60S ribosomal protein L35a
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026049 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 143.28 bits (360), Expect = 1.718e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 143.28 bits (360), Expect = 1.718e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 117.087 bits (292), Expect = 2.210e-34 Identity = 53/92 (57.61%), Postives = 71/92 (77.17%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLP 374 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLP 93
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 79.337 bits (194), Expect = 5.057e-20 Identity = 35/53 (66.04%), Postives = 46/53 (86.79%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKK 257 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAKK Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKK 55
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 77.7962 bits (190), Expect = 1.789e-19 Identity = 34/52 (65.38%), Postives = 45/52 (86.54%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAK 254 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAK 54
BLAST of 60S ribosomal protein L35a vs. Ensembl Celegans
Match: rpl-33 (60S ribosomal protein L35a [Source:UniProtKB/Swiss-Prot;Acc:P49180]) HSP 1 Score: 137.887 bits (346), Expect = 1.391e-42 Identity = 60/124 (48.39%), Postives = 88/124 (70.97%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPI-GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 M+ V+ + + P GRLYVKA+FTG++RGLR Q E+T+LL+++GV ++D FY GK+ ++YKA +K G T+ R +WGK+ + HG +GAVRA+F N+P A+GKRIR++LYPS I Sbjct: 1 MADTAVARRPSAPTTGRLYVKAIFTGFKRGLRTQSEHTSLLKLEGVFNKEDAGFYAGKRVVYLYKAHNKTLKTGHTVATRTRAIWGKITRPHGNAGAVRAKFHHNIPPSALGKRIRVLLYPSNI 124
BLAST of 60S ribosomal protein L35a vs. Ensembl Celegans
Match: rpl-33 (60S ribosomal protein L35a [Source:UniProtKB/Swiss-Prot;Acc:P49180]) HSP 1 Score: 137.887 bits (346), Expect = 1.391e-42 Identity = 60/124 (48.39%), Postives = 88/124 (70.97%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPI-GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 M+ V+ + + P GRLYVKA+FTG++RGLR Q E+T+LL+++GV ++D FY GK+ ++YKA +K G T+ R +WGK+ + HG +GAVRA+F N+P A+GKRIR++LYPS I Sbjct: 1 MADTAVARRPSAPTTGRLYVKAIFTGFKRGLRTQSEHTSLLKLEGVFNKEDAGFYAGKRVVYLYKAHNKTLKTGHTVATRTRAIWGKITRPHGNAGAVRAKFHHNIPPSALGKRIRVLLYPSNI 124
BLAST of 60S ribosomal protein L35a vs. Ensembl Celegans
Match: rpl-33 (60S ribosomal protein L35a [Source:UniProtKB/Swiss-Prot;Acc:P49180]) HSP 1 Score: 137.887 bits (346), Expect = 1.391e-42 Identity = 60/124 (48.39%), Postives = 88/124 (70.97%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPI-GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 M+ V+ + + P GRLYVKA+FTG++RGLR Q E+T+LL+++GV ++D FY GK+ ++YKA +K G T+ R +WGK+ + HG +GAVRA+F N+P A+GKRIR++LYPS I Sbjct: 1 MADTAVARRPSAPTTGRLYVKAIFTGFKRGLRTQSEHTSLLKLEGVFNKEDAGFYAGKRVVYLYKAHNKTLKTGHTVATRTRAIWGKITRPHGNAGAVRAKFHHNIPPSALGKRIRVLLYPSNI 124
BLAST of 60S ribosomal protein L35a vs. Ensembl Fly
Match: RpL35A (gene:FBgn0037328 transcript:FBtr0078769) HSP 1 Score: 150.599 bits (379), Expect = 4.027e-47 Identity = 73/123 (59.35%), Postives = 91/123 (73.98%), Query Frame = 3 Query: 60 SSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAK-KAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 S KVS K GRL+ KAVFTGY+RGLRNQHEN A+L+I+G ++ FY+GK+C +VYKA+ K + + KT+VR VWGKV + HG +GAVRA+F +NLPG AMG RIRIMLYPSRI Sbjct: 35 SEAKVSAKKYKRHGRLFAKAVFTGYKRGLRNQHENQAILKIEGARRKEHGSFYVGKRCVYVYKAETKKCVPQHPERKTRVRAVWGKVTRIHGNTGAVRARFNRNLPGHAMGHRIRIMLYPSRI 157
