026811h1SMcG0007834 Contig30508newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30026811h1SMcG0007834 Contig30508uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30026811h1SMcG0007834 dd_Smed_v6_766_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30026811h1SMcG0007834 dd_Smed_v6_766_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0345371)

HSP 1 Score: 91.2781 bits (225), Expect = 9.982e-22
Identity = 55/137 (40.15%), Postives = 71/137 (51.82%), Query Frame = 1 
Query:  205 GEIPHRTVKAG-----GKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAI-VGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            G +P   + AG        FVGRA  NG+ +P K+  G +  YV +GG+EI  +++E LV       D F W    G +VP  AI VG T  G+PLY+ R    G    GK  P H C Y P+GG+EHR    EVL 
Sbjct:   13 GSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV------GDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 2 Score: 77.7962 bits (190), Expect = 9.118e-17
Identity = 52/167 (31.14%), Postives = 79/167 (47.31%), Query Frame = 1 
Query:  115 IRWSYEEMSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGK-----CFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTD-NGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            + W  +E+SK   +   G     SW  +S G +P   ++ G        +VGR    G   PGK++  H+ +Y+ YGG+E  +  YE LV+          W    G  +  G +VGG D +G  +Y+ R+   G+ +  K  P  GCAY P+GG E  K   E+L 
Sbjct:   56 VPWGGQEISKHDFEVLVGDHF--SWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQPET-------WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 3 Score: 72.4034 bits (176), Expect = 8.700e-15
Identity = 45/122 (36.89%), Postives = 61/122 (50.00%), Query Frame = 1 
Query:  235 GGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIV-GGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            G + +VGRA   G  +P K+       YV YGG E+  ++YE L         G+ W +    NVP  A++ G T +G+PL+I R+  +G    GK    H C Y PF GEE R    EVL 
Sbjct:  169 GDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA------GYGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 4 Score: 48.1358 bits (113), Expect = 2.305e-6
Identity = 22/67 (32.84%), Postives = 38/67 (56.72%), Query Frame = 1 
Query:  187 WQKASNGEIPHRTV-----KAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVE 372
            W   S+G +P   V       G   F+GRA  +G   PGK+++ H  +Y+ + G+E+ +++YE LV+
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLVK 285
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0087792)

HSP 1 Score: 91.2781 bits (225), Expect = 9.982e-22
Identity = 55/137 (40.15%), Postives = 71/137 (51.82%), Query Frame = 1 
Query:  205 GEIPHRTVKAG-----GKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAI-VGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            G +P   + AG        FVGRA  NG+ +P K+  G +  YV +GG+EI  +++E LV       D F W    G +VP  AI VG T  G+PLY+ R    G    GK  P H C Y P+GG+EHR    EVL 
Sbjct:   13 GSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV------GDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 2 Score: 77.7962 bits (190), Expect = 9.118e-17
Identity = 52/167 (31.14%), Postives = 79/167 (47.31%), Query Frame = 1 
Query:  115 IRWSYEEMSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGK-----CFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTD-NGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            + W  +E+SK   +   G     SW  +S G +P   ++ G        +VGR    G   PGK++  H+ +Y+ YGG+E  +  YE LV+          W    G  +  G +VGG D +G  +Y+ R+   G+ +  K  P  GCAY P+GG E  K   E+L 
Sbjct:   56 VPWGGQEISKHDFEVLVGDHF--SWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQPET-------WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 3 Score: 72.4034 bits (176), Expect = 8.700e-15
Identity = 45/122 (36.89%), Postives = 61/122 (50.00%), Query Frame = 1 
Query:  235 GGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIV-GGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            G + +VGRA   G  +P K+       YV YGG E+  ++YE L         G+ W +    NVP  A++ G T +G+PL+I R+  +G    GK    H C Y PF GEE R    EVL 
Sbjct:  169 GDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA------GYGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 4 Score: 48.1358 bits (113), Expect = 2.305e-6
Identity = 22/67 (32.84%), Postives = 38/67 (56.72%), Query Frame = 1 
Query:  187 WQKASNGEIPHRTV-----KAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVE 372
            W   S+G +P   V       G   F+GRA  +G   PGK+++ H  +Y+ + G+E+ +++YE LV+
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLVK 285
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG10527 (gene:FBgn0034583 transcript:FBtr0071541)

