Otopetrin-2-like isoform X1 isoform 2

Overview
NameOtopetrin-2-like isoform X1 isoform 2
Smed IDSMED30026581
Length (bp)1138
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Otopetrin-2-like isoform X1 isoform 2 (SMED30026581) t-SNE clustered cells

Violin plots show distribution of expression levels for Otopetrin-2-like isoform X1 isoform 2 (SMED30026581) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30026581

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30026581h1SMcG0009874 SmedASXL_008692SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30026581h1SMcG0009874 SmedASXL_000905SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30026581h1SMcG0009874 dd_Smed_v4_7349_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026581h1SMcG0009874 dd_Smed_v4_7349_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30026581h1SMcG0009874 dd_Smed_v4_16909_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30026581h1SMcG0009874 dd_Smed_v4_25133_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30026581h1SMcG0009874 dd_Smed_v6_16909_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30026581h1SMcG0009874 dd_Smed_v6_25133_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30026581h1SMcG0009874 DN308442smed_ncbi_20240424PMID:25356635
Cowles et al., 2014		whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30026581h1SMcG0009874 DN308442smed_ncbi_20240424PMID:25356635
Cowles et al., 2014		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Match: A0A4E0RKK5 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_002020 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 4.154e-12
Identity = 46/149 (30.87%), Postives = 80/149 (53.69%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRR-GMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDF---QKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVK 929
            +F I ++A+   R   +  R+SN+   + IY   + SF I+QT F L  HRL++L + ++  +  +H +  N+ +W+  +I K+S        SE  + +   ++     YFLPA+ EY  I +A+ YE   RIG+ K +   H+K+ K
Sbjct:  136 IFAIMAIAFASCRAYEVSMRKSNY-LLVSIYTASKISFFIVQTVFFLVLHRLVVLANVRMFSVILLHTLTVNLCVWVESAIEKVSLAMIVEHSSENIADDSRGYTLAATHYFLPAIPEYCAIGVAVTYEMAQRIGQLKQIEAHHEKTEK 283

HSP 2 Score: 38.891 bits (89), Expect = 4.154e-12
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 1 
Query:  355 YIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGI 474
            Y+  NR  T EA E+G  +  +++ S +GE V+LYLR  I
Sbjct:   95 YVVHNRHATIEAIERGDLSATRILFSMRGEPVSLYLRFAI 134
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Match: A0A504X7Y3 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_04231 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 7.010e-12
Identity = 45/149 (30.20%), Postives = 80/149 (53.69%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRR-GMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDF---QKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVK 929
            +F I ++A+   R   +  R+SN+   + IY   + SF I+QT F L  HRL++L + ++  +  +H +  N+ +W+  +I K+S        +E  + +   ++     YFLPA+ EY  I +A+ YE   RIG+ K +   H+K+ K
Sbjct:  136 IFAIMAIAFASCRAYEVSMRKSNY-LLVSIYTASKISFFIVQTVFFLVLHRLVVLANVRMFSVILLHTLTVNLCVWVESAIEKVSLAMIVEHSAENIADDARGYTLTATHYFLPAIPEYCAIGVAVTYEMAQRIGQLKQIEAHHEKTEK 283

HSP 2 Score: 38.891 bits (89), Expect = 7.010e-12
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 1 
Query:  355 YIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGI 474
            Y+  NR  T EA E+G  +  +++ S +GE V+LYLR  I
Sbjct:   95 YVVHNRHATIEAIERGDLSATRILFSMRGEPVSLYLRFAI 134
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Match: A0A074ZN56 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_04439 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.332e-11
Identity = 50/172 (29.07%), Postives = 78/172 (45.35%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDFQ-------KSEFCSKNKYDFSCNLY-------------------IYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKP 932
            +FG+ S+A++ ++     RE   D  + +  +++ +F  +QT FIL  HRL+IL H ++M    VHL+ TN+ +W   S+GKI               F   N      NLY                    Y LP +SEY ++  A++YE   RIG+   +     K  KP
Sbjct:  144 MFGVMSIAHVAVKFAEDTREKRNDVLLYLKSILEMAFFALQTLFILRYHRLVILRHNEVMGAGLVHLLTTNLCMWADISVGKIDKTLSVGGHKIGPLAFVESNGAANLVNLYNQSQYMKSRSDEEGFFGVSFYLLPTVSEYCLLAAALLYEITVRIGQPSFIEIEKVKDGKP 315

