SMED30026642
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026642 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30026642 vs. TrEMBL
Match: A0A3C1P7M4 (Uncharacterized protein OS=Planktothrix sp. UBA8402 OX=2055759 GN=DCQ63_02390 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 1.239e-7 Identity = 37/86 (43.02%), Postives = 59/86 (68.60%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYE----QGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRKI 247 IY++ P+ VG RVPLLK+I+V + QG + L ++NPMY+ ++ +T+N+IEIN+R+D+G +I F K T L++HF+K Sbjct: 288 IYASCCAPVYVGHTRVPLLKNIFVDASQDANIQGHVRNLSIQNPMYVNVAATTLNSIEINMRNDAGNIIGFPKGAITVLTLHFKKF 373
BLAST of SMED30026642 vs. TrEMBL
Match: A0A0J7K458 (Uncharacterized protein OS=Lasius niger OX=67767 GN=RF55_16786 PE=4 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 5.474e-6 Identity = 33/80 (41.25%), Postives = 50/80 (62.50%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFR 241 +Y +++EP VGDV+ PLLK + VK ++ GEI+ + P+YLPI I ++IR SG L+ F + K L++HFR Sbjct: 166 VYCDLVEPQIVGDVQAPLLKIVNVKGHD-GEIININFPRPVYLPIIRQHFQTIHVDIRQHSGALVPF-ESGKVFLTLHFR 243
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000019964.1 (SMESG000019964.1) HSP 1 Score: 256.914 bits (655), Expect = 3.294e-94 Identity = 125/129 (96.90%), Postives = 126/129 (97.67%), Query Frame = 3 Query: 258 EFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 +FYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLH SYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI Sbjct: 649 KFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHKSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 777 HSP 2 Score: 107.071 bits (266), Expect = 3.294e-94 Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKF 202 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQL+MKNPMYLPISTSTINNIEINIRDDSGKLIKF Sbjct: 582 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLKMKNPMYLPISTSTINNIEINIRDDSGKLIKF 648 HSP 3 Score: 109.383 bits (272), Expect = 2.459e-27 Identity = 65/153 (42.48%), Postives = 87/153 (56.86%), Query Frame = 3 Query: 186 EN*LNLVKIPK--HIYQYILEK*MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTI--SHDSIFYLVYIDG-NTLNINCKYFPFKILFDSIAEAQKLGF 629 E L +++ P I + + K + +FY+ LPSNSC LT PNN+A FNVEFQNPIYL+G+W+VAL DFTFVY FPFY+NS + Y T +Y N+ Y + S + V+I+ L I C + PFK+ F I A G Sbjct: 305 EQELQVIEAPTVFRIQKILKTKNVENQFYMILPSNSCPLTFPNNEASNFNVEFQNPIYLDGDWEVALIDFTFVYKTFPFYSNSYLSYETIKSGTEVKYLKINNQNETYELINSKHTPDVDVFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGL 457 HSP 4 Score: 78.9518 bits (193), Expect = 7.104e-17 Identity = 48/80 (60.00%), Postives = 65/80 (81.25%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFR 241 IY+NIIEP+ VGDV +PLLK +WV+ +++ ++VQ+ KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HF Sbjct: 901 IYTNIIEPVLVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLSVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFH 979
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000019964.1 (SMESG000019964.1) HSP 1 Score: 256.529 bits (654), Expect = 3.858e-94 Identity = 125/129 (96.90%), Postives = 126/129 (97.67%), Query Frame = 3 Query: 258 EFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 +FYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLH SYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI Sbjct: 649 KFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHKSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 777 HSP 2 Score: 106.686 bits (265), Expect = 3.858e-94 Identity = 66/67 (98.51%), Postives = 67/67 (100.00%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKF 202 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQL+MKNPMYLPISTSTINNIEINIRDDSGKLIKF Sbjct: 582 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLKMKNPMYLPISTSTINNIEINIRDDSGKLIKF 648 HSP 3 Score: 109.383 bits (272), Expect = 2.232e-27 Identity = 65/153 (42.48%), Postives = 87/153 (56.86%), Query Frame = 3 Query: 186 EN*LNLVKIPK--HIYQYILEK*MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTI--SHDSIFYLVYIDG-NTLNINCKYFPFKILFDSIAEAQKLGF 629 E L +++ P I + + K + +FY+ LPSNSC LT PNN+A FNVEFQNPIYL+G+W+VAL DFTFVY FPFY+NS + Y T +Y N+ Y + S + V+I+ L I C + PFK+ F I A G Sbjct: 305 EQELQVIEAPTVFRIQKILKTKNVENQFYMILPSNSCPLTFPNNEASNFNVEFQNPIYLDGDWEVALIDFTFVYKTFPFYSNSYLSYETIKSGTEVKYLKINNQNETYELINSKHTPDVDVFIEKYENLVIVCPHVPFKVTFPDIQHAFSFGL 457 HSP 4 Score: 67.3958 bits (163), Expect = 6.589e-13 Identity = 41/65 (63.08%), Postives = 55/65 (84.62%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLI 196 IY+NIIEP+ VGDV +PLLK +WV+ +++ ++VQ+ KNPMYL +S++ INNIEINIRDDSGK I Sbjct: 901 IYTNIIEPVLVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLSVSSTYINNIEINIRDDSGKYI 965
