vault protein inter alpha trypsin-1

Overview
Namevault protein inter alpha trypsin-1 (AFJ24785.1)
Smed IDSMED30026256
Length (bp)2091
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of vault protein inter alpha trypsin-1 (SMED30026256) t-SNE clustered cells

Violin plots show distribution of expression levels for vault protein inter alpha trypsin-1 (SMED30026256) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of vault protein inter alpha trypsin-1 (SMED30026256) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for vault protein inter alpha trypsin-1 (SMED30026256) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30026256

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 16

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30026256SMESG000053671.1 SMESG000053667.1 Contig5414GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30026256SMESG000053671.1 SMESG000053667.1 Contig5414GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 Contig42668uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 Contig42668newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026256SMESG000053671.1 SMESG000053667.1 Contig51630newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053657.1 SMESG000053638.1 Contig27090uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053657.1 SMESG000053638.1 Contig27090newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 SMESG000053667.1 dd_Smed_v6_1027_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 SMESG000053667.1 dd_Smed_v6_1027_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026256SMESG000053671.1 SMESG000053669.1 SMESG000053667.1 SMESG000053664.1 SMESG000053657.1 SMESG000053638.1 dd_Smed_v4_1027_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Zebrafish
Match: si:ch73-265h17.4 (si:ch73-265h17.4 [Source:ZFIN;Acc:ZDB-GENE-131121-594])

HSP 1 Score: 47.3654 bits (111), Expect = 5.411e-6
Identity = 22/77 (28.57%), Postives = 39/77 (50.65%), Query Frame = 2
Query:  236 DVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWI 466
            D+++ LV  Q  +G W  + ++   +G    E  N+ P ++   VW T+    +L      ++ EW+ VA+KA  WI
Sbjct:   33 DLLLPLVSLQKASGCWELDAALAAVLGKTEDELTNQKPAQVDGSVWATLLALIWLYSWRKEQQVEWQFVARKAAAWI 109          
BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Zebrafish
Match: si:ch73-265h17.5 (si:ch73-265h17.5 [Source:ZFIN;Acc:ZDB-GENE-131121-293])

HSP 1 Score: 46.595 bits (109), Expect = 6.858e-6
Identity = 22/77 (28.57%), Postives = 38/77 (49.35%), Query Frame = 2
Query:  236 DVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWI 466
            D+++ LV  Q  +G W  + ++    G    E  N+ P ++   VW T+    +L      ++ EW+ VA+KA  WI
Sbjct:   10 DLLLPLVSLQKTSGCWELDAALAAVFGKTKDELTNQKPAQVDGSVWATLLALIWLYGWRKGQQVEWQFVAQKAAAWI 86          
BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Match: I1ZID1 (Vault protein inter alpha trypsin-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 5.689e-57
Identity = 121/124 (97.58%), Postives = 123/124 (99.19%), Query Frame = 2
Query:  161 KKASKQNRKSKDXXXXXXXXXXXXXDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            +KASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQ+LVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLL 
Sbjct:  563 EKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQYLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLH 686          
BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Match: A0A368FVD9 (Uncharacterized protein OS=Ancylostoma caninum OX=29170 GN=ANCCAN_17957 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.900e-32
Identity = 52/140 (37.14%), Postives = 78/140 (55.71%), Query Frame = 1
Query: 1315 FIPEGVKLNEEK*ISLVIEPYVKPLIDFMFAGNNLVFQQHSVLLHRAKSTQSWCPTNLPEFITTKNWPASRPDLNPLDYGI*SILEQKALAINHSNV----ANLSQFDQGLGRYYTRNCTIDQWRNRLSACVRNRAGHFE 1722
            F+  GVK+N++  +  +++  +KP     F      FQQ S   H+A   Q  C   LP+FI + +WP++ PDLNPLD+ + S++E KA +I H N+    A L++  Q +   Y R  T D +  RL  CVR + GHFE
Sbjct:  124 FVDIGVKINKDIYLKSILDDVLKPWSTSHFGSRQWCFQQDSAPAHKANVVQERCRRELPDFIASGDWPSNSPDLNPLDFSVWSVVESKACSIRHQNLEELKAALTKAWQEMDSDYLR-MTCDAFMKRLRRCVRAKGGHFE 262          