BLAST of 60S ribosomal protein L35a vs. Ensembl Zebrafish
Match: rpl35a (ribosomal protein L35a [Source:ZFIN;Acc:ZDB-GENE-040718-190]) HSP 1 Score: 139.813 bits (351), Expect = 2.521e-43 Identity = 63/109 (57.80%), Postives = 85/109 (77.98%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+++GV + + +FY+GK+C +VYKAKK G + K R++WGKV +AHG SG VRA+FK NLP +A+G RIR+MLYPSR+ Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKLEGVYNRGEVDFYLGKRCAYVYKAKKKTATPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFKSNLPAKAIGHRIRVMLYPSRV 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Zebrafish
Match: rpl35a (ribosomal protein L35a [Source:ZFIN;Acc:ZDB-GENE-040718-190]) HSP 1 Score: 139.813 bits (351), Expect = 2.521e-43 Identity = 63/109 (57.80%), Postives = 85/109 (77.98%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+++GV + + +FY+GK+C +VYKAKK G + K R++WGKV +AHG SG VRA+FK NLP +A+G RIR+MLYPSR+ Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKLEGVYNRGEVDFYLGKRCAYVYKAKKKTATPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFKSNLPAKAIGHRIRVMLYPSRV 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Zebrafish
Match: rpl35a (ribosomal protein L35a [Source:ZFIN;Acc:ZDB-GENE-040718-190]) HSP 1 Score: 139.813 bits (351), Expect = 2.521e-43 Identity = 63/109 (57.80%), Postives = 85/109 (77.98%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+++GV + + +FY+GK+C +VYKAKK G + K R++WGKV +AHG SG VRA+FK NLP +A+G RIR+MLYPSR+ Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKLEGVYNRGEVDFYLGKRCAYVYKAKKKTATPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFKSNLPAKAIGHRIRVMLYPSRV 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Xenopus
Match: rpl35a (ribosomal protein L35a [Source:NCBI gene;Acc:448717]) HSP 1 Score: 141.354 bits (355), Expect = 8.300e-44 Identity = 64/109 (58.72%), Postives = 85/109 (77.98%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + + R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKATFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNRTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Match: Rpl35a (ribosomal protein L35A [Source:MGI Symbol;Acc:MGI:1928894]) HSP 1 Score: 142.895 bits (359), Expect = 1.656e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Match: Rpl35a (ribosomal protein L35A [Source:MGI Symbol;Acc:MGI:1928894]) HSP 1 Score: 142.895 bits (359), Expect = 1.656e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Match: Rpl35a (ribosomal protein L35A [Source:MGI Symbol;Acc:MGI:1928894]) HSP 1 Score: 142.895 bits (359), Expect = 1.656e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Match: Rpl35a (ribosomal protein L35A [Source:MGI Symbol;Acc:MGI:1928894]) HSP 1 Score: 142.895 bits (359), Expect = 1.656e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Match: Rpl35a (ribosomal protein L35A [Source:MGI Symbol;Acc:MGI:1928894]) HSP 1 Score: 142.895 bits (359), Expect = 1.656e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Match: sp|Q5R8K6|RL35A_PONAB (60S ribosomal protein L35a OS=Pongo abelii OX=9601 GN=RPL35A PE=3 SV=1) HSP 1 Score: 143.28 bits (360), Expect = 8.252e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Match: sp|P61272|RL35A_MACFA (60S ribosomal protein L35a OS=Macaca fascicularis