MIEAP domain-containing protein

Overview
NameMIEAP domain-containing protein
Smed IDSMED30026086
Length (bp)1760
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MIEAP domain-containing protein (SMED30026086) t-SNE clustered cells

Violin plots show distribution of expression levels for MIEAP domain-containing protein (SMED30026086) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MIEAP domain-containing protein (SMED30026086) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MIEAP domain-containing protein (SMED30026086) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026086

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30026086h1SMcG0011052 SmedASXL_013847SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30026086h1SMcG0011052 dd_Smed_v4_14351_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30026086h1SMcG0011052 dd_Smed_v4_14351_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30026086h1SMcG0011052 dd_Smed_v4_14351_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30026086h1SMcG0011052 dd_Smed_v4_14351_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30026086h1SMcG0011052 dd_Smed_v6_14351_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 89.7373 bits (221), Expect = 2.887e-18
Identity = 59/191 (30.89%), Postives = 97/191 (50.79%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRET--ISRSVLKNIRTQI--------IYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +I  +  L   +  +     +VL  +   +        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA TR
Sbjct:  301 ALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIY-IATVEAFHVAKMAFRH-FKIHVRKSLTPSYVGSNDFENAVLDYVICHLDLYDSQSSVNDVIRAMNVNPKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAYGADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVTR 489

HSP 2 Score: 55.8398 bits (133), Expect = 1.510e-7
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNV 428
            E+L    +      LQEKL+ W  E+  N  + N++ C ELIE  A++Q QLF +L      GG       +K++L+P+L   +    L  +V
Sbjct:    3 ENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSV 95
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 89.7373 bits (221), Expect = 3.083e-18
Identity = 59/191 (30.89%), Postives = 97/191 (50.79%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRET--ISRSVLKNIRTQI--------IYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +I  +  L   +  +     +VL  +   +        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA TR
Sbjct:  269 ALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIY-IATVEAFHVAKMAFRH-FKIHVRKSLTPSYVGSNDFENAVLDYVICHLDLYDSQSSVNDVIRAMNVNPKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAYGADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVTR 457

HSP 2 Score: 56.225 bits (134), Expect = 1.274e-7
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNV 428
            E+L    +      LQEKL+ W  E+  N  + N++ C ELIE  A++Q QLF +L      GG       +K++L+P+L   +    L  +V
Sbjct:    3 ENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSV 95
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 89.3521 bits (220), Expect = 3.576e-18
Identity = 59/191 (30.89%), Postives = 97/191 (50.79%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRET--ISRSVLKNIRTQI--------IYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +I  +  L   +  +     +VL  +   +        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA TR
Sbjct:  301 ALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIY-IATVEAFHVAKMAFRH-FKIHVRKSLTPSYVGSNDFENAVLDYVICHLDLYDSQSSVNDVIRAMNVNPKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAYGADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVTR 489

HSP 2 Score: 56.225 bits (134), Expect = 1.240e-7
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNV 428
            E+L    +      LQEKL+ W  E+  N  + N++ C ELIE  A++Q QLF +L      GG       +K++L+P+L   +    L  +V
Sbjct:    3 ENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSV 95
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 53.1434 bits (126), Expect = 3.959e-8
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNV 428
            E+L    +      LQEKL+ W  E+  N  + N++ C ELIE  A++Q QLF +L      GG       +K++L+P+L   +    L  +V
Sbjct:    3 ENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSV 95
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Zebrafish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:497395])

HSP 1 Score: 110.153 bits (274), Expect = 3.632e-25
Identity = 63/189 (33.33%), Postives = 100/189 (52.91%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSV---------LKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            LV R++DL++  R+ +   L  Y DD E  QRILF   + S +      R  ++R+R      H   E++  +V         L +++T I  D+IN      R   P  + FV +   I    ++ +        L+L  ++DGE++ + KYRRS+DS+FTAPLV+ H+WP L++   V++KGEA TR
Sbjct:  259 LVSRFSDLYATERLESQSLLLKYIDDLETVQRILFIAAVESFQAAKLAYRQFKSRVRKTLSSTHIGPESLEDAVVDYIVRNLDLYDVQTSI-NDVINAMNVNPRISFPPEVDFVLISSFIREACRIAFAMQTLDPPLDLAFSSDGELYSDVKYRRSFDSEFTAPLVAFHVWPALLEGDTVILKGEAVTR 446

HSP 2 Score: 67.781 bits (164), Expect = 1.841e-11
Identity = 30/102 (29.41%), Postives = 61/102 (59.80%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDY--FDNGLTSNVVMNILNE 449
            ++L    N++ + +LQ+KL+ W  ++     + N+++CCEL+EL ++IQ QLF +L      GG       LK++L+P+LG  +    + ++S+  ++++ E
Sbjct:    3 DTLRRLVNSSPYSVLQDKLDAWYKDYHVYSCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYSGVDTLKSRLLPWLGSCFTIAASAVSSDTSLSLIQE 104
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000051452.1 (spermatogenesis associated 18 [Source:NCBI gene;Acc:100379837])

HSP 1 Score: 109.383 bits (272), Expect = 7.189e-25
Identity = 63/188 (33.51%), Postives = 97/188 (51.60%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-------INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L+ R+ND+F+  R+ A   LR Y DD E  QRI+F   + S        R  R R+R      H   E++  +V+  I R   +YD+       I+      +   P ++ F+ +   I    ++ +        L++    DGE+F E KYRR+YDS+ TAPLV +H+WP L++N  V+VKGEA T+
Sbjct:  234 LISRFNDIFANDRLDAQTLLRRYIDDLEMVQRIIFIATVESFHAAKMAFRQFRLRVRKSLSPSHMGPESLEDTVIDYIVRNLDLYDVQSSVNEVISAMNVNPKISFPPDVDFILISAFIREVCRVAFAMQTLDPPLDIAFTVDGELFTESKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEALTK 421

HSP 2 Score: 69.3218 bits (168), Expect = 6.554e-12
Identity = 31/81 (38.27%), Postives = 52/81 (64.20%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L   AN+    +LQ+KL++W  ++  N  ++N++ CCEL+ELN++IQ QLFK+L      GG       LK++ +P+LG
Sbjct:   27 DTLRRLANSEKCRVLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKIQGQLFKILSVTAREGGQYAGVETLKSRFLPWLG 107
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000051448.1 (spermatogenesis associated 18 [Source:NCBI gene;Acc:100379837])

