Fumarate hydratase, class I

Overview
NameFumarate hydratase, class I
Smed IDSMED30026457
Length (bp)1830
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Fumarate hydratase, class I (SMED30026457) t-SNE clustered cells

Violin plots show distribution of expression levels for Fumarate hydratase, class I (SMED30026457) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Fumarate hydratase, class I (SMED30026457) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Fumarate hydratase, class I (SMED30026457) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026457

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30026457SMESG000075499.1 SMESG000009591.1 dd_Smed_v4_2405_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026457SMESG000075499.1 SMESG000009591.1 dd_Smed_v4_2405_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026457SMESG000075499.1 SMESG000009591.1 dd_Smed_v4_2405_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026457SMESG000075499.1 SMESG000009591.1 dd_Smed_v4_2405_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Match: sp|Q8X4P8|FUMA_ECO57 (Fumarate hydratase class I, aerobic OS=Escherichia coli O157:H7 OX=83334 GN=fumA PE=3 SV=3)

HSP 1 Score: 503.827 bits (1296), Expect = 0.000e+0
Identity = 234/328 (71.34%), Postives = 275/328 (83.84%), Query Frame = 1 
Query:  781 MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQV 1764
            ++K++TLGTAACPPYH+A VIGGTSAET LKTVKLAS KY D L T GN HG+AFRD+ELE ++L   +++G+GAQFGGKYF HD+RV+RLPRHGASCPVG+GVSCSADR I  KI R G+++EKLE +P KY+PE        + V+VDLNRPMKE+L  LS+YP+ TRLSL+GT+IV RDIAHAKL ER++ G+ LP YI  HP+YYAGPAKTP G  SGS GPTTAGRMDSYV Q Q  GGSMIMLAKGNRS+QVTDACKK+GGFYLGSIGGPAAVLAQ  IK +E VEY ELGMEAIW++EVE+FPAFI+VDDKGNDFFQ+ Q+
Sbjct:  213 VEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL 540

HSP 2 Score: 266.159 bits (679), Expect = 0.000e+0
Identity = 133/220 (60.45%), Postives = 166/220 (75.45%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            KPF++Q  F ++K    D E   LT ++V + +F  +EILKV  +ALT+LA +A  D + +LRPAH + ++ IL DPE+S+NDK+VAL+ L+N+ IAA  VLP CQDTGTAII+GKKGQ VWT G D  AL+RGVYNTY   NLRYSQ AP+ MY E NTGTNLPAQI++YA DGDEYKFL IAKGGGSANKT+LYQ+TKALL P KL  ++ EK   +G
Sbjct:    4 KPFHYQAPFPLKK---DDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLG 220
[Close]
BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Match: sp|P0AC33|FUMA_ECOLI (Fumarate hydratase class I, aerobic OS=Escherichia coli (strain K12) OX=83333 GN=fumA PE=1 SV=2)

HSP 1 Score: 503.442 bits (1295), Expect = 0.000e+0
Identity = 234/328 (71.34%), Postives = 275/328 (83.84%), Query Frame = 1 
Query:  781 MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQV 1764
            ++K++TLGTAACPPYH+A VIGGTSAET LKTVKLAS KY D L T GN HG+AFRD+ELE ++L   +++G+GAQFGGKYF HD+RV+RLPRHGASCPVG+GVSCSADR I  KI R G+++EKLE +P KY+PE        + V+VDLNRPMKE+L  LS+YP+ TRLSL+GT+IV RDIAHAKL ER++ G+ LP YI  HP+YYAGPAKTP G  SGS GPTTAGRMDSYV Q Q  GGSMIMLAKGNRS+QVTDACKK+GGFYLGSIGGPAAVLAQ  IK +E VEY ELGMEAIW++EVE+FPAFI+VDDKGNDFFQ+ Q+
Sbjct:  213 VEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL 540

HSP 2 Score: 266.544 bits (680), Expect = 0.000e+0
Identity = 133/220 (60.45%), Postives = 166/220 (75.45%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            KPF++Q  F ++K    D E   LT ++V + +F  +EILKV  +ALT+LA +A  D + +LRPAH + ++ IL DPE+S+NDK+VAL+ L+N+ IAA  VLP CQDTGTAII+GKKGQ VWT G D  AL+RGVYNTY   NLRYSQ AP+ MY E NTGTNLPAQI++YA DGDEYKFL IAKGGGSANKT+LYQ+TKALL P KL  ++ EK   +G
Sbjct:    4 KPFHYQAPFPLKK---DDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLG 220
[Close]
BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Match: sp|P0AC34|FUMA_ECOL6 (Fumarate hydratase class I, aerobic OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=fumA PE=3 SV=2)

