TNF receptor-associated factor

Overview
NameTNF receptor-associated factor
Smed IDSMED30026063
Length (bp)1262
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TNF receptor-associated factor (SMED30026063) t-SNE clustered cells

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30026063) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TNF receptor-associated factor (SMED30026063) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30026063) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026063

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30026063SMESG000044583.1 dd_Smed_v4_11100_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30026063SMESG000044583.1 dd_Smed_v4_11100_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30026063SMESG000044583.1 dd_Smed_v4_11100_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ovarySMED30026063SMESG000044583.1 SMED30026063smed_20140614PMID:279364
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30026063SMESG000044583.1 SMED30026063smed_20140614PMID:279364
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
cement glandSMED30026063SMESG000044583.1 SMED30026063smed_20140614PMID:279364
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF2 (TNF receptor associated factor 2 [Source:HGNC Symbol;Acc:HGNC:12032])

HSP 1 Score: 87.0409 bits (214), Expect = 8.081e-18
Identity = 49/147 (33.33%), Postives = 76/147 (51.70%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPI-GCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G FI+ I     K Q A+ G +  I S  FY S  GY+M +++YL GDG      LS++ +++KG  D  LRWPFN+KV +    L  QN    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:  352 GVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLM---LLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 495

HSP 2 Score: 49.6766 bits (117), Expect = 6.793e-6
Identity = 30/102 (29.41%), Postives = 46/102 (45.10%), Query Frame = 1 
Query:  175 FESFKENHNTFVFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIV--------DRAIEKELNKQIIKCPKSEC 456
             E  +   +  + G+  E + Y CS C+N+L+ P Q +CG R+CS CL   L +    C  C +  I +E I +        D A  +E+      CP   C
Sbjct:   12 LELLQPGFSKTLLGTKLEAK-YLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGC 112
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF5 (TNF receptor associated factor 5 [Source:HGNC Symbol;Acc:HGNC:12035])

HSP 1 Score: 83.5741 bits (205), Expect = 1.245e-16
Identity = 50/148 (33.78%), Postives = 77/148 (52.03%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A+DG    I S  FY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q    N++ T  P+ N + + RP G  N   G P F+  S L    N +I D  L L+  
Sbjct:  404 GKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVA 548
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF5 (TNF receptor associated factor 5 [Source:HGNC Symbol;Acc:HGNC:12035])

HSP 1 Score: 83.5741 bits (205), Expect = 1.245e-16
Identity = 50/148 (33.78%), Postives = 77/148 (52.03%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A+DG    I S  FY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q    N++ T  P+ N + + RP G  N   G P F+  S L    N +I D  L L+  
Sbjct:  404 GKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVA 548
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF5 (TNF receptor associated factor 5 [Source:HGNC Symbol;Acc:HGNC:12035])

HSP 1 Score: 83.5741 bits (205), Expect = 1.245e-16
Identity = 50/148 (33.78%), Postives = 77/148 (52.03%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A+DG    I S  FY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q    N++ T  P+ N + + RP G  N   G P F+  S L    N +I D  L L+  
Sbjct:  404 GKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVA 548
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF1 (TNF receptor associated factor 1 [Source:HGNC Symbol;Acc:HGNC:12031])

HSP 1 Score: 76.2554 bits (186), Expect = 7.730e-15
Identity = 63/235 (26.81%), Postives = 106/235 (45.11%), Query Frame = 1 
Query:  487 HHLSKFHLEKPEHLKSPENRPAKFYENIPSTF-----CQHLAHSTN----QIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSL 1149
              L+  H  K + L   E +  + +ENI +         HLA +T+    Q+   +  S+E+   + Q +  + D       +LE +L+ +EE         S  G F++ I  V  +   +  G    + S  FY +  GY++ +++YL GDG   +  LS++ ++++G++D  L WPF  KV      L  QN     I    P+ + A + RP    N   G P F P+S L
Sbjct:   52 EELALQHFMKEKLLAELEGK-LRVFENIVAVLNKEVEASHLALATSIHQSQLDRERILSLEQRVVELQQTLAQKDQALG---KLEQSLRLMEEA--------SFDGTFLWKITNVTRRCHESACGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGTGKRTHLSLFIVIMRGEYDALLPWPFRNKVTF---MLLDQNNREHAIDAFRPDLSSASFQRPQSETNVASGCPLFFPLSKL 271
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Match: trf-1 (TNF Receptor Associated Factor (TRAF) homolog; TRF-1 [Source:UniProtKB/TrEMBL;Acc:G5EG80])

HSP 1 Score: 73.9442 bits (180), Expect = 4.203e-14
Identity = 50/139 (35.97%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:  769 GPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ--------NLIVTILPNE---NPACYGRPIG-CNQGFGEPSFIPISSL 1149
            GP Q I+ I K+Q++   A  G    I SVPF     GY+M+    L GDG+    S+S+Y +L+KG+FD  L WPF++ + IS   L  Q        N+   I P +   N     RP G  N  FG  SF  ++ L
Sbjct:  262 GP-QLIWKIDKLQQRTNEAKSGADTTIFSVPFMSHRFGYKMMACACLFGDGSSAGKSISLYVLLLKGEFDPTLEWPFHRAIKIS---LLDQNPRPEDRVNITYVIDPRKLKANEKFLARPRGERNAAFGSQSFCSLAIL 396
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Match: trf-2 (TNF Receptor Associated Factor (TRAF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N586])

HSP 1 Score: 62.003 bits (149), Expect = 1.908e-10
Identity = 46/142 (32.39%), Postives = 65/142 (45.77%), Query Frame = 1 
Query:  778 QFIFHIPKVQEKIQNAL-DGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLIV-TILPNENPAC---YGRPIGCNQG-FGEPSFIPISSLMLNNKFIIDGQL 1185
            Q  F IPK+   +  A  +     I S PFY    GY+M+      GDG   +   SV+  L+KG++D  L WPF   V  S      + L+  TI  NE P       RP G   G FG  +F+P++ +    +F  DG +
Sbjct:  159 QHTFRIPKIGLTVVKATKNKSHRSIYSQPFYSHGYGYKMMAVAAPYGDGLAFREYFSVFVCLMKGEWDDILEWPFRCDVTFSILSDDKKELLTKTIYVNEMPEIQEFLERPEGLRNGTFGFQNFLPLAKV---TEFAADGDI 297
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0334401)

HSP 1 Score: 59.3066 bits (142), Expect = 2.864e-9
Identity = 40/144 (27.78%), Postives = 67/144 (46.53%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ-------NLIVTILPNENPACYGRPIG--CNQGFGEPSFIPISSLMLNNKFIIDG 1179
            G  ++ I     K+  A      E+ S PFY S  GY++   ++L G+G      +SVY  ++ G++D  L+WPF+  +  +   LF Q        +  + +P+     + RP       GFG P F  IS  +L+++  I G
Sbjct:  322 GTLLWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFT---LFEQGAQSGQGGVAESFVPDPTWENFQRPSNEPDQLGFGFPRF--ISHELLHSRPFIKG 460
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0077429)

HSP 1 Score: 58.9214 bits (141), Expect = 3.658e-9
Identity = 40/144 (27.78%), Postives = 67/144 (46.53%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ-------NLIVTILPNENPACYGRPIG--CNQGFGEPSFIPISSLMLNNKFIIDG 1179
            G  ++ I     K+  A      E+ S PFY S  GY++   ++L G+G      +SVY  ++ G++D  L+WPF+  +  +   LF Q        +  + +P+     + RP       GFG P F  IS  +L+++  I G
Sbjct:  331 GTLLWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFT---LFEQGAQSGQGGVAESFVPDPTWENFQRPSNEPDQLGFGFPRF--ISHELLHSRPFIKG 469
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf6 (gene:FBgn0265464 transcript:FBtr0071188)

