MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30026153
Length (bp)1560
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MFS domain-containing protein (SMED30026153) t-SNE clustered cells

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30026153) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MFS domain-containing protein (SMED30026153) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30026153) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026153

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30026153h1SMcG0009008 Contig3964newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0009008 Contig3964uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0016135 Contig43770uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0016135 Contig43770newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0016135 Contig3964newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0016135 Contig3964uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30026153h1SMcG0016135 dd_Smed_v4_7075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30026153h1SMcG0016135 dd_Smed_v4_7075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0022818 Contig3964newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026153h1SMcG0022818 Contig3964uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of MFS domain-containing protein vs. Ensembl Human
Match: SLC16A12 (solute carrier family 16 member 12 [Source:HGNC Symbol;Acc:HGNC:23094])

HSP 1 Score: 53.1434 bits (126), Expect = 7.797e-7
Identity = 50/229 (21.83%), Postives = 96/229 (41.92%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNI--DELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSI 725
            M  +    S  P      + ++ G FL+ +   +    ++I   E    + Q+++         ++ WI+SI+         LG  + N +      ++GG L S    L+  +  T    + L+ G++ GLG  + Y  ++A   KYF + + L +GI +SG GI   I   +   L+   + +       G+ LILG     +   G+L+  +   ++  T   N +
Sbjct:   31 MAKVNRARSTSPPDGGWGWMIVAGCFLVTICTRAVTRCISIFFVEFQTYFTQDYA---------QTAWIHSIVDCVTMLCAPLGSVVSNHLSCQVGIMLGGLLASTG--LILSSFATSLKHLYLTLGVLTGLGFALCYSPAIAMVGKYFSRRKALAYGIAMSGSGIGTFILAPVVQLLIEQFSWR-------GALLILGGFVLNLCVCGALMRPITLKEDHTTPEQNHV 241
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 127.872 bits (320), Expect = 9.327e-32
Identity = 113/420 (26.90%), Postives = 205/420 (48.81%), Query Frame = 3 
Query:   90 NRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIM---VFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVN----------NKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSI----------SAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIY---GTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSF 1271
            NR   +L GA  I L++G++++F N+   +  YM+N  ++   V I    WI +      F M     +GG   +      +A IG +LV+ ++ L    ++  FA+  + YGL+ GLG GIAY  +++  + + P   G+I GI  +GFG+S++IF  IQ  ++  +N            +++ ++P  F  L +IY  +QA+   LI V +    +++S  SI            +  +  N++  ++ +   +++    F+ L+  +F  SF+   F  +YK Y +  S ++ D+  ++     ++SV N   RI WG LTD+  F+  I + T +C      L++ ++     M +++   GTF+ +     + ++LF  T   K FG  +   NYG L  S +TS I    +S F
Sbjct:   22 NRICVILFGAVSIHLTIGTYHTFGNMLPYMASYMRN--FTDPSVRIEHMMWIPTFQGCFPFAM----VIGGLTSSTFSPRVSAFIGCFLVTSSVALSAYAIQHSFALFFIVYGLLFGLGSGIAYVTAVSTAINWAPDKIGVISGIVAAGFGLSSSIFAPIQTMIVNPLNLPATKDGYFLQPELLHRVPALFTKLAVIYGIMQAMA--LIVVCDPPFRKSRSTESILEEDDEDMDEDYERPVFDNDEDTTIQMTSSEMLKSPTFYCLFASLFCCSFYANMFYNLYKTYGE--SFIEDDM--FMAMAFSIASVANAIARIGWGYLTDRTSFQ--IALSTATCLASVFLLTMPMTRELGKMAFLFWLVGTFICM----GATHALFI-TATVKCFGNRHKANNYGYLILSTTTSGILLAAISQF 422
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.457 bits (267), Expect = 8.970e-25
Identity = 103/421 (24.47%), Postives = 195/421 (46.32%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ----DKLMRNVNNK------KIMEKLPGSFLILGIIYAFIQAIGSLLINVKNND-EVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFIS--SHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSS 1268
            ME    ++ ++P    R + VL+G  +  ++ G  Y+  N+   +V Y +   Y   +    +  W+ +++  G+     +GG +E   G  + AL+G  + +  +   F +++  +A   L+ G +A +G  IAY   L    ++FP N GL  GI + G+G  A I   +Q    + L   VN++       ++E++P  F+++ I +   Q+IG + I     D  VET        ++ I TN + +         +    F +L++ +  N+ W+   S +YK Y +    +D D   +L  I  +SS+FN   R+FWG + D   ++  + I+    C L   L+  +  +   S+ +    T  ++ + +    S+ FP +  K F   NF   YG L  + S + + +GL+S+
Sbjct:   15 METDDPILKRLPR-GARPWAVLVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTG--QLIWLQTLLS-GLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVNDQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVET--------ENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQF--IDSDF--FLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIV----CSLGAVLAFSMPLVKMVSNDVLFLATVCLMFSCIGGTFSI-FPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 414
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.071 bits (266), Expect = 9.689e-25
Identity = 104/420 (24.76%), Postives = 197/420 (46.90%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ----DKLMRNVNNK------KIMEKLPGSFLILGIIYAFIQAIGSLLINVKNND-EVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFK-KVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSS 1268
            ME    ++ ++P    R + VL+G  +  ++ G  Y+  N+   +V Y +   Y   +    +  W+ +++  G+     +GG +E   G  + AL+G  + +  +   F +++  +A   L+ G +A +G  IAY   L    ++FP N GL  GI + G+G  A I   +Q    + L   VN++       ++E++P  F+++ I +   Q+IG + I     D  VET        ++ I TN + +         +    F +L++ +  N+ W+   S +YK Y +    +D D   +L  I  +SS+FN   R+FWG + D   ++  + ++ +L   L   A S+ L  + S+ +    T  ++ + +    S+ FP +  K F   NF   YG L  + S + + +GL+S+
Sbjct:    1 METDDPILKRLPR-GARPWAVLVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTG--QLIWLQTLLS-GLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVNDQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVET--------ENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQF--IDSDF--FLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVL---AFSMPLVKMVSNDVLFLATVCLMFSCIGGTFSI-FPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 400
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693542:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.071 bits (266), Expect = 9.689e-25
Identity = 104/420 (24.76%), Postives = 197/420 (46.90%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ----DKLMRNVNNK------KIMEKLPGSFLILGIIYAFIQAIGSLLINVKNND-EVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFK-KVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSS 1268
            ME    ++ ++P    R + VL+G  +  ++ G  Y+  N+   +V Y +   Y   +    +  W+ +++  G+     +GG +E   G  + AL+G  + +  +   F +++  +A   L+ G +A +G  IAY   L    ++FP N GL  GI + G+G  A I   +Q    + L   VN++       ++E++P  F+++ I +   Q+IG + I     D  VET        ++ I TN + +         +    F +L++ +  N+ W+   S +YK Y +    +D D   +L  I  +SS+FN   R+FWG + D   ++  + ++ +L   L   A S+ L  + S+ +    T  ++ + +    S+ FP +  K F   NF   YG L  + S + + +GL+S+
Sbjct:    1 METDDPILKRLPR-GARPWAVLVGGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRTG--QLIWLQTLLS-GLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVNDQGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPEGDIHVET--------ENLIGTNSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQF--IDSDF--FLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVL---AFSMPLVKMVSNDVLFLATVCLMFSCIGGTFSI-FPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 400
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Match: slc16a12b (solute carrier family 16 member 12b [Source:NCBI gene;Acc:449947])

