Pre-mRNA-splicing factor ISY1

Overview
NamePre-mRNA-splicing factor ISY1
Smed IDSMED30026278
Length (bp)855
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pre-mRNA-splicing factor ISY1 (SMED30026278) t-SNE clustered cells

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ISY1 (SMED30026278) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pre-mRNA-splicing factor ISY1 (SMED30026278) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ISY1 (SMED30026278) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026278

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30026278SMESG000047126.1 SMESG000004187.1 SmedASXL_005364SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026278SMESG000047126.1 SMESG000004187.1 Contig41435newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026278SMESG000047126.1 SMESG000004187.1 Contig41435uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v6_8243_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30026278SMESG000047126.1 SMESG000004187.1 OX_Smed_1.0.16288ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30026278SMESG000047126.1 SMESG000004187.1 dd_Smed_v4_8243_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A5K4F520 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 6.870e-44
Identity = 74/200 (37.00%), Postives = 121/200 (60.50%), Query Frame = 1 
Query:  157 ETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELK 756
            +T  VLV+  FD  L K+F N++V++K+++++YGNGQ VGF+EE+  ++GDW +  T+G L FD+   A++W  SDP+ +Q DWLD  EI+++PL        Y  + LFN   INE+ F +++LP ++ ++   G   FI+ T  +E  RG     +++I+ WP  D  + W  S +   +RN+QD      TI+  +K
Sbjct:    8 DTPAVLVIFQFDKELCKTFNNSVVRQKSVMKSYGNGQSVGFAEEVVCLDGDWKRNTTVGFLHFDSAMAAQRWLISDPIVRQHDWLDDAEIWIVPLCTEIRLWNYLQLSLFNS--INEDNFKNQYLPKFEESVSKFGGVPFISSTPYIEVPRGLKEIDYLIITEWPDEDSSLKWNRSHEAEELRNMQDSFSKSSTILAMIK 205
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: Q5DGC4 (SJCHGC02082 protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002134 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.772e-40
Identity = 69/203 (33.99%), Postives = 121/203 (59.61%), Query Frame = 1 
Query:  148 SQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELK 756
            S  +   VLV+  FD  L + F N +V++K+++++YG+G   GF++E+  ++GDW +  T+G L FD+    ++W  SDP+  Q DWLD VEI+++PL     + +Y  + LFN  L++E+ F  ++LP ++ ++   G   FI+ T  +E  RG     +++IS WP  D + NW  S + N ++N+QD I    T++  ++
Sbjct:    2 SNTDNSPVLVIFQFDRELCRRFNNDVVRQKSVMKSYGHGHSAGFADEVIYLDGDWKRNITVGFLHFDSAVAVQRWINSDPIFHQHDWLDDVEIWIVPLCTEMKQWKYLQLSLFN--LVDEKNFKDRYLPKFEESVSKFGGIPFISSTSYIEVLRGLKETDYLIISEWPDEDTFSNWNQSHEANELKNMQDTISKSSTMLAMIR 202
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A095B0K2 (Uncharacterized protein (Fragment) OS=Schistosoma haematobium OX=6185 GN=MS3_09085 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.514e-36
Identity = 63/180 (35.00%), Postives = 108/180 (60.00%), Query Frame = 1 
Query:  217 NTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELK 756
            N++V++K+++++YGNGQ VGF++E+  ++GDW +  TIG L FD+   A++W  SDP+ +Q DWLD  EI+++PL        Y  + LFN   INE+ F +++LP ++ ++   G   FI+ T  +E  RG     +++I+ WP  D    W  S +   +RN+Q+      TI+  ++
Sbjct:    2 NSVVRQKSVMKSYGNGQSVGFADEVVCLDGDWKRNTTIGFLHFDSAVAAQRWLISDPIFRQHDWLDDAEIWIVPLCTEIRPWNYLQLSLFNS--INEDNFKNQYLPKFEESVSKFGGVPFISSTSYIEVPRGLKEIDYLIITGWPDDDSSFKWNQSHEAEELRNMQESFSKSSTILAMIR 179
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A183W8Q2 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS8505 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.340e-36
Identity = 64/176 (36.36%), Postives = 109/176 (61.93%), Query Frame = 1 
Query:  157 ETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNS 684
            E   V V+  FD  L K F N++V++K+++R+YGNGQ VGF+EE+  + GDW +  TIG+L+FD+++ A++W  SDP+ +Q DWLD  EI+++PL+     K ++ ++L    + NE++F H++LP+Y   +   G   F+  T  VE  R      +++++ WP  +  + W  S
Sbjct:    7 EITSVAVIFQFDGALCKKFNNSIVRQKSVMRSYGNGQPVGFAEEVICLAGDWKRNKTIGVLQFDSIEAAQRWFNSDPIFRQHDWLDDAEIWIVPLVSEI--KPWKYLQLSLLKITNEDEFKHQYLPNYGELVTSFGGVPFVCSTSDVEVRRRIKEIDYLIMTEWPDEESALKWSRS 180
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A074Z948 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_09720 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 6.756e-36
Identity = 69/197 (35.03%), Postives = 115/197 (58.38%), Query Frame = 1 
Query:  166 EVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELK 756
            ++L+L +FD  LTKSF N +V +KN++R YGNG+ VG++E++  +EG W    T+GLL+FD+ + A +W  SDP  ++ DWLD  +++V+PL +    + Y   EL    + +  QF ++FLP ++ AI   G    +  T  V+ WRG H+  HV +S WP+      W  S++   +++ Q  +  G   I  ++
Sbjct:   10 KILLLYIFDNQLTKSFNNRLVSQKNVMRTYGNGKPVGYAEDMLCVEGIWRPNMTMGLLQFDSEETALRWRQSDPQFRKSDWLDDHDLWVLPLHQEL--ETYACFELDMFKVPDPNQFENEFLPKWEEAIRKAGGTPGVVSTGTVKIWRGMHDVDHVFLSQWPTELVASEWHRSDEAMQLKSHQPPVANGCMTIARMR 204
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Match: SMESG000004187.1 (SMESG000004187.1)