HSP 1 Score: 503.442 bits (1295), Expect = 0.000e+0
Identity = 234/328 (71.34%), Postives = 275/328 (83.84%), Query Frame = 1 
Query:  781 MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQV 1764
            ++K++TLGTAACPPYH+A VIGGTSAET LKTVKLAS KY D L T GN HG+AFRD+ELE ++L   +++G+GAQFGGKYF HD+RV+RLPRHGASCPVG+GVSCSADR I  KI R G+++EKLE +P KY+PE        + V+VDLNRPMKE+L  LS+YP+ TRLSL+GT+IV RDIAHAKL ER++ G+ LP YI  HP+YYAGPAKTP G  SGS GPTTAGRMDSYV Q Q  GGSMIMLAKGNRS+QVTDACKK+GGFYLGSIGGPAAVLAQ  IK +E VEY ELGMEAIW++EVE+FPAFI+VDDKGNDFFQ+ Q+
Sbjct:  213 VEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL 540

HSP 2 Score: 266.544 bits (680), Expect = 0.000e+0
Identity = 133/220 (60.45%), Postives = 166/220 (75.45%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            KPF++Q  F ++K    D E   LT ++V + +F  +EILKV  +ALT+LA +A  D + +LRPAH + ++ IL DPE+S+NDK+VAL+ L+N+ IAA  VLP CQDTGTAII+GKKGQ VWT G D  AL+RGVYNTY   NLRYSQ AP+ MY E NTGTNLPAQI++YA DGDEYKFL IAKGGGSANKT+LYQ+TKALL P KL  ++ EK   +G
Sbjct:    4 KPFHYQAPFPLKK---DDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLG 220
[Close]
BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Match: sp|P40720|FUMA_SALTY (Fumarate hydratase class I, aerobic OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=fumA PE=3 SV=3)

HSP 1 Score: 492.271 bits (1266), Expect = 0.000e+0
Identity = 233/327 (71.25%), Postives = 275/327 (84.10%), Query Frame = 1 
Query:  781 MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQ 1761
            + K++TLGTAACPPYH+A VIGGTSAE  LKTVKLAS KY D L T GN HG+AFRD+ELE ++L   +++G+GAQFGGKYF HD+RV+RLPRHGASCPVG+GVSCSADR I  KI R+G+++EKLER+P KY+PE        + V+VDLNRPM E+L  LS+YP+ TRLSL+GT+IV RDIAHAKL ER+++G+ LP YI  HP+YYAGPAKTP G  SGS GPTTAGRMDSYV Q Q  GGSMIMLAKGNRS+QVTDACKK+GGFYLGSIGGPAAVLAQ  IK++E VEY ELGMEAIW++EVE+FPAFI+VDDKGNDFFQ+ Q
Sbjct:  213 VDKMRTLGTAACPPYHIAFVIGGTSAEANLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQ 539

HSP 2 Score: 263.077 bits (671), Expect = 0.000e+0
Identity = 130/220 (59.09%), Postives = 166/220 (75.45%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            KPF++QD F ++K    D E   LT ++V + +F  +EILKV  +ALT+LA +A  D + +LRPAH + ++ IL DP++S+NDK+VAL+ L+N+ IAA  VLP CQDTGTAII+GKKGQ VWT G D  AL+RGVYNTY   NLRYSQ A + MY E NTGTNLPAQI++Y+ DGDEYKFL IAKGGGSANKT+LYQ+TKALL P KL  ++ +K   +G
Sbjct:    4 KPFFYQDPFPLKK---DDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLG 220
[Close]
BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Match: sp|P14407|FUMB_ECOLI (Fumarate hydratase class I, anaerobic OS=Escherichia coli (strain K12) OX=83333 GN=fumB PE=1 SV=2)

HSP 1 Score: 490.73 bits (1262), Expect = 0.000e+0
Identity = 227/325 (69.85%), Postives = 268/325 (82.46%), Query Frame = 1 
Query:  781 MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQE 1755
            ++K++TLGTAACPPYH+A VIGGTSAET LKTVKLAS  Y D L T GN HG+AFRD++LE ++L   + +G+GAQFGGKYF HD+RV+RLPRHGASCPVG+GVSCSADR I  KI R G+++EKLE +P +Y+P+        + VKVDLNRPMKE+L  LS+YP+ TRLSL+GT+IV RDIAHAKL E ++ G+ LP YI  HP+YYAGPAKTP G PSGS GPTTAGRMDSYV   Q  GGSMIMLAKGNRS+QVTDAC K+GGFYLGSIGGPAAVLAQ  IK +E V Y ELGMEAIW++EVE+FPAFI+VDDKGNDFFQ+
Sbjct:  213 VEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQ 537