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000033494.1 (SMESG000033494.1) HSP 1 Score: 265.388 bits (677), Expect = 1.091e-81 Identity = 129/132 (97.73%), Postives = 129/132 (97.73%), Query Frame = 3 Query: 249 MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 MNKEFYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDA NEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI Sbjct: 1 MNKEFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDANNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 132 HSP 2 Score: 83.1889 bits (204), Expect = 3.254e-18 Identity = 51/81 (62.96%), Postives = 67/81 (82.72%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRK 244 IY+NIIEPI VGDV +PLLK +WV+ +++ ++VQ+ KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HFRK Sbjct: 256 IYTNIIEPILVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLYVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFRK 335
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000033494.1 (SMESG000033494.1) HSP 1 Score: 265.388 bits (677), Expect = 1.203e-81 Identity = 129/132 (97.73%), Postives = 129/132 (97.73%), Query Frame = 3 Query: 249 MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 MNKEFYINLPSNSC LTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDA NEYTISHDSIFYLVYIDGNTLNINCKYFPFKI FDSIAEAQKLGFNKKEI Sbjct: 1 MNKEFYINLPSNSCLLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDANNEYTISHDSIFYLVYIDGNTLNINCKYFPFKISFDSIAEAQKLGFNKKEI 132 HSP 2 Score: 82.8037 bits (203), Expect = 3.351e-18 Identity = 51/81 (62.96%), Postives = 67/81 (82.72%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLSIHFRK 244 IY+NIIEPI VGDV +PLLK +WV+ +++ ++VQ+ KNPMYL +S++ INNIEINIRDDSGK I F K+ KT L++HFRK Sbjct: 256 IYTNIIEPILVGDVLIPLLKCVWVEKHKKDDLVQIIEKNPMYLYVSSTYINNIEINIRDDSGKYI-FGKEDKTFLTLHFRK 335
BLAST of SMED30026642 vs. Planmine SMEST
Match: SMESG000059936.1 (SMESG000059936.1) HSP 1 Score: 182.956 bits (463), Expect = 1.366e-76 Identity = 91/122 (74.59%), Postives = 102/122 (83.61%), Query Frame = 3 Query: 279 SNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDGNTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 SNSC LT PNN+A KFNVEF N IYL+GEW+VALT+FTFVYNNFPFYNNS IKY+KSTLH SYEYFYY + YTISH+S FYL+YID L+INCKYFPFK+ FDSI EA LGFNKKE+ Sbjct: 613 SNSCPLTFPNNEAGKFNVEFPNQIYLDGEWEVALTEFTFVYNNFPFYNNSCIKYTKSTLHNSYEYFYY--IEGNYTISHNSTFYLIYIDNKILSINCKYFPFKVTFDSIEEANCLGFNKKEV 732 HSP 2 Score: 121.709 bits (304), Expect = 1.366e-76 Identity = 68/76 (89.47%), Postives = 73/76 (96.05%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLIKFSKDTKTHLS 229 IYSNIIEPI VGDVRVPLLKS+W+KNY+QGEIVQLEMKNPMYLP+S STIN I+INIRDDSGKLIKFSKDTKTHLS Sbjct: 538 IYSNIIEPINVGDVRVPLLKSVWIKNYDQGEIVQLEMKNPMYLPVSYSTINIIQINIRDDSGKLIKFSKDTKTHLS 613 HSP 3 Score: 65.0846 bits (157), Expect = 4.480e-12 Identity = 41/65 (63.08%), Postives = 54/65 (83.08%), Query Frame = 2 Query: 2 IYSNIIEPITVGDVRVPLLKSIWVKNYEQGEIVQLEMKNPMYLPXXXXXXXXXXXXXRDDSGKLI 196 IY+NIIEPI VG++ V LLK +W++ +++ ++VQ KNPMYLP+S+S INNIEINIRDDSGK I Sbjct: 854 IYTNIIEPILVGELSVLLLKCVWIEKHKKDDLVQFIEKNPMYLPVSSSYINNIEINIRDDSGKYI 918 HSP 4 Score: 56.6102 bits (135), Expect = 3.242e-9 Identity = 36/102 (35.29%), Postives = 50/102 (49.02%), Query Frame = 3 Query: 354 LEGEWQVALTDFTFVYNNFPFYNNSNIKYSKSTLHTSYEYFYYNDAKNEYTISHDSIFYLVYIDG-----NTLNINCKYFPFKILFDSIAEAQKLGFNKKEI 644 ++GEW+VAL DFTFVYN F +Y+NS I+Y + +Y N+ + Y I + Y +D L I C + PFK+ F I A G I Sbjct: 316 VDGEWEVALIDFTFVYNPFTYYSNSCIRYESVKSGSEIKYLKINNQNHTYEIINPK--YTPDVDMFIEKYENLVIVCPHVPFKVTFLDIQHAFSFGLKSTSI 415 The following BLAST results are available for this feature:
BLAST of SMED30026642 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30026642 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30026642 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of SMED30026642 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30026642 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30026642 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026642 ID=SMED30026642|Name=SMED30026642|organism=Schmidtea mediterranea sexual|type=transcript|length=646bpback to top protein sequence of SMED30026642-orf-1 >SMED30026642-orf-1 ID=SMED30026642-orf-1|Name=SMED30026642-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp MNKEFYINLPSNSCHLTHPNNKADKFNVEFQNPIYLEGEWQVALTDFTFVback to top Annotated Terms
The following terms have been associated with this transcript:
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