HSP 1 Score: 50.447 bits (119), Expect = 3.971e-6
Identity = 75/321 (23.36%), Postives = 131/321 (40.81%), Query Frame = 3 
Query:  240 WIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAI-------GSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWIL----WILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITL-------SCCLLYGALSICLSFISSHMIYIY--GTFLILINLVNS 1142
            WI+S++         LG  I N +      ++GG+L S    L+  +  T    +  + GL+ GLG  + Y  ++A    YF + + L +GI +SG GI   I   +   L+ + + +  +  L G  L L +  A ++ I         L ++ +    V+  + N    +S     +Q     ++ +  +L  DF +L     +L    S    Y  P    Y   + G+    + +L  I     V +  G I +G LTD+   KK   I  +        CCL    L   +  +   ++Y Y  G ++ LI +V S
Sbjct:   70 WIHSLVDCTTMLCAPLGSLIGNQLSCRIAVILGGFLASVG--LVLSSFATSLEYLYATLGLLTGLGFALCYTPAIAMVGIYFCERKALAYGIAMSGSGIGTFILAPVVQLLIEHYSWRGALLILGGFVLNLCVCGALLRPIILKEEEACPLPVDSECGYSVKPPTLNGGPTRSAASDAKQRCFQSMQEYHFLLMPDFLVLAGSFLLLASGCSLPFVYLVP----YALDV-GVGHQHAAFLMSI---LGVIDIVGNITFGWLTDRRCLKKYRNICYMFAVGMEGLCCLFIPLLRTFVWLVPFSVLYGYFDGAYVALIPVVTS 380
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Match: slc16a12 (solute carrier family 16 member 12 [Source:NCBI gene;Acc:394932])

HSP 1 Score: 55.0694 bits (131), Expect = 1.219e-7
Identity = 55/238 (23.11%), Postives = 105/238 (44.12%), Query Frame = 3 
Query:   66 ISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLE 779
            + K P      + +++G F + +   +    ++I      +++  SY  F     ++ WI+SI+         +G ++ N        ++GG L S    L+  +  T    + L+ G++ GLG  + Y  ++A   KYF K + L +GI +SG GI   I   +   L+   + +       G+ LILG   + +   G+L+  +   ++    S +    Q  +E NE T+S  L+
Sbjct:    8 LHKAPPDGGWGWMIVIGCFFVTVCTRAVTRCISIF-----FVEFQSY--FAQDYARTAWIHSIVDCSTMLCAPIGSYVSNHFSCQVGIILGGVLASTG--LVLSSFATSLEYLYLTLGVLTGLGFALCYSPAIAMVGKYFEKRKALAYGIAMSGSGIGTFILAPVVQLLIEQFSWR-------GALLILGGFVSNLCVCGALMRPITLKED----SLHYPLKQEQME-NEPTQSGRLD 224
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Match: atf6b (activating transcription factor 6 beta [Source:Xenbase;Acc:XB-GENE-1013925])

HSP 1 Score: 53.5286 bits (127), Expect = 6.921e-7
Identity = 57/236 (24.15%), Postives = 101/236 (42.80%), Query Frame = 3 
Query:   99 YFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLG---GFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEV-----ETKSG-----NSISAQSWIETNEQTKS 767
            + V+L +FL+ L        V   ++ +  +       FK +   + WI S+    MG +  +G   G   N  G   TA+IGG+L S    L       +F  +  S+G+ AG+G G+ Y  ++     YF K R L  G++ +G G    +   +   L R +  +  M       L L +  A ++ +     ++K+++E      + K G     N+I A S +   E+ K+
Sbjct:   35 WMVVLSSFLVHL-------LVMGSQMALGILNLEWLEEFKQSRGLTAWISSL---SMGITLIVGPIIGLFINTCGCRKTAMIGGFLTSLGWVLSAYAPNVHF--LFFSFGVTAGIGSGMVYLPAVVTVGHYFQKRRALAQGLSTTGTGFGTFLITVLLKYLCRELGWRNAMIIQGAVTLNLCVCGALMRPLDHDEEHLKDHEEAAVDPKDAKEGALLNVNAIKANSELHEEEEGKA 258
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Match: Slc16a12 (solute carrier family 16 (monocarboxylic acid transporters), member 12 [Source:MGI Symbol;Acc:MGI:2147716])

HSP 1 Score: 60.8474 bits (146), Expect = 2.223e-9
Identity = 86/393 (21.88%), Postives = 160/393 (40.71%), Query Frame = 3 
Query:   99 YFVLLGAFLIQLSLGSFYSFVNI--DELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLL--INVKNNDEVETKSGNSISAQSWIE--------TNEQTKSVDL----EPHKIILELDFWILWILMFINSFWIYYFSPM--YKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLF 1223
            + ++ G FL+ +   +    ++I   E    + Q++S         ++ WI+SI+         LG  + N +   +  ++GG L S     +  +  T    + LS G++ GLG  + Y  ++A   KYF + + L +GI +SG GI   I   +   L+   + +       G+ LILG     +   G+L+  I +K +  V  K+ N  S +   +        T E T++       + +  +L  DF +L + +    F  Y  SP+  Y +      G+    + +L  I     V +  G I +G LTD+   K    +  L    L G   +CL  + +  + +   F       +       P V A++ G  +     G+++
Sbjct:   19 WMIVAGCFLVTICTRAVTRCISIFFVEFQTYFAQDYS---------QTAWIHSIVDCMTMLCAPLGSVVSNQLSCQAGIMLGGLLASTG--FILGSFATSLKHLYLSLGVLTGLGFALCYSPAIAMVGKYFSRRKALAYGIAMSGSGIGTFILAPVVQLLIEQFSWR-------GALLILGGFVLNLCVCGALMRPITLKEDRSVPEKNHNRESQREDCKQASPYSPLTKECTETRLCCSLQQEYGFLLMSDFVVLAVSVL---FMAYGCSPLFVYLVPYALSVGVSHHQAAFLMSI---LGVIDIVGNITFGWLTDRRCLKNYRYVCYLFAVALDGLCYLCLPMLQTFPLLV--PFSCTFGYFDGAYVTLIPVVTAEIVGTTSLSSALGVVY 385
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Mouse
Match: Slc16a9 (solute carrier family 16 (monocarboxylic acid transporters), member 9 [Source:MGI Symbol;Acc:MGI:1914109])

