Intermediate filament protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026612 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 46Note: Hover over icons to view figure legend
Homology
BLAST of Intermediate filament protein vs. Ensembl Human
Match: VIM (vimentin [Source:HGNC Symbol;Acc:HGNC:12692]) HSP 1 Score: 121.709 bits (304), Expect = 9.237e-29 Identity = 116/393 (29.52%), Postives = 203/393 (51.65%), Query Frame = 2
Query: 422 NIPLIR--GDSV--ALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
++P +R DSV +L + T K R EK +LQ+LN+RFA+YI+K RFLE QNK L+ E+E+LKG Q S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L Q + +E+ E + L+ RQD + +LA L LE +++SL+E + F K++H+ E++EL + ++ + ++ ALRD++ +YE+ QE + Y S+ ++ + R+N K+E++ + Q+QS+ + L+ N E + + N+ + + + LQ+++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ NFSS
Sbjct: 73 SVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG---QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKL-------------------------------------QEEMLQREEAE-----NTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSS 420
BLAST of Intermediate filament protein vs. Ensembl Human
Match: VIM (vimentin [Source:HGNC Symbol;Acc:HGNC:12692]) HSP 1 Score: 121.324 bits (303), Expect = 1.689e-28 Identity = 116/393 (29.52%), Postives = 206/393 (52.42%), Query Frame = 2
Query: 422 NIPLIR--GDSV--ALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
++P +R DSV +L + T K R EK +LQ+LN+RFA+YI+K RFLE QNK L+ E+E+LKG Q S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L + E+++ +EE + + L+ RQD + +LA L LE +++SL+E + F K++H+ E++EL + ++ + ++ ALRD++ +YE+ QE + Y S+ ++ + R+N K+E++ + Q+QS+ + L+ N E + + N+ + + + LQ+++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ NFSS
Sbjct: 73 SVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG---QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKL------------------------QEEMLQ---------------REEAE---NTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSS 420
BLAST of Intermediate filament protein vs. Ensembl Human
Match: VIM (vimentin [Source:HGNC Symbol;Acc:HGNC:12692]) HSP 1 Score: 121.324 bits (303), Expect = 1.689e-28 Identity = 116/393 (29.52%), Postives = 206/393 (52.42%), Query Frame = 2
Query: 422 NIPLIR--GDSV--ALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
++P +R DSV +L + T K R EK +LQ+LN+RFA+YI+K RFLE QNK L+ E+E+LKG Q S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L + E+++ +EE + + L+ RQD + +LA L LE +++SL+E + F K++H+ E++EL + ++ + ++ ALRD++ +YE+ QE + Y S+ ++ + R+N K+E++ + Q+QS+ + L+ N E + + N+ + + + LQ+++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ NFSS
Sbjct: 73 SVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG---QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKL------------------------QEEMLQ---------------REEAE---NTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSS 420
BLAST of Intermediate filament protein vs. Ensembl Human
Match: GFAP (glial fibrillary acidic protein [Source:HGNC Symbol;Acc:HGNC:4235]) HSP 1 Score: 107.071 bits (266), Expect = 1.141e-23 Identity = 102/372 (27.42%), Postives = 179/372 (48.12%), Query Frame = 2
Query: 452 ALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
AL +G K+ RA E+ ++ +LN+RFASYIEK RFLE QNK L E+ +L+ ++ +K+ ++Y+ E+ +LR L + A LEV+ D+L ++ +R++L ++ N + ++ E L R +E +E R DL++ +++SL+E + F ++IH+ EV+EL R + + ++ AL++I+ +YE + E + Y S+ ++ + R+ K E + + Q+QS+ + L N E + + S + L L+E+ + ++ L E Q LL+ KL L++EIA Y KLLE EENR+
Sbjct: 57 ALNAG---FKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRA---KEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQ--DETNLRLEA------------ENNLAAYR-----------QEADEATLARLDLER-----------------KIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIP 380
BLAST of Intermediate filament protein vs. Ensembl Human
Match: GFAP (glial fibrillary acidic protein [Source:HGNC Symbol;Acc:HGNC:4235]) HSP 1 Score: 106.301 bits (264), Expect = 1.217e-23 Identity = 99/372 (26.61%), Postives = 177/372 (47.58%), Query Frame = 2
Query: 452 ALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
AL +G K+ RA E+ ++ +LN+RFASYIEK RFLE QNK L E+ +L+ ++ +K+ ++Y+ E+ +LR L + A LEV+ D+L ++ +R++L ++ + ++ + A +E +E R DL++ +++SL+E + F ++IH+ EV+EL R + + ++ AL++I+ +YE + E + Y S+ ++ + R+ K E + + Q+QS+ + L N E + + S + L L+E+ + ++ L E Q LL+ KL L++EIA Y KLLE EENR+
Sbjct: 57 ALNAG---FKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRA---KEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNL-------------------------AAYRQEADEATLARLDLER-----------------KIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIP 380
BLAST of Intermediate filament protein vs. Ensembl Celegans
Match: ifa-1 (Intermediate filament protein ifa-1 [Source:UniProtKB/Swiss-Prot;Acc:P90901]) HSP 1 Score: 188.348 bits (477), Expect = 4.782e-51 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 56 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 424 HSP 2 Score: 73.1738 bits (178), Expect = 3.803e-13 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 456 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
BLAST of Intermediate filament protein vs. Ensembl Celegans
Match: ifa-1 (Intermediate filament protein ifa-1 [Source:UniProtKB/Swiss-Prot;Acc:P90901]) HSP 1 Score: 188.348 bits (477), Expect = 5.168e-51 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 56 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 424 HSP 2 Score: 73.1738 bits (178), Expect = 3.770e-13 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 456 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
BLAST of Intermediate filament protein vs. Ensembl Celegans
Match: ifa-1 (Intermediate filament protein ifa-1 [Source:UniProtKB/Swiss-Prot;Acc:P90901]) HSP 1 Score: 187.963 bits (476), Expect = 6.922e-51 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 48 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 416 HSP 2 Score: 73.1738 bits (178), Expect = 4.156e-13 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 448 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 562
BLAST of Intermediate filament protein vs. Ensembl Celegans
Match: ifa-1 (Intermediate filament protein ifa-1 [Source:UniProtKB/Swiss-Prot;Acc:P90901]) HSP 1 Score: 187.963 bits (476), Expect = 6.922e-51 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 48 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 416 HSP 2 Score: 73.1738 bits (178), Expect = 4.156e-13 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 448 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 562
BLAST of Intermediate filament protein vs. Ensembl Celegans
Match: ifa-1 (Intermediate filament protein ifa-1 [Source:UniProtKB/Swiss-Prot;Acc:P90901]) HSP 1 Score: 187.963 bits (476), Expect = 6.922e-51 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 48 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 416 HSP 2 Score: 73.1738 bits (178), Expect = 4.156e-13 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 448 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 562
BLAST of Intermediate filament protein vs. Ensembl Fly
Match: Lam (gene:FBgn0002525 transcript:FBtr0346460) HSP 1 Score: 119.013 bits (297), Expect = 1.825e-27 Identity = 108/392 (27.55%), Postives = 205/392 (52.30%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELV-------ELRRFKGTQGA-------DVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKE---LATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTS 1585
S ++ + R EK +LQ+LN+R A+YI++ R LE +N +L E++ + ++ + IK I+E E+ + R LL D+ + RA E+ + L E N + K+ +DK+ K+ + EG + EL A D+N+ +E++ LR ++ R++ E+E L+ + LE+ +QSL+E L F+ QIH E+ E + Y + + +++ ++L++++ +YE ++ + E+ S Y ++Q +Q + R + T EE + +I ++ + I +LE NA+ + ++ + + ++ L+++L ++++T L E Q L+D K+ L+LEIAAY KLL EE R+N + NT+
Sbjct: 42 SPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEE--------------NEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTA 419
BLAST of Intermediate filament protein vs. Ensembl Fly
Match: Lam (gene:FBgn0002525 transcript:FBtr0307059) HSP 1 Score: 119.013 bits (297), Expect = 1.825e-27 Identity = 108/392 (27.55%), Postives = 205/392 (52.30%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELV-------ELRRFKGTQGA-------DVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKE---LATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTS 1585
S ++ + R EK +LQ+LN+R A+YI++ R LE +N +L E++ + ++ + IK I+E E+ + R LL D+ + RA E+ + L E N + K+ +DK+ K+ + EG + EL A D+N+ +E++ LR ++ R++ E+E L+ + LE+ +QSL+E L F+ QIH E+ E + Y + + +++ ++L++++ +YE ++ + E+ S Y ++Q +Q + R + T EE + +I ++ + I +LE NA+ + ++ + + ++ L+++L ++++T L E Q L+D K+ L+LEIAAY KLL EE R+N + NT+
Sbjct: 42 SPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEE--------------NEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTA 419
