Voltage-dependent N-type calcium channel subunit alpha

Overview
NameVoltage-dependent N-type calcium channel subunit alpha
Smed IDSMED30026282
Length (bp)377
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Voltage-dependent N-type calcium channel subunit alpha (SMED30026282) t-SNE clustered cells

Violin plots show distribution of expression levels for Voltage-dependent N-type calcium channel subunit alpha (SMED30026282) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30026282

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30026282h1SMcG0020675 Contig6657newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30026282h1SMcG0020675 Contig6657uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30026282h1SMcG0020675 Contig6657newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30026282h1SMcG0020675 Contig6657uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
prepharyngeal regionSMED30026282h1SMcG0020675 SmedASXL_014139SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30026282h1SMcG0020675 SmedASXL_014139SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30026282h1SMcG0020675 dd_Smed_v4_15815_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026282h1SMcG0020675 dd_Smed_v4_15815_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30026282h1SMcG0020675 dd_Smed_v6_15815_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 59.3066 bits (142), Expect = 8.337e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 8.337e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 59.3066 bits (142), Expect = 8.475e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 8.475e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 59.3066 bits (142), Expect = 8.615e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 8.615e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 59.3066 bits (142), Expect = 8.615e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 8.615e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1B (calcium voltage-gated channel subunit alpha1 B [Source:HGNC Symbol;Acc:HGNC:1389])

HSP 1 Score: 59.3066 bits (142), Expect = 9.831e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 9.831e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 47.3654 bits (111), Expect = 1.867e-12
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN + G+ +N  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  454 TNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSG 488          

HSP 2 Score: 40.817 bits (94), Expect = 1.867e-12
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++LKE+
Sbjct:  489 EFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKED 534          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 46.9802 bits (110), Expect = 1.897e-12
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN + G+ +N  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  498 TNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSG 532          

HSP 2 Score: 40.817 bits (94), Expect = 1.897e-12
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++LKE+
Sbjct:  533 EFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKED 578          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76])

HSP 1 Score: 43.8986 bits (102), Expect = 1.986e-12
Identity = 21/34 (61.76%), Postives = 27/34 (79.41%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A G  W   Y+V+LV++GSFF+LNL+LGVLSG
Sbjct:  336 NDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSG 369          

HSP 2 Score: 43.8986 bits (102), Expect = 1.986e-12
Identity = 20/47 (42.55%), Postives = 29/47 (61.70%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EF+KERE+   R  F K R+ + ++ +L+GYLDWI    +I    ED
Sbjct:  370 EFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNED 416          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45])

HSP 1 Score: 46.9802 bits (110), Expect = 2.198e-12
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN + G+ +N  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  446 TNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSG 480          

HSP 2 Score: 40.817 bits (94), Expect = 2.198e-12
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++LKE+
Sbjct:  481 EFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKED 526          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76])

HSP 1 Score: 43.8986 bits (102), Expect = 2.296e-12
Identity = 21/34 (61.76%), Postives = 27/34 (79.41%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A G  W   Y+V+LV++GSFF+LNL+LGVLSG
Sbjct:  336 NDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSG 369          

HSP 2 Score: 43.8986 bits (102), Expect = 2.296e-12
Identity = 20/47 (42.55%), Postives = 29/47 (61.70%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EF+KERE+   R  F K R+ + ++ +L+GYLDWI    +I    ED
Sbjct:  370 EFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNED 416          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307319)

HSP 1 Score: 56.9954 bits (136), Expect = 1.048e-16
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERE+VE RQ F+K+R+ + ++ EL GY++WI    E++L EE
Sbjct:  315 EFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEE 360          

HSP 2 Score: 45.8246 bits (107), Expect = 1.048e-16
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G+ +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  280 TNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSG 314          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307313)

HSP 1 Score: 56.9954 bits (136), Expect = 1.057e-16
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERE+VE RQ F+K+R+ + ++ EL GY++WI    E++L EE
Sbjct:  315 EFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEE 360          