HSP 2 Score: 263.077 bits (671), Expect = 0.000e+0
Identity = 133/220 (60.45%), Postives = 163/220 (74.09%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            KPF +Q  F + K    + E   LT DYV +  F  + ILKV+ +ALT+LA++A  D + +LRPAH K ++ IL DPE+S+NDK+VAL+ L+N+ IAA  VLP CQDTGTAII+GKKGQ VWT G D E LS+GVYNTY   NLRYSQ A + MY E NTGTNLPAQI++YA DGDEYKFL +AKGGGSANKT+LYQ+TKALL P KL  F+ EK   +G
Sbjct:    4 KPFIYQAPFPMGK---DNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEETLSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLG 220
[Close]
BLAST of Fumarate hydratase, class I vs. TrEMBL
Match: A0A433TL16 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_010036 PE=4 SV=1)

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 271/387 (70.03%), Postives = 318/387 (82.17%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSYLLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I   ++  TP  ++  +   + K  G   K F   + K             +KIKTLGTAACPPYHLA+VIGGTSAE TLKTVKLASTKYLDNL T+GN HGRAFRDL+LE QI  +T+ +GIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKIT  G+FLE+LE +P +Y+PE+ D +L  +VV+V+LN+ M  VL  L ++PI+TRLSL+GTL+VARDIAHAKL E +++G+ LP Y+  HPVYYAGPAKTP G+PSGSFGPTTAGRMDSYVA+FQK GGSM+MLAKGNRSKQVT ACK+YGGFYLGSIGGPAA+LAQNCIKKVEV+E+ ELGMEAIWRVEVENFPAFIVVD+KGNDFF+EWQVE
Sbjct:  196 TRTNLPAQI---EIYATP--SSQYDFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKIKTLGTAACPPYHLALVIGGTSAEFTLKTVKLASTKYLDNLPTTGNEHGRAFRDLDLEKQIFEMTQQIGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITEEGVFLEQLETNPGQYLPEVEDKHLGGNVVQVNLNQGMSGVLKQLDQHPIRTRLSLTGTLVVARDIAHAKLKEGLDRGEGLPQYLKDHPVYYAGPAKTPEGLPSGSFGPTTAGRMDSYVAEFQKAGGSMVMLAKGNRSKQVTAACKQYGGFYLGSIGGPAAILAQNCIKKVEVLEFAELGMEAIWRVEVENFPAFIVVDNKGNDFFKEWQVE 577

HSP 2 Score: 323.939 bits (829), Expect = 2.337e-99
Identity = 160/237 (67.51%), Postives = 188/237 (79.32%), Query Frame = 3 
Query:   93 KIIRAPAKYVRFYCANAKPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            +++ A AK       + KPF +QDI E++    HDV  +KLT D+V       +++L V  +ALT+LAE+AMIDIAHLLRPAHL+ L+ IL+DP++S NDKFVALELLKNA++AA MVLPGCQDTGTAI MGKKGQ VWT+G DAEA+SRG+YNTY  KNLRYSQVAP+ MY E NT TNLPAQIEIYAT   +Y FLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK   +G
Sbjct:   24 RVVPAAAKATS--APDVKPFQYQDILELQS--KHDVPYKKLTSDFVSTIDVKGRKVLTVQPEALTMLAEQAMIDIAHLLRPAHLQQLAKILTDPDASSNDKFVALELLKNANVAAGMVLPGCQDTGTAICMGKKGQYVWTEGDDAEAISRGIYNTYVKKNLRYSQVAPLDMYTETNTRTNLPAQIEIYATPSSQYDFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKIKTLG 256
[Close]
BLAST of Fumarate hydratase, class I vs. TrEMBL
Match: G4VB23 (Pol-related OS=Schistosoma mansoni OX=6183 GN=Smp_126020 PE=4 SV=1)