BLAST of Intermediate filament protein vs. Ensembl Fly
Match: Lam (gene:FBgn0002525 transcript:FBtr0307058) HSP 1 Score: 119.013 bits (297), Expect = 1.825e-27 Identity = 108/392 (27.55%), Postives = 205/392 (52.30%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELV-------ELRRFKGTQGA-------DVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKE---LATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTS 1585
S ++ + R EK +LQ+LN+R A+YI++ R LE +N +L E++ + ++ + IK I+E E+ + R LL D+ + RA E+ + L E N + K+ +DK+ K+ + EG + EL A D+N+ +E++ LR ++ R++ E+E L+ + LE+ +QSL+E L F+ QIH E+ E + Y + + +++ ++L++++ +YE ++ + E+ S Y ++Q +Q + R + T EE + +I ++ + I +LE NA+ + ++ + + ++ L+++L ++++T L E Q L+D K+ L+LEIAAY KLL EE R+N + NT+
Sbjct: 42 SPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEE--------------NEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTA 419
BLAST of Intermediate filament protein vs. Ensembl Fly
Match: Lam (gene:FBgn0002525 transcript:FBtr0079100) HSP 1 Score: 119.013 bits (297), Expect = 1.825e-27 Identity = 108/392 (27.55%), Postives = 205/392 (52.30%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELV-------ELRRFKGTQGA-------DVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKE---LATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTS 1585
S ++ + R EK +LQ+LN+R A+YI++ R LE +N +L E++ + ++ + IK I+E E+ + R LL D+ + RA E+ + L E N + K+ +DK+ K+ + EG + EL A D+N+ +E++ LR ++ R++ E+E L+ + LE+ +QSL+E L F+ QIH E+ E + Y + + +++ ++L++++ +YE ++ + E+ S Y ++Q +Q + R + T EE + +I ++ + I +LE NA+ + ++ + + ++ L+++L ++++T L E Q L+D K+ L+LEIAAY KLL EE R+N + NT+
Sbjct: 42 SPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEE--------------NEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTA 419
BLAST of Intermediate filament protein vs. Ensembl Fly
Match: LamC (gene:FBgn0010397 transcript:FBtr0087512) HSP 1 Score: 110.538 bits (275), Expect = 1.054e-24 Identity = 109/392 (27.81%), Postives = 209/392 (53.32%), Query Frame = 2
Query: 434 IRGDSVALQSGVTS----MKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRL--MMLEDINSQYKSTIDKQR-KDISG-FEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELAT---VAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDG 1576
+ G S + + G TS + R QEK +LQ LN+R A YI++ R LE +N +L E+ + ++ S +K +YE E+ R LL ++ K +A LE+ + L E DDL+ RL E ++ + + + R +++G + L + ++F+ Q ++ E E LR L LR+ E E LA + LE++ QSL+E L F+ Q+H E+ E + + + +++++ ++L++++++YE ++ ++E++ Y +++Q ++ + R + EE ++++I + +++ +LE NA + + + ++ + +L+ +L + ++ L E Q L+D K+ L+LEIAAY KLL EE R+N S G
Sbjct: 21 VGGASTSSRVGATSPTSPTRTSRQQEKEELQHLNDRLACYIDRMRNLENENSRLTQELNLAQDTVNRETSNLKAVYEKELAAARKLLDETAKEKAKLEIDIKRLWEENDDLKPRLDKKTKEATVAENNARLYENRYNEVNGKYNQSLADRKKFED-QAKELALENER---LRRQLDDLRKQLEAETLARVDLENQNQSLREELAFKDQVHTQELTETRSRRQIEISEIDGRLSRQYEAKLQQSLQELRDQYEGQMRINREEIELLYDNEIQNLKAAANRAAQGSALATEEVRLMRTKIDGLNAKLQNLEDTNAGLNARIRELENLLDTERQRHNQYIASLEAELQRMRDEMAHQLQEYQGLMDIKVSLDLEIAAYDKLLCGEERRLNIESPG 408 HSP 2 Score: 49.6766 bits (117), Expect = 9.086e-6 Identity = 35/120 (29.17%), Postives = 61/120 (50.83%), Query Frame = 2
Query: 1880 EAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELE-IEEDGWGLGDTIVTSLYNAEGEEKAVH 2236
E+E+ L KG++ I EAD+ G FI L N GT+ +LTG + R I G+E+ ++F + + G +TIW+ T + + W + +++ + L NA+ E+ A +
Sbjct: 461 ESEDRTLSEYSVNAAAKGDLEIIEADVEGRFIKLHNKGTEEINLTGWQLTR-IAGDEE-LAFKFSRGSKVLGGASVTIWSVDAGTAHDPPNNLVMKKKWPVANSMRSVLANADKEDVASY 578
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Match: viml (vimentin like [Source:ZFIN;Acc:ZDB-GENE-040426-1743]) HSP 1 Score: 118.627 bits (296), Expect = 8.400e-28 Identity = 119/377 (31.56%), Postives = 188/377 (49.87%), Query Frame = 2
Query: 470 TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRT---LLSDSEKSRAPLEVQVDSLEYEIDDLRKRLM--MLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
T K R EK +Q LN+RFASYIEK RFLE QNK L E+E+L+G Q +S+I ++YE E+ LR LL+ +EK+RA EV D+L +++ L+++L ML QR+D + +K RQD + ALA + LE +++SL++ L F K +HD E+ EL T + M + ++ ALRD++ +YEN + QE + Y S+ ++ + R+N K+E + + Q+Q++ + L N E + + N+ + + L+E + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ NFSS
Sbjct: 75 TEFKANRLNEKAQMQSLNDRFASYIEKVRFLEQQNKILSAELEQLRG---QRSSRIADLYEEEMRDLRRQVDLLT-TEKTRA--EVMRDNLAEDVERLKEKLQDEML-------------QREDAE-------------------------------NSMKSFRQDVDNAALARVDLERKVESLQDELAFLKNLHDEELAELQTQIQGQQVQVEMDMAKPDLTAALRDVRLQYENLASKNIQESEDWYKSKFADLTEAANRNNEALRLAKQETNEYRRQVQAITCELDALRGTNESLERQMREMEENFSMESSGFQNTIGRLEEDIGNMKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRIAAPLPNFSS 401
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Match: vim (vimentin [Source:ZFIN;Acc:ZDB-GENE-011212-3]) HSP 1 Score: 114.005 bits (284), Expect = 2.863e-26 Identity = 111/393 (28.24%), Postives = 192/393 (48.85%), Query Frame = 2
Query: 428 PLIRGDSVALQSGV-----TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLM--MLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
PL R + L G+ T K R EK ++Q LN+RFASYI+K RFLE QNK L+ E+E+L+G S++ ++YE E+ +LR + ++ +EV D+L +I+ L++RL ML QR+D + L+ RQD + +LA L LE +++SL+E + F K++HD E+ E+ + +M + ++ AL+D++ +YE + QE + Y S+ ++ + R+N K+E + + Q+Q++ + L+ N E + + ++ + + L++ + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ NFSS
Sbjct: 66 PLPRLATDTLDFGLAEAINTEFKANRTNEKAEMQHLNDRFASYIDKVRFLEQQNKILIAELEQLRGKGA---SRVGDLYEDEMRELRRQVDQLTGEKSKVEVDRDNLGEDIERLKERLQEEML-------------QREDAE-------------------------------NSLRSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEELAEMQIQMQEQHLQIDMEVAKPDLTAALKDVRQQYETLASRNLQESEDWYKSKFADLSEAAARNNETIRLAKQEANDYRRQLQALTCDLEALKGTNESLERQMRELEDSFSMEASGYQDTIGRLEDDIRNMKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRITSPFPNFSS 411
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Match: desma (desmin a [Source:ZFIN;Acc:ZDB-GENE-980526-221]) HSP 1 Score: 108.612 bits (270), Expect = 2.429e-24 Identity = 102/361 (28.25%), Postives = 178/361 (49.31%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRK-RLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
R EK +LQ LN+RFASYIEK RFLE QN L EIE+L+G ++ ++I E+YE E+ +LR + R+ +E++ D+L +DDL+K +L + E+I+ + ++ ++S F ADV+ TL R DL++ ++ L E + F ++IH+ E++EL + M + ++ ALRDI+ +YE E + Y S++ ++ + ++N K+E + + Q+QS I L+ N ++ + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R++
Sbjct: 99 RTNEKAELQHLNDRFASYIEKVRFLEQQNSALTVEIERLRG---REPTRIAELYEEEMRELRRQVEALTNQRSRVEIERDNL---VDDLQKLKLRLQEEIHQK-----EEAENNLSAFR--------------ADVD-----AATLARLDLER-----------------RIEGLHEEIAFLRKIHEEEIRELQNQMQESQVQIQMDMSKPDLTAALRDIRLQYEAIAAKNISEAEDWYKSKVSDLNQAVNKNNEALREAKQETMQFRHQLQSYTCEIDSLKGTNESLRRQMSEMEERLGREAGGYQDTIARLEAEIAKMKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRISL 412
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Match: desma (desmin a [Source:ZFIN;Acc:ZDB-GENE-980526-221]) HSP 1 Score: 108.227 bits (269), Expect = 2.734e-24 Identity = 102/361 (28.25%), Postives = 178/361 (49.31%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRK-RLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
R EK +LQ LN+RFASYIEK RFLE QN L EIE+L+G ++ ++I E+YE E+ +LR + R+ +E++ D+L +DDL+K +L + E+I+ + ++ ++S F ADV+ TL R DL++ ++ L E + F ++IH+ E++EL + M + ++ ALRDI+ +YE E + Y S++ ++ + ++N K+E + + Q+QS I L+ N ++ + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R++
Sbjct: 99 RTNEKAELQHLNDRFASYIEKVRFLEQQNSALTVEIERLRG---REPTRIAELYEEEMRELRRQVEALTNQRSRVEIERDNL---VDDLQKLKLRLQEEIHQK-----EEAENNLSAFR--------------ADVD-----AATLARLDLER-----------------RIEGLHEEIAFLRKIHEEEIRELQNQMQESQVQIQMDMSKPDLTAALRDIRLQYEAIAAKNISEAEDWYKSKVSDLNQAVNKNNEALREAKQETMQFRHQLQSYTCEIDSLKGTNESLRRQMSEMEERLGREAGGYQDTIARLEAEIAKMKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRISL 412
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Match: lmnb2 (lamin B2 [Source:ZFIN;Acc:ZDB-GENE-990630-13]) HSP 1 Score: 104.76 bits (260), Expect = 7.720e-23 Identity = 105/402 (26.12%), Postives = 196/402 (48.76%), Query Frame = 2
Query: 452 ALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEID-----------DLRKRLMMLEDINSQY---KSTIDKQRKDISGFEGELVELRR--FKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEM--------GRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNT 1582
A Q+ ++ + R QEK +L+ LN+R A YI++ R LE +N +L ++ + + T ++ S IK +YE E+ R +L ++ + RA L++++ + E++ DL L+ ++D+ +QY ++ ++ + + EL +L+ K V K++ EV+TL R DL+ QSL E LEF K + D EV+E R ++ MV S M +AL+D++ ++E ++ K+EL ++ ++L + S ++ +EE +I+ + +++ L+ Q + E + + + QL + ++ ++ + L E Q LLD KL L++EI AY KLLE EE+R+ S ++
Sbjct: 11 AAQTPLSPARISRLQEKEELRALNDRLAVYIDRVRALELENDRLQFKVSEKEEVTTREVSGIKSLYEAELADARRVLDETARERAKLQIELGKVNSELEEVTRNYKKKDGDLAAALVRIKDLEAQYNKNEAALNTALSENASLSAELADLKAQLAKAEDAHGVAKKQLEVETLMRVDLEN-----------------RCQSLTEELEFRKSMFDEEVRE-----TRRRHEKRMVEVDSGMQQDYEFKLSQALQDLRKQHEEQVQIYKEELQHTFKAKLDNAKVSSDLNDKAVLTAREELQEAHVRIEGLSYQLSALQKQASAAEERIRELEGLLSSDRDKYRRQLDAKEREMAEMRECMQQQLNEYQELLDVKLALDMEINAYRKLLEGEEDRLKLSPSPSS 390 HSP 2 Score: 55.4546 bits (132), Expect = 2.368e-7 Identity = 30/103 (29.13%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 1931 GNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI---EEDGWGLGDTIVTSLYNAEGEEKA 2230
