GRIP1-associated protein 1

Overview
NameGRIP1-associated protein 1
Smed IDSMED30026701
Length (bp)2032
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GRIP1-associated protein 1 (SMED30026701) t-SNE clustered cells

Violin plots show distribution of expression levels for GRIP1-associated protein 1 (SMED30026701) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GRIP1-associated protein 1 (SMED30026701) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GRIP1-associated protein 1 (SMED30026701) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026701

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30026701SMESG000055144.1 dd_Smed_v4_8789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Human
Match: GRIPAP1 (GRIP1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:18706])

HSP 1 Score: 54.299 bits (129), Expect = 6.689e-7
Identity = 33/108 (30.56%), Postives = 64/108 (59.26%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
             +++  EFQR Q QL+ L+   Y++ ++       L+    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    3 QALSEEEFQRMQAQLLELRTNNYQLSDELRKNGVELTSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQMEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Human
Match: GRIPAP1 (GRIP1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:18706])

HSP 1 Score: 54.299 bits (129), Expect = 6.719e-7
Identity = 33/108 (30.56%), Postives = 64/108 (59.26%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
             +++  EFQR Q QL+ L+   Y++ ++       L+    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    3 QALSEEEFQRMQAQLLELRTNNYQLSDELRKNGVELTSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQMEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Zebrafish
Match: gripap1 (GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070112-922])

HSP 1 Score: 58.9214 bits (141), Expect = 1.988e-8
Identity = 36/111 (32.43%), Postives = 65/111 (58.56%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQ 469
             +++  EF R Q QL+ L+   Y++ ++       L+    K  +LE++ +KA    NK K A+++D L+NEN++L+ +L + E +F+LQN  +  E   L S+ E L+S+
Sbjct:    3 QALSEEEFHRMQAQLLELRTQNYQLSDELRKNTAELNSVRQKISNLEKDYVKAQKALNKSKKAQEVDALINENKMLQGKLHSQEDDFRLQNSTLMQELSKLCSQIELLESE 113
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Mouse
Match: Gripap1 (GRIP1 associated protein 1 [Source:MGI Symbol;Acc:MGI:1859616])

HSP 1 Score: 55.0694 bits (131), Expect = 2.606e-7
Identity = 34/107 (31.78%), Postives = 64/107 (59.81%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            +++  EFQR Q QL+ L+   Y++ ++       LS    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    4 ALSEEEFQRMQTQLLELRTNNYQLSDELRKNGVELSSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQLEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Mouse
Match: Gripap1 (GRIP1 associated protein 1 [Source:MGI Symbol;Acc:MGI:1859616])