HSP 1 Score: 51.9878 bits (123), Expect = 1.442e-6
Identity = 48/183 (26.23%), Postives = 84/183 (45.90%), Query Frame = 3 
Query:  231 KSPWIYSIMV-FGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNND-EVETKSGNSISAQSWIETNEQTKSVD 773
            K+ W+ S+    G+ AS     F+ +  G+    +  G+LV+  + L  +    YF  +  SYG++ GLG G+ Y  ++    +YF   RGL  G+  +G  +   I+ A+Q  L+           L G  LI+G +   I A GSL+  ++ +D     K+        +   NE+ K+ +
Sbjct:   50 KTAWVGSLASGVGLLASPVCSLFVSSF-GARPVTIFSGFLVAGGLMLSSLAPNIYF--LFFSYGIVVGLGCGLLYTATVTITCQYFDSRRGLALGLISTGSSVGLFIYAALQRMLIE-------FYGLDGCLLIVGALALNILACGSLMRPLQTSDCPFPEKTAPENVPDRYSMYNEKEKNPE 222
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|P37662|YHJX_ECOLI (Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83333 GN=yhjX PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 7.798e-19
Identity = 98/416 (23.56%), Postives = 179/416 (43.03%), Query Frame = 3 
Query:   78 PS-YKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRT-YFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVS--------SVFNFAGRIFWGILTDKILFKKVILI---ITL--SCCLLYGALSICLSFISSHMIYIYGTFLILINLVN---SGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSF 1271
            PS Y+  R+  L+G  + Q +LGS Y++                +     + +  + + ++  G+  S S+ G ++   G     +  G L+       F+T  +    ++ LS G++ GL  G  Y ++L+  VK+FP+ +GLI    +  +G+ +  F  I  +L+  V  +K       +F+I G I   +   G+ L+  K+  + E K+ N +  + +       K              +W+L ++     F     S +Y I      G+  D+++ L  + +VS        S+ N +GR+  GIL+DKI   +VI I   I+L     LL+  L+               TF   I  V     G    FP++ ++ FG+NN   NYG+++  F   +I   +++S 
Sbjct:    3 PSNYQRTRWLTLIGTIITQFALGSVYTW--------SLFNGALSAKLDAPVSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLG---FFLTAHSDNLMMLWLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLETVGLEK-------TFVIWGAIALLMIVFGATLM--KDAPKQEVKTSNGVVEKDYTLAESMRKP------------QYWMLAVM-----FLTACMSGLYVI------GVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFAPLNAV-------------TFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSICGSIIASL 362
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|Q51330|OXLT_OXAFO (Oxalate:formate antiporter OS=Oxalobacter formigenes OX=847 GN=oxlT PE=1 SV=3)