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 274/387 (70.80%), Postives = 315/387 (81.40%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSYLLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I +          +  N   + K  G   K F   + K      + +    +KIKTLGTAACPPYHLAVVIGGTSAE TLKTVKLAS KYLD+L ++GN HGRAFRD E E +IL LTR VGIGAQFGGKYFCHDVRV+RLPRHGASCPVGIGVSCSADRQILG+IT +G+FLE+LE DPSKY+P+ + + L  DVVKV+LN PMKE+L  LS+YPIKTRLSL+GTL+VARDIAHAK+ ER++ GQ +PDY+  HPVYYAGPAKTP   PSGSFGPTTAGRMDSYV QFQ+ GGSMIMLAKGNRSK VT+ACKK+GGFYLGSIGGPAA+LAQNCIKKVEV+EY ELGMEAIWRVEV +FPAFIV+DDKGNDFF+EWQVE
Sbjct:  189 TCTNLPAQIEIYSTS-----GSEYNFLFVAKGGGSANKTFLYQQTKALLNPSSLYSFVNEKIKTLGTAACPPYHLAVVIGGTSAEFTLKTVKLASCKYLDHLPSTGNEHGRAFRDREAEEKILELTRKVGIGAQFGGKYFCHDVRVIRLPRHGASCPVGIGVSCSADRQILGRITNDGVFLEQLETDPSKYLPDPSLNQLPNDVVKVNLNLPMKEILAELSRYPIKTRLSLTGTLVVARDIAHAKIKERLDSGQPMPDYLRNHPVYYAGPAKTPMNYPSGSFGPTTAGRMDSYVDQFQQAGGSMIMLAKGNRSKAVTNACKKHGGFYLGSIGGPAAILAQNCIKKVEVLEYPELGMEAIWRVEVVDFPAFIVIDDKGNDFFKEWQVE 570

HSP 2 Score: 333.569 bits (854), Expect = 3.288e-103
Identity = 155/215 (72.09%), Postives = 183/215 (85.12%), Query Frame = 3 
Query:  144 KPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            KPF++QDI ++EK PY +    KLTGD V + +F N  +LKVD+ ALT+L+E+AMID+AHLLRPAHL+SLS IL DPE+S NDKFVALELLKNA++A  MVLPGCQDTGTA+ MGKKGQ VWTDG DAE++SRG+YNTYTSK LRYSQVAP+ ++ E+NT TNLPAQIEIY+T G EY FLF+AKGGGSANKTFLYQQTKALLNP  L +FVNEK
Sbjct:   30 KPFFYQDILQLEKPPYAETPFRKLTGDLVSLREFDNIRLLKVDSMALTLLSEQAMIDVAHLLRPAHLQSLSNILKDPEASNNDKFVALELLKNANVACGMVLPGCQDTGTAVCMGKKGQFVWTDGNDAESISRGIYNTYTSKYLRYSQVAPLDLFTEKNTCTNLPAQIEIYSTSGSEYNFLFVAKGGGSANKTFLYQQTKALLNPSSLYSFVNEK 244
[Close]
BLAST of Fumarate hydratase, class I vs. TrEMBL
Match: V4A1L2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_123266 PE=4 SV=1)

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 273/390 (70.00%), Postives = 316/390 (81.03%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSY---LLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I +         AT++N Y    + K  G   K F   + K             +KIKTLGTAACPPYHLA V+GGTSAE  LKTVKLASTKYLDNL T+GN HGRAFRDLELE +I  LT+ +GIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQ  GKIT  G+FLE+LE +P +Y+PE+ D +L  +V+KV+LN+PMK+VL LLS+YPIKTRLSLSGTL+VARDIAHAK+ ER+ KG+ +P Y+  HPVYYAGPAKTP G+ SGSFGPTTAGRMDSYV +FQ  GGSMIMLAKGNRSKQVT ACK++GGFYLGSIGGPAA+LAQNCIKKVEV+EY ELGMEAIWRVEVE+FPAF+VVD+KGNDFF+EWQVE
Sbjct:  140 TKTNLPAQIEI--------YATDSNQYDFLFIAKGGGSANKTFLYQQTKALLNPEKLLTFVNEKIKTLGTAACPPYHLAFVVGGTSAEFNLKTVKLASTKYLDNLPTTGNEHGRAFRDLELEDEIHKLTQVMGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQAHGKITCEGVFLEQLESNPGQYLPEVEDKDLGGEVIKVNLNQPMKDVLNLLSQYPIKTRLSLSGTLVVARDIAHAKIKERLEKGEGMPQYLKDHPVYYAGPAKTPEGLASGSFGPTTAGRMDSYVDEFQSAGGSMIMLAKGNRSKQVTKACKQHGGFYLGSIGGPAAILAQNCIKKVEVLEYPELGMEAIWRVEVEDFPAFVVVDNKGNDFFKEWQVE 521