HSP 1 Score: 55.0694 bits (131), Expect = 2.707e-7
Identity = 34/107 (31.78%), Postives = 64/107 (59.81%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            +++  EFQR Q QL+ L+   Y++ ++       LS    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    4 ALSEEEFQRMQTQLLELRTNNYQLSDELRKNGVELSSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQLEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. UniProt/SwissProt
Match: sp|Q8VD04|GRAP1_MOUSE (GRIP1-associated protein 1 OS=Mus musculus OX=10090 GN=Gripap1 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.894e-6
Identity = 34/107 (31.78%), Postives = 64/107 (59.81%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            +++  EFQR Q QL+ L+   Y++ ++       LS    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    4 ALSEEEFQRMQTQLLELRTNNYQLSDELRKNGVELSSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQLEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. UniProt/SwissProt
Match: sp|Q4V328|GRAP1_HUMAN (GRIP1-associated protein 1 OS=Homo sapiens OX=9606 GN=GRIPAP1 PE=1 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 3.212e-6
Identity = 33/108 (30.56%), Postives = 64/108 (59.26%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
             +++  EFQR Q QL+ L+   Y++ ++       L+    K  +L++E  KA    +K K A+++++L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L
Sbjct:    3 QALSEEEFQRMQAQLLELRTNNYQLSDELRKNGVELTSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQMEQL 110
[Close]
BLAST of GRIP1-associated protein 1 vs. TrEMBL
Match: A0A3S1BU46 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_017312 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.000e-11
Identity = 48/121 (39.67%), Postives = 72/121 (59.50%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQIETYKVTKTKQ 502
            S++  EF R Q QLI L+   Y+++ K + L R+LS+S  K   LERE  KA    NK K AKD++ L+ E+  L+ +L + E  F+LQN  + TE  +L + NE LK Q+E  + +  +Q
Sbjct:    4 SLSEGEFHRMQLQLIELRTHNYELDGKNKKLERDLSESNEKVDQLEREYSKAKQALNKSKKAKDVEQLIQESDSLQRKLLSQEEEFRLQNQTLMTELASLVASNEELKKQVEASQASSAEQ 124
[Close]
BLAST of GRIP1-associated protein 1 vs. TrEMBL
Match: A0A564Z9A1 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS13527 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 4.864e-10
Identity = 48/126 (38.10%), Postives = 71/126 (56.35%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKS-------AKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLK--SQIETYKVT 490
            SI++ EF RFQ QL+ L++AKY  EE       NL+++E K + LE  L   +    + +S       A D++ L  EN  LR RL  +E +F+LQ+  +R ECE L +EN+ LK  S I +  +T
Sbjct:    4 SISAEEFARFQTQLLDLRQAKYSAEE-------NLTRAEKKAESLETALAHQSGELAELRSRLSCIQHAGDIETLTKENHCLRQRLLNVESSFQLQSSTLRAECERLQAENKLLKQYSHIPSSSLT 122
[Close]
BLAST of GRIP1-associated protein 1 vs. TrEMBL
Match: A0A0R3TZS4 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS13347 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.605e-9
Identity = 44/115 (38.26%), Postives = 70/115 (60.87%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKAN----DVKNKF---KSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLK 463
            SI++ EF RFQ+QL+ L++AKY  EE       NL++++ K + LE  L   +    +++++F   + A D++ L  EN  LR RL  +E +F+LQ+  +R ECE L +EN  LK
Sbjct:    4 SISAEEFARFQNQLLDLRQAKYFAEE-------NLTRAQKKTESLEAALAHQSGELAELRSRFSLIQHAGDIEALAKENHCLRQRLLNVESSFQLQSSTLRAECERLQAENNLLK 111
[Close]
BLAST of GRIP1-associated protein 1 vs. TrEMBL
Match: A0A2J7QMV3 (Uncharacterized protein (Fragment) OS=Cryptotermes secundus OX=105785 GN=B7P43_G07298 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.737e-9
Identity = 42/110 (38.18%), Postives = 66/110 (60.00%), Query Frame = 2 
Query:  143 ITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQI 472
            ++  EF+R Q QLI L+ A Y +EE+C     +      K   LERE  KA    +K K AKD+D+L+NEN+ L+ +L A E +F+LQN+ +  E  +L   N++L+ ++
Sbjct:    5 LSDEEFRRLQTQLIELRTANYGLEEQCTKYRSDNQALSSKLGALEREYQKAQKSIDKSKKAKDIDLLLNENENLQAKLEAQEDDFRLQNETLLRELSSLTETNDKLQHEL 114
[Close]
BLAST of GRIP1-associated protein 1 vs. TrEMBL
Match: A0A210R7C1 (GRIP1-associated protein 1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT11238 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.959e-9
Identity = 42/115 (36.52%), Postives = 68/115 (59.13%), Query Frame = 2 
Query:  140 SITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQIETYK 484
            S++  EF R Q QLI L+   Y++E KC+   R +S    K   L ++L KAN    K K AKD++IL+ EN  L+ +L + E +F+LQN+ +  E   L + NE L+ ++++ +
Sbjct:    4 SLSEEEFHRMQLQLIDLRTVNYELEGKCKKQEREISIGVEKTDQLAKDLQKANKAIQKSKKAKDVEILLQENDTLQRKLESQEDDFRLQNETLMAELTTLVAANEDLEKKVKSGR 118
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Cavefish
Match: gripap1 (GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070112-922])

HSP 1 Score: 55.0694 bits (131), Expect = 2.351e-7
Identity = 34/111 (30.63%), Postives = 63/111 (56.76%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQ 469
             +++  EF R Q QL+ L+   Y++ +        L+    K   LE++ +KA    NK K A+++D L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L+ +
Sbjct:    3 QALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELNNVRQKTLTLEKDYVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMQELSKLCSQIEQLEGE 113
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Cavefish
Match: gripap1 (GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070112-922])