HSP 1 Score: 61.2326 bits (147), Expect = 1.113e-8
Identity = 61/288 (21.18%), Postives = 123/288 (42.71%), Query Frame = 3 
Query:  423 MAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISI-VSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLV---NSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFI 1274
            +AG G+GI YG+++    ++FP  RGL  G T +G+G+    F  +   +++                + G+  AF+     + I +     V    G   + +  + T++     D    +++    FW+LW      +F+   F  +  +      G    L+  +  I + + ++FN   R FWG ++DKI   K + ++       +G  ++ L+   +        F+ ++ +      G+   FP+  + +FG      NYG  + + +T++IF G L + I
Sbjct:  115 LAGAGVGIVYGIAMNTANRWFPDKRGLASGFTAAGYGLGVLPFLPLISSVLK----------------VEGVGAAFMYTGLIMGILIILIAFVIRFPGQQGAKKQIVVTDK-----DFNSGEMLRTPQFWVLW-----TAFFSVNFGGLLLVANSVPYGRSLGLAAGVLTIGVSIQNLFNGGCRPFWGFVSDKIGRYKTMSVV-------FGINAVVLALFPTIAALGDVAFIAMLAIAFFTWGGSYALFPSTNSDIFGTAYSARNYGFFWAAKATASIFGGGLGAAI 369
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8BGC3|MOT12_MOUSE (Monocarboxylate transporter 12 OS=Mus musculus OX=10090 GN=Slc16a12 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.555e-8
Identity = 86/393 (21.88%), Postives = 160/393 (40.71%), Query Frame = 3 
Query:   99 YFVLLGAFLIQLSLGSFYSFVNI--DELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLL--INVKNNDEVETKSGNSISAQSWIE--------TNEQTKSVDL----EPHKIILELDFWILWILMFINSFWIYYFSPM--YKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLF 1223
            + ++ G FL+ +   +    ++I   E    + Q++S         ++ WI+SI+         LG  + N +   +  ++GG L S     +  +  T    + LS G++ GLG  + Y  ++A   KYF + + L +GI +SG GI   I   +   L+   + +       G+ LILG     +   G+L+  I +K +  V  K+ N  S +   +        T E T++       + +  +L  DF +L + +    F  Y  SP+  Y +      G+    + +L  I     V +  G I +G LTD+   K    +  L    L G   +CL  + +  + +   F       +       P V A++ G  +     G+++
Sbjct:   19 WMIVAGCFLVTICTRAVTRCISIFFVEFQTYFAQDYS---------QTAWIHSIVDCMTMLCAPLGSVVSNQLSCQAGIMLGGLLASTG--FILGSFATSLKHLYLSLGVLTGLGFALCYSPAIAMVGKYFSRRKALAYGIAMSGSGIGTFILAPVVQLLIEQFSWR-------GALLILGGFVLNLCVCGALMRPITLKEDRSVPEKNHNRESQREDCKQASPYSPLTKECTETRLCCSLQQEYGFLLMSDFVVLAVSVL---FMAYGCSPLFVYLVPYALSVGVSHHQAAFLMSI---LGVIDIVGNITFGWLTDRRCLKNYRYVCYLFAVALDGLCYLCLPMLQTFPLLV--PFSCTFGYFDGAYVTLIPVVTAEIVGTTSLSSALGVVY 385
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|D4A734|MOT12_RAT (Monocarboxylate transporter 12 OS=Rattus norvegicus OX=10116 GN=Slc16a12 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.902e-8
Identity = 86/404 (21.29%), Postives = 160/404 (39.60%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNI--DELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAI-------GSLLINVKNNDEVETKSGNSISA--QSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLF 1223
            M  I  V S  P      + ++ G FL+ +   +    ++I   E    + Q++S         ++ WI+SI+         LG  + N +   +  ++GG L S    L+  +  T    + LS G++ GLG  + Y  ++A   KYF + +   +GI +SG GI   I   +   L+   + +  +  L G  L L +  A ++ I       G    + ++    + K  +  S   + W ET         + +  +L  DF +L + +    F  Y  SP++     +   +     +    +SI+  V +  G I +G LTD+   K    +  L    L G   +CL  + S  + +   F       +       P V A++ G  +     G+++
Sbjct:    1 MTKITRVGSASPPDGGWGWMIVAGCFLVTICTRAVTRCISIFFVEFQTYFAQDYS---------QTAWIHSIVDCMTMLCAPLGSVVSNQLSCQAGIMLGGLLASTG--LILGSFATSLKHLYLSLGVLTGLGFALCYSPAIAMVGKYFSRRKAFAYGIAMSGSGIGTFILAPVVQLLIEQFSWRGALLILGGFVLNLCVCGALMRPITLKEDPSGPEKSHDRDAQREDCKQASPYSPLTKEWTETRLCCSLQ--QGYGFLLMSDFVVLAVSVL---FMAYGCSPLFVYLVPYALSVGVSHHQAAFLMSIL-GVIDIVGNITFGWLTDRRCLKNYRYVCYLFAVALDGLCYLCLPMLQSFPLLV--PFSCTFGYFDGAYVTLIPVVTAEIVGTTSLSSALGVVY 385
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5ZJU0|MOT9_CHICK (Monocarboxylate transporter 9 OS=Gallus gallus OX=9031 GN=SLC16A9 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 5.390e-8
Identity = 43/156 (27.56%), Postives = 80/156 (51.28%), Query Frame = 3 
Query:  312 GSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNND--EVETKSGNSISAQSWIETNEQTKSVD 773
            G+   A+  G++V+  + +       YF  + LSYG++ GLG G+ Y  ++    +YF K RGL  G+  +G  +   I+ A+Q +L+        +  L G  LI+G +   I A GSL+  ++++D    E    + +  Q ++  +E+ K+V+
Sbjct:   77 GARPVAIFSGFMVAGGLMMSSFAPNIYF--LYLSYGIVVGLGCGLLYNATVTITCQYFDKRRGLALGLISTGSSVGLFIYAALQRELIE-------LYGLDGCLLIVGALSLNILACGSLMRPLESSDSPSPEKACTDKVPDQYFV-YHEKEKTVE 222
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 9.811e-67
Identity = 133/428 (31.07%), Postives = 232/428 (54.21%), Query Frame = 3 
Query:  105 VLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ------DKLMRNV---------NNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETK------SGNSISAQS------------WIETNEQTK---SVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
             ++G FLI  +LG+ Y+F N+   +V YM+ HS+S + +    + WI+ + +FG GAS + GG IE  IG+  TAL+G W++S  + L +  ++++  ++ ++YGL+ G+G GIAY V++AA + +FP  RGL+ GI V+GFG  + +FD  Q      D +  NV         +   I++++P  F+ LG+IYA +QA+G  L+      E+E +      +G+  +AQ              +ET+E       +D  P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:   10 AVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKW-IGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 425
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.148e-66
Identity = 133/428 (31.07%), Postives = 232/428 (54.21%), Query Frame = 3 
Query:  105 VLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ------DKLMRNV---------NNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETK------SGNSISAQS------------WIETNEQTK---SVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
             ++G FLI  +LG+ Y+F N+   +V YM+ HS+S + +    + WI+ + +FG GAS + GG IE  IG+  TAL+G W++S  + L +  ++++  ++ ++YGL+ G+G GIAY V++AA + +FP  RGL+ GI V+GFG  + +FD  Q      D +  NV         +   I++++P  F+ LG+IYA +QA+G  L+      E+E +      +G+  +AQ              +ET+E       +D  P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:   12 AVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKW-IGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 427
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HYG0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.076e-66
Identity = 132/428 (30.84%), Postives = 233/428 (54.44%), Query Frame = 3 
Query:  105 VLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ------DKLMRNV---------NNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETK------SGNSISAQ-SWIETNEQTKSVDLE--------------PHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
             ++G FLI  +LG+ Y+F N+   +V YM+ HS+S + +    + WI+ + +FG GAS + GG IE  IG+  TAL+G W++S  + L +  ++++  ++ ++YGL+ G+G GIAY V++AA + +FP  RGL+ GI V+GFG  + +FD  Q      D +  NV         +   I++++P  F+ LG+IYA +QA+G  L+      E+E +      +G+  +AQ + ++    +K V +E              P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:   10 AVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKW-IGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 425
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.101e-64
Identity = 131/422 (31.04%), Postives = 230/422 (54.50%), Query Frame = 3 
Query:  105 VLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ------DKLMRNV---------NNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETK------SGNSISAQ---------SWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
             ++G FLI  +LG+ Y+F N+   +V YM+ HS+S + +    + WI+ + +FG GAS + GG IE  IG+  TAL+G W++S  + L +  ++++  ++ ++YGL+ G+G GIAY V++AA + +FP  RGL+ GI V+GFG  + +FD  Q      D +  NV         +   I++++P  F+ LG+IYA +QA+G  L+      E+E +      +G+  +AQ         S     +  + +D  P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:   10 AVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKW-IGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPRAWKPRRRMDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 419