HSP 2 Score: 305.449 bits (781), Expect = 6.812e-93
Identity = 145/195 (74.36%), Postives = 166/195 (85.13%), Query Frame = 3 
Query:  204 MEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            M  ++GD+V       K++LK+  +ALT+LAE AMIDIAHLLRP HL+ LS IL DP +S ND+FVALELLKNA++AA MVLPGCQDTGTAI MGKKGQ VWT+G+D EA+SRG+YNTYT KNLRYSQVAP++MY E NT TNLPAQIEIYATD ++Y FLFIAKGGGSANKTFLYQQTKALLNPEKLL FVNEK
Sbjct:    1 MGYISGDFVSTFDVNGKQVLKIQPEALTLLAEHAMIDIAHLLRPKHLQQLSKILKDPSASSNDRFVALELLKNANVAAGMVLPGCQDTGTAICMGKKGQYVWTEGRDEEAISRGIYNTYTKKNLRYSQVAPISMYEEVNTKTNLPAQIEIYATDSNQYDFLFIAKGGGSANKTFLYQQTKALLNPEKLLTFVNEK 195
[Close]
BLAST of Fumarate hydratase, class I vs. TrEMBL
Match: A0A5K4ELK1 (Pol-related OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 274/389 (70.44%), Postives = 316/389 (81.23%), Query Frame = 1 
Query:  601 RCTMSRIPARICLLKLRFTPQMATNTNSYLLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            + T + +PA+I +          +  N   + K  G   K F   + K      + +    +KIKTLGTAACPPYHLAVVIGGTSAE TLKTVKLAS KYLD+L ++GN HGRAFRD E E +IL LTR VGIGAQFGGKYFCHDVRV+RLPRHGASCPVGIGVSCSADRQILG+IT +G+FLE+LE DPSKY+P+ + + L  DVVKV+LN PMKE+L  LS+YPIKTRLSL+GTL+VARDIAHAK+ ER++ GQ +PDY+  HPVYYAGPAKTP   PSGSFGPTTAGRMDSYV QFQ+ GGSMIMLAKGNRSK VT+ACKK+GGFYLGSIGGPAA+LAQNCIKKVEV+EY ELGMEAIWRVEV +FPAFIV+DDKGNDFF+EWQVE
Sbjct:   34 KNTCTNLPAQIEIYSTS-----GSEYNFLFVAKGGGSANKTFLYQQTKALLNPSSLYSFVNEKIKTLGTAACPPYHLAVVIGGTSAEFTLKTVKLASCKYLDHLPSTGNEHGRAFRDREAEEKILELTRKVGIGAQFGGKYFCHDVRVIRLPRHGASCPVGIGVSCSADRQILGRITNDGVFLEQLETDPSKYLPDPSLNQLPNDVVKVNLNLPMKEILAELSRYPIKTRLSLTGTLVVARDIAHAKIKERLDSGQPMPDYLRNHPVYYAGPAKTPMNYPSGSFGPTTAGRMDSYVDQFQQAGGSMIMLAKGNRSKAVTNACKKHGGFYLGSIGGPAAILAQNCIKKVEVLEYPELGMEAIWRVEVVDFPAFIVIDDKGNDFFKEWQVE 417

HSP 2 Score: 111.309 bits (277), Expect = 2.017e-22
Identity = 53/81 (65.43%), Postives = 64/81 (79.01%), Query Frame = 3 
Query:  546 VYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            + +T  S  + +  VAP+ ++ E+NT TNLPAQIEIY+T G EY FLF+AKGGGSANKTFLYQQTKALLNP  L +FVNEK
Sbjct:   11 IIHTLLSIFVTHKYVAPLDLFTEKNTCTNLPAQIEIYSTSGSEYNFLFVAKGGGSANKTFLYQQTKALLNPSSLYSFVNEK 91
[Close]
BLAST of Fumarate hydratase, class I vs. TrEMBL
Match: A0A5J4NLT4 (Fumarate hydratase, class I OS=Paragonimus westermani OX=34504 GN=DEA37_0005915 PE=4 SV=1)