HSP 1 Score: 55.0694 bits (131), Expect = 2.466e-7
Identity = 34/111 (30.63%), Postives = 63/111 (56.76%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQ 469
             +++  EF R Q QL+ L+   Y++ +        L+    K   LE++ +KA    NK K A+++D L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L+ +
Sbjct:    3 QALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELNNVRQKTLTLEKDYVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMQELSKLCSQIEQLEGE 113
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Cavefish
Match: gripap1 (GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GENE-070112-922])

HSP 1 Score: 54.6842 bits (130), Expect = 2.687e-7
Identity = 34/111 (30.63%), Postives = 63/111 (56.76%), Query Frame = 2 
Query:  137 HSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQ 469
             +++  EF R Q QL+ L+   Y++ +        L+    K   LE++ +KA    NK K A+++D L++EN++L+ +L + E +F+LQN  +  E   L S+ E+L+ +
Sbjct:    3 QALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELNNVRQKTLTLEKDYVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMQELSKLCSQIEQLEGE 113
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Medaka
Match: gripap1 (GRIP1 associated protein 1 [Source:NCBI gene;Acc:101159328])

HSP 1 Score: 58.9214 bits (141), Expect = 1.688e-8
Identity = 36/109 (33.03%), Postives = 63/109 (57.80%), Query Frame = 2 
Query:  134 NHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            + +++  EF R Q QL+ L+   Y++ +        L     K   LER+L+KA    NK K A+++D L++EN++L+ +L + E +F+LQN  + TE   L ++ E+L
Sbjct:    4 SQALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELGALRQKNGLLERDLVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMTELSKLCTQIEQL 112
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Medaka
Match: gripap1 (GRIP1 associated protein 1 [Source:NCBI gene;Acc:101159328])

HSP 1 Score: 58.5362 bits (140), Expect = 1.740e-8
Identity = 36/109 (33.03%), Postives = 63/109 (57.80%), Query Frame = 2 
Query:  134 NHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            + +++  EF R Q QL+ L+   Y++ +        L     K   LER+L+KA    NK K A+++D L++EN++L+ +L + E +F+LQN  + TE   L ++ E+L
Sbjct:    4 SQALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELGALRQKNGLLERDLVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMTELSKLCTQIEQL 112
[Close]
BLAST of GRIP1-associated protein 1 vs. Ensembl Medaka
Match: gripap1 (GRIP1 associated protein 1 [Source:NCBI gene;Acc:101159328])

HSP 1 Score: 58.5362 bits (140), Expect = 1.779e-8
Identity = 36/109 (33.03%), Postives = 63/109 (57.80%), Query Frame = 2 
Query:  134 NHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERL 460
            + +++  EF R Q QL+ L+   Y++ +        L     K   LER+L+KA    NK K A+++D L++EN++L+ +L + E +F+LQN  + TE   L ++ E+L
Sbjct:    4 SQALSEEEFHRMQAQLLELRTQNYQLSDDLRKNTAELGALRQKNGLLERDLVKAQKALNKSKKAQEVDALLSENEMLQGKLHSQEDDFRLQNSTLMTELSKLCTQIEQL 112
[Close]
BLAST of GRIP1-associated protein 1 vs. Planmine SMEST
Match: SMESG000055144.1 (SMESG000055144.1)