HSP 1 Score: 48.1358 bits (113), Expect = 9.444e-6
Identity = 74/342 (21.64%), Postives = 131/342 (38.30%), Query Frame = 1 
Query:  181 SFKENHNTFVFGSSSEIQQ--------YFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSE--CQAVCEFRQLIHHLSK---FHLEKPEHLKSP--------ENRPA--KFYENIPSTFCQHL----------AHSTNQIREPKS---------------------NSIEKLKQKSQISFGKF----DDVGNQPTRLEST-----LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVI 1017
            +  +NH+ +  GS +  +Q        Y C+IC + L  P  T CG RFC SCL   ++  +  CP        + +I  D    +E+ +    CP S   C  V    +L  HL        ++P+  K P          RP   +  E++ +    H+          A +T Q  +P +                     N ++  +Q  Q  + +          Q TRLE+      L R +  +D  + +    G  ++ I ++   +        N++ S   Y S  GY+   ++  I     H +SL V+  L++ + D+ L WPF  ++ +
Sbjct:   76 ALNQNHH-YAPGSDTSGEQEEELLDSRYECAICIDWLNEPVLTSCGHRFCRSCLTAWMQKNNQCCPMDNKRLSAEHDIFPDNYTRREIEQLKRDCPNSSLGCSVVASPIELHRHLPSCPYRRQQEPQEEKCPFAKIKCDFVGRPETNQLEEHLKADMPHHMQLMLQAFQQTAIATWQPHKPSTSGAAVENGHGQQQLPPPPQYANGVD--EQIVQTMYQRIVVLEQRTREQETRLENMQKQLRLARQQAPVDPRYSN----GTIVWRIEQLGALVARLRANANNQVYSHECYTSPHGYKFCARLN-IQPRKPHVLSLHVH--LMQSENDYHLDWPFKGRIKL 407
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: BX901915.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:28768979:-1 gene:ENSDARG00000109857.1 transcript:ENSDART00000191629.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX901915.1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.904e-18
Identity = 58/204 (28.43%), Postives = 101/204 (49.51%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-----LKRIEENIDFCWISESG-PGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQL 1185
            N+  E  + ++E   ++ ++   K + + N+  +LE T     L  +E       +S +   G F++ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D  +
Sbjct:   64 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGLRDLSIVEMEAKMREMSAATYDGVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTL---MLLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTI 264
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: AL844899.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28760787:-1 gene:ENSDARG00000111038.1 transcript:ENSDART00000183806.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL844899.1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.904e-18
Identity = 58/204 (28.43%), Postives = 101/204 (49.51%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-----LKRIEENIDFCWISESG-PGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQL 1185
            N+  E  + ++E   ++ ++   K + + N+  +LE T     L  +E       +S +   G F++ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D  +
Sbjct:   64 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGLRDLSIVEMEAKMREMSAATYDGVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTL---MLLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTI 264
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2a (Tnf receptor-associated factor 2a [Source:ZFIN;Acc:ZDB-GENE-071120-2])

HSP 1 Score: 87.0409 bits (214), Expect = 5.255e-18
Identity = 58/209 (27.75%), Postives = 104/209 (49.76%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-----LKRIEENIDFCWISESG-PGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            N+  E  + ++E   ++ ++   K + + N+  +LE T     L  +E       +S +   G F++ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D  + ++ +
Sbjct:  364 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGLRDLSIVEMEAKMREMSAATYDGVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLM---LLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAI 569
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 85.5001 bits (210), Expect = 1.886e-17
Identity = 61/249 (24.50%), Postives = 117/249 (46.99%), Query Frame = 1 
Query:  529 KSPENRPAKFYENIPS----------TFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIE----------ENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + PE+ P     N+            T  +++    N+  E  + ++E + ++ ++   K +++ N+  +LE TL   +            + FC    +  G FI+ I +   + Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ +++KG +D  L+WPF++KV +    L  QN    +I    P+ +   + RPI   N   G P F P++ L   + ++ D  + ++ +
Sbjct:  285 RGPEDAPPAGAHNVGRGGGPGVQKKVTTLENIVCVLNKEVERSALTMEAISRQHRLDQEKIENLSNKVRQLERTLTTRDLQLAESEQSLRELQFC----TYDGVFIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLM---LLDQNNREHIIDAFRPDVSSTSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAI 526
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 85.5001 bits (210), Expect = 1.886e-17
Identity = 61/249 (24.50%), Postives = 117/249 (46.99%), Query Frame = 1 
Query:  529 KSPENRPAKFYENIPS----------TFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIE----------ENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + PE+ P     N+            T  +++    N+  E  + ++E + ++ ++   K +++ N+  +LE TL   +            + FC    +  G FI+ I +   + Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ +++KG +D  L+WPF++KV +    L  QN    +I    P+ +   + RPI   N   G P F P++ L   + ++ D  + ++ +
Sbjct:  285 RGPEDAPPAGAHNVGRGGGPGVQKKVTTLENIVCVLNKEVERSALTMEAISRQHRLDQEKIENLSNKVRQLERTLTTRDLQLAESEQSLRELQFC----TYDGVFIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLM---LLDQNNREHIIDAFRPDVSSTSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAI 526
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 89.3521 bits (220), Expect = 1.150e-18
Identity = 65/237 (27.43%), Postives = 122/237 (51.48%), Query Frame = 1 
Query:  532 SPENRPAKFYENIP--STFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKR-------IEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            S +  PA  ++ +   +T  +++    N+  E  S++ E+L ++ + +  K + +  +  +LE TL +       +E  +    +S +  G FI+ I +  +K Q+A++G    + S PFY +  GY+M +++YL GDG      LS++ +++KG+ D  LRWPFN+KV +    L  QN    +I    P+ + + + RPI   N   G P F  +S L   N ++ D  + ++ +
Sbjct:  243 SVQGSPAPPFDTLDKKNTTFENIVSVLNREVERSSHAAEELARQLRAAQEKVEALTTKVRQLERTLGQKELIVAELETRMQESELS-TYDGVFIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTGRNTHLSLFFVVMKGNNDALLRWPFNQKVTLM---LLDQNNREHVIDAFRPDVSSSSFQRPISEMNIASGCPLFCQVSKLEGKNSYVRDDTIFVKVI 475
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 86.6557 bits (213), Expect = 8.519e-18
Identity = 57/178 (32.02%), Postives = 94/178 (52.81%), Query Frame = 1 
Query:  703 TRLESTLKR-------IEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            TRLE TL +       +E  +    +S +  G FI+ I +  +K Q+A++G    + S PFY +  GY+M +++YL GDG      LS++ +++KG+ D  LRWPFN+KV +    L  QN    +I    P+ + + + RPI   N   G P F  +S L   N ++ D  + ++ +
Sbjct:  275 TRLERTLGQKELIVAELETRMQESELS-TYDGVFIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTGRNTHLSLFFVVMKGNNDALLRWPFNQKVTLM---LLDQNNREHVIDAFRPDVSSSSFQRPISEMNIASGCPLFCQVSKLEGKNSYVRDDTIFVKVI 448
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 72.0182 bits (175), Expect = 4.793e-13
Identity = 44/148 (29.73%), Postives = 75/148 (50.68%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ-----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G  I+ I   + + Q A+ G    + S PFY  + GY+M  +VYL GDG      LS++ ++++G++D  L WPF +KV   S  L  Q     +L     P+ N + + +P G  N   G P F+   +++ N  +I D  + ++ +
Sbjct:  384 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKV---SLMLMDQGPSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVA-QTVLENGTYIKDDTIFIKVI 527
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 72.0182 bits (175), Expect = 5.121e-13
Identity = 44/148 (29.73%), Postives = 75/148 (50.68%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ-----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G  I+ I   + + Q A+ G    + S PFY  + GY+M  +VYL GDG      LS++ ++++G++D  L WPF +KV   S  L  Q     +L     P+ N + + +P G  N   G P F+   +++ N  +I D  + ++ +
Sbjct:  409 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKV---SLMLMDQGPSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVA-QTVLENGTYIKDDTIFIKVI 552
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BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: slc12a1 (solute carrier family 12 member 1 [Source:Xenbase;Acc:XB-GENE-479925])

HSP 1 Score: 68.1662 bits (165), Expect = 9.488e-12
Identity = 49/150 (32.67%), Postives = 73/150 (48.67%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTIL-PNENPACYGRPIG-CNQGFGEPSFIPISSLM--LNNKFIIDGQLQLRFV 1200
            G+ I+ I   + K + A++G    I S  FY S  GYR+  + YL GDG+     LS+Y +++KG+FD  L WPF +KV +    L  Q    N I  +   + N + + RP    N   G P F   + L    N  +I D  L ++ V
Sbjct:  384 GKLIWKITDYERKKREAMEGRAVSIFSQHFYTSWCGYRLCARAYLNGDGSGKGSFLSLYFVVMKGEFDSILLWPFKQKVTLM---LLDQSGKKNHITDVFRADPNSSSFKRPDSEMNIASGCPRFASHAQLENPKNGCYIKDDTLFIKIV 530
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf2 (TNF receptor-associated factor 2 [Source:MGI Symbol;Acc:MGI:101835])

