Kunitz-type serine protease inhibitor BmKTT-2

Overview
NameKunitz-type serine protease inhibitor BmKTT-2
Smed IDSMED30026393
Length (bp)419
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz-type serine protease inhibitor BmKTT-2 (SMED30026393) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz-type serine protease inhibitor BmKTT-2 (SMED30026393) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30026393

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30026393 SmedASXL_018636SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30026393 SmedASXL_018636SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
gutSMED30026393 dd_Smed_v4_71_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30026393 dd_Smed_v6_71_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30026393 dd_Smed_v4_71_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30026393 dd_Smed_v6_71_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 69.707 bits (169), Expect = 2.623e-14
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ P   G C AY PR+ +N Q+G C+SF+YGGC+ NGNNF + E CE  C
Sbjct:  293 CSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 343          

HSP 2 Score: 46.9802 bits (110), Expect = 2.271e-6
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 3
Query:  159 APECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            A EC +P  +  C     R++F+ Q+ NC +F +G C  N N+F T E C   C+
Sbjct:  232 AAECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGHCHRNLNHFETYEACMLACM 286          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 69.707 bits (169), Expect = 3.632e-14
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ P   G C AY PR+ +N Q+G C+SF+YGGC+ NGNNF + E CE  C
Sbjct:  386 CSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 436          

HSP 2 Score: 46.595 bits (109), Expect = 3.032e-6
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 3
Query:  159 APECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            A EC +P  +  C     R++F+ Q+ NC +F +G C  N N+F T E C   C+
Sbjct:  325 AAECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGHCHRNLNHFETYEACMLACM 379          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 61.2326 bits (147), Expect = 3.235e-11
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   TG C A  PR+YF++  G C  FIYGGC  N NNF + + C A C
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 360          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 61.2326 bits (147), Expect = 3.240e-11
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   TG C A  PR+YF++  G C  FIYGGC  N NNF + + C A C
Sbjct:  320 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 370          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 61.2326 bits (147), Expect = 3.241e-11
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   TG C A  PR+YF++  G C  FIYGGC  N NNF + + C A C
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 360          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 65.0846 bits (157), Expect = 6.346e-13
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +RA  C+ PK+ G C  Y  RFYFN     C+ F +GGC+ N NNF  +E+CE+ C
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589          

HSP 2 Score: 61.2326 bits (147), Expect = 1.151e-11
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  P   G+C  Y PR++FN Q+G CE F YG C  N NNF     CE +C+
Sbjct: 2177 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2228          

HSP 3 Score: 60.077 bits (144), Expect = 3.560e-11
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P+ +G C   F R++F+ +  NC+ F Y GCQ NGNNF + EEC A C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787          

HSP 4 Score: 58.151 bits (139), Expect = 1.285e-10
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            + ++ +S    C    + G CA  F RF F+     C SF YGGC  N NNF T++EC  +C+
Sbjct:  855 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 917          

HSP 5 Score: 57.7658 bits (138), Expect = 2.184e-10
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C +P + G C  +  R+YFN+  G C  F YGGC  N N+F T +ECE  C
Sbjct: 1082 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133          

HSP 6 Score: 55.4546 bits (132), Expect = 1.338e-9
Identity = 26/64 (40.62%), Postives = 33/64 (51.56%), Query Frame = 3
Query:  138 FSLGESRA---PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S+  SR     EC      G C   F R+++N  S  CE F Y GC  NGNN+ + E CE RC
Sbjct:  410 YSIQSSRTVNTKECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473          

HSP 7 Score: 51.6026 bits (122), Expect = 3.089e-8
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISKI 356
            P C +P   G C     R+Y++  + +C  F Y GC  N NNF ++E+C+  C+  +Q+ +  K+
Sbjct: 2740 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCVLNIQSIKNGKV 2804          

HSP 8 Score: 45.4394 bits (106), Expect = 3.553e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+  K +G    Y  ++YFN+++  CE F++ G   N N F T+ ECE  C
Sbjct: 2241 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2291          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 64.6994 bits (156), Expect = 7.057e-13
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +RA  C+ PK+ G C  Y  RFYFN     C+ F +GGC+ N NNF  +E+CE+ C
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589          

HSP 2 Score: 61.2326 bits (147), Expect = 1.267e-11
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  P   G+C  Y PR++FN Q+G CE F YG C  N NNF     CE +C+
Sbjct: 2168 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2219          

HSP 3 Score: 59.6918 bits (143), Expect = 3.920e-11
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P+ +G C   F R++F+ +  NC+ F Y GCQ NGNNF + EEC A C
Sbjct:  728 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 778          

HSP 4 Score: 58.151 bits (139), Expect = 1.388e-10
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            + ++ +S    C    + G CA  F RF F+     C SF YGGC  N NNF T++EC  +C+
Sbjct:  846 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 908          

HSP 5 Score: 57.3806 bits (137), Expect = 2.405e-10
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C +P + G C  +  R+YFN+  G C  F YGGC  N N+F T +ECE  C
Sbjct: 1073 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1124          

HSP 6 Score: 55.0694 bits (131), Expect = 1.531e-9
Identity = 26/64 (40.62%), Postives = 33/64 (51.56%), Query Frame = 3
Query:  138 FSLGESRA---PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S+  SR     EC      G C   F R+++N  S  CE F Y GC  NGNN+ + E CE RC
Sbjct:  410 YSIQSSRTVNTKECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473          

HSP 7 Score: 51.2174 bits (121), Expect = 3.304e-8
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISKI 356
            P C +P   G C     R+Y++  + +C  F Y GC  N NNF ++E+C+  C+  +Q+ +  K+
Sbjct: 2731 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCVLNIQSIKNGKV 2795          

HSP 8 Score: 45.4394 bits (106), Expect = 3.836e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+  K +G    Y  ++YFN+++  CE F++ G   N N F T+ ECE  C
Sbjct: 2232 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2282          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 64.6994 bits (156), Expect = 7.328e-13
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +RA  C+ PK+ G C  Y  RFYFN     C+ F +GGC+ N NNF  +E+CE+ C
Sbjct:  405 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 460          

HSP 2 Score: 61.2326 bits (147), Expect = 1.316e-11
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  P   G+C  Y PR++FN Q+G CE F YG C  N NNF     CE +C+
Sbjct: 2048 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2099          

HSP 3 Score: 59.6918 bits (143), Expect = 4.071e-11
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P+ +G C   F R++F+ +  NC+ F Y GCQ NGNNF + EEC A C
Sbjct:  608 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 658          

HSP 4 Score: 58.151 bits (139), Expect = 1.483e-10
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            + ++ +S    C    + G CA  F RF F+     C SF YGGC  N NNF T++EC  +C+
Sbjct:  726 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 788          

HSP 5 Score: 57.3806 bits (137), Expect = 2.521e-10
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C +P + G C  +  R+YFN+  G C  F YGGC  N N+F T +ECE  C
Sbjct:  953 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1004          