HSP 2 Score: 94.7449 bits (234), Expect = 1.114e-16
Identity = 61/234 (26.07%), Postives = 117/234 (50.00%), Query Frame = 3 
Query:  627 ILGIIYAFIQAIGSLLINVK-NNDEVETKSGNSISAQ-SWIETNEQTKSVDLE--------------PHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
            +LG  ++ + A+ +LL  V     E+  ++G+  +AQ + ++    +K V +E              P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:  429 LLGAAFSTLGAVFTLLFEVAGRRQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 651
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267DK45 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g4 PE=4 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 5.581e-64
Identity = 132/442 (29.86%), Postives = 233/442 (52.71%), Query Frame = 3 
Query:  105 VLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQ------DKLMRNV---------NNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVET--------------------KSGNSISAQ-SWIETNEQTKSVDLE--------------PHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSE-YLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFIN 1277
             ++G FLI  +LG+ Y+F N+   +V YM+ HS+S + +    + WI+ + +FG GAS + GG IE  IG+  TAL+G W++S  + L +  ++++  ++ ++YGL+ G+G GIAY V++AA + +FP  RGL+ GI V+GFG  + +FD  Q      D +  NV         +   I++++P  F+ LG+IYA +QA+G  L+      E+E                     ++G+  +AQ + ++    +K V +E              P +++ +  FW+LW    +NS  + Y +  YK +     GL   L++ YL  +  +++ FN A R+ WG L D++ ++ ++ +++    +L        +F  S   + Y  ++  +  + +GN   FP  CAKL+G  N   NYG+LFT+ +TS I   LL++ + 
Sbjct:    3 AVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKW-IGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEEVNRRKNNGYMYMPLRQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSF-----GLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMS------TFTLSGAPWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVLK 432
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000010927.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002229.1:6659:13971:1 gene:ENSAMXG00000010636.2 transcript:ENSAMXT00000010927.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.9214 bits (141), Expect = 6.492e-9
Identity = 73/299 (24.41%), Postives = 138/299 (46.15%), Query Frame = 3 
Query:  207 SGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFA----IVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLL----INVKNNDEVETKSGNSISAQSWIETNEQTKS---VDLEPHKIILE---LDFWILWILMFI--NSFWIY----YFSPMYKIYEKHLS-GLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKK--VILIITL 1034
            S F+ T  +  WI SI VF M  +  L   + N  G     + GG L+S        T+ T F      + ++ GL++GLG  + +  ++    +YF K R L+  +  +G   S          L  +V  +  +       +++G++ A + A G+LL    I  K +   E K G S+ ++S +++  Q+ S    +L+   ++ +   LDF +L    FI  + F ++    +F+P   I +  +S GL+ + + Y+     V+ +F   GR+  G + ++   +K  ++LI T+
Sbjct:   56 SHFEETNSRVSWIVSICVFVMTFTAPLSTVLSNRFGCRPVVMFGGLLISLG------TISTAFTHSINQMYITVGLVSGLGYCLTFLPTVTILSQYFNKRRSLVTSVASTGECFSLFALTPALSILKAHVGWRNCL-------VVIGLMQASLIAFGALLRPIIIQPKLSSPEEEKEGRSLPSKSSVDSGVQSISSSKTNLKEEPVVAQPKLLDFSVLRDCSFICYSLFGLFATLGFFAPQLYIIDLSVSCGLERNQATYMLSTMAVAEIF---GRLSIGWILNRRPVRKIYILLICTM 338
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Match: slc16a12b (solute carrier family 16 member 12 [Source:NCBI gene;Acc:111192338])

HSP 1 Score: 55.8398 bits (133), Expect = 6.527e-8
Identity = 49/191 (25.65%), Postives = 87/191 (45.55%), Query Frame = 3 
Query:  234 SPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDE------VETKSGNSISAQSWIETNEQTKSVDLEPHK 788
            + WI+S++         LG  I N +      ++GG+L S    L+  +  T    + LS G++ GLG  + Y  ++A    YF + + L +GI +SG GI   I   +   L+ + + +       G+ LILG + + +   G+LL  +   +E      V+++ G S      I   E+ K ++ E  K
Sbjct:   58 TAWIHSLVDCTTMLCAPLGSLIGNHLSCRVAVILGGFLASIG--LVLSSFATSLEYLYLSLGVLTGLGFALCYTPAIAMVGIYFCERKSLAYGIAMSGSGIGTFILAPVVQMLIEHYSWR-------GALLILGGLVSNLCVCGALLRPITLKEEEACPLPVDSECGYSTKPLVAISPAEE-KQLEAERSK 238
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Match: slc16a12b (solute carrier family 16 member 12 [Source:NCBI gene;Acc:111192338])

HSP 1 Score: 55.8398 bits (133), Expect = 7.145e-8
Identity = 49/191 (25.65%), Postives = 87/191 (45.55%), Query Frame = 3 
Query:  234 SPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDE------VETKSGNSISAQSWIETNEQTKSVDLEPHK 788
            + WI+S++         LG  I N +      ++GG+L S    L+  +  T    + LS G++ GLG  + Y  ++A    YF + + L +GI +SG GI   I   +   L+ + + +       G+ LILG + + +   G+LL  +   +E      V+++ G S      I   E+ K ++ E  K
Sbjct:   58 TAWIHSLVDCTTMLCAPLGSLIGNHLSCRVAVILGGFLASIG--LVLSSFATSLEYLYLSLGVLTGLGFALCYTPAIAMVGIYFCERKSLAYGIAMSGSGIGTFILAPVVQMLIEHYSWR-------GALLILGGLVSNLCVCGALLRPITLKEEEACPLPVDSECGYSTKPLVAISPAEE-KQLEAERSK 238
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Match: slc16a12b (solute carrier family 16 member 12 [Source:NCBI gene;Acc:111192338])

HSP 1 Score: 55.4546 bits (132), Expect = 9.905e-8
Identity = 49/191 (25.65%), Postives = 87/191 (45.55%), Query Frame = 3 
Query:  234 SPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDE------VETKSGNSISAQSWIETNEQTKSVDLEPHK 788
            + WI+S++         LG  I N +      ++GG+L S    L+  +  T    + LS G++ GLG  + Y  ++A    YF + + L +GI +SG GI   I   +   L+ + + +       G+ LILG + + +   G+LL  +   +E      V+++ G S      I   E+ K ++ E  K
Sbjct:   58 TAWIHSLVDCTTMLCAPLGSLIGNHLSCRVAVILGGFLASIG--LVLSSFATSLEYLYLSLGVLTGLGFALCYTPAIAMVGIYFCERKSLAYGIAMSGSGIGTFILAPVVQMLIEHYSWR-------GALLILGGLVSNLCVCGALLRPITLKEEEACPLPVDSECGYSTKPLVAISPAEE-KQLEAERSK 238
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Match: SLC16A12 (solute carrier family 16 member 12 [Source:HGNC Symbol;Acc:HGNC:23094])