HSP 1 Score: 109.768 bits (273), Expect = 9.070e-25
Identity = 63/188 (33.51%), Postives = 97/188 (51.60%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-------INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L+ R+ND+F+  R+ A   LR Y DD E  QRI+F   + S        R  R R+R      H   E++  +V+  I R   +YD+       I+      +   P ++ F+ +   I    ++ +        L++    DGE+F E KYRR+YDS+ TAPLV +H+WP L++N  V+VKGEA T+
Sbjct:  277 LISRFNDIFANDRLDAQTLLRRYIDDLEMVQRIIFIATVESFHAAKMAFRQFRLRVRKSLSPSHMGPESLEDTVIDYIVRNLDLYDVQSSVNEVISAMNVNPKISFPPDVDFILISAFIREVCRVAFAMQTLDPPLDIAFTVDGELFTESKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEALTK 464

HSP 2 Score: 69.707 bits (169), Expect = 6.416e-12
Identity = 31/81 (38.27%), Postives = 52/81 (64.20%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L   AN+    +LQ+KL++W  ++  N  ++N++ CCEL+ELN++IQ QLFK+L      GG       LK++ +P+LG
Sbjct:   27 DTLRRLANSEKCRVLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKIQGQLFKILSVTAREGGQYAGVETLKSRFLPWLG 107
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: itga8 (integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GENE-961825])

HSP 1 Score: 101.679 bits (252), Expect = 2.637e-22
Identity = 62/204 (30.39%), Postives = 105/204 (51.47%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-------INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMICSVQVRNRNV 1376
            L+ R+N++F+  R+ A   LR Y +D +  QRI+F   + S +      R  R R+R          E++  +V+  I R   ++D+       I       +   P  + F+ + G I    ++ +        L++   ADGE+F + KYRR+YDS+ TAPLV +H+WP L++N  V+VKGEA T+    +  SV+ R+++ 
Sbjct:  253 LIFRFNNIFANDRLDAQTLLRRYIEDLDMVQRIIFIATVESFRAAKMAFRQFRLRVRKSLSPSQMGPESLEDAVIDYIVRNLDLFDVQSSVNEVIGAMNVNPKISFPPEVDFILISGFIREVCRVAFAMQTLDPPLDIAFTADGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVTK-RGALWSSVRSRSKSA 455

HSP 2 Score: 67.3958 bits (163), Expect = 2.938e-11
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L   AN+    +LQ+KL+NW  ++  N  ++N++ CCEL+ELN+ +Q QLF++L      GG       +K++ +P+LG
Sbjct:    3 DTLRRLANSEKCRVLQDKLDNWYKDYHINSCDSNLNVCCELLELNSNVQGQLFQILSVTAREGGQYAGVETIKSRFLPWLG 83
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: itga8 (integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GENE-961825])

HSP 1 Score: 101.679 bits (252), Expect = 3.223e-22
Identity = 59/188 (31.38%), Postives = 97/188 (51.60%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-------INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L+ R+N++F+  R+ A   LR Y +D +  QRI+F   + S +      R  R R+R          E++  +V+  I R   ++D+       I       +   P  + F+ + G I    ++ +        L++   ADGE+F + KYRR+YDS+ TAPLV +H+WP L++N  V+VKGEA T+
Sbjct:  249 LIFRFNNIFANDRLDAQTLLRRYIEDLDMVQRIIFIATVESFRAAKMAFRQFRLRVRKSLSPSQMGPESLEDAVIDYIVRNLDLFDVQSSVNEVIGAMNVNPKISFPPEVDFILISGFIREVCRVAFAMQTLDPPLDIAFTADGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVTK 436

HSP 2 Score: 67.0106 bits (162), Expect = 4.494e-11
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L   AN+    +LQ+KL+NW  ++  N  ++N++ CCEL+ELN+ +Q QLF++L      GG       +K++ +P+LG
Sbjct:    3 DTLRRLANSEKCRVLQDKLDNWYKDYHINSCDSNLNVCCELLELNSNVQGQLFQILSVTAREGGQYAGVETIKSRFLPWLG 83
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 92.8189 bits (229), Expect = 4.618e-21
Identity = 59/192 (30.73%), Postives = 98/192 (51.04%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQ-------FRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +IR +  L          F   +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA T+
Sbjct:    9 ALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIY-IATVEAFHVAKMAFRH-FKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTK 198
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 93.2041 bits (230), Expect = 1.233e-19
Identity = 67/225 (29.78%), Postives = 107/225 (47.56%), Query Frame = 3 
Query:  687 NDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQ-------FRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            N   T  A I+S  P     S V           L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +IR +  L          F   +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA T+
Sbjct:  240 NSPCTTVAKIRSPSPNRAKMSSVAR------KAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIY-IATVEAFHVAKMAFRH-FKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTK 456

HSP 2 Score: 52.7582 bits (125), Expect = 8.221e-7
Identity = 42/146 (28.77%), Postives = 67/146 (45.89%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGG-LEGSEIKLKNQLIPYLGKDY--FDNGLTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGIYDRLDELE 578
            ESL   A +     LQ+K+  W +++  N  + N++ C ELIE  A++Q QLF +L      GG  EG E  +K +L+P L   +   + G T+   M    +    +K     P   ++    +N  F E    Q + D L  L+
Sbjct:    3 ESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELIEQVAKVQAQLFGILTVTAQEGGNNEGVET-IKCRLLPLLQTSFSSVNMGKTAESEMCATQDFQLRSKNRDNSPDQDQH--QSDNESFSETQPTQ-VQDELKSLQ 144
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 92.8189 bits (229), Expect = 1.362e-19
Identity = 67/225 (29.78%), Postives = 107/225 (47.56%), Query Frame = 3 
Query:  687 NDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQ-------FRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            N   T  A I+S  P     S V           L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +IR +  L          F   +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA T+
Sbjct:  158 NSPCTTVAKIRSPSPNRAKMSSVAR------KAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIY-IATVEAFHVAKMAFRH-FKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTK 374
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 92.8189 bits (229), Expect = 1.898e-19
Identity = 67/225 (29.78%), Postives = 107/225 (47.56%), Query Frame = 3 
Query:  687 NDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQ-------FRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            N   T  A I+S  P     S V           L+ R++D +S++R+ A   LR   D  E  QRI++ +  + A  V++    H  +IR +  L          F   +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA T+
Sbjct:  272 NSPCTTVAKIRSPSPNRAKMSSVAR------KAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIY-IATVEAFHVAKMAFRH-FKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTK 488