HSP 2 Score: 36.5798 bits (83), Expect = 1.332e-11
Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = 1 
Query:  367 NRFQTSEAAEKGRH-ANYKLVLSQKGEKVNLYLRLGI 474
            +R QT EA  +G+    + +  S KGEKVNLYLRLG+
Sbjct:  106 HRGQTFEAVHRGKGPVPFSVKFSVKGEKVNLYLRLGL 142
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Match: A0A183A6V6 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2691 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.590e-11
Identity = 40/141 (28.37%), Postives = 75/141 (53.19%), Query Frame = 3 
Query:  573 IIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDF---QKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVI 986
            I IY   +  F +IQ  F L  HRL++L + +I  +  +H++  N+ +W+  +I K+S      + ++  S +K  ++     YFLPA+ EY  + +A+ YE   RIG+ K +   H+K+ K +        V ++ G+++
Sbjct:  128 ISIYTTSKILFFVIQPIFFLVLHRLVVLANVRIFSVILLHILTVNLCVWVESAIEKVSMTINTERSTDGHSSDKRGYTLAATHYFLPAIPEYCAVAVAVTYEMAQRIGQLKQIEAHHEKAEKQEKRPQLQTYVGLMLGSIL 268

HSP 2 Score: 40.4318 bits (93), Expect = 1.590e-11
Identity = 18/40 (45.00%), Postives = 26/40 (65.00%), Query Frame = 1 
Query:  355 YIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGI 474
            Y+  NR  T EA EKG  +  +++ S +GE V+LYLR+ I
Sbjct:   61 YVAHNRHATIEAIEKGDLSATRILFSMRGEPVSLYLRIAI 100
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Match: G7Y5Q2 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_101421 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 7.140e-11
Identity = 46/178 (25.84%), Postives = 83/178 (46.63%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITN-------------------------XXXXXXXXXXXXXXDFQKSEFCSKNKYDFSC--NLYIYFLPALSEYAVIVIAIMYEFYTRIGKY---KLLRFGHKKSVKPKF 938
            +FG+ S+A++ ++     RE   D  + +  +++ +F  +QT FIL  HRL+IL H ++M    VHL+ TN                         +    S       + + +S++ +K++ D      +  Y LP +SEY ++  A++YE   RIG+    ++ +    KS K KF
Sbjct:  107 MFGVMSIAHVAVKFAEDTREKRNDVLLYLKSILEMAFFALQTLFILRYHRLVILRHNEVMGAGLVHLLTTNLCMWADISVGKIDKTLSVGGHKIGSLAFVESNGAANFVNPYNQSQY-TKSQSDEEGFFGVSFYLLPTVSEYCLLAAALLYEITVRIGQPSFIEIEKVKDGKSHKNKF 283

HSP 2 Score: 36.5798 bits (83), Expect = 7.140e-11
Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = 1 
Query:  367 NRFQTSEAAEKGR-HANYKLVLSQKGEKVNLYLRLGI 474
            +R QT EA  +G+    + +  S KGEKVNLYLRLG+
Sbjct:   69 HRGQTFEAVHRGKGPVPFSVKFSVKGEKVNLYLRLGL 105
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1)

HSP 1 Score: 343.969 bits (881), Expect = 6.796e-129
Identity = 213/214 (99.53%), Postives = 214/214 (100.00%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRXXXXXXXXXXXXXXXXXXXXXXXFTYLNA 1136
            +FGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRIWIFHFHNIEIIILNLLSIIILIFTYLNA
Sbjct:  162 IFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRIWIFHFHNIEIIILNLLSIIILIFTYLNA 375