HSP 6 Score: 55.0694 bits (131), Expect = 1.544e-9
Identity = 26/64 (40.62%), Postives = 33/64 (51.56%), Query Frame = 3
Query:  138 FSLGESRA---PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S+  SR     EC      G C   F R+++N  S  CE F Y GC  NGNN+ + E CE RC
Sbjct:  281 YSIQSSRTVNTKECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 344          

HSP 7 Score: 51.2174 bits (121), Expect = 3.397e-8
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISKI 356
            P C +P   G C     R+Y++  + +C  F Y GC  N NNF ++E+C+  C+  +Q+ +  K+
Sbjct: 2611 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCVLNIQSIKNGKV 2675          

HSP 8 Score: 45.4394 bits (106), Expect = 3.906e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+  K +G    Y  ++YFN+++  CE F++ G   N N F T+ ECE  C
Sbjct: 2112 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2162          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 64.3142 bits (155), Expect = 1.073e-12
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +RA  C+ PK+ G C  Y  RFYFN     C+ F +GGC+ N NNF  +E+CE+ C
Sbjct:  313 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368          

HSP 2 Score: 60.8474 bits (146), Expect = 1.853e-11
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  P   G+C  Y PR++FN Q+G CE F YG C  N NNF     CE +C+
Sbjct: 1575 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 1626          

HSP 3 Score: 59.3066 bits (142), Expect = 5.569e-11
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P+ +G C   F R++F+ +  NC+ F Y GCQ NGNNF + EEC A C
Sbjct:  516 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 566          

HSP 4 Score: 57.7658 bits (138), Expect = 2.068e-10
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            + ++ +S    C    + G CA  F RF F+     C SF YGGC  N NNF T++EC  +C+
Sbjct:  634 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 696          

HSP 5 Score: 56.9954 bits (136), Expect = 3.515e-10
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C +P + G C  +  R+YFN+  G C  F YGGC  N N+F T +ECE  C
Sbjct:  861 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 912          

HSP 6 Score: 54.6842 bits (130), Expect = 2.216e-9
Identity = 26/64 (40.62%), Postives = 33/64 (51.56%), Query Frame = 3
Query:  138 FSLGESRA---PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S+  SR     EC      G C   F R+++N  S  CE F Y GC  NGNN+ + E CE RC
Sbjct:  189 YSIQSSRTVNTKECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252          

HSP 7 Score: 50.8322 bits (120), Expect = 4.734e-8
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISKI 356
            P C +P   G C     R+Y++  + +C  F Y GC  N NNF ++E+C+  C+  +Q+ +  K+
Sbjct: 2138 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCVLNIQSIKNGKV 2202          

HSP 8 Score: 45.0542 bits (105), Expect = 5.134e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+  K +G    Y  ++YFN+++  CE F++ G   N N F T+ ECE  C
Sbjct: 1639 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 1689          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 63.929 bits (154), Expect = 1.242e-12
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +RA  C+ PK+ G C  Y  RFYFN     C+ F +GGC+ N NNF  +E+CE+ C
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589          

HSP 2 Score: 60.4622 bits (145), Expect = 2.023e-11
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  P   G+C  Y PR++FN Q+G CE F YG C  N NNF     CE +C+
Sbjct: 1651 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 1702          

HSP 3 Score: 59.3066 bits (142), Expect = 6.198e-11
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P+ +G C   F R++F+ +  NC+ F Y GCQ NGNNF + EEC A C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787          

HSP 4 Score: 57.7658 bits (138), Expect = 2.194e-10
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            + ++ +S    C    + G CA  F RF F+     C SF YGGC  N NNF T++EC  +C+
Sbjct:  855 VMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 917          

HSP 5 Score: 56.9954 bits (136), Expect = 3.875e-10
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C +P + G C  +  R+YFN+  G C  F YGGC  N N+F T +ECE  C
Sbjct: 1082 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133          

HSP 6 Score: 54.6842 bits (130), Expect = 2.419e-9
Identity = 26/64 (40.62%), Postives = 33/64 (51.56%), Query Frame = 3
Query:  138 FSLGESRA---PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S+  SR     EC      G C   F R+++N  S  CE F Y GC  NGNN+ + E CE RC
Sbjct:  410 YSIQSSRTVNTKECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473          

HSP 7 Score: 50.8322 bits (120), Expect = 5.167e-8
Identity = 21/65 (32.31%), Postives = 36/65 (55.38%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISKI 356
            P C +P   G C     R+Y++  + +C  F Y GC  N NNF ++E+C+  C+  +Q+ +  K+
Sbjct: 2214 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCVLNIQSIKNGKV 2278          

HSP 8 Score: 45.0542 bits (105), Expect = 5.547e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+  K +G    Y  ++YFN+++  CE F++ G   N N F T+ ECE  C
Sbjct: 1715 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 1765          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0077545)

HSP 1 Score: 66.6254 bits (161), Expect = 8.937e-15
Identity = 26/53 (49.06%), Postives = 38/53 (71.70%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            +C++PK+TG C A F R+ +N+ + +CE F+YGGC  N NNF + E+CE  CL
Sbjct:   54 DCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQACL 106          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0346581)

HSP 1 Score: 66.6254 bits (161), Expect = 8.937e-15
Identity = 26/53 (49.06%), Postives = 38/53 (71.70%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            +C++PK+TG C A F R+ +N+ + +CE F+YGGC  N NNF + E+CE  CL
Sbjct:   54 DCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQACL 106          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0077556)

HSP 1 Score: 60.8474 bits (146), Expect = 6.869e-13
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQ 335
            C +PK  G+C  +  R+ +N ++GNCESFIY GC +  NNF T EEC   C++ L+
Sbjct:   41 CRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDCMQRLR 96          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0446100)

HSP 1 Score: 60.8474 bits (146), Expect = 6.869e-13
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQ 335
            C +PK  G+C  +  R+ +N ++GNCESFIY GC +  NNF T EEC   C++ L+
Sbjct:   41 CRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDCMQRLR 96          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301839)

HSP 1 Score: 59.6918 bits (143), Expect = 6.019e-11
Identity = 25/52 (48.08%), Postives = 31/52 (59.62%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            CNEP  TG C  +  ++YFN  S  CE F YGGC   GN F+ + EC+  CL
Sbjct: 2196 CNEPVTTGPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVCL 2247          

HSP 2 Score: 54.6842 bits (130), Expect = 3.138e-9
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C++P   G C  +   + +N   G C+SF YGGC  N N F T EEC ARC
Sbjct: 1943 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 1994          

HSP 3 Score: 53.5286 bits (127), Expect = 6.400e-9
Identity = 21/53 (39.62%), Postives = 31/53 (58.49%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            P C +P ++G CA  F R+Y++ ++  C  F YGGC+ N NN+ T   C   C
Sbjct: 1728 PTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNC 1780          

