Nad dependent epimerase dehydratase

Overview
NameNad dependent epimerase dehydratase
Smed IDSMED30026136
Length (bp)993
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nad dependent epimerase dehydratase (SMED30026136) t-SNE clustered cells

Violin plots show distribution of expression levels for Nad dependent epimerase dehydratase (SMED30026136) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nad dependent epimerase dehydratase (SMED30026136) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nad dependent epimerase dehydratase (SMED30026136) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026136

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30026136h1SMcG0020061 Contig37373uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026136h1SMcG0020061 Contig37373newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30026136h1SMcG0020061 dd_Smed_v4_3452_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30026136h1SMcG0020061 dd_Smed_v4_3452_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026136h1SMcG0020061 dd_Smed_v4_3452_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Match: A0A5P8I4L8 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.513e-70
Identity = 113/221 (51.13%), Postives = 162/221 (73.30%), Query Frame = 1 
Query:  220 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPIL--VKKMPRFEELHIKMIRHAF-QPFKELE-NDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEF 870
            M +  +++LVIG GQMRTGT+SLK ALE+L+++ CYHM E++N+HKDHV LWI+  D+  ++++T+ +IF +Y AT D+P A  YKDL++IYPN KV+LTTRNS+DW+ SV++T + R +   SF+ H IL        F  +  KM   A+ +PF  L  +DE+LK+ YE +NE+VR  +PKERLLEF   +GW+PLCRFL++  +PNE +PFVN+TK+F
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTL-RNNMKTSFV-HKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNL-PVPNEPYPFVNTTKDF 218
[Close]
BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Match: A0A2H1CLP5 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_04199 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.241e-51
Identity = 94/232 (40.52%), Postives = 135/232 (58.19%), Query Frame = 1 
Query:  241 LLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETS------DRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMP-RFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS*RNYFLLT 915
            LLVIG G  RTGT SLK ALE L+ K CYHM EL++ H DHV+LW+   +    D E+        +IF  Y  TTDYP    YK L++IYP  K++LT R+   W+ SVR TI PR      ++   ++   +P +F ++    ++++  P  +L NDE + + +  +N++V+  +P +RLL F V DGWKPLC FL+ K IP+  FP VN   +   RF    R Y++ T
Sbjct:    9 LLVIGAGLGRTGTKSLKDALELLYQKPCYHMTELVHKHPDHVELWLRLFEQLKQDPESELSGQVLKKIFQGYSLTTDYPACSIYKQLMRIYPEAKIVLTVRDPSRWIESVRETIWPRESRLKKYVFDQVIKIVLPAKFSQMATNAVQYSMGPNVDLNNDEQMIQGFIRWNQEVKQVVPNDRLLIFDVKDGWKPLCTFLN-KPIPDHPFPHVNDRAQVKSRF----RQYYVKT 235
[Close]
BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Match: A0A4E0RFY7 (Cell wall integrity and stress response component 1 OS=Fasciola hepatica OX=6192 GN=D915_002646 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.203e-51
Identity = 96/241 (39.83%), Postives = 138/241 (57.26%), Query Frame = 1 
Query:  217 NMKNEN-HQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETS------DRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMP-RFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS*RNYFLLT 915
             M N+    LLVIG G  RTGT SLK ALE L+ K CYHM EL++ H DHV+LW+   +    D E+        +IF  Y  TTDYP    YK L++IYP  K++LT R+   W+ SVR TI PR      ++   ++   +P +F ++    ++++  P  +L NDE + + +  +N++V+  +P +RLL F V DGWKPLC FL+ K IP+  FP VN   +   RF    R Y++ T
Sbjct:   26 TMSNDGTATLLVIGAGLGRTGTKSLKDALELLYQKPCYHMTELVHKHPDHVELWLRLFEQLKQDPESELSGQVLKKIFQGYSLTTDYPACSIYKQLMRIYPEAKIVLTVRDPSRWIESVRETIWPRESRLKKYVFDQVIKIVLPAKFSQMATNAVQYSMGPNVDLNNDEQMIQGFIRWNQEVKQVVPNDRLLIFDVKDGWKPLCTFLN-KPIPDHPFPHVNDRAQVKSRF----RQYYVKT 261
[Close]
BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Match: A0A183AA27 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3812 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 3.429e-51
Identity = 97/241 (40.25%), Postives = 141/241 (58.51%), Query Frame = 1 
Query:  226 NENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETS------DRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPR--VHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS*RNYFLLTSPS 924
             E+  +LVIG G  RTGT SLK+ALE L++K CYHM E+ + H DHV LWI   D    D +T        RIF  Y  TTD+PG + YK L+++YP+ KVILT R+   W+ S+R T+ P+  +  +G   +   L+   P FE +  + +  A     +L +DE +K+A+   NE+V+  +P +RLL F+V +GW+PLCRFL  K IP   FP VN  +   ER ++  R   ++ + S
Sbjct:   11 QEDAPMLVIGAGVGRTGTLSLKIALEILYEKPCYHMTEIAHKHLDHVKLWIELHDRLVQDMDTELPQDVIRRIFKGYQLTTDHPGCVIYKQLMKMYPDAKVILTVRDPNTWIQSLRETVKPKRPLFGSGWLKTLKELIILTPGFEHMTDQSVSLALGKNLDLNDDEQMKQAFVRRNEEVKLVVPADRLLVFQVKEGWEPLCRFLG-KPIPEVPFPHVNDRQSMKERLKQFQRRANIVVASS 250
[Close]
BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Match: A0A183AA35 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3820 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.710e-48
Identity = 101/236 (42.80%), Postives = 130/236 (55.08%), Query Frame = 1 
Query:  223 KNENHQ-LLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDN--RSIDKETS----DRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKM-------PRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEK 888
            K + H  LLVIG G  RTGT +LK ALE L+ K CYHM E++  H DHV LW    D   + ID E       RIF  Y  TTD PG   YK L++IYP  KVILTTR+   W+ S+R T+ P+    G     P  + KM       P F  +  + +  +      L+NDE +KRA+   NE+V  ++P +RLL F+V DGW+PLCRFL  K IP+  FP VN  K    R  +
Sbjct:    9 KEDAHAPLLVIGAGVGRTGTMTLKTALEILYQKPCYHMTEIVYKHLDHVKLWTKLHDRIEQDIDAELPADMIKRIFKGYQMTTDNPGCTIYKQLMKIYPEAKVILTTRDPNTWIQSIRETVRPKQPLFG-----PGWLDKMKERLVMTPGFSHMADQSLTLSLGSNVNLDNDEQMKRAFVRRNEEVERTVPADRLLVFQVKDGWEPLCRFLG-KPIPDVPFPHVNDRKTMKGRIRR 238
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Match: EDO49231 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGU4])