OX=9541 GN=RPL35A PE=3 SV=1) HSP 1 Score: 143.28 bits (360), Expect = 8.252e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Match: sp|P18077|RL35A_HUMAN (60S ribosomal protein L35a OS=Homo sapiens OX=9606 GN=RPL35A PE=1 SV=2) HSP 1 Score: 143.28 bits (360), Expect = 8.252e-44 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Match: sp|P04646|RL35A_RAT (60S ribosomal protein L35a OS=Rattus norvegicus OX=10116 GN=Rpl35a PE=3 SV=1) HSP 1 Score: 142.895 bits (359), Expect = 1.158e-43 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Match: sp|O55142|RL35A_MOUSE (60S ribosomal protein L35a OS=Mus musculus OX=10090 GN=Rpl35a PE=1 SV=2) HSP 1 Score: 142.895 bits (359), Expect = 1.158e-43 Identity = 65/109 (59.63%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+I+GV + +TEFY+GK+C +VYKAK + G + K R++WGKV +AHG SG VRA+F+ NLP +A+G RIR+MLYPSRI Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI 110
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Match: A0A1S3K6P9 (60S ribosomal protein L35a OS=Lingula unguis OX=7574 GN=LOC106179120 PE=3 SV=1) HSP 1 Score: 172.94 bits (437), Expect = 5.856e-53 Identity = 74/123 (60.16%), Postives = 99/123 (80.49%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 M+ ++ GRLYVKAVFTGY+RGLRNQHENT+LL+++GVT +K TEFY+GK+CC+VYKAK + G D+ +++R++WGKV + HG SGAVRA+F+KNLP AMGKR+R+MLYPSR+ Sbjct: 1 MADQEAKQNPPKKSGRLYVKAVFTGYKRGLRNQHENTSLLKLEGVTTKKATEFYLGKRCCYVYKAKNKSACPGHDKASRIRVIWGKVTRPHGNSGAVRAKFRKNLPASAMGKRVRVMLYPSRV 123
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Match: A0A0R3SEQ5 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS3263 PE=3 SV=1) HSP 1 Score: 172.94 bits (437), Expect = 6.569e-53 Identity = 80/128 (62.50%), Postives = 100/128 (78.12%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPI-----GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 MS EK S + + GRLYVKAVFTGYRRGLRNQHENTALL+IDGV K+TEFY+GK+C ++Y+A+K T++R++WGK+IK HGRSGAVRAQFK+NLP +AMG+RIR+MLYPSR+ Sbjct: 1 MSEEKASAPTVKRVRQKQPGRLYVKAVFTGYRRGLRNQHENTALLKIDGVHTAKETEFYMGKRCAYIYRAEKKKHLPRRHNPTRIRVIWGKIIKPHGRSGAVRAQFKRNLPAKAMGQRIRVMLYPSRV 128
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Match: A0A0R3U4Z7 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS1754 PE=3 SV=1) HSP 1 Score: 171.014 bits (432), Expect = 4.714e-52 Identity = 74/109 (67.89%), Postives = 95/109 (87.16%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRLYVKA+FTGYRRGLRNQHENTALL+IDGV ++TEFY+GK+C ++Y+A+K + TK+R++WGK++K HGRSGAVRA+FK+NLPG+AMGKRIR+MLYPSR+ Sbjct: 20 GRLYVKAIFTGYRRGLRNQHENTALLKIDGVNTARETEFYMGKRCAYIYRAEKKKQLPRRHKPTKIRVIWGKIVKPHGRSGAVRARFKRNLPGKAMGKRIRVMLYPSRV 128
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Match: A0A3P7T8N8 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS9100 PE=3 SV=1) HSP 1 Score: 170.629 bits (431), Expect = 1.207e-51 Identity = 76/109 (69.72%), Postives = 94/109 (86.24%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRLYVKAVFTGYRRGLRNQHENTALL+IDGV K+TEFY+GK+C ++Y+A+K + T++R+VWGK+IK HGRSGAVRAQFK+NLP +AMG+RIR+MLYPSR+ Sbjct: 40 GRLYVKAVFTGYRRGLRNQHENTALLKIDGVHTAKETEFYMGKRCAYIYRAEKKKQLPRRHKPTRIRVVWGKIIKPHGRSGAVRAQFKRNLPAKAMGQRIRVMLYPSRV 148
