FLYWCH-type zinc finger-containing protein 1

Overview
NameFLYWCH-type zinc finger-containing protein 1
Smed IDSMED30026565
Length (bp)1727
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of FLYWCH-type zinc finger-containing protein 1 (SMED30026565) t-SNE clustered cells

Violin plots show distribution of expression levels for FLYWCH-type zinc finger-containing protein 1 (SMED30026565) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FLYWCH-type zinc finger-containing protein 1 (SMED30026565) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FLYWCH-type zinc finger-containing protein 1 (SMED30026565) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026565

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30026565 dd_Smed_v4_11413_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026565 dd_Smed_v4_11413_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026565 dd_Smed_v4_11413_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30026565 dd_Smed_v4_11413_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Celegans
Match: F09E5.10 (pep chromosome:WBcel235:II:5348096:5350546:-1 gene:WBGene00017288.1 transcript:F09E5.10.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F09E5.10)

HSP 1 Score: 58.151 bits (139), Expect = 1.060e-8
Identity = 39/127 (30.71%), Postives = 63/127 (49.61%), Query Frame = 3 
Query:  621 DGTFRVSPNGYDQLY--TIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVP 995
            DG F   P  Y QLY  T+         P+V+AL  S  E+ Y+   + + +L     P     DFE+A++ A ++ +P A I GC  HF   +++ L+     +   N+P+F  L+K +  L+F P
Sbjct:  433 DGKFSEIPKNYRQLYIATVTDLDTNVAVPLVFALCGSAKEDLYRRK-RWLYLLNLSTYP-----DFEVASVNASRAVWPNADIFGCWRHFKVNLFKNLRKAVGDD--INKPEFVNLLKSIYTLSFAP 551
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Match: A0A0C2J8U0 (Uncharacterized protein OS=Thelohanellus kitauei OX=669202 GN=RF11_00573 PE=4 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 4.493e-83
Identity = 157/457 (34.35%), Postives = 246/457 (53.83%), Query Frame = 3 
Query:  108 LEFVTSQKGNRKLIVQSYLFTKHSENSDGKSLWRCEKRTGTARVHTKDDEIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAAHLLPKRTTLARDVRRHRQKVGPND------QDDIMRYSLTQSNXXXX-------XXXXXXXXXXAAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIED-PAYRDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQ-YSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITYLGDIA 1433
            ++ + SQKG  +++   + + +   N DG SLWRC K     R+  K D  V   + H H     + E EK +A ++ R +   E  R I+ N  + V + AA+ LP      R + R R++   N+      Q+ I+   L  + R              + M +F   ++L  L    HWF DGTF+++P  + Q++TIH  +N  V P++Y L+  +T+  Y+ + + ++ L+  L+P SIMVDFE   + A  + FP +T+ GC+FH GQ +WR++Q EG +  YR++ +  L  K L+AL+F+PP+DV   F+ L E  PA  ++  + +Y ED +IGR+RR RR  P F I +W+   RV D LPR+NN++EGWHNAF + VG  HPT  KL    + EQ  + Q + R      T    K  YI+++  L  ++  Y  RD I YL  ++
