RING domain ligase2 isoform 3
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026726 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16
Homology
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 93.5893 bits (231), Expect = 1.528e-18 Identity = 128/584 (21.92%), Postives = 232/584 (39.73%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ N K + K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L L+ H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I +++ NA+T S+ G + + L + N + + + LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD-----KKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLH-VGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 63.1586 bits (152), Expect = 3.034e-9 Identity = 29/93 (31.18%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK G TP A + T I +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKN--GYTPLHQAAQQGHTHIINVL 765 HSP 3 Score: 53.1434 bits (126), Expect = 3.512e-6 Identity = 26/101 (25.74%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H++ +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDA--AGKSGLTPLHVAAHY 591
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 92.8189 bits (229), Expect = 2.198e-18 Identity = 128/584 (21.92%), Postives = 232/584 (39.73%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ N K + K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L L+ H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I +++ NA+T S+ G + + L + N + + + LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 229 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD-----KKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPL-HVGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNN 780 HSP 2 Score: 85.8853 bits (211), Expect = 2.958e-16 Identity = 126/569 (22.14%), Postives = 214/569 (37.61%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKF------------HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
+HIA K LLL + N + E G+ P+H A GNI + LLL +D + +TP +A K + ++L ++ K + K GLT L A S +++++ +LDR P+ +K N L + + D L+ C+ ++ QH N + + L +AA GH + K + +K A + +N FT LH A N +++ +L H + + E+ T + +A + H + L + + NT N +T LH A SG + YL+ ++ T LH + G ++ +Q++ GA+ + + E +A L+++ +LSI +GKL + L S + +A + + LH+A ++ LLD GA NG +PL K N+M +I L+ YGAD N + I A++ E M++LL
Sbjct: 200 LHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARN--DITPLHVASKRGNANMVKLL----------------LDRGAK----IDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLN-----CV---QLLLQHNVPVDDVTNDYL----------TALHVAAHCGHYK-----VAKVLLDKKANPNA-----------------KALN--------GFTPLHIACKKNRIKVMELLLKH--GASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGH-------EDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLH----------IAAKKNQM-DIATTLLEYGADANAVTRQGIASVHLAAQ-EGHVDMVSLL 677 HSP 3 Score: 63.1586 bits (152), Expect = 2.907e-9 Identity = 29/93 (31.18%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK G TP A + T I +
Sbjct: 686 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKN--GYTPLHQAAQQGHTHIINVL 776 HSP 4 Score: 53.1434 bits (126), Expect = 2.915e-6 Identity = 26/101 (25.74%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H++ +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 504 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDA--AGKSGLTPLHVAAHY 602
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493]) HSP 1 Score: 92.0485 bits (227), Expect = 4.216e-18 Identity = 127/570 (22.28%), Postives = 220/570 (38.60%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP-------FEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
+HIA K LLL + N + + G+ P+H A GN+ + LLL +D + G+TP +A K T+ ++L L + K GLT L AA S D++++ +L+R P+ + N L + + D + EC+ + + QH A + + L +AA GH ++ K + +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V ++ GA+ + + + +N++ I E +I+ + S + H + LDK + + + G + LH+A + + + L GA D G +PL + C+ + +++ N L+ GA++N K+ N T A++ + I+ LL +
Sbjct: 175 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARN--GITPLHVASKRGNTNMVKL--------------------LLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHV-----ECVKH---LLQHKAPVDDVT----------LDYLTALHVAAHCGHYRVTKLLLDKRANPNARALRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLI-VACHYGN----------VKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHG 689 HSP 2 Score: 87.0409 bits (214), Expect = 1.368e-16 Identity = 119/523 (22.75%), Postives = 206/523 (39.39%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKL--------CKSYDFM----------SQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKL---SNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
G P+H+A ++G N+V LLL+ I+ + G P+H A +G+ ++V+LLL+ + RTK GL+P +A + +C+ ++L+ D + + L C Y + + G T L AA + ++++C+L N + D EE L S L K I+ ++ QH A +AA NG + L I+A +G + ++ ++ K + + A ++ + + +R +S K T LH A ++ + +L+ + + N T L +A + + L Y TN K TPLH A G D L+++ NI GLT LH A + NV + + +GA+ +L + L ++ + + FL K + NA+ N + LH A + + LL GAK + +NGN+ L
Sbjct: 237 GITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKN--GLSPLHMAAQGDHVECV-----KHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALRGETALHMAARAGQVEVVRCLL----RNGALVDARAR--EEQTPLHIASRLGKTEIV--QLLLQHMA----HPDAATTNG------YTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGY--TPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGY---------------------------TPLIVACHYGNVKMVNFLLKQGANV-NAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTAL 703 HSP 3 Score: 53.5286 bits (127), Expect = 2.176e-6 Identity = 29/101 (28.71%), Postives = 50/101 (49.50%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GKTE ++ L+ G+ P+HI+ +G +++ +LL G+ P+H A G++++ KLLLQ + D + G GLTP +A +
Sbjct: 413 GKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD--SAGKNGLTPLHVAAHY 511 HSP 4 Score: 52.7582 bits (125), Expect = 4.246e-6 Identity = 76/349 (21.78%), Postives = 126/349 (36.10%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQ-----------------------------QYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENE---ITIPFEKSIIALSLMENALT-LSIKFHGKLFLCF--------------LDKLNS--------------------TTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
FT L+ A +N D+VKY+L+ N + + E T LA+A H + + +L Q + + Q+K TPLH A G + L+NR +D G+T LH A +G N+ + +++ G C + + + E+ L+ +N L+ L + G C LD L + NAR + LHMA + Q E+ + LL +GA VD +PL + SR+ + EI +L+ + A + +TN T +++
Sbjct: 109 GFTPLYMAAQENHIDVVKYLLE--NGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPL-----HIASRLGK------TEIVQLLLQHMAHPDAATTNGYTPLHISAR 444 HSP 5 Score: 51.6026 bits (122), Expect = 9.564e-6 Identity = 26/78 (33.33%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKK 403
G+ P+ +AC G + +V LL + N+ + GY P+H A G+ I+ +LLQH + + T G T IAK+
Sbjct: 633 GYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTAN--GNTALAIAKR 708
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493]) HSP 1 Score: 91.6633 bits (226), Expect = 5.522e-18 Identity = 132/586 (22.53%), Postives = 229/586 (39.08%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIE-FEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP-------FEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E + L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V ++ GA+ + + + +N++ I E +I+ + S + H + LDK + + + G + LH+A + + + L GA D G +PL + C+ + +++ N L+ GA++N K+ N T A++ + I+ LL +
Sbjct: 210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLI-VACHYGN----------VKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHG 763 HSP 2 Score: 65.0846 bits (157), Expect = 7.084e-10 Identity = 108/485 (22.27%), Postives = 171/485 (35.26%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDING 2368
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L + LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+ N A V + +T L A R ++ ++ +NG + ++ A +LE+ +L K TPL VA + +D+ L+ D NG
Sbjct: 165 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTA-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR------------NGALVDARAREE------------------QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAH---SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNG 575 HSP 3 Score: 63.1586 bits (152), Expect = 2.992e-9 Identity = 46/163 (28.22%), Postives = 71/163 (43.56%), Query Frame = 2
Query: 152 KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF--HTENLIAAIKKN-DPIEFEKLCKSYDFMSQKLHT----------GLTILQTAADSNADKIIKCILDR 601
+ I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L H + AA P+ D S L G T L AA + + K +L R
Sbjct: 404 QAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREE--QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564 HSP 4 Score: 53.5286 bits (127), Expect = 2.515e-6 Identity = 29/101 (28.71%), Postives = 50/101 (49.50%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GKTE ++ L+ G+ P+HI+ +G +++ +LL G+ P+H A G++++ KLLLQ + D + G GLTP +A +
Sbjct: 487 GKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD--SAGKNGLTPLHVAAHY 585 HSP 5 Score: 51.6026 bits (122), Expect = 8.584e-6 Identity = 26/86 (30.23%), Postives = 43/86 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIE 427
G+ P+ +AC G + +V LL + N+ + GY P+H A G+ I+ +LLQH + + T G T IAK+ ++
Sbjct: 707 GYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTAN--GNTALAIAKRLGYISVVD 790
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493]) HSP 1 Score: 91.6633 bits (226), Expect = 6.145e-18 Identity = 132/586 (22.53%), Postives = 229/586 (39.08%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIE-FEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP-------FEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E + L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V ++ GA+ + + + +N++ I E +I+ + S + H + LDK + + + G + LH+A + + + L GA D G +PL + C+ + +++ N L+ GA++N K+ N T A++ + I+ LL +
Sbjct: 210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLI-VACHYGN----------VKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHG 763 HSP 2 Score: 63.929 bits (154), Expect = 1.542e-9 Identity = 109/489 (22.29%), Postives = 173/489 (35.38%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVL 2380
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L + LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+ N A V + +T L A R ++ ++ +NG + ++ A +LE+ +L K TPL VA + +D+ L+ D NG + L
Sbjct: 165 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTA-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLH-IACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR------------NGALVDARAREE------------------QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAH---SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPL 579 HSP 3 Score: 62.3882 bits (150), Expect = 5.129e-9 Identity = 46/163 (28.22%), Postives = 71/163 (43.56%), Query Frame = 2
Query: 152 KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF--HTENLIAAIKKN-DPIEFEKLCKSYDFMSQKLHT----------GLTILQTAADSNADKIIKCILDR 601
+ I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L H + AA P+ D S L G T L AA + + K +L R
Sbjct: 404 QAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREE--QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564 HSP 4 Score: 53.1434 bits (126), Expect = 3.311e-6 Identity = 29/101 (28.71%), Postives = 50/101 (49.50%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GKTE ++ L+ G+ P+HI+ +G +++ +LL G+ P+H A G++++ KLLLQ + D + G GLTP +A +
Sbjct: 487 GKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD--SAGKNGLTPLHVAAHY 585
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 73.1738 bits (178), Expect = 9.488e-13 Identity = 119/558 (21.33%), Postives = 223/558 (39.96%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL--DRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIF 1795
D KG P +HIA +K LLL N + G+ P+H A G+ + +LLL+ ++ + + ++P +A KW RT+ L + I D ++ L LT L AA S D+++ ++ P+ K N L + + D + + + + +R+ + + ++ A G H + L+ +A+ + ++G I K ++ + E + + E+ + T LH A ++V Y+L N D+++ T L LA + ++VL + + Q + QTPLH A G D L+ N + + LH A +G E V ++++ A+ + F S + + E+ +N +T L + H K+ + L+ N ++ A N + LH+A K Q E+ +LL A + + G +PL ++ ++ ++ EI+ +LI G+D+ K+ N +T
Sbjct: 188 DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQAR--HNISPLHVATKWGRTNMANLLLSRGAI-----------------IDSRTKDL---LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHV-DAARTLLYHRAPVDDVTVDYLTPLHVAAHCG-HVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVV------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE--NGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLH---------LSAQEGHK--EISGLLIENGSDVGAKANNGLTAM 693
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 73.1738 bits (178), Expect = 9.488e-13 Identity = 119/558 (21.33%), Postives = 223/558 (39.96%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL--DRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIF 1795
D KG P +HIA +K LLL N + G+ P+H A G+ + +LLL+ ++ + + ++P +A KW RT+ L + I D ++ L LT L AA S D+++ ++ P+ K N L + + D + + + + +R+ + + ++ A G H + L+ +A+ + ++G I K ++ + E + + E+ + T LH A ++V Y+L N D+++ T L LA + ++VL + + Q + QTPLH A G D L+ N + + LH A +G E V ++++ A+ + F S + + E+ +N +T L + H K+ + L+ N ++ A N + LH+A K Q E+ +LL A + + G +PL ++ ++ ++ EI+ +LI G+D+ K+ N +T
Sbjct: 188 DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQAR--HNISPLHVATKWGRTNMANLLLSRGAI-----------------IDSRTKDL---LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHV-DAARTLLYHRAPVDDVTVDYLTPLHVAAHCG-HVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVV------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE--NGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLH---------LSAQEGHK--EISGLLIENGSDVGAKANNGLTAM 693
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 73.1738 bits (178), Expect = 9.623e-13 Identity = 119/558 (21.33%), Postives = 223/558 (39.96%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL--DRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIF 1795
D KG P +HIA +K LLL N + G+ P+H A G+ + +LLL+ ++ + + ++P +A KW RT+ L + I D ++ L LT L AA S D+++ ++ P+ K N L + + D + + + + +R+ + + ++ A G H + L+ +A+ + ++G I K ++ + E + + E+ + T LH A ++V Y+L N D+++ T L LA + ++VL + + Q + QTPLH A G D L+ N + + LH A +G E V ++++ A+ + F S + + E+ +N +T L + H K+ + L+ N ++ A N + LH+A K Q E+ +LL A + + G +PL ++ ++ ++ EI+ +LI G+D+ K+ N +T
Sbjct: 188 DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQAR--HNISPLHVATKWGRTNMANLLLSRGAI-----------------IDSRTKDL---LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHV-DAARTLLYHRAPVDDVTVDYLTPLHVAAHCG-HVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVV------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE--NGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLH---------LSAQEGHK--EISGLLIENGSDVGAKANNGLTAM 693
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 73.1738 bits (178), Expect = 9.623e-13 Identity = 119/558 (21.33%), Postives = 223/558 (39.96%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL--DRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIF 1795
D KG P +HIA +K LLL N + G+ P+H A G+ + +LLL+ ++ + + ++P +A KW RT+ L + I D ++ L LT L AA S D+++ ++ P+ K N L + + D + + + + +R+ + + ++ A G H + L+ +A+ + ++G I K ++ + E + + E+ + T LH A ++V Y+L N D+++ T L LA + ++VL + + Q + QTPLH A G D L+ N + + LH A +G E V ++++ A+ + F S + + E+ +N +T L + H K+ + L+ N ++ A N + LH+A K Q E+ +LL A + + G +PL ++ ++ ++ EI+ +LI G+D+ K+ N +T
Sbjct: 188 DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQAR--HNISPLHVATKWGRTNMANLLLSRGAI-----------------IDSRTKDL---LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHV-DAARTLLYHRAPVDDVTVDYLTPLHVAAHCG-HVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVV------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE--NGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLH---------LSAQEGHK--EISGLLIENGSDVGAKANNGLTAM 693
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 73.1738 bits (178), Expect = 1.010e-12 Identity = 119/558 (21.33%), Postives = 223/558 (39.96%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL--DRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIF 1795
D KG P +HIA +K LLL N + G+ P+H A G+ + +LLL+ ++ + + ++P +A KW RT+ L + I D ++ L LT L AA S D+++ ++ P+ K N L + + D + + + + +R+ + + ++ A G H + L+ +A+ + ++G I K ++ + E + + E+ + T LH A ++V Y+L N D+++ T L LA + ++VL + + Q + QTPLH A G D L+ N + + LH A +G E V ++++ A+ + F S + + E+ +N +T L + H K+ + L+ N ++ A N + LH+A K Q E+ +LL A + + G +PL ++ ++ ++ EI+ +LI G+D+ K+ N +T
Sbjct: 188 DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQAR--HNISPLHVATKWGRTNMANLLLSRGAI-----------------IDSRTKDL---LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHV-DAARTLLYHRAPVDDVTVDYLTPLHVAAHCG-HVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVV------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE--NGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLH---------LSAQEGHK--EISGLLIENGSDVGAKANNGLTAM 693
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303119) HSP 1 Score: 89.7373 bits (221), Expect = 7.740e-18 Identity = 129/572 (22.55%), Postives = 228/572 (39.86%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQ-------SFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXX------EIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLEN 1819
+HIA +K + LLL+ + N + G+ P+H A+ GN I LL+Q ++ K ++P +A KW +T+ + L ++K IE K+ D GLT L AA S ++++ +L+R P+ K N L + +H A G + ++AA I H+ + L +AA GH +L +D N RA+ ++ E + + + T LH A ++V Y+L H + D+ + T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G + V ++ NGA + F + I + +K + +N +T L + H ++ L L+K S + A++G ++ LH+A + Q ++ +LL+ GA + + G +PL ++ EI+N+LI + A +N + N +T ++ +N
Sbjct: 176 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK--HNISPLHVAAKWGKTNMVSLL--------LEKGGNIE----AKTRD--------GLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGL----------------------APLHMAAQG--EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD-------RNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNG--HTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHA-----------EISNLLIEHKAAVNHPAKNGLTPMHLCAQEDN 679 HSP 2 Score: 62.3882 bits (150), Expect = 2.043e-9 Identity = 79/344 (22.97%), Postives = 140/344 (40.70%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEIT--IPFEKSIIALSLMENALTLSIKFH-GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFE---MFC-------------------NSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
++R KV LH A +D +LD+ +N D+ S + T L +A ++ +L Q N K++ +PLH A G + L+ + NI+ GLT LHCA G E V + ++ GA + L L Q + + + ++ + ++ L + H G + + L + +NAR N + LH+A K + ++ + LL GA + +G +PL M C + + +I IL+ GA ++ ++ + T AS+L N I++ LL + + ++KD A
Sbjct: 166 DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGF--TPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQV-DATTKDMYTAL 506 HSP 3 Score: 59.6918 bits (143), Expect = 1.514e-8 Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K L +V LLL +I G P+H A G + IV LLQH D T G TP +A + ++TD I +
Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPT--VRGETPLHLAARANQTDIIRIL 455 HSP 4 Score: 58.9214 bits (141), Expect = 2.394e-8 Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHT 439
G+ P+H+A G N+V LL N++ GY P+H G+ IV LLL+HK + +T G TP IA+K ++ T
Sbjct: 700 GYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVN--GQTPLHIARKLGYISVLDSLKT 787 HSP 5 Score: 57.7658 bits (138), Expect = 4.924e-8 Identity = 42/143 (29.37%), Postives = 66/143 (46.15%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDC--IELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR 601
P+H+A + G N+V LLL + NIE + G P+H A +G+ ++V +LL+ I +TK GL P +A + D I L+H P+ +++ Y LT L AA ++ K +LDR
Sbjct: 241 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKN--GLAPLHMAAQGEHVDAARILLYH----------RAPV--DEVTVDY----------LTALHVAAHCGHVRVAKLLLDR 359 HSP 6 Score: 55.0694 bits (131), Expect = 3.677e-7 Identity = 26/90 (28.89%), Postives = 46/90 (51.11%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ G P+H+A G +NIVI LL + + + + G P+H A +I+++LL++ ++D R + TP IA + D + L
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQ--QTPLHIASRLGNVDIVMLL 488 HSP 7 Score: 53.9138 bits (128), Expect = 8.587e-7 Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
GH P+HIA K ++I LL E G+ P+H ++ G+ EI LL++HK ++ K GLTP + + + E+
Sbjct: 601 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKN--GLTPMHLCAQEDNVNVAEIL 686 HSP 8 Score: 53.1434 bits (126), Expect = 1.490e-6 Identity = 33/104 (31.73%), Postives = 51/104 (49.04%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTEN---LIAAIKKN 469
+G P+H+A +I+ +LL ++ P+H A+ GN++IV LLLQH ++D TK + T IA K + + + EN L AA KK
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVL-IENGAALDAATKKG 535 HSP 9 Score: 50.8322 bits (120), Expect = 7.401e-6 Identity = 70/294 (23.81%), Postives = 111/294 (37.76%), Query Frame = 2
Query: 968 FTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSENEIT-IPFEKSIIALSLMENALTLSIKFHGKLFLCFL-DKLNSTTSNARDGNNNSL--------LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKL 1813
+T LH A + ++ +++ N LD+ + T L L H K ++L Q E + Q K TPLH A L+ + + G T LH A K ++ ++ GA N L + Q EI+ + E +N LT + LC D +N ++G N + LH+A Q + + LL +GA VD S G +PL + + I N+L+ + A+ N ++ N T A KL
Sbjct: 503 YTALHIAAKEGQDEVAAVLIE--NGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLT-------PMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGH-----------CHIVNLLLEHKANANAQTVNGQTPLHIARKL 776 HSP 10 Score: 50.447 bits (119), Expect = 9.384e-6 Identity = 79/360 (21.94%), Postives = 141/360 (39.17%), Query Frame = 2
Query: 788 LEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVN------FTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG--TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
L AA GHE +D ++++ I + E + L R V+ T LH A + K +LD + D+N L T L +A + K +++L ++ + + + TPLH A G + YL+ + D G T LH A ++ ++ NGA Q ++ E + P IA L + + + HG ++++TT + + LH+A K Q E+ L+++GA +D G +PL +T + + I++ +L+ AD++ + N +T A N+++ L LL
Sbjct: 275 LHCAARSGHEQVVDMLLER---GAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDR----NADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGA-----------QVDARAREQQ--TPLH---IASRLGNVDIVMLLLQHGA-------QVDATTKDMY-----TALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLH---------LTAKYGH--IKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLL 588
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334061) HSP 1 Score: 88.1965 bits (217), Expect = 2.575e-17 Identity = 129/572 (22.55%), Postives = 228/572 (39.86%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQ-------SFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXX------EIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLEN 1819
+HIA +K + LLL+ + N + G+ P+H A+ GN I LL+Q ++ K ++P +A KW +T+ + L ++K IE K+ D GLT L AA S ++++ +L+R P+ K N L + +H A G + ++AA I H+ + L +AA GH +L +D N RA+ ++ E + + + T LH A ++V Y+L H + D+ + T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G + V ++ NGA + F + I + +K + +N +T L + H ++ L L+K S + A++G ++ LH+A + Q ++ +LL+ GA + + G +PL ++ EI+N+LI + A +N + N +T ++ +N
Sbjct: 326 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK--HNISPLHVAAKWGKTNMVSLL--------LEKGGNIE----AKTRD--------GLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGL----------------------APLHMAAQG--EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD-------RNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNG--HTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHA-----------EISNLLIEHKAAVNHPAKNGLTPMHLCAQEDN 829 HSP 2 Score: 60.8474 bits (146), Expect = 5.830e-9 Identity = 79/344 (22.97%), Postives = 140/344 (40.70%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEIT--IPFEKSIIALSLMENALTLSIKFH-GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFE---MFC-------------------NSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
++R KV LH A +D +LD+ +N D+ S + T L +A ++ +L Q N K++ +PLH A G + L+ + NI+ GLT LHCA G E V + ++ GA + L L Q + + + ++ + ++ L + H G + + L + +NAR N + LH+A K + ++ + LL GA + +G +PL M C + + +I IL+ GA ++ ++ + T AS+L N I++ LL + + ++KD A
Sbjct: 316 DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGF--TPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQV-DATTKDMYTAL 656 HSP 3 Score: 59.3066 bits (142), Expect = 2.151e-8 Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K L +V LLL +I G P+H A G + IV LLQH D T G TP +A + ++TD I +
Sbjct: 520 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPT--VRGETPLHLAARANQTDIIRIL 605 HSP 4 Score: 58.5362 bits (140), Expect = 3.314e-8 Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHT 439
G+ P+H+A G N+V LL N++ GY P+H G+ IV LLL+HK + +T G TP IA+K ++ T
Sbjct: 850 GYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQT--VNGQTPLHIARKLGYISVLDSLKT 937 HSP 5 Score: 58.151 bits (139), Expect = 4.613e-8 Identity = 42/143 (29.37%), Postives = 66/143 (46.15%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDC--IELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR 601
P+H+A + G N+V LLL + NIE + G P+H A +G+ ++V +LL+ I +TK GL P +A + D I L+H P+ +++ Y LT L AA ++ K +LDR
Sbjct: 391 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKN--GLAPLHMAAQGEHVDAARILLYH----------RAPV--DEVTVDY----------LTALHVAAHCGHVRVAKLLLDR 509 HSP 6 Score: 54.6842 bits (130), Expect = 5.572e-7 Identity = 26/90 (28.89%), Postives = 46/90 (51.11%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ G P+H+A G +NIVI LL + + + + G P+H A +I+++LL++ ++D R + TP IA + D + L
Sbjct: 551 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQ--QTPLHIASRLGNVDIVMLL 638 HSP 7 Score: 53.9138 bits (128), Expect = 9.423e-7 Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
GH P+HIA K ++I LL E G+ P+H ++ G+ EI LL++HK ++ K GLTP + + + E+
Sbjct: 751 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKN--GLTPMHLCAQEDNVNVAEIL 836 HSP 8 Score: 52.7582 bits (125), Expect = 2.054e-6 Identity = 33/104 (31.73%), Postives = 51/104 (49.04%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTEN---LIAAIKKN 469
+G P+H+A +I+ +LL ++ P+H A+ GN++IV LLLQH ++D TK + T IA K + + + EN L AA KK
Sbjct: 585 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVL-IENGAALDAATKKG 685 HSP 9 Score: 50.8322 bits (120), Expect = 8.313e-6 Identity = 70/294 (23.81%), Postives = 111/294 (37.76%), Query Frame = 2
Query: 968 FTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSENEIT-IPFEKSIIALSLMENALTLSIKFHGKLFLCFL-DKLNSTTSNARDGNNNSL--------LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKL 1813
+T LH A + ++ +++ N LD+ + T L L H K ++L Q E + Q K TPLH A L+ + + G T LH A K ++ ++ GA N L + Q EI+ + E +N LT + LC D +N ++G N + LH+A Q + + LL +GA VD S G +PL + + I N+L+ + A+ N ++ N T A KL
Sbjct: 653 YTALHIAAKEGQDEVAAVLIE--NGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLT-------PMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGH-----------CHIVNLLLEHKANANAQTVNGQTPLHIARKL 926
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303118) HSP 1 Score: 87.8113 bits (216), Expect = 4.267e-17 Identity = 129/562 (22.95%), Postives = 225/562 (40.04%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQ-------SFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXX------XXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRIT 1789
+HIA +K + LLL+ + N + G+ P+H A+ GN I LL+Q ++ K ++P +A KW +T+ + L ++K IE K+ D GLT L AA S ++++ +L+R P+ K N L + +H A G + ++AA I H+ + L +AA GH +L +D N RA+ K ++ E + + + T LH A ++V Y+L H + D+ + T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G + V ++ NGA + F + I + +K + +N +T L + H ++ L L+K S + A++G ++ LH+A + Q ++ +LL+ GA + + G +PL ++ EI+N+LI + A +N + N +T
Sbjct: 176 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK--HNISPLHVAAKWGKTNMVSLL--------LEKGGNIE----AKTRD--------GLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGL----------------------APLHMAAQG--EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD-------RNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNG--HTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHA-----------EISNLLIEHKAAVNHPAKNGLT 669 HSP 2 Score: 61.2326 bits (147), Expect = 5.672e-9 Identity = 80/345 (23.19%), Postives = 141/345 (40.87%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEIT--IPFEKSIIALSLMENALTLSIKFH-GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFE---MFC-------------------NSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
++R KV LH A +D +LD+ +N D+ S + G T L +A ++ +L Q N K++ +PLH A G + L+ + NI+ GLT LHCA G E V + ++ GA + L L Q + + + ++ + ++ L + H G + + L + +NAR N + LH+A K + ++ + LL GA + +G +PL M C + + +I IL+ GA ++ ++ + T AS+L N I++ LL + + ++KD A
Sbjct: 166 DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKS---GFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQV-DATTKDMYTAL 506 HSP 3 Score: 60.4622 bits (145), Expect = 9.104e-9 Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K L +V LLL +I G P+H A G + IV LLQH D T G TP +A + ++TD I +
Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPT--VRGETPLHLAARANQTDIIRIL 455 HSP 4 Score: 59.3066 bits (142), Expect = 2.102e-8 Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHT 439
G+ P+H+A G N+V LL N++ GY P+H G+ IV LLL+HK + +T G TP IA+K ++ T
Sbjct: 700 GYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQT--VNGQTPLHIARKLGYISVLDSLKT 787 HSP 5 Score: 57.7658 bits (138), Expect = 6.802e-8 Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDC--IELFH 436
P+H+A + G N+V LLL + NIE + G P+H A +G+ ++V +LL+ I +TK GL P +A + D I L+H
Sbjct: 241 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKN--GLAPLHMAAQGEHVDAARILLYH 326 HSP 6 Score: 55.4546 bits (132), Expect = 2.812e-7 Identity = 26/90 (28.89%), Postives = 46/90 (51.11%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ G P+H+A G +NIVI LL + + + + G P+H A +I+++LL++ ++D R + TP IA + D + L
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQ--QTPLHIASRLGNVDIVMLL 488 HSP 7 Score: 54.6842 bits (130), Expect = 5.767e-7 Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
GH P+HIA K ++I LL E G+ P+H ++ G+ EI LL++HK ++ K GLTP + + + E+
Sbjct: 601 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKN--GLTPMHLCAQEDNVNVAEIL 686 HSP 8 Score: 54.299 bits (129), Expect = 7.816e-7 Identity = 34/104 (32.69%), Postives = 51/104 (49.04%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTEN---LIAAIKKN 469
+G P+H+A +I+ +LL ++ P+H A+ GN++IV LLLQH ++D TK + T IA K + D + EN L AA KK
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQ-DEVAAVLIENGAALDAATKKG 535
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0344224) HSP 1 Score: 87.8113 bits (216), Expect = 4.680e-17 Identity = 130/578 (22.49%), Postives = 233/578 (40.31%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQ-------SFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIX------XXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+HIA +K + LLL+ + N + G+ P+H A+ GN I LL+Q ++ K ++P +A KW +T+ + L ++K IE K GLT L AA S ++++ +L+R P+ K N L + +H A G + ++AA I H+ + L +AA GH ++ + N RA+ A K ++ E + + + T LH A ++V Y+L H + D+ + T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G + V ++ NGA + F + I + +K + +N +T L + H ++ L L+K S + A++G ++ LH+A + Q ++ +LL+ GA + + G +PL ++ ++ + EI+N+LI + A +N + N +T ++ +N + L N
Sbjct: 176 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK--HNISPLHVAAKWGKTNMVSLL--------LEKGGNIE------------AKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGL----------------------APLHMAAQG--EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDR---NADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNG--HTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPL---------HLSSQEGH--AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKN 689 HSP 2 Score: 61.2326 bits (147), Expect = 6.783e-9 Identity = 76/328 (23.17%), Postives = 134/328 (40.85%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEIT--IPFEKSIIALSLMENALTLSIKFH-GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFE---MFC-------------------NSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
++R KV LH A +D +LD+ +N D+ S + T L +A ++ +L Q N K++ +PLH A G + L+ + NI+ GLT LHCA G E V + ++ GA + L L Q + + + ++ + ++ L + H G + + L + +NAR N + LH+A K + ++ + LL GA + +G +PL M C + + +I IL+ GA ++ ++ + T AS+L N I++ LL +
Sbjct: 166 DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGF--TPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491 HSP 3 Score: 56.225 bits (134), Expect = 2.185e-7 Identity = 29/78 (37.18%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKK 403
G+ P+H+A G N+V LL N++ GY P+H G+ IV LLL+HK + +T G TP IA+K
Sbjct: 700 GYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVN--GQTPLHIARK 775 HSP 4 Score: 55.0694 bits (131), Expect = 4.009e-7 Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K L +V LLL +I G P+H A G + IV LLQH D T G TP +A + ++TD I +
Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR--GETPLHLAARANQTDIIRIL 455 HSP 5 Score: 52.373 bits (124), Expect = 3.186e-6 Identity = 83/368 (22.55%), Postives = 148/368 (40.22%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTEN---LIAAIKKN-DPIEFEK-----------LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR---PVENKEINDLMKEITEENDKLSNLTKECILYRSIIH-QHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFID--GIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEA 1192
+G P+H+A +I+ +LL ++ P+H A+ GN++IV LLLQH ++D TK + T IA K + D + EN L AA KK P+ L K D +Q G+T L A N ++ +L++ P + I +++ ++ + Y ++ + + AGF L +++++GH L I+ + N + + + EI E+ +++ K +T LH A +MV+++L + N+D + ++G T L H + +L +++ N Q QTPLH A
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQ-DEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQG-KNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQM-DIATTLLEYGALANAESKAGFTP------------------LHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDM---ATKAGYTPLHVASHFGQANMVRFLLQNGANVDAAT---SIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA 773 HSP 6 Score: 51.9878 bits (123), Expect = 3.835e-6 Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDC--IELFH 436
P+H+A + G N+V LLL + NIE + G P+H A +G+ ++V +LL+ I +TK GL P +A + D I L+H
Sbjct: 241 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKN--GLAPLHMAAQGEHVDAARILLYH 326 HSP 7 Score: 51.2174 bits (121), Expect = 5.843e-6 Identity = 71/301 (23.59%), Postives = 114/301 (37.87%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSENEIT-IPFEKSIIALSLMENALTLSIKFHGKLFLCFL-DKLNSTTSNARDGNNNSL--------LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKL 1813
++ K +T LH A + ++ +++ N LD+ + T L L H K ++L Q E + Q K TPLH A L+ + + G T LH A K ++ ++ GA N L + Q EI+ + E +N LT + LC D +N ++G N + LH+A Q + + LL +GA VD S G +PL + + I N+L+ + A+ N ++ N T A KL
Sbjct: 496 DATTKDMYTALHIAAKEGQDEVAAVLIE--NGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLT-------PMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHC-----------HIVNLLLEHKANANAQTVNGQTPLHIARKL 776
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303125) HSP 1 Score: 87.0409 bits (214), Expect = 8.141e-17 Identity = 130/578 (22.49%), Postives = 233/578 (40.31%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQ-------SFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIX------XXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+HIA +K + LLL+ + N + G+ P+H A+ GN I LL+Q ++ K ++P +A KW +T+ + L ++K IE K GLT L AA S ++++ +L+R P+ K N L + +H A G + ++AA I H+ + L +AA GH ++ + N RA+ A K ++ E + + + T LH A ++V Y+L H + D+ + T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G + V ++ NGA + F + I + +K + +N +T L + H ++ L L+K S + A++G ++ LH+A + Q ++ +LL+ GA + + G +PL ++ ++ + EI+N+LI + A +N + N +T ++ +N + L N
Sbjct: 176 LHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK--HNISPLHVAAKWGKTNMVSLL--------LEKGGNIE------------AKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGL----------------------APLHMAAQG--EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDR---NADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNG--HTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPL---------HLSSQEGH--AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKN 689 HSP 2 Score: 60.8474 bits (146), Expect = 8.542e-9 Identity = 76/328 (23.17%), Postives = 134/328 (40.85%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEIT--IPFEKSIIALSLMENALTLSIKFH-GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFE---MFC-------------------NSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
++R KV LH A +D +LD+ +N D+ S + T L +A ++ +L Q N K++ +PLH A G + L+ + NI+ GLT LHCA G E V + ++ GA + L L Q + + + ++ + ++ L + H G + + L + +NAR N + LH+A K + ++ + LL GA + +G +PL M C + + +I IL+ GA ++ ++ + T AS+L N I++ LL +
Sbjct: 166 DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGF--TPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491 HSP 3 Score: 55.8398 bits (133), Expect = 2.572e-7 Identity = 29/78 (37.18%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKK 403
G+ P+H+A G N+V LL N++ GY P+H G+ IV LLL+HK + +T G TP IA+K
Sbjct: 700 GYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVN--GQTPLHIARK 775 HSP 4 Score: 55.0694 bits (131), Expect = 4.410e-7 Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K L +V LLL +I G P+H A G + IV LLQH D T G TP +A + ++TD I +
Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR--GETPLHLAARANQTDIIRIL 455 HSP 5 Score: 51.9878 bits (123), Expect = 4.399e-6 Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDC--IELFH 436
P+H+A + G N+V LLL + NIE + G P+H A +G+ ++V +LL+ I +TK GL P +A + D I L+H
Sbjct: 241 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKN--GLAPLHMAAQGEHVDAARILLYH 326 HSP 6 Score: 51.2174 bits (121), Expect = 7.110e-6 Identity = 70/294 (23.81%), Postives = 111/294 (37.76%), Query Frame = 2
Query: 968 FTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSENEIT-IPFEKSIIALSLMENALTLSIKFHGKLFLCFL-DKLNSTTSNARDGNNNSL--------LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKL 1813