HSP 1 Score: 91.6633 bits (226), Expect = 1.017e-21
Identity = 54/132 (40.91%), Postives = 72/132 (54.55%), Query Frame = 1 
Query:  187 WQKASNGEIPHRTVKAG----GKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGG-TDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEE 567
            W  A+NGE+P   ++ G     + ++ RA+  G  IPGKL+  H   YV++GG E     YE      VL   G QW  VD  N+P  A+  G T  G+PL+I R+  +G   VGK  P HGC Y P+GGEE
Sbjct:  159 WVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYE------VLCAGGGQWLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCYIPYGGEE 284

HSP 2 Score: 50.447 bits (119), Expect = 3.501e-7
Identity = 27/66 (40.91%), Postives = 34/66 (51.52%), Query Frame = 1 
Query:  403 WKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCC 600
            W       VP  A+ GG D+ + LYIAR+   G+ + GK  P HG  Y  +GG EH     EVLC 
Sbjct:  159 WVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLCA 224
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0084989)

HSP 1 Score: 77.7962 bits (190), Expect = 7.552e-18
Identity = 51/144 (35.42%), Postives = 66/144 (45.83%), Query Frame = 1 
Query:  184 SWQKASNGEIPHRTVKAG-----GKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAI-VGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            SW   SNG IP   V AG        F+GRA      +P K+       YV+Y  +E+ + NYE L   N      ++W   +   VP GA+ VG   +G+ LY  R    G   VGK  P HGC Y P+  EE +    EVL 
Sbjct:    5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVLSGFN------YEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVLS 142
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0307341)

HSP 1 Score: 77.7962 bits (190), Expect = 7.552e-18
Identity = 51/144 (35.42%), Postives = 66/144 (45.83%), Query Frame = 1 
Query:  184 SWQKASNGEIPHRTVKAG-----GKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAI-VGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLC 597
            SW   SNG IP   V AG        F+GRA      +P K+       YV+Y  +E+ + NYE L   N      ++W   +   VP GA+ VG   +G+ LY  R    G   VGK  P HGC Y P+  EE +    EVL 
Sbjct:    5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVLSGFN------YEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVLS 142
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H8B2 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012514g1 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 7.747e-39
Identity = 70/147 (47.62%), Postives = 95/147 (64.63%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHND-GFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHR 573
            MS+V+    +G+E+C+SW  ASNG+IP   VKAG  C+V RA+  G+++PGKL  GH   YV YG +E+  + YE LV + + H   G+QW    GN+VP GA+V G DNG PLY+AR+   GE   GK  P   CAY  +GG+EH+
Sbjct:    1 MSRVNHNDIEGIELCLSWHPASNGDIPAGAVKAGEDCYVARAKHEGEWVPGKLSAGHTSAYVPYGDEEVPYSEYEVLVSSEIGHFGCGYQWLPAKGNSVPKGALVSGYDNGAPLYVARATFEGEVCCGKAQPDLECAYISWGGKEHK 147
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8J9Y8 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.039e-37
Identity = 74/155 (47.74%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNV-LHNDGFQWKYVDGNNVPSGAIVGGTD-NGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVL 594
            MSKV     KG+EICVSWQ A +G IP   ++A   CFVGRA+    +IP K+ EGH   YV Y GKE  +  YE LV + +  +  G++W   D  +VP  A+V G + N +PLY+AR EV+GE  VGK    H CAYFP+GG EH     EVL
Sbjct:    1 MSKVKHAGHKGIEICVSWQPAKDGHIPPNAIEAAPGCFVGRAKHEDCWIPAKVAEGHTNAYVPYDGKEHSIPEYEVLVCSEIGCYGKGYKWVRKDSGDVPKKALVAGREKNDEPLYVARGEVDGEMCVGKVHKNHHCAYFPYGGTEHSSEEYEVL 155
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8HE36 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001642g3 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.097e-37
Identity = 74/155 (47.74%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNV-LHNDGFQWKYVDGNNVPSGAIVGGTD-NGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVL 594
            MSKV     KG+EICVSWQ A +G IP   ++A   CFVGRA+    +IP K+ EGH   YV Y GKE  +  YE LV + +  +  G++W   D  +VP  A+V G + N +PLY+AR EV+GE  VGK    H CAYFP+GG EH     EVL
Sbjct:    1 MSKVKHAGHKGIEICVSWQPAKDGHIPPNAIEAAPGCFVGRAKHEDCWIPAKVAEGHTNAYVPYDGKEHSIPEYEVLVCSEIGCYGKGYKWVREDSGDVPKKALVAGREKNDEPLYVARGEVDGEMCVGKVHKNHHCAYFPYGGTEHSSEEYEVL 155
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8I9L7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020479g1 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.156e-36
Identity = 70/163 (42.94%), Postives = 94/163 (57.67%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVET---NVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRK-TGVEVLCCVAKS 612
            MS   S  GKGV++C+SW  A+NGEIP   V  GG  FV R   +G+ +PGK+  GH   Y  Y G+E C + Y+ L ET    +    GF+W    GN  P  A++ G+D G+PLY+A++ ++GE   GKY P +G AYF  GG EH K    EVL    +S
Sbjct:    1 MSTFKSSGGKGVDLCLSWVNAANGEIPAGAVSIGGDVFVARCFSDGELLPGKVAAGHNSCYAPYDGEEKCCDTYQVLCETCNHGLTGGPGFEWVAWSGNTAPKHALISGSDQGRPLYVAKANIDGEWCAGKYIPDYGKAYFSCGGAEHEKEDDFEVLVMCTRS 163
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H2V3 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.759e-36
Identity = 67/143 (46.85%), Postives = 94/143 (65.73%), Query Frame = 1 
Query:  172 EICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNV-LHNDGFQWKYVDGNNVPSGAIVGG-TDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVL 594
            ++ +SW  A  G+IP+  ++ G   FV RAQ  G+ IPGK   GH+  YVSYGG+E  V++YE L +T++      + W+   G +VP GA++GG + + QP YI RS++NGE V GK F PHGCAYFP+GG+EHR    E+L
Sbjct:   20 QVTLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHQAAYVSYGGQEHAVSDYEILCDTHMQCFGCNYTWERCHGRSVPRGALIGGYSADRQPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEIL 162
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074377.1 (SMESG000074377.1)