HSP 1 Score: 95.5153 bits (236), Expect = 4.582e-23
Identity = 71/217 (32.72%), Postives = 112/217 (51.61%), Query Frame = 1 
Query:  247 VIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAID-NRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTI--MPRVHANGSFIS-HPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFE 885
            VI  G  +T T S+  AL R+     Y + E I    +  D W++ I+  R  D E    +F N  A TD PG  F++++++  P+ KVILT R  E WL S +  +  +  + +   F+S  PI +       ++   M+   +      E+  I+ +A E +N++VRS++P  +LLE+ V +GW PLC FL     P E+FP  N   EF +R+E
Sbjct:    3 VICAGICKTATKSMAKAL-RILGYNVYDVEEQIC---ELFDDWLDLIEHGRLPDFEA---MFENVDAITDLPGNFFFEEILKASPDAKVILTVREQEAWLRSSKKQLKMVQGLFSKLWFLSLSPIYLG----IRKITNGMVAALYGTINA-ESTWIIAKALEMHNQRVRSTVPPGQLLEYSVTEGWGPLCDFLG-HATPKEKFPHENVKGEFTKRWE 206
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Match: EDO48573 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV3])

HSP 1 Score: 90.8929 bits (224), Expect = 1.831e-21
Identity = 66/206 (32.04%), Postives = 107/206 (51.94%), Query Frame = 1 
Query:  247 VIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKM---PRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVN 855
            +IG G  +TGT SL  AL  L     Y++ +     + H D +I A + +  + E    ++ N  ATTD P  +FYKD+ + +P+ KVIL+ R++E+   S R + +  +    S I++P+++  M   P   +      RH    + E E D      Y+ +N+ V ++I  ++LL F   DGW+PLC FL   E+P   +P VN
Sbjct:    3 IIGAGLPKTGTKSLASALRHL----GYNVHDRPEQVQFHCDEYIAAFEGQMPNFE---EMYENVDATTDVPACLFYKDIFRAFPDAKVILSVRDNEE---SWRRSFLKTLEVLSSMINNPLIILGMLLTPSGRKF--SRFRHNLIKYVENELD------YKKHNDAVMANIQAKQLLVFNPKDGWEPLCAFLG-AEVPTIPYPRVN 189
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Match: EDO48574 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV4])