HSP 1 Score: 459.914 bits (1182), Expect = 2.764e-166
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 1 
Query:  106 MSHISEPGASSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 765
            MSHISEPGA SCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL
Sbjct:    1 MSHISEPGAGSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 220
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Match: SMESG000047126.1 (SMESG000047126.1)

HSP 1 Score: 459.529 bits (1181), Expect = 3.883e-166
Identity = 218/220 (99.09%), Postives = 219/220 (99.55%), Query Frame = 1 
Query:  106 MSHISEPGASSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 765
            MSHISEPGA SCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQM+GFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL
Sbjct:    1 MSHISEPGAGSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMIGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 220
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Match: SMESG000047126.1 (SMESG000047126.1)

HSP 1 Score: 405.986 bits (1042), Expect = 3.025e-145
Identity = 192/198 (96.97%), Postives = 195/198 (98.48%), Query Frame = 1 
Query:  106 MSHISEPGASSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNH 699
            MSHISEPGA SCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQM+GFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNS + N+
Sbjct:    1 MSHISEPGAGSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYGNGQMIGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSGNSNN 198
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Match: SMESG000007291.1 (SMESG000007291.1)

HSP 1 Score: 113.235 bits (282), Expect = 5.415e-30
Identity = 62/214 (28.97%), Postives = 120/214 (56.07%), Query Frame = 1 
Query:  139 CGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNYG---NGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKA-FPKKEYQVMELFNCDL-INEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 765
            C + + +  E  V ILF  +  +++ N  VKEK +I  Y     GQ+VGF+  ++ IEG+W  K ++G+ RF T  DAK W  SD   +QQD+L  V+I++ P++     +K Y +M++ + ++   ++ F HK+LP+ +++   +G   F+   +    WRG     H +++ +PS+ ++  +  ++++  + + +     G  I+ E++TKL
Sbjct:   12 CNEERFKWKECFVFILFPKSRAQAYRNAAVKEKAVISRYSIHKPGQIVGFAYHVQAIEGNWNCKLSVGVFRFATYNDAKMWLESDNEFRQQDFLTNVDIWIAPIINEDLTRKGYAIMQIISQEVCFFKDIFLHKYLPNEEASTINHGGHPFVINHENPYCWRGVPPPGHTIVNQFPSIMNFERYLAADEYKSLADYRKFTGNGFMIVAEMETKL 225
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Match: SMESG000062647.1 (SMESG000062647.1)

