carboxypeptidase B-like

Overview
Namecarboxypeptidase B-like
Smed IDSMED30026378
Length (bp)1378
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of carboxypeptidase B-like (SMED30026378) t-SNE clustered cells

Violin plots show distribution of expression levels for carboxypeptidase B-like (SMED30026378) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of carboxypeptidase B-like (SMED30026378) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for carboxypeptidase B-like (SMED30026378) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30026378

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30026378h1SMcG0017813 SmedASXL_008629SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30026378h1SMcG0017813 SmedASXL_008629SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30026378h1SMcG0017813 dd_Smed_v6_2541_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30026378h1SMcG0017813 OX_Smed_1.0.19549ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30026378h1SMcG0017813 Contig5714uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30026378h1SMcG0017813 Contig5714newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of carboxypeptidase B-like vs. Ensembl Human
Match: CPA2 (carboxypeptidase A2 [Source:HGNC Symbol;Acc:HGNC:2297])

HSP 1 Score: 234.187 bits (596), Expect = 8.807e-72
Identity = 139/396 (35.10%), Postives = 216/396 (54.55%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIEL--SQKLKLDLWKHPSIGQPVKFSTESDAEHV-------TYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + E+ P+NE+Q+ + ++L   + L+LD WK P+        T  +  HV         ++  L    +   I+ E++   + KE   NE    + + + +G      Y    +E +  + +L  ++   +S VNIG S+E + MNV+K STG  K  I++D GIHAREW+  ++ +W  NK+VS +   +  +  IL+  +I++LP+TNPDGY  ++   K+R+WRK    V    C+GVD NRN+DA FG  G SS+PC+  Y G  A    E  +  DF+ S   K+K F++ H+YSQLL+ P+ Y  T   D DE+  VA   A S+    G +Y VG   +++Y ASGG  DW    G+KYS+ FE+R  +  R GF+ P   I P  +E +
Sbjct:   27 VLEIVPSNEEQIKNLLQLEAQEHLQLDFWKSPT--------TPGETAHVRVPFVNVQAVKVFLESQGIAYSIMIEDVQVLLDKE---NEEMLFNRRRERSGNFNFGAYHT-LEEISQEMDNLVAEHPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIVSDYG-KDPSITSILDALDIFLLPVTNPDGYVFSQT--KNRMWRKTRSKVSGSLCVGVDPNRNWDAGFGGPGASSNPCSDSYHGPSANSEVEVKSIVDFIKS-HGKVKAFITLHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLRSLHGTKYKVGPICSVIYQASGGSIDWSYDYGIKYSFAFELR--DTGRYGFLLPARQILPTAEETW 404          
BLAST of carboxypeptidase B-like vs. Ensembl Human
Match: CPO (carboxypeptidase O [Source:HGNC Symbol;Acc:HGNC:21011])

HSP 1 Score: 202.601 bits (514), Expect = 3.680e-60
Identity = 111/306 (36.27%), Postives = 174/306 (56.86%), Query Frame = 2
Query:  401 KAAGAAGIETY----FAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLS--TGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            K+     +ETY    + P  E   W++ +S++  + ++   +G +YE   M  +K+S  +G  K+ I++D GIHAREW+  +   W + +++   K +N+ ++ +L   + Y+LP+ N DGY  T     DRLWRK+  P  NG C G DLNRNF+A +   G S +  +  + G+     PET     F+ S KD +  F++ H+Y QL+LTP+ YT+   S++ EM++V    A+++  K G  Y VGS A+ILYA+SG   DW + IG+ +SYTFE+R  +    GFV P   I P  +E  E
Sbjct:   35 KSVSPWSLETYSYNIYHPMGEIYEWMREISEKYKEVVTQHFLGVTYETHPMYYLKISQPSGNPKKIIWMDCGIHAREWIAPAFCQWFVKEILQNHK-DNSSIRKLLRNLDFYVLPVLNIDGYIYTWT--TDRLWRKSRSPHNNGTCFGTDLNRNFNASWCSIGASRNCQDQTFCGTGPVSEPETKAVASFIESKKDDILCFLTMHSYGQLILTPYGYTKNKSSNHPEMIQVGQKAANALKAKYGTNYRVGSSADILYASSGSSRDWARDIGIPFSYTFELR--DSGTYGFVLPEAQIQPTCEETME 335          
BLAST of carboxypeptidase B-like vs. Ensembl Human
Match: CPA5 (carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:15722])

HSP 1 Score: 201.06 bits (510), Expect = 6.537e-59
Identity = 127/396 (32.07%), Postives = 205/396 (51.77%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPS-----IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKE-TIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  V   +EKQL+   +L   +  K+D W+ P+     +   V FS   D +   Y+ SH    ++ ++ +   LD+      K   ++R  + F  +    +E       E  +W+ +   ++S  +S + IG S+E + + V+K STG  +   I+ID GIH+REW+  ++ +W  NK+VS +  +   + DILN  +I+I  +TNPDG+  T     +RLWRKN   +  G  C+GVDLNRN+ + FG  G +S+PC+  Y G      PE     +F+ +     K  +S H+YSQ+L+ P+     P S+  E+  +A    +++YK  G  YI GS +  LY ASG   DW    G+KY+++FE+R   Q   GF+ P   I P   E +
Sbjct:   42 VLRVLAKDEKQLSLLGDLEGLKPQKVDFWRGPARPSLPVDMRVPFSELKDIK--AYLESHGLAYSIMIKDIQVLLDEERQAMAKSRRLERSTNSFSYSSYHTLE-------EIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTANKIVSDYGKDRV-LTDILNAMDIFIELVTNPDGFAFTHS--MNRLWRKNK-SIRPGIFCIGVDLNRNWKSGFGGNGSNSNPCSETYHGPSPQSEPEVAAIVNFI-TAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLAKDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQ--YGFLLPATQIIPTAQETW 421          
BLAST of carboxypeptidase B-like vs. Ensembl Human
Match: CPA5 (carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:15722])

HSP 1 Score: 201.06 bits (510), Expect = 6.537e-59
Identity = 127/396 (32.07%), Postives = 205/396 (51.77%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPS-----IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKE-TIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  V   +EKQL+   +L   +  K+D W+ P+     +   V FS   D +   Y+ SH    ++ ++ +   LD+      K   ++R  + F  +    +E       E  +W+ +   ++S  +S + IG S+E + + V+K STG  +   I+ID GIH+REW+  ++ +W  NK+VS +  +   + DILN  +I+I  +TNPDG+  T     +RLWRKN   +  G  C+GVDLNRN+ + FG  G +S+PC+  Y G      PE     +F+ +     K  +S H+YSQ+L+ P+     P S+  E+  +A    +++YK  G  YI GS +  LY ASG   DW    G+KY+++FE+R   Q   GF+ P   I P   E +
Sbjct:   42 VLRVLAKDEKQLSLLGDLEGLKPQKVDFWRGPARPSLPVDMRVPFSELKDIK--AYLESHGLAYSIMIKDIQVLLDEERQAMAKSRRLERSTNSFSYSSYHTLE-------EIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTANKIVSDYGKDRV-LTDILNAMDIFIELVTNPDGFAFTHS--MNRLWRKNK-SIRPGIFCIGVDLNRNWKSGFGGNGSNSNPCSETYHGPSPQSEPEVAAIVNFI-TAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLAKDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQ--YGFLLPATQIIPTAQETW 421          
BLAST of carboxypeptidase B-like vs. Ensembl Human
Match: CPA5 (carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:15722])

HSP 1 Score: 201.06 bits (510), Expect = 6.537e-59
Identity = 127/396 (32.07%), Postives = 205/396 (51.77%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPS-----IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKE-TIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  V   +EKQL+   +L   +  K+D W+ P+     +   V FS   D +   Y+ SH    ++ ++ +   LD+      K   ++R  + F  +    +E       E  +W+ +   ++S  +S + IG S+E + + V+K STG  +   I+ID GIH+REW+  ++ +W  NK+VS +  +   + DILN  +I+I  +TNPDG+  T     +RLWRKN   +  G  C+GVDLNRN+ + FG  G +S+PC+  Y G      PE     +F+ +     K  +S H+YSQ+L+ P+     P S+  E+  +A    +++YK  G  YI GS +  LY ASG   DW    G+KY+++FE+R   Q   GF+ P   I P   E +
Sbjct:   42 VLRVLAKDEKQLSLLGDLEGLKPQKVDFWRGPARPSLPVDMRVPFSELKDIK--AYLESHGLAYSIMIKDIQVLLDEERQAMAKSRRLERSTNSFSYSSYHTLE-------EIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTANKIVSDYGKDRV-LTDILNAMDIFIELVTNPDGFAFTHS--MNRLWRKNK-SIRPGIFCIGVDLNRNWKSGFGGNGSNSNPCSETYHGPSPQSEPEVAAIVNFI-TAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLAKDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQ--YGFLLPATQIIPTAQETW 421          
BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Match: Y18H1A.9 (pep chromosome:WBcel235:I:748228:757010:-1 gene:WBGene00021213.1 transcript:Y18H1A.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y18H1A.9)

HSP 1 Score: 217.624 bits (553), Expect = 4.031e-65
Identity = 139/417 (33.33%), Postives = 224/417 (53.72%), Query Frame = 2
Query:  122 ESRMGLFEVTPTNEKQLNDFIELS-QKLKLDLWKHPS-IGQPVK--FSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLST---GPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKD--RLWRKNTMP--------------VENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAY-TQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            + +  ++ V PT + Q++    L    +KLD WK PS  G+ V    S E   + +  +  H  + ++ +  V + + +   K  K  +  R+H   +      +  Y +   +  N+L SL+    + +SV  IG ++EG+ + +IK++    G  K  I++DGGIHAREW+  S+V++ +++LV+ +   +  +K  +++ E YI+P+ NPDGYE +R       RLWRKN  P                  C GVDLNRNFD  FG+ G S+DPC+ +Y G+ AF  PET   RDFV   + ++  F++FH+YSQ+L+ P+ +  +T  +D +++   A   A ++      +Y VG+ A+ LY ASGG  DW K K  VKYS+ FE+RP+ Q   GF+     I P   E +E
Sbjct:   18 QKKFTVWRVQPTTQDQVDILHNLYLNDVKLDFWKSPSEAGKEVHVMISDEKTQQFMKELDDHSIKHSVMIDDVQKVIVEQKEKRDKLRKQVRLHDWREQKVQFNLAQYHS-FADVINYLNSLAITYPELVSVQPIGTTHEGRQIPLIKITNKRNGGTKRGIWVDGGIHAREWVSPSTVLYFIHQLVTQYD-KDVQIKQFVDQLEWYIVPLLNPDGYEYSRSSNDPEIRLWRKNRSPPKCIQQATGLFQPPTTTCCQGVDLNRNFDWFFGQVGSSTDPCSEIYQGAYAFSEPETAAVRDFVQ--RHRISTFLTFHSYSQILMYPFGHQVRTYSNDLNDLRSTALSAAQALNSVYNTQYKVGTGADTLYPASGGSEDWAKGKAHVKYSFLFELRPEEQVWDGFLLAENQIIPTARETWE 430          
BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Match: Y47G6A.19 (pep chromosome:WBcel235:I:3523334:3529956:-1 gene:WBGene00021645.1 transcript:Y47G6A.19b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y47G6A.19)

HSP 1 Score: 202.986 bits (515), Expect = 1.069e-59
Identity = 124/412 (30.10%), Postives = 209/412 (50.73%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKL---KLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENL-------DDTISKEIKDNE--VKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLST-GPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRK---GGKDRLWRKNTMP-------VENGC-LGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQ-TPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKK-IGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +F V PT  +QL   I L +     + D W  PS+            EH    R +L +     ++  ++L       +  +S    D+   +KR+H          +  Y++ +   + WL+ +++       ++ +G + EG+D+  +K     P K+ + ID GIHAREW  + +  + +N +V+G +  +  +++ L+   +YI+P+ NPDGYE TR      + R+WRK+  P       V N C +GVDLNRNFD  F   G S  PC+ +Y G  AF  PE+  +  F+ S+K +L+ +++ H+YSQL +  +++ + T   D +E  RVA      + +  G +Y  G+   I+YA SGG  DW K+ + +KYSYT E+RP  ++ +GFV     + P   E +
Sbjct:   25 VFRVLPTTNQQLLQMIRLFETADTDRADFWHAPSVVNGT-VDIMVAPEHTDQFRQYLEKHGYTFQVAIDDLHKLLIEKEGNLSTHSNDDAFFLKRLHDDVGFHSRLRMGEYYS-YSVLSTWLERIAENMPDIAKLIKVGTTIEGRDILGLKFGKDTPDKKIVVIDAGIHAREWAAIHTASYFINLIVNG-REEDPQIQNYLDNIVLYIIPVLNPDGYEYTRTDKTNPRARMWRKSRSPKACAFDGVRNSCCMGVDLNRNFDFRFSEIGASRYPCSEIYHGPSAFSEPESKAYSQFLTSLKGRLEAYITLHSYSQLWIYSYSHRKFTYAPDIEETRRVAAKAVQELGRMYGTKYRHGTGPEIIYAFSGGSTDWAKETLKIKYSYTIELRPGYEEWNGFVLDKNQLIPTAKETW 433          
BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Match: Y47G6A.19 (pep chromosome:WBcel235:I:3523313:3529956:-1 gene:WBGene00021645.1 transcript:Y47G6A.19a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y47G6A.19)

HSP 1 Score: 202.986 bits (515), Expect = 2.192e-59
Identity = 124/412 (30.10%), Postives = 209/412 (50.73%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKL---KLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENL-------DDTISKEIKDNE--VKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLST-GPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRK---GGKDRLWRKNTMP-------VENGC-LGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQ-TPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKK-IGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +F V PT  +QL   I L +     + D W  PS+            EH    R +L +     ++  ++L       +  +S    D+   +KR+H          +  Y++ +   + WL+ +++       ++ +G + EG+D+  +K     P K+ + ID GIHAREW  + +  + +N +V+G +  +  +++ L+   +YI+P+ NPDGYE TR      + R+WRK+  P       V N C +GVDLNRNFD  F   G S  PC+ +Y G  AF  PE+  +  F+ S+K +L+ +++ H+YSQL +  +++ + T   D +E  RVA      + +  G +Y  G+   I+YA SGG  DW K+ + +KYSYT E+RP  ++ +GFV     + P   E +
Sbjct:   25 VFRVLPTTNQQLLQMIRLFETADTDRADFWHAPSVVNGT-VDIMVAPEHTDQFRQYLEKHGYTFQVAIDDLHKLLIEKEGNLSTHSNDDAFFLKRLHDDVGFHSRLRMGEYYS-YSVLSTWLERIAENMPDIAKLIKVGTTIEGRDILGLKFGKDTPDKKIVVIDAGIHAREWAAIHTASYFINLIVNG-REEDPQIQNYLDNIVLYIIPVLNPDGYEYTRTDKTNPRARMWRKSRSPKACAFDGVRNSCCMGVDLNRNFDFRFSEIGASRYPCSEIYHGPSAFSEPESKAYSQFLTSLKGRLEAYITLHSYSQLWIYSYSHRKFTYAPDIEETRRVAAKAVQELGRMYGTKYRHGTGPEIIYAFSGGSTDWAKETLKIKYSYTIELRPGYEEWNGFVLDKNQLIPTAKETW 433          
BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Match: T06A4.1 (pep chromosome:WBcel235:I:770892:776369:1 gene:WBGene00020281.1 transcript:T06A4.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T06A4.1)