HSP 1 Score: 50.8322 bits (120), Expect = 4.527e-7
Identity = 45/175 (25.71%), Postives = 73/175 (41.71%), Query Frame = 3 
Query:  192 QNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSG 716
            Q H    F      + WI SIM         +G  +    G  +T + GG + S     +  +       + L  GLMAGLG  + Y  S+A   +YF ++R L  G+ +SG G+   +   +  +L+  V   +      G+ L+LG   A +   G+LL  + +      K G
Sbjct:   47 QQH----FSKDTSTTAWINSIMDCTTMLFAPIGSLLSTRFGCRATVMFGGLVSSSG--FIIASFSPTIEFLYLFLGLMAGLGFALCYSPSIAIVSRYFQRHRLLATGLAMSGNGVGTFVLAPVVQQLLIEVYAWR------GALLLLGAFVAQLCVCGALLRPLASPATPTAKPG 209
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Medaka
Match: slc16a12a (monocarboxylate transporter 12-B-like [Source:NCBI gene;Acc:101175502])

HSP 1 Score: 55.0694 bits (131), Expect = 1.164e-7
Identity = 74/305 (24.26%), Postives = 136/305 (44.59%), Query Frame = 3 
Query:   99 YFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKK 1013
            + V+ G FL  + + +    V++    V++ Q H    F+     + WI+S++         LG F+ N +    T ++GG L S  + L        F  ++L  G++ GLG  +++  ++A   KYF + + L +GI +SG GI   +       L+ + + +       G+ LILG I + +   G+L+  +++ +  +TK   S   Q++     +     L P  +IL + F     L +  S  + Y  P    Y   + G+D   + +L     VS +    G I +G +TD+   KK
Sbjct:   25 WMVVAGCFLATICIRAVTRCVSM--FFVEF-QMH----FEKDYSTTAWIHSLIDSTTMLCAPLGSFLGNRLSCRVTVMLGGLLSSAGLVLSSFASSVEFLYISL--GVITGLGFALSFTPAIAIVGKYFVERKALAYGIALSGSGIGTFLLAPAVQLLIEHYSWR-------GALLILGGIVSNLCVCGALMRPLEDMEVQKTKP--SSRCQNFCLKTREYFGFILIPSFLILSVSF---LFLAYGCSSPVVYLVP----YALSV-GVDHQHAAFLMSTFGVSGI---VGNITFGWITDRKCLKK 300
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000069675.1 (SMESG000069675.1)

HSP 1 Score: 840.106 bits (2169), Expect = 0.000e+0
Identity = 439/448 (97.99%), Postives = 441/448 (98.44%), Query Frame = 3 
Query:   45 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQTFEIXXXXXXXXXXXXXXITLAYRGRSLMFYQS 1388
            MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSG KVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINV+NNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYK YE HLSG D DLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICL FISS MIYIYGTFL+LINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQTFEISCSVASAVSFLAALITLAYRGRSLMFYQS
Sbjct:    1 MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGLKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVENNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKKYENHLSGTDLDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLRFISSPMIYIYGTFLVLINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQTFEISCSVASAVSFLAALITLAYRGRSLMFYQS 448
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000069668.1 (SMESG000069668.1)

HSP 1 Score: 334.724 bits (857), Expect = 6.398e-110
Identity = 196/444 (44.14%), Postives = 278/444 (62.61%), Query Frame = 3 
Query:   87 KNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVD------------LEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQT----FEIXXXXXXXXXXXXXXITLAYRGRS 1370
            KN+ Y ++LGAF IQL+LG FY+  NI  +IV++MQN+S S   VT+  S WI+ +++ GMGA+ SLGGFIE  IG + T LIGGW++SFNICLMFVTM++Y +IV +SYGL+ G G+G+AYGVSL  P+KYFPK +GLIFGI VSG   S+ IFDA+QD ++     + IM +LP SFLILG IY+ IQ +GS+L+  +  D+ E +S  + S     E ++  + +D            L+ H ++  LDFWILW++MF+NSF +   S +  ++ ++ + L  + S  LK I  ++ +FN  GRIFWG   DK  FK ++LIITL+   + G L +  +   S  +  +  + I+I+   SG    FP VC KLFG+  FV NYG+LFTS S       L+   + P   +    F     +   +SFLAAL+TL YRG+S
Sbjct:    5 KNQNYLIVLGAFFIQLTLGGFYTSGNIQPIIVNFMQNYSSSQTSVTMENSVWIFGVLLLGMGAAISLGGFIEMQIGPTKTVLIGGWVMSFNICLMFVTMKSYLSIVIISYGLIGGFGMGVAYGVSLGTPLKYFPKCKGLIFGIVVSGISFSSIIFDAVQDSIIYK-GKEIIMNRLPMSFLILGFIYSVIQLVGSILMKKRTKDKKEERSSQNYSP----EASDGDQKLDFPKLKILNNPPELQTHHLLFVLDFWILWMIMFLNSFCV---SLITSLFNRYSAVLVNETSFNLKVIVTIAKIFNCFGRIFWGFAIDKQSFKMLMLIITLTISFMIGILPVISTLKVSWHLPTFAVWFIVIHFFLSGTFSIFPIVCCKLFGMKYFVCNYGLLFTSCSVGGTIISLVIMLVFPEFSSTPLVFRNMVIIGVILSFLAALLTLLYRGKS 440
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000069681.1 (SMESG000069681.1)

