SMED30026135

Overview
NameSMED30026135
Smed IDSMED30026135
Length (bp)805
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30026135 (SMED30026135) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30026135 (SMED30026135) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30026135 (SMED30026135) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30026135 (SMED30026135) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026135

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30026135h1SMcG0020893 SmedASXL_001721SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30026135h1SMcG0020893 dd_Smed_v4_2807_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026135h1SMcG0020893 Contig49000newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30026135h1SMcG0020893 Contig49000uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30026135 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.029237:5874..8480 -3943
v31.020362:7671..10045 +3925
Homology
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BLAST of SMED30026135 vs. Ensembl Human
Match: GADD45GIP1 (GADD45G interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:29996])

HSP 1 Score: 48.521 bits (114), Expect = 3.070e-6
Identity = 32/127 (25.20%), Postives = 70/127 (55.12%), Query Frame = 2 
Query:  344 YQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKEM 724
            Y    +A YG  S + P  + WPSP+QL  L+  E  +      + +++++++L +++   E +  I + +  +P+MI +++ +Q +  + A+  ++ R  +  EA+E  G+ +   DPR  +F+E+
Sbjct:   56 YAAKQFARYGAASGVVPGSL-WPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQV---DPRSARFQEL 178
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BLAST of SMED30026135 vs. Ensembl Zebrafish
Match: gadd45gip1 (growth arrest and DNA-damage-inducible, gamma interacting protein 1 [Source:ZFIN;Acc:ZDB-GENE-091204-282])