HSP 4 Score: 53.1434 bits (126), Expect = 1.087e-8
Identity = 22/51 (43.14%), Postives = 28/51 (54.90%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  PK+TG C  Y  ++YF+   G C  F YGGC  N N F +  EC+  C
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTC 1662          

HSP 5 Score: 52.7582 bits (125), Expect = 1.479e-8
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  PK  G C  +  ++YF++    CE F YGGC    N F+++E+C+  C
Sbjct: 1671 CLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC 1721          

HSP 6 Score: 52.373 bits (124), Expect = 1.759e-8
Identity = 23/51 (45.10%), Postives = 27/51 (52.94%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P + G CA Y   +Y++ Q   C  F YGGC  N N F T E C ARC
Sbjct: 1792 CEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARC 1842          

HSP 7 Score: 51.9878 bits (123), Expect = 2.281e-8
Identity = 22/57 (38.60%), Postives = 32/57 (56.14%), Query Frame = 3
Query:  153 SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            SR   C+E    G C+ +  +++F+ + G C  F YG C  NGN F T  +C+ RCL
Sbjct: 1860 SRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCL 1916          

HSP 8 Score: 50.447 bits (119), Expect = 9.940e-8
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 3
Query:  168 CNEPKKTGMC---AAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P  TG C   + +  R+Y++ ++GNC SFIY GC  N NNF + E C  +C
Sbjct: 2261 CLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQC 2314          

HSP 9 Score: 48.9062 bits (115), Expect = 2.945e-7
Identity = 25/72 (34.72%), Postives = 38/72 (52.78%), Query Frame = 3
Query:  120 LIFCVIFSLGESRAPE---CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            L FC+   + E  AP    C EP + G C      ++++ ++  C +F Y GC  NGN F T ++CE +C E
Sbjct: 2118 LNFCIGEPVVEPSAPTYSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGNGNRFETRDQCERQCGE 2189          

HSP 10 Score: 45.4394 bits (106), Expect = 5.193e-6
Identity = 20/57 (35.09%), Postives = 29/57 (50.88%), Query Frame = 3
Query:  150 ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            E    +C    + G C     R+++N   G C+ F+Y GC  N NN+ T EEC+  C
Sbjct: 2008 EHDTSKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNEC 2064          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Match: tfpia (tissue factor pathway inhibitor a [Source:ZFIN;Acc:ZDB-GENE-030711-1])

HSP 1 Score: 63.5438 bits (153), Expect = 1.633e-12
Identity = 28/52 (53.85%), Postives = 33/52 (63.46%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C   K  G C A   RFYF+I +G CE F YGGCQ N NNF T+++CE  CL
Sbjct:   42 CALKKDEGPCKAMKDRFYFDIDTGRCEPFEYGGCQGNANNFETLQDCEEMCL 93          

HSP 2 Score: 54.6842 bits (130), Expect = 2.472e-9
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 3
Query:  150 ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            E+++P C+   + G C    PR++F+ +S  C+ F YGGC  N NNF TI+ C+ RC
Sbjct:   96 ENKSP-CHLEDEPGPCRGLVPRYFFDQKSQECKQFFYGGCFGNANNFKTIKACQQRC 151          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Match: tfpia (tissue factor pathway inhibitor a [Source:ZFIN;Acc:ZDB-GENE-030711-1])

HSP 1 Score: 63.5438 bits (153), Expect = 1.829e-12
Identity = 28/52 (53.85%), Postives = 33/52 (63.46%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C   K  G C A   RFYF+I +G CE F YGGCQ N NNF T+++CE  CL
Sbjct:   41 CALKKDEGPCKAMKDRFYFDIDTGRCEPFEYGGCQGNANNFETLQDCEEMCL 92          

HSP 2 Score: 54.6842 bits (130), Expect = 2.637e-9
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 3
Query:  150 ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            E+++P C+   + G C    PR++F+ +S  C+ F YGGC  N NNF TI+ C+ RC
Sbjct:   95 ENKSP-CHLEDEPGPCRGLVPRYFFDQKSQECKQFFYGGCFGNANNFKTIKACQQRC 150          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:ZFIN;Acc:ZDB-GENE-061013-323])

HSP 1 Score: 61.6178 bits (148), Expect = 1.323e-11
Identity = 28/53 (52.83%), Postives = 33/53 (62.26%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
             C  P   G C A FPRF+++I    C+SFIYGGC  N NNF +  ECEA CL
Sbjct:  132 HCRFPSAVGNCRAAFPRFFYDITDQTCKSFIYGGCGGNENNFYSQAECEASCL 184          

HSP 2 Score: 48.521 bits (114), Expect = 5.051e-7
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C  P  TG C A   RFY+N  +G C+ FIYGGC  N NN+ + + C   C
Sbjct:  227 KCQSPAVTGNCRASIKRFYYN--NGVCQQFIYGGCGGNKNNYESEDSCMTAC 276          

HSP 3 Score: 48.1358 bits (113), Expect = 5.403e-7
Identity = 33/100 (33.00%), Postives = 47/100 (47.00%), Query Frame = 3
Query:   21 NNYKIFDYKLTIKYNSIQIFIIKPKMNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            NNY+  D  +T    +  + II  +    VI       I     S   +C  P  +G C A F  F+F   S +C+ FIYGGC+ N N + + EEC + C
Sbjct:  264 NNYESEDSCMT----ACTVKIIPKEQEGPVIP-----AIPKSAHSFEEKCVVPSDSGPCRASFRMFFFEASSQSCQPFIYGGCRGNLNRYGSEEECMSVC 354          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:ZFIN;Acc:ZDB-GENE-061013-323])

HSP 1 Score: 61.6178 bits (148), Expect = 1.361e-11
Identity = 28/53 (52.83%), Postives = 33/53 (62.26%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
             C  P   G C A FPRF+++I    C+SFIYGGC  N NNF +  ECEA CL
Sbjct:  132 HCRFPSAVGNCRAAFPRFFYDITDQTCKSFIYGGCGGNENNFYSQAECEASCL 184          

HSP 2 Score: 48.521 bits (114), Expect = 5.149e-7
Identity = 23/52 (44.23%), Postives = 30/52 (57.69%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +C  P  TG C A   RFY+N  +G C+ FIYGGC  N NN+ + + C   C
Sbjct:  227 KCQSPAVTGNCRASIKRFYYN--NGVCQQFIYGGCGGNKNNYESEDSCMTAC 276          

HSP 3 Score: 48.1358 bits (113), Expect = 5.455e-7
Identity = 33/100 (33.00%), Postives = 47/100 (47.00%), Query Frame = 3
Query:   21 NNYKIFDYKLTIKYNSIQIFIIKPKMNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            NNY+  D  +T    +  + II  +    VI       I     S   +C  P  +G C A F  F+F   S +C+ FIYGGC+ N N + + EEC + C
Sbjct:  264 NNYESEDSCMT----ACTVKIIPKEQEGPVIP-----AIPKSAHSFEEKCVVPSDSGPCRASFRMFFFEASSQSCQPFIYGGCRGNLNRYGSEEECMSVC 354          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Match: appb (amyloid beta (A4) precursor protein b [Source:ZFIN;Acc:ZDB-GENE-020220-1])