Sbjct:    1 MDIIKSQKGKEQILCGGFRYRRDKLNQDGSSLWRCVKSECLGRLKKKTDGTVQITSEHEHAPDTAKNESEKIKADIRERAKYGVEKPRQIIVNSSVGVTLEAANFLPSYIASQRTIERKRKRPDVNNSRPNTVQEIILPEELKLTTRSQNFLLWDSGNEDTNRMFMFGTSNNLQLLENNPHWFMDGTFKIAPEIFLQVFTIHALVNNRVIPLIYVLMGKKTQADYERVFERVVELRPSLSPMSIMVDFEKGCMNALLNAFPNSTVHGCLFHLGQSLWRRIQREGLTNSYRDDENVKLYSKMLIALSFIPPEDVGTAFDELSESRPA--NLHNLYNYWEDTYIGRMRRNRRDDPLFPISMWNMRGRVADGLPRTNNSVEGWHNAFQSSVGCHHPTIYKLIENFRLEQDLAEQTISRISSGINTQPASKSKYIQLSRRLAAILPTYEGRDLIEYLRAVS 455
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Match: A0A0C2IYG6 (Uncharacterized protein OS=Thelohanellus kitauei OX=669202 GN=RF11_14163 PE=4 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 7.331e-83
Identity = 157/457 (34.35%), Postives = 246/457 (53.83%), Query Frame = 3 
Query:  108 LEFVTSQKGNRKLIVQSYLFTKHSENSDGKSLWRCEKRTGTARVHTKDDEIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAAHLLPKRTTLARDVRRHRQKVGPND------QDDIMRYSLTQSNXXXX-------XXXXXXXXXXAAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIED-PAYRDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQ-YSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITYLGDIA 1433
            ++ + SQKG  +++   + + +   N DG SLWRC K     R+  K D  V   + H H     + E EK +A ++ R +   E  R I+ N  + V + AA+ LP      R + R R++   N+      Q+ I+   L  + R              + M +F   ++L  L    HWF DGTF+++P  + Q++TIH  +N  V P++Y L+  +T+  Y+ + + ++ L+  L+P SIMVDFE   + A  + FP +T+ GC+FH GQ +WR++Q EG +  YR++ +  L  K L+AL+F+PP+DV   F+ L E  PA  ++  + +Y ED +IGR+RR RR  P F I +W+   RV D LPR+NN++EGWHNAF + VG  HPT  KL    + EQ  + Q + R      T    K  YI+++  L  ++  Y  RD I YL  ++
Sbjct:    1 MDIIKSQKGKEQILCGGFRYRRDKLNQDGSSLWRCVKSECLGRLKKKTDGTVQITSEHEHAPDTAKNESEKIKADIRERAKYGVEKPRQIIVNSSVGVTLEAANFLPSYIASQRTIERKRKRPDVNNSRPNTVQEIILPEELKLTTRSQNFLLWDSGNEDTNRMFMFGTSNNLQLLENNPHWFMDGTFKIAPEIFLQVFTIHALVNNRVIPLIYVLMGKKTQADYERVFERVVELRPSLSPMSIMVDFEKGCMNALLNAFPNSTVHGCLFHLGQSLWRRIQREGLTNSYRDDENVKLYSKMLIALSFIPPEDVGTAFDELSESRPA--NLHNLYNYWEDTYIGRMRRNRRDDPLFPISMWNMRGRVADGLPRTNNSVEGWHNAFQSSVGCHHPTIYKLIENFRFEQDLAEQTISRISSGINTQPASKSKYIQLSRRLAAILPTYEGRDLIEYLRAVS 455
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Match: A0A5N4B0X7 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_00217 PE=4 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 5.821e-80
Identity = 158/449 (35.19%), Postives = 240/449 (53.45%), Query Frame = 3 