HSP 1 Score: 83.5741 bits (205), Expect = 7.053e-17
Identity = 55/201 (27.36%), Postives = 97/201 (48.26%), Query Frame = 1 
Query:  634 SIEKLKQKSQISFGKFDDVGNQPTRLEST-------LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + E   ++ ++   K + + N+  +LE +       +  +E+ +    +S +  G FI+ I     K Q A+ G    I S  FY S  GY+M ++VYL GDG      LS++ +++KG  D  L+WPFN+KV +    L   N    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:  299 TAEACSRQHRLDQDKIEALSNKVQQLERSIGLKDLAMADLEQKVSELEVS-TYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLM---LLDHNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 495

HSP 2 Score: 49.2914 bits (116), Expect = 6.336e-6
Identity = 31/102 (30.39%), Postives = 45/102 (44.12%), Query Frame = 1 
Query:  175 FESFKENHNTFVFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIV--------DRAIEKELNKQIIKCPKSEC 456
             E  +   +  + G+  E + Y CS CKNIL+ P Q +CG R+CS CL   L +    C  C    + +E I +        D A  +E+      CP   C
Sbjct:   12 LELLQPGFSKTLLGTRLEAK-YLCSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNCAACVYEGLYEEGISILESSSAFPDNAARREVESLPAVCPNDGC 112
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf2 (TNF receptor-associated factor 2 [Source:MGI Symbol;Acc:MGI:101835])

HSP 1 Score: 83.5741 bits (205), Expect = 7.254e-17
Identity = 55/201 (27.36%), Postives = 97/201 (48.26%), Query Frame = 1 
Query:  634 SIEKLKQKSQISFGKFDDVGNQPTRLEST-------LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + E   ++ ++   K + + N+  +LE +       +  +E+ +    +S +  G FI+ I     K Q A+ G    I S  FY S  GY+M ++VYL GDG      LS++ +++KG  D  L+WPFN+KV +    L   N    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:  306 TAEACSRQHRLDQDKIEALSNKVQQLERSIGLKDLAMADLEQKVSELEVS-TYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLM---LLDHNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 502
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 79.7221 bits (195), Expect = 1.982e-16
Identity = 47/149 (31.54%), Postives = 78/149 (52.35%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A++G    + S PFY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q+     ++ T   + N + + RP G  N   G P F+  S+L    N +I D  L L+  
Sbjct:   96 GKLIWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTL---MLLDQSGKKNHIVETFKADPNSSSFKRPDGEMNIASGCPRFVSHSTLENSKNTYIKDDTLFLKVA 241
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 79.7221 bits (195), Expect = 1.982e-16
Identity = 47/149 (31.54%), Postives = 78/149 (52.35%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A++G    + S PFY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q+     ++ T   + N + + RP G  N   G P F+  S+L    N +I D  L L+  
Sbjct:   96 GKLIWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTL---MLLDQSGKKNHIVETFKADPNSSSFKRPDGEMNIASGCPRFVSHSTLENSKNTYIKDDTLFLKVA 241
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 80.8777 bits (198), Expect = 6.028e-16
Identity = 47/149 (31.54%), Postives = 78/149 (52.35%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A++G    + S PFY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q+     ++ T   + N + + RP G  N   G P F+  S+L    N +I D  L L+  
Sbjct:  398 GKLIWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNHIVETFKADPNSSSFKRPDGEMNIASGCPRFVSHSTLENSKNTYIKDDTLFLKVA 543
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|Q12933|TRAF2_HUMAN (TNF receptor-associated factor 2 OS=Homo sapiens OX=9606 GN=TRAF2 PE=1 SV=2)

HSP 1 Score: 87.0409 bits (214), Expect = 3.881e-17
Identity = 49/147 (33.33%), Postives = 76/147 (51.70%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPI-GCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G FI+ I     K Q A+ G +  I S  FY S  GY+M +++YL GDG      LS++ +++KG  D  LRWPFN+KV +    L  QN    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:  352 GVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLM---LLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 495
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|P39429|TRAF2_MOUSE (TNF receptor-associated factor 2 OS=Mus musculus OX=10090 GN=Traf2 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.934e-16
Identity = 55/201 (27.36%), Postives = 97/201 (48.26%), Query Frame = 1 
Query:  634 SIEKLKQKSQISFGKFDDVGNQPTRLEST-------LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + E   ++ ++   K + + N+  +LE +       +  +E+ +    +S +  G FI+ I     K Q A+ G    I S  FY S  GY+M ++VYL GDG      LS++ +++KG  D  L+WPFN+KV +    L   N    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:  299 TAEACSRQHRLDQDKIEALSNKVQQLERSIGLKDLAMADLEQKVSELEVS-TYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLM---LLDHNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 495
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|O00463|TRAF5_HUMAN (TNF receptor-associated factor 5 OS=Homo sapiens OX=9606 GN=TRAF5 PE=1 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 5.977e-16
Identity = 50/148 (33.78%), Postives = 77/148 (52.03%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A+DG    I S  FY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q    N++ T  P+ N + + RP G  N   G P F+  S L    N +I D  L L+  
Sbjct:  404 GKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVA 548
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|P70191|TRAF5_MOUSE (TNF receptor-associated factor 5 OS=Mus musculus OX=10090 GN=Traf5 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.075e-15
Identity = 47/149 (31.54%), Postives = 78/149 (52.35%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLM-LNNKFIIDGQLQLRFV 1200
            G+ I+ +   + K + A++G    + S PFY S  GYR+  + YL GDG+     LS+Y ++++G+FD  L+WPF ++V +    L  Q+     ++ T   + N + + RP G  N   G P F+  S+L    N +I D  L L+  
Sbjct:  404 GKLIWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLM---LLDQSGKKNHIVETFKADPNSSSFKRPDGEMNIASGCPRFVSHSTLENSKNTYIKDDTLFLKVA 549
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|P39428|TRAF1_MOUSE (TNF receptor-associated factor 1 OS=Mus musculus OX=10090 GN=Traf1 PE=1 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 2.222e-13
Identity = 52/177 (29.38%), Postives = 87/177 (49.15%), Query Frame = 1 
Query:  634 SIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSL 1149
            S+E+   + Q +  + D V     +LE +L+ +EE         S  G F++ I  V ++   ++ G    + S  FY +  GY++ +++YL GDG+  K  LS++ ++++G++D  L WPF  KV      L  QN     I    P+ + A + RP    N   G P F P+S L
Sbjct:  224 SLEQRVVELQQTLAQKDQVLG---KLEHSLRLMEEA--------SFDGTFLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTF---MLLDQNNREHAIDAFRPDLSSASFQRPQSETNVASGCPLFFPLSKL 386
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A2I0LIG5 (TNF receptor-associated factor 2 OS=Columba livia OX=8932 GN=TRAF2 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 4.599e-26
Identity = 98/345 (28.41%), Postives = 154/345 (44.64%), Query Frame = 1 
Query:  208 VFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIV--------DRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIR-EPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + G+  E++ Y CS CKNIL+ P Q +CG R+CS CL + + A    C +C    I +E I +        D A  +E+      C  S C     ++  I          PE   + E    K       T      H  +Q + E  SN + +L++    S G  D    +   +E  ++ +E        + +  G FI+ I +   K Q A+ G    I S  FY S  GY+M ++VYL GDG      LS++ +++KG  D  LRWPFN+KV +    L  QN    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:   23 ILGTKLEVK-YLCSDCKNILRRPFQAQCGHRYCSYCLKKIISAGPQKCASCIQEGIYEEGISILETSSAFPDNAARREVESLPAVCINSGCT----WKGTIKEYEAHDEVCPEFPLTCEGCGKKVPREKVPTEAYSRQHRLDQEKIETLSNKVRQLER----SIGLKDLAMAE---MEEKIRNME--------ASTYDGVFIWKITEFARKRQEAITGRSPAIFSPAFYTSKYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLM---LLDQNNREHIIDAFRPDVTSSSFQRPVTEMNIASGCPLFCPVSVMEAKNSYVRDDAIFIKAI 344
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A384B3Z2 (TNF receptor-associated factor OS=Balaenoptera acutorostrata scammoni OX=310752 GN=TRAF2 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 8.908e-25
Identity = 96/365 (26.30%), Postives = 157/365 (43.01%), Query Frame = 1 
Query:  232 QQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIV--------DRAIEKELNKQIIKCPKSECQ---AVCEFRQLIHHLSKFHL-------------EKPEHLKS--PENRPAKFYENIPSTFCQHLAHSTNQIREPKS----NSIEKLKQKSQISFGKFDDVGNQPTRLEST-------LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
             +Y CS C+N+L+ P Q +CG R+CS CL+  L +    C  C N  I +E + +        D A  +E+      C    C     + E+         F L             EK  HL+   PE   +  +   P       AH     + P +       +  ++K ++   + + + N+  +LE +       +  +E+ +     S +  G FI+ IP    K Q+A+ G    I S  FY S  GY+M ++ YL GDG      LS++ +L+KG  D  LRWPFN+KV +    L  QN    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:   46 DKYLCSACRNVLRRPFQAQCGHRYCSFCLSSILSSGPQNCAACVNEGIYEEGVSILESSSAFPDNAARREVESLPAVCSSEGCTWKGTLKEYESCHEGHCPFMLTECPACKGPVRLGEKGHHLEHECPERSLSCRHCRAPCCLADMKAHHQVCPKFPLTCEGCGKKKITREKHRLDQDRIEALSNKVQQLERSIGLKDLAMADLEQKVHEMEAS-TFDGVFIWKIPDFARKRQDAVAGRTPAIFSPAFYTSRYGYKMCLRAYLNGDGTGRGTHLSLFFVLMKGPHDALLRWPFNQKVTLM---LLDQNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAI 406
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: T1D122 (TRAF-5 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.531e-22
Identity = 67/226 (29.65%), Postives = 115/226 (50.88%), Query Frame = 1 
Query:  613 IREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDF-------------CWISESGPGQ-----------FIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS--SCCLFSQNLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQN 1209
            +RE K    + L++K +    K++++       E+++K ++E +D               + S S P +             + I  +++KIQ A  GG NE+ S  FY+   GY M +KV+L GDG      LS + ++ KG++D  + WPF  +V++S  S  L  ++++V + PN N  CYGRP G CN G G+P  + +S ++  + ++  G L+LR    N
Sbjct:  240 LREQKEEMKKMLREKEEDHRKKYEELREINKDNETSVKELKEKVDHITKYSESLLSELTTYKSNSHPKEPLTDIEASLRDVTWSIKDIRKKIQEAKCGGNNELISECFYVFRPGYNMSLKVFLNGDGRATNKYLSTFFVINKGEYDSQISWPFKSRVLLSILSNNLSKEDIVVRMKPNNNDQCYGRPSGRCNLGAGQPDLVELSEILNKDYYLHHGALKLRLTLIN 465