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 273/390 (70.00%), Postives = 318/390 (81.54%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSY---LLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I +         +T  N Y    + K  G   K F   + K        +    +KIKTLGTAACPPYHLA+V+GGTSAE TLKTVKLAS KYLD L T+GN HGRAFRDL+ EA++L LTR VGIGAQFGGKYFCHDVRV+RLPRHGASCPVGIGVSCSADRQILG+IT +G+FLE+LE DPSKY+PE  +++LS +VVK++LNRPM +VL  LS++ I+TRLSL+GTL+VARDIAHAK+ ER++ G+ +P Y+  HPVYYAGPAKTPPG  SGSFGPTTAGRMDSYV QFQ  GGS IMLAKGNRS+QVT+ACKK+GGFYLGSIGGPAA+LAQ+CIKKVEV+EY ELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE
Sbjct:  186 TGTNLPAQIEI--------YSTEGNEYSFLFIAKGGGSANKTFLYQQTKALLNPDKLYSFVNEKIKTLGTAACPPYHLALVVGGTSAEMTLKTVKLASCKYLDYLPTTGNEHGRAFRDLDAEARVLQLTRKVGIGAQFGGKYFCHDVRVIRLPRHGASCPVGIGVSCSADRQILGRITSDGVFLEQLETDPSKYLPEPGEAHLSGEVVKINLNRPMDQVLSELSRHSIRTRLSLTGTLVVARDIAHAKIKERLDAGEPMPRYLCDHPVYYAGPAKTPPGFASGSFGPTTAGRMDSYVEQFQAAGGSKIMLAKGNRSRQVTNACKKFGGFYLGSIGGPAAILAQDCIKKVEVLEYPELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 567

HSP 2 Score: 324.709 bits (831), Expect = 7.475e-100
Identity = 154/227 (67.84%), Postives = 188/227 (82.82%), Query Frame = 3 
Query:  123 RFYCANAKPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKN*NIG 803
            R    + KPF +QDIFE  K  Y DV  +K++GDYV   +   ++IL+V ++AL  LA +AM+D++HLLRP+HL+ L+ IL D E+S NDKFVALELLKNA++AA MVLPGCQDTGTAI MGKKGQ VWTDG DAEA+SRGVY+TYT++NLRYSQVAP+ M+ E+NTGTNLPAQIEIY+T+G+EY FLFIAKGGGSANKTFLYQQTKALLNP+KL +FVNEK   +G
Sbjct:   20 RLATQSVKPFVYQDIFEATKPVYKDVPFKKISGDYVSTLEAAGQKILRVKSEALQSLASQAMVDVSHLLRPSHLQFLANILKDNEASSNDKFVALELLKNANVAAGMVLPGCQDTGTAICMGKKGQYVWTDGNDAEAISRGVYDTYTNRNLRYSQVAPLDMFTEKNTGTNLPAQIEIYSTEGNEYSFLFIAKGGGSANKTFLYQQTKALLNPDKLYSFVNEKIKTLG 246
[Close]
BLAST of Fumarate hydratase, class I vs. Planmine SMEST
Match: SMESG000009591.1 (SMESG000009591.1)

HSP 1 Score: 684.871 bits (1766), Expect = 0.000e+0
Identity = 339/390 (86.92%), Postives = 348/390 (89.23%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSY---LLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I +         AT+ + Y    + K  G   K F   + K             +KIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE
Sbjct:  126 TGTNLPAQIEI--------YATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 507

HSP 2 Score: 370.548 bits (950), Expect = 7.323e-122
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 3 
Query:  246 MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK
Sbjct:    1 MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 181
[Close]
BLAST of Fumarate hydratase, class I vs. Planmine SMEST
Match: SMESG000009591.1 (SMESG000009591.1)

HSP 1 Score: 684.1 bits (1764), Expect = 0.000e+0
Identity = 339/390 (86.92%), Postives = 348/390 (89.23%), Query Frame = 1 
Query:  607 TMSRIPARICLLKLRFTPQMATNTNSY---LLPKAAGRLIKRFSINRQKLC*IQRNCWLLSMKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 1767
            T + +PA+I +         AT+ + Y    + K  G   K F   + K             +KIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE
Sbjct:  182 TGTNLPAQIEI--------YATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVDLNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPDYILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLAKGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEAIWRVEVENFPAFIVVDDKGNDFFQEWQVE 563

HSP 2 Score: 491.886 bits (1265), Expect = 2.067e-168
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 3 
Query:   78 MNLLNKIIRAPAKYVRFYCANAKPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            MNLLNKIIRAPAKYVRFYCANAKPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK
Sbjct:    1 MNLLNKIIRAPAKYVRFYCANAKPFYFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 237
[Close]
BLAST of Fumarate hydratase, class I vs. Planmine SMEST
Match: SMESG000075499.1 (SMESG000075499.1)

HSP 1 Score: 371.703 bits (953), Expect = 0.000e+0
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 3 
Query:  246 MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 788
            MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK
Sbjct:    1 MNKEILKVDNKALTILAERAMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLPGCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVTMYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALLNPEKLLAFVNEK 181

HSP 2 Score: 231.491 bits (589), Expect = 0.000e+0
Identity = 111/115 (96.52%), Postives = 113/115 (98.26%), Query Frame = 1 
Query:  784 KKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQILGKI 1128
            +KIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQI  K+
Sbjct:  180 EKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNAHGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQISEKL 294

HSP 3 Score: 78.1814 bits (191), Expect = 0.000e+0
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 2 
Query: 1118 SEKLPVTDCSWRNWNAIPANTCPRSPIQISLRTW 1219
            SEKLPVTDCSWRNWNAIPANTCPRSPIQISLRTW
Sbjct:  291 SEKLPVTDCSWRNWNAIPANTCPRSPIQISLRTW 324
The following BLAST results are available for this feature:
BLAST of Fumarate hydratase, class I vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8X4P8|FUMA_ECO570.000e+071.34Fumarate hydratase class I, aerobic OS=Escherichia... [more]
sp|P0AC33|FUMA_ECOLI0.000e+071.34Fumarate hydratase class I, aerobic OS=Escherichia... [more]
sp|P0AC34|FUMA_ECOL60.000e+071.34Fumarate hydratase class I, aerobic OS=Escherichia... [more]
sp|P40720|FUMA_SALTY0.000e+071.25Fumarate hydratase class I, aerobic OS=Salmonella ... [more]
sp|P14407|FUMB_ECOLI0.000e+069.85Fumarate hydratase class I, anaerobic OS=Escherich... [more]
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BLAST of Fumarate hydratase, class I vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A433TL162.337e-9970.03Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
G4VB233.288e-10370.80Pol-related OS=Schistosoma mansoni OX=6183 GN=Smp_... [more]
V4A1L26.812e-9370.00Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A5K4ELK12.017e-2270.44Pol-related OS=Schistosoma mansoni OX=6183 PE=4 SV... [more]
A0A5J4NLT47.475e-10070.00Fumarate hydratase, class I OS=Paragonimus westerm... [more]
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BLAST of Fumarate hydratase, class I vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fumarate hydratase, class I vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000009591.17.323e-12286.92SMESG000009591.1[more]
SMESG000009591.12.067e-16886.92SMESG000009591.1[more]
SMESG000075499.10.000e+0100.00SMESG000075499.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026457 ID=SMED30026457|Name=Fumarate hydratase, class I|organism=Schmidtea mediterranea sexual|type=transcript|length=1830bp
ATTGGTTATTAAGTAATAAATTCAAATATTTAATTTCAGTTTTATTTTAC
GTGAATTATTTTATATTTTCGATTTTAATGAATTTATTAAATAAAATTAT
TCGAGCACCTGCAAAATATGTTCGGTTTTACTGTGCAAATGCTAAACCTT
TTTATTTTCAAGATATATTTGAGGTAGAAAAGTGTCCATATCATGACGTT
GAAATGGAAAAGCTAACCGGTGACTATGTGAAAATTGAAAAATTTATGAA
TAAAGAAATTTTGAAAGTGGACAATAAAGCTTTAACAATTTTGGCGGAAC
GAGCAATGATTGATATTGCTCATTTATTGAGACCTGCTCATTTGAAATCA
TTATCTGGAATTTTATCCGATCCAGAATCATCGAAAAATGATAAATTTGT
GGCATTGGAATTGTTGAAAAATGCCAGTATTGCAGCAAATATGGTTCTTC
CCGGTTGTCAAGATACCGGAACTGCGATAATAATGGGAAAGAAAGGCCAA
ATGGTCTGGACCGATGGAAAGGATGCGGAAGCATTGTCTCGGGGTGTGTA
CAACACTTACACCAGCAAAAATTTGAGATATTCACAAGTAGCTCCGGTGA
CGATGTACAATGAGCAGAATACCGGCACGAATTTGCCTGCTCAAATTGAG
ATTTACGCCACAGATGGCGACGAATACAAATTCCTATTTATTGCCAAAGG
CGGCGGGTCGGCTAATAAAACGTTTCTCTATCAACAGACAAAAGCTTTGC
TAAATCCAGAGAAATTGTTGGCTTTTGTCAATGAAAAAAATTAAAACATT
GGGCACTGCGGCGTGTCCTCCTTATCATTTGGCAGTGGTTATCGGGGGGA
CTAGTGCAGAAACCACATTGAAAACAGTGAAATTGGCCTCGACTAAATAT
CTCGACAACCTCTCAACCTCTGGTAATGCTCACGGTCGCGCTTTCCGTGA
CCTGGAACTCGAGGCCCAGATCCTTGCACTGACTCGGTCGGTTGGAATCG
GAGCGCAATTCGGGGGCAAATATTTCTGTCACGATGTGCGCGTCGTGCGA
CTACCTCGTCACGGTGCCTCTTGTCCCGTGGGTATCGGGGTCTCGTGTTC
CGCCGACAGGCAAATCCTCGGAAAAATTACCCGTAACGGATTGTTCCTGG
AGAAATTGGAACGCGATCCCAGCAAATACATGCCCGAGATCACCGATTCA
AATCTCTCTCAGGACGTGGTAAAAGTCGATCTCAATCGACCAATGAAAGA
AGTTTTGGGATTATTATCGAAATATCCAATAAAAACCCGGCTAAGTCTCT
CGGGGACTCTGATCGTTGCTAGGGACATTGCGCACGCGAAACTGCTGGAG
CGAGTCAACAAAGGCCAATCGCTGCCCGATTACATCCTGAAACATCCGGT
TTATTATGCAGGACCCGCTAAAACTCCCCCCGGAATGCCCAGCGGTTCCT
TTGGTCCGACAACCGCAGGCCGCATGGACTCGTATGTTGCGCAGTTCCAG
AAATGTGGCGGCAGCATGATTATGCTTGCCAAGGGCAACCGCAGCAAGCA
GGTGACTGACGCCTGCAAAAAGTACGGCGGGTTCTATCTGGGGTCGATTG
GAGGCCCTGCGGCCGTCCTCGCCCAGAATTGCATCAAGAAAGTCGAGGTG
GTCGAGTACGAGGAACTCGGAATGGAGGCAATTTGGCGGGTCGAGGTGGA
GAACTTCCCGGCCTTTATTGTGGTCGATGACAAAGGTAATGATTTCTTTC
AGGAATGGCAAGTCGAATAGGAATCTGTGATAAGCAATCGGATTTACAAT
ATTTGTGATTAAATATATCGTCTTGACTTT
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protein sequence of SMED30026457-orf-1

