SMED30026702
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026702 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30026702 aligns in the following genomic locations:
Homology
BLAST of SMED30026702 vs. Planmine SMEST
Match: SMESG000038479.1 (SMESG000038479.1) HSP 1 Score: 1126.31 bits (2912), Expect = 0.000e+0 Identity = 593/608 (97.53%), Postives = 600/608 (98.68%), Query Frame = 3 Query: 27 MSESLTFDGFVILDDDVTHXXXXXXXXXXXXXXGKTGVTNIKNTPNLTNVTNDLFYKVDNLKGHDSNTIIDVVECDSTTPTSLEKVXXXXXXXXXXXXXXAFPSYPVTLIDSYMCNVNESTNEIENTMNNVVKAAESVPCDNFNIVLLTNALNEHDELHRNDIPLEEISEKDSMTTMENHGSSSIVKSSNEMNTNLDVGIDSGEDRTDFRINILDRNDEENKWSTNQYIQQDNEENKCTMKEEISESLKEVKNQENVESIDMVNKLSSEGEENDVGIRTEKEDNWSTNQYLQDDKEETQLSQKDENIKANNEDKPDFRVNIFERNDRVDTWLTNQHIYEETEDRKSISNIENEFNPISTIDDEFECDANKTLVDAKLCISDRLTDLAEYVSDCRENPLVESDSKSGNKEDIETTENVSNLMSNQLDNDQQSPNAKENIITDGNERQSAMDVDNRKITQETPDTVPLESSDVAVSVTGGSITAVTAPPLIGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDISPEDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGFKYFLKVINTVTKSVVSENENF 1850 MSESLTFDGFVILDDDVTHSILDSNSDDLYYSSGKTGVTNIKNTPNLTNVTNDLFYKVDNLKGHDSNTIIDVVECDSTTPTSLEKVELDNECNENENDNLAFPSYPVTLIDSYMCNVNESTNEIENTMNNVVKA ESVPCDNFNIVLLTNALNEHDELHRNDIPLEEISEKDSMTTMENHGSSSIVKSSNEMNTNLDVGIDSGEDRTDFRINILDRNDEENKWSTNQYIQQDNEENKCT+KEEISESLKEVK+QENVESID+VNKLSSE EEND+GIRTEKEDNWSTNQYLQDDKEETQLSQKDENIKANNEDKPDFRVNI ERNDRVDTWLTNQHIYEETEDRKSISNIENEFNPIST+DDEFECDANK LVDAKLCISDRLTDLAEYVSDCRENPLVESDSKSGNKEDIETTENVSNLMSNQLDNDQQSPNAKENIITDGNERQSA+ VDNRKITQETPDTVPLESSDVAVSVTGGSITAVTAPPLIGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDIS EDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGF YFLKVINTVTKSVVSENE + Sbjct: 1 MSESLTFDGFVILDDDVTHSILDSNSDDLYYSSGKTGVTNIKNTPNLTNVTNDLFYKVDNLKGHDSNTIIDVVECDSTTPTSLEKVELDNECNENENDNLAFPSYPVTLIDSYMCNVNESTNEIENTMNNVVKATESVPCDNFNIVLLTNALNEHDELHRNDIPLEEISEKDSMTTMENHGSSSIVKSSNEMNTNLDVGIDSGEDRTDFRINILDRNDEENKWSTNQYIQQDNEENKCTIKEEISESLKEVKDQENVESIDIVNKLSSEREENDMGIRTEKEDNWSTNQYLQDDKEETQLSQKDENIKANNEDKPDFRVNILERNDRVDTWLTNQHIYEETEDRKSISNIENEFNPISTVDDEFECDANKILVDAKLCISDRLTDLAEYVSDCRENPLVESDSKSGNKEDIETTENVSNLMSNQLDNDQQSPNAKENIITDGNERQSAIYVDNRKITQETPDTVPLESSDVAVSVTGGSITAVTAPPLIGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDISLEDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGFNYFLKVINTVTKSVVSENEKY 608
BLAST of SMED30026702 vs. Planmine SMEST
Match: SMESG000038478.1 (SMESG000038478.1) HSP 1 Score: 136.346 bits (342), Expect = 5.495e-38 Identity = 66/71 (92.96%), Postives = 69/71 (97.18%), Query Frame = 3 Query: 2226 GVFLNSKQGAVLYSLRSVIDEILMNNTAFNSVKCLSDFWYMIESKSVKQIGELELINEYNVGFMFKKLQDY 2438 G+ + +KQGAVLYSLRSVIDEILMNNTAFNSVKCLSDFWYMIESKSVKQIGELELINEYNVGFMFKKLQDY Sbjct: 2 GICIIAKQGAVLYSLRSVIDEILMNNTAFNSVKCLSDFWYMIESKSVKQIGELELINEYNVGFMFKKLQDY 72
BLAST of SMED30026702 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1) HSP 1 Score: 96.2857 bits (238), Expect = 6.021e-20 Identity = 72/234 (30.77%), Postives = 122/234 (52.14%), Query Frame = 3 Query: 1491 IGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDISPEDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGFKYFLKVINTVTK-SVVSENENFKDTD-DIMFDKFTSKNKGFVTDSFCGQIKHKSFCKNCQKIYSEEYEQTYXXXXXXXXXXXXXXXXXCIQSYLSDVKIEITKDHKC-CNNPSI--WANKEFTHTPDYLILCIDR 2177 IG NP F + VI L++CTE + LI YH D I +S+ S +D F+NN KL E+ ND KI E+ I+ YF + D L F L +++ VTK +++ N + + + ++ F+ F ++N GFV SF GQ+ + C +C+ + Y+ S+ L +P + ++ L++ I +L + +E+ C C + S+ + E+TH P YL + ++R Sbjct: 895 IGFENPKGFCFLNCVIQQLYSCTEFKDDLIQYHRGDSIAT--DQSNPQASDDDKIFVNNFSKLMEEINNRNDRKIRELVSEIQKYFIESSSGDDVLKCFMTVLTILSNVTKVKILNRNNSIQYINSEVSFENFKNQNNGFVFRSFYGQMALRLVCVHCKYSVNTSYQLFKSVALPVPETRSTIRLDEIINGFLQESPVEMDDKRNCKCPDGSLNFLSITEYTHMPRYLHIYLNR 1126
