SMED30026550
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026550 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30026550 aligns in the following genomic locations:
Homology
BLAST of SMED30026550 vs. TrEMBL
Match: A0A0K0EJP9 (HTH_48 domain-containing protein OS=Strongyloides stercoralis OX=6248 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 8.734e-11 Identity = 41/109 (37.61%), Postives = 61/109 (55.96%), Query Frame = -1 Query: 874 AVTVCQEMNPTYGDVLKINKYQSWLRKFTCDDFDLSLGERKNRSSKLDAGLLSSLVKSDSRQSIKDLSDKLNSSL*TIYERLKKIVKVDRYEILLPQNLCENNKAQQRA 1200 A T + + Y + L + K Q W KF DFDLS R R +D +L ++V+++ Q+IK+LS KLNS TI E +K+I KV+R + + NL E NK + + Sbjct: 25 ATTATENICDAYPEALNVRKCQRWFVKFRSGDFDLSDASRSGRPITVDNDILQAIVEANPYQTIKELSVKLNSPWSTIQEHMKQIGKVNREGVWVSDNLSEENKVNRSS 133
BLAST of SMED30026550 vs. TrEMBL
Match: A0A0K0E0J2 (HTH_48 domain-containing protein OS=Strongyloides stercoralis OX=6248 PE=4 SV=1) HSP 1 Score: 69.707 bits (169), Expect = 3.122e-10 Identity = 41/107 (38.32%), Postives = 62/107 (57.94%), Query Frame = -1 Query: 880 AVTVCQEMNPTYGDVLKINKYQSWLRKFTCDDFDLSLGERKNRSSKLDAGLLSSLVKSDSRQSIKDLSDKLNSSL*TIYERLKKIVKVDRYEILLPQNLCENNKAQQ 1200 A T + + Y L + K Q W KF DFDLS R R+S L+ +L +++D Q+I++LS+KLNS+ T+ + LK+I KV + +P NL E NKA++ Sbjct: 17 ATTATKNICSVYPGFLDVRKCQRWFLKFKSGDFDLSDANRSGRTSALNNDIL---LEADLCQTIEELSNKLNSTCSTVQKHLKQIGKVYSEGVWVPHNLSEENKAKR 120
BLAST of SMED30026550 vs. TrEMBL
Match: T2MC65 (Histone-lysine N-methyltransferase SETMAR (Fragment) OS=Hydra vulgaris OX=6087 GN=SETMAR PE=2 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 8.953e-9 Identity = 41/126 (32.54%), Postives = 61/126 (48.41%), Query Frame = -1 Query: 856 AVTVCQEMNPTYGDVLKINKYQSWLRKFTCDDFDLSLGERKNRSSKLDAGLLSSLVKSDSRQSIKDLSDKLNSSL*TIYERLKKIVKVDRYEILLPQNLCENNKA-----------QQRAQIFLSR 1200 A + ++ Y L + K Q W KF +FDLS R R LD +L + V+++ Q+I++LS+ LN TI E L +I K+ R + +P NL NKA + A+ FL R Sbjct: 24 ATVATKNISDVYPGSLDVRKCQRWFSKFRSGNFDLSDAHRPGRPMSLDNDVLRAEVEANPCQTIEELSNTLNQPWSTIQEHLHQIGKISRAGVWVPHNLSVQNKANRSTTCNALIQRHHAEPFLDR 149
BLAST of SMED30026550 vs. TrEMBL
Match: F4WXF5 (Histone-lysine N-methyltransferase SETMAR OS=Acromyrmex echinatior OX=103372 GN=G5I_10640 PE=4 SV=1) HSP 1 Score: 62.7734 bits (151), Expect = 4.469e-8 Identity = 34/105 (32.38%), Postives = 58/105 (55.24%), Query Frame = -1 Query: 889 AVTVCQEMNPTYGDVLKINK-YQSWLRKFTCDDFDLSLGERKNRSSKLDAGLLSSLVKSDSRQSIKDLSDKLNSSL*TIYERLKKIVKVDRYEILLPQNLCENNK 1200 A +++ YG+ L ++ + W KF D L G R R S LD LL ++++ + RQS +D++ +LN+S T+ L+K+ K+ + + +P NL E NK Sbjct: 22 AKGTAEKICSVYGEGLISDRTVRHWFAKFRSGDTTLKDGPRAGRPSDLDDKLLKAMLEQNPRQSTRDIAGRLNTSQSTVCRHLEKLGKISKLGVWVPHNLSERNK 126
BLAST of SMED30026550 vs. TrEMBL