HSP 1 Score: 205.297 bits (521), Expect = 2.276e-59
Identity = 138/408 (33.82%), Postives = 225/408 (55.15%), Query Frame = 2
Query:  137 LFEVTPTNE---KQLNDFIELSQKLKLDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPK-----KETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--------CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAY-TQTPPSDNDEMMRVANLMA-DSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRS-GFVAPVEWISPAGDELYE 1297
            L  + P  E   K L    E     +LD W+ P+ IG  V   T + A+   +++   ++    +  VN+     +SK I++   +R   +F    A      +   +E    ++ L  +    I++++IG+S+E + + V+K+ TG +     K +++ID GIHAREW+  ++ ++I ++L+ G++ N+A V  +++  + YILP+ NPDGYE +R+  K+R+WRKN  P +          C GVDLNRNFD  +  TG SSDPC+  Y GS AF  PE+   RDF+   ++  + F+S H+YSQ+ L P+ +  Q+ P D    +R   L A  ++Y+  G +Y VG+ A+++Y ASGG +DW K ++ V Y+Y  E+RP N     GF+ P   I P G E +E
Sbjct:   34 LIRINPETEGSVKYLRSLYEDPSPYELDFWQPPTNIGAIVDL-TVAPADAPRFVKDLESKKISYIVAVND-----LSKAIEN---ERGSDKFYNPVAGFAYDKYNSLEEIQTEMKRLKKEYPTMITLIDIGQSHENRTLLVMKI-TGKRNPLGSKISMWIDAGIHAREWIAPATAMYIAHELLLGYE-NDATVAKLMDHIDFYILPVMNPDGYEYSRE--KNRMWRKNRSPAKCARQTFSTVCCSGVDLNRNFDWFWASTGSSSDPCHDTYHGSAAFSEPESQAVRDFLE--QNTPEAFISLHSYSQMWLIPYGHRKQSYPQDYHTGLRPLALRATKALYELYGTKYQVGTGADLMYEASGGSHDWAKGQLKVPYAYLIELRPKNTMMGHGFLLPEREIVPTGLETFE 426          
BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Match: ZC434.9 (pep chromosome:WBcel235:I:10356251:10360887:1 gene:WBGene00013895.1 transcript:ZC434.9b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC434.9)

HSP 1 Score: 195.282 bits (495), Expect = 2.207e-56
Identity = 125/407 (30.71%), Postives = 206/407 (50.61%), Query Frame = 2
Query:  164 KQLNDFIELSQKLKLDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNE-----------VKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG---PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKD--RLWRKNTMPV---------ENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLS--IKDKLKLFVSFHAYSQLLLTPWAYTQ-TPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            K L D  E +Q   LD W+ PS +G       + D   + ++ S L   N+   ++ E+    + K I + E            KR+H++       G   Y + ++   +W++ +  +      V  +G + EG+ +  IK+ +      K   +IDGGIHAREW  V + +W +++L++ +  +++ V+  ++R   YILP+ NPDGYE +R       RLWRKN   V         +  C GVDLNRN+D  FG TG S+D C+ +Y GS AF   ET + RDF+ S  +  K+  F++ H YSQ+ + P+++ + + P+D  E+ RV      ++    G +Y  G+ ++ILY ++GG  DW K  + +KY Y  E+RP      GF+     + P   E +
Sbjct:   47 KALRDIHERTQH-DLDFWQTPSKVGHRADIMVDED--RMEWLDSVLKNSNISYDVIIED----VGKLILEKEHGPPRFSNLLFSKRMHNEGGNRARYGFGEYHS-YQTICDWMKDIERKYPDKAKVFTMGTTSEGRPIQGIKIGSQVWRNDKRIFWIDGGIHAREWAAVHTALWFIDRLIADY-NDDSLVRAAVDRLNFYILPVANPDGYEYSRSDVSPMIRLWRKNRAGVVCKKDRWFRDRCCGGVDLNRNYDWHFGETGSSTDKCSEIYQGSSAFSESETRSMRDFLTSSELNGKIDAFITLHTYSQMWIHPYSHARKSVPADVAELQRVGRAAVAALENTYGTKYKFGTGSDILYPSAGGSDDWAKGTLRIKYVYLLELRPGEDVWDGFILDQHQLIPTAKETW 444          
BLAST of carboxypeptidase B-like vs. Ensembl Fly
Match: CG8560 (gene:FBgn0035781 transcript:FBtr0332725)

HSP 1 Score: 206.068 bits (523), Expect = 2.803e-61
Identity = 112/303 (36.96%), Postives = 169/303 (55.78%), Query Frame = 2
Query:  293 HLAE-LNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG---PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPS---DNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWV 1180
            HL E   ++  ++NEN  +++ +E  +N+ +R+ +   A  +   +  F  H E N +L  L+      +SV   GKSYE +D+  I ++ G     K  +F+D GIHAREW+  +  ++++++LV  F  N+  +KD    F+  ILP+ NPDGYE +      R+WRK   P+ + C G D NRNFD  +G  G SS  C+  + G  AF  PET   RD +LS+  + K +++ H+Y   LL PW +T   PS   DNDE   VA   AD+I   +G +Y VGS  N+LYAA+GG  D+ 
Sbjct:   76 HLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAERSVSFKA-FHRHAEINAYLDELAAAYPSRVSVQVAGKSYENRDIKTITITNGDGKTGKNVVFLDAGIHAREWIAHAGALYVIHQLVENFAANSELLKD----FDWVILPVVNPDGYEYSHT--TTRMWRKTRKPISSACYGTDANRNFDFHWGEVGASSYSCSDTFKGETAFSEPETQLIRDILLSLTGRGKFYLTLHSYGNYLLYPWGWTSALPSSWRDNDE---VAQGGADAIKSATGTKYTVGSSTNVLYAAAGGSDDYA 368          
BLAST of carboxypeptidase B-like vs. Ensembl Fly
Match: CG8560 (gene:FBgn0035781 transcript:FBtr0076868)

HSP 1 Score: 206.068 bits (523), Expect = 2.803e-61
Identity = 112/303 (36.96%), Postives = 169/303 (55.78%), Query Frame = 2
Query:  293 HLAE-LNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG---PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPS---DNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWV 1180
            HL E   ++  ++NEN  +++ +E  +N+ +R+ +   A  +   +  F  H E N +L  L+      +SV   GKSYE +D+  I ++ G     K  +F+D GIHAREW+  +  ++++++LV  F  N+  +KD    F+  ILP+ NPDGYE +      R+WRK   P+ + C G D NRNFD  +G  G SS  C+  + G  AF  PET   RD +LS+  + K +++ H+Y   LL PW +T   PS   DNDE   VA   AD+I   +G +Y VGS  N+LYAA+GG  D+ 
Sbjct:   76 HLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAERSVSFKA-FHRHAEINAYLDELAAAYPSRVSVQVAGKSYENRDIKTITITNGDGKTGKNVVFLDAGIHAREWIAHAGALYVIHQLVENFAANSELLKD----FDWVILPVVNPDGYEYSHT--TTRMWRKTRKPISSACYGTDANRNFDFHWGEVGASSYSCSDTFKGETAFSEPETQLIRDILLSLTGRGKFYLTLHSYGNYLLYPWGWTSALPSSWRDNDE---VAQGGADAIKSATGTKYTVGSSTNVLYAAAGGSDDYA 368          
BLAST of carboxypeptidase B-like vs. Ensembl Fly
Match: CG8562 (gene:FBgn0035779 transcript:FBtr0076854)

HSP 1 Score: 202.216 bits (513), Expect = 7.603e-60
Identity = 126/391 (32.23%), Postives = 197/391 (50.38%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQK-----LKLDLWKHPS--IGQPVK---FSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG---PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMP---VENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPV 1261
            ++EV P+   Q +   +LS +      +  L  HPS  I  P +   F    +AE +T            L +VN NL  +I++E    +++R+ +     G    E Y+  H+E  N++  L+ +    + V  +G SYE + +  I ++ G     K+ IF+DGG HAREW+  ++V++++++LV  F+ N   +KD    ++  ILP+ N DGYE T+ G   R+WRK   P       C G D NRNFD  +   G SS+PC   Y G  AF  PETI  RD + S+ D+  ++++ H+Y   LL PW +T   P + +++  VA   A++I   +G  Y  GS  N+LY A+G   D+    G   S T E+        GF  PV
Sbjct:   28 IYEVIPSTADQADLLHQLSLQGYDFISETRLLGHPSRVIVSPAQLKHFQQLVEAEKMT------------LTLVNSNLGASIAEEFAQRQMQRLLAPITGKGRLSTERYYT-HEEIINYIDDLAQRFPSRVFVKTVGWSYEQRVLKTITITNGDGKANKKVIFMDGGFHAREWISPAAVLYVIDQLVEQFEENAHLLKD----YDWVILPLVNADGYEHTQTGTLARMWRKTRQPYTYAGQTCYGADPNRNFDFHWNEEGASSNPCADTYAGPTAFSEPETITVRDLMHSLADRGIMYLTLHSYGNYLLYPWGWTSDLPENWEDLDAVARTGAEAIENATGTVYTYGSSTNVLYIAAGASDDYGYYAGFNVSITMEL--PGAGSIGFNPPV 399          
BLAST of carboxypeptidase B-like vs. Ensembl Fly
Match: CG18417 (gene:FBgn0035780 transcript:FBtr0076855)

HSP 1 Score: 200.675 bits (509), Expect = 3.532e-59
Identity = 106/321 (33.02%), Postives = 177/321 (55.14%), Query Frame = 2
Query:  269 EHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGP---KKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVG-SPANILYAASGGCYDWVKKIGVKYSYTFEV 1219
            E V +++  + E N+   I+N N+  T+S++ + N + R    F   G  G E Y+  H+E N +++ L+ ++ + + +  +G+SYEG+ +  I ++ G     K  I +DGG HAREW+  ++  +++N+LV   + N     D+L  F+  ILP+ NPDGYE T+     R+WRK   P  + C+G D NRNFD  +   G S DPC+ +Y G++ F  PE +   D + S  D+ +++++ H+Y  L+L PW +T   P   +++  VA     +I + +G  Y +G S   I YAASG   D+    G   S+T E+
Sbjct:   76 EQVNFVQL-MEENNVKYDIINRNVGLTLSRQFETNRMLR--HWFPYNGRLGTERYYN-HEEINQFIEDLAGEHPRRVFLKTVGRSYEGRWLKTITITNGDARRNKNVILVDGGFHAREWISPAAATYLINQLVYNLEDN----ADLLLDFDWVILPVVNPDGYEYTQLSEDTRMWRKTRKPSSSDCIGTDPNRNFDFHWNEEGASDDPCDNIYAGAKPFSEPEALVVGDLIHSYVDRGQMYLTLHSYGSLILYPWGWTAAVPDTEEDLHEVAAAGQSAIQEATGTIYKIGPSATTINYAASGASDDYAFNAGFPISFTMEL 388          
BLAST of carboxypeptidase B-like vs. Ensembl Fly
Match: CG3097 (gene:FBgn0029804 transcript:FBtr0070836)

HSP 1 Score: 196.823 bits (499), Expect = 1.607e-57
Identity = 124/386 (32.12%), Postives = 208/386 (53.89%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDA-EHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDN-EVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVV---NIGKSYEGKDMNVIKLSTGPKK-ETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWI 1270
            ++ ++   +K++     L QK   ++WK  S     KF   S A + +   RS L+   LD +I++ N+   I +E+ +  +        +   AA    ++  + +       + +  +KF ++V    IG++ EG+D+ V+++S  P++ + ++IDGGIHAREW+  ++V +IL +L+S ++   A ++ +      YI+P+ NPDGYE +R    +RLWRKN  P     C GVDLNRNFD  +   G S++PC+  Y GS      ET    +F+   K  L+ +++FH+Y Q+++ PWAY      D   + RV++L A+ I +K+G  Y       +L  A GG  DW +  +GVKY YT E+R  ++   GFV P  +I
Sbjct:   49 IYNISEAMQKRVPVGQMLEQKFGGNIWKENS-----KFLDISIARDQLKAARSFLSAHRLDPQILSHNIQSMIDEELLEGIQSSSFGHGRRTKKAARSSMHWKDYHDLETIYSFMREIRTKFPNIVRLYTIGQTAEGRDLKVLRISENPRENKKVWIDGGIHAREWISPATVTFILYQLMSDWENQPAHIRGLT----WYIMPVMNPDGYEYSRT--TNRLWRKNRSPSRRAQCSGVDLNRNFDIGWNGYGSSTNPCSDTYRGSAPASERETRAVAEFLAKRKYNLESYLTFHSYGQMIVYPWAYKAVKVKDASVLQRVSSLAAERILQKTGTSYRAAVTHEVLGIAGGGSDDWSRAALGVKYVYTIELR--DRGAYGFVLPPRFI 421          
BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Match: cpa5 (carboxypeptidase A5 [Source:ZFIN;Acc:ZDB-GENE-020514-1])

HSP 1 Score: 222.631 bits (566), Expect = 1.881e-67
Identity = 143/395 (36.20%), Postives = 207/395 (52.41%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQK---LKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAG-----IETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +F +TP +E Q++   EL+++   L L +W  P I + +          +  +R+ LA   +   I+ EN+ + +  E +D          K  G A      + T +      N+++  L  +N   +S V IG+SYE + +NV+K STG  +  I+ID GIH+REW+  +S VW   K+V  +  +   + DILN  +I++  +TNPDG+  T    KDR+WRK   P   + C+GVD NRN+DA FG  G S++PC   Y G  A   PE     DFV S   K+K FVS H+YSQ+LL P+ YT T   D  E+  VA     S+      RY  GS    +Y ASGG  DW    G+KYSYTFE+R  +  R GF+ P   I P  +E +
Sbjct:   25 VFRITPKDEAQIDLLKELTEEGEHLGLSVWMEP-ILESLPLDLHVPFHSLQAVRAFLAYNQIPYHIMIENVQELLDDEQRD--------MVKYRGLARSTDDFVYTTYHDLNSINSFMDMLVAENRNMVSKVVIGQSYEKRPLNVLKFSTGANRPGIWIDTGIHSREWVTQASGVWFAKKIVKDYGRDPV-LTDILNSHDIFLEIVTNPDGFVYTHT--KDRMWRKTRKPNPGSSCVGVDPNRNWDAGFGGGGSSNNPCTETYRGPSAHSEPEVKAIVDFVKS-HGKIKAFVSIHSYSQMLLYPYGYTYTAAKDKAELHEVARKAITSLQSLYNTRYTYGSIITTIYQASGGTIDWTYNQGIKYSYTFELR--DTGRYGFILPANQIVPTAEETW 404          
BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Match: cpb1 (carboxypeptidase B1 (tissue) [Source:ZFIN;Acc:ZDB-GENE-030131-1132])

HSP 1 Score: 221.861 bits (564), Expect = 2.774e-67
Identity = 126/391 (32.23%), Postives = 214/391 (54.73%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESD----AEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKL--STGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            + ++ P N++ +    EL +K+KLD WK P     V      D    A  +  + + L + +++++++ +NL + +  ++ +    + H   K    A I          N+W  S+S  N   IS  +IG +YEG+ M+++K+  +TG  K  +F+D G HAREW+  +   W +N+ VS + ++  D+ ++L+R + ++LP+ N DGYE T    +DR+WRK  +    + C+G D NRNF+A +   G SS+PC+  Y GS      E+ N  +F+ + K  +K +++ H+YSQLLL P++Y     + + E+M V+     ++    G +Y  G  A  +Y A+GG  DW   +GVKYSYTFE+R  ++ R GF+ P   I P  +E
Sbjct:   25 VLQLKPINDEHVTIIRELGEKIKLDFWK-PRSADLVSIDMTVDIHVPAAQLAMVSTILQQSDMEVKVMIDNLQEAVKGQMDNRSPTKGHDYTKYNSWATI----------NDWAISISSANPDLISRQSIGNTYEGRTMHLLKIGKNTGSNKPAVFMDCGFHAREWITHAFCQWFVNEAVSTYGSD-PDMTNLLDRMDFFVLPVFNIDGYEYTWN--RDRMWRKTRSKNSGSSCIGTDPNRNFNAGWCTVGASSNPCSDTYCGSSPESEIESKNLANFIRTNKSVIKAYLTVHSYSQLLLFPYSYKYDLAAHHSELMSVSQGAIAALRSLYGTKYTSGPGAATIYPAAGGSDDWAYDLGVKYSYTFELR--DEGRYGFLLPESQIKPTCEE 399          
BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Match: cpb1 (carboxypeptidase B1 (tissue) [Source:ZFIN;Acc:ZDB-GENE-030131-1132])