HSP 2 Score: 45.8246 bits (107), Expect = 1.057e-16
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G+ +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  280 TNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSG 314          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307320)

HSP 1 Score: 56.9954 bits (136), Expect = 1.066e-16
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERE+VE RQ F+K+R+ + ++ EL GY++WI    E++L EE
Sbjct:  315 EFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEE 360          

HSP 2 Score: 45.8246 bits (107), Expect = 1.066e-16
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G+ +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  280 TNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSG 314          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307316)

HSP 1 Score: 56.9954 bits (136), Expect = 1.066e-16
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERE+VE RQ F+K+R+ + ++ EL GY++WI    E++L EE
Sbjct:  315 EFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEE 360          

HSP 2 Score: 45.8246 bits (107), Expect = 1.066e-16
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G+ +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  280 TNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSG 314          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307317)

HSP 1 Score: 56.9954 bits (136), Expect = 1.074e-16
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEE 353
            EFAKERE+VE RQ F+K+R+ + ++ EL GY++WI    E++L EE
Sbjct:  315 EFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEE 360          

HSP 2 Score: 45.8246 bits (107), Expect = 1.074e-16
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G+ +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  280 TNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSG 314          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 63.929 bits (154), Expect = 2.876e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  339 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 385          

HSP 2 Score: 54.6842 bits (130), Expect = 2.876e-21
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  304 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 338          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 63.929 bits (154), Expect = 2.876e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  339 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 385          

HSP 2 Score: 54.6842 bits (130), Expect = 2.876e-21
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  304 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 338          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 62.7734 bits (151), Expect = 1.070e-20
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  346 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 392          

HSP 2 Score: 53.9138 bits (128), Expect = 1.070e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  311 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 345          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ba (calcium channel, voltage-dependent, N type, alpha 1B subunit, a [Source:ZFIN;Acc:ZDB-GENE-060531-44])

HSP 1 Score: 62.3882 bits (150), Expect = 1.750e-20
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  351 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEED 397          

HSP 2 Score: 53.1434 bits (126), Expect = 1.750e-20
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  317 NDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 350          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ba (calcium channel, voltage-dependent, N type, alpha 1B subunit, a [Source:ZFIN;Acc:ZDB-GENE-060531-44])

HSP 1 Score: 62.3882 bits (150), Expect = 1.980e-20
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  351 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEED 397          

HSP 2 Score: 53.1434 bits (126), Expect = 1.980e-20
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  317 NDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 350          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 55.4546 bits (132), Expect = 6.746e-18
Identity = 24/47 (51.06%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GY  WI+   E++L EE+
Sbjct:  354 EFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEEN 400          

HSP 2 Score: 51.9878 bits (123), Expect = 6.746e-18
Identity = 21/35 (60.00%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFF+LNL+LGVLSG
Sbjct:  319 TNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSG 353          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 55.4546 bits (132), Expect = 7.087e-18
Identity = 24/47 (51.06%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GY  WI+   E++L EE+
Sbjct:  354 EFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEEN 400          

HSP 2 Score: 51.9878 bits (123), Expect = 7.087e-18
Identity = 21/35 (60.00%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFF+LNL+LGVLSG
Sbjct:  319 TNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSG 353          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 55.0694 bits (131), Expect = 7.815e-18
Identity = 24/47 (51.06%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GY  WI+   E++L EE+
Sbjct:  354 EFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEEN 400          

HSP 2 Score: 51.9878 bits (123), Expect = 7.815e-18
Identity = 21/35 (60.00%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFF+LNL+LGVLSG
Sbjct:  319 TNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSG 353          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 55.0694 bits (131), Expect = 8.344e-18
Identity = 24/47 (51.06%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GY  WI+   E++L EE+
Sbjct:  354 EFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEEN 400          

HSP 2 Score: 51.9878 bits (123), Expect = 8.344e-18
Identity = 21/35 (60.00%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFF+LNL+LGVLSG
Sbjct:  319 TNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSG 353          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:Xenbase;Acc:XB-GENE-970920])