HSP 2 Score: 67.3958 bits (163), Expect = 2.317e-8
Identity = 32/83 (38.55%), Postives = 48/83 (57.83%), Query Frame = 1 
Query:  244 CSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHH 492
            C ICK I+  P Q++CGCRFCS C+   +      CP C +  I K ++I D+AIEKE+ +  ++CP+  C     +R+   H
Sbjct:   22 CGICKQIMFEPTQSQCGCRFCSKCIEEFISKEKRKCPICTDQNIEKVSLIRDKAIEKEIMRMRVQCPQEGCGENIMYREWGGH 104
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: T1DF65 (TRAF-2 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 3.326e-22
Identity = 67/210 (31.90%), Postives = 113/210 (53.81%), Query Frame = 1 
Query:  586 QHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIE--ENIDFCWISESGP--GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS--SCCLFSQNLIVTILPNENPACYGRPI-GCNQGFGEPSFIPISSLMLNNKFIIDGQLQLR 1194
            Q L+ S +   E  S  I++LK+K      + +D+  +  + +S  +++E   N+  C +SE+    G++ + +  + EK+ +A  G   +I S  FY S+ GYRM +K+Y  GDG+     LSVY +L+KG +D  ++WPFN  + I   +     ++ ++ + PN N  CYGRP    N   G+P F+    L+  + FI DG + LR
Sbjct:  256 QDLSVSVSVQNERNSAEIKELKEKLSSLLTEIEDLKTKLKQFQSMYEKLENERNLAKCSLSETKERYGEYTWKVENIMEKVNDAKKGVNTQILSESFYSSNPGYRMCLKIYPNGDGSAFGNYLSVYFVLMKGKYDDQIQWPFNSIISIRLVNIARRGEDRVLNMRPNINDQCYGRPKEEHNLAAGQPKFVSHLQLVNEDDFIKDGAIILR 465
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A226NFY8 (TNF receptor-associated factor OS=Callipepla squamata OX=9009 GN=ASZ78_011648 PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 9.242e-20
Identity = 107/398 (26.88%), Postives = 169/398 (42.46%), Query Frame = 1 
Query:  208 VFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLN-----------------RHLEAIDNF--CPNCK---NFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHL------EKPEHLKS-----------------PENRPAKFYENIPSTFCQHLAHST----------NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-------LKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            + G+  E++ Y CS C+NIL+ P Q +CG R+CS CL                  R LE  D+   C  CK    FE+V         +E E      K P+ E + + E    ++ L  F L         +HL +                 PE+   K  E +    C  L   T          N+  E  S + E   ++ ++   + + + N+  +LE +       +  +EE I     S +  G FI+ I +   K Q A+ G    I S  FY S  GY+M ++VYL GDG+     LS++ +++KG  D  LRWPFN+KV +    L  QN    +I    P+   + + RP+   N   G P F P+S +   N ++ D  + ++ +
Sbjct:   23 ILGTKLEVK-YLCSDCRNILRRPFQAQCGHRYCSYCLKKIIRYESFILRTAGMLLRDLEFQDHVKNCGKCKVPCRFEVVG----CAEMVENE------KLPEHESRCLAEH---LYMLLSFVLSLKSGSRDLKHLPALPSSQSSSPLLAANSLCPESELFKSLELLGR--CDALEKKTVTFENIVCVLNREVERVSLTAEAYSRQHRLDQEQIETLSNKVRQLERSIGLKDLAMAEMEEKIRNMEAS-TYDGVFIWKITEFARKRQEAITGRSPAIFSPAFYTSKYGYKMCLRVYLNGDGSGRGTHLSLFFVVMKGPNDALLRWPFNQKVTL---MLLDQNNREHIIDAFRPDVTSSSFQRPVTEMNIASGCPLFCPVSVMEAKNSYVRDDAIFIKAI 400
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:103028261])

HSP 1 Score: 86.2705 bits (212), Expect = 8.084e-18
Identity = 55/195 (28.21%), Postives = 97/195 (49.74%), Query Frame = 1 
Query:  637 IEKLKQKSQISFGKFDDVGNQPTRLESTL----------KRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIID 1176
            +E L ++ Q+   K +++ N+  +LE TL          ++    + FC    +  G F++ I     + Q+AL G    + S  FY S  GY+M +++YL GDG      LS++ ++++G +D  L+WPF++KV +    L  QN    +I    P+ +   + RPI   N   G P F P++ L   + ++ D
Sbjct:  330 LEALTRQQQLDQEKIENLSNKVRQLERTLTMRDLQLAESEQALRELQFC----TYDGVFVWKISDFSRRRQDALAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMRGKYDALLKWPFSQKVTLM---LLDQNNREHIIDAFRPDISSTSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRD 517
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 85.8853 bits (211), Expect = 1.104e-17
Identity = 58/209 (27.75%), Postives = 104/209 (49.76%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-----LKRIEENIDFCWISESG-PGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            N+  E  + ++E   ++ ++   K + + N+  +LE T     L  +E       +S +   G F++ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D  + ++ +
Sbjct:  361 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGLRDLSIVEMEGKMREMSAATYDGVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLM---LLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAI 566
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 85.5001 bits (210), Expect = 1.213e-17
Identity = 58/209 (27.75%), Postives = 104/209 (49.76%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLEST-----LKRIEENIDFCWISESG-PGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            N+  E  + ++E   ++ ++   K + + N+  +LE T     L  +E       +S +   G F++ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D  + ++ +
Sbjct:  248 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGLRDLSIVEMEGKMREMSAATYDGVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLM---LLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAI 453
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf1 (TNF receptor-associated factor 1-like [Source:NCBI gene;Acc:103045252])