>SMED30026457-orf-1 ID=SMED30026457-orf-1|Name=SMED30026457-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=264bp
WLLSNKFKYLISVLFYVNYFIFSILMNLLNKIIRAPAKYVRFYCANAKPF
YFQDIFEVEKCPYHDVEMEKLTGDYVKIEKFMNKEILKVDNKALTILAER
AMIDIAHLLRPAHLKSLSGILSDPESSKNDKFVALELLKNASIAANMVLP
GCQDTGTAIIMGKKGQMVWTDGKDAEALSRGVYNTYTSKNLRYSQVAPVT
MYNEQNTGTNLPAQIEIYATDGDEYKFLFIAKGGGSANKTFLYQQTKALL
NPEKLLAFVNEKN*
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protein sequence of SMED30026457-orf-2

>SMED30026457-orf-2 ID=SMED30026457-orf-2|Name=SMED30026457-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=330bp
MKKIKTLGTAACPPYHLAVVIGGTSAETTLKTVKLASTKYLDNLSTSGNA
HGRAFRDLELEAQILALTRSVGIGAQFGGKYFCHDVRVVRLPRHGASCPV
GIGVSCSADRQILGKITRNGLFLEKLERDPSKYMPEITDSNLSQDVVKVD
LNRPMKEVLGLLSKYPIKTRLSLSGTLIVARDIAHAKLLERVNKGQSLPD
YILKHPVYYAGPAKTPPGMPSGSFGPTTAGRMDSYVAQFQKCGGSMIMLA
KGNRSKQVTDACKKYGGFYLGSIGGPAAVLAQNCIKKVEVVEYEELGMEA
IWRVEVENFPAFIVVDDKGNDFFQEWQVE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004333fumarate hydratase activity
GO:0016829lyase activity
GO:0016836hydro-lyase activity
GO:0003824catalytic activity
Vocabulary: biological process
TermDefinition
GO:0006091generation of precursor metabolites and energy
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR004646Fe-S_hydro-lyase_TtdA-typ_cat
IPR004647Fe-S_hydro-lyase_TtdB-typ_cat
IPR020557Fumarate_lyase_CS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004646Fe-S hydro-lyase, tartrate dehydratase alpha-type, catalytic domainPFAMPF05681Fumerasecoord: 98..263
e-value: 8.0E-48
score: 163.2
NoneNo IPR availablePANTHERPTHR30389FUMARATE HYDRATASE-RELATEDcoord: 48..263
NoneNo IPR availablePANTHERPTHR30389:SF0FUMARATE HYDRATASE CLASS I, ANAEROBICcoord: 48..263
NoneNo IPR availableTMHMMTMhelixcoord: 10..29