HSP 2 Score: 52.373 bits (124), Expect = 1.385e-6
Identity = 30/85 (35.29%), Postives = 46/85 (54.12%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGG-LEGSEIKLKNQLIPYLGKDY 401
            ESL   A +     LQ+K+  W +++  N  + N++ C ELIE  A++Q QLF +L      GG  EG E  +K +L+P L   +
Sbjct:    3 ESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELIEQVAKVQAQLFGILTVTAQEGGNNEGVET-IKCRLLPLLQTSF 86
[Close]
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q503Q1|MIEAP_DANRE (Mitochondria-eating protein OS=Danio rerio OX=7955 GN=spata18 PE=2 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 2.742e-24
Identity = 63/189 (33.33%), Postives = 100/189 (52.91%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSV---------LKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            LV R++DL++  R+ +   L  Y DD E  QRILF   + S +      R  ++R+R      H   E++  +V         L +++T I  D+IN      R   P  + FV +   I    ++ +        L+L  ++DGE++ + KYRRS+DS+FTAPLV+ H+WP L++   V++KGEA TR
Sbjct:  259 LVSRFSDLYATERLESQSLLLKYIDDLETVQRILFIAAVESFQAAKLAYRQFKSRVRKTLSSTHIGPESLEDAVVDYIVRNLDLYDVQTSI-NDVINAMNVNPRISFPPEVDFVLISSFIREACRIAFAMQTLDPPLDLAFSSDGELYSDVKYRRSFDSEFTAPLVAFHVWPALLEGDTVILKGEAVTR 446

HSP 2 Score: 67.781 bits (164), Expect = 1.390e-10
Identity = 30/102 (29.41%), Postives = 61/102 (59.80%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDY--FDNGLTSNVVMNILNE 449
            ++L    N++ + +LQ+KL+ W  ++     + N+++CCEL+EL ++IQ QLF +L      GG       LK++L+P+LG  +    + ++S+  ++++ E
Sbjct:    3 DTLRRLVNSSPYSVLQDKLDAWYKDYHVYSCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYSGVDTLKSRLLPWLGSCFTIAASAVSSDTSLSLIQE 104
[Close]
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q498J5|MIEAP_XENLA (Mitochondria-eating protein OS=Xenopus laevis OX=8355 GN=spata18 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.569e-21
Identity = 59/188 (31.38%), Postives = 95/188 (50.53%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQI-IYDL-------INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L+ R+ND+F+  R+ A   LR Y  D +  QRI+F   + S        R  R R+       H   E++  +V+  I   + +YD+       I+      +   P  + F+ + G I    ++ +        L++    DGE+F + KYRR+YDS+ TAPLV +H+WP L++N  V+VKGEA T+
Sbjct:  253 LISRFNDIFANDRLDAQTLLRRYIQDLDMVQRIIFIATVESFHSAKMAFRQFRLRVCKSLSPSHMGPESLEDAVIDYIVGNLDLYDVQSSVNEVISAMNVNPKISFPPEVDFILISGFIQEVCRVAFTMQTLDPPLDIAFTVDGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVTK 440

HSP 2 Score: 65.0846 bits (157), Expect = 9.274e-10
Identity = 28/79 (35.44%), Postives = 49/79 (62.03%), Query Frame = 3 
Query:  156 LNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            L    N+    +LQ+KL++W  ++  N  ++N++ CCEL+ELN+++Q QLFK+L      GG       +K++ +P+LG
Sbjct:    5 LRRLTNSERCRLLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKVQGQLFKILSVTAQEGGQYAGVETIKSRFLPWLG 83
[Close]
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6AYL6|MIEAP_RAT (Mitochondria-eating protein OS=Rattus norvegicus OX=10116 GN=Spata18 PE=1 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.812e-19
Identity = 58/189 (30.69%), Postives = 94/189 (49.74%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIH----QFRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S++R+ A   LR   D  E  QRI++   + +        R  + R+R      H     F   +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F+E KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA T+
Sbjct:  319 ALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHIAKMAFRHFKIRVRKMLTPSHVGSNDFETAVSDYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEVFNEAKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTK 507

HSP 2 Score: 54.6842 bits (130), Expect = 1.625e-6
Identity = 31/90 (34.44%), Postives = 51/90 (56.67%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGL-EGSEIKLKNQLIPYLGKDYFDNGL 416
            E+L   A +  F  LQ+K+  W ++++ N  + N++ C ELIE  A++Q QLF +L      GG  EG E  +K++L+P L   +   G+
Sbjct:    3 ENLKKLAQSESFLSLQDKVCFWANDYQTNSCDQNLNCCIELIEQVAKVQAQLFAILTATAQEGGHNEGVET-IKSRLLPLLQTSFSSLGV 91
[Close]
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|E1BW58|MIEAP_CHICK (Mitochondria-eating protein OS=Gallus gallus OX=9031 GN=SPATA18 PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.204e-19
Identity = 60/188 (31.91%), Postives = 94/188 (50.00%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDLINNRGSYYR-FQIPQNLS------FVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            LV R+ D++S+ R  A   LR Y  D E  QRI++   + S        R  + R+R    + H   E++  +V+  I R + +YD+  +     R   I   +S      F  +   I    ++ ++       L++    DGE F E KY RS DSD+TA LV++H+WP L++N  V+VKGEA T+
Sbjct:  261 LVARFGDIYSKDRFDAERILRTYISDMEMVQRIIYTAAVESFHAAKMAYRQFKMRVRKTLSIGHSGPESLEDTVMDYIVRHEDLYDVQASVNEVIRSMNINPKISSTPECDFAVISSFIRELCRVAFSMQTLTPPLDVAFGMDGEFFSETKYHRSVDSDYTAALVAYHVWPALMENDVVIVKGEAVTK 448