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Match: A0A0R3TNW1 (Uncharacterized protein OS=Rodentolepis nana OX=102285 PE=3 SV=1) HSP 1 Score: 170.629 bits (431), Expect = 1.220e-51 Identity = 76/109 (69.72%), Postives = 94/109 (86.24%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRLYVKAVFTGYRRGLRNQHENTALL+IDGV K+TEFY+GK+C ++Y+A+K + T++R+VWGK+IK HGRSGAVRAQFK+NLP +AMG+RIR+MLYPSR+ Sbjct: 40 GRLYVKAVFTGYRRGLRNQHENTALLKIDGVHTAKETEFYMGKRCAYIYRAEKKKQLPRRHKPTRIRVVWGKIIKPHGRSGAVRAQFKRNLPAKAMGQRIRVMLYPSRV 148
BLAST of 60S ribosomal protein L35a vs. Ensembl Cavefish
Match: rpl35a (60S ribosomal protein L35a [Source:NCBI gene;Acc:111189478]) HSP 1 Score: 139.428 bits (350), Expect = 3.133e-43 Identity = 61/109 (55.96%), Postives = 86/109 (78.90%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 GRL+ KA+F GY+RGLRNQ E+TALL+++GV +++ +FY+GK+C +VYKAKK + G + K R++WGKV +AHG SG VRA+F NLP +A+G RIR+M+YPSR+ Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKVEGVYTRQEVDFYLGKRCAYVYKAKKNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFSSNLPAKAIGHRIRVMMYPSRV 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Cavefish
Match: rpl35a (60S ribosomal protein L35a [Source:NCBI gene;Acc:111189478]) HSP 1 Score: 136.732 bits (343), Expect = 3.788e-42 Identity = 60/109 (55.05%), Postives = 85/109 (77.98%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 G L+ KA+F GY+RGLRNQ E+TALL+++GV +++ +FY+GK+C +VYKAKK + G + K R++WGKV +AHG SG VRA+F NLP +A+G RIR+M+YPSR+ Sbjct: 3 GWLWCKAIFAGYKRGLRNQREHTALLKVEGVYTRQEVDFYLGKRCAYVYKAKKNTVTPG-GKPNKTRVIWGKVTRAHGNSGMVRAKFSSNLPAKAIGHRIRVMMYPSRV 110
BLAST of 60S ribosomal protein L35a vs. Ensembl Cavefish
Match: RPL35A (ribosomal protein L35a [Source:HGNC Symbol;Acc:HGNC:10345]) HSP 1 Score: 97.4413 bits (241), Expect = 8.399e-27 Identity = 43/81 (53.09%), Postives = 62/81 (76.54%), Query Frame = 3 Query: 99 GRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSG 341 GRL+ KA+F GY+RGLRNQ E+TALL+++GV +++ +FY+GK+C +VYKAKK + G + K R++WGKV +AHG G Sbjct: 3 GRLWCKAIFAGYKRGLRNQREHTALLKVEGVYTRQEVDFYLGKRCAYVYKAKKNTVTPG-GKPNKTRVIWGKVTRAHGNKG 82
BLAST of 60S ribosomal protein L35a vs. Ensembl Sea Lamprey
Match: rpl35a (ribosomal protein L35a [Source:ZFIN;Acc:ZDB-GENE-040718-190]) HSP 1 Score: 150.214 bits (378), Expect = 4.366e-48 Identity = 68/111 (61.26%), Postives = 91/111 (81.98%), Query Frame = 3 Query: 93 PIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 P RL+ KA+FTGY+RGLRNQHE+TALL+I+GV + +T FY+GK+C +VYKA+K + G + K R++WGKV+++HG SG VRA+F+KNLPG+AMG RIR+MLYPSRI Sbjct: 9 PGCRLWCKAIFTGYKRGLRNQHEHTALLKIEGVYTRGETGFYLGKRCAYVYKARKNTLTPG-GKPNKTRVIWGKVMRSHGNSGVVRAKFRKNLPGKAMGHRIRVMLYPSRI 118
BLAST of 60S ribosomal protein L35a vs. Ensembl Yeast
Match: RPL33A (Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication [Source:SGD;Acc:S000006064]) HSP 1 Score: 102.834 bits (255), Expect = 8.591e-30 Identity = 47/108 (43.52%), Postives = 67/108 (62.04%), Query Frame = 3 Query: 102 RLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 RLYVK Y+R R + N +L++I+GV +D +FY+GK+ +VY+A K +K+R++WGKV + HG SG VRA F+ NLP + G +RI LYPS I Sbjct: 6 RLYVKGKHLSYQRSKRVNNPNVSLIKIEGVATPQDAQFYLGKRIAYVYRASKEV------RGSKIRVMWGKVTRTHGNSGVVRATFRNNLPAKTFGASVRIFLYPSNI 107
BLAST of 60S ribosomal protein L35a vs. Ensembl Yeast
Match: RPL33B (Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication [Source:SGD;Acc:S000005760]) HSP 1 Score: 100.908 bits (250), Expect = 5.117e-29 Identity = 46/108 (42.59%), Postives = 67/108 (62.04%), Query Frame = 3 Query: 102 RLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 RLYVK Y+R R + N +L++I+GV ++ +FY+GK+ +VY+A K +K+R++WGKV + HG SG VRA F+ NLP + G +RI LYPS I Sbjct: 6 RLYVKGKHLSYQRSKRVNNPNVSLIKIEGVATPQEAQFYLGKRIAYVYRASKEV------RGSKIRVMWGKVTRTHGNSGVVRATFRNNLPAKTFGASVRIFLYPSNI 107