HSP 1 Score: 55.0694 bits (131), Expect = 8.702e-18
Identity = 24/47 (51.06%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GY  WI+   E++L EE+
Sbjct:  244 EFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEEN 290          

HSP 2 Score: 51.9878 bits (123), Expect = 8.702e-18
Identity = 21/35 (60.00%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFF+LNL+LGVLSG
Sbjct:  209 TNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSG 243          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 59.6918 bits (143), Expect = 4.937e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 4.937e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 59.6918 bits (143), Expect = 4.937e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 4.937e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 59.6918 bits (143), Expect = 4.939e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 4.939e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 59.6918 bits (143), Expect = 4.978e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 4.978e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1b (calcium channel, voltage-dependent, N type, alpha 1B subunit [Source:MGI Symbol;Acc:MGI:88296])

HSP 1 Score: 59.6918 bits (143), Expect = 5.403e-20
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 5.403e-20
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q05152|CAC1B_RABIT (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Oryctolagus cuniculus OX=9986 GN=CACNA1B PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 3.204e-19
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 3.204e-19
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|O55017|CAC1B_MOUSE (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Mus musculus OX=10090 GN=Cacna1b PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 3.312e-19
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 3.312e-19
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q02294|CAC1B_RAT (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Rattus norvegicus OX=10116 GN=Cacna1b PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 3.594e-19
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 3.594e-19
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q00975|CAC1B_HUMAN (Voltage-dependent N-type calcium channel subunit alpha-1B OS=Homo sapiens OX=9606 GN=CACNA1B PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 4.065e-19
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  358 EFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEED 404          

HSP 2 Score: 54.6842 bits (130), Expect = 4.065e-19
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  323 TNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 357          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|O00555|CAC1A_HUMAN (Voltage-dependent P/Q-type calcium channel subunit alpha-1A OS=Homo sapiens OX=9606 GN=CACNA1A PE=1 SV=3)

HSP 1 Score: 58.5362 bits (140), Expect = 1.132e-18
Identity = 25/54 (46.30%), Postives = 38/54 (70.37%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEEDLPDLQRQ 377
            EFAKERERVE R+ F+K+R+ + ++ EL GY++WI    E++L E++    QR 
Sbjct:  362 EFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRH 415          

HSP 2 Score: 53.9138 bits (128), Expect = 1.132e-18
Identity = 21/35 (60.00%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            +N A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  327 SNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 361          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A4W4G6H3 (Voltage-dependent N-type calcium channel subunit alpha OS=Electrophorus electricus OX=8005 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 4.313e-18
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  352 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 398          

HSP 2 Score: 54.299 bits (129), Expect = 4.313e-18
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  317 TNDASGNTWNWLYFIPLIILGSFFMLNLVLGVLSG 351          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A2I4CHI6 (Voltage-dependent N-type calcium channel subunit alpha OS=Austrofundulus limnaeus OX=52670 GN=LOC106528746 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.959e-18
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  350 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEED 396          

HSP 2 Score: 54.6842 bits (130), Expect = 4.959e-18
Identity = 22/34 (64.71%), Postives = 28/34 (82.35%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N ATG  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  316 NDATGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 349          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A3B4D8V7 (Voltage-dependent N-type calcium channel subunit alpha OS=Pygocentrus nattereri OX=42514 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 5.306e-18
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  343 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 389          

HSP 2 Score: 55.4546 bits (132), Expect = 5.306e-18
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  308 TNDASGNAWNWLYFIPLIIIGSFFMLNLVLGVLSG 342          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A3B4D981 (Voltage-dependent N-type calcium channel subunit alpha OS=Pygocentrus nattereri OX=42514 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 5.340e-18
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  348 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 394          

HSP 2 Score: 55.4546 bits (132), Expect = 5.340e-18
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  313 TNDASGNAWNWLYFIPLIIIGSFFMLNLVLGVLSG 347          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Match: A0A4W4G2T6 (Voltage-dependent N-type calcium channel subunit alpha OS=Electrophorus electricus OX=8005 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.528e-18
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  349 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 395          