HSP 2 Score: 61.2326 bits (147), Expect = 1.285e-8
Identity = 35/112 (31.25%), Postives = 60/112 (53.57%), Query Frame = 3 
Query:  153 SLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFC----GIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMN--ILNEVGKHTKR 470
            SL   A      ++QEKL +W  +++ N  + N+++CCELIE+ + IQ QLF +L       G Y G++     +K +L+P+LG  +F +  +  +      LN+    T+R
Sbjct:    4 SLKKLAKAESCRLMQEKLESWSKDYEINSCDQNLNQCCELIEMTSVIQGQLFTILNETSRESGHYAGVD----TIKTRLLPWLGT-WFSHATSGRLFETGLFLNQDSTETER 110
[Close]
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|E1BLK7|MIEAP_BOVIN (Mitochondria-eating protein OS=Bos taurus OX=9913 GN=SPATA18 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 7.447e-19
Identity = 60/191 (31.41%), Postives = 99/191 (51.83%), Query Frame = 3 
Query:  786 GLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELI------HQFRETISRSVLKNI----RTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
             L+ R++D +S+ R+ A   LR   D  E  QRI++ +  + A  V++    H  +IR +  L       + F + +S  ++ ++        + D+I       +   P  + F  L   I     + +     +  L++   ADGE+F++ KYRRSYDSDFTAPLV +H+WP L++N  V++KGEA TR
Sbjct:  300 ALLSRFSDAYSQGRLDAQCLLRRCIDKAETVQRIIY-IATVEAFHVAKMAFRH-FKIRVRKSLTPSCAGSNDFEDAVSDYIICHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLSNFIQEICCIAFAMQTLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTR 488

