SMED30026642

Overview
NameSMED30026642
Smed IDSMED30026642
Length (bp)646
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30026642 (SMED30026642) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30026642 (SMED30026642) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30026642 (SMED30026642) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30026642 (SMED30026642) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30026642

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30026642SMESG000081274.1 SMESG000071708.1 SMESG000060519.1 SMESG000058103.1 SMESG000022037.1 SMESG000008378.1 SMESG000080313.1 SMESG000066935.1 SMESG000044742.1 Contig14429newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30026642SMESG000081274.1 SMESG000071708.1 SMESG000060519.1 SMESG000058103.1 SMESG000022037.1 SMESG000008378.1 SMESG000080313.1 SMESG000066935.1 SMESG000044742.1 Contig14429uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30026642SMESG000081199.1 SMESG000076404.1 SMESG000072273.1 SMESG000070010.1 SMESG000069658.1 SMESG000068312.1 SMESG000067818.1 SMESG000061623.1 SMESG000058180.1 SMESG000057612.1 SMESG000056465.1 SMESG000051141.1 SMESG000044801.1 SMESG000043241.1 SMESG000042662.1 SMESG000039424.1 SMESG000033772.1 SMESG000033494.1 SMESG000033266.1 SMESG000022351.1 SMESG000019964.1 SMESG000012636.1 SMESG000010351.1 SMESG000009500.1 SMESG000008761.1 SMESG000005685.1 SMESG000002786.1 SMESG000001786.1 SMESG000040856.1 Contig16275newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30026642SMESG000081199.1 SMESG000076404.1 SMESG000072273.1 SMESG000070010.1 SMESG000069658.1 SMESG000068312.1 SMESG000067818.1 SMESG000061623.1 SMESG000058180.1 SMESG000057612.1 SMESG000056465.1 SMESG000051141.1 SMESG000044801.1 SMESG000043241.1 SMESG000042662.1 SMESG000039424.1 SMESG000033772.1 SMESG000033494.1 SMESG000033266.1 SMESG000022351.1 SMESG000019964.1 SMESG000012636.1 SMESG000010351.1 SMESG000009500.1 SMESG000008761.1 SMESG000005685.1 SMESG000002786.1 SMESG000001786.1 SMESG000040856.1 Contig16275uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30026642SMESG000081199.1 SMESG000076404.1 SMESG000072273.1 SMESG000070010.1 SMESG000069658.1 SMESG000068312.1 SMESG000067818.1 SMESG000061623.1 SMESG000058180.1 SMESG000057612.1 SMESG000056465.1 SMESG000051141.1 SMESG000044801.1 SMESG000043241.1 SMESG000042662.1 SMESG000039424.1 SMESG000033772.1 SMESG000033494.1 SMESG000033266.1 SMESG000022351.1 SMESG000019964.1 SMESG000012636.1 SMESG000010351.1 SMESG000009500.1 SMESG000008761.1 SMESG000005685.1 SMESG000002786.1 SMESG000001786.1 SMESG000040856.1 Contig16275newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30026642SMESG000081199.1 SMESG000076404.1 SMESG000072273.1 SMESG000070010.1 SMESG000069658.1 SMESG000068312.1 SMESG000067818.1 SMESG000061623.1 SMESG000058180.1 SMESG000057612.1 SMESG000056465.1 SMESG000051141.1 SMESG000044801.1 SMESG000043241.1 SMESG000042662.1 SMESG000039424.1 SMESG000033772.1 SMESG000033494.1 SMESG000033266.1 SMESG000022351.1 SMESG000019964.1 SMESG000012636.1 SMESG000010351.1 SMESG000009500.1 SMESG000008761.1 SMESG000005685.1 SMESG000002786.1 SMESG000001786.1 SMESG000040856.1 Contig16275uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30026642SMESG000074134.1 SMESG000051558.1 SMESG000033261.1 SMESG000013659.1 SMESG000075824.1 Contig30255newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30026642SMESG000074134.1 SMESG000051558.1 SMESG000033261.1 SMESG000013659.1 SMESG000075824.1 Contig30255uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30026642 vs. TrEMBL
Match: A0A3C1P7M4 (Uncharacterized protein OS=Planktothrix sp. UBA8402 OX=2055759 GN=DCQ63_02390 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.239e-7
Identity = 37/86 (43.02%), Postives = 59/86 (68.60%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYE----QGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRKI 247
            IY++   P+ VG  RVPLLK+I+V   +    QG +  L ++NPMY+ ++ +T+N+IEIN+R+D+G +I F K   T L++HF+K 
Sbjct:  288 IYASCCAPVYVGHTRVPLLKNIFVDASQDANIQGHVRNLSIQNPMYVNVAATTLNSIEINMRNDAGNIIGFPKGAITVLTLHFKKF 373
[Close]
BLAST of SMED30026642 vs. TrEMBL
Match: A0A0J7K458 (Uncharacterized protein OS=Lasius niger OX=67767 GN=RF55_16786 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.474e-6
Identity = 33/80 (41.25%), Postives = 50/80 (62.50%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFR 241
            +Y +++EP  VGDV+ PLLK + VK ++ GEI+ +    P+YLPI       I ++IR  SG L+ F +  K  L++HFR
Sbjct:  166 VYCDLVEPQIVGDVQAPLLKIVNVKGHD-GEIININFPRPVYLPIIRQHFQTIHVDIRQHSGALVPF-ESGKVFLTLHFR 243
[Close]
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000019964.1 (SMESG000019964.1)