HSP 1 Score: 75.0998 bits (183), Expect = 3.314e-14
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSL 1149
            G F++ I  V +K++ A  G  +   S  FY S  G+++ +++Y+ GDG      +S++ +++KGD+D  L WPF +KV      L  QN    ++    P+   A + RP+   N   G P F P+S L
Sbjct:  392 GTFLWRICDVSQKLKEAATGQKSSQYSPAFYTSRYGFKVCMRLYMYGDGVGKGTHISLFFVIMKGDYDPLLSWPFKQKVTFF---LIDQNQREHVVDAFRPDLRSASFQRPVSEMNVASGCPLFFPLSKL 518
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf1 (TNF receptor-associated factor 1-like [Source:NCBI gene;Acc:103045252])

HSP 1 Score: 75.0998 bits (183), Expect = 3.410e-14
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSL 1149
            G F++ I  V +K++ A  G  +   S  FY S  G+++ +++Y+ GDG      +S++ +++KGD+D  L WPF +KV      L  QN    ++    P+   A + RP+   N   G P F P+S L
Sbjct:  369 GTFLWRICDVSQKLKEAATGQKSSQYSPAFYTSRYGFKVCMRLYMYGDGVGKGTHISLFFVIMKGDYDPLLSWPFKQKVTFF---LIDQNQREHVVDAFRPDLRSASFQRPVSEMNVASGCPLFFPLSKL 495
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002130.1 (pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 gene:ENSPMAG00000001936.1 transcript:ENSPMAT00000002130.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.8702 bits (185), Expect = 3.055e-15
Identity = 44/148 (29.73%), Postives = 73/148 (49.32%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ-----NLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G+ ++ I     + Q A  G    + S PFY S  GY+M  + YL GDG      LS++ ++++G++D  L WPF ++V +S   L  Q     +L  T  P+     + RP G  N   G P F+   +L   + +++D  + +R V
Sbjct:  268 GKLVWKIRDYARRKQEAASGRTLSLYSQPFYTSPFGYKMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLEWPFRRRVTLS---LLDQGAARRHLSDTFTPDPASGSFRRPDGDMNVASGCPLFVSHVALE-GSSYLVDDTVFVRVV 411
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000074.1 (pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-1 gene:ENSPMAG00000000066.1 transcript:ENSPMAT00000000074.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.0922 bits (170), Expect = 2.830e-13
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFI 1134
            G+ ++ I     + Q+A+ G    + S PFY S  GYRM  + YL GDG      LS++ ++++G++D  L WPF ++V ++   L  Q      L  T  P+   + + RP    N   G P F+
Sbjct:  325 GKLVWKIRDYARRKQDAVSGRTLSLYSQPFYTSPFGYRMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLPWPFRQRVTLT---LLDQGAARRQLSDTFKPDPASSSFRRPTSDMNIASGCPLFV 447
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO40665 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6K0])

HSP 1 Score: 83.5741 bits (205), Expect = 1.074e-17
Identity = 91/346 (26.30%), Postives = 149/346 (43.06%), Query Frame = 1 
Query:  235 QYFCSICKNILKMPRQT-ECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPK--SECQAVCEFRQLIHHLS-KFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIREPKSNSIEKLKQKSQISFG-KFDDVGNQPTRLESTLKRIE----------ENIDFCWISES----------GPGQFIFHIPKVQEKIQNALDGGLNE-ISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKV---VISSCCLFS--QNLIVTILPNENPACYGRPIGCNQ-GFGEPSFIPISSLMLNNKFIID 1176
             Y C IC+   + P QT +CG RFC SCL   L     FCP  +   I ++ +  D+A ++ +    +KCP   ++C+ V E   L  + S  + +    H+++ +  P K         C +    T+  RE  +  I+        +   K+ DVG      ++ +KRIE           ++D   I               G+F++ I    +  Q +      E + S PFY    GY++  + +L G G      LS+Y +++KGD+D  L WPF ++V   +I     F+  QN    +  + N   +GRP      GFG P F+   +L   N F+ D
Sbjct:   39 DYQCPICQLPFRDPVQTRDCGHRFCESCLEPILRKPPAFCPIDRRC-ISRDKVYPDKACKRTVLSLTVKCPNHVTDCEWVGELGNLPSNSSNSYEVLVKVHIEACKRVPIK---------CVNNCGRTDIAREEMAVHIDVDGDCPLATVPCKYSDVG-----CKTKVKRIEMEKHLQDSMASHLDMAHIRIRELEQRQEKICTNGKFLWKISNYTQLFQQSATKKEKEKLCSPPFYTGQYGYKLRAEAFLNGLGQGKGTHLSLYVVIMKGDYDSVLPWPFQQRVDFILIDQDDDFTSRQNKTWRLTCDRNSDYFGRPNKAKSLGFGCPKFVSQETLKSRN-FVRD 368
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO49464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG36])

HSP 1 Score: 70.8626 bits (172), Expect = 2.825e-13
Identity = 41/130 (31.54%), Postives = 63/130 (48.46%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLI----VTILPNENPACYGRPIG-CNQGFGEPSFIPISSL 1149
            G +I+ I +   + Q  + G    I S PFY+   GY+   +VY  GDG      LS++ +L++G+FD  L WPF +KV      L  Q+ +     T  P+   + + RP    N   G P FI  ++L
Sbjct:  378 GTYIWKIDEYTRRYQEGVSGKTPSIYSPPFYVGRYGYKACARVYPNGDGMGKGSHLSLFFVLMRGEFDALLPWPFRQKVTFK---LLDQDRVHDIGDTFRPDPTSSSFKRPTSNMNIASGCPLFISHTNL 504
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO35237 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM27])

HSP 1 Score: 65.4698 bits (158), Expect = 1.356e-11
Identity = 46/168 (27.38%), Postives = 72/168 (42.86%), Query Frame = 1 
Query:  682 DDVGNQPTRLESTLKRIEENIDF-------------CWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ----NLIVTILPNENPACYGRPI-GCNQGFGEPSF 1131
            DD    P +L   L ++E +I                  + S  G F++ I K  E+   A  G    I S PFY    GY++  +++  GDG+     LS++  L++G++D  L WPF + + I+   L  Q    N+  T  P+     + RP    N   G P F
Sbjct:  336 DDSQTNPNKLNRRLSQLESSITIHKTKLFQEDLRIQIQQAISYDGTFLWKIDKFTERYAEAKSGNATSIYSSPFYTHRFGYKLCARLFPNGDGSARDNYLSIFIALMRGEYDSILPWPFQETITIT---LIDQDNGMNISETFDPDPCSISFQRPTRNINVASGCPRF 500
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO32849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STV3])

HSP 1 Score: 65.4698 bits (158), Expect = 1.450e-11
Identity = 63/231 (27.27%), Postives = 98/231 (42.42%), Query Frame = 1 
Query:  550 AKFYENIPSTFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLE---STLKRIEENI-DFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQ--------NLIVTILPN---ENPACYGRP-IGCNQGFGEPSFIPISSLMLNNKFIIDGQLQLR 1194
            AK      S  CQH        +  KS  I  +K    +   + + + N   + E   + +K+++  + D   I+ S   Q I+ I     K++ A  G    + S  F  S  GYR+   V L GDG      +SV+  ++KG FD  L+WPF+ +V      L +Q        ++  +I PN   +N    GRP +  N  FG   FI    +   N FI D  + L+
Sbjct:  230 AKLMCEFESLGCQHRCSREKMAKHMKSEIIGHVKMIFGVVLEQKEKLDNHAQKFEEHETIMKQVDSRVGDLEKIAHS---QIIWRIEDYTRKLKEAKAGNAETLFSPTFTTSKHGYRLCASVCLNGDGKGKGTHMSVFVSVLKGAFDALLKWPFHYRVTFF---LLNQHENPEERKHVKFSIKPNPIPDNEPFLGRPRLEKNASFGGAKFIKHEEIESGN-FIKDDTVFLK 453
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO41243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z7])