HSP 2 Score: 70.8626 bits (172), Expect = 2.900e-32
Identity = 35/75 (46.67%), Postives = 45/75 (60.00%), Query Frame = 2
Query: 1085 KCKQLLSRYDLEAFKNILFSDENIFTVEAKCNCQNM*LLAPSVQQIPPGKGYRERTQHPASVMVWAGVSHQGKTP 1309
            +CK+LL R    + +NI+FSDE IFTVE   N QN  +LA  ++Q         +T HP SVMVW G +  GKTP
Sbjct:   47 RCKKLLKRSAEGSHRNIVFSDEKIFTVELVVNSQNNRVLAKDIEQALSRGKIVGKTAHPTSVMVWGGTTSDGKTP 121          
BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Match: A0A368H7I1 (Uncharacterized protein OS=Ancylostoma caninum OX=29170 GN=ANCCAN_02618 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.009e-31
Identity = 52/140 (37.14%), Postives = 79/140 (56.43%), Query Frame = 1
Query: 1315 FIPEGVKLNEEK*ISLVIEPYVKPLIDFMFAGNNLVFQQHSVLLHRAKSTQSWCPTNLPEFITTKNWPASRPDLNPLDYGI*SILEQKALAINHSNV----ANLSQFDQGLGRYYTRNCTIDQWRNRLSACVRNRAGHFE 1722
            F+  GVK+N++  +  +++  +KP     F      FQQ S   H+A   Q WC   +P+FI   +WP++ P+LNPLD+ + S++E KA +I H N+    A L++  Q +G  Y R  T D +  RL  CVR + GHFE
Sbjct:  124 FVDIGVKINKDIYLKSILDDVLKPWSTSHFGSRQWCFQQDSAPAHKANVVQEWCRREIPDFIACGDWPSNSPNLNPLDFSVWSVVESKACSIRHQNLEELKAALTKAWQEMGSDYLR-MTCDAFMKRLRRCVREKGGHFE 262          

HSP 2 Score: 64.6994 bits (156), Expect = 1.009e-31
Identity = 32/74 (43.24%), Postives = 43/74 (58.11%), Query Frame = 2
Query: 1085 KCKQLLSRYDLEAFKNILFSDENIFTVEAKCNCQNM*LLAPSVQQIPPGKGYRERTQHPASVMVWAGVSHQGKT 1306
            +CK+LL R+   + +NI+FSDE IFTV    N QN   LA  ++Q         +T HP SVMVW  ++  GKT
Sbjct:   47 RCKKLLKRFAEGSHRNIVFSDEKIFTVAPVVNSQNNRALAKDIEQALSRGKIVGKTAHPTSVMVWGNITSDGKT 120          
BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Match: A0A0N4XXA5 (DDE_3 domain-containing protein OS=Nippostrongylus brasiliensis OX=27835 GN=NBR_LOCUS7600 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.211e-30
Identity = 51/140 (36.43%), Postives = 69/140 (49.29%), Query Frame = 1
Query: 1315 FIPEGVKLNEEK*ISLVIEPYVKPLIDFMFAGNNLVFQQHSVLLHRAKSTQSWCPTNLPEFITTKNWPASRPDLNPLDYGI*SILEQKALAINHSNVANLSQFDQG----LGRYYTRNCTIDQWRNRLSACVRNRAGHFE 1722
            F+P+ VKLN +     ++E  + P     F      FQQ     HRA   Q WC T +P+ I +  WP   PDLNPLDY I S+LE KA +  H ++A+L Q  Q     +   Y R   +D +  RL AC+    G  E
Sbjct:  204 FVPQNVKLNAKIYKEDILEKVLLPWARNHFGSRQWTFQQDGAPAHRANVVQQWCRTQIPDIIASTEWPPYSPDLNPLDYSIWSVLESKACSTAHRSIASLEQSLQKAWSEMNVEYLR-AAVDAFPRRLRACIAANGGLIE 342          

HSP 2 Score: 65.855 bits (159), Expect = 3.211e-30
Identity = 31/75 (41.33%), Postives = 45/75 (60.00%), Query Frame = 2
Query: 1085 KCKQLLSRYDLEAFKNILFSDENIFTVEAKCNCQNM*LLAPSVQQIPPGKGYRERTQHPASVMVWAGVSHQGKTP 1309
            +CK+LL R+      NI+FSDE +FT+E   N QN  ++  SV++         RT +PA VMVWA ++  GK+P
Sbjct:  127 RCKRLLRRFSAARHLNIVFSDEKLFTIEQSLNRQNNRIIVSSVREANSRGRIVGRTSNPARVMVWASITSDGKSP 201          