HSP 1 Score: 60.077 bits (144), Expect = 5.085e-11
Identity = 24/51 (47.06%), Postives = 30/51 (58.82%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C  P ++G C A  PR+YF  + G C SF +GGC  N NNF + E C A C
Sbjct:  306 CWAPARSGPCHAKLPRWYFVAEKGRCASFTFGGCGGNRNNFESEEYCMAVC 356          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:100486381])

HSP 1 Score: 63.5438 bits (153), Expect = 4.197e-12
Identity = 29/51 (56.86%), Postives = 32/51 (62.75%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            CN P   GMC AY PR+ +N Q   C+SFIYGGC  N NNF T E CE  C
Sbjct:  370 CNFPALQGMCKAYKPRWAYNKQLKQCQSFIYGGCGGNDNNFETKETCEDMC 420          

HSP 2 Score: 46.2098 bits (108), Expect = 4.380e-6
Identity = 21/53 (39.62%), Postives = 30/53 (56.60%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            EC +P  +  C     R+YF+ +  NC  FIYG C +N N+F+T E C   C+
Sbjct:  311 ECLKPPDSDDCGEEQTRWYFDAKKNNCFIFIYGNCNSNYNHFDTYESCMLTCM 363          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:NCBI gene;Acc:548483])

HSP 1 Score: 59.3066 bits (142), Expect = 4.908e-11
Identity = 29/65 (44.62%), Postives = 38/65 (58.46%), Query Frame = 3
Query:  129 CVIFSLGESRAPE-CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ F      AP  C  PK  G C+A   R+YFNI+S  CE F+Y GC  N NNF  +E+C++ C
Sbjct:  135 CMDFCSPRRDAPSFCYSPKDEGSCSASVTRYYFNIESKACEEFVYTGCGGNSNNFVKMEDCDSVC 199          

HSP 2 Score: 52.373 bits (124), Expect = 1.645e-8
Identity = 23/77 (29.87%), Postives = 40/77 (51.95%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            + +++  +L+  V  S  +     C  P   G C A  P +Y++  +  C+ F+YGGC  N N F ++E+CE  C +
Sbjct:    5 LQALMAASLLIQVFGSPMQGNTTVCLLPPDEGPCKALIPHYYYDRYTQTCQEFLYGGCDGNSNKFLSMEDCEKFCWK 81          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:NCBI gene;Acc:733874])

HSP 1 Score: 57.7658 bits (138), Expect = 3.856e-10
Identity = 25/54 (46.30%), Postives = 32/54 (59.26%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            P C      G C A FPR+YF+ +S NC SF YGGC    NN  +++EC  RC+
Sbjct:  194 PACTGKGVIGNCRASFPRWYFDAESQNCISFTYGGCGGTENNHKSVQECADRCI 247          

HSP 2 Score: 53.1434 bits (126), Expect = 1.413e-8
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISK 353
            C  P  TG C A F R+Y+++ +  C +F YGGC+ N NN+ ++E+C   C   L     S 
Sbjct:  268 CAAPSLTGPCRASFRRWYYDVTTATCVAFTYGGCRGNKNNYLSVEDCVKNCAGRLDDDHTSD 329          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Match: ENSXETT00000011536.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV462126.1:486:923:1 gene:ENSXETG00000005815.1 transcript:ENSXETT00000011536.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 4.699e-10
Identity = 23/47 (48.94%), Postives = 29/47 (61.70%), Query Frame = 3
Query:  183 KTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            K+G C A  P++Y+N + G C SF+Y GCQ NGN F   E C   CL
Sbjct:    9 KSGPCLALIPQWYYNKERGACHSFLYSGCQGNGNRFENKENCTTLCL 55          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 57.3806 bits (137), Expect = 4.908e-10
Identity = 23/51 (45.10%), Postives = 30/51 (58.82%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   TG C A  PR+YF++    C  FIYGGC  N NNF + + C A C
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 347          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 68.5514 bits (166), Expect = 4.760e-14
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ P   G C AY PR+ +N Q+G C+SF+YGGC+ NGNNF + E CE  C
Sbjct:  381 CSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESC 431          

HSP 2 Score: 46.595 bits (109), Expect = 2.424e-6
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 3
Query:  159 APECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            A EC +P  +  C     R++F+ Q+ NC +F +G C  N N+F T E C   C+
Sbjct:  320 ATECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGHCHHNLNHFETYEACMLACM 374          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Match: Spint5 (serine protease inhibitor, Kunitz type 5 [Source:MGI Symbol;Acc:MGI:3651687])

HSP 1 Score: 60.077 bits (144), Expect = 2.390e-12
Identity = 28/53 (52.83%), Postives = 34/53 (64.15%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            CN+P K G C+  F R+YFN +S  CESFI+ GC  N NNF T   CE RC+ 
Sbjct:   49 CNQPVKKGFCSFTFYRYYFNPESALCESFIFTGCGGNRNNFKTKYLCEVRCIH 101          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Match: Spint5 (serine protease inhibitor, Kunitz type 5 [Source:MGI Symbol;Acc:MGI:3651687])

HSP 1 Score: 60.077 bits (144), Expect = 2.776e-12
Identity = 28/53 (52.83%), Postives = 34/53 (64.15%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            CN+P K G C+  F R+YFN +S  CESFI+ GC  N NNF T   CE RC+ 
Sbjct:   49 CNQPVKKGFCSFTFYRYYFNPESALCESFIFTGCGGNRNNFKTKYLCEVRCIH 101          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Match: Spint2 (serine protease inhibitor, Kunitz type 2 [Source:MGI Symbol;Acc:MGI:1338031])

HSP 1 Score: 62.7734 bits (151), Expect = 2.842e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C   K  G C A  PR+++NI  G+C+ F+YGGC+ NGNN+ + EEC  +C
Sbjct:   38 CGVSKVVGKCRASIPRWWYNITDGSCQPFVYGGCEGNGNNYQSKEECLDKC 88          

HSP 2 Score: 51.2174 bits (121), Expect = 4.740e-8
Identity = 21/45 (46.67%), Postives = 30/45 (66.67%), Query Frame = 3
Query:  186 TGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            TG C A FPR+Y++ +  +C SFIYGGC+ N N++ + E C   C
Sbjct:  139 TGPCRAAFPRWYYDTEKNSCISFIYGGCRGNKNSYLSQEACMQHC 183          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Match: Spint3 (serine peptidase inhibitor, Kunitz type, 3 [Source:MGI Symbol;Acc:MGI:3651470])