HSP 1 Score: 327.02 bits (837), Expect = 2.105e-115
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAKSA 615
            MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAKSA
Sbjct:    1 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAKSA 160
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074396.1 (SMESG000074396.1)

HSP 1 Score: 191.43 bits (485), Expect = 7.522e-62
Identity = 92/159 (57.86%), Postives = 119/159 (74.84%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVL-HNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 609
            MSKVSSQYG GVE+ +SW KA+NG IP + V+AGG+ FV RA+ +GQYIPGK  EG+  V+VS+GG E   N YE LVE+++L  + G++WK+ +G NVP GA+VGG ++G PLY+ARS +  + VVGKY+P HGCAY P+GGEEHR    EVLC   K
Sbjct:    1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 159
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074378.1 (SMESG000074378.1)

HSP 1 Score: 191.43 bits (485), Expect = 7.522e-62
Identity = 92/159 (57.86%), Postives = 119/159 (74.84%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVL-HNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 609
            MSKVSSQYG GVE+ +SW KA+NG IP + V+AGG+ FV RA+ +GQYIPGK  EG+  V+VS+GG E   N YE LVE+++L  + G++WK+ +G NVP GA+VGG ++G PLY+ARS +  + VVGKY+P HGCAY P+GGEEHR    EVLC   K
Sbjct:    1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 159
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029910.1 (SMESG000029910.1)

HSP 1 Score: 185.267 bits (469), Expect = 1.792e-59
Identity = 86/158 (54.43%), Postives = 112/158 (70.89%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 609
            MSKV +QYG+ VEI VSWQ+A +G IP   VKA G CFV RA+ NG+YIPGKL  GH  V+V Y GKEI  ++Y+ LV+T++L   G++WK+ +   +P  AI+GGTDNGQPL+I +S V GE VVGK+ P HGCA+ P+ G E  K   E+LC + K
Sbjct:    1 MSKVITQYGRAVEISVSWQEAHDGSIPLHAVKADGSCFVARAKHNGEYIPGKLVTGHSHVFVCYDGKEITKSHYQVLVDTSILPTTGYEWKWSEDLEIPPNAIIGGTDNGQPLFIVKSIVEGETVVGKFHPDHGCAHVPYEGLEFEKNEFEILCSLKK 158
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029913.1 (SMESG000029913.1)