+T LH A + ++ +++ N LD+ + T L L H K ++L Q E + Q K TPLH A L+ + + G T LH A K ++ ++ GA N L + Q EI+ + E +N LT + LC D +N ++G N + LH+A Q + + LL +GA VD S G +PL + + I N+L+ + A+ N ++ N T A KL
Sbjct: 503 YTALHIAAKEGQDEVAAVLIE--NGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLT-------PMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHC-----------HIVNLLLEHKANANAQTVNGQTPLHIARKL 776 HSP 7 Score: 50.8322 bits (120), Expect = 7.801e-6 Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
GH P+HIA K ++I LL E G+ P+H ++ G+ EI LL++HK ++ K GLTP + + + E+
Sbjct: 601 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKN--GLTPMHLCAQEDNVNVAEIL 686
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44]) HSP 1 Score: 95.5153 bits (236), Expect = 2.329e-19 Identity = 129/558 (23.12%), Postives = 217/558 (38.89%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL-IAAIKKN--DPIEFEKLCKSYDFMSQKLHTG----------LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKEC----ILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG--TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADI 1762
++ G P+HIA LN+ LLLN ++ G P+H A+ GN+ +V+LLL +ID +TK LTP A + IE+ + I A KN PI D + Q L LT L AA ++ K +LD+ K T + + L C + ++ +H+A + + + + L +++ GH + +M+K + V+ + A E+ E + + +++ K + T LH A +MVK +L+H N SN+ T T L +A H + +L K TPLH A G D L+ R N + G +GLT LH AV +V +++ G + + S +N+ + A SL++ HG ++NA + LH+A + QP++ L+ A V++ N +G +PL + + + I +IL+ +GA +
Sbjct: 201 VLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDE--LTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGA---------KPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGL----------TPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKAN----SNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEV-------ASSLLQ---------HG------------ASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGH-----------VGIADILVKHGASV 694 HSP 2 Score: 72.4034 bits (176), Expect = 2.558e-12 Identity = 123/556 (22.12%), Postives = 221/556 (39.75%), Query Frame = 2
Query: 137 LEFVNKLID---------DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIEL---------FHTENLIA----AIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIA-----------TQSF-----LEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXE----IQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLC--FLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
LE V L+D + G P+ +A ++G N+V LL+N + +H A N + +LLQ+ D +K G TP IA + + +L F +N I A ++ + I L + K LT L AA + +II+ +LD+ P++ K N L + + D L + ++ + Y + I ++ A G H++A T++ L IA ++ H +D ++K + +++ + F ++ + + + S +KV T LH A ++ +++L + +D + ++ T L A + H + +K+L +++ +N+ TPLH A G L++ + + ++ G T LH A G +V ++ GAN P + L+ + A+ H L + L K S S AR+G + LH+A K Q E+ SLL GA + + G +PL
Sbjct: 124 LEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVR----LPALHIAARNDDTRTAAVLLQNDPNPDVLSK--TGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHL-DCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVE-TPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQ--TPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGAN-----------------------PNAAGKVGLTPLHVAV-----HHNNLDVVNLLLSKGGSPHSAARNG--YTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPL 639 HSP 3 Score: 60.8474 bits (146), Expect = 9.226e-9 Identity = 28/93 (30.11%), Postives = 48/93 (51.61%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
L + G P+H+ ++G + I +L+ ++ GY P+H A GNI++VK LLQ + ++C+T+ TP A + TD + L
Sbjct: 663 LGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTR--MSYTPLHQAAQQGHTDIVTLL 753 HSP 4 Score: 58.151 bits (139), Expect = 6.134e-8 Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+ P+H+AC G + +V LL + N+ C+ Y P+H A G+ +IV LLL+H + + T G + IAK+ I++
Sbjct: 701 GYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSN--GTSALAIAKRLGYISVIDVL 786 HSP 5 Score: 50.8322 bits (120), Expect = 9.786e-6 Identity = 84/333 (25.23%), Postives = 130/333 (39.04%), Query Frame = 2
Query: 1025 LDH-PNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLM-----ENALTLSIKFHG-----KLFLCFLDKLNSTTSNAR-----DGNNNSL-------LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN-PI---FKNFSSKDFQMAFDNHWFQYAECLLKLL 1942
LDH N ID+++ N+ L LA H K + L + + T K T LH A +G + L+N N++ G T L+ A + V + +++NGAN TIP E L++ EN + L I HG +L + N T A D N + L LH+A + + LL+ GA V+ NG +PL N + + +L+ GA I+ K+ + +T A++ + RI+ LL PI KN S A +H +C+ +LL
Sbjct: 29 LDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQ--------------------TIPTEDGFTPLAVALQQGHENVVALLIN-HGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGN-----------VIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHL----DCIRQLL 325
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6]) HSP 1 Score: 91.6633 bits (226), Expect = 2.722e-18 Identity = 131/622 (21.06%), Postives = 240/622 (38.59%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL-IAAIKKN--DPIEFEKLCKSYDFMSQKLHTG----------LTILQTAADSNADKIIKCILDRPVE--NKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHW---FQYAECLLKLLSDSF 1954
++ G P+HIA L++ LLLN N+ G P+H A+ GN+ +V+LLL +ID +TK LTP A + +E+ + + A KN PI D + Q L LT L AA +++K +LD+ + + +N I + + + ++ ++ +H+A + + + + L +AA GH + ++++ + V+ + A E+ + + + +++ K + T LH A ++VK +++H N DS T L +A H + ++L K TPLH A G D L+ R N + G GLT LH AV +V + +V+ G + + + +N++ +A SL++ +N+ + LH+A + QP++ L+ A V++ N NG +PL + + + I ++L+ GA + S T A N +++ LL N ++ + D + F + C L+ + SF
Sbjct: 234 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDE--LTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSM--------DLLLKHSASLEAVTESGL----------TPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANP--DSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLE-------VASSLLQYG---------------------ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGH-----------VGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQ-AHVNSKTRVSVLLLDASYTGLFSVSVCTLRTFAFSF 793
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6]) HSP 1 Score: 91.2781 bits (225), Expect = 5.154e-18 Identity = 124/585 (21.20%), Postives = 226/585 (38.63%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL-IAAIKKN--DPIEFEKLCKSYDFMSQKLHTG----------LTILQTAADSNADKIIKCILDRPVE--NKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKT------IYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
++ G P+HIA L++ LLLN N+ G P+H A+ GN+ +V+LLL +ID +TK LTP A + +E+ + + A KN PI D + Q L LT L AA +++K +LD+ + + +N I + + + ++ ++ +H+A + + + + L +AA GH + ++++ I++ + A E+ + + + +++ K + T LH A ++VK +++H N DS T L +A H + ++L K TPLH A G D L+ R N + G GLT LH AV +V + +V+ G + + + +N++ +A SL++ +N+ + LH+A + QP++ L+ A V++ N NG +PL + + + I ++L+ GA + S T A N +++ LL
Sbjct: 231 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDE--LTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSM--------DLLLKHSASLEAVTESGL----------TPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANP--DSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLE-------VASSLLQYG---------------------ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGH-----------VGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLL 754 HSP 2 Score: 61.6178 bits (148), Expect = 5.134e-9 Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+H+ ++G + I +L+ + ++ GY P+H A GNI++VK LLQ + ++ +T+ G TP A + TD + L
Sbjct: 701 GLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTR--LGYTPLHQAAQQGHTDIVTLL 786 HSP 3 Score: 57.3806 bits (137), Expect = 1.001e-7 Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+ P+H+AC G + +V LL + ++ + GY P+H A G+ +IV LLL+H + T G +P IAK+ I++
Sbjct: 734 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTN--GTSPLGIAKRLGYISVIDVL 819 HSP 4 Score: 50.8322 bits (120), Expect = 9.723e-6 Identity = 88/378 (23.28%), Postives = 152/378 (40.21%), Query Frame = 2
Query: 788 LEIAAEQGH-----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDD--NCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKS--IIALSLMENALTLSIKF--------------HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
L +A+++GH EL +GI +T K A+ A +++ E+ ++ + F+ L+ A +N ++VKY+L+H N L + E T LA+A H + +L Y T+ K LH IA+ DD L+ N D + G T LH A +V + ++N GAN + FT ++ + I + I+ L++ + K H ++ LD+ A+ N S +HMA + + + LL A++D + + +PL + RM + +L+ GA N ++ N T A K + R M LL ++
Sbjct: 79 LHLASKEGHVKMVLELLHNGIDLETTTKKG--NTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPT--EDGFTPLAVALQQGHENVVALLINY----GTKGKVRLPALH--IAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGAN---------VNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ--GAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVK-----------VLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHS 424
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Match: ank2b (ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165]) HSP 1 Score: 90.8929 bits (224), Expect = 5.302e-18 Identity = 132/583 (22.64%), Postives = 227/583 (38.94%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIE-FEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
G P+HIA G +N+ LLLN ++ G P+H A+ GN +V LLL +ID +T+ GLTP A + +EL T+N ++ + + D +E + L + + LT L AA ++ K +LD K N + + + + L C R + + + FI A +G I +F+ GH + +++ +R A+ A E+ + + ++R + + T LH A T++V+ +L H + D + N T L +A H VL + A + K TPLH A G + L+ R D G GLT LH A + V +++ GA+ + + + +N++ I +++ N T S + H ++ L + + G + LH+A + + + + L+ GA +D G +PL N++M N L+ GA +N K+ N T A++ N I+ LL
Sbjct: 214 GFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRD--GLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGY--TPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEI--ATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTS--LHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKM-----------VNFLLKSGASVNDKTKNGYTPLHQAAQQGNTHIINVLL 762 HSP 2 Score: 85.1149 bits (209), Expect = 3.813e-16 Identity = 122/556 (21.94%), Postives = 212/556 (38.13%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXX---XXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
+HIA K LLL + N + + G+ P+H A GN+ + LLL +D + G+TP +A K T+ + L K D GLT L AA S D ++ +L+R P+ + N L + + D + EC+ + + QH A + + L +AA GH ++ K + + K ++ E + + ++ + T +H A ++V +L N D +N TAL +A + ++ L + + + + QTPLH A G + L+ + D G T LH A +G +V ++ GA + + F S + + ++ S +N LT L + H K+ L LDK S + A++G + LH+A K Q E+ +LL GA+ ++ G P+ +S E+ +L+ GA +NV + + +T A++
Sbjct: 185 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARN--GITPLHVASKRGNTNMVHLLLDRGAQIDAKTRD--------------------GLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHV-----ECVKH---LLQHKAPVDDVT----------LDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQ--NGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNG--YTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHS-----------EMAALLLQRGAQVNVTTKSGLTSLHLAAQ 685 HSP 3 Score: 79.337 bits (194), Expect = 1.836e-14 Identity = 125/551 (22.69%), Postives = 196/551 (35.57%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF-------------------------------GLTPFDIAKKWSRTDCIELF-HTENLIAAIKKND--PIEFEKLCKSYDFMSQKLHTGL----------TILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKL-----SNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
G P+H A G V LLL + G P+H A ++E VK LLQHK +D T + G TP IA K +R +EL I AI ++ PI + + L G T L AA + ++++C+L N M + D+ S L K I+ ++ QH A +AA NG + L IAA +GH + ++ ++ K + + A E+ + + +R +S K T LH A ++ + +LD + + N T L +A + + L QY TN Q K P+H A G + L+ R ++ GLT LH A + V E +V GAN + +L + L ++ + + FL K ++ N + N + LH A + + LL GAK + + NGN+ L
Sbjct: 280 GLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLR--------NGAMVDARAREDQTPLHIASRLGKTEIV--QLLLQHMA----HPDAATANG------YTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFT-PLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGY--TPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGY---------------------------TPLIVACHYGNAKMVNFLLKSGASV-NDKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITVNGNTAL 779 HSP 4 Score: 63.5438 bits (153), Expect = 1.385e-9 Identity = 95/422 (22.51%), Postives = 169/422 (40.05%), Query Frame = 2
Query: 788 LEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN-----------------------NETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
L +AA++GH + ++ + S + A+ A ++ + +++R ++ + FT L+ A +N D+V+Y+L++ N + + N+T G AL +A K +L Q + + Q+K TPLH A G + L+NR +D G+T LH A +G N+ +++ GA C + + + + E+ L+ +N L+ L + G C + L + D + L LH+A C + K LLD A + NG +PL + C N +++ +LI YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 57 LHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVEC-VKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------VKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLR 466 HSP 5 Score: 62.3882 bits (150), Expect = 2.967e-9 Identity = 90/371 (24.26%), Postives = 145/371 (39.08%), Query Frame = 2
Query: 152 KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF--HTENLIAAIKKN-DPIEFEKLCKSYDFMSQKLHT----------GLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAA---GFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASG 1204
+ I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L H + AA P+ D + L G T L A+ + ++ K +L R P ++ N L + D N +L HA G+ AA N +IAT + L+ AE T V I A + E+ + +R K T LH A ++ + + ++ N+D + LG T L +A + K + L + + N + K TPLH+A G
Sbjct: 406 QAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAR--EDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYD---NQKVALLLLDKGASPHATAKNGYTPLHIAAKKN-QMEIAT-TLLQYGAE-------------TNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTK---LGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQQG 753
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6]) HSP 1 Score: 90.8929 bits (224), Expect = 5.546e-18 Identity = 124/585 (21.20%), Postives = 226/585 (38.63%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL-IAAIKKN--DPIEFEKLCKSYDFMSQKLHTG----------LTILQTAADSNADKIIKCILDRPVE--NKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKT------IYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
++ G P+HIA L++ LLLN N+ G P+H A+ GN+ +V+LLL +ID +TK LTP A + +E+ + + A KN PI D + Q L LT L AA +++K +LD+ + + +N I + + + ++ ++ +H+A + + + + L +AA GH + ++++ I++ + A E+ + + + +++ K + T LH A ++VK +++H N DS T L +A H + ++L K TPLH A G D L+ R N + G GLT LH AV +V + +V+ G + + + +N++ +A SL++ +N+ + LH+A + QP++ L+ A V++ N NG +PL + + + I ++L+ GA + S T A N +++ LL
Sbjct: 212 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDE--LTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSM--------DLLLKHSASLEAVTESGL----------TPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANP--DSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLE-------VASSLLQYG---------------------ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGH-----------VGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLL 735 HSP 2 Score: 61.2326 bits (147), Expect = 6.226e-9 Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+H+ ++G + I +L+ + ++ GY P+H A GNI++VK LLQ + ++ +T+ G TP A + TD + L
Sbjct: 682 GLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTR--LGYTPLHQAAQQGHTDIVTLL 767 HSP 3 Score: 56.9954 bits (136), Expect = 1.214e-7 Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+ P+H+AC G + +V LL + ++ + GY P+H A G+ +IV LLL+H + T G +P IAK+ I++
Sbjct: 715 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTN--GTSPLGIAKRLGYISVIDVL 800
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806]) HSP 1 Score: 87.8113 bits (216), Expect = 7.629e-17 Identity = 139/589 (23.60%), Postives = 235/589 (39.90%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V +++ GA+ + + S +N++ I +++ N LT + + H + L+K +N G N L LH+A + + + + L +GA +D G SPL + C+ + I++ N L+ +GA++N K+ N T A++ + I+ LL N
Sbjct: 231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIA--TTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK----QANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLI-VACHYGN----------IKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 783 HSP 2 Score: 60.077 bits (144), Expect = 2.467e-8 Identity = 85/341 (24.93%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L S LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 186 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTS-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 485 HSP 3 Score: 56.6102 bits (135), Expect = 3.000e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 516
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806]) HSP 1 Score: 87.8113 bits (216), Expect = 7.964e-17 Identity = 139/589 (23.60%), Postives = 235/589 (39.90%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V +++ GA+ + + S +N++ I +++ N LT + + H + L+K +N G N L LH+A + + + + L +GA +D G SPL + C+ + I++ N L+ +GA++N K+ N T A++ + I+ LL N
Sbjct: 210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIA--TTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK----QANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLI-VACHYGN----------IKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 762 HSP 2 Score: 60.077 bits (144), Expect = 2.641e-8 Identity = 85/341 (24.93%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L S LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 165 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTS-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 464 HSP 3 Score: 56.6102 bits (135), Expect = 3.131e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 406 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 495
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806]) HSP 1 Score: 87.4261 bits (215), Expect = 9.825e-17 Identity = 139/589 (23.60%), Postives = 235/589 (39.90%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V +++ GA+ + + S +N++ I +++ N LT + + H + L+K +N G N L LH+A + + + + L +GA +D G SPL + C+ + I++ N L+ +GA++N K+ N T A++ + I+ LL N
Sbjct: 210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIA--TTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK----QANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLI-VACHYGN----------IKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 762 HSP 2 Score: 59.6918 bits (143), Expect = 2.799e-8 Identity = 85/341 (24.93%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L S LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 165 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTS-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 464 HSP 3 Score: 56.225 bits (134), Expect = 3.235e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 406 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 495
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806]) HSP 1 Score: 87.4261 bits (215), Expect = 1.238e-16 Identity = 140/589 (23.77%), Postives = 235/589 (39.90%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +AA GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V +++ GA+ + + S +N++ I +++ N LT + + H + L+K +N G N L LH+A + + + + L +GA +D G SPL + C+ + I++ N L+ +GA++N K+ N T A++ + I+ LL N