HSP 1 Score: 58.9214 bits (141), Expect = 4.599e-12
Identity = 27/57 (47.37%), Postives = 36/57 (63.16%), Query Frame = 3
Query:  150 ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +S  P C  PK  G C A F R+Y+N ++G CE F YGGC+ N NNF +  +C+A C
Sbjct:   29 KSLPPMCILPKDIGGCRAVFVRWYYNSKTGKCEWFRYGGCKGNENNFPSRSQCQAVC 85          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Match: sp|P0DMJ5|VKT_HETCR (PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.663e-18
Identity = 37/76 (48.68%), Postives = 45/76 (59.21%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPE-CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M    +  LI    FS   ++A   C EPK  G C AYFPRFYF+ ++G C  FIYGGC+ NGNNF T+  C A C
Sbjct:    1 MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAIC 76          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Match: sp|P83606|BMTI6_RHIMP (Kunitz-type serine protease inhibitor 6 (Fragment) OS=Rhipicephalus microplus OX=6941 PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 1.030e-17
Identity = 36/58 (62.07%), Postives = 43/58 (74.14%), Query Frame = 3
Query:  162 PECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQ 335
            P CNEPK   +C  YFPR+Y+N +S  C+ FIYGGCQ+NGNNF T+EECE  CL  LQ
Sbjct:  233 PVCNEPKYPELCKGYFPRYYYNSRSKTCKKFIYGGCQSNGNNFLTLEECENTCLVDLQ 290          

HSP 2 Score: 70.8626 bits (172), Expect = 2.773e-14
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C     +G C  YFPR++FN+++G CE FIYGGCQ N NN  T +ECE RCL
Sbjct:    7 CKPTYDSGPCKGYFPRWWFNVKTGQCEEFIYGGCQGNKNNHVTRKECETRCL 58          

HSP 3 Score: 65.855 bits (159), Expect = 1.987e-12
Identity = 27/52 (51.92%), Postives = 33/52 (63.46%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C      G C AY PR++FN+++G CE FIYGGCQ N NN+ T   CE  CL
Sbjct:  139 CKPTHDRGPCKAYIPRWWFNVKTGQCEQFIYGGCQGNKNNYETKSICETNCL 190          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Match: sp|B2G331|VKT1_HETCR (TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.177e-16
Identity = 36/76 (47.37%), Postives = 44/76 (57.89%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPE-CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M    +  LI    FS   ++A   C EPK  G C AYF RFYF+ ++G C  FIYGGC+ NGNNF T+  C A C
Sbjct:    1 MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFRRFYFDSETGKCTVFIYGGCEGNGNNFETLRACRAIC 76          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Match: sp|C0HK74|VKT3_HETMG (Kunitz-type serine protease inhibitor HMGS2 OS=Heteractis magnifica OX=38281 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.352e-16
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C EPK  G C AYFPRFYF+ ++G C  FIYGGC+ NGNNF T+  C A C
Sbjct:    4 CLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAIC 54          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Match: sp|P0DN09|VKT5_ANEVI (U-actitoxin-Avd3h OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.828e-16
Identity = 33/71 (46.48%), Postives = 44/71 (61.97%), Query Frame = 3
Query:  108 VIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            ++F L F ++  +      +C  PK  G C A FPR+Y+N  S  CE FIYGGC+ N NNF+T+EECE  C
Sbjct:    1 MVFLLCFFLVADVSYGINGDCELPKVVGPCRARFPRYYYNSSSKRCEKFIYGGCRGNANNFHTLEECEKVC 71          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Match: C1IBY4 (Tabserpin OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.337e-19
Identity = 37/75 (49.33%), Postives = 50/75 (66.67%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M  + +  ++F V+     ++ P C++PK  G C A FP+FYFN  SG CE+FIYGGC  N NNFNT+EEC A+C
Sbjct:    1 MKVLSLIFVLFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYFNSSSGQCEAFIYGGCGGNENNFNTLEECNAKC 75          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Match: C8YJ95 (Serine protease inhibitor 3 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.643e-19
Identity = 37/75 (49.33%), Postives = 50/75 (66.67%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M  + +  +IF V+     ++ P C++PK  G C A FP+FYFN  SG CE+FIYGGC  N NNF+T+EEC A+C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYFNSSSGQCEAFIYGGCGGNENNFSTLEECNAKC 75          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Match: C8YJ94 (Serine protease inhibitor 2 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 8.532e-19
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M  + +  +IF V+     ++ P C++PK  G C A FP+FY+N  SG C++FIYGGC  N NNFNT+EEC A+C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYYNSSSGQCQAFIYGGCGGNENNFNTLEECNAKC 75          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Match: C8YJ97 (Serine protease inhibitor 5 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.547e-18
Identity = 37/75 (49.33%), Postives = 48/75 (64.00%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M  + +  +IF V+     ++ P C++PK    C A FP+FYFN  SG CE FIYGGC  N NNFNT+EEC A+C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVRRCFAAFPKFYFNSSSGQCEGFIYGGCGGNENNFNTLEECNAKC 75          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Match: C8YJ96 (Serine protease inhibitor 4 OS=Tabanus yao OX=485572 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.031e-16
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            M  + +  +IF V+     ++ P C++PK  G C A FP+FYFN  S   E+F YGGC  N NNFNT+EEC A+C
Sbjct:    1 MKVLSLIFVIFSVLVLFASAKDPVCDQPKAVGRCFAAFPKFYFNSSSAQGEAFFYGGCGGNENNFNTLEECNAKC 75          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Match: ENSAMXT00000015806.2 (tissue factor pathway inhibitor-like [Source:NCBI gene;Acc:103025270])

HSP 1 Score: 64.6994 bits (156), Expect = 6.005e-13
Identity = 28/48 (58.33%), Postives = 33/48 (68.75%), Query Frame = 3
Query:  180 KKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            K  G C A   RFYF+I SG CESF YGGCQ N NNF T+++CE  C+
Sbjct:   40 KDEGPCKAVKDRFYFDIDSGRCESFEYGGCQGNANNFQTLDQCEEMCV 87          

HSP 2 Score: 52.7582 bits (125), Expect = 9.849e-9
Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = 3
Query:  126 FCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
             CV+    E+++P C+   + G C    PR++F+ +   C  F YGGC  N NNF T++EC  RC
Sbjct:   85 MCVV---KENKSP-CHLEDEPGPCRGLVPRYFFDSRKQECRRFFYGGCFGNANNFKTLKECRDRC 145          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Match: paplnb (papilin-like [Source:NCBI gene;Acc:103035042])

HSP 1 Score: 64.3142 bits (155), Expect = 1.265e-12
Identity = 28/56 (50.00%), Postives = 33/56 (58.93%), Query Frame = 3
Query:  159 APECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            A  C+ P+  G C  Y  RFYF+  +  C  F YGGCQ NGNNF T+EEC   C E
Sbjct:  465 AEVCSLPRDVGSCYEYKSRFYFDSSARTCTQFWYGGCQGNGNNFATVEECRRMCGE 520          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Match: paplnb (papilin-like [Source:NCBI gene;Acc:103035042])