HSP 2 Score: 53.1434 bits (126), Expect = 5.284e-6
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNV 428
            ++L    +      LQ+KL +W  E+  N  + N++ C ELIE  A++Q QLF +L      GG       +K++L+P+L   +    L   V
Sbjct:    3 DNLRRLVSNEALRSLQDKLESWLREYNANSCDQNLNHCLELIEQVAKVQGQLFGILTIAAQEGGHYDGVETIKSRLLPWLEASFTAASLGKPV 95
[Close]
BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A1S3J1D6 (mitochondria-eating protein isoform X3 OS=Lingula unguis OX=7574 GN=LOC106169214 PE=4 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 6.977e-59
Identity = 136/436 (31.19%), Postives = 223/436 (51.15%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNG--LTSNVVMNILNEVGKHTKRPST-KPQNSRNTCTKNNN----GFKEKTLKQGIYDRLDELENIIMDLSNDTXXXXXXXXXXXXXXXXXXXXXFTDNLNDLSTMDANIQSE-----------------PPGDFSGS-----------DVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDLINNRGSYYR-------FQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            ESL    N   F ILQEKL  W  ++  N  + N+++CCE+IELNA++Q Q+FKLL  C   GG+ G    +K++L+P+LG+ +F +G  +T++  +++L E     +  +  + Q  R+     +N      +   L+  + +  DEL+         TK  S  +    +        K+ DN  +LS +   I+S+                 P  D S +             +++   +  Q L+ R+ND+F++ R+ AMD LR Y+DDHENNQRI+FA ++ +        R  + ++R+     H   +T+  +V   I R   +YDL + +    R         +P ++S+  +   I    +L W      + L++  ++D E+FD++KYRRSYDS++TAPLV+HHIW CL Q  +V+VKGEACTR
Sbjct:    3 ESLRRLVNIASFSILQEKLERWLDDYHVNSCDQNVARCCEIIELNAKVQGQIFKLLSLCASEGGMYGGAGAIKSRLLPWLGQSFFASGGSVTADTSLSVLAEAASKDREINELQDQYERSIVDLEDNLRVTNIENDNLRTDLAEAQDELDR--------TKRESTSEKMFNESEIRDLRNKYRDNQVELSRLRGVIESKDAEIRRLRNELILAGGRPGIDMSDTYRSLPRVSSPLSPDDVTQRVRQQNLITRFNDMFAQDRLDAMDILRRYSDDHENNQRIVFAALVEAFASAKLAFRQFKLKVRSNLAATHVGPDTLEEAVQDYINRNTDLYDLPSLQADVVRALNRNIKIFLPADISYNIIGTFIREACKLAWQMSALAHPLDIAVSSDAELFDDYKYRRSYDSEYTAPLVNHHIWACLQQGTKVIVKGEACTR 430
[Close]
BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A1I8IDM0 (MIEAP domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 5.605e-51
Identity = 130/446 (29.15%), Postives = 211/446 (47.31%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGS-EIKLKNQLIPYLGKDYFDNG----------------LTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGIYDRLDELEN--IIMDLSND---TXXXXXXXXXXXXXXXXXXXXXFTDN-LNDLS------TMDANI-QSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQIIYDLINNRGSYY-----------------RFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMI 1346
            + L        F +LQE+L+ W   +KHN  + N++  CELIELNAR+Q+QLFKLL  C   G L G+    ++ +L P LG D+F++                 L+++  ++++   G    R   +P      C      F +    +     LDELE+   +M  +ND    ++   +   +++   S+      DN + +L+      T+D ++ Q  PPG  S   +         Q L+ R+N+ F+ SR  AM+ LR   DDHENNQRI++A V+ S        R  + R+R+           I+R V  ++  + + D IN     Y                 R  +P  + +  +   +    ++ W      + L++    DGE+FD+++YRRSYDS+F+A  VSHHIWP L+Q   +L+KGEACTR  +  +
Sbjct:    3 DCLRKLVTIRSFSLLQERLDRWNQTYKHNSYDENLANACELIELNARVQSQLFKLLSLCAAEGDLLGAHPATIRARLQPLLGPDFFESAAADQADDEDDDVPAGCLSADAALSVIANAG--AARSHQQP-----ACGAPGRRFVDVDEVKIPGAELDELEHDLRLMRRANDGIRAQLVQARTELERQARVSAGEKMLADNEIRELTARALSDTVDRHLAQRGPPGPQSPLSLSHPWQQSRQQHLIARFNEAFTASRAEAMEALRCCYDDHENNQRIVYAAVVESFNSAKVAFRSFKARVRS---------HLIARRVGPDLLEEAVQDFINCHTDLYDLHTLVQEVLRCLSRNSRLYLPPGIGYDVIAPFVTECCRIAWEMTALAHPLDVAVGYDGELFDDYRYRRSYDSEFSATGVSHHIWPALMQGSGLLMKGEACTRRGHPGV 432
[Close]
BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A095A3H7 (Mitochondria-eating protein OS=Schistosoma haematobium OX=6185 GN=MS3_08599 PE=4 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 2.616e-42
Identity = 131/440 (29.77%), Postives = 194/440 (44.09%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGI--YDRLDELENIIMDLSNDTXXXXXXXXXXXXXXXXXXXXXFTDN-------------LNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQG-----------------LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNK---NELIHQFRETISRSVLKNIRTQIIYD-----LINNRGSYYRFQIP------QNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAA-DGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            ESLN  A +  F IL E L  W   ++ N  + N++  CE+IELNARIQ QLF+L+  C   GG  G    +K +L+P LG     +G            VGK T                N+N F  KT +  I  Y R D+ E  +++   +      + +  + ++       +  N             L +L T     + +  G  S S    +   + L G                 LV R+N+LFS  R+ AM  LR Y DD+E +Q+I+F  V+ S        R ++NR+R +   N LI    ET+   V   I     +      L+ +       ++P      QNL F  ++  +  T +L W      + LE+   A + E+ DE KYRRS+DS +   L+ HHIWPCL+QN R++ KGE CTR
Sbjct:    3 ESLNQLAKSGAFLILHENLERWASSYQTNTCDENVAMGCEIIELNARIQNQLFRLISICASEGGPYGGANAIKLRLLPLLGSGSVFSGFA----------VGKQTS---------------NSNLFSPKTYESDISKYPRQDKYEVDLIETRKENNRLREQILSLEGENERLKTHIYCPNKNEYDFTKDNSGSLKNLPTSSKMYEEKDVGSLSLSAPSNLTPSM-LTGVSPLSPNDPIQRYRQHILVMRFNELFSLHRVEAMGILRRYIDDYEASQKIIFYTVLDSFHVAKSHFRAYKNRVRKQIAANNLITT--ETLDELVQSYINIHASHSADISLLVKDACLALERRVPRIRRPSQNLGFEVIKDFLYETCKLAWECAALSHPLEICRPACENELIDEAKYRRSHDSSYPTLLIHHHIWPCLMQNERIVAKGEVCTR 414
[Close]
BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: G4VGZ7 (MIEAP domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_075140 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 9.727e-42
Identity = 131/440 (29.77%), Postives = 193/440 (43.86%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGI--YDRLDELENIIMDLSNDTXXXXXXXXXXXXXXXXXXXXXFTDNLND-------------LSTMDANIQSEPPGDFSGSDVEEIRSDLYLQG-----------------LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNK---NELIHQFRETISRSVLKNIRTQIIYD-----LINNRGSYYRFQIP------QNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAA-DGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            ESLN  A +  F IL E L  W   ++ N  + N++  CE+IELNARIQ QLF+L+  C   GG  G    +K +L+P LG     +  T          VGK T                N N F  KT +     Y R D+ E  +++   +      + +  + ++       +  N N+             L T     + +  G  S S   ++   + L G                 LV R+N+LFS  R+ AM  LR Y DD+E NQ+I+F  V+ S        R ++NR+R +   N LI    ET+   V   I     +      L+ +       ++P      QNL F  ++  +  T +L W      + LE+   A + E+ DE KYRRS+DS +   L+ HHIWPCL+QN R++ KGE CTR
Sbjct:    3 ESLNQLAKSGAFLILHENLERWASSYQRNSCDENVAMGCEIIELNARIQNQLFRLISICASEGGPYGGANAIKLRLLPLLGSGNVFSSFT----------VGKQTS---------------NANLFPPKTNESDTTKYPRRDKYEFDLIETRKENNRLREQILSLEGENERLKTHIYCPNTNEYDFNKDNSGSLKNLPTSSKTYEEKDIGPLSLSAPSDLTPSM-LTGVSPLSPNDPIQRYRQHILVMRFNELFSLHRVEAMGILRRYIDDYEANQKIIFYTVLDSFHVAKSHFRAYKNRVRKQIAANNLITT--ETLDELVQSYINIHASHSADISLLVKDTCLALERRVPRIRRPSQNLGFEVIKDFLYETCKLAWECAALSHPLEICRPACENELIDEAKYRRSHDSSYPTLLIHHHIWPCLMQNDRIVAKGEVCTR 414
[Close]
BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A3Q0KHV8 (MIEAP domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.488e-41
Identity = 131/440 (29.77%), Postives = 193/440 (43.86%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGI--YDRLDELENIIMDLSNDTXXXXXXXXXXXXXXXXXXXXXFTDNLND-------------LSTMDANIQSEPPGDFSGSDVEEIRSDLYLQG-----------------LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNK---NELIHQFRETISRSVLKNIRTQIIYD-----LINNRGSYYRFQIP------QNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAA-DGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            ESLN  A +  F IL E L  W   ++ N  + N++  CE+IELNARIQ QLF+L+  C   GG  G    +K +L+P LG     +  T          VGK T                N N F  KT +     Y R D+ E  +++   +      + +  + ++       +  N N+             L T     + +  G  S S   ++   + L G                 LV R+N+LFS  R+ AM  LR Y DD+E NQ+I+F  V+ S        R ++NR+R +   N LI    ET+   V   I     +      L+ +       ++P      QNL F  ++  +  T +L W      + LE+   A + E+ DE KYRRS+DS +   L+ HHIWPCL+QN R++ KGE CTR
Sbjct:    3 ESLNQLAKSGAFLILHENLERWASSYQRNSCDENVAMGCEIIELNARIQNQLFRLISICASEGGPYGGANAIKLRLLPLLGSGNVFSSFT----------VGKQTS---------------NANLFPPKTNESDTTKYPRRDKYEFDLIETRKENNRLREQILSLEGENERLKTHIYCPNTNEYDFNKDNSGSLKNLPTSSKTYEEKDIGPLSLSAPSDLTPSM-LTGVSPLSPNDPIQRYRQHILVMRFNELFSLHRVEAMGILRRYIDDYEANQKIIFYTVLDSFHVAKSHFRAYKNRVRKQIAANNLITT--ETLDELVQSYINIHASHSADISLLVKDTCLALERRVPRIRRPSQNLGFEVIKDFLYETCKLAWECAALSHPLEICRPACENELIDEAKYRRSHDSSYPTLLIHHHIWPCLMQNDRIVAKGEVCTR 414
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:103047636])