HSP 2 Score: 54.299 bits (129), Expect = 5.528e-18
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  314 TNDASGNTWNWLYFIPLIILGSFFMLNLVLGVLSG 348          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 62.3882 bits (150), Expect = 4.259e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  341 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 387          

HSP 2 Score: 55.0694 bits (131), Expect = 4.259e-21
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  306 TNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 340          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 62.3882 bits (150), Expect = 4.365e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  341 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 387          

HSP 2 Score: 55.0694 bits (131), Expect = 4.365e-21
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  306 TNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 340          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 62.3882 bits (150), Expect = 4.366e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  341 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 387          

HSP 2 Score: 55.0694 bits (131), Expect = 4.366e-21
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  306 TNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 340          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1bb (calcium channel, voltage-dependent, N type, alpha 1B subunit, b [Source:ZFIN;Acc:ZDB-GENE-090514-4])

HSP 1 Score: 62.3882 bits (150), Expect = 4.438e-21
Identity = 29/47 (61.70%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E+LL EED
Sbjct:  341 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEED 387          

HSP 2 Score: 55.0694 bits (131), Expect = 4.438e-21
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  306 TNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 340          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1ba (calcium channel, voltage-dependent, N type, alpha 1B subunit, a [Source:ZFIN;Acc:ZDB-GENE-060531-44])

HSP 1 Score: 62.7734 bits (151), Expect = 1.259e-20
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  341 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEED 387          

HSP 2 Score: 53.1434 bits (126), Expect = 1.259e-20
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  307 NDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 340          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004641.1 (pep scaffold:Pmarinus_7.0:GL484016:2326:11357:-1 gene:ENSPMAG00000004230.1 transcript:ENSPMAT00000004641.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 4.808e-12
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R+ F+K+R+ + +  EL GYL+WI    E++L+EED
Sbjct:  153 EFAKERERVENRRAFLKLRRQQQLDRELNGYLEWICKAEEVMLEEED 199          

HSP 2 Score: 33.113 bits (74), Expect = 4.808e-12
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = 2
Query:   74 ATGTPWNNFYYVSLVLVGSFFMLNLILGV 160
            A G  WN  Y++ L+++GSFF LN+  G+
Sbjct:  121 ALGNTWNWLYFIPLIIIGSFFTLNICHGL 149          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003253.1 (pep scaffold:Pmarinus_7.0:GL478330:685:47673:1 gene:ENSPMAG00000002915.1 transcript:ENSPMAT00000003253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 3.363e-10
Identity = 26/48 (54.17%), Postives = 37/48 (77.08%), Query Frame = 3
Query:  213 REFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            REFAKERERVE R+ F+K+R+ + ++ EL GY++WI    E++L EED
Sbjct:    4 REFAKERERVENRRAFLKLRRQQQIERELNGYMEWICKAEEVMLAEED 51          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO46991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU9])

HSP 1 Score: 45.0542 bits (105), Expect = 1.295e-11
Identity = 20/34 (58.82%), Postives = 26/34 (76.47%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A G  W   Y+V+L++ GSFF+LNL+LGVLSG
Sbjct:  312 NDAIGNSWPWLYFVTLIIWGSFFVLNLVLGVLSG 345          

HSP 2 Score: 38.891 bits (89), Expect = 1.295e-11
Identity = 17/41 (41.46%), Postives = 25/41 (60.98%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEI 338
            EFAKE+ R +K   F K+R+ +++     GYLDWI    +I
Sbjct:  346 EFAKEKARAQKSGEFQKLREKQLVDDAYHGYLDWISQAEDI 386          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ba (voltage-dependent N-type calcium channel subunit alpha-1B [Source:NCBI gene;Acc:101157063])

HSP 1 Score: 62.7734 bits (151), Expect = 1.366e-20
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI    E++L EED
Sbjct:  350 EFAKERERVEKRQEFLKLRRQQQIEKELTGYLEWICKAEEVMLAEED 396          