G++SI E D+ G + L NN K +SL +KR I G+ + + + + L +G+ +T+W+ ++ + WG G+ I+T L N+ GE+ A
Sbjct: 438 GSVSIEEIDLEGKSVTLRNNSDKDQSLGSWRLKRQI-GDGEEIVYKFSPKFVLKAGQTVTVWSADAGMSHSPPSDLLWKSQSSWGTGENILTLLVNSSGEDVA 539
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Match: vim (vimentin [Source:NCBI gene;Acc:496727]) HSP 1 Score: 122.094 bits (305), Expect = 9.821e-29 Identity = 107/361 (29.64%), Postives = 189/361 (52.35%), Query Frame = 2
Query: 479 KKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV 1558
K R EK ++Q+LN+RFA++I+K RFLE QNK LV E+E+LKG GT S+I ++YE E+ +LR L ++ +EV D+L ++ LR++L + E+++ KEE + +L+ RQD + +LA + LE +++SL+E + F K++HD E++EL + +M + ++ ALRD++ +YEN + + Y S+ ++ + R+N K++ + + QIQ++ I ++ N YE + + N+ + + + LQE++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 97 KANRTNEKAEMQELNDRFANFIDKVRFLEQQNKILVAELEQLKGKGT----SRIGDLYEEEMRELRRQLDQVTNAKDRVEVDRDNLADDLQRLREKL------------------------QDEMIQ---------------KEEAEG---NLQSFRQDVDNASLARIDLERKVESLQEEIVFLKKLHDEEIRELQLQIQESHIQVDMDVSKPDLTAALRDVRQQYENVAAKNLSDAEDWYKSKFADLSEAANRNNDALRQAKQDANDYRRQIQTLTCEIDAMKGSNESYERQMREMEENFAGEAANYQDTIQRLQEEIHNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRI 411
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Match: vim (vimentin [Source:NCBI gene;Acc:496727]) HSP 1 Score: 121.709 bits (304), Expect = 1.124e-28 Identity = 107/361 (29.64%), Postives = 189/361 (52.35%), Query Frame = 2
Query: 479 KKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV 1558
K R EK ++Q+LN+RFA++I+K RFLE QNK LV E+E+LKG GT S+I ++YE E+ +LR L ++ +EV D+L ++ LR++L + E+++ KEE + +L+ RQD + +LA + LE +++SL+E + F K++HD E++EL + +M + ++ ALRD++ +YEN + + Y S+ ++ + R+N K++ + + QIQ++ I ++ N YE + + N+ + + + LQE++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 99 KANRTNEKAEMQELNDRFANFIDKVRFLEQQNKILVAELEQLKGKGT----SRIGDLYEEEMRELRRQLDQVTNAKDRVEVDRDNLADDLQRLREKL------------------------QDEMIQ---------------KEEAEG---NLQSFRQDVDNASLARIDLERKVESLQEEIVFLKKLHDEEIRELQLQIQESHIQVDMDVSKPDLTAALRDVRQQYENVAAKNLSDAEDWYKSKFADLSEAANRNNDALRQAKQDANDYRRQIQTLTCEIDAMKGSNESYERQMREMEENFAGEAANYQDTIQRLQEEIHNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRI 413
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Match: vim (vimentin [Source:NCBI gene;Acc:496727]) HSP 1 Score: 121.709 bits (304), Expect = 1.171e-28 Identity = 110/370 (29.73%), Postives = 193/370 (52.16%), Query Frame = 2
Query: 479 KKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV-----NFSS 1570
K R EK ++Q+LN+RFA++I+K RFLE QNK LV E+E+LKG GT S+I ++YE E+ +LR L ++ +EV D+L ++ LR++L + E+++ KEE + +L+ RQD + +LA + LE +++SL+E + F K++HD E++EL + +M + ++ ALRD++ +YEN + + Y S+ ++ + R+N K++ + + QIQ++ I ++ N YE + + N+ + + + LQE++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ +FSS
Sbjct: 93 KANRTNEKAEMQELNDRFANFIDKVRFLEQQNKILVAELEQLKGKGT----SRIGDLYEEEMRELRRQLDQVTNAKDRVEVDRDNLADDLQRLREKL------------------------QDEMIQ---------------KEEAEG---NLQSFRQDVDNASLARIDLERKVESLQEEIVFLKKLHDEEIRELQLQIQESHIQVDMDVSKPDLTAALRDVRQQYENVAAKNLSDAEDWYKSKFADLSEAANRNNDALRQAKQDANDYRRQIQTLTCEIDAMKGSNESYERQMREMEENFAGEAANYQDTIQRLQEEIHNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRITVPVHSFSS 416
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Match: rnasek (ribonuclease K [Source:Xenbase;Acc:XB-GENE-6046202]) HSP 1 Score: 107.457 bits (267), Expect = 1.560e-23 Identity = 109/395 (27.59%), Postives = 208/395 (52.66%), Query Frame = 2
Query: 428 PLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQL---VNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLR----KRLMMLEDINSQYKST---IDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKEL-----ATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
P G ++ + ++ + R QEK DLQ+LN+R A YI+K R LE++N L V E E+++ V S IKE+YE E+ R L D+ + RA L++++ + E +DLR K+ LE ++++ T ++ + ++ + E K QG +V K E+ L L ++ E+E L + LE+ QSL E L+F K +++ E+KE + D K+ + ++ ++ +AL D++ ++++++T K+EL+ +Y S+L+ + S ++ +EE + +I S+ S++++L+ ++ + + + + + + L + ++ + + L + + LLD KL L++EI+AY KLLE EE R+ S
Sbjct: 9 PRSSGRRSSMNTPLSPTRISRLQEKVDLQELNDRLAVYIDKVRSLESENSLLHVQVTEREEVRSREV---SGIKELYETELADARRSLDDTARERARLQLELSKISVEHEDLRASFSKKESELESAQARFRETEALLNSKNAALATTQSE------NKSLQG-EVEDLKAEIGQLESALALAKKQLEEEILIKVDLENRCQSLIEELDFRKNVYEEEIKETRRRHETRLVEVDSGKQ--IEYEHKLSQALADMREQHDSQVTLYKEELEHTYQSKLENARLSSEMNSSAVNSTREELMESRYRIDSLTSQLSELQKESRAWHDRMQELEDMLAKEKDNSRKMLAEREREMADIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLS 391
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Match: rnasek (ribonuclease K [Source:Xenbase;Acc:XB-GENE-6046202]) HSP 1 Score: 106.686 bits (265), Expect = 2.875e-23 Identity = 108/391 (27.62%), Postives = 207/391 (52.94%), Query Frame = 2
Query: 440 GDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQL---VNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLR----KRLMMLEDINSQYKST---IDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKEL-----ATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
G ++ + ++ + R QEK DLQ+LN+R A YI+K R LE++N L V E E+++ V S IKE+YE E+ R L D+ + RA L++++ + E +DLR K+ LE ++++ T ++ + ++ + E K QG +V K E+ L L ++ E+E L + LE+ QSL E L+F K +++ E+KE + D K+ + ++ ++ +AL D++ ++++++T K+EL+ +Y S+L+ + S ++ +EE + +I S+ S++++L+ ++ + + + + + + L + ++ + + L + + LLD KL L++EI+AY KLLE EE R+ S
Sbjct: 21 GRRSSMNTPLSPTRISRLQEKVDLQELNDRLAVYIDKVRSLESENSLLHVQVTEREEVRSREV---SGIKELYETELADARRSLDDTARERARLQLELSKISVEHEDLRASFSKKESELESAQARFRETEALLNSKNAALATTQSE------NKSLQG-EVEDLKAEIGQLESALALAKKQLEEEILIKVDLENRCQSLIEELDFRKNVYEEEIKETRRRHETRLVEVDSGKQ--IEYEHKLSQALADMREQHDSQVTLYKEELEHTYQSKLENARLSSEMNSSAVNSTREELMESRYRIDSLTSQLSELQKESRAWHDRMQELEDMLAKEKDNSRKMLAEREREMADIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLS 399 HSP 2 Score: 56.9954 bits (136), Expect = 1.069e-7 Identity = 31/103 (30.10%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1931 GNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI---EEDGWGLGDTIVTSLYNAEGEEKA 2230
G++SI E D++G +I L+NN + L G + R I E +V+F +R L +G+ +TIWA ++ ++ W G+ + L N++GE+ A
Sbjct: 448 GDVSIEEVDVDGKYIRLKNNSEQDHPLGGWELTRTI--GEASVNFKFTSRYVLKAGQTVTIWAADAGVTASPPSDLIWKNQNSWATGEDVKVILKNSQGEDVA 548
BLAST of Intermediate filament protein vs. Ensembl Mouse
Match: Vim (vimentin [Source:MGI Symbol;Acc:MGI:98932]) HSP 1 Score: 122.479 bits (306), Expect = 3.468e-29 Identity = 113/387 (29.20%), Postives = 203/387 (52.45%), Query Frame = 2
Query: 422 NIPLIR--GDSV--ALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
++P +R DSV +L + T K R EK +LQ+LN+RFA+YI+K RFLE QNK L+ E+E+LKG Q S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L + E+++ +EE ++ L+ RQD + +LA L LE +++SL+E + F K++HD E++EL + ++ + ++ ALRD++ +YE+ QE + Y S+ ++ + R+N K+E++ + Q+QS+ + L+ N E + + N+ + + + LQ+++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R++
Sbjct: 73 SVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG---QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKL------------------------QEEMLQ---------------REEAEST---LQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLP 414
BLAST of Intermediate filament protein vs. Ensembl Mouse
Match: Vim (vimentin [Source:MGI Symbol;Acc:MGI:98932]) HSP 1 Score: 121.709 bits (304), Expect = 8.803e-29 Identity = 113/387 (29.20%), Postives = 203/387 (52.45%), Query Frame = 2
Query: 422 NIPLIR--GDSV--ALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
++P +R DSV +L + T K R EK +LQ+LN+RFA+YI+K RFLE QNK L+ E+E+LKG Q S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L + E+++ +EE ++ L+ RQD + +LA L LE +++SL+E + F K++HD E++EL + ++ + ++ ALRD++ +YE+ QE + Y S+ ++ + R+N K+E++ + Q+QS+ + L+ N E + + N+ + + + LQ+++ ++++ L E Q LL+ K+ L++EIA Y KLLE EE+R++
Sbjct: 73 SVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG---QGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKL------------------------QEEMLQ---------------REEAEST---LQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLP 414
BLAST of Intermediate filament protein vs. Ensembl Mouse
Match: Gfap (glial fibrillary acidic protein [Source:MGI Symbol;Acc:MGI:95697]) HSP 1 Score: 107.457 bits (267), Expect = 2.780e-24 Identity = 100/372 (26.88%), Postives = 180/372 (48.39%), Query Frame = 2
Query: 452 ALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
AL +G K+ RA E+ ++ +LN+RFASYIEK RFLE QNK L E+ +L+ ++ +K+ ++Y+ E+ +LR L + A LEV+ D+ ++ LR++L ++ + ++ + A +E +E R DL++ +++SL+E ++F ++I++ EV+EL + M + ++ ALR+I+ +YE T QE + Y S+ ++ + +R+ K E + + Q+Q++ + L N E + + S + L L+E+ ++++ L E Q LL+ KL L++EIA Y KLLE EENR+