Sbjct: 227 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPI------HVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIA--TTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK----QANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLI-VACHYGN----------IKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 779 HSP 2 Score: 59.6918 bits (143), Expect = 3.354e-8 Identity = 85/341 (24.93%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L S LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 182 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTS-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 481 HSP 3 Score: 56.225 bits (134), Expect = 3.413e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 423 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 512
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806]) HSP 1 Score: 87.4261 bits (215), Expect = 1.248e-16 Identity = 140/589 (23.77%), Postives = 235/589 (39.90%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +AA GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V +++ GA+ + + S +N++ I +++ N LT + + H + L+K +N G N L LH+A + + + + L +GA +D G SPL + C+ + I++ N L+ +GA++N K+ N T A++ + I+ LL N
Sbjct: 227 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPI------HVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIA--TTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK----QANIHVGTKNGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLI-VACHYGN----------IKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 779 HSP 2 Score: 59.6918 bits (143), Expect = 3.354e-8 Identity = 85/341 (24.93%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L S LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 182 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTS-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 481 HSP 3 Score: 56.225 bits (134), Expect = 3.442e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 423 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 512
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 97.4413 bits (241), Expect = 6.950e-20 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPL-HVGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 59.3066 bits (142), Expect = 2.717e-8 Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK G T A + T I +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKN--GYTALHQAAQQGHTHIINVL 765 HSP 3 Score: 54.6842 bits (130), Expect = 6.883e-7 Identity = 27/101 (26.73%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H+A +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDA--AGKSGLTPLHVAAHY 591 HSP 4 Score: 53.1434 bits (126), Expect = 2.366e-6 Identity = 76/326 (23.31%), Postives = 129/326 (39.57%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++V+++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C + E + + ++S LS +N L+ L + G C L D N+ L LH+A C ++ K LLD A + NG +PL + C N I + +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 123 GFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP-VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPL-HIACKKNR----------IRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 436
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 97.0561 bits (240), Expect = 7.672e-20 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLH-VGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 59.3066 bits (142), Expect = 3.003e-8 Identity = 23/73 (31.51%), Postives = 44/73 (60.27%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF 373
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGY 747 HSP 3 Score: 54.6842 bits (130), Expect = 8.203e-7 Identity = 27/101 (26.73%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H+A +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDA--AGKSGLTPLHVAAHY 591 HSP 4 Score: 53.1434 bits (126), Expect = 2.302e-6 Identity = 76/326 (23.31%), Postives = 129/326 (39.57%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++V+++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C + E + + ++S LS +N L+ L + G C L D N+ L LH+A C ++ K LLD A + NG +PL + C N I + +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 123 GFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP-VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLH-IACKKN----------RIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 436
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 97.0561 bits (240), Expect = 8.791e-20 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 229 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPL-HVGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 780 HSP 2 Score: 59.3066 bits (142), Expect = 2.772e-8 Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK G T A + T I +
Sbjct: 686 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKN--GYTALHQAAQQGHTHIINVL 776 HSP 3 Score: 55.0694 bits (131), Expect = 6.504e-7 Identity = 27/101 (26.73%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H+A +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 504 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDA--AGKSGLTPLHVAAHY 602 HSP 4 Score: 52.7582 bits (125), Expect = 3.299e-6 Identity = 76/326 (23.31%), Postives = 129/326 (39.57%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++V+++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C + E + + ++S LS +N L+ L + G C L D N+ L LH+A C ++ K LLD A + NG +PL + C N I + +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 134 GFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP-VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPL-HIACKKN----------RIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 447
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 97.0561 bits (240), Expect = 9.736e-20 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLH-VGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 59.3066 bits (142), Expect = 2.954e-8 Identity = 23/73 (31.51%), Postives = 44/73 (60.27%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF 373
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGY 747 HSP 3 Score: 54.6842 bits (130), Expect = 7.042e-7 Identity = 27/101 (26.73%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H+A +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDA--AGKSGLTPLHVAAHY 591 HSP 4 Score: 52.7582 bits (125), Expect = 3.280e-6 Identity = 76/326 (23.31%), Postives = 129/326 (39.57%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++V+++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C + E + + ++S LS +N L+ L + G C L D N+ L LH+A C ++ K LLD A + NG +PL + C N I + +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 123 GFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP-VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLH-IACKKN----------RIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 436
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 96.6709 bits (239), Expect = 1.069e-19 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLH-VGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 59.3066 bits (142), Expect = 3.018e-8 Identity = 23/73 (31.51%), Postives = 44/73 (60.27%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF 373
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGY 747 HSP 3 Score: 54.6842 bits (130), Expect = 7.438e-7 Identity = 27/101 (26.73%), Postives = 47/101 (46.53%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
GK + +++ L+ G+ P+H+A +G ++ LL+ ++ G+ P+H A G +E+ LLLQ D G GLTP +A +
Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDA--AGKSGLTPLHVAAHY 591 HSP 4 Score: 52.7582 bits (125), Expect = 2.901e-6 Identity = 76/326 (23.31%), Postives = 129/326 (39.57%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++V+++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C + E + + ++S LS +N L+ L + G C L D N+ L LH+A C ++ K LLD A + NG +PL + C N I + +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 123 GFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP-VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLH-IACKKN----------RIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 436
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 7.646e-19 Identity = 134/593 (22.60%), Postives = 235/593 (39.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLT-------KECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + LT L AA K+ K +LD K+ + L+ T K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L ++ + T L LA H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ I S++ NA+T S+ G + + L L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD------------KKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ--GASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDI--ATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSL--LLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLH-VGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNN 769 HSP 2 Score: 58.9214 bits (141), Expect = 2.393e-7 Identity = 23/73 (31.51%), Postives = 44/73 (60.27%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF 373
L + G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK +
Sbjct: 675 LSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGY 747
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3) HSP 1 Score: 93.5893 bits (231), Expect = 7.970e-18 Identity = 136/594 (22.90%), Postives = 238/594 (40.07%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDF-MSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDH---PNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLME-----NALT----LSIKFHGKL-FLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
+ G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + + LT L AA K+ K +LD K+ N K + K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L PN + + T L L+ H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ IA SL+E NA+T S+ + + + L S +N N + L LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 241 ESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD-----KKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPN-----AATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMD-------IATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPL-HVGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNN 794 HSP 2 Score: 61.2326 bits (147), Expect = 5.643e-8 Identity = 33/109 (30.28%), Postives = 55/109 (50.46%), Query Frame = 2
Query: 119 LRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTEN 445
L R + NK G P+H+A ++ +N+ +L+N+ +++ + GY P+H GNI+IV LLQH +++ +TK G TP A + T I + N
Sbjct: 692 LSRNANVNLSNK----SGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKN--GYTPLHQAAQQGHTHIINVLLQNN 794
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Match: sp|Q12955|ANK3_HUMAN (Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=3) HSP 1 Score: 88.5817 bits (218), Expect = 2.352e-16 Identity = 135/600 (22.50%), Postives = 240/600 (40.00%), Query Frame = 2
Query: 149 NKLIDDKGHF-PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDF-MSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDH---PNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLME-----NALT----LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
N ++ K F P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL +ID +T+ GLTP + +E+ T+N ++ + + D + +L ++ + + LT L AA K+ K +LD K+ N K + K I ++ +H A + + + + + +AA GH + +M + VR A+ A E+ + + E++ K + T LH + D+V+ +L PN + + T L L+ H L + + + K TPLH A G + L+ +S + D G GLT LH A + V +++ GA+ + + + +N++ IA +L+E NA+T S+ G + + L + N + + + LH+A + + + + L++ GA VD G +PL + C+ + I+I N L+ + A +N K+ N T A++ + I+ LL NN
Sbjct: 227 NADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRD--GLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD-----KKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPN-----AATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMD-------IATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPL-HVGCHYGN----------IKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNN 786 HSP 2 Score: 56.225 bits (134), Expect = 1.776e-6 Identity = 76/325 (23.38%), Postives = 130/325 (40.00%), Query Frame = 2
Query: 968 FTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLGT----ALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTC---QSSENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
FT L+ A +N ++VK++LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR+ +D +T LH A +G N+ + +++ GA C E + + +++ LS +N L+ L + G C + L D N+ L LH+A C ++ K LLD A + NG +PL + C N I++ +L+ +GA I + + +T A+ + + I+ L+
Sbjct: 141 FTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNC-VQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPL-HIACKKNR----------IKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 453
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2) HSP 1 Score: 87.0409 bits (214), Expect = 7.599e-16 Identity = 134/587 (22.83%), Postives = 229/587 (39.01%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALT---------LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K Y + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +AA GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V ++ GA+ + + + +N++ I +++ N +T S + H + LDK + + + G + LH+A + + + L GA D G +PL + C+ + +++ N L+ GA++N K+ N T A++ + I+ LL +
Sbjct: 231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPI------HVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIA--STLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLI-VACHYGN----------VKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 783 HSP 2 Score: 60.077 bits (144), Expect = 1.427e-7 Identity = 111/546 (20.33%), Postives = 205/546 (37.55%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTI---YNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
+H+A ++G + +V LL +++ G +H A+L G E+VK+L++ I+ +++ G TP +A + EN I +K L ++ S G T L A ++ + +L+ + K + ++D TK L H K +N +G + L IAA G+ ++ + + + + A + + + + +R +++ + T LH A +V+ +L+ + + N + L +A H +C+K L QY+ + T LH A G L+++ N + G T LH A K V E +V GA+ +Q +S I + F G L + L N + + + + LHMA + Q E+ + LL +GA VD +PL + SR+ + EI +L+ + A + +TN T +++
Sbjct: 68 LHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQN--GFTPLYMAAQ------------ENHIDVVKY--------LLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDD-----TKSAALLLQNDHNADVQSKMMVNRTTESG------FTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGL--SPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGAS---------IQAITESGLTPIHVA-------------------AFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPL-----HIASRLGK------TEIVQLLLQHMAHPDAATTNGYTPLHISAR 539 HSP 3 Score: 57.7658 bits (138), Expect = 6.356e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 516
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Match: sp|Q01484|ANK2_HUMAN (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4) HSP 1 Score: 84.7297 bits (208), Expect = 3.458e-15 Identity = 133/585 (22.74%), Postives = 229/585 (39.15%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP-------FEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
+ G P+HIA G +N+ LLLN ++ G P+H A+ GN +VKLLL +ID +T+ GLTP A + +EL T+N ++ + + D +E K L + + LT L AA ++ K +LD K N + + + + L C R + + + I A +G I +F+ GH + +++ +R A+ A E+ + L ++R + T LH A T++V+ +L H + D + N T L ++ VL + A + K TPLH A G D L+ R D G GLT LH A + V ++ GA+ + + + +N++ I E +I+ + S + H + LDK + + + G + LH+A + + + L GA D G +PL + C+ + +++ N L+ GA++N K+ N T A++ + I+ LL +
Sbjct: 231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRD--GLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD-----KRANPNARAL----NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM------GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGY--TPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLI-VACHYGN----------VKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQH 783 HSP 2 Score: 61.2326 bits (147), Expect = 6.300e-8 Identity = 84/341 (24.63%), Postives = 130/341 (38.12%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSN---NETLGTALALAHSLHHRKCIKV---LQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKD-FQMAFDNHWFQYAECLLK 1936
+++ KV LH A +DT +L + +N D+ S N T + H H + V L A + + TPLH A G + L++R ID GLT LHCA G + V E ++ GA + + L+ L Q E K ++ + +T LD L + LH+A C + K LLD A + NG +PL + C N I++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 186 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECV----KHLLQHKAPVDDVT-------------LDYLTA-------------LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPL-HIACKKNR----------IKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLR 485 HSP 3 Score: 57.3806 bits (137), Expect = 7.790e-7 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
I + G PIH+A G LNIV+LLL + + +I G +H A G +E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE--EQTPLHIASRLGKTEIVQLL 516
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Match: A0A3L8DCQ5 (Uncharacterized protein OS=Ooceraea biroi OX=2015173 GN=DMN91_008646 PE=4 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 5.747e-19 Identity = 148/675 (21.93%), Postives = 275/675 (40.74%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIEL-------------FHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRP--VENKEIN--DLMKEITEENDKLSNL----------TKECILYRSIIHQHAAGFKKFI-----NAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKV----NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYE----------------LAT------------------NTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSI--KFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKD---FQMAFDNHWFQYAECLLKLLSDSFLESNITNE 1978
P+H+A G +I+ LL+ + +DI G PIH A NGNI+++ LL+++K ++ RT +TP +A +R D I + L +A+ + E L K + + T+L A ++ +I+K ++ + K N L+ I N +++ + L+ +++ AG+K + NAA +N + + L +AA +GH+ ++ ++K +I + + + +E +EI E + + + V T+LH+A + +++V+ +L+ NI++ N LA+AH H + +K L QY+ +A+ N +N P+H A G D + +++ +NI+ G + T LH A +KG V E ++ GA+ Q S + F + L++N ++ K K + DK T + + ++ H V ++ E + GA V+ N+NG +PL ++R I N+L+ A+ N T A+K + +++ ALL+N ++ + D D + + +LLSDSF NI N+