HSP 1 Score: 92.0485 bits (227), Expect = 6.081e-21
Identity = 66/206 (32.04%), Postives = 109/206 (52.91%), Query Frame = 1 
Query:  244 LVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILV--KKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVN 855
            L+IG G  +TGT SL  AL  L     Y++ +     + H D +I A + +  + E    ++ N  ATTD P +IFYK++ + +P+ KVIL+ R+ E+   S R +++  V    S  ++P+L+  + +      + +++R      K    +EI    Y  +N  V ++IP ++LL F   DGW+PLC FL   E+P   +P VN
Sbjct:  157 LIIGAGLPKTGTKSLASALRHL----GYNVHDHPEQIQFHCDEYIAAFEGQMPNFE---EMYANVDATTDLPASIFYKEIFKAFPDAKVILSVRDDEE---SWRRSLLKTVGVVSSMTNNPLLIVCRTLTPTGRKYSRILRFIMNYLK----NEI---DYTKHNGDVMANIPAKQLLVFNPKDGWEPLCAFLG-AEVPAIPYPRVN 344
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Match: EDO41210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4T7])

HSP 1 Score: 88.1965 bits (217), Expect = 2.706e-20
Identity = 71/210 (33.81%), Postives = 107/210 (50.95%), Query Frame = 1 
Query:  247 VIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSED-WLTSVR-NTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEIL-KRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKE 867
            VI  G  +TGT SL  AL R+   K Y   E   +   H++ WI A +   +      R++ +  A TD P   +Y++L   +P  KVIL+ R++ D W  S+  N  + +   +   +   +L     +   +   M+  AF+      N  IL K+AY  +NE+V++ IPK++LL FKV  GW PLCRFL+I E P   FP+ N   E
Sbjct:   25 VICAGMPKTGTKSLAKAL-RILGLKVYDADE---HCIYHMEEWIQAFETSKMPDLY--RMYKDVDAVTDVPPMFWYEELHYAFPEAKVILSVRDNTDVWYESLLWNQKVLKEAMSLRVMLGFLLTPTGWKMSRMMSDMMNAAFKSTNP--NAAILNKKAYREHNERVKNVIPKDKLLVFKVEQGWPPLCRFLEIAE-PEIFFPYENVNSE 225
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Match: EDO33663 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRI4])

HSP 1 Score: 86.6557 bits (213), Expect = 3.102e-20
Identity = 61/207 (29.47%), Postives = 98/207 (47.34%), Query Frame = 1 
Query:  247 VIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSD--RIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEEL-HIKMIRHAFQPFKELENDEILKRAY-ETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVN 855
            +I  G  +TGT S+  AL R+     Y + E +    D    W + I    I+ +  D   +F +  A TDYPG+ F+++++  +P  KV+LT R+ + W  S+   I         +  + +L    P F +L  I +I               + R +   +N +VR ++PK++LL F V  GW+PLC FL    IP  +FP  N
Sbjct:    3 IICAGATKTGTKSMAKAL-RMLGYTVYDIAEQLGEFADE---WTDLI----IEGKLPDFKSMFHDIDAITDYPGSFFFEEILAAFPQAKVVLTVRDEDAWARSLSLEI---AETAKMWSKNSVLAFLSPTFAKLKRISLIMETAMCGNFTPKSTFIHRKFIHMHNNRVRINVPKDQLLVFSVTQGWQPLCDFLG-HAIPTPDFPHEN 197
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Match: SMESG000035068.1 (SMESG000035068.1)

HSP 1 Score: 464.537 bits (1194), Expect = 3.706e-167
Identity = 223/224 (99.55%), Postives = 224/224 (100.00%), Query Frame = 1 
Query:  220 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS 891
            MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNH+DHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS
Sbjct:    1 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHQDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS 224
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Match: SMESG000035060.1 (SMESG000035060.1)