Sbjct: 54 ALNAG---FKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRA---KEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQDETNLRLEAENNL-------------------------AAYRQEADEATLARVDLER-----------------KVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIP 377
BLAST of Intermediate filament protein vs. Ensembl Mouse
Match: Gfap (glial fibrillary acidic protein [Source:MGI Symbol;Acc:MGI:95697]) HSP 1 Score: 107.071 bits (266), Expect = 3.992e-24 Identity = 103/372 (27.69%), Postives = 182/372 (48.92%), Query Frame = 2
Query: 452 ALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
AL +G K+ RA E+ ++ +LN+RFASYIEK RFLE QNK L E+ +L+ ++ +K+ ++Y+ E+ +LR L + A LEV+ D+ ++ LR++L ++ N + ++ E L R +E +E R DL++ +++SL+E ++F ++I++ EV+EL + M + ++ ALR+I+ +YE T QE + Y S+ ++ + +R+ K E + + Q+Q++ + L N E + + S + L L+E+ ++++ L E Q LL+ KL L++EIA Y KLLE EENR+
Sbjct: 54 ALNAG---FKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRA---KEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQ--DETNLRLEA------------ENNLAAYR-----------QEADEATLARVDLER-----------------KVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIP 377
BLAST of Intermediate filament protein vs. Ensembl Mouse
Match: Des (desmin [Source:MGI Symbol;Acc:MGI:94885]) HSP 1 Score: 104.375 bits (259), Expect = 5.651e-23 Identity = 104/360 (28.89%), Postives = 175/360 (48.61%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
R EK +LQ+LN+RFA+YIEK RFLE QN L E+ +LKG ++ +++ E+YE E+ +LR + RA ++V+ D+L IDDL++ L+ E ++LR EE + ++L R D + LA + LE ++SL E + F K++H+ E++EL + M + ++ ALRDI+ +YE E + Y S++ ++ + ++N K+E + QIQS I L+ N + + + + + L+E++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+N
Sbjct: 104 RTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVNRLKG---REPTRVAELYEEEMRELRRQVEVLTNQRARVDVERDNL---IDDLQRLKAKLQ----------------------EEIQLR--------------EEAE---NNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEANGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLP 418
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Match: sp|Q01240|NF60_DORPE (60 kDa neurofilament protein OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1) HSP 1 Score: 237.269 bits (604), Expect = 4.614e-68 Identity = 150/371 (40.43%), Postives = 238/371 (64.15%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQY---KSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
+GV +K R +EK+D++DLNERFA+YIEK RFLEAQNK+L E+E+LK ++ S IKE+YE E+ + R L+ + K + L+V+V L ID L ++ LE+ + + + I +Q + ++ EGE+ LRR + + ++ + + D+++K+R D E + HL E+ Q+L+E LEF+K +H E+KELA +AYRD T EN +W++E+ +A+RDIQ EY+ K ++ ++++ Y ++QE +T T+ N+E T KEEN+++KS + +R+R+ADLE +NA+ E ++ + EE R EL+ +E++T + ++ L ELQ L+D KL LELEIAAY KLLE EE+RV
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIKEMYETELEEARKLIDATNKEKITLDVRVTEL---IDQLERQQKDLEESRTYHQIDQEQIARQNQQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAENRRQTLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNELAQAIRDIQQEYDAKCDQMRGDIEAYYNLKVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLLEGEESRVGM 451
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Match: sp|Q01241|NF70_DORPE (70 kDa neurofilament protein OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1) HSP 1 Score: 236.884 bits (603), Expect = 5.077e-67 Identity = 150/371 (40.43%), Postives = 238/371 (64.15%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQY---KSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
+GV +K R +EK+D++DLNERFA+YIEK RFLEAQNK+L E+E+LK ++ S IKE+YE E+ + R L+ + K + L+V+V L ID L ++ LE+ + + + I +Q + ++ EGE+ LRR + + ++ + + D+++K+R D E + HL E+ Q+L+E LEF+K +H E+KELA +AYRD T EN +W++E+ +A+RDIQ EY+ K ++ ++++ Y ++QE +T T+ N+E T KEEN+++KS + +R+R+ADLE +NA+ E ++ + EE R EL+ +E++T + ++ L ELQ L+D KL LELEIAAY KLLE EE+RV
Sbjct: 84 TGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSAIKEMYETELEEARKLIDATNKEKITLDVRVTEL---IDQLERQQKDLEESRTYHQIDQEQIARQNQQLADLEGEISMLRRSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAENRRQTLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNELAQAIRDIQQEYDAKCDQMRGDIEAYYNLKVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLLEGEESRVGM 451 HSP 2 Score: 68.9366 bits (167), Expect = 1.109e-10 Identity = 44/112 (39.29%), Postives = 64/112 (57.14%), Query Frame = 2
Query: 1913 FRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXE--LEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
++RT+KG++SI EAD G FI LE K E+LTG I R +D N+ ++ I N L +G + IW+K + + + E D WG G +VT L N +GE+KA + Q
Sbjct: 503 YQRTSKGSVSIKEADSQGCFIALETK--KEENLTGWKIVRKVDDNK-VYTYEIPNLV-LKTGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Match: sp|P90901|IFA1_CAEEL (Intermediate filament protein ifa-1 OS=Caenorhabditis elegans OX=6239 GN=ifa-1 PE=1 SV=2) HSP 1 Score: 188.348 bits (477), Expect = 6.574e-50 Identity = 125/369 (33.88%), Postives = 221/369 (59.89%), Query Frame = 2
Query: 458 QSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
Q+ ++++ R +EK+++ DLN+R ASYIEK RFLEAQN++L +++ L+ +D I+ +YE E+ + L+ ++ K R +E Q+ ++ E+ ++R++L Q ++ ID +S E E+ L+R +V + K+E + L +L++ R D ++E L + ++++Q+L E ++F +++HD E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +++S Y ++QEIQT R N+E + KEE R+++Q+ +R ++ADLE +N+ E + + E+ QRS E L + Q+ +++ + ELQ LLD K L+ EIA Y K+LE EENR
Sbjct: 56 QNAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIRNMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALLVTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENRAGL 424 HSP 2 Score: 73.1738 bits (178), Expect = 4.796e-12 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 2
Query: 1904 RMKFRRTTKGNISIAEADINGLFIVLEN-NGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI--EEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
R F+R+ KGN+SI EA +G FIVL+N + K E++ +KR IDG + V +T+ L +G+ L I+A+ + + + ED +G G+ + T L+N EGEE+A H Q
Sbjct: 456 RQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRENV-YTLPRDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Match: sp|P90900|IFA4_CAEEL (Intermediate filament protein ifa-4 OS=Caenorhabditis elegans OX=6239 GN=ifa-4 PE=1 SV=3) HSP 1 Score: 174.866 bits (442), Expect = 2.972e-45 Identity = 117/367 (31.88%), Postives = 209/367 (56.95%), Query Frame = 2
Query: 464 GVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
+++++ R +EK+++ +LN+R ASYIEK RFLEAQN+++ ++ L+G D++ +K +YE E+ + L++DS+K RA LE Q+ L E+++ R +L E + +D + E E+ L+R + K E + D L R E+E L + ++++Q+L E +F ++IHD+E+ +L +A RD T EN Y+++E+ A+R+I++EY++ + +++S Y ++QEI T +R+ +E + +EE R+++ + MR ++ADLE +N E + ++ EE RS E L + + + + I + ELQ L+D K L+ EI Y K+L+ EENR
Sbjct: 59 AASAIRESRTREKKEMSELNDRLASYIEKVRFLEAQNRKMEKDLNLLRGKWGHDSTSVKVMYETELRSAKDLIADSDKERAQLEDQIKKLVEELNNYRNKLHEAERGADVTRKELDNVLGRLGALEAEIELLKRRISLMEESIGHLKRENHRMIDSLHHARNAVEQETLNRIDYQNQVQTLLEECDFIRRIHDSEIHDLLAMASRDTTVENREYFKNELSSAIREIRSEYDHVSNTTRTDIESWYKLKVQEIHTQNSRNCLEQNYAREEVKRLRTTLGDMRGKMADLEGRNLLLEKQIEDLNYQMEEDMRSYEQSLNDKDTSINKLRDESKILMVELQMLIDTKQTLDAEIVIYRKMLDGEENRAGL 425 HSP 2 Score: 67.0106 bits (162), Expect = 4.353e-10 Identity = 40/112 (35.71%), Postives = 67/112 (59.82%), Query Frame = 2
Query: 1913 FRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIE--EDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
+ R+ KGNI+I EA+ +G +IVLEN + E++ ++R I G + V FT +L + + + I+A+G+ + + ED +G G+ +VT+LYN EGE++A H Q
Sbjct: 460 YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKREIV-FTFPREFTLRAQKSVKIFARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQ 570
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Match: sp|Q6QUW1|RGP51_LYMST (Retrograde protein of 51 kDa OS=Lymnaea stagnalis OX=6523 GN=RGP51 PE=1 SV=1) HSP 1 Score: 165.236 bits (417), Expect = 9.673e-43 Identity = 124/383 (32.38%), Postives = 205/383 (53.52%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHD------TEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQS-------MRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSS 1570
SG+T K R +EKR++Q+LNER ASYIEK FL+AQ K+L E E L+ V+D I++ YE E+ Q R ++ + S+ E ++ L+ EI LR+ ++ E Y+ ID + FEGEL LR G+ + K +E ++ +++ ++LR D + E AH+ + Q+ E EF + + D E ++ + Y D +W+SE+ + +R+I Y+ K+ I+Q+ ++ Y SQ+ ++++ +D ++ H +EE +++ Q+Q + +RIA L+ + E L +I EE Q L + + +LT L +LQ L+DAK+ LELEIA Y KLLE EE+RV +
Sbjct: 60 SGITDFKGNREKEKREMQNLNERLASYIEKVHFLDAQVKKLEAENEALRNRKVEDLQPIRDAYENELRQARKVIDELASSKGVAEGKLAGLQDEIGSLRELIVTYESQAKDYRKKIDSLGNQLGEFEGELQSLRLRVGSLEDENAKLRELLEKVQEQNRRLRADLDTETAAHIEADCLAQTKTEEAEFYRDLLDQLELLKPEPIQIKGMDYAD-------FWKSELAKCVREINLAYDEKIDLIQQDCEAKYASQINQLRSGNVKDGMQLQHSQEEVKKLRGQLQDKNAAYAELATRIASLQAERDELARQLADIERELEE-------QKLKYNRDVGDLESELTSVLAQLQHLMDAKMSLELEIACYKKLLEGEESRVGLRT 428
BLAST of Intermediate filament protein vs. TrEMBL
Match: A0A5J6BTN2 (Intermediate filament b-like protein 1 (Fragment) OS=Minona ileanae OX=60013 PE=2 SV=1) HSP 1 Score: 289.656 bits (740), Expect = 4.666e-84 Identity = 173/377 (45.89%), Postives = 261/377 (69.23%), Query Frame = 2