HSP 1 Score: 256.914 bits (655), Expect = 3.294e-94
Identity = 125/129 (96.90%), Postives = 126/129 (97.67%), Query Frame = 3 
Query:  258 EFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            +FYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLH SYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI
Sbjct:  649 KFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHKSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 777

HSP 2 Score: 107.071 bits (266), Expect = 3.294e-94
Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKF 202
            IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQL+MKNPMYLPISTSTINNIEINIRDDSGKLIKF
Sbjct:  582 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLKMKNPMYLPISTSTINNIEINIRDDSGKLIKF 648

HSP 3 Score: 109.383 bits (272), Expect = 2.459e-27
Identity = 65/153 (42.48%), Postives = 87/153 (56.86%), Query Frame = 3 
Query:  186 EN*LNLVKIPK--HIYQYILEK*MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTI--SHDSIFYLVYIDG-NTLNINCKYFPFKILFDSIAEAQKLGF 629
            E  L +++ P    I + +  K +  +FY+ LPSNSC LT PNN+A  FNVEFQNPIYL+G+W+VAL DFTFVY  FPFY+NS + Y      T  +Y   N+    Y +  S  +    V+I+    L I C + PFK+ F  I  A   G 
Sbjct:  305 EQELQVIEAPTVFRIQKILKTKNVENQFYMILPSNSCPLTFPNNEASNFNVEFQNPIYLDGDWEVALIDFTFVYKTFPFYSNSYLSYETIKSGTEVKYLKINNQNETYELINSKHTPDVDVFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGL 457

HSP 4 Score: 78.9518 bits (193), Expect = 7.104e-17
Identity = 48/80 (60.00%), Postives = 65/80 (81.25%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFR 241
            IY+NIIEP+ VGDV +PLLK +WV+ +++ ++VQ+  KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HF 
Sbjct:  901 IYTNIIEPVLVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLSVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFH 979
[Close]
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000019964.1 (SMESG000019964.1)

HSP 1 Score: 256.529 bits (654), Expect = 3.858e-94
Identity = 125/129 (96.90%), Postives = 126/129 (97.67%), Query Frame = 3 
Query:  258 EFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            +FYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLH SYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI
Sbjct:  649 KFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHKSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 777

HSP 2 Score: 106.686 bits (265), Expect = 3.858e-94
Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKF 202
            IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQL+MKNPMYLPISTSTINNIEINIRDDSGKLIKF
Sbjct:  582 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLKMKNPMYLPISTSTINNIEINIRDDSGKLIKF 648