HSP 2 Score: 53.1434 bits (126), Expect = 1.366e-20
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 2
Query:   68 NFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            N A+G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  316 NDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 349          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1aa (voltage-dependent P/Q-type calcium channel subunit alpha-1A [Source:NCBI gene;Acc:101170273])

HSP 1 Score: 54.6842 bits (130), Expect = 1.474e-18
Identity = 23/47 (48.94%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R  F+K+R+ + ++ EL GYL+WI    E++L +++
Sbjct:  258 EFAKERERVENRSEFLKLRRQQQIERELNGYLEWICKAEEVILADDE 304          

HSP 2 Score: 54.299 bits (129), Expect = 1.474e-18
Identity = 21/35 (60.00%), Postives = 30/35 (85.71%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            +N A+G+ WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  223 SNDASGSAWNWMYFIPLIIIGSFFMLNLVLGVLSG 257          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1aa (voltage-dependent P/Q-type calcium channel subunit alpha-1A [Source:NCBI gene;Acc:101170273])

HSP 1 Score: 54.6842 bits (130), Expect = 1.535e-18
Identity = 23/47 (48.94%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R  F+K+R+ + ++ EL GYL+WI    E++L +++
Sbjct:  258 EFAKERERVENRSEFLKLRRQQQIERELNGYLEWICKAEEVILADDE 304          

HSP 2 Score: 54.299 bits (129), Expect = 1.535e-18
Identity = 21/35 (60.00%), Postives = 30/35 (85.71%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            +N A+G+ WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  223 SNDASGSAWNWMYFIPLIIIGSFFMLNLVLGVLSG 257          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1aa (voltage-dependent P/Q-type calcium channel subunit alpha-1A [Source:NCBI gene;Acc:101170273])

HSP 1 Score: 54.6842 bits (130), Expect = 1.626e-18
Identity = 23/47 (48.94%), Postives = 35/47 (74.47%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKEED 356
            EFAKERERVE R  F+K+R+ + ++ EL GYL+WI    E++L +++
Sbjct:  258 EFAKERERVENRSEFLKLRRQQQIERELNGYLEWICKAEEVILADDE 304          

HSP 2 Score: 54.299 bits (129), Expect = 1.626e-18
Identity = 21/35 (60.00%), Postives = 30/35 (85.71%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            +N A+G+ WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  223 SNDASGSAWNWMYFIPLIIIGSFFMLNLVLGVLSG 257          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1bb (voltage-dependent N-type calcium channel subunit alpha-1B [Source:NCBI gene;Acc:101167885])

HSP 1 Score: 55.0694 bits (131), Expect = 3.720e-18
Identity = 23/35 (65.71%), Postives = 30/35 (85.71%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWI 320
            EFAKERERVEKRQ F+K+R+ + ++ EL GYL+WI
Sbjct:  354 EFAKERERVEKRQEFLKLRRQQQIERELTGYLEWI 388          

HSP 2 Score: 52.7582 bits (125), Expect = 3.720e-18
Identity = 21/35 (60.00%), Postives = 27/35 (77.14%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN   G  WN  Y++ L+++GSFFMLNL+LGVLSG
Sbjct:  319 TNDVVGNTWNWLYFIPLIIIGSFFMLNLVLGVLSG 353          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 58.151 bits (139), Expect = 9.941e-18
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKE 350
            EFAKERERVEKR+ F+K+R+ +  + E  GY++WI+   E+LL E
Sbjct:  220 EFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNE 264          

HSP 2 Score: 47.7506 bits (112), Expect = 9.941e-18
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  185 TNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSG 219          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.142e-17
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKE 350
            EFAKERERVEKR+ F+K+R+ +  + E  GY++WI+   E+LL E
Sbjct:  256 EFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNE 300          

HSP 2 Score: 47.3654 bits (111), Expect = 1.142e-17
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  221 TNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSG 255          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.151e-17
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKE 350
            EFAKERERVEKR+ F+K+R+ +  + E  GY++WI+   E+LL E
Sbjct:  256 EFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNE 300          