HSP 1 Score: 63.5438 bits (153), Expect = 6.335e-11
Identity = 44/173 (25.43%), Postives = 78/173 (45.09%), Query Frame = 1 
Query:  685 DVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS-----SCCLFSQNLIVTILPNENPACYGRP-IGCNQGFGEPSFIPISSLMLNNKFIIDGQL 1185
            D G Q   L+  L R+ E +           +F++ IP   + ++ A  G    + S PF+    GY++   +Y  G  +     +S +  + +G++D  L WPF + + I+        L  +N+  TI+PN +   + RP    N G G  +F+    L   N +++DG L
Sbjct:  297 DSGAQFHELQQGLGRVNELLKM---------RFVWRIPDFADSLRQAKLGLKVRLDSEPFFTEKNGYKLRATLYPNGTMSGKNTHISAFVSINRGEYDGILAWPFTRVIKITLLDQKENVLQRRNVEKTIIPNPSLENFHRPKTVSNNGRGSATFVSHEKLWTEN-YVVDGTL 459

HSP 2 Score: 54.299 bits (129), Expect = 5.105e-8
Identity = 29/88 (32.95%), Postives = 47/88 (53.41%), Query Frame = 1 
Query:  232 QQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNF-CPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHH 492
            ++Y C IC   L++P QT+CG RFC  CL+          CP  +    +K+++ +D+A E+ +   ++KCP   C+   E R L  H
Sbjct:   21 EEYTCRICHLPLRVPVQTKCGHRFCKECLDEASRRKSRAECPMDREPLDLKKDVFLDKAAERTILSFLVKCPCKGCKWEAELRDLEAH 108
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf3 (TNF receptor associated factor 3 [Source:NCBI gene;Acc:101161601])

HSP 1 Score: 92.4337 bits (228), Expect = 5.631e-20
Identity = 99/399 (24.81%), Postives = 161/399 (40.35%), Query Frame = 1 
Query:  235 QYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFE-IVKENIIVDRAIEKELNKQIIKCPKSE---CQAVCEFRQLIHHLS-----------------KFHLEKPEHL----------------KSPENRPAKFYENIPSTF---CQHLAHSTNQIREPK---------------SNSIEKLKQKSQISFGKFDDVGNQPT--------RLESTLKRIEENIDFCWI-------------SESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN-----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQL 1185
            +Y C  C+ +L  PRQTECG RFC SC+N  L   +  CP   + E + K+ I  D    +E+    + C +SE   CQ     +Q+  H++                     E PEHL                K P     K  E +   F   C +   S++  R  K                 S+ +L+ + +   G  D V  + +         LE+ L R ++ +    I             + S  G  I+ I   + + Q+A+ G    + S PFY  + GY+M  +VYL GDG      LS++ ++++G++D  L WPF +KV +    L  Q      L     P+ + + + RP+   N   G P F+   S++ +  +I D  +
Sbjct:   45 KYCCEACRLVLCQPRQTECGHRFCYSCINDILSCPNPVCP--ADMEPLFKDKIFRDVCCHREIMALKVYC-RSEANGCQEQMSLQQIPDHVNVCPFFEVPCPLGKCKERMMRKEIPEHLAWKCKYRESTCEFCKNKMPLTELQKHKETVCPAFPVSCPNHCTSSSLPRSEKLMSSHTEKLLELELELRSLRQLRDEMESLRGTLDTVRTRLSALEQGGRASLETQLNRHDDMLSVHEIRLADMDLRFQVLETASYNGTLIWKIRDYKRRKQDAVAGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVVMRGEYDALLPWPFKQKVTLM---LMDQGPSRKHLGDAFKPDPSSSSFRRPVAEMNIASGCPLFVS-QSVLESGTYIKDDTI 436
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: ENSORLT00000020861.2 (TNF receptor-associated factor 2 [Source:NCBI gene;Acc:101167626])

HSP 1 Score: 85.1149 bits (209), Expect = 2.288e-17
Identity = 48/139 (34.53%), Postives = 74/139 (53.24%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIID 1176
            G FI+ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  QN    +I    P+ + + + RP+   N   G P F P+S L   N +I D
Sbjct:  420 GIFIWKISDFTKKRQDAMAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLM---LLDQNNREHIIDAFRPDISSSSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRD 555
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: unm_sa808 (TNF receptor-associated factor 2 [Source:NCBI gene;Acc:101155367])

HSP 1 Score: 83.1889 bits (204), Expect = 8.679e-17
Identity = 47/147 (31.97%), Postives = 77/147 (52.38%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            G FI+ I    +K Q+A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++G  D  L+WPFN+KV +    L  Q+    +I    P+   + + RP+   N   G P F P+S L   N +I D  + ++ +
Sbjct:  447 GVFIWRISDFAKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGLSDALLKWPFNQKVTLM---LLDQSNREHIIDAFRPDVTSSSFQRPVSEMNIASGCPLFCPLSKLDAKNSYIRDDTIFIKAI 590
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 77.411 bits (189), Expect = 6.315e-15
Identity = 51/213 (23.94%), Postives = 102/213 (47.89%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTL----------KRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            N+  E  S ++E    + ++   K +++ N+  +LE +L          +++   + +C    +  G F++ I     + Q A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++  +D  L+WPF++KV +    L  QN    +I    P+     + RP+   N   G P F P++ L   + ++ D  + ++ +
Sbjct:  305 NREVERSSLTLEAFSHQHRLDQEKIENLSNKIRQLERSLTVKDLQLSEAEQMVRELQYC----TYDGIFVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLM---LLDQNNREHIIDAFRPDITSTSFQRPVSEMNIASGCPLFCPLAKLAGKSPYLRDDTIFIKAI 510
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 77.0258 bits (188), Expect = 7.805e-15
Identity = 51/213 (23.94%), Postives = 102/213 (47.89%), Query Frame = 1 
Query:  607 NQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTL----------KRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQN----LIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFV 1200
            N+  E  S ++E    + ++   K +++ N+  +LE +L          +++   + +C    +  G F++ I     + Q A+ G    + S  FY S  GY+M +++YL GDG      LS++ ++++  +D  L+WPF++KV +    L  QN    +I    P+     + RP+   N   G P F P++ L   + ++ D  + ++ +
Sbjct:  312 NREVERSSLTLEAFSHQHRLDQEKIENLSNKIRQLERSLTVKDLQLSEAEQMVRELQYC----TYDGIFVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLM---LLDQNNREHIIDAFRPDITSTSFQRPVSEMNIASGCPLFCPLAKLAGKSPYLRDDTIFIKAI 517
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000044583.1 (SMESG000044583.1)

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 1 
Query:  271 MPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLIVTILPNENPACYGRPIGCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQN 1209
            MPRQTECGCRFCSSCLNRHLEA DNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYI HKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNL VTILPNENPACYGRPIGCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQN
Sbjct:    1 MPRQTECGCRFCSSCLNRHLEATDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYIFHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLNVTILPNENPACYGRPIGCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQN 313
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000018435.1 (SMESG000018435.1)

HSP 1 Score: 107.071 bits (266), Expect = 6.213e-25
Identity = 57/144 (39.58%), Postives = 87/144 (60.42%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS--SCCLFSQNLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRF 1197
            G  I+ +  V+++I +A  G   +I+S PFY+   GYR  +KVY  GDG      LS+Y I+IKG++D  LRWPF +KV +S  S     QN+ + + P+ N  CYGRP+   N G G+P F+ +  L+ ++ FI  G +++RF
Sbjct:  330 GICIWTVRNVRQRITDAKSGFDTQITSKPFYLRQPGYRFSLKVYPNGDGQGKDRFLSIYFIIIKGEYDDELRWPFIEKVNLSLISSNALVQNISILMKPDSNMICYGRPVKEFNLGAGQPEFVNLGKLIDSDDFIHSGSIRIRF 473

HSP 2 Score: 84.7297 bits (208), Expect = 1.491e-17
Identity = 51/132 (38.64%), Postives = 74/132 (56.06%), Query Frame = 1 
Query:  175 FESFKENHNTFVFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLS--KFHLEKPEHLKSPENRPAKFYE 564
              SF +    F+     E  ++ CS C  IL  P QT+CGCRFC SC+N+H  + +N CPNC+  EI  E II DRAI++E++   I+C  + C  + +F+ L  HL   K+ LE  +  K P+  P +  E
Sbjct:   11 LASFDKRTEYFIKNIRLECPRFVCSKCHEILIDPSQTQCGCRFCYSCINKHFASCENICPNCQT-EIPNE-IIKDRAIDREISNLEIRCLLNGCDHIGQFKYLDDHLRMCKWRLENCD--KCPQTYPKQMKE 138
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000018409.1 (SMESG000018409.1)