HSP 3 Score: 109.383 bits (272), Expect = 2.232e-27
Identity = 65/153 (42.48%), Postives = 87/153 (56.86%), Query Frame = 3 
Query:  186 EN*LNLVKIPK--HIYQYILEK*MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTI--SHDSIFYLVYIDG-NTLNINCKYFPFKILFDSIAEAQKLGF 629
            E  L +++ P    I + +  K +  +FY+ LPSNSC LT PNN+A  FNVEFQNPIYL+G+W+VAL DFTFVY  FPFY+NS + Y      T  +Y   N+    Y +  S  +    V+I+    L I C + PFK+ F  I  A   G 
Sbjct:  305 EQELQVIEAPTVFRIQKILKTKNVENQFYMILPSNSCPLTFPNNEASNFNVEFQNPIYLDGDWEVALIDFTFVYKTFPFYSNSYLSYETIKSGTEVKYLKINNQNETYELINSKHTPDVDVFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGL 457

HSP 4 Score: 67.3958 bits (163), Expect = 6.589e-13
Identity = 41/65 (63.08%), Postives = 55/65 (84.62%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLI 196
            IY+NIIEP+ VGDV +PLLK +WV+ +++ ++VQ+  KNPMYL +S++ INNIEINIRDDSGK I
Sbjct:  901 IYTNIIEPVLVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLSVSSTYINNIEINIRDDSGKYI 965
[Close]
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000033494.1 (SMESG000033494.1)

HSP 1 Score: 265.388 bits (677), Expect = 1.091e-81
Identity = 129/132 (97.73%), Postives = 129/132 (97.73%), Query Frame = 3 
Query:  249 MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            MNKEFYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDA NEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI
Sbjct:    1 MNKEFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDANNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 132

HSP 2 Score: 83.1889 bits (204), Expect = 3.254e-18
Identity = 51/81 (62.96%), Postives = 67/81 (82.72%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRK 244
            IY+NIIEPI VGDV +PLLK +WV+ +++ ++VQ+  KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HFRK
Sbjct:  256 IYTNIIEPILVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLYVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFRK 335
[Close]
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000033494.1 (SMESG000033494.1)

HSP 1 Score: 265.388 bits (677), Expect = 1.203e-81
Identity = 129/132 (97.73%), Postives = 129/132 (97.73%), Query Frame = 3 
Query:  249 MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            MNKEFYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDA NEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI
Sbjct:    1 MNKEFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDANNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 132

HSP 2 Score: 82.8037 bits (203), Expect = 3.351e-18
Identity = 51/81 (62.96%), Postives = 67/81 (82.72%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRK 244
            IY+NIIEPI VGDV +PLLK +WV+ +++ ++VQ+  KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HFRK
Sbjct:  256 IYTNIIEPILVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLYVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFRK 335
[Close]
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000059936.1 (SMESG000059936.1)

HSP 1 Score: 182.956 bits (463), Expect = 1.366e-76
Identity = 91/122 (74.59%), Postives = 102/122 (83.61%), Query Frame = 3 
Query:  279 SNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            SNSC LT PNN+A KFNVEF N IYL+GEW+VALT+FTFVYNNFPFYNNS IKY+KSTLH SYEYFYY   +  YTISH+S FYL+YID   L+INCKYFPFK+ FDSI EA  LGFNKKE+
Sbjct:  613 SNSCPLTFPNNEAGKFNVEFPNQIYLDGEWEVALTEFTFVYNNFPFYNNSCIKYTKSTLHNSYEYFYY--IEGNYTISHNSTFYLIYIDNKILSINCKYFPFKVTFDSIEEANCLGFNKKEV 732