HSP 1 Score: 1110.52 bits (2871), Expect = 0.000e+0
Identity = 610/612 (99.67%), Postives = 612/612 (100.00%), Query Frame = 2 
Query:  128 MENHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQIETYKVTKTKQXXXXXXXXXXXXXPVHQDHEYKIRYLQHEVTKLLEEKTTIENXXXXXXXXXXXXXXXXXXXXXRINCLLNEKIKLANKCSVTEAEMNDISCNLSKQLEDQMNNYEMQLQSLKLGFAMDLEAIESKLKEEFSTKLDESTKSYENEIQRIQLEAIEQQSKLNTKIQKIIDDNNQLKTEMQLELTNTQTNYERELKEKQSEIDKILVKRQMEIEKLNSEKIELQSNLDYTQEELKLINRKNQKILKDVKKQLEKEVKLTQETQSRLEEVVXXXXXXXXXKINEYLSGVHCKMSNNDDQSSLYSSPNTGIIDNCPLSSSPLNNIAMTDEELKILLDRFTSVQNDFCESKRIIESLHFDKSQLIQTIERQQSIILNYIKNRGKNDPEIDTDSPKHNHSTSEALILNGLMSSQTSNFGLQSVMNKLKDSLKSIASKDLMSLGDKTVCRVTTAEVKQLQRVCEELMMRCLNLENDLQLAIDMNTQLKTQMNAIL 1963
            MENHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQIETYKVTKTKQDVNINNINDSVDSPVHQDHEYKIRYLQHEVTKLLEEKTTIENKNEDLLKEIKIIKESENELINRINCLLNEKIKLANKCSVTEAEMNDISCNLSKQLEDQMNNYEMQLQSLKLGFAMDLEAIESKLKEEFSTKLDESTKSYENEIQRIQLEAIEQQS+LNTKIQKIIDDNNQLKTEMQLELTNTQTNYERELKEKQSEIDKILVKRQMEIEKLNSEKIELQSNLDYTQEELKLINRKNQKILKDVKKQLEKEVKLTQETQSRLEEVVSSSSQNSQSKINEYLSGVHCKMSNNDDQSSLYSSPNTGIIDNCPLSSSPLNNIAMTDEELKILLDRFTSVQNDFCESKRIIESLHFDKSQLIQTIERQQSIILNYIKNRGKNDPEIDTDSPKH+HSTSEALILNGLMSSQTSNFGLQSVMNKLKDSLKSIASKDLMSLGDKTVCRVTTAEVKQLQRVCEELMMRCLNLENDLQLAIDMNTQLKTQMNAIL
Sbjct:    1 MENHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLERELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTECENLFSENERLKSQIETYKVTKTKQDVNINNINDSVDSPVHQDHEYKIRYLQHEVTKLLEEKTTIENKNEDLLKEIKIIKESENELINRINCLLNEKIKLANKCSVTEAEMNDISCNLSKQLEDQMNNYEMQLQSLKLGFAMDLEAIESKLKEEFSTKLDESTKSYENEIQRIQLEAIEQQSELNTKIQKIIDDNNQLKTEMQLELTNTQTNYERELKEKQSEIDKILVKRQMEIEKLNSEKIELQSNLDYTQEELKLINRKNQKILKDVKKQLEKEVKLTQETQSRLEEVVSSSSQNSQSKINEYLSGVHCKMSNNDDQSSLYSSPNTGIIDNCPLSSSPLNNIAMTDEELKILLDRFTSVQNDFCESKRIIESLHFDKSQLIQTIERQQSIILNYIKNRGKNDPEIDTDSPKHSHSTSEALILNGLMSSQTSNFGLQSVMNKLKDSLKSIASKDLMSLGDKTVCRVTTAEVKQLQRVCEELMMRCLNLENDLQLAIDMNTQLKTQMNAIL 612
The following BLAST results are available for this feature:
BLAST of GRIP1-associated protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GRIPAP16.689e-730.56GRIP1 associated protein 1 [Source:HGNC Symbol;Acc... [more]
GRIPAP16.719e-730.56GRIP1 associated protein 1 [Source:HGNC Symbol;Acc... [more]
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BLAST of GRIP1-associated protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gripap11.988e-832.43GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of GRIP1-associated protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Gripap12.606e-731.78GRIP1 associated protein 1 [Source:MGI Symbol;Acc:... [more]
Gripap12.707e-731.78GRIP1 associated protein 1 [Source:MGI Symbol;Acc:... [more]
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BLAST of GRIP1-associated protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q8VD04|GRAP1_MOUSE1.894e-631.78GRIP1-associated protein 1 OS=Mus musculus OX=1009... [more]
sp|Q4V328|GRAP1_HUMAN3.212e-630.56GRIP1-associated protein 1 OS=Homo sapiens OX=9606... [more]
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BLAST of GRIP1-associated protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S1BU462.000e-1139.67Uncharacterized protein (Fragment) OS=Elysia chlor... [more]
A0A564Z9A14.864e-1038.10Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0R3TZS41.605e-938.26Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A2J7QMV31.737e-938.18Uncharacterized protein (Fragment) OS=Cryptotermes... [more]