HSP 1 Score: 57.7658 bits (138), Expect = 1.758e-9
Identity = 36/133 (27.07%), Postives = 75/133 (56.39%), Query Frame = 2 
Query:  326 YETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKEM 724
            ++ T  ++R  +  YG  S ++P ++ WPS  +LE L   E  +      +++ +   ++ K+K   E +  I +++  +PK+I  +K ++ +A + A ++++ +  ++  ARE +GF++   DPR  KFKEM
Sbjct:   76 WQKTEKFERRLFGRYGRASGVNPVKL-WPSAARLEELMAEEREWHPPVEQMLQNIAERQMEKEKRRIEREKTIAESMAKMPKLIADWKKQKREAKQKANEEKQKQVRLLAMARERFGFAV---DPRSVKFKEM 204
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BLAST of SMED30026135 vs. TrEMBL
Match: A0A3S5CJA9 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS6340 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.665e-17
Identity = 58/205 (28.29%), Postives = 106/205 (51.71%), Query Frame = 2 
Query:  131 RYKDYKKVWKKEGILDDWQNLGFRIPSITERWESE--------NNPVTDISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKE 721
            R + Y   W+    L D+QNL    PS+ E+W SE             DI+RLP+HL++R    ++   P E+  T +DY+R  YA YG  S +DP+E+ +P+ + ++  + +E  +E    ++I   K EK   +  + +    I + +E +PK+I+     Q    ++   +E   ++++ EAR+ +G+ + I D +  K K+
Sbjct:   20 RGRKYYINWRSHDQLTDYQNLTRGWPSVYEQWHSELKDSILPSEFQALDINRLPRHLRSRSKCKVEPFLPIEVEGTPLDYKRRLYALYGSSSLVDPSEL-FPTSRDIKYERLKESQFERPVMEVINEKKDEKEAFKLKVAQTMKNIQREMEKMPKLIEKLNSHQAALRENKILRESKNKELLEEARDRFGYRVDIRDEKFLKLKQ 223
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BLAST of SMED30026135 vs. TrEMBL
Match: A0A5J4NJE6 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0000034 PE=4 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.634e-16
Identity = 66/215 (30.70%), Postives = 112/215 (52.09%), Query Frame = 2 
Query:  104 PKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWE---------SENNPVTDISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDN-YEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFK 718
            P  +     RYK Y + W     LDD+Q +    P+  E W+          E  P  DISRLP HLK RK    +    +   E+T+DY+RA +  YG +S +DP+ + +  PK L   K  +   ++   +++++  K +K  + K I+E    I KNL+T+P++I  ++ RQ K    +E + + ++ ++ EAR+ +G+ +   DPR  KF+
Sbjct:   11 PSAVCWLLRRYKKYYRNWWIPYSLDDFQTVTQSRPNPYEAWDREWKMSQLPKEWQPF-DISRLPNHLKARKRPTKEFFSIQRPVESTLDYRRALFGRYGLRSGVDPSHL-FVDPKMLAFEKALDAELHKQTISEILEVNKQKKAEEAKEIDELMKTIKKNLQTMPELIDKFQTRQSKQYAVSEVEVQRKKAIVEEARDRFGYYV---DPRDPKFQ 220
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BLAST of SMED30026135 vs. TrEMBL
Match: A0A504YCA2 (Growth arrest and DNA damage-inducible proteins-interacting protein 1 OS=Fasciola gigantica OX=46835 GN=FGIG_10689 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.353e-14
Identity = 62/225 (27.56%), Postives = 108/225 (48.00%), Query Frame = 2 
Query:   74 MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWESENNPVT---------DISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKE 721
            M H++  S  PK +R    R K Y   W     LDD+QN+    P+  E W+ E              DIS LP+HLK R     +  QPK   E ++DY+R  +  YG  S +DPA + +  P+ +   +  E        ++++  +  +    +A E+    I  +L  +P+++  Y+ +Q K  +  E +E  ++ ++ EAR+ +G+ +S  DP+  K +E
Sbjct:    1 MLHLITGSCFPKSVRFCPTRTKKYYPAWYIPYCLDDYQNVTRSKPNPYEMWDREWKAAGLKTRELQPFDISLLPQHLKLRLKPTREFFQPKYRAELSLDYKRRLFGVYGLASGVDPAAL-FVEPRTVSFDQAVEQATTRPIPEVLEERRRAEREASEAEEKLMKNIKTSLTKMPELLTKYEAQQQKQAEQIETRELKKKAILEEARDRFGYYVSPQDPKFKKLRE 224
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BLAST of SMED30026135 vs. TrEMBL
Match: A0A4S2LBF8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008830 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.201e-13
Identity = 65/224 (29.02%), Postives = 106/224 (47.32%), Query Frame = 2 
Query:   74 MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWESE----NNPVT----DISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKE 721
            M    C   AP V +    R+K Y   WK   ILDD+Q      PS  E W  E    N P      DISRLP HLK R              E+T+DY+R  +  YG  S +DP+ + +  P+  +     E   E    ++++  K  K+   + + E    I  N++ +P++I+ Y+ ++ K    +E +E+ R+ ++ EAR+ +G+ +   DP+  K K+
Sbjct:   33 MRGAACWLCAPSV-QILPCRFKKYYPSWKTPFILDDFQTFTQSSPSSYEEWNREWKKSNLPKALQSFDISRLPSHLKKRLRPTEDFFIENSPVESTLDYKRRLFGEYGFISGVDPS-ILFVDPEVAKFESALEQATERPIEEVLEEAKRAKIAFDELVNERVKNIKSNMKKMPELIEKYELQKSKEDSISEVEEQKRRAVLEEARDRFGYYLDPRDPKFIKLKQ 254
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BLAST of SMED30026135 vs. TrEMBL
Match: H2KSZ9 (Growth arrest and DNA damage-inducible proteins-interacting protein 1 (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_110984 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 3.513e-13
Identity = 62/208 (29.81%), Postives = 103/208 (49.52%), Query Frame = 2 
Query:  131 RYKDYKKVWKKEGILDDWQNLGFRIPSITERWESE----NNPVT----DISRLPKHLKNR---KLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKE 721
            R+K Y   WK   ILDD+Q      PS  E W  E    N P      DISRLP HLK R     D    S P    E+T+DY+R  +  YG  S +DP+ + +  P+  +     E   E    ++++  K  K+   + + E    I  N++ +P++I+ ++ ++ K    +E +E+ R+ ++ EAR+ +G+ +   DP+  K K+
Sbjct:    6 RFKKYYPSWKTPFILDDFQTFTQSSPSSYEEWNREWKKSNLPKALQSFDISRLPSHLKKRLRPTKDFFIESSP---VESTLDYKRRLFGEYGFISGVDPS-ILFVDPEVAKFESALEKATERPIEEVLEEAKRAKVAFDELVNERIKNIKSNMKKMPELIEKHELQKSKQHSISELEEQKRKAVLEEARDRFGYYLDPRDPKFIKLKQ 209
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BLAST of SMED30026135 vs. Ensembl Cavefish
Match: gadd45gip1 (GADD45G interacting protein 1 [Source:NCBI gene;Acc:103024491])