Query:  144 LIVQSYLFTKHSENSDGKSLWRC---EKRTGTARVHTKDDEIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNV--PVSAAHLLPKRTTLARDVRRHRQKVG-----PN-----DQDDIMRYSLTQSNXXXXXX------XXXXXXXXAAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAYR---DIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQYSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITY 1418
            L+++ YLF K     DG+  W+C   +K   +AR HT +D +V  +  HNH      +E  KA   +K R +TT +   +++  E   +  PV A   LP    + R +RR RQ  G     PN     +  D MR       +    +          ++IF+ Q +L  L RCEHW+ADGTF+ +P  + Q+YTIHG       P V+ LL  +++  YQ ++Q +  L+  L P++IM+DFE AAI AFQ+ FP   + GC FH GQC+WRK+Q  G + RY N+ +FA+ VK L A++F+P  DV+  FE L++   YR   D+  ICDY ED +IGR  R  R PP F   LW+ +    ++LPR+NN++EGWH  F  ++   HP+  +    +++EQ+ N++       G    ++KK Y  +N  +   V  Y+  +   Y
Sbjct:    2 LVLEGYLFEKDKMADDGRIFWKCVEAKKGKCSARRHTLNDAVVKSLLDHNHVPDIAAIEARKAINSIKERAKTTRDAGYAVIAVESSQLLEPVQAK--LPNVECIKRTIRRQRQLQGNYPANPNSLRELNIPDDMRLICVNGQQEPFLLFDNGPDTEHRILIFSTQRNLELLERCEHWYADGTFKTTPPLFTQVYTIHGVRFNTCIPSVFVLLPGKSKVLYQEMIQALKGLRPNLKPSTIMIDFERAAIEAFQTEFPEILVRGCFFHLGQCIWRKVQEHGKAVRYANDVEFAVQVKMLTAISFLPLHDVVLGFEELMDTEYYRNEIDLHPICDYFEDVWIGRPHRNIRRPPLFQYNLWNFYDATNNDLPRTNNSVEGWHRRFTELISAQHPSIWRFIAAIKEEQHMNEIAISHYIQGRDGPRQKKKYRDLNIRIKNCVARYDANEKFLY 448
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Match: A0A0C2IP16 (Uncharacterized protein OS=Thelohanellus kitauei OX=669202 GN=RF11_13865 PE=4 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 2.992e-78
Identity = 155/457 (33.92%), Postives = 241/457 (52.74%), Query Frame = 3 
Query:  108 LEFVTSQKGNRKLIVQSYLFTKHSENSDGKSLWRCEKRTGTARVHTKDDEIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAAHLLPKRTTLARDVRRHRQKVGPND------QDDIMRYSLTQSNXXXX-------XXXXXXXXXXAAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIED-PAYRDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQ-YSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITYLGDIA 1433
            ++ + SQKG  +++   + + +   N DG SLWRC K     R+  K D  V   + H H     + E EK +A ++ R +   E  R I+ N  + V + AA+ LP      R + R R++   N+      Q+ I+   L  + R              + M +F   ++   L    HWF DGTF+++P  + Q++TIH  +N  V P++YA         Y+ + + ++ L+  L+P SIMVDFE   + A  + FP +T+ GC+FH GQ +WR++Q EG +  YR++ +  L  K L+AL+F+PP+DV   F+ L E  PA  ++  + +Y ED +IGR+RR RR  P F I +W+   RV D LPR+NN++EGWHNAF + VG  HPT  KL    + EQ  + Q + R      T    K  YI+++  L  ++  Y  RD I YL  ++
Sbjct:    1 MDIIKSQKGKEQILCGGFRYRRDKLNQDGSSLWRCVKSECLGRLKKKTDGTVQITSEHEHAPDTAKNESEKIKADIRERAKYGVEKPRQIIVNSSVGVTLEAANFLPSYIASQRTIERKRKRPDVNNSRPNTVQEIILPEELKLTTRSQNFLLWDSGNEDTNRMFMFGTSNNCKLLENNPHWFMDGTFKIAPEIFLQVFTIHALVNNRVIPLIYA--------DYERVFERVVELRPSLSPMSIMVDFEKGCMNALLNAFPNSTVHGCLFHLGQSLWRRIQREGLTNSYRDDENVKLYSKMLIALSFIPPEDVGTAFDELSESRPA--NLHNLYNYWEDTYIGRMRRNRRDDPLFPISMWNMRGRVADGLPRTNNSVEGWHNAFQSSVGCHHPTIYKLIENFRLEQDLAEQTISRISSGINTQPASKSKYIQLSRRLAAILPTYEGRDLIEYLRAVS 447