HSP 1 Score: 119.783 bits (299), Expect = 1.729e-28
Identity = 66/189 (34.92%), Postives = 102/189 (53.97%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSV---------LKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            LV R++DL++  R+ A   LR Y DD E  QRI+F   + S +      R  + R+R      H   ET+  +V         L +++T +  D+IN+     R   P  + FV +   I    ++ +        L+L  ++DGE++ + KYRRSYDS+FTAPLV++H+WP L++   V+VKGEA TR
Sbjct:  260 LVSRFSDLYATERLEAQSFLRRYIDDLETVQRIIFIAAVESFQAAKLAYRQFKLRVRKTLSPTHVGPETLEDAVVDYIVRNLDLYDVQTSV-NDVINSMNVNPRISFPPEVDFVLISSFIREMCRIAFAMQTLDPTLDLAFSSDGELYSDMKYRRSYDSEFTAPLVAYHVWPALMEGDAVIVKGEAVTR 447

HSP 2 Score: 66.2402 bits (160), Expect = 3.782e-11
Identity = 28/81 (34.57%), Postives = 48/81 (59.26%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L    NT+ + +LQ+KL  W  ++     + N+++CCEL+EL ++IQ QLF +L      GG       LK++L+P+L 
Sbjct:    3 DTLRRLVNTSSYSVLQQKLEAWYKDYHVISCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYAGADTLKSRLLPWLS 83
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:103047636])

HSP 1 Score: 119.398 bits (298), Expect = 2.263e-28
Identity = 66/189 (34.92%), Postives = 102/189 (53.97%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSV---------LKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            LV R++DL++  R+ A   LR Y DD E  QRI+F   + S +      R  + R+R      H   ET+  +V         L +++T +  D+IN+     R   P  + FV +   I    ++ +        L+L  ++DGE++ + KYRRSYDS+FTAPLV++H+WP L++   V+VKGEA TR
Sbjct:  260 LVSRFSDLYATERLEAQSFLRRYIDDLETVQRIIFIAAVESFQAAKLAYRQFKLRVRKTLSPTHVGPETLEDAVVDYIVRNLDLYDVQTSV-NDVINSMNVNPRISFPPEVDFVLISSFIREMCRIAFAMQTLDPTLDLAFSSDGELYSDMKYRRSYDSEFTAPLVAYHVWPALMEGDAVIVKGEAVTR 447

HSP 2 Score: 66.2402 bits (160), Expect = 4.407e-11
Identity = 28/81 (34.57%), Postives = 48/81 (59.26%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLG 392
            ++L    NT+ + +LQ+KL  W  ++     + N+++CCEL+EL ++IQ QLF +L      GG       LK++L+P+L 
Sbjct:    3 DTLRRLVNTSSYSVLQQKLEAWYKDYHVISCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYAGADTLKSRLLPWLS 83
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:497395])

HSP 1 Score: 69.707 bits (169), Expect = 4.913e-13
Identity = 28/84 (33.33%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDY 401
            ESL   AN+  +  LQ++L++W+ +F  N    N+S+CCELIE+ +++Q QLF ++      GG       +K++ +P+L   +
Sbjct:    3 ESLRRLANSGAYGALQDRLDSWQDDFHLNTCNENLSRCCELIEMTSKVQNQLFNIMSSTANRGGPTAGAETIKSRFLPWLSSSF 86
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Nematostella
Match: EDO49651 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFS0])

HSP 1 Score: 80.8777 bits (198), Expect = 3.334e-16
Identity = 56/198 (28.28%), Postives = 101/198 (51.01%), Query Frame = 3 
Query:  789 LVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNK-----NELI--HQFRETISRSVLKNIRTQIIYDLINN--RGSYYRFQIPQNLSFVE--LEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR--SSNKM 1343
            LV R+ +L+   R+  MD LR   D  EN +R+ F +V          QR  R ++R+        LI   Q  E +   + +N     +  LI    +  +    + Q++ + E  +   I   +++ W+    Q  +++  A DG++ +E +YRRSY+S+F+A +V H +WP L+++ +V+ KGE  T+  S+NK+
Sbjct:  233 LVERFAELYGGERLELMDALRPMGDRAEN-ERLSFCIVEECFMVCKTEQRRMRKKVRHALQPPNGSLIDASQTAEIVDSYMSRNTENFDVETLIPEILKCLHRNASVNQSIPYKEKLILPFIRACVRVVWSMVALQPPIDIAIALDGDIINEARYRRSYNSEFSATVVEHFVWPALMRDGKVVAKGEVVTKRISANKL 429

HSP 2 Score: 52.7582 bits (125), Expect = 2.861e-7
Identity = 28/67 (41.79%), Postives = 38/67 (56.72%), Query Frame = 3 
Query:  189 ILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYL 389
            +L E+L     E     T+ + + CCE+IE N RIQ +LF+LL  C   GG  G    LKN+L+P L
Sbjct:   20 VLHERLITLFDESYRYTTDESTTVCCEIIEENLRIQAKLFRLLQLCCDEGGTYGGFQPLKNKLMPLL 86
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:101174412])

HSP 1 Score: 111.694 bits (278), Expect = 9.779e-26
Identity = 74/221 (33.48%), Postives = 113/221 (51.13%), Query Frame = 3 
Query:  684 LNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-----INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L  LS   + ++SE P     SD           GLV R++ L++  R+ A + LR Y  D E  Q+I+F  V+ S +      R  + R++      H   E++  + +  I R   +YD+     IN      +   P  + F  +  LI  T +L +        L+L  A+DGE+F++ KYRRS+DSDFTAPLV+ H+WP L++ + V+VKGEA TR
Sbjct:  228 LPRLSRPSSPLRSESPTRAQLSDSSR------RAGLVSRFSGLYAVERLEAQNLLRRYITDLEMVQKIIFIAVVESFRSAKLAYRQFKLRVKTTLSSSHFGPESLEDAAVDYIVRNLDLYDVQNNDVINAMNVNPQISFPPEVDFSLISALIRETCRLAFAMQTLDPPLDLAFASDGELFNDTKYRRSFDSDFTAPLVTFHVWPALMEGNGVIVKGEAVTR 442