HSP 1 Score: 106.686 bits (265), Expect = 6.679e-25
Identity = 57/144 (39.58%), Postives = 87/144 (60.42%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS--SCCLFSQNLIVTILPNENPACYGRPIG-CNQGFGEPSFIPISSLMLNNKFIIDGQLQLRF 1197
            G  I+ +  V+++I +A  G   +I+S PFY+   GYR  +KVY  GDG      LS+Y I+IKG++D  LRWPF +KV +S  S     QN+ + + P+ N  CYGRP+   N G G+P F+ +  L+ ++ FI  G +++RF
Sbjct:  328 GICIWTVRNVRQRITDAKSGFDTQITSKPFYLRQPGYRFSLKVYPNGDGQGKDRFLSIYFIIIKGEYDDELRWPFIEKVNLSLISSNALVQNISILMKPDSNMICYGRPVKEFNLGAGQPEFVNLGKLIDSDDFIHSGSIRIRF 471

HSP 2 Score: 79.337 bits (194), Expect = 1.079e-15
Identity = 46/113 (40.71%), Postives = 65/113 (57.52%), Query Frame = 1 
Query:  181 SFKENHNTFVFGSSSEIQQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLS--KFHLE 513
            S +     F+     E  ++ CS C  IL  P QT+CGCRFC SC+N+H  +  N CPNC+  EI  E II DRAI++E++   I+C  + C  + +F+ L  HL   K+ LE
Sbjct:   11 SLETRTEYFIKNIRLECPRFVCSKCHEILIDPSQTQCGCRFCYSCINKHFASCGNICPNCQT-EIPNE-IIKDRAIDREISNLEIRCLLNGCDHIGQFKYLDDHLKMCKWRLE 121
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000018404.1 (SMESG000018404.1)

HSP 1 Score: 100.138 bits (248), Expect = 8.796e-23
Identity = 53/147 (36.05%), Postives = 84/147 (57.14%), Query Frame = 1 
Query:  775 GQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVIS--SCCLFSQNLIVTILPNENPACYGRPI-GCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQ 1206
            G+ ++ I  +++KI  A  G   +I S PFY    GYRM +K+Y  GDG      LSVY ++++G++D  + WPF +KV +S  S     QN+ + + P+ N ACYG+PI   N G G+P FI +S L+ +  ++    ++L    Q
Sbjct:  298 GELVWIIENIRQKIHYAKSGNQVQIISEPFYSIRPGYRMRLKIYPNGDGTGKDKYLSVYFMIVQGEYDDEIAWPFRQKVSLSILSSKSARQNITLNMKPDNNVACYGKPINNSNVGAGQPEFIKLSELLTSLDYLHGTSIKLAIKVQ 444