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Match: A0A5N4APK1 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_07018 PE=4 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 1.533e-77
Identity = 168/470 (35.74%), Postives = 254/470 (54.04%), Query Frame = 3 
Query:   93 KVTTMLEFVTSQKGNRKLIVQS---YLFTKHSENSDGKSLWRCEKR-TGTARVHTKDD--EIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAAHLLPKRTTLARDVRRHRQKVG----PNDQDDI---MRYSLTQSNXXXXXXXXXXXXXX---------AAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAY-RDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQYSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITYLGDIA 1433
            +VT ++ FVTS +  RK I  +   Y+F+K S N +  S+W CEKR     R+ T  +  E+V  V  HNH     +  V    + +K R   T+ET + I+   L NV  +    LP++  + R +RR R + G    P D + +   ++Y + + + L  +  + D             A  D+L  L+R + W+ DGTF V+PN + QLYTIH    G V P +YALL ++T+E YQ LLQ++  L  GL P  +++DFE AA+ AF   F    ++GC FH  Q VWR++Q+EG    Y+   DFA  V+ + A+AFVPP  V++ F+ L+E   +  + + I DY ED +IGR  RR+R PP FSI++W+ + R +D   R+NN IEGWH  F +V     P   K    L+++Q  +     Q   G    +  K Y  ++  +H +V DY+NR+ I YL  I+
Sbjct:   25 EVTPIMSFVTSSRDGRKFIDDNNFVYVFSKKSANKEN-SIWVCEKRGLCKGRIWTIANRVEVVRIVTPHNHAAEATRGPVLNILSRVKERARDTEETPQQILATNLQNVHRNIMAKLPRQDAIKRTIRRQRNQQGLPELPTDLEHLNIPLQYRVIEVDGLEQQFLKYDSQDTLLPDRILIFATDDNLNMLNRSDSWYGDGTFSVAPNLFFQLYTIHVEQYGTVTPAIYALLPNKTKETYQKLLQQLKNLIPGLLPTRVLLDFEAAAMGAFSEEFEGIAVSGCFFHLCQNVWRRVQSEGLQMLYQGNHDFAQWVRMIPAIAFVPPHRVLEAFDELVEYENFPPEAQPIVDYFEDIYIGRRARRQRRPPIFSIEMWNMYQRTLDGRHRTNNNIEGWHRGFQSVCD-TSPNVFKFIESLKKQQTIHAYQINQFITGAPPPRPNKRYATISARIHVIVNDYDNRNIIDYLRGIS 492
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Match: SMESG000014782.1 (SMESG000014782.1)

HSP 1 Score: 161.384 bits (407), Expect = 3.358e-44
Identity = 103/318 (32.39%), Postives = 162/318 (50.94%), Query Frame = 3 
Query:  204 WRCEKRTGT--------ARVHTKDD-EIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAA-HLLPKRTTLARDVRRHRQKVGPNDQDDIMRYSLTQSNXXXXXXXXXXXXXXAAQDD----LIF--------LSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAYRDIEVICDYKEDNFI 1091
            + CE+R GT        A+V   +D  +V  +  H HT      E+ K RA +KR+ E T+ET + I+  EL  +   AA  ++P R  +  ++RR +QK        + R          +      +I  +  DD    LIF        +   +HWF D TF+V P  + QLYT+H  +   + P ++ LL ++T+  Y  L +EI + +  ++P +I  DFE AAI +    F    I GC FH  Q ++RK+Q+ G   RY+N+ DFAL ++++  LAFV  ++VI+ FE L E  +  +   + DY EDN+I