Match: A0A154PQM4 (Histone-lysine N-methyltransferase SETMAR OS=Dufourea novaeangliae OX=178035 GN=WN55_06632 PE=4 SV=1) HSP 1 Score: 64.6994 bits (156), Expect = 5.188e-8 Identity = 38/107 (35.51%), Postives = 60/107 (56.07%), Query Frame = -1 Query: 886 AVTVCQEMNPTYG-DVLKINKYQSWLRKFTCDDFDLSLGERKNRSSKLDAG-LLSSLVKSDSRQSIKDLSDKLNSSL*TIYERLKKIVKVDRYEILLPQNLCENNKA 1200 A +E+ YG D + Q+W +KF DF L +R R +++D +L + V+++ Q+I+DLS+ LN TI+E L++I KV R + +P NL NKA Sbjct: 22 AAEAHKEICEVYGVDCITDRTCQNWFKKFRSGDFSLKDDQRSGRPTEVDDNDVLRAEVEANPCQTIEDLSNTLNQPWSTIHEHLQEIRKVSRAGVWVPHNLSGENKA 128
BLAST of SMED30026550 vs. Planmine SMEST
Match: SMESG000078337.1 (SMESG000078337.1) HSP 1 Score: 429.869 bits (1104), Expect = 2.854e-144 Identity = 210/216 (97.22%), Postives = 210/216 (97.22%), Query Frame = 3 Query: 36 MSCFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRKRNYASRLVCVC 683 MS FTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRKRNYAS C Sbjct: 1 MSYFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRKRNYASSDFVTC 216
BLAST of SMED30026550 vs. Planmine SMEST
Match: SMESG000078337.1 (SMESG000078337.1) HSP 1 Score: 412.535 bits (1059), Expect = 2.646e-142 Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 3 Query: 36 MSCFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRK 650 MS FTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRK Sbjct: 1 MSYFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRK 205
BLAST of SMED30026550 vs. Planmine SMEST
Match: SMESG000078337.1 (SMESG000078337.1) HSP 1 Score: 419.853 bits (1078), Expect = 2.081e-140 Identity = 204/210 (97.14%), Postives = 207/210 (98.57%), Query Frame = 3 Query: 36 MSCFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRKRNYAS 665 MS FTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRK ++ + Sbjct: 1 MSYFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTLEQFLNSLTQQNDKIFFTDFIEGIKKLTNSDSKITDNELDNESNCDYHQIFANNDSSPFINDGDNYSTGDSGLDNESGINRENVCNGIGKQNRKRIKAFQNADLSQSIETLKRRFSENESLNNTDYDGQIYKTNQTYHNIPHISLKSSYNCERRKSDFVT 210 The following BLAST results are available for this feature:
BLAST of SMED30026550 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30026550 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30026550 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30026550 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30026550 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30026550 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026550 ID=SMED30026550|Name=SMED30026550|organism=Schmidtea mediterranea sexual|type=transcript|length=1899bpback to top protein sequence of SMED30026550-orf-1 >SMED30026550-orf-1 ID=SMED30026550-orf-1|Name=SMED30026550-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp MSCFTEVFHALDHDNKGYILFDDFKNEMLKFLSTGEQSFLTKEKLDQKKTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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