HSP 2 Score: 136.732 bits (343), Expect = 6.796e-129
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 
Query:  175 RKNNEKIGIFXXXXXXXXXXXXXXXXGASFQNASENLLFVNVYYXXXXXXXXXXXXXXWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 477
            RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII
Sbjct:   61 RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 161
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1)

HSP 1 Score: 343.199 bits (879), Expect = 9.560e-129
Identity = 213/214 (99.53%), Postives = 214/214 (100.00%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRXXXXXXXXXXXXXXXXXXXXXXXFTYLNA 1136
            +FGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRIWIFHFHNIEIIILNLLSIIILIFTYLNA
Sbjct:  117 IFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAGAVIVLFCIFTSISSVVFHVLEIDRIWIFHFHNIEIIILNLLSIIILIFTYLNA 330

HSP 2 Score: 136.732 bits (343), Expect = 9.560e-129
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 
Query:  175 RKNNEKIGIFXXXXXXXXXXXXXXXXGASFQNASENLLFVNVYYXXXXXXXXXXXXXXWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 477
            RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII
Sbjct:   16 RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 116
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1)

HSP 1 Score: 280.026 bits (715), Expect = 1.373e-109
Identity = 156/173 (90.17%), Postives = 160/173 (92.49%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNXXXXXXXXXXXXXXDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNS---CMIVSMIAGAVIVLFCIF 1004
            +FGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNS    + VS I      + C+F
Sbjct:  117 IFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMKLCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPALSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSYKNILEVSFIFSLNFSILCLF 289

HSP 2 Score: 136.346 bits (342), Expect = 1.373e-109
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 
Query:  175 RKNNEKIGIFXXXXXXXXXXXXXXXXGASFQNASENLLFVNVYYXXXXXXXXXXXXXXWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 477
            RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII
Sbjct:   16 RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 116
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1)

HSP 1 Score: 136.346 bits (342), Expect = 7.701e-58
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 
Query:  175 RKNNEKIGIFXXXXXXXXXXXXXXXXGASFQNASENLLFVNVYYXXXXXXXXXXXXXXWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 477
            RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII
Sbjct:   61 RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 161

HSP 2 Score: 107.071 bits (266), Expect = 7.701e-58
Identity = 49/51 (96.08%), Postives = 51/51 (100.00%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRL 647
            +FGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHR+
Sbjct:  162 IFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRI 212
[Close]
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1)

HSP 1 Score: 135.961 bits (341), Expect = 9.723e-58
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 
Query:  175 RKNNEKIGIFXXXXXXXXXXXXXXXXGASFQNASENLLFVNVYYXXXXXXXXXXXXXXWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 477
            RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII
Sbjct:   16 RKNNEKIGIFLYSSILLTIGISSTSYGASFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRHANYKLVLSQKGEKVNLYLRLGII 116