HSP 2 Score: 75.8702 bits (185), Expect = 3.707e-14
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDY 401
            ESL     T  F +LQ+KL +W  +++    + N+++CCELIEL A++Q+QLF +L      GG       LK +L+P+LG  +
Sbjct:    3 ESLRRLTQTASFRVLQDKLESWHRDYRTISCDQNLNRCCELIELTAKLQSQLFAILNLAAAEGGHYAGVDTLKTRLLPWLGTSF 86
[Close]
BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:101174412])

HSP 1 Score: 111.694 bits (278), Expect = 9.940e-26
Identity = 74/221 (33.48%), Postives = 113/221 (51.13%), Query Frame = 3 
Query:  684 LNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNI-RTQIIYDL-----INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTR 1328
            L  LS   + ++SE P     SD           GLV R++ L++  R+ A + LR Y  D E  Q+I+F  V+ S +      R  + R++      H   E++  + +  I R   +YD+     IN      +   P  + F  +  LI  T +L +        L+L  A+DGE+F++ KYRRS+DSDFTAPLV+ H+WP L++ + V+VKGEA TR
Sbjct:  228 LPRLSRPSSPLRSESPTRAQLSDSSR------RAGLVSRFSGLYAVERLEAQNLLRRYITDLEMVQKIIFIAVVESFRSAKLAYRQFKLRVKTTLSSSHFGPESLEDAAVDYIVRNLDLYDVQNNDVINAMNVNPQISFPPEVDFSLISALIRETCRLAFAMQTLDPPLDLAFASDGELFNDTKYRRSFDSDFTAPLVTFHVWPALMEGNGVIVKGEAVTR 442

HSP 2 Score: 76.2554 bits (186), Expect = 2.831e-14
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDY 401
            ESL     T  F +LQ+KL +W  +++    + N+++CCELIEL A++Q+QLF +L      GG       LK +L+P+LG  +
Sbjct:    3 ESLRRLTQTASFRVLQDKLESWHRDYRTISCDQNLNRCCELIELTAKLQSQLFAILNLAAAEGGHYAGVDTLKTRLLPWLGTSF 86
[Close]
BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Match: SMESG000057251.1 (SMESG000057251.1)

HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0
Identity = 406/410 (99.02%), Postives = 407/410 (99.27%), Query Frame = 3 
Query:  147 MESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNEVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGIYDRLDELENIIMDLSNDTXXXXXXXXXXXXXXXXXXXXXFTDNLNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMICSVQVRNRNV 1376
            MESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILN+V KHTKRPST PQNSRNTCTKNNNGFKEKTLKQGIYDRLDELENIIMDLSNDTKVSSPKQIKKKKKHRSSSLPKFTDNLNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMICSVQ RNRNV
Sbjct:    1 MESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNDVKKHTKRPSTNPQNSRNTCTKNNNGFKEKTLKQGIYDRLDELENIIMDLSNDTKVSSPKQIKKKKKHRSSSLPKFTDNLNDLSTMDANIQSEPPGDFSGSDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQIIYDLINNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMICSVQARNRNV 410
[Close]
BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Match: SMESG000019081.1 (SMESG000019081.1)

HSP 1 Score: 120.939 bits (302), Expect = 6.622e-29
Identity = 73/204 (35.78%), Postives = 112/204 (54.90%), Query Frame = 3 
Query:  783 QGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELI-HQFRETISRSVLKNIRTQIIYDLINNRGS-----YYRFQIP-----QNLSFVELEGLINNTIQLFWNFGGAQYNLELLNA-ADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMICSVQ 1358
            Q LV + NDLFS SR+ AM  L  Y+DDHENNQ+I+F  VM S        R +++R+R   +LI H    ++  SV   I T    D+  N  S        F++P       LSF  +   I    ++ W        +++ +  ++GE++DE +YRRSYDS+++A  +SHH+WPCL ++  V+ KGEA T+ S  +  S++
Sbjct:  256 QQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRT--DLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVTKRSGSLTKSLR 457

HSP 2 Score: 99.3673 bits (246), Expect = 9.011e-22
Identity = 44/106 (41.51%), Postives = 66/106 (62.26%), Query Frame = 3 
Query:  150 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNEVGKHTK 467
            E L    NT  F ILQ +L +W   +K+N  + N+++ CE+IELNA++Q QLFKLL  C   GG+ G    +KN+L+P LGK +F  GL+S+  + +L +   + K
Sbjct:    3 EPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDAASNKK 108
The following BLAST results are available for this feature:
BLAST of MIEAP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
SPATA182.887e-1830.89spermatogenesis associated 18 [Source:HGNC Symbol;... [more]
SPATA183.083e-1830.89spermatogenesis associated 18 [Source:HGNC Symbol;... [more]
SPATA183.576e-1830.89spermatogenesis associated 18 [Source:HGNC Symbol;... [more]
SPATA183.959e-832.26spermatogenesis associated 18 [Source:HGNC Symbol;... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MIEAP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MIEAP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spata183.632e-2533.33spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSXETT00000051452.17.189e-2533.51spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
ENSXETT00000051448.19.070e-2533.51spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
itga82.637e-2230.39integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GE... [more]
itga83.223e-2231.38integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Spata184.618e-2130.73spermatogenesis associated 18 [Source:MGI Symbol;A... [more]
Spata181.233e-1929.78spermatogenesis associated 18 [Source:MGI Symbol;A... [more]
Spata181.362e-1929.78spermatogenesis associated 18 [Source:MGI Symbol;A... [more]
Spata181.898e-1929.78spermatogenesis associated 18 [Source:MGI Symbol;A... [more]
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BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q503Q1|MIEAP_DANRE2.742e-2433.33Mitochondria-eating protein OS=Danio rerio OX=7955... [more]
sp|Q498J5|MIEAP_XENLA2.569e-2131.38Mitochondria-eating protein OS=Xenopus laevis OX=8... [more]
sp|Q6AYL6|MIEAP_RAT2.812e-1930.69Mitochondria-eating protein OS=Rattus norvegicus O... [more]
sp|E1BW58|MIEAP_CHICK4.204e-1931.91Mitochondria-eating protein OS=Gallus gallus OX=90... [more]
sp|E1BLK7|MIEAP_BOVIN7.447e-1931.41Mitochondria-eating protein OS=Bos taurus OX=9913 ... [more]
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BLAST of MIEAP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3J1D66.977e-5931.19mitochondria-eating protein isoform X3 OS=Lingula ... [more]
A0A1I8IDM05.605e-5129.15MIEAP domain-containing protein OS=Macrostomum lig... [more]
A0A095A3H72.616e-4229.77Mitochondria-eating protein OS=Schistosoma haemato... [more]
G4VGZ79.727e-4229.77MIEAP domain-containing protein OS=Schistosoma man... [more]
A0A3Q0KHV81.488e-4129.77MIEAP domain-containing protein OS=Schistosoma man... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
spata181.729e-2834.92spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
spata182.263e-2834.92spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spata184.913e-1333.33spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MIEAP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO496513.334e-1628.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
spata189.779e-2633.48spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
spata189.940e-2633.48spermatogenesis associated 18 [Source:NCBI gene;Ac... [more]
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BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000057251.10.000e+099.02SMESG000057251.1[more]
SMESG000019081.16.622e-2935.78SMESG000019081.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026086 ID=SMED30026086|Name=MIEAP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1760bp
TTTACATAACGTAGAATTTTAAAATCATTTTTAGATAATATATTATAGCT
TTATTTTAAAATTAAATTATTTTATTATTTTAATATCTATAACAAATTAT
TTTTTATTTATTATAAAATAAATCTCTGACAAAATTAATTCAACAAATGG
AAAGTTTAAATTCTTTTGCTAATACAAATTGTTTCTATATTCTTCAAGAA
AAGCTCAATAATTGGAAGCATGAATTCAAACATAATTGTACTGAAGCCAA
CATTAGTAAATGCTGTGAATTAATAGAATTAAATGCCCGAATACAAACTC
AATTATTCAAATTATTAGGATTTTGTGGCATATATGGAGGATTAGAAGGC
TCAGAAATCAAATTGAAAAATCAGCTGATACCATATCTAGGAAAAGATTA
TTTTGACAATGGATTGACTTCAAATGTGGTGATGAATATCTTAAATGAGG
TTGGGAAACACACAAAACGACCTTCAACAAAACCACAAAATTCTCGAAAT
ACTTGCACAAAAAACAACAATGGATTCAAAGAAAAAACCCTTAAACAAGG
AATTTATGATCGACTTGATGAACTTGAAAATATCATTATGGATCTGTCAA
ATGACACTAAAGTGAGTTCACCGAAACAAATAAAAAAAAAGAAAAAACAT
CGATCAAGCTCTCTACCAAAATTTACGGATAATTTAAATGATTTGTCTAC
AATGGACGCAAATATCCAATCCGAACCTCCAGGGGACTTTAGTGGAAGTG
ATGTAGAGGAGATTCGAAGTGATCTATATCTTCAAGGCTTGGTTGGTAGA
TATAACGACTTATTCAGTGAGTCTAGAATTACCGCAATGGACCATTTGAG
ACTTTACAATGACGACCATGAAAACAATCAAAGAATATTATTTGCTCTTG
TTATGATTTCAGCAAAATGCGTCAGTGAATTTCAAAGATGCCACCGAAAC
AGAATCAGAAACAAAAATGAACTTATCCATCAATTTCGTGAAACAATTTC
AAGATCTGTTTTGAAAAATATTCGTACACAAATTATTTATGATTTAATTA
ATAACAGAGGTAGTTATTATCGGTTCCAAATACCGCAGAATCTTTCATTT
GTCGAGCTTGAAGGCCTTATCAACAATACCATTCAATTATTCTGGAATTT
TGGAGGAGCTCAATATAATTTGGAATTGTTGAATGCTGCCGATGGAGAAA
TGTTTGATGAACACAAATATCGGCGAAGCTATGATTCTGATTTTACGGCC
CCTCTTGTCAGTCACCATATTTGGCCATGTTTAATTCAAAATCACCGAGT
TCTCGTCAAAGGAGAGGCATGTACAAGATCCAGTAATAAAATGATCTGCT
CAGTTCAAGTTCGCAACCGAAATGTTTGTTCAGCCAACAACTCGATCTCT
AGTCGATGCTCATCAAAGTAACGCAGAAAAGCCCGTTGCGTTGTATTTGA
AATATAGATGTTTTAATTTCCGTTTTCCTAATCAAGAAATATTTTCTCTT
TCATATTCAATAATGACAGTCGTTGCGTCGAGTTATATATAGTTGAGAAA
AACAAAAAAAATTATATTCTTCGTTTGTTTACTTGCGAACCTCAGTTGAA
ATCGTGCGCTGAATTACTGTAATTGATGAACTCTGTTTTATTCATAAAGA
GATCTTTTCATTTACATAAATAGCAAATAACATCTAATTTATTTATCTGG
CTTGATATTAATGTCGTTGTATATTGGCATTAGTAAATATATTGTTCTAT
ACAGCCTAAA
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protein sequence of SMED30026086-orf-1

>SMED30026086-orf-1 ID=SMED30026086-orf-1|Name=SMED30026086-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=425bp
MESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQ
TQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILN
EVGKHTKRPSTKPQNSRNTCTKNNNGFKEKTLKQGIYDRLDELENIIMDL
SNDTKVSSPKQIKKKKKHRSSSLPKFTDNLNDLSTMDANIQSEPPGDFSG
SDVEEIRSDLYLQGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFA
LVMISAKCVSEFQRCHRNRIRNKNELIHQFRETISRSVLKNIRTQIIYDL
INNRGSYYRFQIPQNLSFVELEGLINNTIQLFWNFGGAQYNLELLNAADG
EMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACTRSSNKMI
CSVQVRNRNVCSANNSISSRCSSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0035694mitochondrial protein catabolic process
GO:0033554cellular response to stress
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR026169MIEAP
IPR031981MIEAP_C
Vocabulary: cellular component
TermDefinition
GO:0005741mitochondrial outer membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 133..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 157..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 104..125
NoneNo IPR availablePANTHERPTHR21771:SF0MITOCHONDRIA-EATING PROTEINcoord: 2..102
coord: 137..418
IPR031981Mitochondria-eating protein, C-terminal domainPFAMPF16026MIEAPcoord: 215..391
e-value: 1.5E-24
score: 87.3
IPR026169Mitochondria-eating proteinPANTHERPTHR21771FAMILY NOT NAMEDcoord: 2..102
coord: 137..418