HSP 1 Score: 221.861 bits (564), Expect = 2.774e-67
Identity = 126/391 (32.23%), Postives = 214/391 (54.73%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESD----AEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKL--STGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            + ++ P N++ +    EL +K+KLD WK P     V      D    A  +  + + L + +++++++ +NL + +  ++ +    + H   K    A I          N+W  S+S  N   IS  +IG +YEG+ M+++K+  +TG  K  +F+D G HAREW+  +   W +N+ VS + ++  D+ ++L+R + ++LP+ N DGYE T    +DR+WRK  +    + C+G D NRNF+A +   G SS+PC+  Y GS      E+ N  +F+ + K  +K +++ H+YSQLLL P++Y     + + E+M V+     ++    G +Y  G  A  +Y A+GG  DW   +GVKYSYTFE+R  ++ R GF+ P   I P  +E
Sbjct:   25 VLQLKPINDEHVTIIRELGEKIKLDFWK-PRSADLVSIDMTVDIHVPAAQLAMVSTILQQSDMEVKVMIDNLQEAVKGQMDNRSPTKGHDYTKYNSWATI----------NDWAISISSANPDLISRQSIGNTYEGRTMHLLKIGKNTGSNKPAVFMDCGFHAREWITHAFCQWFVNEAVSTYGSD-PDMTNLLDRMDFFVLPVFNIDGYEYTWN--RDRMWRKTRSKNSGSSCIGTDPNRNFNAGWCTVGASSNPCSDTYCGSSPESEIESKNLANFIRTNKSVIKAYLTVHSYSQLLLFPYSYKYDLAAHHSELMSVSQGAIAALRSLYGTKYTSGPGAATIYPAAGGSDDWAYDLGVKYSYTFELR--DEGRYGFLLPESQIKPTCEE 399          
BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Match: cpa2 (carboxypeptidase A2 (pancreatic) [Source:ZFIN;Acc:ZDB-GENE-040801-182])

HSP 1 Score: 211.46 bits (537), Expect = 3.193e-63
Identity = 129/379 (34.04%), Postives = 198/379 (52.24%), Query Frame = 2
Query:  185 ELSQKLKLDLWKHP-SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKE----IKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPK-KETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGK--DRLWRKNTMPVEN-GCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            EL +   LD W H  S  +PV       + +   ++  L E N+   ++  N+ + + +E    + + E++R    F  A    ++T ++       ++ +L   +S  IS V IG +YE + M V+K STG + K  I+ID GIHAREW+  +S VWI +++ + FK N A  +++L++ +IY++ + NPDGY  +    +   RLWRK+     N  C GVDLNRNFDA+F   G S DPC   Y G  A    E  N  DF+LS     K F++ HAYSQLL+ P+ YT +   D  ++  VA     ++    G  Y VGS  + +  ASG   DW  + G+KY + FE+R  +    GF+ P + I     E +
Sbjct:   41 ELEKDSGLDFWTHGFSTERPVDVRVPHASLYA--VKDFLKENNISFSVMINNVQELLDRERAEMVSNTEMERNTKSFNFAAYHDLDTIYS-------FMDTLVASHSNLISKVKIGSTYENRPMYVLKFSTGGENKPAIWIDAGIHAREWVSHASAVWIADRIATDFKENRALARNVLSKMDIYLMIVANPDGYVFSHLTVRPFHRLWRKSRSVTSNPNCPGVDLNRNFDAEFSGPGASDDPCAEDYRGPSAHSEIEVTNIADFILS-HGNFKSFMTLHAYSQLLMYPYGYTGSNAPDQPDLHDVATQANKALNSWHGTIYKVGSIFHTIEQASGASVDWAYQHGIKYCFAFELR--DTGEYGFLLPADQIVSTARETW 407          
BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Match: cpa1 (carboxypeptidase A1 (pancreatic) [Source:ZFIN;Acc:ZDB-GENE-050522-438])

HSP 1 Score: 207.994 bits (528), Expect = 5.334e-62
Identity = 125/390 (32.05%), Postives = 202/390 (51.79%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQK--LKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRI-HSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMP-VENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  +   N +Q++   EL++   L LD W  P+  + +          +  +R+ LA   +   I+ EN+ D + +E    +++ + +  F  +    + T +      N+++  L  +N   +S + IG+SYE + +NV+K STG  +  I+ID G+H+REW+  +S +W   K+V+ +  +   + DILN ++I++  + NPDG   T     DR+WRK   P   + C+GVD NRN++  FG  G SS+PC+  Y G      PE     +FV S    LK F+S H+YSQ+LL P+ YTQT   D  E+  +A      +       Y  GS    +Y A G   DW  + G+KYSYTFE+R  +  R GF+ P + I P  +E +
Sbjct:   25 VLRINAKNAEQISLLKELTEADLLGLDFWMTPA-RESLPVDLHVPFHSLQAVRAFLAYNQIPYHIMIENVQDLVDEE----QLQMLKYRGFPRSTDDFVYTTYHDLDSINSFMDMLVAENQNMVSKIEIGQSYENRPLNVLKFSTGANRPGIWIDTGLHSREWITHASGIWFAKKIVTDYGRDPV-LTDILNSYDIFLEIVANPDGLAYTHS--DDRMWRKTRKPNPGSSCVGVDPNRNWETGFGGPGSSSNPCSQTYHGPSIHSEPEVKAIVEFVKS-HGNLKAFLSIHSYSQMLLYPYGYTQTAAKDQAELHELARKATSELQSLYNTAYTYGSIITTIYQADGVTTDWAYEQGIKYSYTFELR--DTGRYGFILPADQILPTAEETW 403          
BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Match: kpna1 (karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-1014184])

HSP 1 Score: 230.335 bits (586), Expect = 2.397e-70
Identity = 135/390 (34.62%), Postives = 206/390 (52.82%), Query Frame = 2
Query:  137 LFEVTPTNEKQLN--DFIELSQKLKLDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  + P++E Q+N    +E  + L+LD W  PS +  P+         H+  ++ +L   ++   I+  ++ + + +E +D    R     ++  +     Y     E N ++ +L  +N   +S + IG SYEG+ +NV+K STGP +   +ID GIH+REW+  +S VW   K+V+ F   +  + + LN+ +I I  +TNPDG+  T K   DR+WRK   P     C+G D NRN+DA FG  G SS+PC   Y G  A   PE     DFV S    +K FVS H+YSQ+LL P+ YT T   D+DE+  V+     ++    G  Y  GS  + +Y ASGG  DW    G+K+SY+FE+R  +  R GF  P   I P  +E +
Sbjct:   25 VLRIFPSDEAQVNLVRALEDEEYLQLDFWTSPSRVLLPIDIRVP--LHHLQSVKVYLESNSIQYSIMIHDVQNLLDEEQRDMRRNRASEDGRSTNSFNYAAYHT-FDEINAFIDNLVSENPNLVSKIQIGSSYEGRPLNVLKFSTGPNRPAFWIDTGIHSREWVTQASAVWFAKKIVTDFG-KDPSLTNTLNQMDILIEIVTNPDGFVYTHK--SDRMWRKTRSPNSGSLCVGTDPNRNWDAGFGGPGSSSNPCTETYRGRAAHSEPEVKAIVDFVKS-HGNIKGFVSIHSYSQMLLFPYGYTNTRVPDHDELNAVSKKAITALASLYGTAYTYGSIISTIYQASGGTIDWTYNQGIKHSYSFELR--DTGRYGFALPANQIIPTAEETW 405          
BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Match: kpna1 (karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-1014184])

HSP 1 Score: 225.713 bits (574), Expect = 7.955e-69
Identity = 135/388 (34.79%), Postives = 202/388 (52.06%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
               + P++E QL     L   L+LD W  PS +  P+         H+  ++ +L   ++   I+  ++ + + +E +D    R     ++  +     Y     E N ++ +L  +N   +S + IG SYEG+ +NV+K STGP +   +ID GIH+REW+  +S VW   K+V+ F   +  + + LN+ +I I  +TNPDG+  T K   DR+WRK   P     C+G D NRN+DA FG  G SS+PC   Y G  A   PE     DFV S    +K FVS H+YSQ+LL P+ YT T   D+DE+  V+     ++    G  Y  GS  + +Y ASGG  DW    G+K+SY+FE+R  +  R GF  P   I P  +E +
Sbjct:    3 FLRIFPSDEAQLVSHALLLLMLQLDFWTSPSRVLLPIDIRVP--LHHLQSVKVYLESNSIQYSIMIHDVQNLLDEEQRDMRRNRASEDGRSTNSFNYAAYHT-FDEINAFIDNLVSENPNLVSKIQIGSSYEGRPLNVLKFSTGPNRPAFWIDTGIHSREWVTQASAVWFAKKIVTDFG-KDPSLTNTLNQMDILIEIVTNPDGFVYTHK--SDRMWRKTRSPNSGSLCVGTDPNRNWDAGFGGPGSSSNPCTETYRGRAAHSEPEVKAIVDFVKS-HGNIKGFVSIHSYSQMLLFPYGYTNTRVPDHDELNAVSKKAITALASLYGTAYTYGSIISTIYQASGGTIDWTYNQGIKHSYSFELR--DTGRYGFALPANQIIPTAEETW 381          
BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Match: kpna1 (karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-1014184])

HSP 1 Score: 214.542 bits (545), Expect = 2.872e-65
Identity = 123/321 (38.32%), Postives = 174/321 (54.21%), Query Frame = 2
Query:  335 NLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            NL+D   K+ +D    R     ++  +     Y     E N ++ +L  +N   +S + IG SYEG+ +NV+K STGP +   +ID GIH+REW+  +S VW   K+V+ F   +  + + LN+ +I I  +TNPDG+  T K   DR+WRK   P     C+G D NRN+DA FG  G SS+PC   Y G  A   PE     DFV S    +K FVS H+YSQ+LL P+ YT T   D+DE+  V+     ++    G  Y  GS  + +Y ASGG  DW    G+K+SY+FE+R  +  R GF  P   I P  +E +
Sbjct:    1 NLEDEQKKQ-RDMRRNRASEDGRSTNSFNYAAYHT-FDEINAFIDNLVSENPNLVSKIQIGSSYEGRPLNVLKFSTGPNRPAFWIDTGIHSREWVTQASAVWFAKKIVTDFG-KDPSLTNTLNQMDILIEIVTNPDGFVYTHK--SDRMWRKTRSPNSGSLCVGTDPNRNWDAGFGGPGSSSNPCTETYRGRAAHSEPEVKAIVDFVKS-HGNIKGFVSIHSYSQMLLFPYGYTNTRVPDHDELNAVSKKAITALASLYGTAYTYGSIISTIYQASGGTIDWTYNQGIKHSYSFELR--DTGRYGFALPANQIIPTAEETW 313          
BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Match: znfx1 (zinc finger NFX1-type containing 1 [Source:Xenbase;Acc:XB-GENE-1218977])

HSP 1 Score: 203.371 bits (516), Expect = 1.218e-59
Identity = 132/390 (33.85%), Postives = 203/390 (52.05%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELN---LDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLS--TGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            +F++TP   +       L+ +  LDLW+     Q V+   E    HV    +++ ++    L   I  E L + + K I  N V     Q KA+      T + P  E  NW+  + +++S+ +S   IG +YE + M  +K+   +  +K+  FID G HAREW+ V+   W +N++VS +KT+ A + ++L + + Y+LP+ N DGY  T     +RLWRKN  P ENG C GVDLNRNFD+ +   G S D  +  + G  A   PET      +   K  +  +++ H+Y Q++L P+ Y + P  ++DEMM VA      + +K    Y   S A ILY  SG   DW  ++G++ SYT E+R DN    GFV P + I P  +E
Sbjct:   30 VFKITPETSEHAQYLQTLANEWLLDLWR----PQTVEQIHEGSDIHVQIPFAYMEQMKQKLLQHSIPYEVLINDVQKLIDSNTVSAPKIQ-KASLENYDYTKYHPMDEIYNWMDLMKEKHSEIVSQHYIGCTYELRPMYYLKIGWPSDKQKKIFFIDCGFHAREWISVAFCQWFVNEIVSHYKTD-AILANVLKQVDFYVLPVMNIDGYVYTWT--TNRLWRKNRSPHENGTCYGVDLNRNFDSQWCSIGASRDCNSNTFCGPEAASEPETKALSGLIEKTKSDILCYLTIHSYGQMILLPYGYKKDPSPNHDEMMLVAKNAVAKMKEKHNNEYEYESSAVILYYDSGSSGDWTVELGIQLSYTLELR-DN-GTYGFVLPPDQIKPTCEE 409          
BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Match: cpb2 (carboxypeptidase B2 [Source:NCBI gene;Acc:595005])

HSP 1 Score: 198.364 bits (503), Expect = 5.046e-58
Identity = 119/388 (30.67%), Postives = 207/388 (53.35%), Query Frame = 2
Query:  146 VTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELN----LDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPK--KETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            V P  ++Q+     ++ + ++ LWK  S    +K        + ++I +  AELN    L + +VN   +    +   D   +R  + F        + Y+        W+Q + +++S  +  ++IG S+E + + V+K+S   K  K  ++ID GIHAREW+  +  +W +   V  +  + +  K +L   + YILP+ N DGY+ +    K+R+WRKN +   ++ C+G DLNRNFDA +   G SSDPC+ +Y G  A   PE      F+   ++ +K +++ H+YSQ++L P++YT     D+DE++ ++  +A+ I   S  +Y+ G+ A  +Y A GG  DW   +G+KYS+TFE+R  ++   GF+ P + I P   E
Sbjct:   30 VLPRTDQQVEILKNITSQPEIVLWKPDSNEHIMKNKEVHFYVNESFIDTVKAELNESSILYMVLVNNAQELIEQQTFNDTSNQRSATSFYEQYHTLEDIYY--------WMQHMVEKHSDMLQRIHIGYSFENRPLYVLKVSGKEKTAKHAVWIDCGIHAREWISPAFCLWFVGHAVEYYGVDLSMTK-LLRYLDFYILPVMNADGYQFSWTA-KNRMWRKNRSKYTKSNCIGTDLNRNFDAGWCGPGASSDPCHEIYCGPYAESEPEVSAVVSFLKKHQNVVKGYITVHSYSQMVLFPYSYTNKKSKDHDELLLLSKKVAEGIRSTSRNKYMYGAGAETIYLAPGGSDDWAYDLGIKYSFTFELR--DKGMYGFLLPPQLIKPTCSE 405          
BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Match: Cpa2 (carboxypeptidase A2, pancreatic [Source:MGI Symbol;Acc:MGI:3617840])

HSP 1 Score: 232.261 bits (591), Expect = 3.540e-71
Identity = 139/397 (35.01%), Postives = 217/397 (54.66%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEV----KRIHSQFKAAGAAGIETYFAPH---KEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + EV P +E+Q+   ++L   + L+LD WK PS+                ++R   A +  D+++  E+   T S  I+D +V    +R    F      G    F  +   +E    + +L  +N   +S VNIG S+E + MNV+K STG  K  I++D GIHAREW+  ++ +W  NK+ S + T+ A +  +LN  ++++LP+TNPDGY  ++    +R+WRK T    +G  C+GVD NRN+DA+FG  G SS+PC+  Y G       E  +  DF+ S   K+K F++ H+YSQLL+ P+ Y    P D +E+  VA   A S+ +  G  Y VG   +++Y ASGG  DW   +G+KYS+ FE+R  +    GF+ P + I P  +E +
Sbjct:   25 VLEVIPNDEEQIKTLLQLEAEEHLELDFWKSPSV-----------PRQTVHVRVPFASIQ-DVKVFLESQGITYSIMIEDVQVLLDQEREEMLFNQQRERGTNFNFGAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPA-ITSLLNTLDVFLLPVTNPDGYVFSQT--SNRMWRK-TRSKRSGSFCVGVDPNRNWDANFGGPGASSNPCSDSYHGPSPNSEVEVKSIVDFIKS-HGKVKAFITLHSYSQLLMFPYGYKCAKPDDFNELDEVAQRAAQSLKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELR--DTGYYGFLLPAKQILPTAEETW 402          
BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Match: Cpa5 (carboxypeptidase A5 [Source:MGI Symbol;Acc:MGI:1921899])