HSP 3 Score: 25.7942 bits (55), Expect = 3.211e-30
Identity = 11/27 (40.74%), Postives = 17/27 (62.96%), Query Frame = 3
Query:  987 LSMRRMVKIDLNLKSLKMRKTHMLNHH 1067
            +S  R+V+ DLNL+S + +K H L   
Sbjct:   93 MSAWRLVRKDLNLRSYRCQKAHPLTPE 119          
BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Match: A0A016S9F8 (DDE_3 domain-containing protein OS=Ancylostoma ceylanicum OX=53326 GN=Acey_s0265.g654 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.686e-29
Identity = 53/141 (37.59%), Postives = 78/141 (55.32%), Query Frame = 1
Query: 1315 FIPEGVKLNEEK*ISLVIEPYVKPLIDFMFAGNNLVFQQHSVLLHRAKSTQSWCPTNLPEFITTKNWPASRPDLNPLDYGI*SILEQKALAINHSNVANLSQFDQG----LGRYYTRNCTIDQWRNRLSACVRNRAGHFEI 1725
            F+  G K+N E  +  +++  V P     F      FQQ S   H+AK  Q WC   LP+FI+ ++WP   PDLNP+DY + SIL+ KA A +H+ + +L +  Q     L   Y R+ T+D +  RL AC++ + G FEI
Sbjct:  124 FVDIGTKVNTEYHLEHILKKEVLPWACSHFGSEPWTFQQDSAPAHKAKVVQKWCANTLPDFISAEDWPPYSPDLNPMDYSVWSILKAKACAKSHTCLKSLREALQKAWDELDDDYLRS-TVDAFPRRLQACIKAKGGLFEI 263          

HSP 2 Score: 62.3882 bits (150), Expect = 1.686e-29
Identity = 31/75 (41.33%), Postives = 45/75 (60.00%), Query Frame = 2
Query: 1085 KCKQLLSRYDLEAFKNILFSDENIFTVEAKCNCQNM*LLAPSVQQIPPGKGYRERTQHPASVMVWAGVSHQGKTP 1309
            +C+  L R+     +NILF+DE+IFTVE   N QN   +A S++ +        R+ HP SVMV+ G++  GKTP
Sbjct:   47 RCRAFLKRFSEARHRNILFTDESIFTVEQFVNHQNDRAIAESLEALNITARTSLRSGHPKSVMVFGGITANGKTP 121          
BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Nematostella
Match: EDO46746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNW4])

HSP 1 Score: 45.8246 bits (107), Expect = 2.297e-6
Identity = 25/81 (30.86%), Postives = 43/81 (53.09%), Query Frame = 2
Query:  236 DVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISE---EVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIE 469
            D ++ LVD Q   GSW   +++   + V+     N  P  I +   ++W TV +  +L  K  +RE EW ++A K+K W++
Sbjct:    8 DKLMTLVDLQKACGSWELTDALAACLNVSKDVLVNAKPQSIPDLGSDIWATVLVLVWLSGKLFNREDEWEMIANKSKCWLK 88          
BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Nematostella
Match: EDO46747 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNW5])

HSP 1 Score: 49.2914 bits (116), Expect = 4.413e-6
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 2
Query:  236 DVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISE---EVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSI 478
            D ++ LVD Q   GSW   +++   + V+     N  P  I +   ++W TV +  +L  K  +R+ EW ++A K+K+W++ S+
Sbjct:  551 DKLMTLVDLQKACGSWELTDALAACLNVSKDVLVNAKPQSIPDLGSDIWATVLVLVWLSGKLFNRKDEWEMIANKSKHWLKSSL 634          
BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Match: SMESG000053671.1 (SMESG000053671.1)

HSP 1 Score: 305.449 bits (781), Expect = 1.295e-92
Identity = 175/177 (98.87%), Postives = 176/177 (99.44%), Query Frame = 2
Query:    2 MKAGAPQIVYDRYEPNPPPMKCPEAXXXXXXXXXXXXEDIGYLDKIKNMFYFGKKASKQNRKSKDXXXXXXXXXXXXXDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            MKAGAPQIVYDRYEPNPPPMKCPEAKFKKSKRTSLKKEDIGYLDKIKNMFYFGKKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQ+ VLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ
Sbjct:  621 MKAGAPQIVYDRYEPNPPPMKCPEAKFKKSKRTSLKKEDIGYLDKIKNMFYFGKKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQYFVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 797          
BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Match: SMESG000053671.1 (SMESG000053671.1)