HSP 1 Score: 327.405 bits (838), Expect = 3.728e-107
Identity = 192/440 (43.64%), Postives = 284/440 (64.55%), Query Frame = 3 
Query:   87 KNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQS-----WIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHMIYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAI-FSGLLSSFINPG-GQTFEIXXXXXXXXXXXXXXITLAYRGRSLMFYQ 1385
            K R Y VL G++LIQLSLGS ++F +I   IV YM N+S+SG  V    +  I   + FG+GAS S+GG+ E  IG   T LIG WL+S NI LMF T+++Y ++V ++YG +AGLG+G AYGVSL  P+K+FP+++GL+ GI +SGFGIS+ IF+++QD  +   N    +  LP SFLIL  I+A +Q IGS+L+   ++D++E    NS+S +S      +       + ++EPH+IILE DFW+LWI+ F+NSF +Y+ S  Y +Y K  + +  ++   LK   I++S+ N AGRIF+G L D+  FK V+ IITLSC L  G L +  S  +++  YIYG  + +I     GN   FP VCA+LFG+ NFV+ YG+L+TS S S I  S L +SF++     +++I+  V  ++S +AA  T  +RG+SL+ ++
Sbjct:    7 KYRCYIVLFGSYLIQLSLGSLFTFGHIQPEIVKYMNNYSFSGSSVNSGNTVSIIGALTFGVGASLSIGGYAEQKIGPGKTILIGAWLMSLNIGLMFFTLKSYLSVVVITYGFIAGLGLGFAYGVSLGRPIKWFPEHKGLVIGIVISGFGISSAIFNSVQDAFLDEKNTN--INSLPKSFLILAGIFAVLQLIGSILVRQNSDDKLEETLINSVSVRSSKYKPILFDYSSIGNNEIEPHQIILERDFWVLWIVFFLNSFMVYFISASYALYFKTQTQIKNEI--ILKIYFIITSLCNGAGRIFFGYLIDRKPFKIVMYIITLSCTLFIGTLPLT-SLKNTYFPYIYGVSIGVIFFFTGGNYTVFPAVCAQLFGLKNFVFKYGILYTSCSVSGIVVSALSASFLSDATSHSYDITFIVCGSISLIAAGFTSFFRGKSLLIHR 441
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000069673.1 (SMESG000069673.1)

HSP 1 Score: 284.648 bits (727), Expect = 1.029e-90
Identity = 179/430 (41.63%), Postives = 253/430 (58.84%), Query Frame = 3 
Query:   96 RYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVKNNDEVETKSGNSISAQSW-IETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALS-ICLSFISSHMIYI-YGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQTFEIXXXXXXXXXXXXXXITLAYRGRSLM 1376
            +Y  + G FL+ +S+G FYS  +I   I  YM+N + +        S WI+   + GMGA+ SLGG++E  IGSS T LIGGW++S NICLM VTM     +V L+YGL AG G G+ YGV+L  P+K   K  GL  G+ VSGFG+SATIFD +Q+ L  +  N      LP SFLI+G IYA IQ IG++L+  K N+   T   N  S   + I  N ++K ++ +PH+I+LELDFWILWI  F+N+  + + + +Y  Y   L G     +++L  ISI  + FN  GRIFWG + DK  FK  ++I+T+   +L    S I L  ++  M  I +G     IN   SGN   FP +CA+L+G+  FV NYGMLFT+ S +     ++S  I+      +      +A+S L+ L+TL YRG+SL+
Sbjct:   10 KYLPIFGGFLLHISVGYFYSAGHIMPHINKYMKNENKT--------STWIFGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLKIVKKRVGLFLGVVVSGFGVSATIFDYLQNVLSSDYTN------LPLSFLIMGCIYAVIQLIGAILLKKKANENDLTIQSNETSFNYFEIFLNSKSK-MEFKPHEILLELDFWILWITFFLNAIGVNFLTTLYNEYYHELFG-----AQHLVTISISGAAFNCLGRIFWGFIIDKKPFKITMVIMTVGTSILMALFSTISLMNLTGKMNEIVFGIIFCAINFFQSGNFCIFPFMCAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMTLVYRGKSLI 419
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1)