Sbjct: 394 PLHVAALNGNYSIIDLLIKNKAEVNAQDIHGNTPIHEAATNGNIDVIDLLIKNKIDVNVRTHN--DVTPLHLAALKNRIDAIVFLIKSGAAVNARANYGVTPLHSAVLEGHKNVVELLIKHTAEVDARAIADGTLLHMAVEAGHTEIVKILVANGANINTKHTNRTPLLSAIKNNNKEIAEILIANGADVDEEDSGPLFLAVL----AGYKDIVEMLLKNAARVN-IRGLENATPLHLAATRGHKEIVNALIKSGADIDAITITDV-TSLYVAVQEGHKEIVEILIANGANVNVKSTDGTTLLHTAAGNGHSNVVQVLLNSKVNINIKDNKNRTSLELAVAHG--HLEVVKTLFQYQKIDMNTKGNDNWTILHIASQESNLDMVKYLVDNGSDINAKNASGSKPMHIAAREGHKDTVEFFLSKGLNINDPGAVNQTLLHYAAMKGHLAVVEYLIAQGADVNS-------QDKNGVSPMHLAATFGYKNVIEVLLKNGAIYNVVDKLCRKPLIMSSDKTVITLLASTENLFKAIQHNNVLEVETE-----IKMGAFVNTKNANGGTPLHYAAWKGSNR-----------IVNLLLQNRANSNAVGKKGFTPLHYAAKFSHLKVVKALLANGAVYNAVTDDDKTPLNFTVDKDIIR----IFQLLSDSF--KNINND 1029 HSP 2 Score: 100.908 bits (250), Expect = 1.248e-17 Identity = 147/584 (25.17%), Postives = 233/584 (39.90%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTD--CIELFHTENLIAAIK--KNDPIEF----------EKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFI-NAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANY-MKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKL---------NSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLS 1846
G+ P+H A E G L +V LL N + + PIH A LNG++EIV +L+ D K G TP A + SR I L H N+ A K N + + + L K S K G+T L A S +I+ +L V DK +N L+ ++ H + I N A +N + I ++ L +AA G+ ID ++K NK+ E+ + I N T +H A + + D++ ++ + ++++ ++N+ T L LA + I L + A N + Y TPLH A+ G + LI + +D T LH AV G + + +V NGAN K L +++ E IA L+ N + + G LFL L N+ N R N + LH+A E+ +L+ SGA +D + + L+ + EI ILI GA++NVKST+ T+ +A+ + ++ LL+
Sbjct: 192 GYTPLHEAAEHGHLELVNFLLEYGANSNARNDRDWTPIHGAALNGHLEIVNVLISEGA--DVNAKVLDGCTPLHYAVETSREKIASILLKHGANVNAVDKTYNNTALHYAAKDGHEGIVKTLLKHKANASVKTVGGITPLHLAVHSGHLEIVTILLKHGVN-----------IHAKDKNNN----TPLHYAVNSAHMEVVEHLIKNGAEVNAKNCI-DKTPLHVAALNGNYSIIDLLIK----NKA---------------EVNAQ----------DIHGN-TPIHEAATNGNIDVIDLLIKNKIDVNVRTHNDV--TPLHLAALKNRIDAIVFLIKSGAAVNARANYGVTPLHSAVLEGHKNVVELLIKHTAEVDARAIADGTLLHMAVEAGHTEIVKILVANGANINTKHTNRTPLLSAIKNNNKE---------IAEILIANGADVDEEDSGPLFLAVLAGYKDIVEMLLKNAARVNIRGLENATPLHLAATRGHKEIVNALIKSGADIDAITITDVTSLYVAVQEGHK-----------EIVEILIANGANVNVKSTDGTTLLHTAAGNGHSNVVQVLLN 705 HSP 3 Score: 88.5817 bits (218), Expect = 5.948e-14 Identity = 136/617 (22.04%), Postives = 253/617 (41.00%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKN----DPIEFEKLCKSYDFM-----------SQKLHTGLTILQTAADSNADKIIKCILDRPVENK-EINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVI---NGPHKIATQSF----LEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESR------IKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANY-MKFVE-LNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKF---HGKL-FLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL-----------FEMFCNSNSRMTEE-KVNRM----------IEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIF 1861
+H A + G IV LL N + + G P+H A +G++EIV +LL+H I + K TP A + + +E +N KN P+ L +Y + +Q +H I + A + N D ++D ++NK ++N + ND + H A K I+A V +G A ++ L A +GH+ ++ ++K T + RAI ++ TE + + + N T L SAI +N+ ++ + ++ N D+D E L LA ++ +++L + N + + TPLH A G + LI +ID I +T L+ AV +G + + E ++ NGAN +K + L + + + S + +++ +N S++ HG L + L + N + +N ++LH+A + ++ K L+D+G+ ++ N++G+ P+ E F + + + VN+ + + LI GAD+N + N ++ A+ + ++ LL N I+
Sbjct: 296 LHYAAKDGHEGIVKTLLKHKANASVKTVGGITPLHLAVHSGHLEIVTILLKHGVNIHAKDKN--NNTPLHYAVNSAHMEVVEHL-IKNGAEVNAKNCIDKTPLHVAALNGNYSIIDLLIKNKAEVNAQDIHGNTPIHEAATNGNID-----VIDLLIKNKIDVN-----VRTHND--------------VTPLHLAALKNRIDAIVFLIKSGAAVNARANYGVTPLHSAVLEGHKNVVELLIKHT---AEVDARAIADGTLLHMA-VEAGHTEIVKILVANGANINTKHTNRTPLLSAIKNNNKEIAEILI--ANGADVD---EEDSGPLFLAVLAGYKDIVEMLLKNAARVNIRGLENATPLHLAATRGHKEIVNALIKSGADIDAITITDVTSLYVAVQEGHKEIVEILIANGANVNVKSTDGTTLLHTAAGNGHSNVVQVLLNSKVNINIKDNKNRTSLELAVAHGHLEVVKTLFQYQKIDMNTKGNDNWTILHIASQESNLDMVKYLVDNGSDINAKNASGSKPMHIAAREGHKDTVEFFLSKGLNINDPGAVNQTLLHYAAMKGHLAVVEYLIAQGADVNSQDKNGVSPMHLAATFGYKNVIEVLLKNGAIY 876
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Match: W5MI54 (Ankyrin 3a OS=Lepisosteus oculatus OX=7918 PE=4 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 7.623e-19 Identity = 140/598 (23.41%), Postives = 242/598 (40.47%), Query Frame = 2
Query: 143 FVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLME-NALTLSIKFHG--KLFLCFLDK-------LNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
VN+ + G P+HIA G +N+ LLLN ++ P+H A+ GN +VKLLL+ RID +TK GLTP + +E+ T+N ++ + + D + +L +D + LT L AA K+ K +LD K+ N K + K I ++ +H A + + + + + +AA GHE + + + VR A+ A E+ + + + E++ K + T LH A D+V+ +L H D++ + T L LA H VL + + +T K TPLH A G + L+ + D G GLT LH A + V +++ GA+ + + + +N++ IA +L+E A T +I G + L + L + +N GN + L LH+A + + + + L++ GA VD G +PL + C+ + +++ N L+ + A +N K+ N T A++ + I+ LL +
Sbjct: 224 MVNRTTE-SGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNGNMVKLLLERGARIDAKTKD--GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD-----KKANPNAKALNGFTPLHIASKKNRIKVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHENIVTQLTHHGASPNTTNVRGETALHMAARAGQAEVVKYLVQNGAQVEAKAKDDQTPLHIASRLGKADIVQQLLQH--GASPDASTTSGYTPLHLAAREGHEDVASVLLEQGASLSTTTKKGFTPLHVAAKYGKIEVANLLLQKKATPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMD-------IATTLLEYGADTNAITRQGISPVHLAAQEGNVDVVSLLLARNANVNLGNKSGLTPLHLAAQEDRVNVAEVLVNQGATVDPQTKMGYTPLH-VACHYGN----------VKMVNFLLQHQAKVNAKTKNGYTPLHQAAQQGHTHIINILLQHG 783
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Match: A0A1B6CUN0 (Uncharacterized protein (Fragment) OS=Clastoptera arizonana OX=38151 GN=g.13255 PE=4 SV=1) HSP 1 Score: 103.99 bits (258), Expect = 1.296e-18 Identity = 152/603 (25.21%), Postives = 255/603 (42.29%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL--IAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCI-LDRPVEN---KEINDLMKEITEENDKLSNLTKECILYRSIIHQHAA--GFKKFINAAVINGPHK----IATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR------AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDH-------------------------------PNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSEN-----EITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSAS 1807
D+ G P+HIA + G + V +LLN N N +D G+ P+HYA +I++ K++L+ + +D + + G T IA + + + F EN + A ++N I ++ L ++ AD N+ I C L VEN K N L+K +N+ I+Y + +AA G +K + A +IN + +A + L A + GH + +++ + I + + A KEI E + ++ + K NFT L+ A+ D+++ ++ + N D+ + N T L LA H+ I L + + NT+N + TPLH A+ G D +LI ++ G T LH AV G + + + +V NGA Y+ NF C N E+ I S+ A S +ALTL++ + + L K N T N + +LLH AV+ E+ +L+ GA V+ + ++G +PL+ +R E EI +ILI GA +NV + + ++A
Sbjct: 950 DNSGKTPLHIAAKNGHKDAVEILLNNNANTNTKDKRGFSPLHYAITYNHIDVAKIMLEREANVDI-NESFGGFTSLHIAAENGYLELVN-FLLENGANVNAKQENVGIPLHTAA-----LNGHLEVVNALILKGADVNSRVIDGCTPLHYAVENGYEKIANILIKH-------GANVNVVDIVYNNTPLHYAAQDGHEKIVEALLINKANASVATVAGVTPLHCAVKSGHLKIVASLLEHGV---DIHAKDKNNASPLHFAVKCGHKEIVELLIKKGAKINDKDKHNFTPLYCAVVGGHKDIIELLIKNKADVKVKDIKGGTLLHAAAMNGKTDVIALLIKNKADVFARNNDGLTPLHLAAFGGHKDAIDFLIKSKAKVNTRNNHGVTPLHGAVIGGQKDIVTFLIQNKAKVNVEGHADSTPLHLAVEGGHKEIVKILVANGA-YVNVKINNFTPLICAVRHNHKEIVEVLIENGASVNAESW--DALTLAVLSSNRDIVEILLK-NITNINMKGPKGLTLLHSAVERGNKEIVNTLIAMGANVNAMTTDGTTPLYVA-----AREGHE------EIADILIANGAMVNVVKADETPLHRAAG 1520 HSP 2 Score: 73.1738 bits (178), Expect = 3.796e-9 Identity = 124/593 (20.91%), Postives = 230/593 (38.79%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKI-----ATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSENEITIPFE---KSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLF----------------------EMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIF 1861
P+H A + G IV LL+ + I +D + P++ A + G+ +I++LL+++K D + K G T A +TD +IA + KN F ++ D GLT L AA I + I K T N ++ L H G +K I +I K+ A + L +A E GH+ + ++ Y + ++N NFT L A+ N ++V+ ++++ +++ +S AL LA +R +++L + N + T LH A+ G + LI N++ + G T L+ A +G E + + ++ NGA N +K E + N + + K + + L L+ + + L + NA+ + ++LH+A + E+ K L+D G ++ N++G+ P+ E+ ++ + + + +E+ +I GAD+N K N +T A+ + I+ LL + I+
Sbjct: 1189 PLHFAVKCGHKEIVELLIKKGAKINDKDKHNFTPLYCAVVGGHKDIIELLIKNKA--DVKVKDIKGGTLLHAAAMNGKTD---------VIALLIKNKADVF---ARNND--------GLTPLHLAAFGGHKDAIDFL---------IKSKAKVNTRNNHGVTPL-----------HGAVIGGQKDIVTFLIQNKAKVNVEGHADSTPLHLAVEGGHKEIVKILVANGAY-----------------------VNVKIN--------NFTPLICAVRHNHKEIVEVLIENGASVNAES-----WDALTLAVLSSNRDIVEILLKNITNINMKGPKGLTLLHSAVERGNKEIVNTLIAMGANVNAMTTDGTTPLYVAAREGHEEIADILIANGAMVNVVKADETPLHRAAGHGHINVVEVLLRNGAKVHVKDYMNRTPLELAASYGHLHVVKMLLQYKKVDLNAKGNDGWTILHIASQDSNLEMVKFLVDEGVNINAKNTSGSKPIHIAAREGLKDTVQFFLSKGMSVNELGADNQTLLHYAAMKNGLEVIKYVIAQGADVNAKDNNGLTPMHIAALYGYKDIIKVLLKHGAIY 1703 HSP 3 Score: 65.4698 bits (158), Expect = 7.689e-7 Identity = 120/574 (20.91%), Postives = 218/574 (37.98%), Query Frame = 2
Query: 191 ACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTE----------------NLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITE-----------------ENDKLSNLTKECILYRSIIHQHAA---GFKKFINAAVINGP----HKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKV----NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTN 1780
A EKG L + L + +I +I +H+A ++E+++ ++ HK ++ K G +P IA R + +E +E ++ A D +E + K G + L A N + K +L+R N +IN+ T EN N +E + I H A G + +NA ++ G I + L A E G+E + ++K + + ++ E+I E + + ++ V T LH A+ +V +L+H +D+ + ++ + L A H++ +++L + N ++K++ TPL+ A+ G D LI ++ G T LH A + G +V ++ N A+ + N+ P + A ++A+ IK K+ N R+ + + LH AV Q ++ L+ + AKV+V ++PL + EI IL+ GA +NVK N
Sbjct: 860 ALEKGNLEDLKSYLRKGADINARNINSSTTLHFAAHGPSLEVIQFIIDHKLSVNV--KNVNGQSPLHIAAVHGRKNIVEFLISETGVNIDDQDNSGKTPLHIAAKNGHKDAVEILLNNNANTNTKDK--RGFSPLHYAITYNHIDVAKIMLEREA-NVDINESFGGFTSLHIAAENGYLELVNFLLENGANVNAKQENVG----IPLHTAALNGHLEVVNALILKGADVNSRVIDGCTPLHYAVENGYEKIANILIKHGANVNVVDIVYNNTPLHYAAQDGHEKIVEALLINKANASVATVAGVTPLHCAVKSGHLKIVASLLEH--GVDIHAKDKNNASPLHFAVKCGHKEIVELLIKKGAKINDKDKHNFTPLYCAVVGGHKDIIELLIKNKADVKVKDIKGGTLLHAAAMNGKTDVIALLIKNKADVF-------------ARNNDGLTPLH--LAAFGGHKDAIDFLIKSKAKV-------------NTRNNHGVTPLHGAVIGGQKDIVTFLIQNKAKVNVEGHADSTPLHLAVEGGHK-----------EIVKILVANGAYVNVKINN 1383
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Match: Q16FR1 (AAEL014668-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014668 PE=4 SV=1) HSP 1 Score: 103.219 bits (256), Expect = 1.345e-18 Identity = 138/574 (24.04%), Postives = 238/574 (41.46%), Query Frame = 2
Query: 125 RTLRLEFVNKLIDDK---------GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEK-LCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFI-NAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDK-LNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
R LE V LID++ G P+H A + G L++V LL++ N++ G P+HYA+ NGN+E+VKLL+ ++ +D T + G TP A + + D + L A +N +E K L ++ + + G T L ++ + K++K + +ENK D T +N+ + L+ + + H K I N A ++ + + SF I ++ G + + + I R N+ ++ +T LH A + ++VK+++D N + D+ N T+ +A + +K+L +T N TPLH A +G + LI+ N+D G T H G V + +++N AN +++NE P + S H ++ +D N T N R ++ H+A K + E+ K L+D+GA VD N+ G +PL SR N +E+ +LI GA+++ K+ T F S+
Sbjct: 338 RNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVD--TAQYEGWTPLHYASRNANVDTTQNEGCTPLHYA-SRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLL----IENKANVD-----TTQNEGWT------PLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSF-HIVSQNGRLVLVKLL-----------------------------IDNRANV-DTTDNEGWTPLHYASQNGHLEVVKFLID--NGANFDTKNTRGSTSFHIASKNGRLEVVKLLIDNGANVDTTNNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNRAN-------------VDTTDNEGWTPLHYA-------------SRNGHLEVVKLLIDNGANVDTKNTR---GSTSFHIASKNGRLEVVKLLIDNGANVDTTNNEGWTPLHYA-----SR------NGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQ 820 HSP 2 Score: 89.3521 bits (220), Expect = 2.726e-14 Identity = 134/596 (22.48%), Postives = 242/596 (40.60%), Query Frame = 2
Query: 125 RTLRLEFVNKLIDDK---------GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF--HTENL-IAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR-------------PVENKEINDLMK--EITEENDKLSNLTKE---CILYRSIIHQHAAGFKKFI-NAAVINGPHKIATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSAS 1807
R +L+ V LID++ G P+H A G L +V LL++ N++ G+ P+HYA+ NG +++VKLL+ ++ +D G TP A + + ++L + N+ A + P+ + + D + G T L A+ + +++K +++ P+ N +K ++ EN + T+ L+ + + H K I N A ++ + + SF I ++ G +L ID + W + A E+ + + + F+++ T H A + ++VK ++D+ N+D +NNE T L A H + +K+L +T+N T H +G + LI+ N+D G T LH A G V + +++NGAN F S + ++ L L++N + TT+N + LH A + E+ K L+D+GA VD N+ G++ + N R+ E+ +LI GA+++ R T+ AS
Sbjct: 305 RNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQN--EGCTPLHYASRNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNANVDTTQNE---GCTPLHYASRNGNLELVKLLIENRANVDTAQNEGWTPLHYSSQNGHLKVVKLLIENKANVDTTQNEGWTPLHYAFQNGHLEVVKFLIDNGANVDTMNTRGSTSF-HIVSQNGRLVLVKLLIDNRANVDTTDNEGWT-PLHYASQNGHLEVVKFLIDNGANFDTKNTRGSTSFHIASKNGRLEVVKLLIDNGANVDT-TNNEGW-TPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFHIVSQNGRLEVVKLLIDNRANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTKNTRGSTSFHIASKNGRL------EVVKL-LIDNGANV-----------------DTTNNE----GWTPLHYASRNGHLEVVKLLIDNGANVDTKNARGSTSFH--IVSQNGRL---------EVVKLLIDNGANVDTTYNERWTLLHDAS 852 HSP 3 Score: 70.4774 bits (171), Expect = 1.630e-8 Identity = 32/90 (35.56%), Postives = 55/90 (61.11%), Query Frame = 2
Query: 164 DKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
++G P+H A E G L++V LL++ N++ G+ P+HYA+ NGN+E+VKLL+ ++ +D T + G TP A + + D ++L
Sbjct: 96 NEGCTPLHKAAENGHLDVVKLLIDNKANVDTAQSEGWTPLHYASRNGNLELVKLLIDNRANVD--TAQYEGWTPLHYASRNGQLDVVKLL 183 HSP 4 Score: 69.707 bits (169), Expect = 3.571e-8 Identity = 33/86 (38.37%), Postives = 53/86 (61.63%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRID-CRTKGWFGLTPFDIAKKWSRTDCIELF 433
P+H A KG + +V LL++ N NI+ +D G P+HYA+ NGN+E+VKLL+ ++ +D + +GW TP A + D ++L
Sbjct: 2 PLHTAAGKGNIEMVKLLIDHNANIDTKDDEGCTPLHYASRNGNLEMVKLLIDNRANVDTTQNEGW---TPLHYASQNGHIDVVKLL 84 HSP 5 Score: 69.3218 bits (168), Expect = 4.272e-8 Identity = 37/108 (34.26%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 137 LEFVNKLIDDK---------GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
LE V LID++ G P+H A + G L++V LL++ N++ G P+HYA+ NGN+E+VKLL+ ++ +D T + G TP A + + D ++L
Sbjct: 210 LELVKLLIDNRANVDTAQYEGWTPLHYASQNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVD--TAQYEGWTPLHYASRNGQLDVVKLL 315 HSP 6 Score: 68.9366 bits (167), Expect = 5.517e-8 Identity = 38/112 (33.93%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 125 RTLRLEFVNKLIDDK---------GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
R LE V LID++ G P+H A G L++V LL++ N++ G P+HYA+ NGN+E+VKLL+ ++ +D T + G TP A + + D ++L
Sbjct: 272 RNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASRNGNLELVKLLIDNRANVD--TAQYEGWTPLHYASQNGQLDVVKLL 381 HSP 7 Score: 68.9366 bits (167), Expect = 5.957e-8 Identity = 38/112 (33.93%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 125 RTLRLEFVNKLIDDK---------GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
R LE V LID++ G P+H A G L++V LL++ N++ G P+HYA+ NGN+E+VKLL+ ++ +D T + G TP A + + D ++L
Sbjct: 140 RNGNLELVKLLIDNRANVDTAQYEGWTPLHYASRNGQLDVVKLLIDNRANVDTTQNEGCTPLHYASQNGNLELVKLLIDNRANVD--TAQYEGWTPLHYASQNGQLDVVKLL 249 HSP 8 Score: 63.929 bits (154), Expect = 1.962e-6 Identity = 31/91 (34.07%), Postives = 51/91 (56.04%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
DD+G P+H A G L +V LL++ N++ G+ P+HYA+ NG+I++VKLL+ ++ +D G TP A + D ++L
Sbjct: 29 DDEGCTPLHYASRNGNLEMVKLLIDNRANVDTTQNEGWTPLHYASQNGHIDVVKLLIDNRANVDTTQN--EGCTPLHKAAENGHLDVVKLL 117
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Match: J9LAT2 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2) HSP 1 Score: 103.605 bits (257), Expect = 2.208e-18 Identity = 138/571 (24.17%), Postives = 232/571 (40.63%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVI----NGPHKIATQSFL---EIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXX---XXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLAL 1840
+HIA +K LLL + N + G+ P+H A+ GN I KLLL ++ K +TP +A KW +++ + L L +S + K GLT L AA S D++I +L R N +S+ TK + + +H A G + AA + N P T +L +AA GH +++K + + K ++ E + + E+ + T LH A ++V ++L H N DL + G T L LA + I++L + + + + QTPLH A G D L+ +D T LH A +G E V ++ N A+ + F S I + +K + +N +T L + H + L LDK S + A++G ++ LH+A K Q ++ +LLD GAK + + G +PL ++ ++ N +E+T +L+ + AD N KS N +T ++ + ++ + L
Sbjct: 180 LHIAAKKDDCKAADLLLQNDHNPDVTSKSGFTPLHIASHYGNDGIAKLLLAKGADVNYSAK--HNITPLHVAAKWGKSNMVSL--------------------LLESGANIEAKTRDGLTALHCAARSGHDQVIDMLLQR-----------------NAPISSKTKNGL---AALHMAAQG--DHVEAAKVLLSNNAPVDDVTVDYLTGLHVAAHCGHIRVAKLLLEKHADPDARALNGFTPLHIACKKNRIKVVELLLKYNASLEATTESGLTPLHVASFMGCMNIVIFLLQHEANPDLPT---VRGETPLHLAARANQTDIIRILLRNGAQVDARAREKQTPLHIASRLGNVDIVMLLLAHGAAVDSTTKDLYTALHIASKEGQEEVASVLLENEASVTATTKKGFTPLHLASKYGNIKVTKLLLQKQAPVDAQGKNGVTPLHVASHYDNQAVALMLLDKQASPHATAKNG--HTPLHIAAKKNQMDIAVTLLDYGAKANAESKAGFTPL---------HLSSQEGN--VEMTTLLLNHNADPNYKSKNGLTPMHLTAQEDKHKVAVVL 690 HSP 2 Score: 62.003 bits (149), Expect = 9.971e-6 Identity = 33/88 (37.50%), Postives = 48/88 (54.55%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+HIAC+K + +V LLL N ++E G P+H A+ G + IV LLQH+ D T G TP +A + ++TD I +
Sbjct: 374 GFTPLHIACKKNRIKVVELLLKYNASLEATTESGLTPLHVASFMGCMNIVIFLLQHEANPDLPT--VRGETPLHLAARANQTDIIRIL 459
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44]) HSP 1 Score: 96.2857 bits (238), Expect = 1.122e-19 Identity = 140/601 (23.29%), Postives = 232/601 (38.60%), Query Frame = 2
Query: 137 LEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL-IAAIKKN--DPIEFEKLCKSYDFMSQKLHTG----------LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKEC----ILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLS--IKFH-----GKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
L+ V I G P+HIA LN+ LLLN N+ G P+H A+ GN+ +V+LLL ID +TK LTP A + IE+ + I A KN PI D + Q L LT L AA ++ K +LD+ K T + + L C + ++ +H+A + + + + L +A+ GH + +++K + V+ + A E+ E + + +++ K + T LH A ++VK +L+H N +S T L +A H + +++L K TPLH A G D L+ R N + G GLT LH AV +V +V+ G + + S +N++ + S++ NA +L H G+ + L L S +N GN + L LH+ + + L+ GA V G +PL + C+ + I++ L+ A++N K+ T A++ + I+ LL +
Sbjct: 167 LKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDE--LTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGA---------KPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGL----------TPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANP--NSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEV--ASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSL--LISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPL-HVACHYGN----------IKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHG 729 HSP 2 Score: 71.2478 bits (173), Expect = 5.030e-12 Identity = 115/537 (21.42%), Postives = 197/537 (36.69%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAK-------------KWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAA------------------GFKKFINAAVINGPHK-------IATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
P+H A G + I+ +LL++ I+ + G PIH A +++ V+ LLQ+ ID T LTP +A K ++ + L L A KKN + L K + +GLT L A+ I+K +L + N + E +++ C + ++ A G K+ + + HK A + L IAA +GH + +D ++T K + + A ++ E + ER + K T LH A+ N+ D+V ++ + + N T L +A + + L QY + N ++ TPLH A G D LI++ N++ GLT LH +G + + +V GA+ + + I + +KF L + + R G + LH A + ++ LL GA+ + + +NG S L