HSP 1 Score: 249.595 bits (636), Expect = 5.502e-82
Identity = 115/221 (52.04%), Postives = 158/221 (71.49%), Query Frame = 1 
Query:  220 MKNENH-QLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNER 879
            ++ EN  +LLVIG GQMRTGT+SLK ALERL+  KCYHM+ L+N+HKDH++ W+NA++   +DKET+D +F  Y +  DYP + FY+DLI IYPN KVILTTR+++ W+ SV+ T M +      F+   ++   +P+F E+  KM    F   K+L  +E +  AYETYNE+VR  +PKERLLEF+V  GW+PLC FL I+EIP+E FP++N+ KEF ER
Sbjct:    4 LQTENKTELLVIGAGQMRTGTFSLKYALERLYGGKCYHMLALVNDHKDHIECWLNALNRGYLDKETADFLFNGYCSAIDYPSSAFYQDLINIYPNAKVILTTRSTKSWIESVKQTTMKKDFFYLIFLRLSMIFNGLPKFLEMKEKMFERVFHLSKDLRTEENMAIAYETYNEEVRKVVPKERLLEFEVRSGWEPLCTFLGIEEIPDEPFPYMNTAKEFRER 224
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Match: SMESG000035040.1 (SMESG000035040.1)

HSP 1 Score: 239.195 bits (609), Expect = 6.555e-78
Identity = 113/227 (49.78%), Postives = 162/227 (71.37%), Query Frame = 1 
Query:  220 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHP-ILVKKMPRFEELHIKMIRHAFQPFKE--LENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS 891
            M+ +NH+L VIG GQMRTGT SLK+ALE+L++ KCYHM E+  NH++H+ LWI+  D   +DK T++ +  +Y AT D+P    YKDL+++YPN KVILTTRNS+DWL SVRNT + +  ++ +F+S    +   + RF E   K+I HA++   E  + +D ILK+ YE +N++VR ++PKERLLEF    GW+PLC FL++  IP+E +PFVNST+EFN+   K+
Sbjct:    1 MRKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDKSTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKK-GSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNL-PIPDEPYPFVNSTEEFNKYINKT 225
[Close]
BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Match: SMESG000035061.1 (SMESG000035061.1)

HSP 1 Score: 231.106 bits (588), Expect = 6.692e-75
Identity = 116/221 (52.49%), Postives = 154/221 (69.68%), Query Frame = 1 
Query:  220 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQ-PFKELENDE-ILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNE 876
            MKN   +LLVIG GQMRTGT SLK ALE+L+  K YHM E+  N  DH+ LWI A+D   +D+E S+ IF  Y A  D P A FY+DLI+IYP+ KVILTTRNS+DW+ SV+ T + +     S     +  K + + E++ +K+  H+FQ P+K L+ND+ +LK ++E YNEKVR ++PKERLLEF   DGWKPLC FLD+  IPNE FPFVN+  E+++
Sbjct:    1 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVKQTTIIKNGLQLSLERFCLWNKGISKLEQMCMKIRWHSFQVPYKILQNDDKVLKESFEKYNEKVRQTVPKERLLEFNPKDGWKPLCEFLDL-PIPNESFPFVNNADEYDK 220
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BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Match: SMESG000035059.1 (SMESG000035059.1)