Sbjct:   38 YECERRRGTVKNTSECRAKVQLNEDLSVVSYLHRHTHTADSVHCEILKVRASIKRKAEETEETPKQILGQELQQLSQQAAVQMVPIRH-IRGNIRRVKQKKNAVHPVPVDRTFEILEEYKCLTDGEKFLIFDSGCDDPNRILIFGTERTLNLIKDSQHWFMDVTFKVVPELFFQLYTVHTLVTESIVPYLFVLLPNKTQITYCLLFEEIKVRQPQVHPTTITTDFERAAINSATECFANVEIHGCFFHLAQNIFRKVQSVGLQGRYQNDEDFALSIQKIAVLAFVLSENVIESFEKLQESLS-EEFATVMDYFEDNYI 353
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Match: SMESG000014782.1 (SMESG000014782.1)

HSP 1 Score: 161.384 bits (407), Expect = 3.446e-44
Identity = 103/318 (32.39%), Postives = 162/318 (50.94%), Query Frame = 3 
Query:  204 WRCEKRTGT--------ARVHTKDD-EIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVPVSAA-HLLPKRTTLARDVRRHRQKVGPNDQDDIMRYSLTQSNXXXXXXXXXXXXXXAAQDD----LIF--------LSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAYRDIEVICDYKEDNFI 1091
            + CE+R GT        A+V   +D  +V  +  H HT      E+ K RA +KR+ E T+ET + I+  EL  +   AA  ++P R  +  ++RR +QK        + R          +      +I  +  DD    LIF        +   +HWF D TF+V P  + QLYT+H  +   + P ++ LL ++T+  Y  L +EI + +  ++P +I  DFE AAI +    F    I GC FH  Q ++RK+Q+ G   RY+N+ DFAL ++++  LAFV  ++VI+ FE L E  +  +   + DY EDN+I
Sbjct:   40 YECERRRGTVKNTSECRAKVQLNEDLSVVSYLHRHTHTADSVHCEILKVRASIKRKAEETEETPKQILGQELQQLSQQAAVQMVPIRH-IRGNIRRVKQKKNAVHPVPVDRTFEILEEYKCLTDGEKFLIFDSGCDDPNRILIFGTERTLNLIKDSQHWFMDVTFKVVPELFFQLYTVHTLVTESIVPYLFVLLPNKTQITYCLLFEEIKVRQPQVHPTTITTDFERAAINSATECFANVEIHGCFFHLAQNIFRKVQSVGLQGRYQNDEDFALSIQKIAVLAFVLSENVIESFEKLQESLS-EEFATVMDYFEDNYI 355
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Match: SMESG000070712.1 (SMESG000070712.1)

HSP 1 Score: 122.865 bits (307), Expect = 7.786e-30
Identity = 61/162 (37.65%), Postives = 94/162 (58.02%), Query Frame = 3 
Query:  606 EHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAYRDIEVICDYKEDNFI 1091
            ++WF DGTF+V P  + QLYT+   + G    V+    + +T+  Y  L +EI +L+  ++PA+I  DFE AAI A    F    I GC FH  Q +++K Q+ G  +RY+N+ DFAL ++ + A AF P + VI+ FE L +     +   + DY E+N+I
Sbjct:   74 QNWFMDGTFKVVPELFFQLYTVKALVTGSNCSVLVFPPTKKTQIVYCRLFEEIKVLQPQVHPATITTDFERAAINAATECFANIKIHGCFFHNAQKIFKKFQSVGLQDRYQNDEDFALSIRMIAASAFFPIEKVIESFETL-QKSLSDEFATVMDYFEENYI 234
[Close]
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Match: SMESG000073500.1 (SMESG000073500.1)

HSP 1 Score: 125.561 bits (314), Expect = 1.123e-29
Identity = 78/270 (28.89%), Postives = 129/270 (47.78%), Query Frame = 3 
Query:  606 EHWFADGTFRVSPNGYDQLYTIHGFINGEVFPVVYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLI-----EDPAYRDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQYSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMVTDYNN 1400