HSP 1 Score: 296.59 bits (758), Expect = 1.950e-89
Identity = 161/177 (90.96%), Postives = 162/177 (91.53%), Query Frame = 2
Query:    2 MKAGAPQIVYDRYEPNPPPMKCPEAXXXXXXXXXXXXEDIGYLDKIKNMFYFGKKASKQNRKSKDXXXXXXXXXXXXXDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            MKAGAPQIVYDRYEPNPPPMKCPE              DIGYLDKIKNMFYFGKKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQ+ VLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ
Sbjct:  621 MKAGAPQIVYDRYEPNPPPMKCPE--------------DIGYLDKIKNMFYFGKKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQYFVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 783          
BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Match: SMESG000053667.1 (SMESG000053667.1)

HSP 1 Score: 213.001 bits (541), Expect = 1.633e-65
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 2
Query:  128 LDKIKNMFYFGKKASKQNRKSKDXXXXXXXXXXXXXDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            L K K      +KASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKES+REKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLL 
Sbjct:    3 LKKSKRTSLKKEKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESNREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLH 137          
BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Match: SMESG000053667.1 (SMESG000053667.1)

HSP 1 Score: 209.92 bits (533), Expect = 4.595e-64
Identity = 121/127 (95.28%), Postives = 122/127 (96.06%), Query Frame = 2
Query:  152 YFGKKASKQNRKSKDXXXXXXXXXXXXXDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            Y    ASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKES+REKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLL 
Sbjct:   29 YQSFSASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESNREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLH 155          
BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Match: SMESG000053638.1 (SMESG000053638.1)