A0A210R7C12.959e-936.52GRIP1-associated protein 1 OS=Mizuhopecten yessoen... [more]
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BLAST of GRIP1-associated protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gripap12.351e-730.63GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GE... [more]
gripap12.466e-730.63GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GE... [more]
gripap12.687e-730.63GRIP1 associated protein 1 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of GRIP1-associated protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GRIP1-associated protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gripap11.688e-833.03GRIP1 associated protein 1 [Source:NCBI gene;Acc:1... [more]
gripap11.740e-833.03GRIP1 associated protein 1 [Source:NCBI gene;Acc:1... [more]
gripap11.779e-833.03GRIP1 associated protein 1 [Source:NCBI gene;Acc:1... [more]
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BLAST of GRIP1-associated protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000055144.10.000e+099.67SMESG000055144.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026701 ID=SMED30026701|Name=GRIP1-associated protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2032bp
ATTTTATTATTCCGTTGTATATTCTATTTTAAATTTCATATACGTTTGGT
AAATTTGAATGAATTAATTAAAGTAATTCAGAAAAGCAAAATTATTATTT
CTTTTCTTTATTTGGATTTTTTTTAAAATGGAAAATCATAGTATTACATC
AAGTGAATTTCAAAGATTTCAAGATCAACTAATATCATTAAAAGAAGCAA
AATATAAAATTGAGGAGAAATGTGAGTTTTTATTACGAAATTTATCTCAA
TCTGAAGTAAAATGCCAACATCTTGAAAGAGAACTATTGAAAGCAAATGA
TGTAAAAAACAAATTTAAATCTGCTAAAGACATGGACATTTTAATGAATG
AAAATCAAATTTTGCGTGACAGACTGAGTGCTATTGAAGGAAATTTCAAA
TTGCAAAATGACGTAATAAGAACTGAATGTGAAAATCTTTTTTCTGAAAA
TGAACGTCTCAAATCTCAAATTGAAACCTATAAAGTCACAAAAACCAAAC
AAGATGTGAATATTAATAATATTAATGATTCTGTCGATTCTCCTGTTCAT
CAAGATCACGAATATAAAATCAGATATCTCCAGCACGAAGTTACAAAATT
ATTGGAAGAAAAAACCACAATCGAAAACAAAAATGAAGATTTATTGAAAG
AAATTAAAATTATCAAAGAATCCGAAAATGAATTGATAAATAGAATTAAT
TGTTTATTGAATGAGAAGATAAAATTGGCCAATAAATGTTCTGTAACTGA
AGCGGAAATGAATGATATCAGTTGTAATTTGTCGAAACAATTAGAAGATC
AAATGAACAATTATGAAATGCAATTGCAATCATTGAAATTGGGATTCGCA
ATGGATCTAGAAGCAATTGAATCGAAACTGAAGGAAGAATTCTCAACAAA
GCTTGATGAGTCTACTAAAAGCTATGAAAATGAAATTCAAAGAATTCAAT
TGGAAGCAATCGAACAGCAATCCAAATTAAATACGAAAATCCAGAAAATT
ATTGACGATAACAATCAATTGAAAACGGAAATGCAATTAGAATTGACGAA
CACTCAAACGAATTACGAGAGGGAACTCAAGGAGAAACAGTCAGAAATCG
ATAAAATCCTCGTTAAACGTCAAATGGAAATCGAAAAATTGAATAGTGAG
AAAATCGAGTTGCAATCGAATCTTGATTATACTCAGGAGGAATTAAAATT
GATTAATCGAAAAAACCAGAAAATATTAAAGGATGTCAAAAAACAGCTGG
AGAAAGAAGTGAAATTGACTCAGGAAACCCAATCGAGATTGGAAGAAGTT
GTATCGAGCAGCTCACAGAACAGCCAATCGAAAATCAATGAGTATTTGAG
TGGAGTCCATTGCAAAATGTCGAATAATGACGATCAGAGCTCTCTTTATT
CTTCACCCAACACTGGAATTATCGATAATTGTCCGTTGTCGTCGTCGCCG
TTGAATAATATCGCAATGACCGATGAGGAGTTGAAGATTTTATTAGACAG
ATTTACAAGTGTACAAAATGATTTTTGTGAAAGCAAACGAATCATTGAGT
CTTTGCATTTTGATAAATCACAATTGATCCAAACCATAGAAAGACAACAA
AGTATCATTCTCAATTATATTAAGAACCGAGGCAAAAATGACCCCGAAAT
CGATACAGACTCTCCTAAACATAACCATTCCACGTCGGAAGCGCTGATTT
TGAATGGTTTAATGAGTTCCCAAACATCAAATTTCGGCCTTCAATCTGTG
ATGAATAAATTAAAAGACAGTTTGAAATCCATTGCTTCTAAAGATTTGAT
GTCGCTCGGTGACAAGACCGTTTGTCGCGTGACCACTGCAGAAGTAAAGC
AATTACAGAGGGTCTGTGAGGAGTTGATGATGCGTTGCTTGAATCTGGAA
AATGATCTCCAGCTAGCAATCGACATGAACACTCAACTGAAAACCCAAAT
GAATGCCATTCTGTGAAAGAGTTTTTATTTTTTATCAATCTATTCCATAG
TTTTAGCTTTATTCCATCCGTTGTTGTTGAGA
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protein sequence of SMED30026701-orf-1