HSP 1 Score: 204.527 bits (519), Expect = 2.359e-60
Identity = 124/395 (31.39%), Postives = 209/395 (52.91%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDF--IELSQKLKLDLWKHPS-----IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVEN-GCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  V   NEKQL+    +E  +  K+D W+ P+     +   V FS     +   Y++SH    ++ ++ +   LD+      K   ++R  + F  +    ++       E  +W+ +   ++S  +S ++IGKS+E + + V+K STG P +  I+ID GIH+REW+  ++ +WI  K+V+ +  ++  +K ILN  +I+I  +TNPDG+  T     +RLWRKN        C+GVDLNRN+ A FG  G + +PC+  Y G      PE     DF+       K  +S H+YSQ+++ P+ ++  P  +++E+  +A     ++ K  G +YI GS +  LY+ASG   DW    G+KY+++FE+R   Q   GF+ P   I P  +E +
Sbjct:   42 VLRVLAKNEKQLSLLRDLETQKPQKVDFWRGPARPSLPVDMRVPFSELPSVK--AYLKSHGLAYSIMIKDIQVLLDEERDAMAKSRRLERSTNSFSYSSYHTLD-------EIYSWIDNFVAEHSNLVSKIHIGKSFENRSILVLKFSTGGPNRPAIWIDTGIHSREWITHATGIWISQKIVNAYGKDHV-LKRILNTMDIFIEIVTNPDGFAFTHS--MNRLWRKNKSSQPGIFCIGVDLNRNWKAGFGGNGSNKNPCSETYRGPAPESEPEVAAIVDFITG-HGNFKAMISIHSYSQMVMYPYGHSLEPVPNHEELFNLAKDAVKALNKVHGIQYIFGSISTTLYSASGISVDWAYDSGIKYAFSFELRDTGQ--YGFLLPASQIVPTAEETW 421          
BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Match: Cpa5 (carboxypeptidase A5 [Source:MGI Symbol;Acc:MGI:1921899])

HSP 1 Score: 204.527 bits (519), Expect = 2.359e-60
Identity = 124/395 (31.39%), Postives = 209/395 (52.91%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDF--IELSQKLKLDLWKHPS-----IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVEN-GCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  V   NEKQL+    +E  +  K+D W+ P+     +   V FS     +   Y++SH    ++ ++ +   LD+      K   ++R  + F  +    ++       E  +W+ +   ++S  +S ++IGKS+E + + V+K STG P +  I+ID GIH+REW+  ++ +WI  K+V+ +  ++  +K ILN  +I+I  +TNPDG+  T     +RLWRKN        C+GVDLNRN+ A FG  G + +PC+  Y G      PE     DF+       K  +S H+YSQ+++ P+ ++  P  +++E+  +A     ++ K  G +YI GS +  LY+ASG   DW    G+KY+++FE+R   Q   GF+ P   I P  +E +
Sbjct:   42 VLRVLAKNEKQLSLLRDLETQKPQKVDFWRGPARPSLPVDMRVPFSELPSVK--AYLKSHGLAYSIMIKDIQVLLDEERDAMAKSRRLERSTNSFSYSSYHTLD-------EIYSWIDNFVAEHSNLVSKIHIGKSFENRSILVLKFSTGGPNRPAIWIDTGIHSREWITHATGIWISQKIVNAYGKDHV-LKRILNTMDIFIEIVTNPDGFAFTHS--MNRLWRKNKSSQPGIFCIGVDLNRNWKAGFGGNGSNKNPCSETYRGPAPESEPEVAAIVDFITG-HGNFKAMISIHSYSQMVMYPYGHSLEPVPNHEELFNLAKDAVKALNKVHGIQYIFGSISTTLYSASGISVDWAYDSGIKYAFSFELRDTGQ--YGFLLPASQIVPTAEETW 421          
BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Match: Cpa4 (carboxypeptidase A4 [Source:MGI Symbol;Acc:MGI:1919041])

HSP 1 Score: 200.675 bits (509), Expect = 3.884e-59
Identity = 129/395 (32.66%), Postives = 205/395 (51.90%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIEL--SQKLKLDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLD---DTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG--PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +F +   N  ++    EL  S  LKL +WK PS   +PV     S +  +  ++S L    LD  +  E+L    D   +E++ NE       F       +E  +  H+     + S++    + +S V IG+++E + M V+K STG   K+  I+++ GIHAREW+  ++ +W   K+V+ +K + A +  IL + +I++LP+ NPDGY  T+   ++RLWRK  +    + C+G D NRN++A F   G S +PC+ VY GS      E  +  DF+       K F+  H+YSQLL+ P+ YT     D +E+  VA   A ++   SG +Y VG     +Y ASG   DW    G+KY++TFE+R  +    GF+ P   I P  +E +
Sbjct:   26 VFRINVRNGDEIRKLTELVNSDHLKLSVWKSPSTFDRPVDILVPSVS--LLPVKSFLKSQGLDYSVTIEDLQALLDNEDEEMQHNEGIERSGDFNYGAYHPLEAIY--HE-----MDSIATDFPELVSRVKIGETFEKRPMYVLKFSTGGGKKRPAIWLNAGIHAREWISQATAIWTARKIVTDYKKDPA-ITSILKKVDIFLLPVANPDGYVYTQS--QNRLWRKTRSRNPGSRCVGADPNRNWNASFAGEGTSDNPCSEVYHGSHPNSEVEVKSVVDFIQK-HGNFKCFIDLHSYSQLLMYPYGYTVKKAPDAEELDDVARNAAQALASLSGTKYRVGPTCTTVYPASGSSVDWAYDNGIKYAFTFELR--DTGYYGFLLPASQIIPTAEETW 405          
BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Match: Cpa3 (carboxypeptidase A3, mast cell [Source:MGI Symbol;Acc:MGI:88479])

HSP 1 Score: 192.971 bits (489), Expect = 3.080e-56
Identity = 122/400 (30.50%), Postives = 211/400 (52.75%), Query Frame = 2
Query:  128 RMGLFEVTPTNEKQLNDFIELSQKLKLDLWKHP------SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKE--IKDNEVKRIHSQFKAAGAAGIETYFAPHKEYN---NWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGP-KKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            R  +F V   NEK  +    L+Q ++LD W +P      ++   V F   S+ E  T I+S L +  +   I+  +L + I K+  +KD E+   HS             +A + +++   +W + + +++ + +S + IG + E   + V+K+     +++ IF+D GIHAREW+  +   W + +    +  N    K +L+R   Y+LP+ N DGY       +DR+WRKN    +N  C+G DLNRNFD  +  +  ++ PC  VY G       ET    +F+ S  + +K +++FH+YSQ+LL P+ YT   P ++ ++++VA +  D++  +   RYI G  A+ +Y  SG   DWV  +G+K+++ FE+R  ++ +SGF+ P   I P   E
Sbjct:   23 REKVFRVKLQNEKHASVLKNLTQSIELDFW-YPDAIHDIAVNMTVDFRV-SEKESQT-IQSTLEQHKIHYEILIHDLQEEIEKQFDVKD-EIAGRHS-------------YAKYNDWDKIVSWTEKMLEKHPEMVSRIKIGSTVEDNPLYVLKIGKKDGERKAIFMDCGIHAREWISPAFCQWFVYQATKSYGKNKIMTK-LLDRMNFYVLPVFNVDGY--IWSWTQDRMWRKNRSRNQNSTCIGTDLNRNFDVSWDSSPNTNKPCLNVYRGPAPESEKETKAVTNFIRSHLNSIKAYITFHSYSQMLLIPYGYTFKLPPNHQDLLKVARIATDALSTRYETRYIYGPIASTIYKTSGSSLDWVYDLGIKHTFAFELR--DKGKSGFLLPESRIKPTCKE 400          
BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Match: sp|P19222|CBPA2_RAT (Carboxypeptidase A2 OS=Rattus norvegicus OX=10116 GN=Cpa2 PE=1 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 1.164e-72
Identity = 136/390 (34.87%), Postives = 220/390 (56.41%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPSI-GQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + E+ P++E+Q+   ++L   + L+LD WK P+I G+ V       +  +  ++  L    +D  I+ E++   + +E    E    + Q +  G    E Y    + Y   + +L  +N   +S VN+G S+E + MNV+K STG  K  I++D GIHAREW+  ++ +W  NK+ S + T+ A +  +LN  +I++LP+TNPDGY  ++    +R+WRK  +    +GC+GVD NRN+DA+FG  G SS PC+  Y G +     E  +  DF+ S   K+K F++ H+YSQLL+ P+ Y  T P D +E+  VA   A ++ +  G  Y VG   +++Y ASGG  DW   +G+KYS+ FE+R  +    GF+ P + I P  +E +
Sbjct:   25 VLEIIPSHEEQIRTLLQLEAEEHLELDFWKSPTIPGETVHVRVPFAS--IQAVKVFLESQGIDYSIMIEDVQVLLDQE---REEMLFNQQRERGGNFNFEAYHTLEEIYQE-MDNLVAENPGLVSKVNLGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPA-ITSLLNTLDIFLLPVTNPDGYVFSQT--TNRMWRKTRSKRSGSGCVGVDPNRNWDANFGGPGASSSPCSDSYHGPKPNSEVEVKSIVDFIKS-HGKVKAFITLHSYSQLLMFPYGYKCTKPDDFNELDEVAQKAAQALKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELR--DTAFYGFLLPAKQILPTAEETW 402          
BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Match: sp|P48052|CBPA2_HUMAN (Carboxypeptidase A2 OS=Homo sapiens OX=9606 GN=CPA2 PE=1 SV=3)