BLAST of 60S ribosomal protein L35a vs. Ensembl Nematostella
Match: EDO46062 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR70]) HSP 1 Score: 142.895 bits (359), Expect = 5.036e-45 Identity = 61/108 (56.48%), Postives = 85/108 (78.70%), Query Frame = 3 Query: 102 RLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 RLY K + G++RGLRNQH NT+L++I+GV E+K+TEFY+GK+ FVY+AK + G + TK+R++WGKV +AHG SG VRA+F+ NLP +AMG +R+MLYPSR+ Sbjct: 6 RLYTKGIVLGFKRGLRNQHPNTSLVKIEGVDERKNTEFYLGKRLAFVYRAKNKTVAKGDKKATKLRVIWGKVTRAHGNSGVVRAKFRHNLPPKAMGATVRVMLYPSRV 113
BLAST of 60S ribosomal protein L35a vs. Ensembl Medaka
Match: rpl35a (ribosomal protein L35a [Source:ZFIN;Acc:ZDB-GENE-040718-190]) HSP 1 Score: 67.3958 bits (163), Expect = 1.604e-15 Identity = 28/49 (57.14%), Postives = 40/49 (81.63%), Query Frame = 3 Query: 102 RLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYK 248 RL+ KA+FTGY+RGLRNQ E+TALL+++G + +FY+GK+C +VYK Sbjct: 4 RLWSKAIFTGYKRGLRNQREHTALLKLEGCYSRDQVDFYLGKRCAYVYK 52
BLAST of 60S ribosomal protein L35a vs. Planmine SMEST
Match: SMESG000003977.1 (SMESG000003977.1) HSP 1 Score: 253.062 bits (645), Expect = 3.529e-88 Identity = 122/123 (99.19%), Postives = 123/123 (100.00%), Query Frame = 3 Query: 57 MSSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFDEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 425 MSSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGF+EKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI Sbjct: 1 MSSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDTEFYIGKKCCFVYKAKKAAMKAGFEEKTKVRIVWGKVIKAHGRSGAVRAQFKKNLPGQAMGKRIRIMLYPSRI 123 The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L35a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of 60S ribosomal protein L35a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of 60S ribosomal protein L35a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of 60S ribosomal protein L35a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 3
BLAST of 60S ribosomal protein L35a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of 60S ribosomal protein L35a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of 60S ribosomal protein L35a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 60S ribosomal protein L35a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 60S ribosomal protein L35a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of 60S ribosomal protein L35a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of 60S ribosomal protein L35a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of 60S ribosomal protein L35a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of 60S ribosomal protein L35a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of 60S ribosomal protein L35a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026049 ID=SMED30026049|Name=60S ribosomal protein L35a|organism=Schmidtea mediterranea sexual|type=transcript|length=455bpback to top protein sequence of SMED30026049-orf-1 >SMED30026049-orf-1 ID=SMED30026049-orf-1|Name=SMED30026049-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp MSSEKVSNKSTTPIGRLYVKAVFTGYRRGLRNQHENTALLRIDGVTEQKDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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