HSP 2 Score: 107.071 bits (266), Expect = 9.723e-58
Identity = 49/51 (96.08%), Postives = 51/51 (100.00%), Query Frame = 3 
Query:  495 VFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRL 647
            +FGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHR+
Sbjct:  117 IFGISSLAYIGMRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRI 167
The following BLAST results are available for this feature:
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RKK54.154e-1230.87Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A504X7Y37.010e-1230.20Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A074ZN561.332e-1129.07Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A183A6V61.590e-1128.37Uncharacterized protein OS=Echinostoma caproni OX=... [more]
G7Y5Q27.140e-1125.84Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Otopetrin-2-like isoform X1 isoform 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000051187.16.796e-12999.53SMESG000051187.1[more]
SMESG000051187.19.560e-12999.53SMESG000051187.1[more]
SMESG000051187.11.373e-10990.17SMESG000051187.1[more]
SMESG000051187.17.701e-58100.00SMESG000051187.1[more]
SMESG000051187.19.723e-58100.00SMESG000051187.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026581 ID=SMED30026581|Name=Otopetrin-2-like isoform X1 isoform 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1138bp
GTAGAAATAGGTCAAAAAGTATGTGAACAATTTCTGATTCACAGAAAGTA
TATAGAAATAGGAAAAAAATAATTTATTATACCTAGTATTCTAAAATTTT
GTTTTAAAATGTCGCTTTCGAACGTTGATGACTATCAAGACAATAAAGGA
AATGGAAATGATACAGATATTTTTAGAAAAAATAATGAGAAAATAGGCAT
TTTTCTCTACTCTTCAATTCTTTTAACCATCGGGATCTCATCAACATCAT
ACGGAGCTTCTTTTCAAAACGCCAGTGAAAATCTATTGTTTGTGAATGTA
TATTATAGCATAATGTTTATCAGTTCATCATTAATTATGATCGGAATGTG
GATATATATCTATAGAAATAGATTTCAAACTTCGGAAGCCGCCGAAAAAG
GGAGACATGCAAATTACAAACTTGTTCTTTCCCAGAAAGGCGAAAAAGTA
AACCTGTATCTGAGACTGGGAATAATAAGTACGTATAATTCTAAGTATTT
GGAATCTCAAGCCTGGCGTACATTGGAATGAGAAGGGGTATGAGATTCCG
TGAAAGCAATTTCGATGCGAGTATAATAATTTACGGTGTTATTCAAGGAT
CCTTCATTATTATTCAAACATGTTTCATTTTGTTTAATCACAGACTTATA
ATTTTAACCCATAAGAAAATCATGAAGCTCTGTTTTGTCCACTTGATTAT
AACAAACATTTTGATTTGGATCAGCGGTTCAATTGGGAAAATTTCATCGG
ATTTTCAAAAATCGGAATTTTGTTCAAAAAATAAATATGACTTTTCCTGC
AATCTTTACATTTACTTTCTTCCAGCTTTGAGTGAATATGCAGTTATTGT
GATCGCCATTATGTATGAATTTTATACACGAATCGGAAAGTACAAGTTGT
TGAGATTTGGACATAAAAAGTCTGTCAAACCAAAGTTCTCAGTAAATAGC
TGTATGATAGTATCAATGATAGCAGGAGCTGTTATTGTACTTTTTTGTAT
ATTTACTTCTATTTCTTCGGTCGTTTTTCATGTTCTTGAAATTGACAGAA
TTTGGATCTTTCATTTTCACAATATCGAAATAATAATTCTAAATTTATTA
AGCATAATAATTTTAATTTTTACCTATTTGAATGCAAG
back to top

protein sequence of SMED30026581-orf-1

>SMED30026581-orf-1 ID=SMED30026581-orf-1|Name=SMED30026581-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=203bp
MRRGMRFRESNFDASIIIYGVIQGSFIIIQTCFILFNHRLIILTHKKIMK
LCFVHLIITNILIWISGSIGKISSDFQKSEFCSKNKYDFSCNLYIYFLPA
LSEYAVIVIAIMYEFYTRIGKYKLLRFGHKKSVKPKFSVNSCMIVSMIAG
AVIVLFCIFTSISSVVFHVLEIDRIWIFHFHNIEIIILNLLSIIILIFTY
LNA
back to top

protein sequence of SMED30026581-orf-2

>SMED30026581-orf-2 ID=SMED30026581-orf-2|Name=SMED30026581-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=141bp
MSLSNVDDYQDNKGNGNDTDIFRKNNEKIGIFLYSSILLTIGISSTSYGA
SFQNASENLLFVNVYYSIMFISSSLIMIGMWIYIYRNRFQTSEAAEKGRH
ANYKLVLSQKGEKVNLYLRLGIISTYNSKYLESQAWRTLE*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000025nervous system
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR004878Otopetrin
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 50..69
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 179..201
NoneNo IPR availableTMHMMTMhelixcoord: 137..159
NoneNo IPR availableTMHMMTMhelixcoord: 94..116