Query: 434 IRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
+ G + + +GV+++ + R +EK+D++DLNERFA+YIEK RFLEAQNK+L NE+ L+ ++ +IK++Y+VE+ QLR LL D+ KS+A LEV+V+SLE +D+L+K+L +++ + ID+ +S E EL LRR + +D +++K EV+ LRD++++LR D ++E L H+ E E SL++ +EF K++HD+E+KELA +AYRD T+EN +W+SEM + LRDIQNEY+NKL ++ E+++ Y ++QE +T+ TR E H KEEN R+K Q+ +R RI +LE +NA+ E L +R + EE R EL+ +L++ L+ Q +L L ELQ+LLDAKL LELEIAAY KLLE EE+R
Sbjct: 76 VNGHTAGVHNGVSNIMENREKEKKDMRDLNERFANYIEKVRFLEAQNKKLANELATLRDRWGKETERIKQMYDVEMTQLRKLLDDAMKSKAELEVKVNSLEETVDELQKQLDAANQLHNSDREMIDRLNSQVSDQESELSVLRRRISSFESDHSRDKAEVQRLRDEIRRLRMDLDQETLNHINSEQEAHSLRDEIEFLKKVHDSELKELAALAYRDTTQENREFWRSEMSQCLRDIQNEYDNKLDVMQSEMETHYNLKIQESRTNNTRGGAELQHTKEENIRIKQQLSELRDRIPELEARNAQLERLLEELRRDNEEKLRDWELERSSLRKTLSDNQAELESVLVELQTLLDAKLSLELEIAAYRKLLEGEESRAGL 452 HSP 2 Score: 88.5817 bits (218), Expect = 2.373e-14 Identity = 45/113 (39.82%), Postives = 66/113 (58.41%), Query Frame = 2
Query: 1913 FRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSS---GEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
++RT KGNISI E +G F+ +EN G K E ++GM I RN+D + FT ++T L + + +WA+G K ++E E+ WG G I T L +++GEEKA H Q
Sbjct: 498 YQRTAKGNISIQECAPDGKFLTIENTGKKREDISGMRITRNVDNGRQIIRFTFPDKTFLEPEGRAKSIKVWARGLKPPGSSDMECNENTWGNGANIHTVLISSDGEEKATHTQ 610
BLAST of Intermediate filament protein vs. TrEMBL
Match: A0A1I8JCN5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 282.722 bits (722), Expect = 2.902e-81 Identity = 165/367 (44.96%), Postives = 253/367 (68.94%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
+G+ +M GR +EK+D+++LNERFASYIEK RFLEAQN++L E++KL+ ++ K++ +YE E+ QLR LL D+++ +A +EV+V +LE +++++ + L +++ + TIDK + ++ ++GE+ LRR + + +++ E+K LR D+ +LR D + E L H+ E+ QSL+E LEF + +HD+E+KEL+ +AYRD T EN +W++EM +AL +IQ EY++KL ++ E++S Y ++QE +TS TRDN+E TH KEEN R+KSQI R+RI +LE +NA+ E + ++R EET RS EL+ LQ L+ L L ELQ+L+DAKL LELEI AY KLLE EE R+N
Sbjct: 95 AGLNNMLTGREKEKKDMRELNERFASYIEKVRFLEAQNRKLSEELQKLRDSWGKETEKVRSLYETELAQLRKLLEDADQYKATMEVRVTTLEDQLNEMEQLLEEANRVHANDRDTIDKLNQQMADYDGEISLLRRRVASFDDERARDQAEIKRLRQDVARLRGDLDTETLNHINAENSAQSLREELEFLRALHDSELKELSALAYRDTTSENREFWKNEMAQALHEIQREYDSKLDVVRSEMESQYSIKVQEARTSNTRDNMELTHLKEENRRIKSQIGDARNRIPELEARNAQLEREIEDLRRELEETMRSSELEKNQLQADLSSCNATLAEYLRELQTLMDAKLSLELEIHAYRKLLEGEEIRIN 461 HSP 2 Score: 91.2781 bits (225), Expect = 3.665e-15 Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2
Query: 1919 RTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEK-LTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
RT+KG+I+I E D G F+ LE G K E L+ T+ RN+D + V +T T + SG K + IWAKG++ K+LE+ + WG+G IVT L NA+GEEKA H Q
Sbjct: 522 RTSKGSITIQECDSEGKFVTLEFTGKKKEDLSDWTLVRNVDNEKLIVKYTFPKDTVMDSGNKTIKIWAKGQRPADSKDLEMNDQSWGMGSHIVTMLSNADGEEKATHTQ 630
BLAST of Intermediate filament protein vs. TrEMBL
Match: A0A267DRI3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002432g2 PE=3 SV=1) HSP 1 Score: 283.108 bits (723), Expect = 3.027e-81 Identity = 165/367 (44.96%), Postives = 253/367 (68.94%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
+G+ +M GR +EK+D+++LNERFASYIEK RFLEAQN++L E++KL+ ++ K++ +YE E+ QLR LL D+++ +A +EV+V +LE +++++ + L +++ + TIDK + ++ ++GE+ LRR + + +++ E+K LR D+ +LR D + E L H+ E+ QSL+E LEF + +HD+E+KEL+ +AYRD T EN +W++EM +AL +IQ EY++KL ++ E++S Y ++QE +TS TRDN+E TH KEEN R+KSQI R+RI +LE +NA+ E + ++R EET RS EL+ LQ L+ L L ELQ+L+DAKL LELEI AY KLLE EE R+N
Sbjct: 95 AGLNNMLTGREKEKKDMRELNERFASYIEKVRFLEAQNRKLSEELQKLRDSWGKETEKVRSLYETELAQLRKLLEDADQYKATMEVRVTTLEDQLNEMEQLLEEANRVHANDRDTIDKLNQQMADYDGEISLLRRRVASFDDERARDQAEIKRLRQDVARLRGDLDTETLNHINAENSAQSLREELEFLRALHDSELKELSALAYRDTTSENREFWKNEMAQALHEIQREYDSKLDVVRSEMESQYSIKVQEARTSNTRDNMELTHLKEENRRIKSQIGDARNRIPELEARNAQLEREIEDLRRELEETMRSSELEKNQLQADLSSCNATLAEYLRELQTLMDAKLSLELEIHAYRKLLEGEEIRIN 461 HSP 2 Score: 91.2781 bits (225), Expect = 3.763e-15 Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2
Query: 1919 RTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEK-LTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
RT+KG+I+I E D G F+ LE G K E L+ T+ RN+D + V +T T + SG K + IWAKG++ K+LE+ + WG+G IVT L NA+GEEKA H Q
Sbjct: 526 RTSKGSITIQECDSEGKFVTLEFTGKKKEDLSDWTLVRNVDNEKLIVKYTFPKDTVMDSGNKTIKIWAKGQRPADSKDLEMNDQSWGMGSHIVTMLSNADGEEKATHTQ 634
BLAST of Intermediate filament protein vs. TrEMBL
Match: A0A267GZY0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002432g3 PE=3 SV=1) HSP 1 Score: 282.722 bits (722), Expect = 3.525e-81 Identity = 165/367 (44.96%), Postives = 253/367 (68.94%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
+G+ +M GR +EK+D+++LNERFASYIEK RFLEAQN++L E++KL+ ++ K++ +YE E+ QLR LL D+++ +A +EV+V +LE +++++ + L +++ + TIDK + ++ ++GE+ LRR + + +++ E+K LR D+ +LR D + E L H+ E+ QSL+E LEF + +HD+E+KEL+ +AYRD T EN +W++EM +AL +IQ EY++KL ++ E++S Y ++QE +TS TRDN+E TH KEEN R+KSQI R+RI +LE +NA+ E + ++R EET RS EL+ LQ L+ L L ELQ+L+DAKL LELEI AY KLLE EE R+N
Sbjct: 95 AGLNNMLTGREKEKKDMRELNERFASYIEKVRFLEAQNRKLSEELQKLRDSWGKETEKVRSLYETELAQLRKLLEDADQYKATMEVRVTTLEDQLNEMEQLLEEANRVHANDRDTIDKLNQQMADYDGEISLLRRRVASFDDERARDQAEIKRLRQDVARLRGDLDTETLNHINAENSAQSLREELEFLRALHDSELKELSALAYRDTTSENREFWKNEMAQALHEIQREYDSKLDVVRSEMESQYSIKVQEARTSNTRDNMELTHLKEENRRIKSQIGDARNRIPELEARNAQLEREIEDLRRELEETMRSSELEKNQLQADLSSCNATLAEYLRELQTLMDAKLSLELEIHAYRKLLEGEEIRIN 461 HSP 2 Score: 90.5077 bits (223), Expect = 5.504e-15 Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2
Query: 1919 RTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEK-LTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
RT+KG+I+I E D G F+ LE G K E L+ T+ RN+D + V +T T + SG K + IWAKG++ K+LE+ + WG+G IVT L NA+GEEKA H Q
Sbjct: 525 RTSKGSITIQECDSEGKFVTLEFTGKKKEDLSDWTLVRNVDNEKLIVKYTFPKDTVMDSGNKTIKIWAKGQRPADSKDLEMNDQSWGMGSHIVTLLSNADGEEKATHTQ 633
BLAST of Intermediate filament protein vs. TrEMBL
Match: A0A267DRG7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002432g1 PE=3 SV=1) HSP 1 Score: 278.485 bits (711), Expect = 1.425e-80 Identity = 164/362 (45.30%), Postives = 249/362 (68.78%), Query Frame = 2
Query: 476 MKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
M GR +EK+D+++LNERFASYIEK RFLEAQN++L E++KL+ ++ K++ +YE E+ QLR LL D+++ +A +EV+V +LE +++++ + L +++ + TIDK + ++ ++GE+ LRR + + +++ E+K LR D+ +LR D + E L H+ E+ QSL+E LEF + +HD+E+KEL+ +AYRD T EN +W++EM +AL +IQ EY++KL ++ E++S Y ++QE +TS TRDN+E TH KEEN R+KSQI R+RI +LE +NA+ E + ++R EET RS EL+ LQ L+ L L ELQ+L+DAKL LELEI AY KLLE EE R+N
Sbjct: 1 MLTGREKEKKDMRELNERFASYIEKVRFLEAQNRKLSEELQKLRDSWGKETEKVRSLYETELAQLRKLLEDADQYKATMEVRVTTLEDQLNEMEQLLEEANRVHANDRDTIDKLNQQMADYDGEISLLRRRVASFDDERARDQAEIKRLRQDVARLRGDLDTETLNHINAENSAQSLREELEFLRALHDSELKELSALAYRDTTSENREFWKNEMAQALHEIQREYDSKLDVVRSEMESQYSIKVQEARTSNTRDNMELTHLKEENRRIKSQIGDARNRIPELEARNAQLEREIEDLRRELEETMRSSELEKNQLQADLSSCNATLAEYLRELQTLMDAKLSLELEIHAYRKLLEGEEIRIN 362 HSP 2 Score: 91.6633 bits (226), Expect = 1.850e-15 Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2
Query: 1919 RTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEK-LTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQ 2242
RT+KG+I+I E D G F+ LE G K E L+ T+ RN+D + V +T T + SG K + IWAKG++ K+LE+ + WG+G IVT L NA+GEEKA H Q
Sbjct: 427 RTSKGSITIQECDSEGKFVTLEFTGKKKEDLSDWTLVRNVDNEKLIVKYTFPKDTVMDSGNKTIKIWAKGQRPADSKDLEMNDQSWGMGSHIVTMLSNADGEEKATHTQ 535
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Match: vim (vimentin [Source:NCBI gene;Acc:103044141]) HSP 1 Score: 114.775 bits (286), Expect = 1.194e-26 Identity = 113/382 (29.58%), Postives = 190/382 (49.74%), Query Frame = 2
Query: 428 PLIRGDSVALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
P R D AL + T K R EK ++Q LN+RFASYIEK RFLE QNK L E+E L+G GT S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L E+I QR+D ++L RQD + +LA L LE +L+SL+E + F K++HD E+ EL + + +M + ++ ALRD++++YE T QE + Y S+ ++ + R + K++ + + Q+ ++ + L+ N E + + ++ + ++ L++ + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ F
Sbjct: 65 PSPRLD-FALSDAINTEFKANRTNEKAEMQHLNDRFASYIEKVRFLEQQNKSLSAELELLRGKGT----SRVGDLYEEEMRELRLQVDQLIGDKARVEVERDNLGEDIQRLREKLQ--EEI---------IQREDAE-------------------------------NNLHGFRQDVDNASLARLDLERKLESLQEEIIFLKKLHDEEIAELQAQIHDQHVQIDMDVAKPDLTAALRDVRSQYETLATKNIQEAEDWYKSKFTDLSDAAARSSEAIRLAKQDANEYRRQVHALTCEVDALKGTNESLERQMRELEDSFGMESSGYQDTIVRLEDDIRNMKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRLCFP 399
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Match: vim (vimentin [Source:NCBI gene;Acc:103044141]) HSP 1 Score: 112.849 bits (281), Expect = 5.421e-26 Identity = 112/379 (29.55%), Postives = 189/379 (49.87%), Query Frame = 2
Query: 428 PLIRGDSVALQSGV-TSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV 1558