HSP 1 Score: 63.929 bits (154), Expect = 1.772e-12
Identity = 28/56 (50.00%), Postives = 33/56 (58.93%), Query Frame = 3
Query:  159 APECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            A  C+ P+  G C  Y  RFYF+  +  C  F YGGCQ NGNNF T+EEC   C E
Sbjct:  494 AEVCSLPRDVGSCYEYKSRFYFDSSARTCTQFWYGGCQGNGNNFATVEECRRMCGE 549          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Match: wfikkn2b (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 [Source:NCBI gene;Acc:103024700])

HSP 1 Score: 63.929 bits (154), Expect = 1.924e-12
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 3
Query:  129 CVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   GE   P C+ P   G C AY PR+ ++I  G C+ FIYGGC+ N NNF T E CE  C
Sbjct:  362 CLLSCQGELAVP-CSLPSLQGPCKAYEPRWAYSITLGQCQPFIYGGCEGNENNFETRETCEDSC 424          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Match: lrp11 (LDL receptor related protein 11 [Source:NCBI gene;Acc:103032685])

HSP 1 Score: 60.8474 bits (146), Expect = 2.207e-11
Identity = 22/53 (41.51%), Postives = 32/53 (60.38%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            C EP   G C   FPR+Y++  +G C+ F+YGGC+ N NNF    +C   C++
Sbjct:  417 CMEPPVVGPCRGTFPRWYYDAAAGECKHFLYGGCKGNRNNFLQQTDCVNECIQ 469          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 68.9366 bits (167), Expect = 1.887e-15
Identity = 32/62 (51.61%), Postives = 41/62 (66.13%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            +F+L + R   C  P K G C A FPR++FN  +  CESFIYGGC +N NNF T++EC A C
Sbjct:   17 VFTLEDQRV-TCTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAAC 77          

HSP 2 Score: 50.447 bits (119), Expect = 1.124e-8
Identity = 21/53 (39.62%), Postives = 30/53 (56.60%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            C    +TG C A+  R+++N+  G C  F+YGGC  N N F  +E C   C+E
Sbjct:   87 CRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESCVFSCIE 139          

HSP 3 Score: 48.9062 bits (115), Expect = 4.226e-8
Identity = 27/66 (40.91%), Postives = 34/66 (51.52%), Query Frame = 3
Query:  129 CVIFSLGESRAP-ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            CV   + +   P EC     TG C   F RFY+NIQ  +C  F Y GC  N NNF + + C + CL
Sbjct:  133 CVFSCIEKPNTPRECLSRHDTGNCQGNFRRFYYNIQKRSCLPFSYSGCGGNNNNFISAKTCLSTCL 198          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 4.608e-10
Identity = 23/44 (52.27%), Postives = 29/44 (65.91%), Query Frame = 3
Query:  189 GMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            G C AY PR ++N  +  CE F YGGC  NGNNF ++ EC A+C
Sbjct:   10 GPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53          

HSP 2 Score: 52.7582 bits (125), Expect = 1.495e-9
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 3
Query:  159 APE-CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISK 353
            AP+ C    + G+C A   R++FN+ SG+CE F YGGCQ N NNF     C   C+      +I K
Sbjct:   71 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKK 136          

HSP 3 Score: 51.6026 bits (122), Expect = 3.834e-9
Identity = 22/44 (50.00%), Postives = 29/44 (65.91%), Query Frame = 3
Query:  189 GMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            G C A  PRF++N ++ NCESF YGGC  N NNF++   C + C
Sbjct:  174 GECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 217          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 5.262e-10
Identity = 23/44 (52.27%), Postives = 29/44 (65.91%), Query Frame = 3
Query:  189 GMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            G C AY PR ++N  +  CE F YGGC  NGNNF ++ EC A+C
Sbjct:   10 GPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53          

HSP 2 Score: 52.7582 bits (125), Expect = 1.813e-9
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 3
Query:  159 APE-CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE*LQTFEISK 353
            AP+ C    + G+C A   R++FN+ SG+CE F YGGCQ N NNF     C   C+      +I K
Sbjct:   70 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKK 135          

HSP 3 Score: 51.6026 bits (122), Expect = 4.292e-9
Identity = 22/44 (50.00%), Postives = 29/44 (65.91%), Query Frame = 3
Query:  189 GMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            G C A  PRF++N ++ NCESF YGGC  N NNF++   C + C
Sbjct:  173 GECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 216          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 52.7582 bits (125), Expect = 2.658e-9
Identity = 21/60 (35.00%), Postives = 35/60 (58.33%), Query Frame = 3
Query:  141 SLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            ++ E  A EC+EP     C +   ++Y++     C++F+YGGC +N N F+T E C+  C
Sbjct:  332 AIAEFPASECHEPPDVTHCGSARTKWYYDSGLNACDTFVYGGCDSNRNRFDTYEGCKRWC 391          

HSP 2 Score: 43.1282 bits (100), Expect = 6.076e-6
Identity = 18/51 (35.29%), Postives = 26/51 (50.98%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ P   G C  +   + +N     C+  +YGGC+ N NNF + E CE  C
Sbjct:  399 CHLPPVQGPCRDWERHWAYNPLIKQCQLLVYGGCEGNENNFESREACEDNC 449          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008261.1 (pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene:ENSPMAG00000007479.1 transcript:ENSPMAT00000008261.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 5.316e-8
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C+ P   G C A+  R+ +N     C +F+YGGC+ NGN+F +   CE  C
Sbjct:   21 CSLPPVQGPCKAWETRWSYNPLGKRCATFLYGGCEGNGNSFASRSACERAC 71          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 72.0182 bits (175), Expect = 6.504e-18
Identity = 31/52 (59.62%), Postives = 36/52 (69.23%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  PKK G C A  PR +FN QSG CE FIYGGC  N NNF+T+EEC+  C+
Sbjct:   14 CTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKACM 65          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 64.6994 bits (156), Expect = 3.617e-15
Identity = 28/52 (53.85%), Postives = 36/52 (69.23%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            EC +PK TG C AYF R+++N  S  C+ F+YGGCQ N NNF +  ECE +C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 64.6994 bits (156), Expect = 3.617e-15
Identity = 28/52 (53.85%), Postives = 36/52 (69.23%), Query Frame = 3
Query:  165 ECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            EC +PK TG C AYF R+++N  S  C+ F+YGGCQ N NNF +  ECE +C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 63.1586 bits (152), Expect = 1.925e-14
Identity = 27/52 (51.92%), Postives = 35/52 (67.31%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C  PK  G C A   RFY+N + G C+SF++GGC  NGNNF T + CEA+C+
Sbjct:   13 CKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACEAKCM 64          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Match: EDO41922 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S322])

HSP 1 Score: 59.6918 bits (143), Expect = 3.827e-13
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 3
Query:  144 LGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            +G  +   C+ P  TG C AY P FY++++   C+ FIYGGC  N N F+T E+CE  C+
Sbjct:    1 MGGEQPSFCSLPAVTGRCMAYIPSFYYDMKEEKCKEFIYGGCNGNLNRFDTQEKCEEACM 60          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Match: ENSORLT00000012975.2 (pep primary_assembly:ASM223467v1:12:15740273:15760140:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000012975.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 5.266e-12
Identity = 26/52 (50.00%), Postives = 34/52 (65.38%), Query Frame = 3
Query:  168 CNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCL 323
            C +   +G+C  YF R+++N + G CE FIYGGC  N NNF T +EC  RCL
Sbjct:  125 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQRCL 176          