            E  + DGTF   P+ + QLYT H  +     P +Y LL  +  + Y  + + +  L   L+P  ++VDF+ A + A    F  A + GC FH  Q + R++   GF   Y +  D  L++K L ALAFVP  +V+  F+ L      EDP       +  Y    +I            F +++W+Q+    +N P++ N  EG+HNA N     +HP+   L   LQ++   ++L     +      KKK+ Y  +++ + T V +Y++
Sbjct: 1520 ETIYGDGTFDKCPSMFYQLYTWHVQVGTSYPPCIYFLLQKKNRKTYIRMFEIMKQLLTNLSPRKVLVDFKKACMTAAAVAFLDAEVKGCYFHLCQSLVRRVNCVGFKNLYESNVDVKLMLKSLCALAFVPANEVMSAFDILARTFPDEDP----YNAVLSYFFQTYI---EGAIGRDSMFPVRIWNQWEAAAENSPKTTNCCEGFHNALNATFHCSHPSIWLLYDGLQRDIACHRLTLANYQ-TERPEKKKRKYESLHQGVATAVQEYDS 1781
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Match: SMESG000054573.1 (SMESG000054573.1)

HSP 1 Score: 121.709 bits (304), Expect = 2.829e-29
Identity = 66/205 (32.20%), Postives = 110/205 (53.66%), Query Frame = 3 
Query:  771 LKAGLNPASIMVDFELAAIRAFQSTFPTATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQDVIDLFEHLIEDPAYRDIEVICDYKEDNFIXXXXXXXXXXXXFSIQLWSQFSRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQYSNQLLRRQLELGTTAGKKKKTYIRVNEALHTMV 1385
            +K  L P +++ DFE AA+  F + F +  I G  FHF QC+ RK+Q  G++ RY N+PD+ + ++ L+A+A+V   D +  +E LI+  ++ +++ + +Y ED FI            F     +     +      NN++EG HNAF   V  AH T  KL  KLQ++Q +NQ+   ++  G     +KK Y  ++  LH ++
Sbjct:  281 IKPRLQPQTVITDFEAAAMNVFTNVF-SPQIHGYHFHFEQCISRKVQEYGYAGRYNNKPDYVMYIQMLVAMAYVSNNDKVSFYEELIDLDSFPEVDQLLNYFEDLFIEEDENDEEMVLFFHPIYGTATKLSVMTFLIHNNSMEGRHNAFARTVQIAHTTLPKLVVKLQKKQNNNQMNIERMLAGEVPPMRKKKYRDLDSQLHIVL 484
The following BLAST results are available for this feature:
BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F09E5.101.060e-830.71pep chromosome:WBcel235:II:5348096:5350546:-1 gene... [more]
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0C2J8U04.493e-8334.35Uncharacterized protein OS=Thelohanellus kitauei O... [more]
A0A0C2IYG67.331e-8334.35Uncharacterized protein OS=Thelohanellus kitauei O... [more]
A0A5N4B0X75.821e-8035.19Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A0C2IP162.992e-7833.92Uncharacterized protein OS=Thelohanellus kitauei O... [more]
A0A5N4APK11.533e-7735.74Uncharacterized protein OS=Photinus pyralis OX=705... [more]
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FLYWCH-type zinc finger-containing protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014782.13.358e-4432.39SMESG000014782.1[more]
SMESG000014782.13.446e-4432.39SMESG000014782.1[more]
SMESG000070712.17.786e-3037.65SMESG000070712.1[more]
SMESG000073500.11.123e-2928.89SMESG000073500.1[more]
SMESG000054573.12.829e-2932.20SMESG000054573.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026565 ID=SMED30026565|Name=FLYWCH-type zinc finger-containing protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1727bp