P R D AL + T K R EK ++Q LN+RFASYIEK RFLE QNK L E+E L+G GT S++ ++YE E+ +LR + +A +EV+ D+L +I LR++L E+I QR+D ++L RQD + +LA L LE +L+SL+E + F K++HD E+ EL + + +M + ++ ALRD++++YE T QE + Y S+ ++ + R + K++ + + Q+ ++ + L+ N E + + ++ + ++ L++ + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 65 PSPRLD-FALSDAINTEFKANRTNEKAEMQHLNDRFASYIEKVRFLEQQNKSLSAELELLRGKGT----SRVGDLYEEEMRELRLQVDQLIGDKARVEVERDNLGEDIQRLREKLQ--EEI---------IQREDAE-------------------------------NNLHGFRQDVDNASLARLDLERKLESLQEEIIFLKKLHDEEIAELQAQIHDQHVQIDMDVAKPDLTAALRDVRSQYETLATKNIQEAEDWYKSKFTDLSDAAARSSEAIRLAKQDANEYRRQVHALTCEVDALKGTNESLERQMRELEDSFGMESSGYQDTIVRLEDDIRNMKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRI 396
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Match: desmb (desmin [Source:NCBI gene;Acc:103039642]) HSP 1 Score: 110.923 bits (276), Expect = 2.555e-25 Identity = 106/361 (29.36%), Postives = 177/361 (49.03%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
R EK +LQ LN+RFASYIEK RFLE QN+ LV E+E+L+G ++ ++I ++YE E+ +LR + R+ +EV+ D+L DDL+K M L++ +Q + E L R ADV+ TL R DL++ +++L+E + F K+IH+ E++EL + M + ++ ALRDI+++YE E + Y S++ ++ + T++N K E + QIQS I L+ N + ++ + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 108 RTNEKAELQHLNDRFASYIEKVRFLEQQNQALVVEVERLRG---REPTRIADLYEEEMRELRRQVEVVTNQRSRVEVERDNL---ADDLQKLKMRLQEEIAQREEA-----------ENSLAAFR-------ADVD-----AATLARLDLER-----------------RIETLQEEIAFLKKIHEEEIRELQAQMQETQVQIQMDMSKPDLTGALRDIRSQYEAIAAKNIAEAEDWYKSKVSDLNQAVTKNNEALKQAKLETMEFRHQIQSYTCEIDSLKGTNESLMRQMRDMEDRHGREASGFQDTITRLEAEIANMKDEMARHLREYQDLLNIKMALDVEIATYRKLLEGEESRITLP 422
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Match: desmb (desmin [Source:NCBI gene;Acc:103039642]) HSP 1 Score: 110.153 bits (274), Expect = 3.713e-25 Identity = 106/361 (29.36%), Postives = 177/361 (49.03%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
R EK +LQ LN+RFASYIEK RFLE QN+ LV E+E+L+G ++ ++I ++YE E+ +LR + R+ +EV+ D+L DDL+K M L++ +Q + E L R ADV+ TL R DL++ +++L+E + F K+IH+ E++EL + M + ++ ALRDI+++YE E + Y S++ ++ + T++N K E + QIQS I L+ N + ++ + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 85 RTNEKAELQHLNDRFASYIEKVRFLEQQNQALVVEVERLRG---REPTRIADLYEEEMRELRRQVEVVTNQRSRVEVERDNL---ADDLQKLKMRLQEEIAQREEA-----------ENSLAAFR-------ADVD-----AATLARLDLER-----------------RIETLQEEIAFLKKIHEEEIRELQAQMQETQVQIQMDMSKPDLTGALRDIRSQYEAIAAKNIAEAEDWYKSKVSDLNQAVTKNNEALKQAKLETMEFRHQIQSYTCEIDSLKGTNESLMRQMRDMEDRHGREASGFQDTITRLEAEIANMKDEMARHLREYQDLLNIKMALDVEIATYRKLLEGEESRITLP 399
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Match: desma (desmin-like [Source:NCBI gene;Acc:103037132]) HSP 1 Score: 108.997 bits (271), Expect = 1.227e-24 Identity = 106/368 (28.80%), Postives = 179/368 (48.64%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRK-RLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTS 1585
R EK +LQ LN+RFASYIEK RFLE QN LV E+++++G ++ ++I E+YE E+ +LR + RA +EV+ D+L DDL+K +L + E+I+ + ++ ++S F ADV+ TL R DL++ ++SL+E + F K+IH+ E++EL + M + ++ ALRDI+ +YE E + Y S++ ++ + ++N K E + QIQS I L+ N + + + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+ +S
Sbjct: 99 RTNEKAELQHLNDRFASYIEKVRFLEQQNAALVVELDRMRG---REPTRITEMYEEEMRELRRQVDAVTNQRARVEVERDNL---ADDLQKLKLRLQEEIHQK-----EEAENNLSAFR--------------ADVD-----AATLARLDLER-----------------RIESLQEEIAFLKKIHEEEIRELQNQMQETQVQVQMDMSKPDLTAALRDIRMQYEAIAAKNISEAEEWYKSKVSDLNQAVNKNNDALRQAKMETMEFRHQIQSYTCEIDSLKGTNESLKRQMREMEERMGGEAGGYQDTISRLEGEIAKMKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRITLPPQSFSS 419
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Match: zgc:136930 (zgc:136930 [Source:ZFIN;Acc:ZDB-GENE-060312-16]) HSP 1 Score: 82.8037 bits (203), Expect = 8.746e-17 Identity = 98/374 (26.20%), Postives = 165/374 (44.12%), Query Frame = 2
Query: 464 GVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGT-VQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQ--TSKTRDNV-----ETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
G R EK LQ LN+RF Y+EK R L+ +N L ++ + GG + + + YEV+I +LRT + + LE+++D++ ++L+ + + Q +S I +KDI E R T L D+L L++ YE +E F+ ++ TEV ++ D K ++ L I++EYE T + E ++ Y S+++EIQ TSKT + + E ++ S++Q++ S+ +E A YE ++ A+Y+ + +L+ + + DL L QSLLD KL L+ EI+ Y LLE E R +
Sbjct: 175 GTIPPMASREAEKSTLQFLNDRFGGYVEKVRLLQRENAALEAQLASITGGAPLSPDGGVTVNYEVQITELRTTIETLILDKVNLEIELDNIRATAEELKAKFEFEAGVRYQLESDISLMKKDI-----EFATDTRVNLTT---------RYTALTDELTFLKKTYE----------------EEFATFQTRLGTTEVSS-GSIIEVDTIK------SFDLMSTLSQIRSEYEVVATRNRAEAEAYYHSRIEEIQAVTSKTTEAINFAKTEIVSVTKDIQMHYSELQTLLSQSYSMEQAVASYEARASSEVASYQ-------SSVASLEVAIETARMDLQRQLLAYQSLLDVKLQLDAEISTYRNLLEGEAARCS 504
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Match: lmnb2 (lamin B2 [Source:ZFIN;Acc:ZDB-GENE-990630-13]) HSP 1 Score: 83.1889 bits (204), Expect = 8.994e-17 Identity = 102/408 (25.00%), Postives = 197/408 (48.28%), Query Frame = 2
Query: 449 VALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSK----------IKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKL-------RQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVY---------WQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTI-------TLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSD 1573
+ ++S ++ + R QEK L +LN+R A+YIE+ R LE +L+ + + + K ++ Y+ E+ ++R L D+ ++A LE+Q+ + E D+ R R S+Y+ + I E +L T D + EV LR+ + +L +Q E+E LA + LE+ + +L+E LEF K+IH+ E+ E + +E V ++ ++ AL +++ ++E ++ +QELD + ++L++ + + +++ + ++ ++ SR+ L Q + +H+L ++ E + +L + + +L + ++L L E + LLD KL L++EIAAY KLLE EE+R+N S +
Sbjct: 3 IEMKSLLSPTRVTRLQEKESLVELNDRLAAYIERVRSLEVDKARLLARVSMWEEEEEEQRQKSERQREATGGLRAAYDAELAEVRRALDDALGAKAALEIQLRLAQVERDEGRSRC-------SKYEQEWNVALGRIRDMEVQLCAKEASLSTASGDKEYLETEVDDLRNQVNQLEGVLVSSKQQLEEETLARVDLENRMLTLQEELEFTKRIHEQEIHESRS------KRETHVLEIDGGQQQAFEYKLSEALTELRQQHEEQVQLYRQELDQTMRAKLEKSILTPEKQSLDIS--------IREDLREATSRVGTLSSQLSAAQHDLALLQGRVRELEETLSHERDQSKRRLDESDRELAELREVMDEQLIEYEQLLDIKLALDMEIAAYRKLLEGEESRLNLSPE 389 HSP 2 Score: 65.0846 bits (157), Expect = 3.897e-11 Identity = 35/107 (32.71%), Postives = 57/107 (53.27%), Query Frame = 2
Query: 1922 TTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI---EEDGWGLGDTIVTSLYNAEGEEKAV 2233
+ G+I I E D+ G + + NN K + L G +KR I+ ++ +++ +R L +G+ +TIWA ++ + WG GD I TSL N++GEE AV
Sbjct: 438 SASGSIGIKEVDMEGKSVTMVNNSEKDQPLGGWVLKRQIE-DQAEIAYKFSSRYVLKAGQTVTIWAANAGVSHNPPSDLLWKTQSSWGTGDDIRTSLLNSDGEEVAV 543
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Match: desmb (desmin b [Source:ZFIN;Acc:ZDB-GENE-061027-102]) HSP 1 Score: 81.2629 bits (199), Expect = 1.310e-16 Identity = 93/358 (25.98%), Postives = 167/358 (46.65%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTK-ENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRV 1558
R EK +LQ+LN+RFA+YI+K R+LE QNK L+ E+ LKG ++ +++ +YE E+ +LR + +A +V+ +L E+ L++RL + + + R+D+ ++ R DL++ +++SL+E + F K+IHD E++E+ E V + ++ ALRDI+ EYE E + Y S+ ++ + R+N K+E + Q+Q+ + I L+ N E + + + + + + L++ + + ++ L + Q LL K+ L++EIA Y KLLE EE RV
Sbjct: 85 RTNEKVELQELNDRFANYIDKVRYLEQQNKALLAELALLKG---REPTRVGPLYEQELRELRAQVERLTGEKARGDVERANLLDELQRLQQRLQEETAAREEAEKNLQAFRQDV-------------------------DDATLARVDLER-----------------KVESLQEEIAFLKKIHDEELREVQVQVQEQTVHVEYDVGTKPDLTAALRDIRAEYEGIAAKNVAEAEEWYKSKFLDLSEAAVRNNEALRAAKQELMDYRRQVQAQQCDIDSLKGANESLERQIREMEERFSVESANYQDTITRLEDDIGNLKDEMARHLRDYQELLHVKMALDIEIATYRKLLEGEETRV 397
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010102.1 (pep scaffold:Pmarinus_7.0:GL478364:74479:82531:1 gene:ENSPMAG00000009106.1 transcript:ENSPMAT00000010102.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.4185 bits (202), Expect = 1.386e-16 Identity = 98/362 (27.07%), Postives = 169/362 (46.69%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRL----MMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVK--TLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENR 1555
R QEK ++ LN+RFA++I+K RFLE QNK L + L+ T +S I E++E IN LR Q+D L ++ L L ++ED ++Y++ I+ + + EGE V ++ K GA ++K + E + L D++ LR + +E E E QI T V L + N+ ++ + D++ +YE + E D+ Y + Q + + R++ E + E + + Q+Q R+ + L+ Q A+ E + E R + + L+E++ +Q++ + E Q L++ KL L++EI Y KLLE EE+R
Sbjct: 137 RTQEKEAIKHLNDRFANFIDKVRFLEQQNKVLEAQWYALQEKTTTGSS-IDEMFEAYINGLRR--------------QLDGLGHDKGQLNGNLGQMQAVVEDFRAKYEAEINNR----NAAEGEFVVVK--KDVDGAYLSKVELETRLQGLVDEINFLRSIFT----------------QELSELEAQIKHTNV--LVEI----DNSRNL-----DVDGIIADVRQQYETIAARSRAEADAFYQDKFQSLHSDSGRNDEELRMTRNEITELNRQMQRFRAEMEALKKQRAKLESAIAEAEGRGEADIREAKAAIAQLEEEIHKAKQEMARHVREYQELMNVKLALDIEIVTYRKLLEGEESR 450
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010061.1 (pep scaffold:Pmarinus_7.0:GL478364:48285:55057:1 gene:ENSPMAG00000009104.1 transcript:ENSPMAT00000010061.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 76.6406 bits (187), Expect = 5.317e-15 Identity = 99/369 (26.83%), Postives = 171/369 (46.34%), Query Frame = 2
Query: 473 SMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYE----IDDLRKRLMMLEDINSQYKSTID--KQRKDISGFEGELVELRRFKGTQGADVNKEKEEVK--TLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENR 1555