HSP 1 Score: 234.187 bits (596), Expect = 4.230e-71
Identity = 139/396 (35.10%), Postives = 216/396 (54.55%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIEL--SQKLKLDLWKHPSIGQPVKFSTESDAEHV-------TYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + E+ P+NE+Q+ + ++L   + L+LD WK P+        T  +  HV         ++  L    +   I+ E++   + KE   NE    + + + +G      Y    +E +  + +L  ++   +S VNIG S+E + MNV+K STG  K  I++D GIHAREW+  ++ +W  NK+VS +   +  +  IL+  +I++LP+TNPDGY  ++   K+R+WRK    V    C+GVD NRN+DA FG  G SS+PC+  Y G  A    E  +  DF+ S   K+K F++ H+YSQLL+ P+ Y  T   D DE+  VA   A S+    G +Y VG   +++Y ASGG  DW    G+KYS+ FE+R  +  R GF+ P   I P  +E +
Sbjct:   27 VLEIVPSNEEQIKNLLQLEAQEHLQLDFWKSPT--------TPGETAHVRVPFVNVQAVKVFLESQGIAYSIMIEDVQVLLDKE---NEEMLFNRRRERSGNFNFGAYHT-LEEISQEMDNLVAEHPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIVSDYG-KDPSITSILDALDIFLLPVTNPDGYVFSQT--KNRMWRKTRSKVSGSLCVGVDPNRNWDAGFGGPGASSNPCSDSYHGPSANSEVEVKSIVDFIKS-HGKVKAFITLHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLRSLHGTKYKVGPICSVIYQASGGSIDWSYDYGIKYSFAFELR--DTGRYGFLLPARQILPTAEETW 404          
BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Match: sp|Q504N0|CBPA2_MOUSE (Carboxypeptidase A2 OS=Mus musculus OX=10090 GN=Cpa2 PE=1 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 2.476e-70
Identity = 139/397 (35.01%), Postives = 217/397 (54.66%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEV----KRIHSQFKAAGAAGIETYFAPH---KEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + EV P +E+Q+   ++L   + L+LD WK PS+                ++R   A +  D+++  E+   T S  I+D +V    +R    F      G    F  +   +E    + +L  +N   +S VNIG S+E + MNV+K STG  K  I++D GIHAREW+  ++ +W  NK+ S + T+ A +  +LN  ++++LP+TNPDGY  ++    +R+WRK T    +G  C+GVD NRN+DA+FG  G SS+PC+  Y G       E  +  DF+ S   K+K F++ H+YSQLL+ P+ Y    P D +E+  VA   A S+ +  G  Y VG   +++Y ASGG  DW   +G+KYS+ FE+R  +    GF+ P + I P  +E +
Sbjct:   25 VLEVIPNDEEQIKTLLQLEAEEHLELDFWKSPSV-----------PRQTVHVRVPFASIQ-DVKVFLESQGITYSIMIEDVQVLLDQEREEMLFNQQRERGTNFNFGAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPA-ITSLLNTLDVFLLPVTNPDGYVFSQT--SNRMWRK-TRSKRSGSFCVGVDPNRNWDANFGGPGASSNPCSDSYHGPSPNSEVEVKSIVDFIKS-HGKVKAFITLHSYSQLLMFPYGYKCAKPDDFNELDEVAQRAAQSLKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELR--DTGYYGFLLPAKQILPTAEETW 402          
BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Match: sp|P04069|CBPB_ASTAS (Carboxypeptidase B OS=Astacus astacus OX=6715 PE=1 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.488e-65
Identity = 119/294 (40.48%), Postives = 166/294 (56.46%), Query Frame = 2
Query:  428 TYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVEN--GCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            T +  + E N WL SL+    +  SV ++G SYEG+ M ++KL  G   K  IFIDGGIHAREW+  S+V +I+N+ VS    N+A   DIL+    Y++P  NPDGY  T     DRLWRK      +  GC G D NRN+   +   G S  PC+ +Y G   F   E  N RD +L     +K++++FH+YSQL + PW +T   P D  ++  +A    D++    G RY +GS  N +YAA+GG  DW K + GVKY+YT E+R  +    GF+ P   I P G+E +E
Sbjct:    4 TSYHDYDEINAWLDSLATDYPELASVEDVGLSYEGRTMKLLKLGKGGADKPIIFIDGGIHAREWIAPSTVTYIVNEFVS----NSATYDDILSNVNFYVMPTINPDGYAYTFT--DDRLWRKTRSETGSVLGCKGADPNRNWSFHWDEVGASDSPCSDIYAGPEPFSEVEMRNVRDQILEYAANIKVYLTFHSYSQLWMYPWGFTSDLPDDWQDLDTLATNAVDALTAVHGTRYEIGSSTNTIYAAAGGSDDWAKGEGGVKYAYTIELR--DTGNYGFLLPENQIIPTGEETFE 289          
BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Match: sp|O02350|CBPA1_ANOGA (Zinc carboxypeptidase A 1 OS=Anopheles gambiae OX=7165 GN=AGAP009593 PE=2 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 1.050e-59
Identity = 127/399 (31.83%), Postives = 208/399 (52.13%), Query Frame = 2
Query:  137 LFEVTPTNEKQLND------------FIELSQKL--KLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDW-VKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            L+ VTP +E QL              F+E ++KL  + D+     +  P K +  ++     YI   L E N+              +   D E  R+ ++ +A G      Y    +E + WL  L+ ++ K + +++ G+S++ + M  +KLS GP +  +F++GGIHAREW+  ++V +ILN+L++   + +A V+ +  +F+ Y+ P  NPDGY  T +   +RLWRK        C G D NRN+D  +   G S++ C+  Y GS AF   ET +   FV  ++ KL  +++FH+YSQLLL P+ +T     ++ ++  +A     S+ K+ G +Y  G+  + +Y ASG   DW      VK +YT+E+RPD    +GFV P   I P G+E
Sbjct:   39 LYRVTPHSEAQLRSVAAMEQASDSLIFLETARKLGDRFDI-----VVAPHKLADFTETLESDYIPHELIEQNV--------------QRAFDEERVRLTNK-RAKGPFDWNDYHTL-EEIHAWLDQLASEHPKEVELLDAGRSHQNRTMKGVKLSYGPGRPGVFLEGGIHAREWISPATVTYILNQLLT---SEDAKVRALAEKFDWYVFPNANPDGYAYTFQ--VNRLWRKTRKAYGPFCYGADPNRNWDFHWAEQGTSNNACSDTYHGSEAFSEVETRSLAAFVEKLRGKLGAYIAFHSYSQLLLFPYGHTGEHSPNHQDLNEIAEATVKSLAKRYGTQYKYGNVYDAIYPASGSSVDWSYGAQDVKIAYTYELRPDGDAWNGFVLPPNEIVPTGEE 411          
BLAST of carboxypeptidase B-like vs. TrEMBL
Match: A0A1S3I1X9 (carboxypeptidase B-like OS=Lingula unguis OX=7574 GN=LOC106159929 PE=4 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 8.976e-93
Identity = 169/427 (39.58%), Postives = 246/427 (57.61%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFI---ELSQKLKLDLWK---HPS-----IGQPVKFSTESDAEHVTYIRSHLAELN-LDLRIVNENLDDTISKEIKDNEVKR---IHSQFKAA--------------GAAGIETY-----------FAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            L  ++PT + Q+ DF+   E + +L +D W    HP      I  P K        + T+IR  L E+N +  +++ E++   I  E   N   R   I S+   +              G   + T+           +A H E N WL +++ +++   +V  IG++YEGKDM V+K+     K  I++D GIHAREW+  +++V+I++K +S + +     +  LN++  Y+LP+ NPDGYE +     DRLWRK   P    C GVD NRN+D+ FG  G SS+PC+ VYPG+  F    T N RDF+   KD+LK+++SFH+YS+L LTPW + +  PSDNDEMMRVANL   ++   +GK +IVG+P +ILYAASGG YDW K K G+KY+YTFE+RPD    +GFV P   I P+G E+Y
Sbjct:   34 LVRLSPTTQDQI-DFLKRLENTVQLGIDFWTPSDHPGTHNIDIAVPPK-------TYRTFIR--LIEVNKITYKVIQEDIQSLIDTETTSNSKAREDKIKSKACESQGNWKGKDKNKCKNGQVAVATFSNLATDSIVGTYARHGEINAWLDAVAAESNGLATVETIGQTYEGKDMKVLKIGEDGTKPAIWVDAGIHAREWIAPATLVYIIDKFLSEYNS-----RPELNKYTWYMLPVANPDGYEYSHTS--DRLWRKTRSPQSRRCTGVDPNRNWDSHFGEAGTSSNPCSNVYPGTAPFSEKCTQNIRDFINDHKDRLKMYLSFHSYSELWLTPWGWGREKPSDNDEMMRVANLGKTAVQGVNGKSWIVGAPPDILYAASGGSYDWAKEKAGIKYAYTFELRPDGNASNGFVIPASEILPSGKEVY 443          
BLAST of carboxypeptidase B-like vs. TrEMBL
Match: A0A210QZQ6 (Carboxypeptidase A2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT22977 PE=4 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 6.046e-90
Identity = 156/416 (37.50%), Postives = 234/416 (56.25%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQK--LKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAA-----------------GAAGIET------YFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPK--KETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQ--DRSGFVAPVEWISPAGDELY 1294
            L  V PT+ +Q+     L     LKLD W  P + + V       +   +Y++  + +  L  R+  +++  +I  E++D    R+     A                  G+    T       F+ H E ++WL ++  +  +  +V +IG+SYEG+DM +IK+       K  I+++ GIHAREW+  +  VW ++K +  +  ++ DV  +L+ F+ + LP  NPDGYE T     DRLWRK   P   GC GVD NRNFD DFG  G S DPC   YPG++AF  PET N R  + S++D+  +F+SFHAYSQL+LTP+ YT + PSD  EM+R+A+    ++ +  G  + VG+P +ILYAASGG YDW K K GVKYSYT+E+RP     ++SGF+   + I P+G+E++
Sbjct:   31 LLRVHPTSTRQVEFLRNLHDNGNLKLDFWTFPVLDREVDIRVPPRSR--SYVKGLMKKAGLSTRVAMDDIQSSIQNELEDMANARLSKTGPATYNFMCEAFGVNCPPYETGSGSTRTSGEIVGRFSRHSEIDSWLTAIESEFGELATVESIGQSYEGRDMKIIKIGKASPNPKPVIWVEAGIHAREWISPAVAVWTIDKFIRNYGLDD-DVTYMLDTFDWFFLPSANPDGYEYTFT--NDRLWRKTRSPQRRGCYGVDPNRNFDVDFGGAGTSRDPCADTYPGTQAFSEPETDNIRKSIWSVRDRAVVFLSFHAYSQLMLTPYGYTSSKPSDYPEMLRIADASMSALREVHGNTFRVGTPPDILYAASGGTYDWAKLKAGVKYSYTYELRPAEAAWNQSGFIVGEDQIVPSGEEVW 441          
BLAST of carboxypeptidase B-like vs. TrEMBL
Match: A0A1S3I0Q2 (carboxypeptidase B OS=Lingula unguis OX=7574 GN=LOC106159930 PE=4 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 1.173e-87
Identity = 165/429 (38.46%), Postives = 236/429 (55.01%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFI---ELSQKLKLDLWK---HPS-----IGQPVKFSTESDAEHVTYIRSHLAELN-LDLRIVNENLDDTISKEIKDNEVKR--------IHSQ----------------------FKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            L  ++PT + Q+ DF+   E + +L +D W    HP      I  P K        + T+IR  L E N +  +++ E++   I  E   N   R          SQ                      F       I   +A H E N WL +++ +++   +V  IG++YEGKDM V+K+     K  I++D GIHAREW+  +++V+I++K +S + +        LN++  Y+LP+ NPDGYE +     DRLWRK   P   GC GVD NRN+D+ FG  G S+ PC+ +YPG+  F    T N RDF+   KD+LK+++SFH+YS+L LTPW +    PSDN EMMRVANL   ++   +GK + VG+P +ILYAASGG YDW K K G+KY+YT E+RPD    +GFV P   I P+G E+Y
Sbjct:   34 LVRLSPTTQDQI-DFLKRLENTVQLGIDFWTPSDHPGTHNIDIAVPPK-------TYRTFIR--LIEANKITYKVIQEDIQSLIDTETTSNNKAREDKIKSKACESQGNSNWKGKGKNKCKNGQDAVATFSNLATDSIVGTYARHGEINAWLDAVAAESNGLATVETIGQTYEGKDMKVLKIGEDGTKPAIWVDAGIHAREWIAPATLVYIIDKFLSEYNSLPE-----LNKYTWYMLPVANPDGYEYSHT--SDRLWRKTRSPQSWGCRGVDPNRNWDSHFGEAGTSTFPCSDIYPGTGPFSEKCTQNIRDFINEHKDRLKMYLSFHSYSELWLTPWGWGTEKPSDNAEMMRVANLGKTAVQGINGKSWTVGAPPDILYAASGGSYDWAKEKAGIKYAYTLELRPDGNASNGFVIPTSEILPSGKEVY 445          
BLAST of carboxypeptidase B-like vs. TrEMBL
Match: R4G7D1 (Carb-Abd-1 OS=Abdopus aculeatus OX=515833 PE=2 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 1.242e-75
Identity = 143/394 (36.29%), Postives = 225/394 (57.11%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDF--IELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTY--IRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTN---NADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            L +V P  E +L++    E     ++D W  P+    V  S E       +  I   L +  ++L +++EN++  I KE K   V  ++S+        +   +AP ++    +   +D+ S   S+ + GKS+E +D+  IK S GP ++TI ++ G+H+REW+ ++S +    K++  F T    + +V D+L+R++   +P+ NPDGY  T     DR+WRKNT    NGCLG DLNRNFDA +G  G S +PC+  Y G+  F   E+      V S ++K+  F S HAYSQL+LTP+  T+  P D+D +  VA    ++IY+++G+ +IVG+P +ILY A+GG YDW K K+ +KY+Y FE+RPD     GF+ PV  I P+  EL+
Sbjct:   24 LLKVLPHTENELSNLKIFEKDNPEEIDFWISPT---KVNKSAECLVSPAFFHKIEKSLKDAGIELTVLSENIESNIEKE-KPKSV--LYSE------RAVTNTYAPVEKIVELIMGYADKYSH-ASLKHYGKSHENRDLYAIKFSVGPGRKTIIVEAGMHSREWIAIASTL----KVIESFATKRDIDWEVTDLLDRYDWVFVPVANPDGYHMTHT--TDRMWRKNTKRHSNGCLGTDLNRNFDALWGGEGASPNPCHPTYRGTSVFSESESRYLSRLVRSTRNKIAFF-SVHAYSQLILTPYGCTRQKPKDSDHLTYVAEQARNAIYRETGREFIVGTPPDILYPATGGAYDWAKMKMNIKYAYAFELRPDPYSSKGFIMPVSEIQPSYRELF 397          
BLAST of carboxypeptidase B-like vs. TrEMBL
Match: A0A1I8AC41 (Uncharacterized protein OS=Steinernema glaseri OX=37863 PE=4 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 5.111e-75
Identity = 153/406 (37.68%), Postives = 239/406 (58.87%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLK---LDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIE---TYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLST--GPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKD--RLWRKNTMPV------ENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAY-TQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            +F ++PT+E Q+ DF+  +++LK   LD W+ P+ IG P+    + DA H++  R+ L   N+   I+ +N++  I K++KD       + +  A    I      F P+++   + Q+L+      + + +IGK++EG+D+ V+K+ T    KK  I+IDGGIHAREW+  + V++ +++LV  + T+   +K +++  + YI+P+ NPDGYE +R       R WRKN  P          C GVDLNRNFD  FG  G S+DPC  +Y G+  F  PET   RDF+L++ D LK F++FH+Y+Q+L+ P+ + + T P D  E+   A   A+++    G  Y+VG+ A+ILY ASGG  DWVK K+G+KY+Y FE+RP   D SGF+     I P   E +E
Sbjct:   40 VFTLSPTSEDQI-DFLR-NERLKNGMLDFWREPTRIGAPIHVMIK-DA-HISDFRNELNVRNITHEILIKNVEKLI-KKLKDKAATEGANVYSHATKDVINFDFGRFHPYEDVVAYAQALATHFPDRVQIASIGKTHEGRDIPVLKIGTKGNQKKPAIWIDGGIHAREWISPAVVLFFVDQLVGAYSTDPF-IKKLVDSLDWYIVPLLNPDGYEFSRSSTDPVVRFWRKNRSPALCKPDTMECCEGVDLNRNFDWFFGAQGSSTDPCQDIYNGAEPFSEPETAAVRDFLLNVSDDLKSFLTFHSYAQVLMFPYGHLSGTYPGDVTELQSTAIRAANALKSVYGTNYVVGTGADILYPASGGSDDWVKGKLGIKYTYLFELRPQTSDASGFLFDSSQIIPTALETWE 439          
BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Match: ENSAMXT00000011968.2 (carboxypeptidase A1-like [Source:NCBI gene;Acc:111195717])

HSP 1 Score: 230.72 bits (587), Expect = 1.041e-70
Identity = 145/391 (37.08%), Postives = 209/391 (53.45%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQ--KLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPH--KEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  +T  ++ Q+N    L+Q   LKLD W+ P I + +       +  +  ++S L   N+   I+  N+ D + +E +  E+ R  S+    G+     Y   H   E  +++  L  +  K +S V IGKSYEG  +NV+K STG  +  I+ID GIH+REW+  +S  W   K+ + F   NA +  IL +F+I++  +TNPDGY+ T     DR+WRK   P     C+GVD NRN+DA FG  G S DPC+ +Y G  A   PE     DFV S    LK FVS H+YSQ+LL P+ YT+TP  D  E+  V      ++ +     Y  GS  N++Y ASGG  DW    G+KYSYTFE+R  +    GF+ P   I P  +E +
Sbjct:   25 VLRITAEDDLQINLLKNLTQLAYLKLDFWREP-IHESLPVDVSVPSTSLQTLKSFLKNNNILYTIMIHNIQDLLDEEQR--EMNR--SRAFQPGSTDDYDYANYHTLSEIYSFIDLLVKEKPKLVSKVVIGKSYEGHPLNVLKFSTGANRPGIWIDTGIHSREWVTQASGTWFAKKIATDFG-RNAALTAILFKFDIFLEIVTNPDGYDYTHT--TDRMWRKTRKPNSGSDCVGVDPNRNWDAFFGGPGSSRDPCSEIYHGPYAHSEPEVKAIVDFVKS-HGNLKAFVSIHSYSQMLLYPYGYTRTPCKDQAELHEVVRRAVIALQRPYSTNYKYGSIINVVYEASGGSIDWTYNQGIKYSYTFELR--DTGLYGFLLPANQIIPTAEETW 404          
BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Match: CPA1 (carboxypeptidase A1-like [Source:NCBI gene;Acc:103029000])

HSP 1 Score: 218.394 bits (555), Expect = 6.965e-66
Identity = 143/398 (35.93%), Postives = 215/398 (54.02%), Query Frame = 2
Query:  122 ESRMG--LFEVTPTNEKQLNDFIELSQ--KLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPH--KEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            ES +G  +  +T  +E +L    ELSQ   LKLD W  P + + +          +  +R+ LA   +   I+ E+L   +  E ++     I+++     +     Y   H   E N++++ L  +N   +S V IG+SYEG+ +NV+K STG  +  ++ID GIH+REW+  +S  W   K+V+G+ T+ A +  IL++F+I++  +TNPDG++ T     +R+WRK   P   + C+GVD NRN+DA FG +G S+ PC+  Y G  A   PE     DFV S    LK FVS H+YSQ+LL P+ YT+TP  D  E+  +A      +    G  Y  GS    +Y ASGG  DW    G+KYSYTFE+R  +    GF+ P   I P  +E +
Sbjct:   26 ESFVGDQVLRITAKSETELVLLKELSQLEHLKLDFWTEP-VEESLPVDIRVPFHSLQPVRAFLAYHEIHYNIMIEDLQALVDMEQRE----MINTRAPEPRSTDDFDYANYHTLSEINSFIELLVRENPNLVSKVVIGQSYEGRALNVLKFSTGANRPGLWIDTGIHSREWVTQASGTWFAKKIVTGYGTDAA-LTAILDKFDIFLEIVTNPDGFQYTHSS--NRMWRKTRKPNSGSSCVGVDPNRNWDAGFGGSGSSNSPCSETYRGPYAHSEPEVKAIVDFVKS-HGNLKAFVSIHSYSQMLLYPYGYTRTPCKDQAELHELAKKAVTDLKSLYGTSYKYGSIITTIYQASGGTIDWTYDQGIKYSYTFELR--DTGLYGFLLPARQIIPTAEETW 412          
BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Match: cpa2 (carboxypeptidase A1 [Source:NCBI gene;Acc:103026101])

HSP 1 Score: 217.624 bits (553), Expect = 9.830e-66
Identity = 136/392 (34.69%), Postives = 203/392 (51.79%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHP-SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGP-KKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  +   +E+Q+    EL   +  +LD W HP S   PV     S + H    +S L   N+   ++  N+ + + +E +  EV  +  Q ++  +     Y      Y+ W+ +L   ++  +S V+IG SYE + M V+K STG  K+  I+ID GIHAREW+  +S VW+ NK+ + +  +   V  +L R ++Y++ +TNPDGY  T     +R+WRK T  +  G  C GVD NRN+DA FG  G S +PC+  Y G  A    E  N  D V +     K F+S H+YSQLL+ P+ Y+     D  E+  V      ++    G  Y VGS   I+Y ASGG  DW   IG+KYS+ FE+R  +  R GF+ P   I P   E +
Sbjct:   25 VLRINAESEEQIQLLKELEDDEGWELDFWTHPVSTDLPVDIHVPSASLHAA--KSFLHLHNISFTVMINNVQELLDEEQESMEVNAM--QERSVNSFNFGAYHTLDTIYS-WMDTLVAAHANLVSKVHIGFSYENRPMYVLKFSTGGNKRPAIWIDAGIHAREWVSTASAVWMANKMATDYGVD-PSVTSLLGRMDVYLMVVTNPDGYAYTHT--SNRMWRK-TRSINAGSVCRGVDPNRNWDAGFGGPGASKNPCSDSYHGPSAQSEIEVKNVVDLVKN-HGNFKAFISIHSYSQLLMYPYGYSCNNIPDQAELGTVGRSAVQALSSLYGTSYRVGSICRIIYQASGGSIDWSYDIGIKYSFAFELR--DTGRYGFLLPANQIIPTASETW 404          
BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Match: cpa2 (carboxypeptidase A1 [Source:NCBI gene;Acc:103026101])