TTCATATTAGTTGGAAATATAATTTTATTTTACAATTTATTGCGTTTACT
GTAGACTATTATAGCTACTTAGTATTTACAAATAAATTTTGAAAAGTTAC
CACAATGCTAGAATTCGTTACATCACAGAAAGGAAATCGTAAGCTTATCG
TTCAAAGTTACTTGTTTACGAAACATTCGGAGAACAGTGACGGAAAAAGC
CTTTGGCGCTGTGAAAAAAGAACGGGCACAGCGCGAGTGCACACAAAAGA
TGATGAAATTGTTCATGAAGTTGCAACTCACAATCACACGGTAACGCATG
GTCAAGTAGAGGTCGAAAAAGCACGAGCCGGCATGAAAAGAAGAGGTGAA
ACTACTGACGAAACAACACGATCAATTGTTCAGAATGAATTGATGAATGT
TCCAGTTTCAGCAGCGCATCTACTTCCAAAACGAACAACATTGGCACGAG
ACGTACGACGTCATCGTCAGAAGGTGGGACCAAACGATCAAGATGACATC
ATGAGATATTCTTTGACGCAGAGTAACAGACTTTTCATCCGTATTAGGAG
AGACGACATGATCATCTTCGCAGCTCAGGATGATTTAATTTTCCTTTCCA
GATGTGAGCACTGGTTTGCAGATGGAACATTTCGAGTGAGTCCAAATGGA
TATGACCAACTATACACAATTCATGGCTTCATTAATGGCGAAGTTTTCCC
GGTAGTATATGCACTACTATCTTCAAGAACAGAAGAAGCATATCAACATC
TGCTCCAGGAAATTCTGATATTGAAAGCAGGTCTGAATCCAGCTTCAATT
ATGGTTGATTTCGAACTAGCAGCAATTAGAGCATTCCAGAGCACCTTCCC
GACAGCAACAATTACTGGATGCATGTTTCACTTCGGCCAATGTGTTTGGA
GGAAATTGCAAGCGGAAGGATTCTCAGAACGTTACAGAAATGAACCAGAT
TTCGCGCTTCTCGTCAAACGCCTACTTGCCTTAGCATTCGTCCCACCGCA
GGATGTTATTGATTTGTTTGAACATTTAATCGAAGATCCAGCTTATCGTG
ACATCGAGGTTATCTGTGATTACAAGGAAGATAATTTCATTGGTAGATTA
AGAAGGCGAAGAAGAGGACCACCAAGATTCTCAATTCAACTTTGGAGTCA
ATTTTCTAGAGTAATCGACAACCTGCCTCGCAGCAACAACGCAATCGAGG
GGTGGCATAATGCATTCAACAATGTTGTCGGTTTTGCTCATCCGACCACA
ACCAAACTTGCACGCAAACTACAACAAGAACAATACAGCAATCAACTTCT
TCGTCGTCAATTGGAACTGGGAACAACGGCTGGGAAGAAAAAGAAAACAT
ACATCCGCGTCAACGAGGCGTTGCACACTATGGTGACCGACTACAACAAC
CGAGATTCAATCACATATTTAGGAGACATTGCACGTGTTTTAAACATTAA
TGTTGTTTAACTTCTAATTGTTGTTTAATTTTAATTGCTAAAAGACTCGA
TTATTTATTTTTAGATGCGTTTTTAAAATAACCTAATAATGTTAATAATA
CTGTGCTTTTCATTAACTGAATATCTCAGACTATTATTGATATAGGCTAA
ATATTATTACAATAATAAATTACATTAGGACATAATTTTAATAAATATAT
TTTGTCCGTGACATTTTGTCCGCTGACATTTTGTCCCCATGACATTTTGT
CCTTGACATTTTGTCCGGCCACCATTT
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protein sequence of SMED30026565-orf-1

>SMED30026565-orf-1 ID=SMED30026565-orf-1|Name=SMED30026565-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=452bp
MLEFVTSQKGNRKLIVQSYLFTKHSENSDGKSLWRCEKRTGTARVHTKDD
EIVHEVATHNHTVTHGQVEVEKARAGMKRRGETTDETTRSIVQNELMNVP
VSAAHLLPKRTTLARDVRRHRQKVGPNDQDDIMRYSLTQSNRLFIRIRRD
DMIIFAAQDDLIFLSRCEHWFADGTFRVSPNGYDQLYTIHGFINGEVFPV
VYALLSSRTEEAYQHLLQEILILKAGLNPASIMVDFELAAIRAFQSTFPT
ATITGCMFHFGQCVWRKLQAEGFSERYRNEPDFALLVKRLLALAFVPPQD
VIDLFEHLIEDPAYRDIEVICDYKEDNFIGRLRRRRRGPPRFSIQLWSQF
SRVIDNLPRSNNAIEGWHNAFNNVVGFAHPTTTKLARKLQQEQYSNQLLR
RQLELGTTAGKKKKTYIRVNEALHTMVTDYNNRDSITYLGDIARVLNINV
V*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR007588Znf_FLYWCH
IPR018289MULE_transposase_dom