HSP 2 Score: 65.4698 bits (158), Expect = 2.802e-11
Identity = 31/79 (39.24%), Postives = 48/79 (60.76%), Query Frame = 1 
Query:  256 KNILKMPRQTECGCRFCSSCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQAVCEFRQLIHH 492
            +N+   P QTECGCRFC +C+  ++   ++ CPNC + EI +   I D+A+EKE+    IKCP   C  +   ++L +H
Sbjct:    5 QNVFIEPIQTECGCRFCYNCIENYINNSNSSCPNC-DLEINRNKFIRDKAVEKEIFNHQIKCPNERCDEIFLLKELKNH 82
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000047296.1 (SMESG000047296.1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.261e-22
Identity = 90/340 (26.47%), Postives = 144/340 (42.35%), Query Frame = 1 
Query:  232 QQYFCSICKNILKMPRQTECGCRFCSSCLNRHLEAIDNFCP----NCKNFEIVKENIIV-DRAIEKELNKQIIKCPKSECQAVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTNQIR---EPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWISESGPGQ---------FIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYLIGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLIVTILPNENPACYGRPIGCNQ-GFGEPSFIPISSLMLNNKFIIDGQLQLRF 1197
            +++ C  CK+IL  P Q  CGCR C  C N+  E+    CP    NC   E    ++++ D++ E+++   +IKCP   C        L +HL++ +                  + I +     +  ST Q++   E K    E LKQK        DD       LE T++ I  +I    I  S   +         F++ I +  E   +A  G    I S PFY+S  GY+M IK+Y  G G      LS+Y ++++G +D  L WPF +K+ +      ++    T     N     RP        G P FI I  +  ++  +I+  L ++ 
Sbjct:   11 ERFKCINCKDILCSPVQLLCGCRMCLYCYNKVKESDVRRCPGNDINC--LEAFTSSVVMRDKSCERDMKMHLIKCPNVNCNENISIYNLKNHLNECN------------------QKIEADNNSVVLLSTKQVQDIFEIKEKIEELLKQK-------HDD---SKLNLELTIEEIRNSIQNLKIQYSNISKSTFILDNPVFLWPINRFSEAYDDAKSGKNTSIISKPFYVSEYGYKMSIKIYPNGHGEGLGTHLSIYFVILQGKYDPLLNWPFQEKISLELVGQKNKTFSETFKGPPNSEWTQRPKQERSISVGNPKFIEIGKITKSDDLLINDSLYIKM 320
The following BLAST results are available for this feature:
BLAST of TNF receptor-associated factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRAF28.081e-1833.33TNF receptor associated factor 2 [Source:HGNC Symb... [more]
TRAF51.245e-1633.78TNF receptor associated factor 5 [Source:HGNC Symb... [more]
TRAF51.245e-1633.78TNF receptor associated factor 5 [Source:HGNC Symb... [more]
TRAF51.245e-1633.78TNF receptor associated factor 5 [Source:HGNC Symb... [more]
TRAF17.730e-1526.81TNF receptor associated factor 1 [Source:HGNC Symb... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
trf-14.203e-1435.97TNF Receptor Associated Factor (TRAF) homolog; TRF... [more]
trf-21.908e-1032.39TNF Receptor Associated Factor (TRAF) homolog [So... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Traf42.864e-927.78gene:FBgn0026319 transcript:FBtr0334401[more]
Traf43.658e-927.78gene:FBgn0026319 transcript:FBtr0077429[more]
Traf69.444e-621.64gene:FBgn0265464 transcript:FBtr0071188[more]
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BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX901915.12.904e-1828.43pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:2... [more]
AL844899.12.904e-1828.43pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28... [more]
traf2a5.255e-1827.75Tnf receptor-associated factor 2a [Source:ZFIN;Acc... [more]
traf2b1.886e-1724.50Tnf receptor-associated factor 2b [Source:ZFIN;Acc... [more]
traf2b1.886e-1724.50Tnf receptor-associated factor 2b [Source:ZFIN;Acc... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRAF21.150e-1827.43TNF receptor associated factor 2 [Source:NCBI gene... [more]
TRAF28.519e-1832.02TNF receptor associated factor 2 [Source:NCBI gene... [more]
ssh24.793e-1329.73slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
ssh25.121e-1329.73slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
slc12a19.488e-1232.67solute carrier family 12 member 1 [Source:Xenbase;... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Traf27.053e-1727.36TNF receptor-associated factor 2 [Source:MGI Symbo... [more]
Traf27.254e-1727.36TNF receptor-associated factor 2 [Source:MGI Symbo... [more]
Traf51.982e-1631.54TNF receptor-associated factor 5 [Source:MGI Symbo... [more]
Traf51.982e-1631.54TNF receptor-associated factor 5 [Source:MGI Symbo... [more]
Traf56.028e-1631.54TNF receptor-associated factor 5 [Source:MGI Symbo... [more]
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BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q12933|TRAF2_HUMAN3.881e-1733.33TNF receptor-associated factor 2 OS=Homo sapiens O... [more]
sp|P39429|TRAF2_MOUSE4.934e-1627.36TNF receptor-associated factor 2 OS=Mus musculus O... [more]
sp|O00463|TRAF5_HUMAN5.977e-1633.78TNF receptor-associated factor 5 OS=Homo sapiens O... [more]
sp|P70191|TRAF5_MOUSE4.075e-1531.54TNF receptor-associated factor 5 OS=Mus musculus O... [more]
sp|P39428|TRAF1_MOUSE2.222e-1329.38TNF receptor-associated factor 1 OS=Mus musculus O... [more]
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BLAST of TNF receptor-associated factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2I0LIG54.599e-2628.41TNF receptor-associated factor 2 OS=Columba livia ... [more]
A0A384B3Z28.908e-2526.30TNF receptor-associated factor OS=Balaenoptera acu... [more]
T1D1222.531e-2229.65TRAF-5 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
T1DF653.326e-2231.90TRAF-2 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
A0A226NFY89.242e-2026.88TNF receptor-associated factor OS=Callipepla squam... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf2b8.084e-1828.21TNF receptor associated factor 2 [Source:NCBI gene... [more]
traf2a1.104e-1727.75TNF receptor-associated factor 2-like [Source:NCBI... [more]
traf2a1.213e-1727.75TNF receptor-associated factor 2-like [Source:NCBI... [more]
traf13.314e-1431.54TNF receptor-associated factor 1-like [Source:NCBI... [more]
traf13.410e-1431.54TNF receptor-associated factor 1-like [Source:NCBI... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000002130.13.055e-1529.73pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 g... [more]
ENSPMAT00000000074.12.830e-1330.95pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO406651.074e-1726.30Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494642.825e-1331.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO352371.356e-1127.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO328491.450e-1127.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412436.335e-1125.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf35.631e-2024.81TNF receptor associated factor 3 [Source:NCBI gene... [more]
ENSORLT00000020861.22.288e-1734.53TNF receptor-associated factor 2 [Source:NCBI gene... [more]
unm_sa8088.679e-1731.97TNF receptor-associated factor 2 [Source:NCBI gene... [more]
traf2b6.315e-1523.94TNF receptor associated factor 2 [Source:NCBI gene... [more]
traf2b7.805e-1523.94TNF receptor associated factor 2 [Source:NCBI gene... [more]
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BLAST of TNF receptor-associated factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000044583.10.000e+099.04SMESG000044583.1[more]
SMESG000018435.16.213e-2539.58SMESG000018435.1[more]
SMESG000018409.16.679e-2539.58SMESG000018409.1[more]
SMESG000018404.18.796e-2336.05SMESG000018404.1[more]
SMESG000047296.11.261e-2226.47SMESG000047296.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026063 ID=SMED30026063|Name=TNF receptor-associated factor|organism=Schmidtea mediterranea sexual|type=transcript|length=1262bp
AAGTTTTAATTGTTAAATATGACAACCAATCATTCATTTTGATGAATTTT
TTTCGCAATTTCATTCGTTGTTATTTTTAGAAATAACGTAATACGATTTA
CCAAATTTAAAAATGTATATAATCGTTTACCAAAACATTCTATTCATAAT
TTTTTCATTATGTATATCAATTATTTCGAAAGCTTTAAAGAAAATCATAA
TACATTTGTTTTTGGATCTTCAAGTGAAATCCAGCAATACTTTTGTTCGA
TTTGTAAAAATATATTAAAAATGCCAAGACAAACGGAATGTGGCTGTAGA
TTTTGTTCTTCATGCTTGAATAGGCATTTGGAAGCGATTGATAATTTTTG
TCCGAATTGTAAAAATTTTGAAATTGTCAAAGAAAATATTATTGTCGACA
GGGCCATTGAAAAAGAACTGAACAAACAAATCATAAAATGTCCAAAAAGT
GAATGTCAAGCCGTTTGTGAATTTAGGCAACTTATTCATCACCTTTCGAA
ATTCCATTTAGAAAAACCCGAGCATTTAAAATCACCAGAAAATCGTCCAG
CTAAATTCTATGAAAATATTCCTTCTACATTTTGTCAACATTTAGCACAT
TCGACCAATCAAATTCGAGAACCAAAATCAAATTCTATCGAGAAATTAAA
ACAAAAATCTCAGATTTCATTTGGAAAATTTGATGATGTTGGAAATCAAC
CAACCAGACTAGAGAGCACATTAAAAAGAATTGAGGAAAATATCGATTTT
TGTTGGATTTCCGAATCAGGTCCCGGACAATTTATCTTTCACATACCAAA
AGTACAGGAGAAAATCCAAAACGCTCTTGATGGAGGATTAAATGAGATTT
CAAGTGTGCCTTTTTACATTTCCCACAAAGGATACAGAATGGTAATTAAA
GTCTACTTAATCGGAGATGGAAACGTTCACAAAATCTCTCTATCGGTTTA
TTGTATTCTAATAAAAGGAGATTTTGACCATTTTCTTCGTTGGCCATTCA
ATAAGAAAGTTGTTATATCAAGCTGTTGTTTGTTTAGCCAGAATTTGATT
GTGACAATTTTGCCCAATGAAAATCCAGCTTGCTACGGCAGACCAATCGG
ATGTAATCAAGGTTTCGGGGAACCGAGTTTCATTCCAATCTCAAGTTTGA
TGTTGAACAATAAATTTATTATTGATGGGCAACTCCAACTCAGATTTGTC
TTTCAGAATTGAAACATTTGTCATAATCGTTTTTTTATATATAAAAATAG
AATTTAAATTAA
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protein sequence of SMED30026063-orf-1

>SMED30026063-orf-1 ID=SMED30026063-orf-1|Name=SMED30026063-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=351bp
MYINYFESFKENHNTFVFGSSSEIQQYFCSICKNILKMPRQTECGCRFCS
SCLNRHLEAIDNFCPNCKNFEIVKENIIVDRAIEKELNKQIIKCPKSECQ
AVCEFRQLIHHLSKFHLEKPEHLKSPENRPAKFYENIPSTFCQHLAHSTN
QIREPKSNSIEKLKQKSQISFGKFDDVGNQPTRLESTLKRIEENIDFCWI
SESGPGQFIFHIPKVQEKIQNALDGGLNEISSVPFYISHKGYRMVIKVYL
IGDGNVHKISLSVYCILIKGDFDHFLRWPFNKKVVISSCCLFSQNLIVTI
LPNENPACYGRPIGCNQGFGEPSFIPISSLMLNNKFIIDGQLQLRFVFQN
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0001817regulation of cytokine production
GO:0008063Toll signaling pathway
GO:0032088negative regulation of NF-kappaB transcription factor activity
GO:0033209tumor necrosis factor-mediated signaling pathway
GO:0042981regulation of apoptotic process
GO:0045087innate immune response
GO:0050688regulation of defense response to virus
GO:0007165signal transduction
GO:0007250activation of NF-kappaB-inducing kinase activity
GO:0016567protein ubiquitination
GO:0046330positive regulation of JNK cascade
Vocabulary: molecular function
TermDefinition
GO:0005164tumor necrosis factor receptor binding
GO:0008270zinc ion binding
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005622intracellular
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000028Category 2 cell
PLANA:0000034epidermis
PLANA:0000204ovary
PLANA:0000208testis
PLANA:0000224cement gland
Vocabulary: INTERPRO
TermDefinition
IPR018957Znf_C3HC4_RING-type
IPR043211TNF_rcpt-assoc_TRAF
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
IPR008974TRAF-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 29..67
e-value: 9.0E-5
score: 31.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 29..68
score: 10.734
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 7..122
e-value: 1.8E-16
score: 62.3
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 151..349
e-value: 1.3E-29
score: 105.2
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 205..345
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 29..67
e-value: 2.0E-4
score: 21.1
NoneNo IPR availablePANTHERPTHR10131:SF129TNF RECEPTOR-ASSOCIATED FACTOR 6coord: 25..114
NoneNo IPR availablePANTHERPTHR10131:SF129TNF RECEPTOR-ASSOCIATED FACTOR 6coord: 144..345
NoneNo IPR availableCDDcd16449RING-HCcoord: 29..67
e-value: 2.84592E-9
score: 50.1585
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 23..72
IPR043211TNF receptor-associated factor TRAFPANTHERPTHR10131TNF RECEPTOR ASSOCIATED FACTORcoord: 25..114
IPR043211TNF receptor-associated factor TRAFPANTHERPTHR10131TNF RECEPTOR ASSOCIATED FACTORcoord: 144..345