HSP 1 Score: 217.624 bits (553), Expect = 9.830e-66
Identity = 136/392 (34.69%), Postives = 203/392 (51.79%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELS--QKLKLDLWKHP-SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGP-KKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  +   +E+Q+    EL   +  +LD W HP S   PV     S + H    +S L   N+   ++  N+ + + +E +  EV  +  Q ++  +     Y      Y+ W+ +L   ++  +S V+IG SYE + M V+K STG  K+  I+ID GIHAREW+  +S VW+ NK+ + +  +   V  +L R ++Y++ +TNPDGY  T     +R+WRK T  +  G  C GVD NRN+DA FG  G S +PC+  Y G  A    E  N  D V +     K F+S H+YSQLL+ P+ Y+     D  E+  V      ++    G  Y VGS   I+Y ASGG  DW   IG+KYS+ FE+R  +  R GF+ P   I P   E +
Sbjct:   25 VLRINAESEEQIQLLKELEDDEGWELDFWTHPVSTDLPVDIHVPSASLHAA--KSFLHLHNISFTVMINNVQELLDEEQESMEVNAM--QERSVNSFNFGAYHTLDTIYS-WMDTLVAAHANLVSKVHIGFSYENRPMYVLKFSTGGNKRPAIWIDAGIHAREWVSTASAVWMANKMATDYGVD-PSVTSLLGRMDVYLMVVTNPDGYAYTHT--SNRMWRK-TRSINAGSVCRGVDPNRNWDAGFGGPGASKNPCSDSYHGPSAQSEIEVKNVVDLVKN-HGNFKAFISIHSYSQLLMYPYGYSCNNIPDQAELGTVGRSAVQALSSLYGTSYRVGSICRIIYQASGGSIDWSYDIGIKYSFAFELR--DTGRYGFLLPANQIIPTASETW 404          
BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Match: cpb1 (carboxypeptidase B1 [Source:NCBI gene;Acc:103027518])

HSP 1 Score: 210.69 bits (535), Expect = 4.601e-63
Identity = 123/375 (32.80%), Postives = 197/375 (52.53%), Query Frame = 2
Query:  185 ELSQKLKLDLWKHPSIGQPVKFSTESD----AEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKL--STGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            +L+  +K+D W  P   Q V    + D    A  +  +   L +  +D  ++  +L D ++ ++      + H   K    + I+           W+ S++  N   IS   IG SYEG+ M+++K+  S G  K  IFID GIHAREW+  +   W + + VS + ++ A +  +LN+ ++++LP+ N DGYE T     DR+WRK  +    + C+G D NRNFDA +   G SS PC+  Y GS      E  N  +F+ + K  +K +++ H+YSQLLL P++Y     +D+ E++ VA   + ++    G +Y  G  A  +Y A+GG  DW   +G+KYSYTFE+R  +  R GF+ P   I P  +E
Sbjct:   41 DLASSIKVDFWS-PESEQQVTIDIDVDIHVPAAFLDMVFVKLDQSGMDYEVLIHDLQDALNGQMDSRIQTKSHDYTKYNSWSTIQ----------GWITSITSANPTLISRTTIGNSYEGRPMHLLKIGKSNGSTKPAIFIDCGIHAREWISPAFCQWFVKEAVSTYGSD-AQMTSLLNQMDVFVLPVFNMDGYEHTWS--SDRMWRKTRSKRSGSSCIGADPNRNFDAGWCTLGASSTPCSDTYCGSTPESEIEVKNVANFIRNNKSIIKAYLTIHSYSQLLLFPYSYKYGLAADHAELLSVAEAASTALRSLYGTKYTSGPGATTIYPAAGGSDDWAYDLGIKYSYTFELR--DTGRYGFLLPESQIKPTCEE 399          
BLAST of carboxypeptidase B-like vs. Ensembl Sea Lamprey
Match: cpa5 (carboxypeptidase A5 [Source:ZFIN;Acc:ZDB-GENE-020514-1])

HSP 1 Score: 114.005 bits (284), Expect = 5.571e-28
Identity = 84/255 (32.94%), Postives = 124/255 (48.63%), Query Frame = 2
Query:  539 MNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMA-DSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIG-VKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
             NV K   G ++   +     H R  +   + +WI  KLVS +  ++A +  +LN  + Y   + NPDGY  T +  +DR+WRK       N C+G D NRNF A FG  G SS  C   Y G       E     +FV +   ++K F+  H+YSQ+L+ P+ Y      D+ E++  + + A  S+Y     +Y  G     +Y ASGG  DW  +   V+YSY FE+R  +  R GF+ P   I P   E +
Sbjct:  166 FNVYKYYGGRERPWRWFATKNHHRRRVLRXTGIWIAKKLVSEYGQDSA-ITSLLNTMDWYFEIVANPDGYVYTHE--RDRMWRKTRSQYSGNSCVGTDPNRNFAAGFGGPGSSSSSCTETYRGPSVHSESEVQAIVNFVQA-HPEIKSFLDIHSYSQMLIFPFGYKNELAPDHTELLAKSAVTALTSLYN---TKYTYGPIITTIYQASGGSIDWTYEAAKVRYSYCFELR--DTGRYGFILPANQILPTAQETW 411          
BLAST of carboxypeptidase B-like vs. Ensembl Sea Lamprey
Match: cpz (carboxypeptidase Z [Source:ZFIN;Acc:ZDB-GENE-090313-267])

HSP 1 Score: 69.3218 bits (168), Expect = 7.093e-13
Identity = 65/237 (27.43%), Postives = 95/237 (40.08%), Query Frame = 2
Query:  407 AGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFID-----GGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNF-DADFG---RTGVSSDPCNIVYPGSRAFIAPETINFRD------FVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRV 1072
            AG  G       H E    L+           + +IGKS+E +D+ VI+LS  P K  + +      G IH  E LG   +V +   L +  +  +  V+ +LN   ++ILP  NPDGYE         +         N    +DLNRNF D   G   R    + P  +        +APET    D      FVL+        +  + Y   L+    Y+ TP    D+M R+
Sbjct:  171 AGHRGAVLRHRRHAEMTRALRRAEKSCGGAARLYSIGKSFENRDLLVIELSENPGKHELLVPEVRLVGNIHGNEVLGRELLVALAQHLCARLEDGDERVRRLLNTTRLHILPSMNPDGYELAADSVSGSVANAADSSGRNNMQNIDLNRNFPDLTSGVLRRPKRRTQPLPVPDQYWDGKVAPETRAVIDWMEEFPFVLAGNLHGGELLVRYPYDASLMGEKFYSATP---EDQMFRL 404          
BLAST of carboxypeptidase B-like vs. Ensembl Sea Lamprey
Match: CPXM2 (carboxypeptidase X, M14 family member 2 [Source:HGNC Symbol;Acc:HGNC:26977])

HSP 1 Score: 61.2326 bits (147), Expect = 2.479e-10
Identity = 38/146 (26.03%), Postives = 70/146 (47.95%), Query Frame = 2
Query:  443 HKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETI------FIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKD------RLWRKNTMPVENGCLGVDLNRNF 844
            +KE    ++ ++++      + NIGKS+EG  +  +++S  P +  I      ++  G+H  E LG    ++++  L   +   N  ++ ++    I++LP  NPDGYEK  + G +        W +N         G+DLN NF
Sbjct:  186 YKEMRLLMKIVNEKCPNVTRIYNIGKSHEGLKLYAMEISDNPGEHEIGEPEFRYV-AGVHGNEALGRELTLFLMEFLCHEYNRGNPRIRRLVEETRIHLLPSVNPDGYEKAYEAGSELSGWTFGRWTRN---------GIDLNHNF 321          
BLAST of carboxypeptidase B-like vs. Ensembl Yeast
Match: ECM14 (Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly [Source:SGD;Acc:S000001174])

HSP 1 Score: 137.117 bits (344), Expect = 2.228e-36
Identity = 92/345 (26.67%), Postives = 178/345 (51.59%), Query Frame = 2
Query:  314 DLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNN---WLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLS-----TGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKL--FVSFHAYSQLLLTPWAYT-QTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSP-----ANILYA-ASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            D +++ +N+++ I   +  +++    ++ +A      + +F  +++ +    WL  L       ++V ++G+++EG+++  + +S     + P+K+TI I GGIHAREW+ VS+V W L +L++ + ++  + K  L+  +  ++P+ NPDGY  T     DRLWRKN        CLG+D++ +F   F      +  C+  Y G   F A E   +  ++   K   K+  ++  H+YSQ +L P+AY+    P D + ++ ++  ++ +I  KSG+ Y V S      ++I     +G   D++      +++  ++R  +    GF+ P E I P G E Y
Sbjct:   81 DGQVIIDNMNELIQNTLPTSQMM---AREQAVFENDYDFFFNEYRDLDTIYMWLDLLERSFPSLVAVEHLGRTFEGRELKALHISGNKPESNPEKKTIVITGGIHAREWISVSTVCWALYQLLNRYGSSKKETK-YLDDLDFLVIPVFNPDGYAYT--WSHDRLWRKNRQRTHVPQCLGIDIDHSF--GFQWEKAHTHACSEEYSGETPFEAWEASAWYKYINETKGDYKIYGYIDMHSYSQEILYPYAYSCDALPRDLENLLELSYGLSKAIRSKSGRNYDVISACKDRGSDIFPGLGAGSALDFMYHHRAHWAFQLKLR--DTGNHGFLLPPENIKPVGKETY 415          
BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Match: EDO46863 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNH7])

HSP 1 Score: 196.438 bits (498), Expect = 1.473e-59
Identity = 103/278 (37.05%), Postives = 158/278 (56.83%), Query Frame = 2
Query:  464 LQSLSDQNSKFISVVNIGKSYEGKDMNVIKLST-GPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSD-PCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKI-GVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            +  LS  N   +S+V+ GK+YEG+ +  +K+ T    K   FI+ GIH+REW+ +S+ +++  KLV  +  NN+ V+ ++ +FE  ILP+ N DGY  T    +DR+WRKN  P   GC+G DLNRNF+  +G  G + + PC+ +YPGSR F   ET N   ++   + +L+ +V FH+Y QL ++PW +T+  P +     R  N +  +I +  G  +  G  + ++Y  SG   DW   + GV +SY  E+RP   D  GF  P   I P G E
Sbjct:    1 MTRLSRTNPSTVSLVDFGKTYEGRKIYGLKVGTIYANKPIFFINCGIHSREWISISTCMYVARKLVQTYP-NNSRVQQMVEKFEWIILPVFNVDGYIYTNN--RDRMWRKNKNPGVGGCMGTDLNRNFNYHWGGIGSAPNKPCSPIYPGSRPFSEVETRNVARYLYKKRKRLRAYVDFHSYGQLWMSPWGFTKKFPPNYRRHARAMNAVVKAIKRVHGTEFTYGPSSIMIYPTSGDATDWTFGVLGVTHSYGVELRPGLSDLYGFFLPPSSIIPVGKE 275          
BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Match: EDO45321 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT36])

HSP 1 Score: 185.652 bits (470), Expect = 1.952e-55
Identity = 97/279 (34.77%), Postives = 158/279 (56.63%), Query Frame = 2
Query:  464 LQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQT-PPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKI-GVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + +L  Q+S+  ++ N+G +YEG+ M  +K+S+G  K+T F++ GIHAREW+  ++ +++LN+++  +  +   V  +L++ +  I+PI N DGY  T     +R+WRK   P   GC G D NRN+   +G  G SSDPC   Y G R F   E +N   ++   +  L  ++  HAYSQL +T W + +  PP+ N ++        +++ K  G  Y +G  + I+YA SGG  DW   + G++YSY  E+R  ++ + GFV P   I P G E +
Sbjct:    1 MDNLVRQHSQLATMQNLGNTYEGRPMKAVKISSGRGKKTFFMNCGIHAREWITQATCMYMLNQILYKYGKDQT-VTAMLDKMDFVIMPILNVDGYVYTHT--HNRMWRKTRKPY-GGCYGADPNRNWSFKWGGVGTSSDPCRDTYRGPRPFSEVEALNVARYLYRNRKSLIGYMDIHAYSQLWMTSWGFNRAYPPAYNTKVKPAGQKAVEALRKVYGTHYRLGPSSIIIYATSGGTMDWATGVLGIEYSYGLELR--DEGKYGFVLPANQILPTGVETF 273          
BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Match: EDO46954 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNM7])

HSP 1 Score: 186.422 bits (472), Expect = 1.577e-54
Identity = 125/403 (31.02%), Postives = 207/403 (51.36%), Query Frame = 2
Query:  131 MGLFEVTPTNEKQLNDFIEL--SQKLKLDLWKHPSIGQ-PVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAA-GIETYFAPHKEYNNWLQSLSDQNSKF-----ISVVNIGKSYEGKDMNVIKLS--TGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG-CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKR--YIVGSPANILYAASGGCYDWV-KKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            M +  V P +E +++   EL   + +K+D W  PS  + PV         HVT   +H  +     R+      + I   ++  +V+R+  Q K A A     ++++ +   +     L +  + +     + V  +GKSY GKD   I++       K   F++ GIHAREW+  ++ ++I  +L+S +   +A+V  +L++ +  I+P+ NPDGY  T +  + R WRK   P     C+G D NRN+D  +G  G S+DPC   Y G  AF   E +N   ++  + D++K ++  HAYSQ  + PW +TQTP  D  E+MRV+   A++I + SG    Y VG  + ++Y  SGG  D+    +G+KYS+  E+R  +  + GF+ P E I P   E +
Sbjct:    1 MKVVRVIPQDEAEIHVINELRNHKDIKVDFWAPPSRPRHPVDL-------HVT---AHAYK-----RLARHLSANRIPFTLQIPDVQRLVDQQKMATAGRASASWYSTYHTLDQIYAELKNLYNTYYRNGNVRVFTVGKSYNGKDQKAIEIKGKRARNKPVFFMNCGIHAREWVSPATCMYIAKQLLSRY-GKDANVTSVLDKMDFVIMPVLNPDGYVFTWE--RQRFWRKTLKPNSGTFCVGTDPNRNWDYKWGAEGASTDPCADDYRGPHAFSEIEIVNVAKYLKKLGDRIKGYMDIHAYSQFWMIPWGHTQTPSKDYQELMRVSKAGAEAI-RSSGDNTVYKVGPSSTVIYVNSGGSKDYTYGALGIKYSFALELR--DTGKEGFLLPPEQIIPTALETF 382          
BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Match: EDO46924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNH8])

HSP 1 Score: 168.318 bits (425), Expect = 6.106e-48
Identity = 115/391 (29.41%), Postives = 182/391 (46.55%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIE--LSQKLKLDLWKHP-SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIK-LSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKI-GVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            L+ V P ++ Q+ DF++  L    +LD W  P +  + V      D          L   +++ +IV ++    +++E              +A + G ++ +    E +  + +L+   S   S  ++GKSYE +D   IK L TG                                    +++DV D+L+ +E  ILP+ N DGYE TR   KDR+WRK   P    C+GVD NRNF+  FG TG S++ C+ +Y G +AF    T N   F+   + +LK ++ FHAYSQ+ L+PW YT   PS+  E M+      +++    G RY+ G  +  +Y  +G   DW   + G  +SY  E+RP   +  GF+ P   I P G E +
Sbjct:   27 LYRVVPRDQAQV-DFLDDLLKTDGELDFWAFPVAPMKNVDIHVSPDKRQA--FEELLEASDMEFKIVVQDFSSMLAQEKV------------SARSGGFDSDYHSLSEIHAEILALAKAYSSVASNFSLGKSYENRDQLAIKILETG-----------------------------------DSDSDVTDMLDNYEWVILPVLNVDGYEYTRT--KDRMWRKTRTP-GTKCVGVDPNRNFNYRFGVTGTSNNECSQIYRGKQAFSEKVTYNVAKFLYENRHRLKGYIDFHAYSQMFLSPWGYTDAKPSNYTEHMKAMTAAVNALQAVHGTRYVFGPASTTIYPTAGDTTDWTYGVLGCVHSYCVELRPTG-NPPGFILPPAQIIPTGQETF 363          
BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Match: EDO43305 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYY0])