HSP 2 Score: 57.3806 bits (137), Expect = 1.015e-10
Identity = 26/61 (42.62%), Postives = 36/61 (59.02%), Query Frame = 3
Query:  135 IFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEAR 317
            + SL  +    C +   +G+C  YF R+++N + G CE FIYGGC  N NNF T +EC  R
Sbjct:   62 LVSLPSASTGVCAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQR 122          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Match: spint1a (kunitz-type protease inhibitor 1 [Source:NCBI gene;Acc:101175207])

HSP 1 Score: 62.003 bits (149), Expect = 7.367e-12
Identity = 33/82 (40.24%), Postives = 42/82 (51.22%), Query Frame = 3
Query:   93 KMNSIVIFTL------IFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            K N  VI T+      +  V+    E     C  PKK G C   FPR+Y+N  S  CE+F +GGC+ N NN+ T EEC   C
Sbjct:  203 KFNLTVIDTIGQSDSTVVTVLVLTPEQSMDHCLVPKKVGPCRGSFPRWYYNAASEKCETFFFGGCRQNKNNYLTEEECAMAC 284          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 59.3066 bits (142), Expect = 6.786e-11
Identity = 28/68 (41.18%), Postives = 40/68 (58.82%), Query Frame = 3
Query:  117 TLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            T++  ++ +  ES    C  PKK G C   FPR+++N  S  CE FI+GGC+ N NN+   EEC+  C
Sbjct:  223 TVLTVLVLTPQESEH-HCMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 59.3066 bits (142), Expect = 7.365e-11
Identity = 28/68 (41.18%), Postives = 40/68 (58.82%), Query Frame = 3
Query:  117 TLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            T++  ++ +  ES    C  PKK G C   FPR+++N  S  CE FI+GGC+ N NN+   EEC+  C
Sbjct:  223 TVLTVLVLTPQESEH-HCMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Match: wfikkn2a (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:101168544])

HSP 1 Score: 58.5362 bits (140), Expect = 1.245e-10
Identity = 28/64 (43.75%), Postives = 37/64 (57.81%), Query Frame = 3
Query:  129 CVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            C++   GE  AP C+ P   G C AY PR+ ++     C+SF+YGGC  N NNF + E CE  C
Sbjct:  376 CMLACGGELAAP-CSLPSLQGPCKAYEPRWAYSSTLKKCQSFVYGGCGGNENNFESKEACEQMC 438          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 159.458 bits (402), Expect = 6.557e-52
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE
Sbjct:    1 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 77          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Match: SMESG000037995.1 (SMESG000037995.1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.833e-27
Identity = 45/72 (62.50%), Postives = 54/72 (75.00%), Query Frame = 3
Query:  120 LIFCVIFSLG---ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            L+  V+FSL    ESR  ECNEPK++GMC AYF RFYFN ++G CE+FIYGGC  NGNNF T +ECE  C+E
Sbjct:    8 LVLVVLFSLSQLLESRLKECNEPKQSGMCMAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 79          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 97.0561 bits (240), Expect = 3.194e-27
Identity = 45/72 (62.50%), Postives = 54/72 (75.00%), Query Frame = 3
Query:  120 LIFCVIFSLG---ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            L+  V+FSL    ESR  ECNEPK++GMC AYF RFYFN ++G CE+FIYGGC  NGNNF T +ECE  C+E
Sbjct:    8 LVLVVLFSLSQLLESRLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 79          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.298e-27
Identity = 45/72 (62.50%), Postives = 54/72 (75.00%), Query Frame = 3
Query:  120 LIFCVIFSLG---ESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 326
            L+  V+FSL    ESR  ECNEPK++GMC AYF RFYFN ++G CE+FIYGGC  NGNNF T +ECE  C+E
Sbjct:    6 LVLVVLFSLSQLLESRLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 77          
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Match: SMESG000038052.1 (SMESG000038052.1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.483e-27
Identity = 41/75 (54.67%), Postives = 55/75 (73.33%), Query Frame = 3
Query:   96 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 320
            MNS+VIF+ IF  +  L ES++PECN P +TG+C AYF R+ F+++ G C +F+YGGC  N NNF+T  ECE RC
Sbjct:    1 MNSLVIFSCIFVAVLQLTESKSPECNLPLETGLCEAYFVRYGFDVKLGKCRTFVYGGCHVNDNNFHTRAECEERC 75          
The following BLAST results are available for this feature:
BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WFIKKN22.623e-1452.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
WFIKKN23.632e-1452.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
APLP23.235e-1147.06amyloid beta precursor like protein 2 [Source:HGNC... [more]
APLP23.240e-1147.06amyloid beta precursor like protein 2 [Source:HGNC... [more]
APLP23.241e-1147.06amyloid beta precursor like protein 2 [Source:HGNC... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mlt-116.346e-1346.43pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-117.057e-1346.43pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-117.328e-1346.43pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-111.073e-1246.43pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-111.242e-1246.43pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG36048.937e-1549.06gene:FBgn0031562 transcript:FBtr0077545[more]
CG36048.937e-1549.06gene:FBgn0031562 transcript:FBtr0346581[more]
CG154186.869e-1346.43gene:FBgn0031554 transcript:FBtr0077556[more]
CG154186.869e-1346.43gene:FBgn0031554 transcript:FBtr0446100[more]
Ppn6.019e-1148.08gene:FBgn0003137 transcript:FBtr0301839[more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tfpia1.633e-1253.85tissue factor pathway inhibitor a [Source:ZFIN;Acc... [more]
tfpia1.829e-1253.85tissue factor pathway inhibitor a [Source:ZFIN;Acc... [more]
spint21.323e-1152.83serine peptidase inhibitor, Kunitz type, 2 [Source... [more]
spint21.361e-1152.83serine peptidase inhibitor, Kunitz type, 2 [Source... [more]
appb5.085e-1147.06amyloid beta (A4) precursor protein b [Source:ZFIN... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WFIKKN24.197e-1256.86WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
tfpi24.908e-1144.62tissue factor pathway inhibitor 2 [Source:NCBI gen... [more]
spint23.856e-1046.30serine peptidase inhibitor, Kunitz type, 2 [Source... [more]
ENSXETT00000011536.14.699e-1048.94pep primary_assembly:Xenopus_tropicalis_v9.1:KV462... [more]
APLP24.908e-1045.10amyloid beta (A4) precursor-like protein 2 [Source... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Wfikkn24.760e-1452.94WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
Spint52.390e-1252.83serine protease inhibitor, Kunitz type 5 [Source:M... [more]
Spint52.776e-1252.83serine protease inhibitor, Kunitz type 5 [Source:M... [more]
Spint22.842e-1247.06serine protease inhibitor, Kunitz type 2 [Source:M... [more]
Spint34.599e-1247.37serine peptidase inhibitor, Kunitz type, 3 [Source... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0DMJ5|VKT_HETCR3.663e-1848.68PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=17577... [more]
sp|P83606|BMTI6_RHIMP1.030e-1762.07Kunitz-type serine protease inhibitor 6 (Fragment)... [more]
sp|B2G331|VKT1_HETCR1.177e-1647.37TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=17... [more]
sp|C0HK74|VKT3_HETMG1.352e-1660.78Kunitz-type serine protease inhibitor HMGS2 OS=Het... [more]
sp|P0DN09|VKT5_ANEVI1.828e-1646.48U-actitoxin-Avd3h OS=Anemonia viridis OX=51769 PE=... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C1IBY42.337e-1949.33Tabserpin OS=Tabanus yao OX=485572 PE=2 SV=1[more]
C8YJ955.643e-1949.33Serine protease inhibitor 3 OS=Tabanus yao OX=4855... [more]
C8YJ948.532e-1948.00Serine protease inhibitor 2 OS=Tabanus yao OX=4855... [more]
C8YJ973.547e-1849.33Serine protease inhibitor 5 OS=Tabanus yao OX=4855... [more]
C8YJ961.031e-1646.67Serine protease inhibitor 4 OS=Tabanus yao OX=4855... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000015806.26.005e-1358.33tissue factor pathway inhibitor-like [Source:NCBI ... [more]
paplnb1.265e-1250.00papilin-like [Source:NCBI gene;Acc:103035042][more]
paplnb1.772e-1250.00papilin-like [Source:NCBI gene;Acc:103035042][more]
wfikkn2b1.924e-1246.88WAP, Kazal, immunoglobulin, Kunitz and NTR domain-... [more]
lrp112.207e-1141.51LDL receptor related protein 11 [Source:NCBI gene;... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tfpi21.887e-1551.61tissue factor pathway inhibitor 2 [Source:ZFIN;Acc... [more]
ENSPMAT00000005038.14.608e-1052.27pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
ENSPMAT00000005043.15.262e-1052.27pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
WFIKKN12.658e-935.00WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
ENSPMAT00000008261.15.316e-839.22pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO298196.504e-1859.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO416963.617e-1553.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO262633.617e-1553.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382841.925e-1451.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO419223.827e-1343.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000012975.25.266e-1250.00pep primary_assembly:ASM223467v1:12:15740273:15760... [more]
spint1a7.367e-1240.24kunitz-type protease inhibitor 1 [Source:NCBI gene... [more]
spint1a6.786e-1141.18serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
spint1a7.365e-1141.18serine peptidase inhibitor, Kunitz type 1 [Source:... [more]
wfikkn2a1.245e-1043.75WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
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BLAST of Kunitz-type serine protease inhibitor BmKTT-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000037994.16.557e-52100.00SMESG000037994.1[more]
SMESG000037995.12.833e-2762.50SMESG000037995.1[more]
SMESG000037999.13.194e-2762.50SMESG000037999.1[more]
SMESG000037999.13.298e-2762.50SMESG000037999.1[more]
SMESG000038052.13.483e-2754.67SMESG000038052.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026393 ID=SMED30026393|Name=Kunitz-type serine protease inhibitor BmKTT-2|organism=Schmidtea mediterranea sexual|type=transcript|length=419bp
AATCATCTGCGAGTTCACTTAATAATTATAAAATATTTGATTACAAATTA
ACTATAAAATATAATTCAATCCAAATTTTTATAATCAAACCAAAAATGAA
TTCAATTGTAATATTCACTTTGATTTTTTGTGTAATCTTTAGTTTGGGAG
AAAGTCGAGCACCCGAATGTAACGAACCAAAGAAGACAGGAATGTGCGCT
GCTTATTTTCCAAGATTTTATTTCAATATTCAATCGGGAAATTGTGAATC
ATTTATTTATGGTGGATGCCAAGCTAATGGAAACAATTTTAACACAATTG
AAGAGTGTGAAGCTCGTTGTTTGGAATAGTTACAAACTTTTGAAATTTCA
AAAATAAAATAATAAACAACTATGAGTTTATTTTATAAAAAGATATTTCA
AAATAAAGCGAAATTAATT
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protein sequence of SMED30026393-orf-1