HSP 1 Score: 229.95 bits (585), Expect = 2.062e-74
Identity = 121/226 (53.54%), Postives = 152/226 (67.26%), Query Frame = 1 
Query:  220 MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVDLWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILTTRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAFQ-PFKELEN-DEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDIKEIPNEEFPFVNSTKEFNERFEKS 891
            M N    L VIG GQMRTGT SLK ALERL+  K YHM EL  N  DH+ LWI A+D   +D+E S  IF  Y A    P A FY+DLI+IYP+ KVILTTRNS+DW+ SVR T M +           +  K + RFEEL +K+  H+F   F  L+N DE+LK ++E +NEKVR ++ KERLLEFK  DGWKPLC+FLD+  IPNE FPFVN+++EF++R  +S
Sbjct:    1 MPNNGQNLFVIGAGQMRTGTTSLKYALERLYGGKIYHMDELYFNGVDHIGLWIRALDKGHLDRELSGEIFTGYCAAVGNPTASFYQDLIEIYPDAKVILTTRNSDDWIRSVRKTTMRKSELMKILERISLWNKGIFRFEELCVKLRWHSFHLQFDTLQNDDELLKESFERFNEKVRLTVSKERLLEFKPKDGWKPLCQFLDLP-IPNEPFPFVNNSEEFSKRIVRS 225
The following BLAST results are available for this feature:
BLAST of Nad dependent epimerase dehydratase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5P8I4L82.513e-7051.13Uncharacterized protein OS=Schmidtea mediterranea ... [more]
A0A2H1CLP51.241e-5140.52Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A4E0RFY72.203e-5139.83Cell wall integrity and stress response component ... [more]
A0A183AA273.429e-5140.25Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A183AA352.710e-4842.80Uncharacterized protein OS=Echinostoma caproni OX=... [more]
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO492314.582e-2332.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485731.831e-2132.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485746.081e-2132.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412102.706e-2033.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO336633.102e-2029.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Nad dependent epimerase dehydratase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nad dependent epimerase dehydratase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035068.13.706e-16799.55SMESG000035068.1[more]
SMESG000035060.15.502e-8252.04SMESG000035060.1[more]
SMESG000035040.16.555e-7849.78SMESG000035040.1[more]
SMESG000035061.16.692e-7552.49SMESG000035061.1[more]
SMESG000035059.12.062e-7453.54SMESG000035059.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026136 ID=SMED30026136|Name=Nad dependent epimerase dehydratase|organism=Schmidtea mediterranea sexual|type=transcript|length=993bp
CAGCAAATGACATAGTCAGTTTACATTTATAATAGCTATTTGATTAATTA
AGCTTTGAACACGTTTGAAACCGATAGCTTCACTATCAGTACCTATAAAT
CGCGTTCTGATTAAAATTGCGCACGAAAATAACTCGTATAAACAAAACTT
TCATCTAAAAATATTTATGTACTCTAATATATATAAAAGTCCAAGTTAAT
TTTAAATATTCATTAAAACATGAAGAACGAAAATCATCAACTGCTAGTGA
TTGGCGTTGGACAAATGAGAACTGGAACCTACTCTTTGAAGTTGGCACTT
GAAAGGTTATTCGACAAGAAGTGCTATCACATGATCGAGTTGATCAACAA
CCACAAAGACCATGTAGATCTATGGATAAATGCCATTGACAATCGGTCCA
TTGATAAGGAAACATCTGACAGAATATTTTGTAATTATTTTGCAACCACT
GATTACCCAGGTGCGATATTTTACAAAGATCTCATACAAATCTATCCGAA
TGTCAAAGTGATTCTTACAACTAGAAACAGTGAAGACTGGTTGACAAGTG
TCCGAAATACGATTATGCCTAGAGTACACGCGAATGGTAGCTTTATTAGC
CATCCAATTTTGGTGAAGAAAATGCCGAGATTTGAAGAACTTCACATAAA
AATGATACGACATGCTTTTCAACCGTTTAAAGAGTTGGAAAACGACGAAA
TTTTAAAGCGGGCATATGAAACTTACAACGAAAAGGTAAGAAGCAGTATT
CCCAAAGAAAGACTACTGGAATTTAAAGTTAATGATGGTTGGAAACCGTT
ATGCAGGTTTCTTGACATTAAAGAAATACCAAATGAGGAGTTTCCGTTTG
TCAATTCGACAAAAGAATTTAACGAGAGATTTGAAAAGTCTTAAAGGAAT
TATTTCCTACTGACGTCGCCTTCTCGGAAAAATTGTCAACATAGAATAAA
TTATTTTTAAATTAAATCTTATATTATAGTTTTTTTTAATTTT
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protein sequence of SMED30026136-orf-1

>SMED30026136-orf-1 ID=SMED30026136-orf-1|Name=SMED30026136-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=225bp
MKNENHQLLVIGVGQMRTGTYSLKLALERLFDKKCYHMIELINNHKDHVD
LWINAIDNRSIDKETSDRIFCNYFATTDYPGAIFYKDLIQIYPNVKVILT
TRNSEDWLTSVRNTIMPRVHANGSFISHPILVKKMPRFEELHIKMIRHAF
QPFKELENDEILKRAYETYNEKVRSSIPKERLLEFKVNDGWKPLCRFLDI
KEIPNEEFPFVNSTKEFNERFEKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
Vocabulary: INTERPRO
TermDefinition
IPR040632Sulfotransfer_4
IPR027417P-loop_NTPase
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..224
e-value: 2.4E-67
score: 229.2
NoneNo IPR availablePANTHERPTHR36978FAMILY NOT NAMEDcoord: 5..220
IPR040632Sulfotransferase, S. mansonii-typePFAMPF17784Sulfotransfer_4coord: 10..217
e-value: 7.9E-61
score: 205.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 9..218