HSP 2 Score: 121.709 bits (304), Expect = 1.366e-76
Identity = 68/76 (89.47%), Postives = 73/76 (96.05%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLS 229
            IYSNIIEPI VGDVRVPLLKS+W+KNY+QGEIVQLEMKNPMYLP+S STIN I+INIRDDSGKLIKFSKDTKTHLS
Sbjct:  538 IYSNIIEPINVGDVRVPLLKSVWIKNYDQGEIVQLEMKNPMYLPVSYSTINIIQINIRDDSGKLIKFSKDTKTHLS 613

HSP 3 Score: 65.0846 bits (157), Expect = 4.480e-12
Identity = 41/65 (63.08%), Postives = 54/65 (83.08%), Query Frame = 2 
Query:    2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLI 196
            IY+NIIEPI VG++ V LLK +W++ +++ ++VQ   KNPMYLP+S+S INNIEINIRDDSGK I
Sbjct:  854 IYTNIIEPILVGELSVLLLKCVWIEKHKKDDLVQFIEKNPMYLPVSSSYINNIEINIRDDSGKYI 918

HSP 4 Score: 56.6102 bits (135), Expect = 3.242e-9
Identity = 36/102 (35.29%), Postives = 50/102 (49.02%), Query Frame = 3 
Query:  354 LEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDG-----NTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644
            ++GEW+VAL DFTFVYN F +Y+NS I+Y      +  +Y   N+  + Y I +    Y   +D        L I C + PFK+ F  I  A   G     I
Sbjct:  316 VDGEWEVALIDFTFVYNPFTYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPK--YTPDVDMFIEKYENLVIVCPHVPFKVTFLDIQHAFSFGLKSTSI 415
The following BLAST results are available for this feature:
BLAST of SMED30026642 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A3C1P7M41.239e-743.02Uncharacterized protein OS=Planktothrix sp. UBA840... [more]
A0A0J7K4585.474e-641.25Uncharacterized protein OS=Lasius niger OX=67767 G... [more]
back to top
BLAST of SMED30026642 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30026642 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000019964.13.294e-9496.90SMESG000019964.1[more]
SMESG000019964.13.858e-9496.90SMESG000019964.1[more]
SMESG000033494.11.091e-8197.73SMESG000033494.1[more]
SMESG000033494.11.203e-8197.73SMESG000033494.1[more]
SMESG000059936.11.366e-7674.59SMESG000059936.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026642 ID=SMED30026642|Name=SMED30026642|organism=Schmidtea mediterranea sexual|type=transcript|length=646bp
CATTTATTCGAATATTATTGAACCAATAACTGTTGGAGATGTTAGAGTTC
CTCTATTAAAATCTATTTGGGTTAAAAACTATGAACAAGGGGAAATTGTA
CAATTAGAAATGAAGAATCCAATGTATTTACCAATTTCAACTTCAACTAT
AAACAACATTGAAATTAATATTCGCGATGATAGTGGAAAATTAATTAAAT
TTAGTAAAGATACCAAAACACATTTATCAATACATTTTAGAAAAATAAAT
GAATAAAGAATTTTACATAAATCTACCGAGTAATAGTTGTCATCTTACTC
ATCCAAATAATAAAGCTGATAAATTTAATGTTGAATTTCAAAATCCAATT
TACTTGGAAGGTGAATGGCAAGTTGCTCTGACCGATTTCACTTTTGTATA
CAATAATTTTCCATTTTATAACAACTCAAATATTAAATATAGTAAGAGTA
CATTACATACAAGTTATGAATATTTTTACTATAACGATGCAAAGAATGAA
TATACAATTAGTCACGACTCTATATTTTATCTAGTATACATTGATGGAAA
TACTTTAAATATTAATTGTAAATATTTTCCATTTAAAATTTTATTTGACA
GTATTGCAGAAGCTCAAAAATTAGGATTTAATAAAAAAGAAATAAA
back to top

protein sequence of SMED30026642-orf-1

>SMED30026642-orf-1 ID=SMED30026642-orf-1|Name=SMED30026642-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFV
YNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDG
NTLNINCKYFPFKILFDSIAEAQKLGFNKKEI
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
PLANA:0002089reproductive organ