S+ R QEK ++ LN+RFA++I+K RFLE QNK L + L+ T S + E++E IN LR Q+D L +E DL + ++ED ++Y++ I +R+D E V + K GA ++K + E K L++D+ +R + +E E E QI T V L + N+ ++ + D++++YE + E D+ Y + Q + + R++ E + E + + Q+Q R+ + L+ Q A+ E + E R + + L+E++ +Q++ + E Q L++ KL L++EI Y KLLE EE+R
Sbjct: 130 SVLPTRTQEKEAIKHLNDRFANFIDKVRFLEQQNKVLEAQWYALQEKTTT-GSSVDEMFEAYINGLRR--------------QLDGLGHENGRANGDLGQMQAVVEDFRAKYEAEISLRTERED------EFVFAK--KDVDGAYLSKVELEAKLDGLQEDINFMRSIFA----------------QELSELEAQIKHTNV--LVEI----DNSRNL-----DVDGIIADVRHQYETIAARSRAEADAFYQDKFQSLHSDSGRNDEELRVTRNEITELNRQMQRFRAEMEALKKQRAKLESAIAEAEGRGEADIREAKAAIAQLEEEIHKAKQEMARHVREYQELMNVKLALDIEIVTYRKLLEGEESR 448
BLAST of Intermediate filament protein vs. Ensembl Nematostella
Match: EDO37225 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGD1]) HSP 1 Score: 91.6633 bits (226), Expect = 3.174e-19 Identity = 107/403 (26.55%), Postives = 195/403 (48.39%), Query Frame = 2
Query: 461 SGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQV-----DSLEY----------------EIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMV------YWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQN---AEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGN 1579
S S K RAQEK +LQ LN+R A+YI++ + LE +N +L +E+ + ++ +K +YE E+ R LL ++ K +A +++ D+ E+ E++D+RK +L D +Q + ++ ++ E L EL QG E + L+D LK + E+E L + LE+ QSL+E F+KQ++D E+ ++ + TK +V ++ M L++++ +Y++ K+E + Y S+ +E++ + RD+ +EEN + + + S++ LE +N +L +RA +E + L N +L + D L + + L+ K+ L++EI AY KLLE EE R+ + +
Sbjct: 28 SPPGSAKFTRAQEKAELQHLNDRLATYIDRVKNLEQENSKLRSEVTVSRKTVEREVDSMKSLYETELADARRLLDETAKEKAKQQIESSKNSNDAQEFKNKFDKEAAARKKAEKELNDVRK---LLHDKENQ----LTRRNQEALNLESVLREL------QG--------ECEELKDALKAAKYALEQETLTRVDLENRCQSLQEEQNFKKQMYDKELSDIRSQLKTVETKRVVVETDYKDKYEGLMAEKLQELREDYDSDARSFKEETELLYSSKFEELRIQRERDSEALAKLREENRNLSKSVDELSSQVHQLEAKNNALVSRVSDLQGLRAQDKEKHDNEILLRENEIAELRTSIDD---ALRDYEDLMGVKVALDMEITAYRKLLESEETRLEITPPAS 406 HSP 2 Score: 57.7658 bits (138), Expect = 1.168e-8 Identity = 35/108 (32.41%), Postives = 53/108 (49.07%), Query Frame = 2
Query: 1931 GNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEI---EEDGWGLGDTIVTSLYNAEGEEKAVHYQE 2245
G I EAD +G +I + N+G K E+L G TI+R + G ED + + L S +T+W+ ++ + WG G+ T+L NA GEE A +E
Sbjct: 442 GAIQFTEADPDGKYIKIYNSGEKDEALGGWTIQRQV-GTEDPSVYKFTPKYVLKSQSHVTVWSAQGGGTHKPPSDLVFKQLPSWGSGNEARTALVNAGGEEMATLLEE 548
BLAST of Intermediate filament protein vs. Ensembl Medaka
Match: viml (vimentin [Source:NCBI gene;Acc:101163931]) HSP 1 Score: 115.931 bits (289), Expect = 5.671e-27 Identity = 116/385 (30.13%), Postives = 194/385 (50.39%), Query Frame = 2
Query: 425 IPLIRGDSV--ALQSGVTS-MKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKEL--ATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVN 1561
+P + +S+ +L V S R EK +Q LN+RFASYIEK RFLE QNK L+ E+E+L+G GT S+I ++YE E+ +LR + +A +EV D+L +I+ LR++L R +IS ++EE + ++ RQD + ALA L LE +++SL+E + F K++HD E+ E + + +M + ++ ALRD++ +YEN T QE + Y S+ ++ + R+N K+E + + Q+Q++ + L+ N E L + N+ + + L+E + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R++
Sbjct: 63 MPRLASESLDFSLHDAVNSEFLTNRTNEKAQMQSLNDRFASYIEKVRFLEQQNKILLAELEQLRGKGT----SRIGDLYEDEMRELRRQVDQLTNEKARVEVHRDNLADDIERLREKL-----------------RDEIS----------------------QREEAEAT---MQSFRQDVDSAALARLDLERKIESLQEEINFLKKLHDEEMLEFQSQVQQQQQHVQVDMDVAKPDLTAALRDVRLQYENLATKNIQESEEWYKSKFADLTEAAARNNEALRMAKQEANDYRRQVQALTCEVDALKGTNESLERQLREMEENFSLESNGYQDTIGRLEEDMHNIKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRIS 401
BLAST of Intermediate filament protein vs. Ensembl Medaka
Match: viml (vimentin [Source:NCBI gene;Acc:101163931]) HSP 1 Score: 114.39 bits (285), Expect = 1.452e-26 Identity = 116/383 (30.29%), Postives = 192/383 (50.13%), Query Frame = 2
Query: 425 IPLIRGDSV--ALQSGVTS-MKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKG-GTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKEL--ATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENR 1555
+P + +S+ +L V S R EK +Q LN+RFASYIEK RFLE QNK L+ E+E+L+G GT S+I ++YE E+ +LR + +A +EV D+L +I+ LR++L R +IS ++EE + ++ RQD + ALA L LE +++SL+E + F K++HD E+ E + + +M + ++ ALRD++ +YEN T QE + Y S+ ++ + R+N K+E + + Q+Q++ + L+ N E L + N+ + + L+E + + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R
Sbjct: 63 MPRLASESLDFSLHDAVNSEFLTNRTNEKAQMQSLNDRFASYIEKVRFLEQQNKILLAELEQLRGKGT----SRIGDLYEDEMRELRRQVDQLTNEKARVEVHRDNLADDIERLREKL-----------------RDEIS----------------------QREEAEAT---MQSFRQDVDSAALARLDLERKIESLQEEINFLKKLHDEEMLEFQSQVQQQQQHVQVDMDVAKPDLTAALRDVRLQYENLATKNIQESEEWYKSKFADLTEAAARNNEALRMAKQEANDYRRQVQALTCEVDALKGTNESLERQLREMEENFSLESNGYQDTIGRLEEDMHNIKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESR 399
BLAST of Intermediate filament protein vs. Ensembl Medaka
Match: gfap (glial fibrillary acidic protein [Source:NCBI gene;Acc:101173548]) HSP 1 Score: 107.842 bits (268), Expect = 2.103e-24 Identity = 101/363 (27.82%), Postives = 179/363 (49.31%), Query Frame = 2
Query: 479 KKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
K+ R EK ++ LN+RFASYIEK R LE QNK LV E+ +L+G ++ S++ +IY+ E+ +LR + + +A LE++ D+L ++ L++RL Q V +++ E ++L RQD ++ +L+ + LE +++SL++ + F K+IH+ E++E ++ + ++ ALRDI+ +YEN T QE + Y S+ ++ + R+ K+E + + QIQ + + L N E L + + + L+E++ ++++ L E Q LL+ KL L++EIA Y KLLE EE+R+
Sbjct: 80 KETRTNEKMEMMGLNDRFASYIEKVRMLEQQNKVLVAELNQLRG---KEPSRLGDIYQDELRELRRQVDNLTTGKARLEIERDNLAADLATLKQRL-------------------------------------QDEIVQRQEAE-----NNLNAFRQDVDEASLSRVQLERKIESLQDEINFLKKIHEEELRESQEKVLAQQVHVDLDVSKPDLTAALRDIRLQYENLATSNMQETEEWYRSKFADLTDAANRNGEALRQAKQEANEYRRQIQVLTCDLDALRGTNESLERQLREMEDRCAMETAGYQDTVSRLEEEIQALKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEESRITIP 397
BLAST of Intermediate filament protein vs. Ensembl Medaka
Match: gfap (glial fibrillary acidic protein [Source:NCBI gene;Acc:101173548]) HSP 1 Score: 107.457 bits (267), Expect = 2.965e-24 Identity = 101/363 (27.82%), Postives = 179/363 (49.31%), Query Frame = 2
Query: 479 KKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFS 1567
K+ R EK ++ LN+RFASYIEK R LE QNK LV E+ +L+G ++ S++ +IY+ E+ +LR + + +A LE++ D+L ++ L++RL Q V +++ E ++L RQD ++ +L+ + LE +++SL++ + F K+IH+ E++E ++ + ++ ALRDI+ +YEN T QE + Y S+ ++ + R+ K+E + + QIQ + + L N E L + + + L+E++ ++++ L E Q LL+ KL L++EIA Y KLLE EE+R+
Sbjct: 80 KETRTNEKMEMMGLNDRFASYIEKVRMLEQQNKVLVAELNQLRG---KEPSRLGDIYQDELRELRRQVDNLTTGKARLEIERDNLAADLATLKQRL-------------------------------------QDEIVQRQEAE-----NNLNAFRQDVDEASLSRVQLERKIESLQDEINFLKKIHEEELRESQEKVLAQQVHVDLDVSKPDLTAALRDIRLQYENLATSNMQETEEWYRSKFADLTDAANRNGEALRQAKQEANEYRRQIQVLTCDLDALRGTNESLERQLREMEDRCAMETAGYQDTVSRLEEEIQALKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEESRITIP 397
BLAST of Intermediate filament protein vs. Ensembl Medaka
Match: desmb (desmin [Source:NCBI gene;Acc:101174730]) HSP 1 Score: 106.686 bits (265), Expect = 6.956e-24 Identity = 103/360 (28.61%), Postives = 178/360 (49.44%), Query Frame = 2
Query: 488 RAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTL-RDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNF 1564
R EK +LQ LN+RFASYIEK RFLE QN+ L E+E+L+G ++ ++I ++YE E+ +LR + R+ +EV+ D+L ++D L+ RL E+I Q++D E L R ADV+ TL R DL++ +++L+E + F K+IH+ E++EL T + M + ++ ALRDI+ +YE + + Y S++ ++ + +++N ++E + QIQ+ I L+ N + + + + + L+ ++ + ++ L E Q LL+ K+ L++EIA Y KLLE EE+R+
Sbjct: 104 RTNEKAELQHLNDRFASYIEKVRFLEQQNQALSVEVERLRG---REPTRIADLYEEEMRELRRQVEILTNQRSRVEVERDNLAEDLDKLKLRLQ--EEI---------LQKEDA---ENNLAAFR-------ADVD-----AATLARLDLER-----------------RIETLQEEIAFLKKIHEEEIRELQTQMQETQVQIQMDMSKPDLTAALRDIRTQYEGIAAKNIADAEDWYKSKVSDLNQAVSKNNEALKQARQETMEFRHQIQAYTCEIDSLKGTNESLMRQMRELEDRHGCEAARFQDTIARLEAEIANMKDEMARHLREYQDLLNIKMALDVEIATYRKLLEGEESRITM 417
BLAST of Intermediate filament protein vs. Planmine SMEST
Match: SMESG000030598.1 (SMESG000030598.1) HSP 1 Score: 741.11 bits (1912), Expect = 0.000e+0 Identity = 496/521 (95.20%), Postives = 510/521 (97.89%), Query Frame = 2
Query: 35 MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXITTSRSYYKPISQPRNNLKDLQKTSGMXXXXXXXXXXXXXNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNT--SQF 1591
MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQ FGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQL NEIEKLKGGTVQDNSKIKEIYEVEINQLR L+++SEKS+APLE QVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQ EYENKLTGIKQEL++SYG Q+QEI+TSKTRDNVETTHYKEENSR+KSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLL LQEQLTGTQQ+LT+ LTELQSLLDAKLGLELEIAAY+KLLEFEE+RVNFS+DGN SQF
Sbjct: 1 MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQVFGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLANEIEKLKGGTVQDNSKIKEIYEVEINQLRALVNESEKSKAPLEAQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQTEYENKLTGIKQELEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLLYLQEQLTGTQQELTVALTELQSLLDAKLGLELEIAAYAKLLEFEESRVNFSADGNATLSQF 521 HSP 2 Score: 146.747 bits (369), Expect = 2.541e-36 Identity = 75/127 (59.06%), Postives = 102/127 (80.31%), Query Frame = 2