HSP 1 Score: 272.707 bits (696), Expect = 2.741e-86
Identity = 176/446 (39.46%), Postives = 256/446 (57.40%), Query Frame = 3 
Query:   72 KIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQNHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGWLVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKNRGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIYAFIQAIGSLLINVK-NNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKIILELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIVSSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGAL-SICLSFISSHMI-YIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSAIFSGLLSSFINPGGQTFEIXXXXXXXXXXXXXXITLAYRGRSLMFYQS*LPI 1400
            K  SY   +Y  +LG+FL+ LS+G F+S   I   I +YM N++ +        S WI    + GMGA+ SLGG++E  IGSS T LIGGW++S NICLM VTM     +V L+YGL AG G G+ YGV+L  P++      GLI G+  SGFG+SA IF  +Q     N+      + LP SFLILG IYA IQ IG +LI  K N +E+  +S   +S            +++ +PH+I+LELDFWILWI++F+N+  I   + +Y  Y   L G     +++L  ISI  + FN  GRIFWG + DK  FK  ++I+T+   +L     +I L  ++  M  Y++G     IN   SGN   FP +CA+L+G+  FV NYGMLFT+ S +     ++ +  +   +  +      +A+S L+A++T+ YRG+SL+   + L I
Sbjct:    4 KFTSYG--KYLPILGSFLLHLSVGCFFSIGQIKPQIDEYMGNNNKT--------STWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQ-----NLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYD----------NLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFG-----AQHLVTISISGAAFNCLGRIFWGFIIDKKPFKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 419
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SLC16A127.797e-721.83solute carrier family 16 member 12 [Source:HGNC Sy... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Y59A8B.219.327e-3226.90pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
F10G7.58.970e-2524.47pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.59.689e-2524.76pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.59.689e-2524.76pep chromosome:WBcel235:II:4693542:4696408:1 gene:... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
slc16a12b3.971e-623.36solute carrier family 16 member 12b [Source:NCBI g... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc16a121.219e-723.11solute carrier family 16 member 12 [Source:NCBI ge... [more]
atf6b6.921e-724.15activating transcription factor 6 beta [Source:Xen... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Slc16a122.223e-921.88solute carrier family 16 (monocarboxylic acid tran... [more]
Slc16a91.442e-626.23solute carrier family 16 (monocarboxylic acid tran... [more]
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P37662|YHJX_ECOLI7.798e-1923.56Uncharacterized MFS-type transporter YhjX OS=Esche... [more]
sp|Q51330|OXLT_OXAFO1.113e-821.18Oxalate:formate antiporter OS=Oxalobacter formigen... [more]
sp|Q8BGC3|MOT12_MOUSE1.555e-821.88Monocarboxylate transporter 12 OS=Mus musculus OX=... [more]
sp|D4A734|MOT12_RAT1.902e-821.29Monocarboxylate transporter 12 OS=Rattus norvegicu... [more]
sp|Q5ZJU0|MOT9_CHICK5.390e-827.56Monocarboxylate transporter 9 OS=Gallus gallus OX=... [more]
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HL509.811e-6731.07MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267H5U21.148e-6631.07MFS domain-containing protein (Fragment) OS=Macros... [more]
A0A1I8HYG02.076e-6630.84MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8H2051.101e-6431.04MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267DK455.581e-6429.86MFS domain-containing protein (Fragment) OS=Macros... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSAMXT00000010927.26.492e-924.41pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
slc16a12b6.527e-825.65solute carrier family 16 member 12 [Source:NCBI ge... [more]
slc16a12b7.145e-825.65solute carrier family 16 member 12 [Source:NCBI ge... [more]
slc16a12b9.905e-825.65solute carrier family 16 member 12 [Source:NCBI ge... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SLC16A124.527e-725.71solute carrier family 16 member 12 [Source:HGNC Sy... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
slc16a12a1.164e-724.26monocarboxylate transporter 12-B-like [Source:NCBI... [more]
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069675.10.000e+097.99SMESG000069675.1[more]
SMESG000069668.16.398e-11044.14SMESG000069668.1[more]
SMESG000069681.13.728e-10743.64SMESG000069681.1[more]
SMESG000069673.11.029e-9041.63SMESG000069673.1[more]
SMESG000069670.12.741e-8639.46SMESG000069670.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026153 ID=SMED30026153|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1560bp
ACTAAATTTATCATATTTTTGAAAATACATAATTGAAATAATAAATGGAA
AATATTTATATTGTTATTTCAAAGATTCCAAGTTATAAAAACAGGCGATA
TTTCGTATTACTGGGAGCGTTTCTTATCCAACTTTCACTCGGAAGTTTTT
ACTCATTTGTGAATATTGACGAACTTATTGTTGATTACATGCAAAACCAC
AGCTATTCTGGATTTAAAGTTACTATATATAAATCTCCATGGATATATTC
TATAATGGTGTTTGGCATGGGAGCTTCATTTTCACTTGGCGGATTTATTG
AGAATTTGATAGGATCATCGAGCACAGCTTTAATTGGAGGATGGCTAGTA
AGTTTCAATATCTGTTTGATGTTTGTGACGATGAGGACATATTTTGCAAT
TGTCGCACTTTCATATGGACTAATGGCAGGCTTAGGAATTGGGATTGCTT
ACGGAGTGTCACTAGCTGCACCTGTCAAATATTTTCCAAAAAATAGAGGT
TTAATTTTTGGAATTACAGTTTCTGGATTTGGTATTTCGGCAACAATATT
TGATGCAATTCAGGATAAATTGATGCGAAATGTCAATAACAAGAAAATCA
TGGAGAAATTACCGGGCAGTTTTCTCATTCTAGGAATAATTTATGCTTTT
ATTCAAGCAATTGGATCTTTACTGATTAATGTGAAAAACAATGATGAGGT
TGAAACAAAAAGTGGAAATTCAATTTCAGCACAAAGTTGGATAGAGACAA
ATGAACAAACAAAATCCGTTGATTTGGAACCTCATAAAATTATATTGGAA
CTAGACTTTTGGATTCTTTGGATATTAATGTTTATCAATTCTTTCTGGAT
TTATTATTTTAGTCCAATGTATAAAATATACGAAAAACATCTCAGTGGCC
TGGATCCCGATTTGAGTGAATATTTAAAAAGAATATCAATCGTTTCTTCA
GTATTTAATTTTGCTGGGAGAATATTTTGGGGCATTTTAACCGATAAAAT
CTTATTTAAAAAAGTTATCCTTATAATTACTCTGTCATGCTGCCTACTTT
ATGGCGCCTTATCAATTTGTCTCAGTTTTATCTCTTCTCATATGATCTAT
ATATACGGGACCTTTCTAATCCTCATTAACTTGGTGAATTCTGGAAACTC
TTTATTCTTTCCAACAGTATGCGCGAAGTTATTTGGGATAAATAATTTTG
TGTATAATTATGGGATGTTGTTCACGTCATTTTCAACTTCGGCAATATTT
TCTGGATTATTAAGTAGTTTCATTAATCCCGGTGGACAAACGTTTGAAAT
ATCTTGTTCTGTAGCATCAGCAGTTTCATTTTTAGCTGCTTTGATAACGC
TGGCATACCGAGGCAGATCTCTTATGTTTTATCAATCTTGACTACCTATC
AACGCTACCCCCCCTAATCAGTCACTGGAAATTGATTTATTTGCACATTA
TTTCTGTCGATTTTATTATTCATTATTTTAACTAATAAATAATTTTCTCA
GATTCCAAATTACTTTTTTGATTTGCTTTTCAGATGACTTGATTAATTTA
TATTTTATAA
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protein sequence of SMED30026153-orf-1

>SMED30026153-orf-1 ID=SMED30026153-orf-1|Name=SMED30026153-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=449bp
MENIYIVISKIPSYKNRRYFVLLGAFLIQLSLGSFYSFVNIDELIVDYMQ
NHSYSGFKVTIYKSPWIYSIMVFGMGASFSLGGFIENLIGSSSTALIGGW
LVSFNICLMFVTMRTYFAIVALSYGLMAGLGIGIAYGVSLAAPVKYFPKN
RGLIFGITVSGFGISATIFDAIQDKLMRNVNNKKIMEKLPGSFLILGIIY
AFIQAIGSLLINVKNNDEVETKSGNSISAQSWIETNEQTKSVDLEPHKII
LELDFWILWILMFINSFWIYYFSPMYKIYEKHLSGLDPDLSEYLKRISIV
SSVFNFAGRIFWGILTDKILFKKVILIITLSCCLLYGALSICLSFISSHM
IYIYGTFLILINLVNSGNSLFFPTVCAKLFGINNFVYNYGMLFTSFSTSA
IFSGLLSSFINPGGQTFEISCSVASAVSFLAALITLAYRGRSLMFYQS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
GO:0008028monocarboxylic acid transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
GO:0015718monocarboxylic acid transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 22..391
e-value: 5.0E-16
score: 58.6
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 253..442
e-value: 1.6E-9
score: 39.4
coord: 12..222
e-value: 2.0E-7
score: 32.6
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 16..435
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 16..435
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 17..437
e-value: 2.46037E-53
score: 181.269
NoneNo IPR availableTMHMMTMhelixcoord: 153..172
NoneNo IPR availableTMHMMTMhelixcoord: 350..372
NoneNo IPR availableTMHMMTMhelixcoord: 19..41
NoneNo IPR availableTMHMMTMhelixcoord: 116..138
NoneNo IPR availableTMHMMTMhelixcoord: 255..272
NoneNo IPR availableTMHMMTMhelixcoord: 82..104
NoneNo IPR availableTMHMMTMhelixcoord: 416..438
NoneNo IPR availableTMHMMTMhelixcoord: 384..406
NoneNo IPR availableTMHMMTMhelixcoord: 189..211
NoneNo IPR availableTMHMMTMhelixcoord: 324..346
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 15..439