Sbjct: 247 PLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH--LTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKV----ETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLL---EHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFT-PLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGY--TPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKM-------------------VKF-------LLQQQANVNGKTRAGY--TPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSAL 743 HSP 3 Score: 67.781 bits (164), Expect = 5.937e-11 Identity = 130/615 (21.14%), Postives = 230/615 (37.40%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTK----------------------------------GWFGLTPFDIAKKWSRTDCIEL---------FHTENLIA----AIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENE---ITIPFEKSIIALSLMENALT---LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
KG+ +HIA G +V L+N N+ + G+ P++ A ++E+VK LL++ T+ G TP IA + + +L F +N I A ++ + I L + K LT L AA + +II+ +LD+ P++ K N L + + D + +C+ R ++ +A I+ ++ + L +AA GH M K + +K + T +N FT LH A N ++ +L H + L++ E+ T L +A + H +K+L Q + N N +TPLH A +G + +L+ + +D T LHCA G + + + ++ + AN + E + I + + + + T ++ K+ GK+ + L NA N + LH+AV ++ L+ G NG +PL K N+ +E+ + L+ YGA N +S +T A++ E M++LL
Sbjct: 75 KGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHM-----DCV--RQLLQYNAE-----IDDITLD------HLTPLHVAAHCGHHR-----MAKVLLDKG-----------------AKPNTRALN--------GFTPLHIACKKNHLRVMDLLLKH--SASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKY-GKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLH----------IASKQNQ-VEVASSLLQYGASANAESLQGVTPLHLAAQ-EGRADMVSLL 626
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 93.9745 bits (232), Expect = 6.048e-19 Identity = 140/592 (23.65%), Postives = 228/592 (38.51%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIE-LFHTENLIAAIKKN--DPIEFEKLCKSYDFMSQKLH----------TGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLME-NALTLSIKFHGKLFLCFLDKLNS----TTSNARDGNNN-------SLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMT------EEKVNRMIEIT----------NILIGYGADINVKSTNRITIFQSASKL 1813
G P+HIA G +N+ LLLN ++ + P+H A+ GN +VKLLL+ RID +TK GLTP + +E L I + KN P+ + + LH LT L AA K+ K I+D K+ N K + K I ++ +H A + + + + + +AA GHE + +M + VR A+ A E+ + + + +++ K + T LH + ++++ +L H DS + T L LA H+ +L + K TPLH A G + L+ + D G GLT LH A + V +++ GA+ + + + +N++ IA +L+E A T +I G L + + T ARD N + LH+A + Q + + L++ GA VD G +PL N +M + KVN ++T N+L+ +GA N + N T A +L
Sbjct: 182 GFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKD--GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVD-----KKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGL----------TPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQH--GACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQME-------IATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVTAAQQGHTHIINLLLQHGAAPNELTVNGNTALSIARRL 747 HSP 2 Score: 88.1965 bits (217), Expect = 3.835e-17 Identity = 115/510 (22.55%), Postives = 206/510 (40.39%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRA---IXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDH---PNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
+HIA K LLL + N + E G+ P+H A GNI + LLL +D + + +TP +A K ++ ++L E+ + + K GLT L A S +++++ +LDR P+ +K N L + + D L+ C+ ++ H N + + L +AA GH I+ K + + + A K ++ E + + ++ + T +H A ++V +++H PN ++ TAL +A + +K L Q + ++K QTPLH + G + L+ D G T LH A +G ++V +++ GA+ + F + +I + +K + ++ LT L + H K+ L LD+ S + A++G + LH+A K Q E+ +LL+ GA + + G SPL
Sbjct: 153 LHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARN--DITPLHVASKRGNSNMVKLL----------------LERGAR----IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLN-----CV---QLLLHHEVPVDDVTNDYL----------TALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGE-----TALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNG--YTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPL 615 HSP 3 Score: 58.9214 bits (141), Expect = 3.074e-8 Identity = 77/325 (23.69%), Postives = 133/325 (40.92%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSE-NEITIPFEKSIIALSLMENALT-LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL-FEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
+++ KV LH A +DT +L + +N D++S + T L +A + +L A + + + TPLH A G + L+ R ID GLT LHC G E V E +++ GA + + L+ L Q N + + + + + LT L + H K+ +DK NA+ N + LH+A K + ++ + LL GA + V +G +P+ F + +T+ L+ +GA N + T A++ ++ L+ N + + SKD Q
Sbjct: 143 DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGF--TPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK--KANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQ------------LMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNG-AYVDAKSKDDQTPL 450 HSP 4 Score: 54.6842 bits (130), Expect = 5.550e-7 Identity = 79/347 (22.77%), Postives = 142/347 (40.92%), Query Frame = 2
Query: 911 IQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILD-----------------------HPNNIDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
++E +T N+ ++ + FT L+ A +N D+V+Y+LD H + L N+T G AL +A K +L Q + + ++K TPLH A G + L+NR +D +T LH A +G N+ + ++ GA + C + E + + ++ LS +N L+ L + G C L+ D N+ L LH+A C ++ K ++D A + NG +PL + C N I++ +L+ +GA I + + +T A+ + +E I+ L+++
Sbjct: 70 VKELVTNGANV-NAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVP-VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPL-HIACKKNR----------IKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHG 403 HSP 5 Score: 52.373 bits (124), Expect = 2.792e-6 Identity = 21/70 (30.00%), Postives = 43/70 (61.43%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTK 364
L + G P+H+A ++ +N+ +L+N ++ E GY P+H A GN+++V LL+++ +++ +TK
Sbjct: 639 LCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTK 708
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6]) HSP 1 Score: 88.9669 bits (219), Expect = 1.617e-17 Identity = 126/510 (24.71%), Postives = 203/510 (39.80%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNF--LQFTCQSSENEIT-IPFEKSIIALSLMENALT-LSIKFHG---KLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
++ G P+HIA L++ LLLN N+ G P+H A GN+ +V+LLL +ID RTK LTP A + IE+ + + PI+ K GL+ + AA + ++ +L N EI+D IT ++ ++ C +R G K NA +NG + L IA ++ H +D ++K ++ S+ E +TE T LH A ++VK +L +++N + T L +A H + + L Q + + K QTPLH A G + L+ N D G T LH A +G + +++ A K + F L C+ + ++ + E+ + +N LT L + H + + K S S AR+G + LH+A K Q E+ SLL GA + + G +PL
Sbjct: 176 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDE--LTPLHCAARNGHVRVIEIL--------LDQGAPIQ------------AKTKNGLSPIHMAAQGDHMDCVRQLLQY---NAEIDD----ITLDHLTPLHVAAHCGHHRMAKVLLDKGAKA--NARALNG------FTPLHIACKKNHMRSMDLLLK---HSASL-----------------EAVTES----------GLTPLHVAAFMGHLNIVKSLLQ--RGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNG--YTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPL 614 HSP 2 Score: 60.4622 bits (145), Expect = 1.009e-8 Identity = 26/85 (30.59%), Postives = 46/85 (54.12%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
P+H+ ++G + I +L+ + ++ GY +H A GNI++VK LLQ + ++C+T+ G TP A + TD + L
Sbjct: 651 PLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTR--VGYTPLHQAAQQGHTDIVTLL 733 HSP 3 Score: 56.6102 bits (135), Expect = 1.304e-7 Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+ +H+AC G + +V LL + ++ C+ GY P+H A G+ +IV LLL+H + T G +P IAK+ I++
Sbjct: 681 GYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTN--GTSPLGIAKRLGYISVIDVL 766
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 86.6557 bits (213), Expect = 9.175e-17 Identity = 133/586 (22.70%), Postives = 230/586 (39.25%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAI---KKNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLS--IKFH-----GKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
G P+HIA G +N+ LLLN ++ P+H A GN +VKLLL +ID +TK GLTP + +E+ T+N ++ + + D + +L +D + LT L AA K+ K I+D K+ N K + K + ++ +H A + + + + + +AA GHE + + + VR A+ A ++ + + + + K + T LH A ++V+ +L S T G T L LA H++ +L ++ + + K TPLH A G + L+ + D G GLT LH A + V ++++GA+ + + + +N++ I +++ NA+T H G + L L L + +N GN N L LH+A + + + + LL+ GA+VD G +PL N +M + L+ A IN K+ + T A++ + I+ LL +
Sbjct: 237 GFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKD--GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVD-----KKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGL----------TPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLL---QKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEI--GTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSL--LLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKM-----------VSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQH 787 HSP 2 Score: 84.7297 bits (208), Expect = 3.751e-16 Identity = 127/595 (21.34%), Postives = 225/595 (37.82%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINID--------------------------QIGFL-------GLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLA 1837
+HIA K LLL + N + E G+ P+H A GNI + LLL +D + +TP +A K + ++L ++ K + K GLT L A S +++++ +LDR P+ +K N L + + D L+ C+ ++ QH N + + L +AA GH ++ + I ++ ++ FT LH A N +++ +L H L + E+ T + +A + H +K L + + NT N +T LH A +G D YL+ +D Q G G T LH AV +G + ++ +GA+ + F + +I +K + + LT L + H ++ L LD S + A++G + LH+A K Q E+ +LL+ GA+ + V G SPL + S +++ ++L+ A++N+ + N +T A+ + +R+ +A
Sbjct: 208 LHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARN--DITPLHVAAKRGNGNMVKLL----------------LDRGAK----IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLN-----CV---QLLLQHDVPVDDVTNDYL----------TALHVAAHCGH------------------------------YKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKH--GASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNG--YTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPL-HLAAQEGS----------VDLVSLLLTKNANVNMGNKNGLTPLHLAA--QEDRVGVA 715 HSP 3 Score: 58.5362 bits (140), Expect = 3.457e-8 Identity = 79/324 (24.38%), Postives = 133/324 (41.05%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSE-NEITIPFEKSIIALSLMENALT-LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
+++ KV LH A +DT +L + +N D++S + T L +A + +L A + + TPLH A G + L++R ID GLT LHC G E V E +++ GA + + L+ L Q N + + + + + + LT L + H K+ +DK NA+ N + LH+A K + ++ + LL GA + V +G +P+ + + M E I L +GA N + T A++ ++ LL N + +KD Q A
Sbjct: 198 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGF--TPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK--KANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPI-----HVAAFMGHEN------IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKV-DIKAKDDQTAL 505 HSP 4 Score: 54.6842 bits (130), Expect = 5.883e-7 Identity = 25/80 (31.25%), Postives = 44/80 (55.00%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIA 397
+ +G P+H+A ++G +++V LLL +N N+ + G P+H A + + ++LL H +D TK G TP +A
Sbjct: 662 VTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTK--TGYTPLHVA 739 HSP 5 Score: 54.6842 bits (130), Expect = 6.086e-7 Identity = 32/98 (32.65%), Postives = 47/98 (47.96%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW---SRTDCIELFHTENLIA 454
G+ P+H+AC G + +V LL I + GY P+H A G+ I+ +LLQH+ + T G T IA++ S D + ENL A
Sbjct: 732 GYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELT--LNGNTALSIARRLGYISVVDTLRGLTDENLTA 827
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 86.2705 bits (212), Expect = 1.465e-16 Identity = 133/586 (22.70%), Postives = 230/586 (39.25%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF---------HTENLIAAIK---KNDPIEFEKLCKSYDFMSQKLHTG-LTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG-TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLS--IKFH-----GKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN 1849
G P+HIA G +N+ LLLN ++ P+H A GN +VKLLL +ID +TK GLTP + +E+ T+N ++ + + D + +L +D + LT L AA K+ K I+D K+ N K + K + ++ +H A + + + + + +AA GHE + + + VR A+ A ++ + + + + K + T LH A ++V+ +L S T G T L LA H++ +L ++ + + K TPLH A G + L+ + D G GLT LH A + V ++++GA+ + + + +N++ I +++ NA+T H G + L L L + +N GN N L LH+A + + + + LL+ GA+VD G +PL N +M + L+ A IN K+ + T A++ + I+ LL +
Sbjct: 209 GFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKD--GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVD-----KKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGL----------TPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLL---QKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEI--GTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSL--LLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKM-----------VSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQH 759 HSP 2 Score: 57.7658 bits (138), Expect = 6.372e-8 Identity = 80/330 (24.24%), Postives = 133/330 (40.30%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLG------TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE--LNFLQFTCQSSE-NEITIPFEKSIIALSLMENALT-LSIKF---HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAF 1897
+++ KV LH A +DT +L + +N D++S T T L +A + +L A + + TPLH A G + L++R ID GLT LHC G E V E +++ GA + + L+ L Q N + + + + + + LT L + H K+ +DK NA+ N + LH+A K + ++ + LL GA + V +G +P+ + + M E I L +GA N + T A++ ++ LL N + +KD Q A
Sbjct: 162 DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK--KANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPI-----HVAAFMGHEN------IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKV-DIKAKDDQTAL 477 HSP 3 Score: 54.6842 bits (130), Expect = 6.689e-7 Identity = 25/80 (31.25%), Postives = 44/80 (55.00%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIA 397
+ +G P+H+A ++G +++V LLL +N N+ + G P+H A + + ++LL H +D TK G TP +A
Sbjct: 634 VTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTK--TGYTPLHVA 711 HSP 4 Score: 54.299 bits (129), Expect = 7.157e-7 Identity = 32/98 (32.65%), Postives = 47/98 (47.96%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW---SRTDCIELFHTENLIA 454
G+ P+H+AC G + +V LL I + GY P+H A G+ I+ +LLQH+ + T G T IA++ S D + ENL A
Sbjct: 704 GYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELT--LNGNTALSIARRLGYISVVDTLRGLTDENLTA 799
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.0333 bits (201), Expect = 3.496e-16 Identity = 133/571 (23.29%), Postives = 228/571 (39.93%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFID--GIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEI---TIPFEKSIIALSLM--ENALT---LSIKF-HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
D KG P +HIA K N LLL N + G+ P+H A GN+ + LLL +D + G+TP +A K + ++L ++ K + K GLT L AA S +K+I+ +L+R P+ K N L + + + L+ C+ Q G + ++ ++ + L +AA GH +L +D G N + A K ++ E + + + + T +H A ++V+ +L N ++ N T L +A + +K L Q + + QTPLH A G + L+ R + + G T L+ A +G +V ++ NGA+ + F S + T+ EK A + +N LT ++ + H K+ L LDK S S A++G + LH+A K Q E+ K LL+ GA+ +++ G +PL ++ M +L+G A I++ + +T A++
Sbjct: 166 DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARN--GITPLHVAAKRGNNNMVKLL----------------LDRGAK----IDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLA-----CV-------QLLLGRQAPVDDVTLD------YLTPLHVAAHCGHYRVAKLLLDRGGEPNARALN---GFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQ--NGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNG--YTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVA-----------LLLGRHASISLATKGGLTAMHLAAQ 678 HSP 2 Score: 68.9366 bits (167), Expect = 4.671e-12 Identity = 83/314 (26.43%), Postives = 128/314 (40.76%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSE---NEITIPFEKSIIALSLMENALTL--SIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDF 1885
T LH A + +MVK +LD ID + + T L A H K I++L + + + K +PLH A L+ R +D + LT LH A G V + +++ G N L C+ + E+ + SI + E+ LT F G L + L N +SNA + + LHMA + Q E+ K+LL SGA+V V +PL + SR+ + EI +L+ G+D N + + T AS+ + + LL N + K F
Sbjct: 238 GITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGL--TPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASI--HTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPL-----HIASRLGKA------EIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGF 536 HSP 3 Score: 62.7734 bits (151), Expect = 3.183e-10 Identity = 132/604 (21.85%), Postives = 222/604 (36.75%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIE-LFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTG------------LTILQTAADSNADKIIKCILDRPVEN--KEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRA---IXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQ--IGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIF----KNFSSKDFQMAFDNH 1906
G P+HIA G +N+ LLLN ++ G P+H A GN +VKLLL +ID +T+ GLTP A + IE L + I A KN + +L G LT L AA ++ K +LDR E + +N I + +++ + E +L +H A I+ +G I SF+ GH + +++ + + VR + A E+ + + + + + + T LH A ++V+ +L D ++ + T L A H +L + + K TPLH A G + L+ + + D G GLT LH A + V +++ GA+ + + + +N++ + GK+ L + N T + LH+A + ++ LL A + + G L M + E+KVN + IL + AD+N + T AS N +++ LL N + +N + Q A H
Sbjct: 205 GFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRD--GLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVM--ELLL------KHGAS----IHTTTESGLTPIHVASFM------GHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQ--RGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEV-----------------------GKVLLELGAETNMLTQQGV-----APLHLAAQEGHADMVALLLGRHASISLATKGG---LTAMHLAAQ----EDKVN----VAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGH 747 HSP 4 Score: 59.6918 bits (143), Expect = 2.569e-9 Identity = 74/302 (24.50%), Postives = 121/302 (40.07%), Query Frame = 2
Query: 947 ESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNN 1852
+++ KV LH A +DT+ +L H +N D+ S + T L +A + +L A + + TPLH A G ++ L++R ID GLT LHCA G E V E ++ A + + N L +++ E + + L L A + LD L LH+A C + K LLD G + + NG +PL + C N I++ +L+ +GA I+ + + +T AS + + ++ LL N
Sbjct: 166 DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGF--TPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTK-NGLSPLHMATQGE-----HLACVQLLLGRQAPVDDVT---------LDYLTP-------------LHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPL-HIACKKNR----------IKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNG 426 HSP 5 Score: 48.521 bits (114), Expect = 7.489e-6 Identity = 32/164 (19.51%), Postives = 63/164 (38.41%), Query Frame = 2
Query: 131 LRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEI 622
L L ++ +G P+H+A ++G ++V LLL + +I G +H A + + ++L +HK ++ TK + TP +A + + L ++ ++ K G T L AA ++ +L E+
Sbjct: 623 LELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCY--TPLHVASHYGNIKMVNF--------------------LLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNEL 764
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 78.9518 bits (193), Expect = 4.060e-15 Identity = 133/577 (23.05%), Postives = 227/577 (39.34%), Query Frame = 2
Query: 161 DDKG--HFP-IHIACEKGFLNIVILLLNENCNIECEDIW--------GYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFID--GIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEI---TIPFEKSIIALSLMENALT---LSIKF-HGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASK 1810
D KG P +HIA K N LLL N + G+ P+H A GN+ + LLL +D + G+TP +A K + ++L ++ K + K GLT L AA S +K+I+ +L+R P+ K N L + + + L+ C+ Q G + ++ ++ + L +AA GH +L +D G N + A K ++ E + + + + T +H A ++V+ +L N ++ N T L +A + +K L Q + + QTPLH A G + L+ R + + G T L+ A +G +V ++ NGA+ + F S + T+ EK A +N LT ++ + H K+ L LDK S S A++G + LH+A K Q E+ K LL+ GA+ +++ G +PL ++ M +L+G A I++ + +T A++