HSP 1 Score: 184.882 bits (468), Expect = 2.302e-59
Identity = 83/158 (52.53%), Postives = 109/158 (68.99%), Query Frame = 1 
Query:  136 MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 609
            MSK+ S+YG+ VEI VSW    +G IP + V AG  C+V RA+   +YIPGK  +GH   YVSY GKEI  + Y+ LV++++L     +W Y  GN +P  +IVGGTDN +PLYIA+S +NGE VVGKY P   C +FP+GG+EHRK+ VE+LCC  K
Sbjct:    1 MSKIVSEYGRSVEISVSWIDCQDGNIPDKAVSAGDSCYVARAKHLDEYIPGKFVQGHTSAYVSYDGKEIAKSEYQILVDSSILGGPSIEWHYTRGNQIPYRSIVGGTDNERPLYIAKSAINGEVVVGKYLPEFACGFFPYGGKEHRKSMVEILCCTTK 158
The following BLAST results are available for this feature:
BLAST of DUF3421 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG133219.982e-2240.15gene:FBgn0033787 transcript:FBtr0345371[more]
CG133219.982e-2240.15gene:FBgn0033787 transcript:FBtr0087792[more]
CG105271.017e-2140.91gene:FBgn0034583 transcript:FBtr0071541[more]
CG310867.552e-1835.42gene:FBgn0051086 transcript:FBtr0084989[more]
CG310867.552e-1835.42gene:FBgn0051086 transcript:FBtr0307341[more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8H8B27.747e-3947.62DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A1I8J9Y81.039e-3747.74DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A1I8HE361.097e-3747.74DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A1I8I9L71.156e-3642.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H2V32.759e-3646.85DUF3421 domain-containing protein OS=Macrostomum l... [more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000074377.12.105e-115100.00SMESG000074377.1[more]
SMESG000074396.17.522e-6257.86SMESG000074396.1[more]
SMESG000074378.17.522e-6257.86SMESG000074378.1[more]
SMESG000029910.11.792e-5954.43SMESG000029910.1[more]
SMESG000029913.12.302e-5952.53SMESG000029913.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026811 ID=SMED30026811|Name=DUF3421 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=699bp
GTTTGTCATTAAATAAATAATAATAATTCTCAGACTCAAATAACATAAAT
CTGTCATTTGTGCAGAAAATGTCAAATAAGTTATGTATTGAAATCAGTAT
ATAAAACAGTAAATATACGTTGGAGTTATGAAGAAATGAGCAAAGTTAGC
AGTCAATACGGTAAAGGAGTTGAGATTTGCGTTTCGTGGCAAAAGGCGTC
AAATGGTGAAATTCCTCATCGAACAGTTAAAGCGGGAGGCAAATGTTTCG
TAGGAAGAGCTCAACAGAACGGTCAATATATTCCAGGAAAACTCTACGAG
GGTCATAAAGGCGTTTATGTATCTTACGGTGGAAAAGAAATTTGCGTTAA
TAATTACGAAGCTTTGGTTGAAACAAATGTCTTGCATAATGATGGTTTCC
AATGGAAATATGTAGATGGAAATAATGTTCCGAGTGGTGCAATTGTTGGA
GGGACAGATAATGGACAACCTTTGTACATTGCAAGATCTGAAGTGAATGG
TGAGCCTGTAGTCGGAAAATATTTTCCTCCTCATGGATGTGCATACTTTC
CTTTTGGAGGCGAGGAGCATCGAAAAACTGGAGTTGAAGTGCTATGCTGT
GTTGCAAAGAGTGCTTAGAATTGAACTCTTAGTTTCTGGAACTTTTCTTT
ATTTATTTAATATATAATTACAAGCTTTTAAAAAAAAAAGATCGGAAGA
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protein sequence of SMED30026811-orf-1

>SMED30026811-orf-1 ID=SMED30026811-orf-1|Name=SMED30026811-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MSKVSSQYGKGVEICVSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIP
GKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDGFQWKYVDGNNVPS
GAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGV
EVLCCVAKSA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006368transcription elongation from RNA polymerase II promoter
GO:0016570histone modification
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0016593Cdc73/Paf1 complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000110pharynx progenitor cell
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR006616DM9_repeat
IPR024518DUF3421