HSP 1 Score: 113.62 bits (283), Expect = 7.407e-30
Identity = 60/204 (29.41%), Postives = 113/204 (55.39%), Query Frame = 1 
Query:  166 EVLVLILFDATLTKSFCNTMVKEKNIIRNY---GNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQDWLDQVEIYVMPLLKA-FPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAIDGNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIRNLQDEICVGETIIVELKTKL 765
            E  VLI+F  +  +++ N  VKEK +I  Y     G+ VGF+ ++KVIEG W  + +IG L+F + +DA  W  SD   +QQD+L  V+I+++P+ +     K Y ++++ +  +   + F+HK+LP+ ++A+  +    F+        WRG+    H++I+ +PS D + N+ + + +  +   + +   G  I+ +L   L
Sbjct:   53 ECFVLIIFPESRGQAYRNAAVKEKAVISRYSIHAPGKNVGFAYQVKVIEGYWDTQLSIGALKFASYRDASMWLESDNAFRQQDFLTHVDIWIVPINREDMSHKGYPILQMVSQKVCYPDIFFHKYLPAEEAAVTSHEGVPFVVNHPTPYAWRGKKPPGHIVINQFPSCDAFNNYLSVDSYKPLAEYRKQTGEGFMIVAKLNIDL 256
The following BLAST results are available for this feature:
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4F5206.870e-4437.00Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
Q5DGC41.772e-4033.99SJCHGC02082 protein OS=Schistosoma japonicum OX=61... [more]
A0A095B0K21.514e-3635.00Uncharacterized protein (Fragment) OS=Schistosoma ... [more]
A0A183W8Q22.340e-3636.36Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A074Z9486.756e-3635.03Uncharacterized protein OS=Opisthorchis viverrini ... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004187.12.764e-16699.55SMESG000004187.1[more]
SMESG000047126.13.883e-16699.09SMESG000047126.1[more]
SMESG000047126.13.025e-14596.97SMESG000047126.1[more]
SMESG000007291.15.415e-3028.97SMESG000007291.1[more]
SMESG000062647.17.407e-3029.41SMESG000062647.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026278 ID=SMED30026278|Name=Pre-mRNA-splicing factor ISY1|organism=Schmidtea mediterranea sexual|type=transcript|length=855bp
TGAGTGTTTAATTGACTCGAGTGCTTCAACGCCACGGTTACAGTTGCTAT
GTTCATTTTAATTTCATAAACTTGGGTTTTAAATTCTTTTTATTTTTCTG
CGAAAATGTCTCATATTTCTGAACCAGGTGCTAGCAGTTGTGGAAAATCT
CAAGGTGAAACTGGTGAAGTTTTAGTTTTAATATTATTTGATGCAACTCT
TACAAAATCATTTTGTAACACAATGGTCAAGGAAAAGAATATTATTAGAA
ATTATGGAAATGGGCAAATGGTTGGATTTAGTGAAGAAATTAAAGTTATT
GAAGGAGATTGGCCGCAAAAATATACGATAGGATTATTAAGATTTGACAC
AATGAAAGACGCCAAAAAATGGAATTTTAGTGATCCTTTAACAAAACAAC
AAGACTGGCTAGATCAAGTGGAAATTTATGTCATGCCTTTACTTAAGGCT
TTTCCAAAAAAGGAATATCAGGTTATGGAACTTTTCAACTGTGATTTAAT
CAACGAGGAACAGTTTTACCACAAATTTCTACCTTCATATAAGTCTGCAA
TTGATGGAAATGGATGTGAAAACTTCATAGCTTGCACAGATAAAGTCGAA
AGATGGCGTGGTCGTCATAATTTTTGTCATGTTATGATTTCACTTTGGCC
ATCATTAGATGATTATATGAACTGGAGGAATTCGGAAGATTTTAATCATA
TTAGAAATTTACAAGATGAAATCTGTGTAGGAGAAACTATTATAGTCGAA
TTGAAAACAAAATTGTAAATTTAAATATTTGTGCACCTTTTGTCTTATAT
TCTTGATATTTAAAATATTTAAATTCTATAATTCTTTGTTATAATATAAA
TAAAT
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protein sequence of SMED30026278-orf-1

>SMED30026278-orf-1 ID=SMED30026278-orf-1|Name=SMED30026278-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
MSHISEPGASSCGKSQGETGEVLVLILFDATLTKSFCNTMVKEKNIIRNY
GNGQMVGFSEEIKVIEGDWPQKYTIGLLRFDTMKDAKKWNFSDPLTKQQD
WLDQVEIYVMPLLKAFPKKEYQVMELFNCDLINEEQFYHKFLPSYKSAID
GNGCENFIACTDKVERWRGRHNFCHVMISLWPSLDDYMNWRNSEDFNHIR
NLQDEICVGETIIVELKTKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000350generation of catalytic spliceosome for second transesterification step
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR010753DUF1330
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010753Domain of unknown function DUF1330PFAMPF07045DUF1330coord: 134..215
e-value: 8.2E-5
score: 22.9