HSP 1 Score: 162.155 bits (409), Expect = 2.667e-45
Identity = 108/401 (26.93%), Postives = 193/401 (48.13%), Query Frame = 2
Query:  128 RMGLFEVTPTNEKQLNDFIELSQKLKLDLWKH---PSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSD-QNSKFISVVNIGKSYEGKDMNV-IKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMP------VENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWV-KKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            R  +  V P  + ++    +L     +  WK    P +   +       A +   +  HL +  L   I   +L   +     DNE      +     + G  + + P  +  + ++ L+  Q    +++  +G +YEG ++   IK  + P+K   F++ GIHAREW+  ++ ++I+ + ++ ++++ + V ++L++ +  I+P+ N DGY  T  G  +R+WRK   P       +  C G D NRN+   +G  G SS+PC+  + G  A    E  N   F+  +  ++K ++  HAYSQ  + PW YT     D+ E+MRV+ +  D+I +  G  Y  GS +NI+Y  SG   D+    + +KYS+  E+R  +  R GF+ P   I P   E +
Sbjct:    4 RDKVIRVIPKTDDEVRILADLEHDPTVSFWKEAHAPGMAADIHVP----ARYYGDVARHLDKNGLKFAIQIIDLQSEV-----DNE------KLTLGRSEGFFSGYKPLDKIEDEMRRLAGVQTLAQVTMNTVGSTYEGPNLVFQIKGRSLPEKPVFFMNCGIHAREWITPATCMYIITQFITKYRSDKS-VTEVLDKMDFIIMPVLNADGYVYTWTG--NRMWRKTRKPNIQSWWGQKTCYGTDPNRNWGYKWGLPGASSNPCSDTFRGKTAQSEVEVDNVAKFLKGLGPRVKGYMDIHAYSQFWMIPWGYTTANAKDHKELMRVSKIGVDAIRQTYGTAYRYGSSSNIIYKNSGSSKDYTYGHLNIKYSFALELR--DTGRYGFLLPPGQILPTALETF 384          
BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Match: ENSORLT00000024618.2 (carboxypeptidase A1 [Source:NCBI gene;Acc:101163721])

HSP 1 Score: 217.624 bits (553), Expect = 1.090e-65
Identity = 128/391 (32.74%), Postives = 210/391 (53.71%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLK--LDLWKHPS-IGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +  + P +E Q +    L + ++  LD W+  + +  PV     S A  + +++  L   N++  ++ ++L   + +E ++ +        +   +   E Y   ++ Y+ +   L  +N   +S + IG SYEG+ +NV+K STG   +  I+ID GIH+REW+  +S  W   K+V  + ++ A + DILN  +I++  +TNPDGY+ T+    +R+WRK   P   + C+GVD NRN+D+ FG  G SS PC+  Y G  A    E     DFV++ K+ +K F++ H+YSQ+LL P+ YT  P  D  E+  +A      +    G RY  GS  + +Y ASG   DW  + G+KYSYTFE+R  +    GF+ P + I P  +E +
Sbjct:   25 VLRIVPKDEVQRSQLKNLEEMMESELDFWREVTRVSTPVDVRVPSHA--LQFVKDFLGNQNIEYSVMIQDLQALLDEEQEEMDAAAFSGSPRNTDSFDYENYHTLNELYS-FQDMLVAENPNLVSKLVIGNSYEGRPLNVLKFSTGGTNRPAIWIDTGIHSREWVTQASGTWFAKKIVKDYGSDPA-LTDILNNMDIFLETVTNPDGYDYTQN--SNRMWRKTRKPNPGSSCVGVDPNRNWDSGFGEPGASSSPCSETYRGPSAHSESEVKAIVDFVVAHKN-IKAFITIHSYSQMLLYPFGYTSAPSKDEAELHSLAQKAITDLASLYGTRYTYGSIIDTIYQASGNTVDWTYEQGIKYSYTFELR--DTGFYGFLLPAKQIVPTAEETW 406          
BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Match: CPA1 (carboxypeptidase A1 [Source:NCBI gene;Acc:101163471])

HSP 1 Score: 214.927 bits (546), Expect = 1.414e-64
Identity = 137/393 (34.86%), Postives = 209/393 (53.18%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLK--LDLWKH-PSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHK--EYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMP-VENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            + ++ P N+ QL    +L   L+  LD WK   ++  PV     +  + +  ++  L   ++    + ++L   + +E K+ E+     + K   +     Y   HK  E  ++   L  +N   +S + IG+SYE + ++V+K STG   +  I+ID GIHAREW+  +S +W   K+V+ +   +  + DILN  +I +  +TNPDGYE T     +RLWRK+  P   + C+GVDLNRN+D+ FG  G SS+PC+  Y G RA    E  +  DFV      +K F+S HAYSQLLL P+ +T+ P  D  E+  +A      +    G RY  GS  + +Y ASGG  DW    G+KYSYTFE+R  +  R GF+ P   I P  +E +
Sbjct:   25 VLQIIPKNDAQLAFLKDLEDVLEFELDFWKGVTAVSCPVH--VRAPFQFLQLLKRMLKRKDISYVTMIDDLQAVLDEEQKEIELFARSLKPKNTDSFNYSRY---HKLSEIYSFQDMLVAENPNLVSKIVIGQSYEARPLSVLKFSTGGTNRRAIWIDTGIHAREWITQASGIWFAKKIVTDYG-RDPILTDILNGMDILLEIVTNPDGYEYTH--STNRLWRKSRKPNPRSNCIGVDLNRNWDSGFGGPGASSNPCSETYHGPRAHSESEVKSIVDFV-KTHGNIKAFISIHAYSQLLLYPYGHTRNPAKDQTELDSLAQKAVKDLASLYGTRYRYGSIISTIYQASGGSIDWAYDQGIKYSYTFELR--DTGRHGFLLPSSQIIPTSEETW 406          
BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Match: cpb1 (carboxypeptidase B1 [Source:NCBI gene;Acc:101169819])

HSP 1 Score: 204.527 bits (519), Expect = 1.131e-60
Identity = 120/393 (30.53%), Postives = 211/393 (53.69%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESD----AEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEV--KRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKL--STGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDE 1288
            +  + P   + +    +L++ +++D W  P   + V    + D    A +++ + + L + ++   I+ E++   I+++  DN +  K +HS  K          +       +W+ S++  N   +S   IG ++EG+ M ++KL  S+G  K +IF+D GIHAREW+  +   W + + +  + ++ + +  +L++ ++Y+LP+ N DGYE +     +R+WRK    +  + CLG D NRNFDA +   G SS+PC+  + GS      E  N  DF+   K  +K +++ H+YSQLLL P++Y+    +D+ E+M VA   + ++    G RY  G  A+ +Y A+GG  DW   +GVKYSYTFE+R  +    GF+ P   I P  +E
Sbjct:   25 VLRLKPVLAEHVTLIRDLAESIEVDFWS-PDSPELVTIDIDVDIRVPARYLSIVYTTLGQSSMAHEILIEDVQVAINRQ-ADNGISPKELHSYTK----------YNTWDTVLSWINSIASSNPSLVSKQVIGNTFEGRPMTLLKLGKSSGTTKPSIFLDCGIHAREWISPAFCQWFVKEALETYGSD-SQMTSLLDQMDVYVLPVFNIDGYEYSHT--TNRMWRKTRSKLSGSSCLGTDPNRNFDAGWCTVGASSNPCSETFCGSTPESEIEVKNVADFIRKNKSSIKAYLTIHSYSQLLLFPYSYSYNLAADHSELMSVAQGASAALTSLYGTRYTSGPGASTIYLAAGGSDDWAYDLGVKYSYTFELR--DTGYHGFLLPESQIKPTCEE 400          
BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Match: cpa4 (carboxypeptidase A2 [Source:NCBI gene;Acc:101159188])

HSP 1 Score: 199.134 bits (505), Expect = 5.364e-59
Identity = 128/379 (33.77%), Postives = 192/379 (50.66%), Query Frame = 2
Query:  182 IELSQKLKLDLWKHP-SIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDD----TISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG-PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENG--CLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELY 1294
            +E  ++ +LD W HP S   PV     S +  ++ ++      N+   I+ +NL         +  ++ + +R            +ET ++       W+ +L  Q    ++   IGKSYE + M V+K STG   +  I+ID GIH+REW+  ++ +W  NK+ S F ++ + +  +LN  +IY+L + NPDGY  T     DR+WRK T  + +G  C GVD NRN+DA FG  G S+ PC+  Y G  A    E  N  D + S     K F+S HAYSQLL+ P+ YT    +D  E+  +    A  +    G  Y VG+  NI+Y ASGG  DW    G+KYS+ FE+R  +    GF+ P   I P   E +
Sbjct:    9 LEHEEEWQLDFWLHPVSTDLPVDIRVPSSS--LSSVKDFFLTHNIPFTIIIQNLQGLIEMEKIEMEENQKKERSTKNINFGAYHTLETIYS-------WMDTLVAQYPNLVTKEEIGKSYENRPMYVLKFSTGGANRPAIWIDTGIHSREWVSPATGLWTANKIASDFGSDTS-LTSLLNTMDIYMLILANPDGYVYTHT--NDRMWRK-TRSINSGSSCRGVDPNRNWDAGFGGPGASTSPCSDSYHGPFAHSEIEVKNVVDLIKS-HGNFKSFISVHAYSQLLMYPYGYTCADVADKAELDSIGREAAQKLTSLYGTYYKVGNICNIIYPASGGSIDWSYDSGIKYSFAFELR--DTGYYGFLLPANQIIPTASETW 371          
BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Match: cpb2 (carboxypeptidase B2 [Source:NCBI gene;Acc:101175299])

HSP 1 Score: 190.274 bits (482), Expect = 1.634e-55
Identity = 119/387 (30.75%), Postives = 199/387 (51.42%), Query Frame = 2
Query:  146 VTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAE-LNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTG--PKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVEN-GCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRS--GFVAPVEWISPAGDE 1288
            +TP  ++Q++    ++ + +  LW+  S   PV    +++        S   E +    R+   N+++ I  +  +    R  S F        E Y      Y+ WL   +  N + +  + +G S+E + +  +KLS    P ++ ++ID GIHAREW+  +  +W ++ +     T   ++ DILN  ++Y+LP+ NPDGY+ T     +R+WRKN  P  N  C+GVDLNRNFDA++   G    PC+ +Y G+     PE+    DF+   KD ++L++S H+YSQ+LL P++ T     ++++++ +    A  I       Y  G+ A  +Y A GG  DW   +G+KYS+TFE+    QDR   GF+ P   I  A +E
Sbjct:   12 ITPETQEQVDILKNVTSQYETALWQPVS---PVYMKEQAEVHLFVPGESFETEQVYSGFRVSLPNVNELIEMQTMNGTDPRSTSTF-------YERYHRLEDIYD-WLNRTAHDNPRTVKTILVGSSHEKRPLYALKLSFSDRPNRKAMWIDCGIHAREWISPAFCLWFVHHV----STVKVEITDILNNMDVYVLPVMNPDGYKYT--WTTNRMWRKNRAPSHNPKCVGVDLNRNFDANWCTEGALPYPCSEIYCGAFPESEPESRAVADFLREHKDSIQLYISIHSYSQMLLFPYSCTVEEAGNHNDLLEMVQEAARKIRTFYRSTYKYGAGAKTIYLAPGGSDDWAYDLGIKYSFTFEL----QDRGEYGFLLPPSRIGNACNE 377          
BLAST of carboxypeptidase B-like vs. Planmine SMEST
Match: SMESG000063633.1 (SMESG000063633.1)

HSP 1 Score: 815.453 bits (2105), Expect = 0.000e+0
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 2
Query:  122 ESRMGLFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            ESRMGLFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE
Sbjct:   17 ESRMGLFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 408          
BLAST of carboxypeptidase B-like vs. Planmine SMEST
Match: SMESG000066311.1 (SMESG000066311.1)

HSP 1 Score: 283.108 bits (723), Expect = 6.346e-91
Identity = 150/396 (37.88%), Postives = 237/396 (59.85%), Query Frame = 2
Query:  137 LFEVTPTNEKQLNDFIELSQKLKLDLWKHPSIGQPVKFSTES---DAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE---NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDN---QDRSGFVAPVEWISPAGDELY 1294
            + E++   ++++   +++S+K + D+WK PS     K +T       E+       L +  +  +I+  N+   I +E   NE  +    F A     I  +FA ++E  +W+  L+ + S F+S  + GKSYEG+D+  +K+STG  ++ + ++GGIH+REWL  S+V +I+ K++S   TNN+ +  +L++F++Y +P+ NPDG   +   G +RLWRKN  P       C GVDLNRNFDA FG  G SS+PC+ +Y G  AF  PET +F  FV  +   L LF S HAY+Q    P+ +  + P D+ E+ RVAN+   ++Y+ + +R+ +GSP  +LYAA+GG +DWVK K  VKY+YT E+RP     +  SGF+AP+  IS +G+E+Y
Sbjct:   26 VIEMSLKTDEEIKHVLQISEKYQADIWKGPS-----KITTTHVMLSPEYFDGFIESLTKYEIQYKIMINNVQKIIDEETGMNENVKFMKSFSATRPT-INDHFADYEEIMDWINYLTVKYS-FVSSGSYGKSYEGRDLRYVKISTGQNRKAVLVEGGIHSREWLATSTVTYIIEKMISNILTNNSRIVSLLSKFDVYFIPVFNPDGNVYSHLHG-NRLWRKNRRPFPLDGRTCFGVDLNRNFDAQFGVAGTSSNPCDDLYRGPNAFSEPETKHFSIFVKKLGLNLSLFFSLHAYAQFWFVPYGFGFSRPVDDLELRRVANIGNQALYEVNHERFYIGSPPELLYAAAGGSFDWVKMKARVKYAYTLELRPSRYNVRGSSGFIAPISDISKSGEEVY 413          
BLAST of carboxypeptidase B-like vs. Planmine SMEST
Match: SMESG000043661.1 (SMESG000043661.1)

HSP 1 Score: 211.46 bits (537), Expect = 1.163e-63
Identity = 103/286 (36.01%), Postives = 164/286 (57.34%), Query Frame = 2
Query:  449 EYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNAD--VKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKN-TMPVENGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            E+ NW++ L    +  + +  +GKS+E +++  IKL    +   + +D G HAREWL ++S+V  +  +++       D   K IL ++ IY +P+ NPDGY  + K  ++R WRKN  + V++ C GVD+NRN+   F     ++DPC   +PG   F   E+ + R  V SI+  LKL+VS H Y Q +L PWAY     SD+  ++++AN +  ++++   K + VG+P  +LY A G  +DWVK +GV YSYT E+ P      GF+    +I  + DE+YE
Sbjct:  113 EFENWIRMLKSTRNSLVKISVLGKSFESRNIYSIKLGENIQAPNVLLDSGSHAREWLSIASLVGTIKIILNDLNQPGEDGLWKGILRKWNIYFVPVLNPDGYFHSMKSLENRFWRKNRQLNVDSNCTGVDINRNYPIGFTE---NNDPCQEDFPGGAPFTTLESQSLRKIVSSIQSNLKLYVSLHCYGQFILLPWAYKNEKVSDHTNLVKLANDIQKTMFRVGNKNFKVGTPGELLYTAGGTSFDWVKSLGVPYSYTIELFPSEDKTLGFIVSENFIKNSTDEVYE 395          
BLAST of carboxypeptidase B-like vs. Planmine SMEST
Match: SMESG000056472.1 (SMESG000056472.1)