HSP 1 Score: 63.5438 bits (153), Expect = 1.366e-11
Identity = 40/133 (30.08%), Postives = 68/133 (51.13%), Query Frame = 2 
Query:  326 YETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKEM 724
            ++ T  Y R  +A YG  S I PA + WPSP +L+ L   E  +     D+++ +  E+  K       +  I  N+  +PKMI  +K  + +  +  ++ +  ++ ++ EARE +G +I   DPR  KF EM
Sbjct:   63 WQKTERYDRRLFARYGSASGISPAAL-WPSPARLQELISEEKQWNPPLEDMLQNIAAEEKEKAAKRRAREQLIAANMAKMPKMIADWKKEKQEMKRKQKEDKAKKERLLAEARERFGHAI---DPRSPKFLEM 191
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BLAST of SMED30026135 vs. Ensembl Medaka
Match: gadd45gip1 (GADD45G interacting protein 1 [Source:NCBI gene;Acc:101164314])

HSP 1 Score: 56.9954 bits (136), Expect = 2.114e-9
Identity = 35/133 (26.32%), Postives = 71/133 (53.38%), Query Frame = 2 
Query:  326 YETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKEM 724
            ++ +  Y R  +  YG  S IDPA + WPS  +L+ +   E+ +      ++K ++  +  + +   E +  I  N+  +PKM+  ++  + +A +  ++++  R+ ++ EARE +G ++   DPR  KF EM
Sbjct:   61 WQKSAKYDRKLFGRYGSASGIDPASL-WPSHAELDKIIAEENEWHPPLEVMLKNIEAREKEEAEKRREKEKIIAANMAKMPKMVADWRREKREAKQKQKEEKARREKLLAEARERFGSTM---DPRSPKFLEM 189
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BLAST of SMED30026135 vs. Planmine SMEST
Match: SMESG000016933.1 (SMESG000016933.1)