Sbjct: 165 DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARN--GITPLHVAAKRGNNNMVKLL----------------LDRGAK----IDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLA-----CV-------QLLLGRQAPVDDVTLD------YLTPLHVAAHCGHYRVAKLLLDRGGEPNARALN---GFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQ--NGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGY--TPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVA-----------LLLGRHASISLATKGGLTAMHLAAQ 683 HSP 2 Score: 68.5514 bits (166), Expect = 5.719e-12 Identity = 83/314 (26.43%), Postives = 128/314 (40.76%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGA--NYMKFVELNFLQFTCQSSE---NEITIPFEKSIIALSLMENALTL--SIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDF 1885
T LH A + +MVK +LD ID + + T L A H K I++L + + + K +PLH A L+ R +D + LT LH A G V + +++ G N L C+ + E+ + SI + E+ LT F G L + L N +SNA + + LHMA + Q E+ K+LL SGA+V V +PL + SR+ + EI +L+ G+D N + + T AS+ + + LL N + K F
Sbjct: 245 GITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGL--TPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIH--TTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPL-----HIASRLGKA------EIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGF 543 HSP 3 Score: 48.521 bits (114), Expect = 8.163e-6 Identity = 32/164 (19.51%), Postives = 63/164 (38.41%), Query Frame = 2
Query: 131 LRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEI 622
L L ++ +G P+H+A ++G ++V LLL + +I G +H A + + ++L +HK ++ TK + TP +A + + L ++ ++ K G T L AA ++ +L E+
Sbjct: 628 LELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCY--TPLHVASHYGNIKMVNF--------------------LLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNEL 769
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009608.1 (pep scaffold:Pmarinus_7.0:GL477242:23155:61557:-1 gene:ENSPMAG00000008655.1 transcript:ENSPMAT00000009608.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.0922 bits (170), Expect = 2.358e-12 Identity = 136/633 (21.48%), Postives = 244/633 (38.55%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCR---IDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPV-ENKEIND-LMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYI--LDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTS-NARD--GNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNL 2011
D G P+H AC G + LL+ ++ D+W + P+H A +E+ LLL + ++C +K L P K+ + F +L+ A ++ D +K H GL I+ + + C + P + K++++ L+++ N+K +L + H + A IN + Q+ L AA GH L + + + + A + E ++I +L V +L +A D D VK + N D++ + T L A + ++ L Q+ + ++K PLH A + G + L+ R ++ T LH A KG +C+ ++ +GA+ K + ++ ++ + I L + AL + K K L + KL S + N RD G N++ LH+A E+ + LL+ GA V+ + G PL + ++I +LI Y A +N T A++ ++ LL++ +P KN ++ Q D A+ + LL D+ + EA PN+
Sbjct: 364 DKGGLVPLHNACSYGHYEVTELLVKHGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHSKSAIDLAPTSELKER----LVYEFRGHSLLQAAREADVTRVKK------------HLGLDIINFKHPQTHETALHCAVASPYPKRKQVSELLLRKGANVNEKTKDLLTPLHVASDKAHNDVIDV-LLKHGAKINVTDSLG-QTSLHRAAHAGHLTTCRLLLSHGCDPNLPSLQGFTAA------QLGNESVQQILHESSLPVRNSDVEHQLLEAAKA-GDLDTVKQLCTTQTVNCRDMEGRHS---TPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTK-------KNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAK---KGCLARVQKLCSGENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGH-----------VDIAALLIKYSACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPSMKN---QEGQTPLD---LATADDVRALLIDA-----MPPEALPVTHKPPNI 936 HSP 2 Score: 59.3066 bits (142), Expect = 4.869e-9 Identity = 41/152 (26.97%), Postives = 66/152 (43.42%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKL-------CKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL 595
DD G P+H AC G +V LLL + + D W Y P+H A + G I++ +LLQH D + G T D+A+ ++ + + L+ A + + + L C + D T L AA N +I++ +L
Sbjct: 211 DDGGLIPLHNACSFGHAEVVNLLLRQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGA--DPGIRNTDGKTALDLAEPTAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKS------TPLHLAAGYNRVRIVQLLL 354
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 6.139e-11 Identity = 109/537 (20.30%), Postives = 213/537 (39.66%), Query Frame = 2
Query: 269 GYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTL---------SIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
G P+H A +G+ ++V++LL+ I +TK GL+P +A + +C+++ ++ P++ L D+ LT L AA + K +LD+ N + L + + L C R + + + I A +G + +F+ G+ + +++ + VR A+ A E+ + +++ + T LH A ++V+ +L H + D ++N T L +A H +L ++ + K TPLH A G + L+ R + D G GLT LH A + V +++ GA+ + + + ++++ I +++ NA+T + + H + L + + G S LH+A + + + + L+ GA V+ G +PL + C+ + I++ N L+ +GAD+N K+ N T A++ + I+ LL
Sbjct: 6 GLTPLHCAARSGHEQVVEILLEQGAPILAKTKN--GLSPLHMATQGDHVECVQVL--------MQYRAPVDDITL----DY--------LTALHVAAHCGHYRTAKVLLDKGA-NPNVRAL--------NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFM------GNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGY--TPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEI--ATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSG--LSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLI-VACHYGN----------IKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488 HSP 2 Score: 59.3066 bits (142), Expect = 4.877e-9 Identity = 69/307 (22.48%), Postives = 115/307 (37.46%), Query Frame = 2
Query: 965 NFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDF 1885
T LH A +V+ +L+ I + N + L +A H +C++VL QY + T LH A G L+++ N + G T LH A K V E ++ GA+ E P S F G L + + + NA + + LHMA + Q E+ +SLL +GA+VD +PL + +R+ + +EI +L+ + A + ++N T A++ +E + LL + + K F
Sbjct: 6 GLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGL--SPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGL-------------TPMHVS---------------AFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPL-----HIAARLGK------LEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGF 271 HSP 3 Score: 56.9954 bits (136), Expect = 2.339e-8 Identity = 36/113 (31.86%), Postives = 53/113 (46.90%), Query Frame = 2
Query: 104 GKTEELRRTLRLEFVNKLIDDKG---HFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+TE +R LR +D K P+HIA G L IV LLL + + GY P+H A G+ ++ +LL+H + TK G TP +A K+ + + L
Sbjct: 182 GQTEVVRSLLR---NGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKK--GFTPLHVASKYGKMEVASLL 289 HSP 4 Score: 51.6026 bits (122), Expect = 9.487e-7 Identity = 24/79 (30.38%), Postives = 40/79 (50.63%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKW 406
G+ P+H+A +G ++ +LL ++ G+ P+H A+ G +E+ LLLQ D K GLTP +A +
Sbjct: 237 GYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKN--GLTPLHVASHY 313 HSP 5 Score: 50.447 bits (119), Expect = 2.086e-6 Identity = 43/158 (27.22%), Postives = 63/158 (39.87%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTD--CIELFHTENLIAAIKKN-DPIEFEKLCKSYDFMSQKLHT----------GLTILQTAADSNADKIIKCILDR 601
+G +H+A G +V LL ++ + P+H A G +EIV+LLLQH D T G TP +A + D I L H +L KK P+ + S L GLT L A+ + K+ +LD+
Sbjct: 170 RGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSN--GYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 325 HSP 6 Score: 49.2914 bits (116), Expect = 4.703e-6 Identity = 24/92 (26.09%), Postives = 48/92 (52.17%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ + G P+H++ G LNIV++LL + ++ G +H A G E+V+ LL++ ++D + + TP IA + + + ++L
Sbjct: 134 VTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREE--QTPLHIAARLGKLEIVQLL 223 HSP 7 Score: 48.9062 bits (115), Expect = 6.273e-6 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G P+H+A ++ +N+ +L+ ++ GY P+ A GNI++V LLQH ++ +TK G TP A + T + L
Sbjct: 402 GLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKN--GYTPLHQAAQQGHTHIVNLL 487
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 57.7658 bits (138), Expect = 1.283e-8 Identity = 58/238 (24.37%), Postives = 99/238 (41.60%), Query Frame = 2
Query: 959 KVNFTILHSAICDNDTDMVKYILDHPNNID-LDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNF--LQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
+ T LH A + ++MV +++ NI+ D + A+ A + H + +KVL + ++K +PLH A +SG L++ ++ID G T LH A G + V ++++ GAN + E F L F S+ HG L L L N N + + S LHM + ++L+ +G ++D + +GN+PL
Sbjct: 136 RAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRR---AIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAAST----------------------------HGALCLELLVN-NGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPL 341 HSP 2 Score: 52.373 bits (124), Expect = 6.262e-7 Identity = 80/362 (22.10%), Postives = 152/362 (41.99%), Query Frame = 2
Query: 914 QEEITERMNLFESRIKVN----FTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIAL--------SLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLL-DSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQ---YAECLLKLLS 1945
+ EI E + L +R+ T LH A V+ +L H + D+++ ++ T L +A + KC + + ++N ++ +T LH A +G + +L+ + NI+ +H A G V + +V +GA+ + + SS +I + K ++ L S AL ++ ++G+ + +++L +N N LH A LC LL ++GA V+V + +G SPL MT V+ + LI G +I+ + + T A++ +E ++ L++N + + + F H +++C KLLS
Sbjct: 51 EAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKH--SADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGV--VKLLLDLGVDIDDPNSFGNTALHVAC-YNGQDVV--VNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPL---------HMT--AVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNG---ADTAKRGIHGMFPLHLAALSGFSDCCRKLLS 391 HSP 3 Score: 48.521 bits (114), Expect = 8.764e-6 Identity = 28/79 (35.44%), Postives = 39/79 (49.37%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIA 397
D K IH A G +V +L+ ++ C+D GY P+H A+ +G I +VKLLL ID FG T +A
Sbjct: 168 DKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDID--DPNSFGNTALHVA 244
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 50.8322 bits (120), Expect = 1.001e-6 Identity = 26/77 (33.77%), Postives = 44/77 (57.14%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENC-NIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPF 388
D G + IAC+KG ++V ++ E +I +D G +H A L G+IEIV+LL+++ ++ ++ FG TP
Sbjct: 327 DSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPL 403
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Yeast
Match: SPT23 (ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication [Source:SGD;Acc:S000001503]) HSP 1 Score: 48.521 bits (114), Expect = 5.058e-6 Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 2
Query: 92 HLMNGKTEELRRTLRLEFVN-KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWF 373
L+ + +R L VN + D +G +H+A + ++V LL+ ++ +D++G+ P+H A +NG++ I++LLL+ I +T+ F
Sbjct: 683 QLITEVIKSFKRNNNLSTVNLSMCDVRGRTLLHLAAFNNWYSLVSLLIKYGSHLNDQDLFGFTPLHMACINGDLRIIRLLLECNVNIMKKTRNGF 777
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Match: EDO37759 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SET1]) HSP 1 Score: 60.4622 bits (145), Expect = 2.420e-9 Identity = 39/128 (30.47%), Postives = 67/128 (52.34%), Query Frame = 2
Query: 80 MFVSHLMNGKTEELRRTLRLEF---VNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIEC--EDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENL 448
+F S +++G+ + L L LE VNK++ G+ P+H A G ++V LL N ++ ED G P+ A ++GN+ +V LLL CR + G ++P D+AK+ D E+ + ++
Sbjct: 363 LFTS-VISGQEQVLNYLLSLEPRPNVNKVVSASGNTPLHAAVNCGNQSMVKLLANAGADLNAANEDCNGATPLFLAIMSGNLAMVSLLLSLGCRTNVYM-GALAVSPLDLAKELELHDIAEMLDSSHI 488
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Match: EDO44570 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVG1]) HSP 1 Score: 48.521 bits (114), Expect = 1.863e-7 Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQH 337
G P+H AC +G IV +LL +I + GY P+H A L+GN E +++L+ H
Sbjct: 1 GDTPLHFACARGESAIVRVLLLFGADIHARNFAGYSPLHVAALHGNAESIRILILH 56
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Match: EDO30220 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1E6]) HSP 1 Score: 52.373 bits (124), Expect = 7.657e-7 Identity = 28/102 (27.45%), Postives = 52/102 (50.98%), Query Frame = 2
Query: 179 PIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEF 484
P+ +AC G ++V+ L+ + +++ +D G +HY G++EIVK L++ + GLTP +A +T+ +E F +E I + D +E
Sbjct: 24 PLMVACYNGHRDVVMYLVKKGASMDMQDKQGNSVLHYGVERGHLEIVKELVRQGAK---HLPNKLGLTPLLLASNDCKTEMVEYFISEEDCPQIDRIDALEL 122
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Match: EDO45485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSW0]) HSP 1 Score: 46.595 bits (109), Expect = 1.063e-6 Identity = 25/64 (39.06%), Postives = 36/64 (56.25%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRT 361
G P+HIA G IV LLL+E ++E + GY+P+H A G+ I+++LLQ D T
Sbjct: 1 GFTPLHIAVVYGHWEIVKLLLDEGADVEAQTKNGYQPLHLAAQYGHKIIIEILLQRGAPPDALT 64
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35]) HSP 1 Score: 48.1358 bits (113), Expect = 1.839e-6 Identity = 34/144 (23.61%), Postives = 61/144 (42.36%), Query Frame = 2
Query: 182 IHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRT-KGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVE 610
+H A G + LLL + +G++PIH A +NG+ E V++LL++ + +T K +TP A + DC E I+ E + + G ++ A+D+ K++ +LD V+
Sbjct: 1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMER---------GIDLEAVYRD----------GWRLIHVASDNGRVKVLVTLLDAGVD 125
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458]) HSP 1 Score: 85.8853 bits (211), Expect = 1.807e-16 Identity = 129/582 (22.16%), Postives = 233/582 (40.03%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQ 1888
G P+HIA G +N+ LLLN ++ G P+H A+ GN +V+LLL +ID +T+ GLTP A + +E+ ++K P+ L ++ + GL+ L AA + + +K +L PV++ ++ L ++ C YR + + + NA +NG + L IA ++ ++ ++K Y SI + +TE T +H A ++V +L N D +N T+L +A + ++ L + + + + QTPLH A G + L+ + D G T LH + +G ++ GA++ + F S + + ++ S +N LT L + H K+ L LDK S + A++G + LH+A K Q ++ LL GA+ + + G +PL ++ M +L+ GA INV + + +T A+ + +R+ +A + + +N ++ D Q
Sbjct: 226 GFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRD--GLTPLHCAARSGHEASVEVL--------LEKGAPL----LARTKN--------GLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTA---------LHVAAHCGHYR--VTKLLLDKRANPNARALNG------FTPLHIACKKNRVKVMELLVK---YGASI-----------------QAVTES----------GLTPIHVAAFMGHLNIVLLLLQ--NGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNG--YTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAA-----------LLMSKGAQINVPTKSGLTALHLAA--QEDRVAVAEI----LVRNGANLDHQ 717 HSP 2 Score: 68.1662 bits (165), Expect = 4.354e-11 Identity = 95/422 (22.51%), Postives = 168/422 (39.81%), Query Frame = 2
Query: 788 LEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNN-----------------------IDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSK-DFQMAFDNHWFQYAECLLK 1936
L +AA++GH + ++ + S + A+ + E+ + + +R ++ + FT L+ A +N D+V+Y+L++ N + + N+T G AL +A K +L Q + + Q+K TPLH A G + L+NR +D G+T LH A +G N+ +++ G+ C + E + + EK L+ +N L+ L + G C + L + D + L LH+A C + K LLD A + NG +PL + C N +++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 69 LHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVEC-VKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLH-IACKKNR----------VKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLR 478 HSP 3 Score: 60.8474 bits (146), Expect = 8.844e-9 Identity = 30/94 (31.91%), Postives = 50/94 (53.19%), Query Frame = 2
Query: 152 KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ + + G PIH+A G LNIV+LLL + + +I G +H A G E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 418 QAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAR--EDQTPLHIASRLGKTEIVQLL 509
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Match: ENSORLT00000031565.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458]) HSP 1 Score: 85.5001 bits (210), Expect = 2.004e-16 Identity = 129/582 (22.16%), Postives = 233/582 (40.03%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFH---GKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQ 1888
G P+HIA G +N+ LLLN ++ G P+H A+ GN +V+LLL +ID +T+ GLTP A + +E+ ++K P+ L ++ + GL+ L AA + + +K +L PV++ ++ L ++ C YR + + + NA +NG + L IA ++ ++ ++K Y SI + +TE T +H A ++V +L N D +N T+L +A + ++ L + + + + QTPLH A G + L+ + D G T LH + +G ++ GA++ + F S + + ++ S +N LT L + H K+ L LDK S + A++G + LH+A K Q ++ LL GA+ + + G +PL ++ M +L+ GA INV + + +T A+ + +R+ +A + + +N ++ D Q
Sbjct: 241 GFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRD--GLTPLHCAARSGHEASVEVL--------LEKGAPL----LARTKN--------GLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTA---------LHVAAHCGHYR--VTKLLLDKRANPNARALNG------FTPLHIACKKNRVKVMELLVK---YGASI-----------------QAVTES----------GLTPIHVAAFMGHLNIVLLLLQ--NGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNG--YTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAA-----------LLMSKGAQINVPTKSGLTALHLAA--QEDRVAVAEI----LVRNGANLDHQ 732 HSP 2 Score: 68.1662 bits (165), Expect = 4.626e-11 Identity = 95/422 (22.51%), Postives = 168/422 (39.81%), Query Frame = 2
Query: 788 LEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNN-----------------------IDLDSNNETLG----TALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSS---ENEITIPFEKSIIALSLMENALT-LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSL--LHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKNFSSK-DFQMAFDNHWFQYAECLLK 1936
L +AA++GH + ++ + S + A+ + E+ + + +R ++ + FT L+ A +N D+V+Y+L++ N + + N+T G AL +A K +L Q + + Q+K TPLH A G + L+NR +D G+T LH A +G N+ +++ G+ C + E + + EK L+ +N L+ L + G C + L + D + L LH+A C + K LLD A + NG +PL + C N +++ +L+ YGA I + + +T A+ + + I+L LL N +P N + MA + CLL+
Sbjct: 84 LHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVEC-VKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLH-IACKKNR----------VKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLR 493 HSP 3 Score: 60.4622 bits (145), Expect = 9.636e-9 Identity = 30/94 (31.91%), Postives = 50/94 (53.19%), Query Frame = 2
Query: 152 KLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ + + G PIH+A G LNIV+LLL + + +I G +H A G E+V+ LL++ +D R + TP IA + +T+ ++L
Sbjct: 433 QAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAR--EDQTPLHIASRLGKTEIVQLL 524
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380]) HSP 1 Score: 77.411 bits (189), Expect = 6.708e-14 Identity = 130/602 (21.59%), Postives = 214/602 (35.55%), Query Frame = 2
Query: 59 IKMSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFG-------------------------------LTPFDIAK-------------KWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR---------PVENK----------EINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGH----ELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPL 1663
I Y +M V+ L+ L R + F K G P+HIA +G + +V LLL+ I+ + P+H A NG++ I+++LL+H I +TK LTP +A K ++ + L L A KKN + L K + +GLT L AA I+K +L R VE E+ + + + D + + L+ + H K ++ A + L IAA +GH + +D ++ K + + A ++ E + ER + K T LH A+ N+ D+VK ++ + + N T L +A + + VL Q + N ++ TPLH A G D LI++ N++ GLT LH +G + + +V GA+ + + I + +KF L + + R G + LH A + ++ LL GA + + SNG SPL