BLAST of SMED30026702 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1) HSP 1 Score: 96.2857 bits (238), Expect = 6.640e-20 Identity = 80/293 (27.30%), Postives = 139/293 (47.44%), Query Frame = 3 Query: 1383 NRKITQETPDTVPLESSDVAVSVT------------GGSITAVTAPPL-----------IGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDISPEDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGFKYFLKVINTVTK-SVVSENENFKDTD-DIMFDKFTSKNKGFVTDSFCGQIKHKSFCKNCQKIYSEEYEQTYXXXXXXXXXXXXXXXXXCIQSYLSDVKIEITKDHKC-CNNPSI--WANKEFTHTPDYLILCIDR 2177 N++ +E + V +ES+ + S++ G P IG NP F + VI L++CTE + LI YH D I +S+ S +D F+NN KL E+ ND KI E+ I+ YF + D L F L +++ VTK +++ N + + + ++ F+ F ++N GFV SF GQ+ + C +C+ + Y+ S+ L +P + ++ L++ I +L + +E+ C C + S+ + E+TH P YL + ++R Sbjct: 845 NKRFMKENDNVVRIESNSIGRSMSNLEVKRRDRRSFGSESNMSNVTPYNYQNNNILRDPIGFENPKGFCFLNCVIQQLYSCTEFKDDLIQYHRGDSIAT--DQSNPQASDDDKIFVNNFSKLMEEINNRNDRKIRELVSEIQKYFIESSSGDDVLKCFMTVLTILSNVTKVKILNRNNSIQYINSEVSFENFKNQNNGFVFRSFYGQMALRLVCVHCKYSVNTSYQLFKSVALPVPETRSTIRLDEIINGFLQESPVEMDDKRNCKCPDGSLNFLSITEYTHMPRYLHIYLNR 1135
BLAST of SMED30026702 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1) HSP 1 Score: 88.5817 bits (218), Expect = 1.562e-17 Identity = 70/234 (29.91%), Postives = 119/234 (50.85%), Query Frame = 3 Query: 1491 IGVSNPINFPAMIAVISVLFNCTELRCGLIDYHHSDEIVNFDIESSTDISPEDVFFMNNLCKLFVEMKASNDEKIIEIAGLIRNYFKKKKIQLDFLNGFKYFLKVINTVTK-SVVSENENFKDTD-DIMFDKFTSKNKGFVTDSFCGQIKHKSFCKNCQKIYSEEYEQTYXXXXXXXXXXXXXXXXXCIQSYLSDVKIEITKDHKC-CNNPSI--WANKEFTHTPDYLILCIDR 2177 IG NP F L++CTE + LI YH D I +S+ S +D F+NN KL E+ ND KI E+ I+ YF + D L F L +++ VTK +++ N + + + ++ F+ F ++N GFV SF GQ+ + C +C+ + Y+ S+ L +P + ++ L++ I +L + +E+ C C + S+ + E+TH P YL + ++R Sbjct: 895 IGFENPKGFQ--------LYSCTEFKDDLIQYHRGDSIAT--DQSNPQASDDDKIFVNNFSKLMEEINNRNDRKIRELVSEIQKYFIESSSGDDVLKCFMTVLTILSNVTKVKILNRNNSIQYINSEVSFENFKNQNNGFVFRSFYGQMALRLVCVHCKYSVNTSYQLFKSVALPVPETRSTIRLDEIINGFLQESPVEMDDKRNCKCPDGSLNFLSITEYTHMPRYLHIYLNR 1118 The following BLAST results are available for this feature:
BLAST of SMED30026702 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30026702 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30026702 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30026702 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30026702 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026702 ID=SMED30026702|Name=SMED30026702|organism=Schmidtea mediterranea sexual|type=transcript|length=2764bpback to top protein sequence of SMED30026702-orf-1 >SMED30026702-orf-1 ID=SMED30026702-orf-1|Name=SMED30026702-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=805bp MSESLTFDGFVILDDDVTHSILDSNSDDLYYSSGKTGVTNIKNTPNLTNVback to top Annotated Terms
The following terms have been associated with this transcript:
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