HSP 1 Score: 100.138 bits (248), Expect = 8.640e-22
Identity = 43/99 (43.43%), Postives = 70/99 (70.71%), Query Frame = 2
Query:  236 DVIIYLVDKQNINGSWNFNESILLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKKAKNWIEKSIAESDLQDLAFREASDLLQ 532
            D+I++LVD QNINGSWN++E I   IG+++   +   P +I++ +W T  IYQ L++KE +REKEWRL A+KAK WIE+++ +S + +    ++++L  
Sbjct:  472 DIIMFLVDLQNINGSWNYDEKIFAYIGIDVNRIKEIQPDKIADIIWSTAVIYQHLMIKEQAREKEWRLFARKAKEWIEQNLNDSAIFNDILNKSAELFH 570          
The following BLAST results are available for this feature:
BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch73-265h17.45.411e-628.57si:ch73-265h17.4 [Source:ZFIN;Acc:ZDB-GENE-131121-... [more]
si:ch73-265h17.56.858e-628.57si:ch73-265h17.5 [Source:ZFIN;Acc:ZDB-GENE-131121-... [more]
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZID15.689e-5797.58Vault protein inter alpha trypsin-1 (Fragment) OS=... [more]
A0A368FVD92.900e-3237.14Uncharacterized protein OS=Ancylostoma caninum OX=... [more]
A0A368H7I11.009e-3137.14Uncharacterized protein OS=Ancylostoma caninum OX=... [more]
A0A0N4XXA53.211e-3036.43DDE_3 domain-containing protein OS=Nippostrongylus... [more]
A0A016S9F81.686e-2937.59DDE_3 domain-containing protein OS=Ancylostoma cey... [more]
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO467462.297e-630.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO467474.413e-629.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of vault protein inter alpha trypsin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of vault protein inter alpha trypsin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000053671.11.295e-9298.87SMESG000053671.1[more]
SMESG000053671.11.950e-8990.96SMESG000053671.1[more]
SMESG000053667.11.633e-6591.85SMESG000053667.1[more]
SMESG000053667.14.595e-6495.28SMESG000053667.1[more]
SMESG000053638.18.640e-2243.43SMESG000053638.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30026256
GenBankAFJ24785.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026256 ID=SMED30026256|Name=vault protein inter alpha trypsin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=2091bp
AATGAAGGCGGGAGCTCCACAGATAGTGTACGACAGGTATGAGCCGAATC
CTCCACCAATGAAATGTCCTGAAGCGAAATTTAAGAAATCTAAAAGAACT
TCTTTGAAAAAAGAGGATATTGGCTATTTGGATAAGATCAAGAATATGTT
CTACTTTGGAAAAAAGGCTTCTAAACAAAATCGAAAATCAAAGGATTCCT
CATCTGAAGAAGCTGTTTCGGAACCTGAACCGGTAGATGTTATCATTTAT
CTTGTAGATAAACAAAACATAAATGGCTCTTGGAATTTCAACGAATCAAT
CTTATTGAAAATAGGCGTCAATATTACAGAATTTAGAAATAAAATGCCCG
GAAGGATATCTGAAGAAGTCTGGATTACTGTTGCCATTTATCAATTCCTC
GTTCTGAAGGAGAGTAGTAGGGAAAAAGAATGGCGTCTTGTTGCAAAGAA
AGCGAAAAATTGGATTGAAAAGAGCATAGCCGAGAGCGATTTACAAGATT
TGGCTTTTAGAGAAGCTTCTGATCTACTTCAGTGAATTCTTAATTATTTA
GTTTTGACTTTTGGACTTATTGCCTTTGACAGTTATTTGATTGATATATG
ATTTTGATTACTCAAGAAATAAATTGTTTTGTTCTTTGCAAATATGTAAC
CAATGTTCCATGGGAGATATTCCCACTTGAAACTGCTTTATAGTGCAGAC
AGTATCGTTATTTTCTCACATTGCTTTACCAACAATGAAGTCATTTGCAT
TTTGCTCTGCTCTGATATTATCGCTTATGCTACAATATTTATCTCCATCT
CAAGTTGTTCAGAAGGGAATGGTCCATGGATATTAAATGAGCCACAGCTT
ATCGACACATAAAAACGTTAATCGCTTCTGGCTGCATTACTGCCCAGCGT
TGCACGGCACATATCGCAGGGTCTGCACACCAAAGCTAATTAAAGAGATC
TCTAATAAAGTTGGCTTGTGATTTGAAAGTGTCAAGCTGTCAATGCGTAG
AATGGTCAAAATTGATCTTAATCTCAAGTCTCTTAAAATGAGAAAAACCC
ATATGCTCAACCATCATTCAATCAGCACGGTTAAAAATGCAAACAGTTAT
TATCAAGGTACGACCTGGAGGCTTTCAAAAATATCCTCTTCTCAGATGAA
AATATTTTCACTGTAGAAGCTAAATGTAACTGTCAGAATATGTGATTGCT
TGCCCCTAGTGTCCAGCAAATCCCTCCAGGGAAAGGTTATCGAGAAAGAA
CTCAGCATCCAGCATCTGTTATGGTTTGGGCAGGAGTGTCCCATCAAGGC
AAGACTCCTTTGGTTTTATCCCTGAAGGTGTCAAGCTCAATGAAGAAAAA
TAAATTTCTCTGGTTATCGAGCCGTATGTGAAGCCTCTTATTGATTTCAT
GTTTGCTGGTAACAACTTGGTTTTCCAGCAACACTCAGTACTCTTACATC
GAGCAAAAAGCACACAATCCTGGTGTCCGACAAATTTACCCGAATTTATC
ACAACGAAGAACTGGCCAGCTTCAAGACCAGACCTGAACCCCCTCGACTA
TGGAATTTGATCCATATTAGAGCAGAAGGCTCTTGCGATTAATCATAGCA
ATGTCGCTAACCTCAGTCAATTTGATCAAGGCTTGGGAAGATATTACACA
AGAAACTGCACGATTGATCAGTGGCGAAATAGACTTTCAGCTTGTGTGAG
AAATAGAGCTGGACATTTTGAAATCTGAACAATGATTATTATACTCTGAC
ATGTTGCACATTTTCCCATATTCAGTAGACTAATTTGAATAATAACATTA
GGTATTATGGCTGTTTTCATTTTGTCTCATTTTGTTTGGGTCACCCTGTA
TATGGACATTAACAGTAATTAGTGAGAATTTATAAACATACTCTAAAAAA
TAAAATTAAAATCTTCAAATTTAAATTAATTTGATTTAAACTTTGTCTAA
AAAGGGCTTCTCAAACCATACGTCAAAAAATGGGTTATTATTATAAATAA
TTAGTTTACTGGAGTTATTTATATACTTGCAGCGTTTTCCAAACTTTAAA
AAATTTCATGAATGGCGGTGCCTATAATATTTTAAGATGGC
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protein sequence of SMED30026256-orf-1

>SMED30026256-orf-1 ID=SMED30026256-orf-1|Name=SMED30026256-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=178bp
MKAGAPQIVYDRYEPNPPPMKCPEAKFKKSKRTSLKKEDIGYLDKIKNMF
YFGKKASKQNRKSKDSSSEEAVSEPEPVDVIIYLVDKQNINGSWNFNESI
LLKIGVNITEFRNKMPGRISEEVWITVAIYQFLVLKESSREKEWRLVAKK
AKNWIEKSIAESDLQDLAFREASDLLQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27