HSP 2 Score: 47.3654 bits (111), Expect = 1.151e-17
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  221 TNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSG 255          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.180e-17
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKE 350
            EFAKERERVEKR+ F+K+R+ +  + E  GY++WI+   E+LL E
Sbjct:  278 EFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNE 322          

HSP 2 Score: 47.3654 bits (111), Expect = 1.180e-17
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  243 TNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSG 277          
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.199e-17
Identity = 24/45 (53.33%), Postives = 34/45 (75.56%), Query Frame = 3
Query:  216 EFAKERERVEKRQTFIKVRQTKIMQMELEGYLDWIEAYGEILLKE 350
            EFAKERERVEKR+ F+K+R+ +  + E  GY++WI+   E+LL E
Sbjct:  293 EFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNE 337          

HSP 2 Score: 47.3654 bits (111), Expect = 1.199e-17
Identity = 22/35 (62.86%), Postives = 28/35 (80.00%), Query Frame = 2
Query:   65 TNFATGTPWNNFYYVSLVLVGSFFMLNLILGVLSG 169
            TN A G  +N  Y+V L+++GSFFMLNL+LGVLSG
Sbjct:  258 TNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSG 292          
The following BLAST results are available for this feature:
BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CACNA1B8.337e-2055.32calcium voltage-gated channel subunit alpha1 B [So... [more]
CACNA1B8.475e-2055.32calcium voltage-gated channel subunit alpha1 B [So... [more]
CACNA1B8.615e-2055.32calcium voltage-gated channel subunit alpha1 B [So... [more]
CACNA1B8.615e-2055.32calcium voltage-gated channel subunit alpha1 B [So... [more]
CACNA1B9.831e-2055.32calcium voltage-gated channel subunit alpha1 B [So... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-21.867e-1257.14High voltage activated calcium channel alpha-1 sub... [more]
unc-21.897e-1257.14High voltage activated calcium channel alpha-1 sub... [more]
egl-191.986e-1261.76Voltage-dependent L-type calcium channel subunit a... [more]
unc-22.198e-1257.14High voltage activated calcium channel alpha-1 sub... [more]
egl-192.296e-1261.76Voltage-dependent L-type calcium channel subunit a... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cac1.048e-1652.17gene:FBgn0263111 transcript:FBtr0307319[more]
cac1.057e-1652.17gene:FBgn0263111 transcript:FBtr0307313[more]
cac1.066e-1652.17gene:FBgn0263111 transcript:FBtr0307320[more]
cac1.066e-1652.17gene:FBgn0263111 transcript:FBtr0307316[more]
cac1.074e-1652.17gene:FBgn0263111 transcript:FBtr0307317[more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1bb2.876e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1bb2.876e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1bb1.070e-2061.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1ba1.750e-2059.57calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1ba1.980e-2059.57calcium channel, voltage-dependent, N type, alpha ... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mrps116.746e-1851.06mitochondrial ribosomal protein S11 [Source:Xenbas... [more]
mrps117.087e-1851.06mitochondrial ribosomal protein S11 [Source:Xenbas... [more]
mrps117.815e-1851.06mitochondrial ribosomal protein S11 [Source:Xenbas... [more]
mrps118.344e-1851.06mitochondrial ribosomal protein S11 [Source:Xenbas... [more]
mrps118.702e-1851.06mitochondrial ribosomal protein S11 [Source:Xenbas... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cacna1b4.937e-2055.32calcium channel, voltage-dependent, N type, alpha ... [more]
Cacna1b4.937e-2055.32calcium channel, voltage-dependent, N type, alpha ... [more]
Cacna1b4.939e-2055.32calcium channel, voltage-dependent, N type, alpha ... [more]
Cacna1b4.978e-2055.32calcium channel, voltage-dependent, N type, alpha ... [more]