>SMED30026393-orf-1 ID=SMED30026393-orf-1|Name=SMED30026393-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
SSASSLNNYKIFDYKLTIKYNSIQIFIIKPKMNSIVIFTLIFCVIFSLGE
SRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIE
ECEARCLE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
GO:0033644host cell membrane
Vocabulary: biological process
TermDefinition
GO:0010466negative regulation of peptidase activity
GO:0044562envenomation resulting in negative regulation of voltage-gated potassium channel activity in other organism
GO:0006508proteolysis
GO:0010951negative regulation of endopeptidase activity
Vocabulary: molecular function
TermDefinition
GO:0030414peptidase inhibitor activity
GO:0090729toxin activity
GO:0004867serine-type endopeptidase inhibitor activity
GO:0008233peptidase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000067goblet cell
PLANA:0000240body wall musculature
PLANA:0000419prepharyngeal region
PLANA:0000421tail
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR036880Kunitz_BPTI_sf
IPR002223Kunitz_BPTI
IPR020901Prtase_inh_Kunz-CS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 108..108
NoneNo IPR availablePANTHERPTHR10083KUNITZ-TYPE PROTEASE INHIBITOR-RELATEDcoord: 53..106
IPR002223Pancreatic trypsin inhibitor Kunitz domainPRINTSPR00759BASICPTASEcoord: 91..106
score: 45.48
coord: 53..67
score: 30.21
coord: 81..91
score: 55.77
IPR002223Pancreatic trypsin inhibitor Kunitz domainSMARTSM00131KU_2coord: 54..107
e-value: 1.0E-21
score: 88.2
IPR002223Pancreatic trypsin inhibitor Kunitz domainPFAMPF00014Kunitz_BPTIcoord: 56..106
e-value: 9.4E-18
score: 64.2
IPR002223Pancreatic trypsin inhibitor Kunitz domainPROSITEPS50279BPTI_KUNITZ_2coord: 56..106
score: 15.129
IPR002223Pancreatic trypsin inhibitor Kunitz domainCDDcd00109KUcoord: 56..107
e-value: 1.53994E-21
score: 78.4458
IPR036880Pancreatic trypsin inhibitor Kunitz domain superfamilyGENE3DG3DSA:4.10.410.10coord: 52..108
e-value: 9.0E-22
score: 78.6
IPR036880Pancreatic trypsin inhibitor Kunitz domain superfamilySUPERFAMILYSSF57362BPTI-likecoord: 51..107
IPR020901Proteinase inhibitor I2, Kunitz, conserved sitePROSITEPS00280BPTI_KUNITZ_1coord: 84..102