HSP 1 Score: 416.772 bits (1070), Expect = 4.169e-149
Identity = 214/217 (98.62%), Postives = 216/217 (99.54%), Query Frame = 2 
Query:   74 MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWESENNPVTDISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAIEEYQNQIGKNLETLPKMIQSYKDRQXXXXXXXXXXXXXRQDMIREAREYYGFSISIGDPRMDKFKEM 724
            MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWESENNPVTDISRLPKHLKNRKLDILQISQP+ELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLI TVKMEKLMKQKAIEEYQNQIGKNLE+LPKMIQSYKDRQDKAIKDAEKKEKARQDMIREAREYYGFSISIGDPRMDKFKEM
Sbjct:    1 MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITERWESENNPVTDISRLPKHLKNRKLDILQISQPRELYETTVDYQRATYAFYGEKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLINTVKMEKLMKQKAIEEYQNQIGKNLESLPKMIQSYKDRQDKAIKDAEKKEKARQDMIREAREYYGFSISIGDPRMDKFKEM 217
The following BLAST results are available for this feature:
BLAST of SMED30026135 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
GADD45GIP13.070e-625.20GADD45G interacting protein 1 [Source:HGNC Symbol;... [more]
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BLAST of SMED30026135 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gadd45gip11.758e-927.07growth arrest and DNA-damage-inducible, gamma inte... [more]
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BLAST of SMED30026135 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S5CJA91.665e-1728.29Uncharacterized protein OS=Protopolystoma xenopodi... [more]
A0A5J4NJE61.634e-1630.70Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A504YCA27.353e-1427.56Growth arrest and DNA damage-inducible proteins-in... [more]
A0A4S2LBF82.201e-1329.02Uncharacterized protein OS=Opisthorchis felineus O... [more]
H2KSZ93.513e-1329.81Growth arrest and DNA damage-inducible proteins-in... [more]
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BLAST of SMED30026135 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gadd45gip11.366e-1130.08GADD45G interacting protein 1 [Source:NCBI gene;Ac... [more]
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BLAST of SMED30026135 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30026135 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gadd45gip12.114e-926.32GADD45G interacting protein 1 [Source:NCBI gene;Ac... [more]
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BLAST of SMED30026135 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016933.14.169e-14998.62SMESG000016933.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026135 ID=SMED30026135|Name=SMED30026135|organism=Schmidtea mediterranea sexual|type=transcript|length=805bp
ACGAAATATTTAAAATTCATGATAATTAAACCAATTATAATAATTCTATT
AATTTAAGTAGCTTTTATTTTAAATGTTTCATATACTTTGCAAATCATTT
GCTCCTAAAGTATTACGTCGATATACTCACAGATATAAGGATTATAAAAA
AGTTTGGAAAAAAGAGGGAATATTAGATGATTGGCAGAATCTGGGATTCA
GAATCCCTTCCATTACAGAGAGATGGGAAAGCGAAAATAATCCTGTCACT
GATATAAGCAGACTTCCGAAACACTTGAAAAATAGAAAGTTAGACATTTT
ACAAATATCTCAACCGAAGGAATTATACGAAACAACTGTGGATTATCAAA
GAGCAACTTATGCATTTTACGGGGAAAAAAGCGAAATAGACCCAGCCGAA
GTTTGTTGGCCATCGCCGAAACAATTGGAATTACTAAAAGATAGAGAGGA
CAATTATGAGGATAAATTTACGGATTTAATAAAAACTGTTAAAATGGAAA
AATTAATGAAACAAAAAGCTATCGAAGAATACCAAAATCAGATCGGGAAA
AACCTTGAAACTTTACCGAAAATGATCCAATCGTACAAAGATAGACAAGA
CAAAGCCATTAAAGATGCCGAAAAGAAAGAAAAAGCTCGGCAGGATATGA
TCCGTGAGGCGCGCGAGTATTACGGGTTCTCCATATCGATCGGTGATCCG
AGAATGGACAAGTTCAAGGAAATGAAATTACTAGAGGCTAAAAAGGTTGC
CAAGGACGCCAAGAAGCGAATGAAATTGATGAAATAAATGCTTTTATTGA
AAATA
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protein sequence of SMED30026135-orf-1

>SMED30026135-orf-1 ID=SMED30026135-orf-1|Name=SMED30026135-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=238bp
MFHILCKSFAPKVLRRYTHRYKDYKKVWKKEGILDDWQNLGFRIPSITER
WESENNPVTDISRLPKHLKNRKLDILQISQPKELYETTVDYQRATYAFYG
EKSEIDPAEVCWPSPKQLELLKDREDNYEDKFTDLIKTVKMEKLMKQKAI
EEYQNQIGKNLETLPKMIQSYKDRQDKAIKDAEKKEKARQDMIREAREYY
GFSISIGDPRMDKFKEMKLLEAKKVAKDAKKRMKLMK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR043035Damage-induce-interact_sf
IPR018472Damage-induce-interacting_prot
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0007049cell cycle
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 219..237
NoneNo IPR availableCOILSCoilCoilcoord: 118..138
NoneNo IPR availableCOILSCoilCoilcoord: 168..188
IPR043035Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamilyGENE3DG3DSA:1.20.58.1430coord: 65..192
e-value: 6.1E-18
score: 66.9
IPR018472Growth arrest/ DNA-damage-inducible protein-interacting protein 1PFAMPF10147CR6_interactcoord: 51..236
e-value: 2.0E-25
score: 89.7
IPR018472Growth arrest/ DNA-damage-inducible protein-interacting protein 1PANTHERPTHR31761FAMILY NOT NAMEDcoord: 62..234