>SMED30026701-orf-1 ID=SMED30026701-orf-1|Name=SMED30026701-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=613bp
MENHSITSSEFQRFQDQLISLKEAKYKIEEKCEFLLRNLSQSEVKCQHLE
RELLKANDVKNKFKSAKDMDILMNENQILRDRLSAIEGNFKLQNDVIRTE
CENLFSENERLKSQIETYKVTKTKQDVNINNINDSVDSPVHQDHEYKIRY
LQHEVTKLLEEKTTIENKNEDLLKEIKIIKESENELINRINCLLNEKIKL
ANKCSVTEAEMNDISCNLSKQLEDQMNNYEMQLQSLKLGFAMDLEAIESK
LKEEFSTKLDESTKSYENEIQRIQLEAIEQQSKLNTKIQKIIDDNNQLKT
EMQLELTNTQTNYERELKEKQSEIDKILVKRQMEIEKLNSEKIELQSNLD
YTQEELKLINRKNQKILKDVKKQLEKEVKLTQETQSRLEEVVSSSSQNSQ
SKINEYLSGVHCKMSNNDDQSSLYSSPNTGIIDNCPLSSSPLNNIAMTDE
ELKILLDRFTSVQNDFCESKRIIESLHFDKSQLIQTIERQQSIILNYIKN
RGKNDPEIDTDSPKHNHSTSEALILNGLMSSQTSNFGLQSVMNKLKDSLK
SIASKDLMSLGDKTVCRVTTAEVKQLQRVCEELMMRCLNLENDLQLAIDM
NTQLKTQMNAIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR026204GRIPAP1
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005654nucleoplasm
GO:0005768endosome
GO:0005769early endosome
GO:0005829cytosol
GO:0043231intracellular membrane-bounded organelle
GO:0072562blood microparticle
GO:0098837postsynaptic recycling endosome
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
GO:0098887neurotransmitter receptor transport, endosome to postsynaptic membrane
GO:1905244regulation of modification of synaptic structure
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 69..89
NoneNo IPR availableCOILSCoilCoilcoord: 263..301
NoneNo IPR availableCOILSCoilCoilcoord: 303..355
NoneNo IPR availableCOILSCoilCoilcoord: 155..189
NoneNo IPR availableCOILSCoilCoilcoord: 364..391
NoneNo IPR availableCOILSCoilCoilcoord: 32..59
NoneNo IPR availableCOILSCoilCoilcoord: 219..239
NoneNo IPR availableCOILSCoilCoilcoord: 573..593
NoneNo IPR availableCOILSCoilCoilcoord: 94..121
IPR026204GRIP1-associated protein 1PANTHERPTHR18978FAMILY NOT NAMEDcoord: 4..608