Query: 1868 LSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEI 2248
++ E +YQ KRMKFRRTTKGNISIAE D+ G I+LEN GTK ESL GMTIKR +DG+E+ +SFTI + +L++GEK+++ AKGK TGKG+E+EI++ WG+G+ +VT+L ++EG EKA HYQE+
Sbjct: 553 MTERETTDYQSKRMKFRRTTKGNISIAETDLIGSTIILENIGTKVESLEGMTIKRVVDGSENKISFTISGKVNLNAGEKISLCAKGKITGKGREIEIDDASWGVGNNVVTTLCDSEGAEKAAHYQEV 679
BLAST of Intermediate filament protein vs. Planmine SMEST
Match: SMESG000030598.1 (SMESG000030598.1) HSP 1 Score: 740.725 bits (1911), Expect = 0.000e+0 Identity = 496/521 (95.20%), Postives = 510/521 (97.89%), Query Frame = 2
Query: 35 MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXITTSRSYYKPISQPRNNLKDLQKTSGMXXXXXXXXXXXXXNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNT--SQF 1591
MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQ FGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQL NEIEKLKGGTVQDNSKIKEIYEVEINQLR L+++SEKS+APLE QVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQ EYENKLTGIKQEL++SYG Q+QEI+TSKTRDNVETTHYKEENSR+KSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLL LQEQLTGTQQ+LT+ LTELQSLLDAKLGLELEIAAY+KLLEFEE+RVNFS+DGN SQF
Sbjct: 1 MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQVFGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLANEIEKLKGGTVQDNSKIKEIYEVEINQLRALVNESEKSKAPLEAQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQTEYENKLTGIKQELEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLLYLQEQLTGTQQELTVALTELQSLLDAKLGLELEIAAYAKLLEFEESRVNFSADGNATLSQF 521 HSP 2 Score: 146.747 bits (369), Expect = 2.653e-36 Identity = 75/127 (59.06%), Postives = 102/127 (80.31%), Query Frame = 2
Query: 1868 LSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEI 2248
++ E +YQ KRMKFRRTTKGNISIAE D+ G I+LEN GTK ESL GMTIKR +DG+E+ +SFTI + +L++GEK+++ AKGK TGKG+E+EI++ WG+G+ +VT+L ++EG EKA HYQE+
Sbjct: 554 MTERETTDYQSKRMKFRRTTKGNISIAETDLIGSTIILENIGTKVESLEGMTIKRVVDGSENKISFTISGKVNLNAGEKISLCAKGKITGKGREIEIDDASWGVGNNVVTTLCDSEGAEKAAHYQEV 680
BLAST of Intermediate filament protein vs. Planmine SMEST
Match: SMESG000030598.1 (SMESG000030598.1) HSP 1 Score: 730.324 bits (1884), Expect = 0.000e+0 Identity = 491/516 (95.16%), Postives = 505/516 (97.87%), Query Frame = 2
Query: 50 METSKKIAVKTTKQEVSGSGSSGGLNEQQAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXITTSRSYYKPISQPRNNLKDLQKTSGMXXXXXXXXXXXXXNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNT--SQF 1591
METSKKIAVKTTKQEVSGSGSSGGLNEQQ FGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQL NEIEKLKGGTVQDNSKIKEIYEVEINQLR L+++SEKS+APLE QVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQ EYENKLTGIKQEL++SYG Q+QEI+TSKTRDNVETTHYKEENSR+KSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLL LQEQLTGTQQ+LT+ LTELQSLLDAKLGLELEIAAY+KLLEFEE+RVNFS+DGN SQF
Sbjct: 1 METSKKIAVKTTKQEVSGSGSSGGLNEQQVFGSGSFRTGSGGVQISASGAIGSSSSGGVSGTGGVGSIGGGSGAGGEGTGASTVITTSRSYYKPISQPRNNLKDLQKTSGMSYSAQSNSQQGSSNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLANEIEKLKGGTVQDNSKIKEIYEVEINQLRALVNESEKSKAPLEAQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYEKEALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQTEYENKLTGIKQELEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEETQRSLELQLLYLQEQLTGTQQELTVALTELQSLLDAKLGLELEIAAYAKLLEFEESRVNFSADGNATLSQF 516 HSP 2 Score: 146.747 bits (369), Expect = 2.582e-36 Identity = 75/127 (59.06%), Postives = 102/127 (80.31%), Query Frame = 2
Query: 1868 LSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEI 2248
++ E +YQ KRMKFRRTTKGNISIAE D+ G I+LEN GTK ESL GMTIKR +DG+E+ +SFTI + +L++GEK+++ AKGK TGKG+E+EI++ WG+G+ +VT+L ++EG EKA HYQE+
Sbjct: 550 MTERETTDYQSKRMKFRRTTKGNISIAETDLIGSTIILENIGTKVESLEGMTIKRVVDGSENKISFTISGKVNLNAGEKISLCAKGKITGKGREIEIDDASWGVGNNVVTTLCDSEGAEKAAHYQEV 676
BLAST of Intermediate filament protein vs. Planmine SMEST
Match: SMESG000030596.1 (SMESG000030596.1) HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0 Identity = 358/432 (82.87%), Postives = 390/432 (90.28%), Query Frame = 2
Query: 302 ITTSRSYYKPISQPRNNLKDLQKTSGMXXXXXXXXXXXXXNIPLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNT--SQF 1591
+TT+ SY KPISQPRN+L+DLQKT+ + S N+ LIRGDSVALQSGVTSMKKGRAQEKR+LQ+LNERFASYIEKARFLEAQNKQL NEIEKLKGGTVQDNSKIKEIYEVEINQLR L+++SEKS+APLE QVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQR DI+ FEGELVEL+RFKGTQGADV EKEEVK+L+D LKKLRQDYE+EALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMV+WQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQ+QEIQTSKTRDNVETTHYKEENSRVKSQIQSMR RIA+LE QNAEYE NL NIR NYEETQR+L+ QL ++Q+QL+GTQQ+LT TELQSLLDAKLGLELEIAAY++LLE EE RV S+D + SQF
Sbjct: 49 VTTNHSYIKPISQPRNSLRDLQKTT-----------ENSQNM-LIRGDSVALQSGVTSMKKGRAQEKRNLQELNERFASYIEKARFLEAQNKQLANEIEKLKGGTVQDNSKIKEIYEVEINQLRALVNESEKSKAPLEAQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRNDIAAFEGELVELKRFKGTQGADVTLEKEEVKSLKDSLKKLRQDYEREALAHLTLEHELQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVFWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQMQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRMRIAELERQNAEYEINLKNIRDNYEETQRNLQSQLFSMQDQLSGTQQELTAASTELQSLLDAKLGLELEIAAYARLLEIEEERVLSSADQRSVMSQF 468 HSP 2 Score: 134.42 bits (337), Expect = 2.154e-32 Identity = 72/116 (62.07%), Postives = 92/116 (79.31%), Query Frame = 2
Query: 1901 KRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEI 2248
K+M RRTT GNISIAE+D +GLFIVLEN G++ +SL+GM IKRNIDG + V+++I + LS+GE++TI GKKTGKGKELEIEED WG G+ ++T+L +A GEEKA YQEI
Sbjct: 535 KKMSSRRTTTGNISIAESDESGLFIVLENMGSQTQSLSGMMIKRNIDGVANFVTYSIPKKIDLSAGERITICGHGKKTGKGKELEIEEDSWGFGENVITALCDANGEEKAALYQEI 650
BLAST of Intermediate filament protein vs. Planmine SMEST
Match: SMESG000030599.1 (SMESG000030599.1) HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0 Identity = 342/431 (79.35%), Postives = 392/431 (90.95%), Query Frame = 2
Query: 302 ITTSRSYYKPISQPRNNLKDLQKTSGMXXXXXXXXXXXXXNI-PLIRGDSVALQSGVTSMKKGRAQEKRDLQDLNERFASYIEKARFLEAQNKQLVNEIEKLKGGTVQDNSKIKEIYEVEINQLRTLLSDSEKSRAPLEVQVDSLEYEIDDLRKRLMMLEDINSQYKSTIDKQRKDISGFEGELVELRRFKGTQGADVNKEKEEVKTLRDDLKKLRQDYXXXXXXXXXXXXXXQSLKEALEFEKQIHDTEVKELATVAYRDPTKENMVYWQSEMGRALRDIQNEYENKLTGIKQELDSSYGSQLQEIQTSKTRDNVETTHYKEENSRVKSQIQSMRSRIADLEMQNAEYEHNLNNIRANYEEXXXXXXXXXXXXXXXXXXXXXDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTSQF 1591
++T+RS YKPISQ RNN KDLQ+ + + + Q S ++ PLIRGDSVALQ+G+ S+KKGRAQEK+DLQ+LNERFASYIEKARFLE QNKQLV EIE+LKGGT+QD SK+KEIYEVEINQLR+LL++SEKSRAPLE+QV+SLEYEI DL+KRL++LEDI +QY++TIDKQR+++ FEGE+VEL+RFK T+ D KEK+E LRD++KKLRQ+YEKEALAH+TLEH+LQSL+E +EFEKQIH TEVKELATVAY++PTKENMVYWQSEMGRALRDIQ EYENKL+GIKQEL++SY Q+QEI+TSKTRDNVETT YKEENSR+KSQIQSMRSRI DLEMQNAEYEHNLNNIRANYEETQRSLELQLLNLQEQLTGTQQDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTSQF
Sbjct: 103 VSTTRSMYKPISQSRNNFKDLQRNAASLSYSMQSGNQQSGSVMPLIRGDSVALQTGLNSIKKGRAQEKKDLQELNERFASYIEKARFLEEQNKQLVGEIEELKGGTIQDTSKVKEIYEVEINQLRSLLNESEKSRAPLEIQVNSLEYEIGDLQKRLLLLEDIGTQYRATIDKQRRELETFEGEMVELKRFKDTRSIDATKEKDETSNLRDEIKKLRQNYEKEALAHITLEHDLQSLRETMEFEKQIHATEVKELATVAYQNPTKENMVYWQSEMGRALRDIQTEYENKLSGIKQELEASYSLQMQEIRTSKTRDNVETTQYKEENSRIKSQIQSMRSRITDLEMQNAEYEHNLNNIRANYEETQRSLELQLLNLQEQLTGTQQDLTITLTELQSLLDAKLGLELEIAAYSKLLEFEENRVNFSSDGNTSQF 533 HSP 2 Score: 261.536 bits (667), Expect = 7.226e-76 Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 2
Query: 1847 LRFNINELSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAXXXXXXXXXELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEIQG 2254
LRFNINELSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAKGKKTGKGKELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEIQG
Sbjct: 619 LRFNINELSSTEAEEYQLKRMKFRRTTKGNISIAEADINGLFIVLENNGTKFESLTGMTIKRNIDGNEDAVSFTIQNRTSLSSGEKLTIWAKGKKTGKGKELEIEEDGWGLGDTIVTSLYNAEGEEKAVHYQEIQG 754 The following BLAST results are available for this feature:
BLAST of Intermediate filament protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Intermediate filament protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Intermediate filament protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Intermediate filament protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Intermediate filament protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Intermediate filament protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026612 ID=SMED30026612|Name=Intermediate filament protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2359bpback to top protein sequence of SMED30026612-orf-1 >SMED30026612-orf-1 ID=SMED30026612-orf-1|Name=SMED30026612-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=741bp MLAIEMETSKKIAVKTTKQEVSGSGSSGGLNEQQAFGSGSFRTGSGGVQIback to top Aliases
The feature 'Intermediate filament protein' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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