Cacna1b5.403e-2055.32calcium channel, voltage-dependent, N type, alpha ... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q05152|CAC1B_RABIT3.204e-1955.32Voltage-dependent N-type calcium channel subunit a... [more]
sp|O55017|CAC1B_MOUSE3.312e-1955.32Voltage-dependent N-type calcium channel subunit a... [more]
sp|Q02294|CAC1B_RAT3.594e-1955.32Voltage-dependent N-type calcium channel subunit a... [more]
sp|Q00975|CAC1B_HUMAN4.065e-1955.32Voltage-dependent N-type calcium channel subunit a... [more]
sp|O00555|CAC1A_HUMAN1.132e-1846.30Voltage-dependent P/Q-type calcium channel subunit... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4W4G6H34.313e-1861.70Voltage-dependent N-type calcium channel subunit a... [more]
A0A2I4CHI64.959e-1859.57Voltage-dependent N-type calcium channel subunit a... [more]
A0A3B4D8V75.306e-1861.70Voltage-dependent N-type calcium channel subunit a... [more]
A0A3B4D9815.340e-1861.70Voltage-dependent N-type calcium channel subunit a... [more]
A0A4W4G2T65.528e-1861.70Voltage-dependent N-type calcium channel subunit a... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1bb4.259e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1bb4.365e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1bb4.366e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1bb4.438e-2161.70calcium channel, voltage-dependent, N type, alpha ... [more]
cacna1ba1.259e-2059.57calcium channel, voltage-dependent, N type, alpha ... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000004641.14.808e-1255.32pep scaffold:Pmarinus_7.0:GL484016:2326:11357:-1 g... [more]
ENSPMAT00000003253.13.363e-1054.17pep scaffold:Pmarinus_7.0:GL478330:685:47673:1 gen... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO469911.295e-1158.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1ba1.366e-2059.57voltage-dependent N-type calcium channel subunit a... [more]
cacna1aa1.474e-1848.94voltage-dependent P/Q-type calcium channel subunit... [more]
cacna1aa1.535e-1848.94voltage-dependent P/Q-type calcium channel subunit... [more]
cacna1aa1.626e-1848.94voltage-dependent P/Q-type calcium channel subunit... [more]
cacna1bb3.720e-1865.71voltage-dependent N-type calcium channel subunit a... [more]
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BLAST of Voltage-dependent N-type calcium channel subunit alpha vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028279.19.941e-1853.33SMESG000028279.1[more]
SMESG000028279.11.142e-1753.33SMESG000028279.1[more]
SMESG000028279.11.151e-1753.33SMESG000028279.1[more]
SMESG000028279.11.180e-1753.33SMESG000028279.1[more]
SMESG000028279.11.199e-1753.33SMESG000028279.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026282 ID=SMED30026282|Name=Voltage-dependent N-type calcium channel subunit alpha|organism=Schmidtea mediterranea sexual|type=transcript|length=377bp
TCGGTTAACTTTTCGGAGAGAATTTTCTATATATTTATTTTTGTAAATGA
TCAATTGAATTTAGACTAATTTTGCGACTGGAACTCCTTGGAACAATTTT
TATTACGTGAGTTTGGTTTTGGTTGGCTCATTTTTTATGTTAAACTTGAT
TCTGGGAGTTTTGAGTGGGTGAGTTATTTTCTTGCGATTGATGAACGATT
TATTCTTCTTTTAGGGAATTTGCCAAAGAAAGAGAAAGAGTTGAAAAGCG
ACAAACCTTTATTAAAGTTCGACAAACTAAAATCATGCAGATGGAATTGG
AAGGCTATCTAGATTGGATTGAGGCCTATGGAGAGATTCTGCTAAAGGAA
GAAGATCTTCCAGACCTTCAACGGCAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
Vocabulary: biological process
TermDefinition
GO:0034765regulation of ion transmembrane transport
GO:0055085transmembrane transport
GO:0070588calcium ion transmembrane transport
GO:0006811ion transport
GO:0006816calcium ion transport
Vocabulary: molecular function
TermDefinition
GO:0005216ion channel activity
GO:0005244voltage-gated ion channel activity
GO:0005245voltage-gated calcium channel activity
GO:0005262calcium channel activity
Vocabulary: cellular component
TermDefinition
GO:0005891voltage-gated calcium channel complex
GO:0016020membrane
GO:0016021integral component of membrane