Sbjct: 210 IAAHYENMSVAQLL------LNRGANVNFTPK----NGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAK-DDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFT-PLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGY--TPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKM-------------------VKF-------LLQQQAHVNAKTRMG--YTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPL 769 HSP 2 Score: 74.3294 bits (181), Expect = 5.722e-13 Identity = 145/632 (22.94%), Postives = 246/632 (38.92%), Query Frame = 2
Query: 113 EELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIEL---FHTENLI-----------------AAIKKNDP-------IEFEKL--CKSYDFMS--QKL-----------HTGLTILQTAADSNADKIIKCILDRPVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSI------WVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENE---ITIPFEKSIIALSLMENALT---LSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALL 1843
E L + LE K KG+ +HIA G +V L+N N+ + G+ P++ A ++E+VK LL++ T+ G TP +A + + + L + T+ + A + +NDP F L Y+ MS Q L G+T L A+ +++ +LDR +I+ K E+T + N I I+ +H A I A NG S + +AA+ H +D + + YN I + + A + + + ++ +R FT LH A N + +L H + L++ E+ T L +A + H +K L Q + N N +TPLH A +G + +L+ + +D T LHCA G + + + ++++ AN + E I I + + + + T ++ K+ GK+ + L NA N + LH+AV ++ K L+ G NG +PL K N+ IE+ ++L+ GA N +S IT AS+ E M+A+L
Sbjct: 61 ELLHSGIELEATTK----KGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTED--GFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDR---GAQIDAQTKDELTPLHCAARNGHVRII---EILLEHGAP----IQAKTKNG------LSPIHMAAQGDH---MDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH--SASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKY-GKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLH----------IAAKQNQ-IEVASVLLQNGASPNCESLQGITPLHLASQ-EGRPDMVAML 652 HSP 3 Score: 70.4774 bits (171), Expect = 9.852e-12 Identity = 123/568 (21.65%), Postives = 226/568 (39.79%), Query Frame = 2
Query: 155 LIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIEL---------FHTENLIA----AIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDR--PVENKEINDLMK-EITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIP---FEKSIIALSLMENALT-LSIKFHG---KLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRIT 1789
L + G P+ +A ++G N+V LL+N + +H A N + +LLQ+ D +K G TP IA + +L F +N I A ++ + + L + + LT L AA + +II+ +L+ P++ K N L + + D + + ++ + Y + I ++ A G H++A + L+ A + + ++G I K +R++ + + + E+ + T LH A ++VK +L +++N + T L +A H + + L Q + + K QTPLH A G + L++ N D G T LH A +G + +++ GA +K + F S ++ + E+ + +N LT L + H + + K S S AR+G + LH+A K Q E+ LL +GA + + G +PL + R ++ +LI A++N+ + N +T
Sbjct: 137 LPTEDGFTPLAVALQQGHENVVALLINYGTKGKVR----LPALHIAARNDDTRTAAVLLQNDPNPDVLSK--TGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHM-DCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMA-KVLLDKGA-KANARALNGFTPLHIACKKNHMRSM------------DLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQ--RGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNG--YTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLH--LASQEGRP---------DMVAMLISKQANVNLGNKNGLT 668 HSP 4 Score: 57.7658 bits (138), Expect = 6.781e-8 Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 2
Query: 170 GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
G+ P+H+AC G + +V LL + ++ + GY P+H A G+ +IV LLL+H + T G +P IAK+ I++
Sbjct: 699 GYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSN--GTSPLGIAKRLGYISVIDVL 784
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837]) HSP 1 Score: 75.485 bits (184), Expect = 2.512e-13 Identity = 137/662 (20.69%), Postives = 256/662 (38.67%), Query Frame = 2
Query: 167 KGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIEL---FHTENLIAAIKKND-----------PI----EFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILD--RPVENKEIN--------------DLMKEITEENDKLSNLTKECILYRSII----HQHAA------GFKKFINAAVING--PHKIATQ-------------------------SFLEIAAEQGHELFIDGIMKKTIYNKSIWVR---AIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKD----FQMAFDNHWFQY 1918
KG+ +HIA G +V L+N N+ + G+ P++ A ++E+VK LL++ T+ G TP +A + + + L + T+ + D P+ +E L + +++ + LT L AA + +II+ +LD P++ K N D +K++ + N ++ ++T + + + H A G K N+ +NG P IA + + L +A+ GH + +++K + V+ + A E+ E + + +++ K + T LH A ++VK +L+H N +S + L +A H + +++L E K TPLH A G D L+ R N + G GLT LH AV +V +V+ G + + + +N++ +A SL++ HG ++NA + LH+A + +P++ L+ A V++ N +G +PL + + + I +IL+ GA + + T A N +++ LL N +SK Q + +H+ +Y
Sbjct: 72 KGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTED--GFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKP--NSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANP--NSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVE-------VANSLLQ---------HG------------ASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGH-----------VGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQ---ANVNSKTRVEYIQCHYKDHYCEY 685 HSP 2 Score: 51.2174 bits (121), Expect = 6.469e-6 Identity = 25/92 (27.17%), Postives = 47/92 (51.09%), Query Frame = 2
Query: 158 IDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF 433
+ + G P+H+A G LNIV +LL + + ++ P+H A+ +G+ E+ + LLQ+ +D + K TP A + + ++L
Sbjct: 338 VTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKD--DQTPLHCAARMGHKELVKLL 427
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Match: ENSORLT00000038944.1 (tankyrase-1 [Source:NCBI gene;Acc:101158122]) HSP 1 Score: 72.4034 bits (176), Expect = 2.289e-12 Identity = 122/579 (21.07%), Postives = 221/579 (38.17%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCR---IDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADK---IIKCILDRPVENKEIN-DLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKF-VELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSN--NPIFKN 1867
D G P+H AC G + LLL + D+W + P+H A +E+ LLL H ++C +K + P K+ + F +L+ A ++ D + +K + HT T L A S K + + +L + E N D M + ++ N E ++ +H A +NA G Q+ L AA GH L ++ + S+ A + +Q+ + E + + S V++ +L +A D D +K + P N++ T L A + ++ L + + ++K PLH A + G + L+ +++ T LH A KG +C+ ++ +GA+ K + N + + +I L + ++ LC D +N + R N++ LH+A E+ + LL+ GA V+ + G PL + ++I +LI Y +N T A++ ++ LL++ +P KN
Sbjct: 346 DKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHSKSSVDMAPTPELKERLTYE----FKGHSLLQAAREADMAKAKKTLALEIINFKHPHTHETALHCAVASPHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDIME------VLQKHGA----KVNALDTLG------QTALHRAALAGH-LQTCRLLLGYGADASLVSLQGFTAAQMGNEAVQQILNESVPVRNS--DVDYRLLEAAKA-GDLDTIKSLCT-PQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVQ-----KLCSPDNINCRDTQGR---NSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGH-----------VDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKN 880 HSP 2 Score: 56.6102 bits (135), Expect = 1.586e-7 Identity = 40/152 (26.32%), Postives = 65/152 (42.76%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKL-------CKSYDFMSQKLHTGLTILQTAADSNADKIIKCIL 595
DD G P+H AC G +V LLL + D W Y P+H A + G I++ +LLQH D + G + D+A+ ++ + + L+ A + + + L C + D T L AA N +I++ +L
Sbjct: 193 DDGGLIPLHNACSFGHSEVVSLLLCQGAEPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGA--DPNIRNTDGKSALDLAEPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKS------TPLHLAAGYNRVRIVQLLL 336 HSP 3 Score: 52.7582 bits (125), Expect = 2.348e-6 Identity = 27/79 (34.18%), Postives = 37/79 (46.84%), Query Frame = 2
Query: 161 DDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIA 397
D G P+H A G ++I LL+ N + D W + P+H A G ++ LLL H D K G TP D+A
Sbjct: 814 DKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGA--DPTMKNQEGQTPLDLA 890
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Match: SMESG000003596.1 (SMESG000003596.1) HSP 1 Score: 1763.81 bits (4567), Expect = 0.000e+0 Identity = 887/892 (99.44%), Postives = 887/892 (99.44%), Query Frame = 2
Query: 65 MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNLKKKEYVNDKICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS 2740
MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIKKAKDKKFKEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE FTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNLIKLLFSKSSSSFQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNLKKKEYVNDKICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS
Sbjct: 1 MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIKKAKDKKFKEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVE-----FTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTEPNLIKLLFSKSSSSFQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNLKKKEYVNDKICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS 887
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Match: SMESG000001281.1 (SMESG000001281.1) HSP 1 Score: 999.579 bits (2583), Expect = 0.000e+0 Identity = 519/895 (57.99%), Postives = 665/895 (74.30%), Query Frame = 2
Query: 65 MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELFHTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAF-VQCKTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNLKKKEYVNDK---ICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYF 2737
M F F +LM+G + LR +R+ FVNK++D+ G +PIH+AC G+++IV LLL+ NCNIE E+ WG KPIH A+ NGN EIVKLLL+ KC+ID +K G +P D A ++TDCIEL E L AAI +N+ ++F++ K DF Q + TGL+IL+TA NA +IIKCILD+P KEINDL++E+T + DK LT+ECILY +IHQHA GFKKF++ +ING K+ SFLEIA +G FIDGIMKKTIYNK +W AI +A++K F EI+EE++ERM L ESR KVNFTILH+AICDND +MVKYI++HPN +DL+SNNET GTAL+L HSLHHRKCIKVLQQY+L TNT+NKYSQTPLHEAIASG +NC YLI ++I+ID GF+GLT LHCAVI+GME VC Q++N GA+Y++F+EL+ +SS NEI IPF +SII L+ ENALT S+KFH LF CFL++L+S +SN DG+ NSLLH+AVKCL+P +CKSLL +GA VD VN GNSPL EMFC S++T + +N+MI ITNILI +GA+IN S+N IT+FQ AS +E+E +MLALLSNNP FSS DFQ AF WF YAE L+ +L ++FL SNIT F + K + LIKLLF KSS Q+AIIE LL +A+ +D LF+ F+K EI PK+ GENLFT ILK K+ FAWK+L S N++KFVNLKN NQ PLQ+AIE++EMDLVD LIHES+E DCED G +VLT+L+ KIS N K ++++ +KSAILLG DIYK++S G+RIID+CT+ + K+EIERFH NN+KK + ND +C IC +N V++ FQCGHL CSVC +R+ CP+C +I +TK+Y
Sbjct: 1 MRDFKNFKEYLMSGNSAGLRNNMRVGFVNKIVDEDGQYPIHVACAAGYIDIVKLLLDYNCNIEFENEWGMKPIHSASKNGNTEIVKLLLKRKCQID--SKDILGNSPLDWATMCNKTDCIELLRIEELFAAINQNNLMKFDEFYKPCDFAKQNISTGLSILETAIKHNAYQIIKCILDKPANYKEINDLIEELTMKGDKNLKLTEECILYLCVIHQHAQGFKKFVSPDIINGQCKLVGLSFLEIATGKGCNAFIDGIMKKTIYNKLLWQNAILEAQEKGFVEIEEELSERMILLESRQKVNFTILHTAICDNDAEMVKYIVNHPNAVDLNSNNETFGTALSLGHSLHHRKCIKVLQQYKLNTNTRNKYSQTPLHEAIASGSGENCEYLIEQAIDIDWAGFIGLTALHCAVIRGMETVCLQLLNKGADYVRFIELSH-----RSSSNEIAIPFIRSIIPLTNSENALTFSMKFHRTLFSCFLNRLDSQSSNVHDGDKNSLLHLAVKCLRPGICKSLLKAGANVDSVNYWGNSPLLEMFCKPQSKLTNKSLNKMIIITNILIKHGANINAISSNGITVFQFASIIESENLMLALLSNNPDLNFFSSSDFQTAFTKQWFNYAEILINILPETFLVSNITLATFDLSTKADTKLIKLLFDKSSCEMQQAIIEKLLCDAVNSDDIDLFEHFLKTEILVPKILKTGENLFTFILKENKVEFAWKLLNSINQVKFVNLKNSNNQIPLQLAIELEEMDLVDTLIHESDELDCEDAAGKTVLTRLMLKISNLP-ANHKPKMISVIKSAILLGADIYKMTSNGQRIIDSCTESHVKAEIERFHKNNVKKSKIKNDNERGMCNICTDNLVNIVFQCGHLCCSVCADRVRFCPLCKAKIITRTKMYL 887
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Match: SMESG000002106.1 (SMESG000002106.1) HSP 1 Score: 974.926 bits (2519), Expect = 0.000e+0 Identity = 500/903 (55.37%), Postives = 671/903 (74.31%), Query Frame = 2
Query: 65 MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF-HTENLI---AAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQC-KTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNL---KKKEYVNDK---ICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS 2740
M + F+ LMNG TE + + EFVN +I +KG FPIH+ACEKG +NIV LL+ CNIECED +PIHYA++NG+++I+K+LL +KC++DC+ K GL+P ++A+ + DC+EL ++E L+ AA+K+NDPIEFE+L KS +F+S+ +TG L+ A ++NAD+IIK ILD+P++NKEI+D MKEI+ N + SNL KEC+L+R IIH+H GFK+ AA+ING +I SFLE+AAEQG +FID IMKKTIY+KS+W++A+ KA++ F I+EEI++RM L ESR KVNFTILH+AICDND D+VKYILDH + +DL+SNNE GTALA +HSLHH KCIK++QQY+L TNT+NK+ QTPLHEAIASG++ NCG+LI+R++NIDQ F+GL+ LHCAVI+GM NV ++V GA+Y+K+V Q T N+ITIPF KS I+LS ENALTLSIKF L CFL++LNS ++N+ DG+ NSLLHMAVKCLQP LC+SL+++GA++ +N+ GN+PL EMF + MT+EK+N+MI+ITNILI GAD+N KS+N I++FQSAS + +ER+MLAL+SNNP+ +NFSS DFQ+AF N W +Y E L+K+L D+FL SNIT E F K EP LIK+LF KSS Q++I+E L+ A + ++FD I IEI P NGE L T ++K KI F WK+L+S NK KFVN+KNK NQT LQVAIEI+EMDL+D LI+ES FD ED +G + LTQL+ KISIC N NS +L++A+KS + GGDIY V+S GK++ID C D N K+++ F+ L KK++ +DK IC ICM+N V++ QCGHL+C C ++LFVC CN EI + ++YF+
Sbjct: 1 MRDYKCFMESLMNGNTEGVNNNINEEFVN-IIVEKGKFPIHVACEKGLINIVQLLMQHGCNIECEDDKQCRPIHYASMNGHVKIIKVLLLNKCQVDCKQKN--GLSPLELARLCDQKDCVELLSNSETLLKTFAAVKRNDPIEFEELYKSCNFISRNFYTGFCFLKIALENNADRIIKNILDKPIDNKEIDDFMKEISANNIENSNLIKECLLHRCIIHKHVVGFKQLKTAAIINGNREIMQTSFLEMAAEQGCHVFIDEIMKKTIYDKSVWIKAMDKARENGFLNIEEEISDRMILLESRQKVNFTILHTAICDNDPDVVKYILDHADIVDLNSNNEIFGTALAFSHSLHHSKCIKIMQQYQLDTNTRNKFMQTPLHEAIASGVEKNCGFLIDRAVNIDQTDFMGLSVLHCAVIRGMINVSSRLVTMGADYVKYV-----QLTPHLLANKITIPFAKSTISLSHTENALTLSIKFQATLLPCFLERLNSESANSCDGDENSLLHMAVKCLQPGLCRSLVNAGAQIHSINNLGNTPLLEMFYETEINMTDEKLNKMIDITNILIECGADVNAKSSNEISLFQSASIIGSERLMLALVSNNPMLRNFSSDDFQLAFSNQWHKYGETLIKILPDNFLSSNITIETFQSSKKAEPKLIKILFDKSSPELQQSILENFLYEASNNDGNEIFDHMINIEILQPSELRNGETLLTALMKENKIGFVWKLLDSINKTKFVNVKNKRNQTALQVAIEIEEMDLIDNLIYESEGFDSEDKDGRTALTQLLEKISICTNANSNRKLISAIKSIVQAGGDIYMVTSLGKQVIDACADGNVKADVIEFNRKILTKSKKEKNDDDKGSMICNICMDNLVEIVLQCGHLLCCRCSDQLFVCHACNAEIKTRIQLYFN 895
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Match: SMESG000002106.1 (SMESG000002106.1) HSP 1 Score: 974.156 bits (2517), Expect = 0.000e+0 Identity = 499/900 (55.44%), Postives = 670/900 (74.44%), Query Frame = 2
Query: 74 FDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLLQHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF-HTENLI---AAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQC-KTEPNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGKRIIDTCTDCNAKSEIERFHGNNL---KKKEYVNDK---ICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS 2740
+ F+ LMNG TE + + EFVN +I +KG FPIH+ACEKG +NIV LL+ CNIECED +PIHYA++NG+++I+K+LL +KC++DC+ K GL+P ++A+ + DC+EL ++E L+ AA+K+NDPIEFE+L KS +F+S+ +TG L+ A ++NAD+IIK ILD+P++NKEI+D MKEI+ N + SNL KEC+L+R IIH+H GFK+ AA+ING +I SFLE+AAEQG +FID IMKKTIY+KS+W++A+ KA++ F I+EEI++RM L ESR KVNFTILH+AICDND D+VKYILDH + +DL+SNNE GTALA +HSLHH KCIK++QQY+L TNT+NK+ QTPLHEAIASG++ NCG+LI+R++NIDQ F+GL+ LHCAVI+GM NV ++V GA+Y+K+V Q T N+ITIPF KS I+LS ENALTLSIKF L CFL++LNS ++N+ DG+ NSLLHMAVKCLQP LC+SL+++GA++ +N+ GN+PL EMF + MT+EK+N+MI+ITNILI GAD+N KS+N I++FQSAS + +ER+MLAL+SNNP+ +NFSS DFQ+AF N W +Y E L+K+L D+FL SNIT E F K EP LIK+LF KSS Q++I+E L+ A + ++FD I IEI P NGE L T ++K KI F WK+L+S NK KFVN+KNK NQT LQVAIEI+EMDL+D LI+ES FD ED +G + LTQL+ KISIC N NS +L++A+KS + GGDIY V+S GK++ID C D N K+++ F+ L KK++ +DK IC ICM+N V++ QCGHL+C C ++LFVC CN EI + ++YF+
Sbjct: 5 YKCFMESLMNGNTEGVNNNINEEFVN-IIVEKGKFPIHVACEKGLINIVQLLMQHGCNIECEDDKQCRPIHYASMNGHVKIIKVLLLNKCQVDCKQKN--GLSPLELARLCDQKDCVELLSNSETLLKTFAAVKRNDPIEFEELYKSCNFISRNFYTGFCFLKIALENNADRIIKNILDKPIDNKEIDDFMKEISANNIENSNLIKECLLHRCIIHKHVVGFKQLKTAAIINGNREIMQTSFLEMAAEQGCHVFIDEIMKKTIYDKSVWIKAMDKARENGFLNIEEEISDRMILLESRQKVNFTILHTAICDNDPDVVKYILDHADIVDLNSNNEIFGTALAFSHSLHHSKCIKIMQQYQLDTNTRNKFMQTPLHEAIASGVEKNCGFLIDRAVNIDQTDFMGLSVLHCAVIRGMINVSSRLVTMGADYVKYV-----QLTPHLLANKITIPFAKSTISLSHTENALTLSIKFQATLLPCFLERLNSESANSCDGDENSLLHMAVKCLQPGLCRSLVNAGAQIHSINNLGNTPLLEMFYETEINMTDEKLNKMIDITNILIECGADVNAKSSNEISLFQSASIIGSERLMLALVSNNPMLRNFSSDDFQLAFSNQWHKYGETLIKILPDNFLSSNITIETFQSSKKAEPKLIKILFDKSSPELQQSILENFLYEASNNDGNEIFDHMINIEILQPSELRNGETLLTALMKENKIGFVWKLLDSINKTKFVNVKNKRNQTALQVAIEIEEMDLIDNLIYESEGFDSEDKDGRTALTQLLEKISICTNANSNRKLISAIKSIVQAGGDIYMVTSLGKQVIDACADGNVKADVIEFNRKILTKSKKEKNDDDKGSMICNICMDNLVEIVLQCGHLLCCRCSDQLFVCHACNAEIKTRIQLYFN 896
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Match: SMESG000010368.1 (SMESG000010368.1) HSP 1 Score: 959.133 bits (2478), Expect = 0.000e+0 Identity = 505/934 (54.07%), Postives = 663/934 (70.99%), Query Frame = 2
Query: 65 MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDK-GHFPIHIACEKGFLNIVILLLNENCNIECEDIWGYKPIHYATLNGNIEIVKLLL---------------------------------QHKCRIDCRTKGWFGLTPFDIAKKWSRTDCIELF-HTENLIAAIKKNDPIEFEKLCKSYDFMSQKLHTGLTILQTAADSNADKIIKCILDRPVENKEINDLMKEITEENDKLSNLTKECILYRSIIHQHAAGFKKFINAAVINGPHKIATQSFLEIAAEQGHELFIDGIMKKTIYNKSIWVRAIXXXXXXXXXEIQEEITERMNLFESRIKVNFTILHSAICDNDTDMVKYILDHPNNIDLDSNNETLGTALALAHSLHHRKCIKVLQQYELATNTQNKYSQTPLHEAIASGLDDNCGYLINRSINIDQIGFLGLTGLHCAVIKGMENVCEQMVNNGANYMKFVELNFLQFTCQSSENEITIPFEKSIIALSLMENALTLSIKFHGKLFLCFLDKLNSTTSNARDGNNNSLLHMAVKCLQPELCKSLLDSGAKVDVVNSNGNSPLFEMFCNSNSRMTEEKVNRMIEITNILIGYGADINVKSTNRITIFQSASKLENERIMLALLSNNPIFKNFSSKDFQMAFDNHWFQYAECLLKLLSDSFLESNITNEAFVQCKTE-PNLIXXXXXXXXXXXQRAIIETLLWNAIRKNDKKLFDFFIKIEISSPKLFSNGENLFTQILKVRKIHFAWKVLESKNKMKFVNLKNKTNQTPLQVAIEIDEMDLVDALIHESNEFDCEDINGNSVLTQLIGKISICKNVNSKSRLMAAMKSAILLGGDIYKVSSCGK-RIIDTCTDCNAKSEIERFHGNNLKKKEYVNDK-----ICIICMNNTVDVAFQCGHLVCSVCGNRLFVCPVCNKEISNKTKIYFS 2740
M F F HLM G E +R LR EFV+K+IDD G FPIHI C GF+ + LLL+ NC IECE WG +PIHYAT G+ EIVKLLL + KCRIDC+TK G +P DIAK+++R DC++L H E L A I+++DPIE EKL S +F+ + ++TGL +L+ A +NADKI+K ILD+ ++NKEI++++KE+T + + +NL KECILY I H +G K+F +A VING +I ++LE+A E G +L ID IM+KTIY+KSIW++AI KAK+ F EIQ+EI ERM L ESR K NFTI H+AI DND DM++Y+LDHPN +DL+SNNET GTAL+LA+SLH R CI VLQQY+L NT+NKYS+TPLHEAIASG+D +CGYLI+RSI+IDQ G++GLTGLHCAVI+G+ NVC Q+VN GA+ M+ V L +CQ S EI+IPF +S I+L+++EN+LTLS+KFH +F CF KL+ +SNA DG+NNSLLH+AVKCLQP++C SLL++GA+VD VN+ GNSPL E+F + + ++ +N++I ITNILI GAD+N KS+N I+ F SAS +E+E++M+AL+SN+P NFSS DFQ AF N WF YAE L++LL D FL I+N+ F C++ NLIK LF+KSSS+ Q+++IE LL+ A+ ND +LFD FI+ EIS+PK NGENL T IL+ RKI FAWK+L+S N+ KFV L+N NQ PLQ+AIEI+EMDLV+ALI E+ + ED G L QL+ KISI NVN K +L+ A+KS I GGDIYK++S G+ RIID CTD AK EIER+H NN K N +C IC N V++ FQCGHL+C +C ++LF CP C +I +TK++FS
Sbjct: 1 MGDFQSFKEHLMKGNAEGVRNNLRGEFVDKIIDDDNGDFPIHITCRDGFVAVAKLLLDRNCKIECETKWGNRPIHYATCYGHSEIVKLLLDRNCNIECETKWKWRPIHYVSWKGQSEILKLLLRRKCRIDCKTKD--GKSPLDIAKQFNRVDCVKLLTHMEKLFAIIEQDDPIEMEKLLISCEFIRENIYTGLNVLEIAMTNNADKILKWILDKSIDNKEIDEVIKELTAIDKENANLLKECILYLCITRHHMSGIKRFHSAVVINGQRRIVGPTYLELAVEHGCDLLIDLIMRKTIYDKSIWIKAIGKAKENGFIEIQKEIQERMTLMESRQKSNFTIFHTAISDNDVDMLQYLLDHPNIVDLNSNNETFGTALSLAYSLHQRNCIAVLQQYQLDVNTRNKYSETPLHEAIASGMDADCGYLIDRSIDIDQTGYMGLTGLHCAVIRGLINVCTQLVNRGADCMRSVNL-----SCQHSVGEISIPFAQSFISLTVIENSLTLSLKFHRTIFPCFFKKLDLNSSNAHDGDNNSLLHLAVKCLQPDICSSLLNAGAQVDSVNNFGNSPLLELFADRKIPVDDDTLNKLIGITNILIVKGADVNFKSSNGISAFISASNIESEKLMVALVSNHPRLINFSSNDFQTAFLNQWFVYAELLIELLPDDFLSFAISNDTFQSCESAGTNLIKSLFNKSSSALQQSVIENLLYKAVNSNDVELFDHFIRTEISAPKSLRNGENLLTFILQERKIEFAWKLLDSVNRGKFVKLRNINNQIPLQIAIEIEEMDLVNALIIETKDCHSEDEGGTCALIQLLEKISISNNVNFKRKLILAIKSIISFGGDIYKMTSRGQHRIIDVCTDSVAKEEIERYHRNNTKPSTEENKPDNEIAMCNICQNKQVNIVFQCGHLICDLCSDQLFECPFCKADIVTRTKMFFS 927 The following BLAST results are available for this feature:
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of RING domain ligase2 isoform 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026726 ID=SMED30026726|Name=RING domain ligase2 isoform 3|organism=Schmidtea mediterranea sexual|type=transcript|length=2953bpback to top protein sequence of SMED30026726-orf-1 >SMED30026726-orf-1 ID=SMED30026726-orf-1|Name=SMED30026726-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=893bp MSYFDMFVSHLMNGKTEELRRTLRLEFVNKLIDDKGHFPIHIACEKGFLNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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