HSP 1 Score: 190.66 bits (483), Expect = 1.626e-55
Identity = 110/341 (32.26%), Postives = 189/341 (55.43%), Query Frame = 2
Query:  284 IRSHLAELNLDLRIVNENLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYTQTP-PSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVK-KIGVKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            +++ L   N+   I+  +LD  I  E   N  +   ++F+      I +Y+    E   WL   + Q S F+ +  +G S E +++ ++K+S    K  ++ID GIHAREW+  S+ ++++NKL+     N+ +   +L  ++IYILP+ NPDGYE T +   +R+WRKN +      C GVDLNRN+   +G  G S DPC+ ++ GS      ET    + +  IK  +KLF++ H++ Q +++P+ Y++   P    ++M +   ++  + +  G+ Y VGS AN+LY ASG   D+   ++ + YSYT E+ PD + R GF+ P  +I   G++ + 
Sbjct:   84 VKAFLKVENVPFEIMKNDLDRKIKFEQLINRRQYFKTKFQRTDQGSI-SYYMRFVEIKQWLNEKARQVS-FMKLETLGHSEENREIILVKMSKDDSKPILWIDAGIHAREWISPSTAIFLINKLLE----NDKNSMKLLEDYQIYILPLLNPDGYEYTHE--VNRMWRKNRVSTSYEDCKGVDLNRNYPYHWGENGASPDPCSRIFQGSSPSSEKETKAVINKLTEIKTNVKLFITLHSFGQYIMSPYGYSKNRFPRQYYKLMSMGTSVSSFLQEMYGRTYQVGSSANLLYEASGASDDYAAGELRIPYSYTIEL-PD-RGRYGFLLPPTYIQSVGNQFFH 414          
BLAST of carboxypeptidase B-like vs. Planmine SMEST
Match: SMESG000048042.1 (SMESG000048042.1)

HSP 1 Score: 174.866 bits (442), Expect = 1.511e-49
Identity = 103/324 (31.79%), Postives = 177/324 (54.63%), Query Frame = 2
Query:  335 NLDDTISKEIKDNEVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKSYEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKTNNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVE-NGCLGVDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKLKLFVSFHAYSQLLLTPWAYT-QTPPSDNDEMMRVANLMADSIYKKSGKRYIVGSPANILYAASGGCYDWVKKIG-VKYSYTFEVRPDNQDRSGFVAPVEWISPAGDELYE 1297
            N  + I +E  +N      S F+  G++     +  +++   W++    Q   +   V  G++YE + + ++KLS       +++DGGIHAREW+  SS ++I++KL+     N+++  +IL++++ Y++P+ NPDGYE + +    RLWRKN      + C+GVD+NRNF  ++G    S +PC+  Y G  A    E  +    +   KD++KL VS HA+ Q +L P+ ++ +  P +  +M  VA   A  +  K    Y+VG P +ILY ASG   D+V  +G +K  ++F V   +Q   GF  P ++I   G +L++
Sbjct:  101 NYQNLIDREFAENRQAIFQSLFRT-GSSSDHIAYLRYQQIEEWIKE-KVQGVNYAKFVKFGETYENRKLFMVKLSKNDSLPIVWLDGGIHAREWIATSSALFIIDKLLR----NSSETWEILSKYQFYVVPLVNPDGYEYSHE--THRLWRKNRSRFTFSKCIGVDMNRNFPFEWGGASSSPNPCDDQYRGPNAGSEKEIQSIISELTKYKDRIKLVVSLHAFGQYILQPFGFSNEHYPLNYLDMTSVAISAAFDLQTKHDALYLVGRPPDILYPASGSSDDFV--VGTLKIPFSFTVELPDQGMHGFTLPPKYIKSVGSQLWD 414          
The following BLAST results are available for this feature:
BLAST of carboxypeptidase B-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CPA28.807e-7235.10carboxypeptidase A2 [Source:HGNC Symbol;Acc:HGNC:2... [more]
CPO3.680e-6036.27carboxypeptidase O [Source:HGNC Symbol;Acc:HGNC:21... [more]
CPA56.537e-5932.07carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:1... [more]
CPA56.537e-5932.07carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:1... [more]
CPA56.537e-5932.07carboxypeptidase A5 [Source:HGNC Symbol;Acc:HGNC:1... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Y18H1A.94.031e-6533.33pep chromosome:WBcel235:I:748228:757010:-1 gene:WB... [more]
Y47G6A.191.069e-5930.10pep chromosome:WBcel235:I:3523334:3529956:-1 gene:... [more]
Y47G6A.192.192e-5930.10pep chromosome:WBcel235:I:3523313:3529956:-1 gene:... [more]
T06A4.12.276e-5933.82pep chromosome:WBcel235:I:770892:776369:1 gene:WBG... [more]
ZC434.92.207e-5630.71pep chromosome:WBcel235:I:10356251:10360887:1 gene... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG85602.803e-6136.96gene:FBgn0035781 transcript:FBtr0332725[more]
CG85602.803e-6136.96gene:FBgn0035781 transcript:FBtr0076868[more]
CG85627.603e-6032.23gene:FBgn0035779 transcript:FBtr0076854[more]
CG184173.532e-5933.02gene:FBgn0035780 transcript:FBtr0076855[more]
CG30971.607e-5732.12gene:FBgn0029804 transcript:FBtr0070836[more]
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BLAST of carboxypeptidase B-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cpa51.881e-6736.20carboxypeptidase A5 [Source:ZFIN;Acc:ZDB-GENE-0205... [more]
cpb12.774e-6732.23carboxypeptidase B1 (tissue) [Source:ZFIN;Acc:ZDB-... [more]
cpb12.774e-6732.23carboxypeptidase B1 (tissue) [Source:ZFIN;Acc:ZDB-... [more]
cpa23.193e-6334.04carboxypeptidase A2 (pancreatic) [Source:ZFIN;Acc:... [more]
cpa15.334e-6232.05carboxypeptidase A1 (pancreatic) [Source:ZFIN;Acc:... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kpna12.397e-7034.62karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-10... [more]
kpna17.955e-6934.79karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-10... [more]
kpna12.872e-6538.32karyopherin alpha 1 [Source:Xenbase;Acc:XB-GENE-10... [more]
znfx11.218e-5933.85zinc finger NFX1-type containing 1 [Source:Xenbase... [more]
cpb25.046e-5830.67carboxypeptidase B2 [Source:NCBI gene;Acc:595005][more]
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BLAST of carboxypeptidase B-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cpa23.540e-7135.01carboxypeptidase A2, pancreatic [Source:MGI Symbol... [more]
Cpa52.359e-6031.39carboxypeptidase A5 [Source:MGI Symbol;Acc:MGI:192... [more]
Cpa52.359e-6031.39carboxypeptidase A5 [Source:MGI Symbol;Acc:MGI:192... [more]
Cpa43.884e-5932.66carboxypeptidase A4 [Source:MGI Symbol;Acc:MGI:191... [more]
Cpa33.080e-5630.50carboxypeptidase A3, mast cell [Source:MGI Symbol;... [more]
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BLAST of carboxypeptidase B-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P19222|CBPA2_RAT1.164e-7234.87Carboxypeptidase A2 OS=Rattus norvegicus OX=10116 ... [more]
sp|P48052|CBPA2_HUMAN4.230e-7135.10Carboxypeptidase A2 OS=Homo sapiens OX=9606 GN=CPA... [more]
sp|Q504N0|CBPA2_MOUSE2.476e-7035.01Carboxypeptidase A2 OS=Mus musculus OX=10090 GN=Cp... [more]
sp|P04069|CBPB_ASTAS2.488e-6540.48Carboxypeptidase B OS=Astacus astacus OX=6715 PE=1... [more]
sp|O02350|CBPA1_ANOGA1.050e-5931.83Zinc carboxypeptidase A 1 OS=Anopheles gambiae OX=... [more]
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BLAST of carboxypeptidase B-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3I1X98.976e-9339.58carboxypeptidase B-like OS=Lingula unguis OX=7574 ... [more]
A0A210QZQ66.046e-9037.50Carboxypeptidase A2 OS=Mizuhopecten yessoensis OX=... [more]
A0A1S3I0Q21.173e-8738.46carboxypeptidase B OS=Lingula unguis OX=7574 GN=LO... [more]
R4G7D11.242e-7536.29Carb-Abd-1 OS=Abdopus aculeatus OX=515833 PE=2 SV=... [more]
A0A1I8AC415.111e-7537.68Uncharacterized protein OS=Steinernema glaseri OX=... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000011968.21.041e-7037.08carboxypeptidase A1-like [Source:NCBI gene;Acc:111... [more]
CPA16.965e-6635.93carboxypeptidase A1-like [Source:NCBI gene;Acc:103... [more]
cpa29.830e-6634.69carboxypeptidase A1 [Source:NCBI gene;Acc:10302610... [more]
cpa29.830e-6634.69carboxypeptidase A1 [Source:NCBI gene;Acc:10302610... [more]
cpb14.601e-6332.80carboxypeptidase B1 [Source:NCBI gene;Acc:10302751... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cpa55.571e-2832.94carboxypeptidase A5 [Source:ZFIN;Acc:ZDB-GENE-0205... [more]
cpz7.093e-1327.43carboxypeptidase Z [Source:ZFIN;Acc:ZDB-GENE-09031... [more]
CPXM22.479e-1026.03carboxypeptidase X, M14 family member 2 [Source:HG... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
ECM142.228e-3626.67Putative metalloprotease with similarity to zinc c... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO468631.473e-5937.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO453211.952e-5534.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO469541.577e-5431.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO469246.106e-4829.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO433052.667e-4526.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of carboxypeptidase B-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000024618.21.090e-6532.74carboxypeptidase A1 [Source:NCBI gene;Acc:10116372... [more]
CPA11.414e-6434.86carboxypeptidase A1 [Source:NCBI gene;Acc:10116347... [more]
cpb11.131e-6030.53carboxypeptidase B1 [Source:NCBI gene;Acc:10116981... [more]
cpa45.364e-5933.77carboxypeptidase A2 [Source:NCBI gene;Acc:10115918... [more]
cpb21.634e-5530.75carboxypeptidase B2 [Source:NCBI gene;Acc:10117529... [more]
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BLAST of carboxypeptidase B-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063633.10.000e+0100.00SMESG000063633.1[more]
SMESG000066311.16.346e-9137.88SMESG000066311.1[more]
SMESG000043661.11.163e-6336.01SMESG000043661.1[more]
SMESG000056472.11.626e-5532.26SMESG000056472.1[more]
SMESG000048042.11.511e-4931.79SMESG000048042.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026378 ID=SMED30026378|Name=carboxypeptidase B-like|organism=Schmidtea mediterranea sexual|type=transcript|length=1378bp
ATTTGTCGTATTTCTGTGCTCAGTAATCAAGAAATTACTTAAAAGTATTC
TCAATTGCTTGTGATTCATAATTATGATCTTTGGGATAATAATCACTATT
TTGGCCCTCGCGCAACTCTTGGAGTCCAGAATGGGACTTTTTGAGGTGAC
GCCAACAAATGAAAAACAATTGAATGATTTCATAGAACTATCACAGAAAT
TAAAGTTAGATCTGTGGAAACATCCTTCGATTGGGCAACCTGTGAAGTTT
TCTACCGAGAGCGATGCAGAGCATGTCACATATATCCGAAGTCACCTGGC
GGAACTTAATCTGGATCTCAGAATTGTTAATGAAAACTTAGATGACACTA
TTTCCAAGGAAATAAAAGACAATGAAGTAAAAAGAATTCATTCGCAGTTC
AAAGCAGCCGGTGCTGCTGGCATTGAGACATATTTTGCTCCCCACAAAGA
ATATAACAATTGGTTGCAATCTTTAAGCGATCAAAACTCTAAATTCATAT
CTGTGGTCAACATTGGCAAGTCTTATGAAGGAAAGGACATGAATGTTATA
AAACTAAGTACTGGACCAAAGAAAGAAACCATATTTATTGATGGAGGAAT
TCATGCTAGAGAATGGCTTGGCGTTTCGTCGGTTGTTTGGATATTGAATA
AGTTGGTTTCTGGATTCAAGACAAATAATGCAGATGTTAAGGATATTTTA
AACCGATTTGAAATCTATATTCTACCTATCACTAATCCAGATGGCTACGA
GAAGACAAGGAAGGGAGGAAAAGACAGATTATGGAGAAAAAATACAATGC
CTGTAGAAAATGGCTGCCTTGGAGTGGATCTAAACCGAAACTTTGATGCA
GATTTTGGAAGAACTGGTGTTAGTTCGGATCCTTGTAACATCGTTTATCC
AGGATCTCGGGCTTTCATTGCTCCAGAAACTATAAATTTCAGAGATTTTG
TTCTTTCAATAAAAGATAAGCTTAAACTGTTTGTTTCGTTCCATGCTTAT
TCACAATTACTTCTCACTCCTTGGGCTTACACTCAGACTCCTCCGTCAGA
CAATGATGAGATGATGAGAGTTGCCAATTTGATGGCCGATTCTATCTATA
AAAAATCTGGAAAGCGATATATTGTTGGGTCACCGGCAAATATTTTATAT
GCTGCCTCTGGTGGTTGCTACGACTGGGTAAAGAAGATTGGAGTTAAGTA
TTCATACACTTTTGAGGTACGACCTGACAATCAGGATAGAAGCGGATTTG
TGGCACCAGTAGAATGGATCAGCCCGGCTGGAGATGAATTATATGAAGCC
ATAATAGTTGCGGCAAAATCAGTCAAATAGATTGGAAAATGAAAAAAACT
TAATAGAAATAAATGAATGTTTTTTTTG
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protein sequence of SMED30026378-orf-1

>SMED30026378-orf-1 ID=SMED30026378-orf-1|Name=SMED30026378-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=419bp
MIFGIIITILALAQLLESRMGLFEVTPTNEKQLNDFIELSQKLKLDLWKH
PSIGQPVKFSTESDAEHVTYIRSHLAELNLDLRIVNENLDDTISKEIKDN
EVKRIHSQFKAAGAAGIETYFAPHKEYNNWLQSLSDQNSKFISVVNIGKS
YEGKDMNVIKLSTGPKKETIFIDGGIHAREWLGVSSVVWILNKLVSGFKT
NNADVKDILNRFEIYILPITNPDGYEKTRKGGKDRLWRKNTMPVENGCLG
VDLNRNFDADFGRTGVSSDPCNIVYPGSRAFIAPETINFRDFVLSIKDKL
KLFVSFHAYSQLLLTPWAYTQTPPSDNDEMMRVANLMADSIYKKSGKRYI
VGSPANILYAASGGCYDWVKKIGVKYSYTFEVRPDNQDRSGFVAPVEWIS
PAGDELYEAIIVAAKSVK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036990M14A-like_propep
IPR000834Peptidase_M14
IPR003146M14A_act_pep
IPR009020Propept_inh
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004181metallocarboxypeptidase activity
GO:0008270zinc ion binding
GO:0004180carboxypeptidase activity
GO:0005515protein binding
GO:0008233peptidase activity
GO:0008237metallopeptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
GO:0005615extracellular space
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 75..95
NoneNo IPR availableGENE3DG3DSA:3.40.630.10coord: 118..418
e-value: 3.9E-96
score: 323.9
NoneNo IPR availablePANTHERPTHR11705PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,Bcoord: 14..414
NoneNo IPR availableCDDcd03860M14_CP_A-B_likecoord: 123..417
e-value: 1.75425E-114
score: 334.881
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.735
NoneNo IPR availableSUPERFAMILYSSF53187Zn-dependent exopeptidasescoord: 120..417
NoneNo IPR availableSUPERFAMILYSSF54897Protease propeptides/inhibitorscoord: 22..104
IPR000834Peptidase M14, carboxypeptidase APRINTSPR00765CRBOXYPTASEAcoord: 168..182
score: 37.44
coord: 304..317
score: 32.28
coord: 147..159
score: 41.42
coord: 250..258
score: 73.5
IPR000834Peptidase M14, carboxypeptidase ASMARTSM00631zn_carbcoord: 121..405
e-value: 7.5E-100
score: 347.7
IPR000834Peptidase M14, carboxypeptidase APFAMPF00246Peptidase_M14coord: 128..408
e-value: 9.3E-72
score: 242.2
IPR036990Metallocarboxypeptidase-like, propeptideGENE3DG3DSA:3.30.70.340coord: 18..102
e-value: 1.0E-9
score: 40.2
IPR003146Carboxypeptidase, activation peptidePFAMPF02244Propep_M14coord: 23..96
e-value: 1.4E-8
score: 35.0