RING finger protein 32

Overview


Name	RING finger protein 32
Smed ID	SMED30025982
Length (bp)	1119

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of RING finger protein 32 (SMED30025982) t-SNE clustered cells

Violin plots show distribution of expression levels for RING finger protein 32 (SMED30025982) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RING finger protein 32 (SMED30025982) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RING finger protein 32 (SMED30025982) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30025982

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30025982	SMESG000057989.1	SmedASXL_010780	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30025982	SMESG000057989.1	dd_Smed_v4_12150_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30025982	SMESG000057989.1	dd_Smed_v4_12150_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30025982	SMESG000057989.1	dd_Smed_v6_12150_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
epidermis	SMED30025982	SMESG000057989.1	dd_Smed_v4_12150_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of RING finger protein 32 vs. Ensembl Human
Match: RNF32 (ring finger protein 32 [Source:HGNC Symbol;Acc:HGNC:17118])

HSP 1 Score: 207.994 bits (528), Expect = 2.242e-63
Identity = 125/362 (34.53%), Postives = 196/362 (54.14%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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BLAST of RING finger protein 32 vs. Ensembl Human
Match: RNF32 (ring finger protein 32 [Source:HGNC Symbol;Acc:HGNC:17118])

HSP 1 Score: 162.155 bits (409), Expect = 2.018e-46
Identity = 103/302 (34.11%), Postives = 165/302 (54.64%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQ 906
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I        Q E S C +
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKI------QVQNETSACFE 292

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BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Match: rnf32 (ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-061013-39])

HSP 1 Score: 177.948 bits (450), Expect = 5.153e-52
Identity = 108/338 (31.95%), Postives = 176/338 (52.07%), Query Frame = 1

Query:   58 SIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQ-------SWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDR 1050
            ++ A A Q+HIT +++V   +   L     +C        A    ++         E+ +Y+L D    + +LA+ +GL+E P   +T + W + K  +I  G    PC IC+++F+LQP +LLSCSHVFH+ C++SFE+FS KK CP+CR   Y+  + +     Y  +CA +I+  W GY  R  Y   R+  PPK   L +++ E K  E+ D  +   + T+V+ F+ DID ++  S      + + +  R     + W    +K+++   ++C ICL        S  +NRN ++LSCSH+ H++CL  FE       E +  CPLCR  Y +
Sbjct:   15 TLAAVALQEHITHKISV-GYHPHKLHVSDASCPAAGGAAHASRRNKSQNSRQAQGKEEREYVL-DPAPPRLTLAQRMGLVEAPSPRLTAEEWAEVKTRSILEGDSTQPCAICREEFRLQPQVLLSCSHVFHKVCLKSFEKFSGKKCCPMCRKEQYETRVIYDGARFYREKCALRIQTLWRGYVVRKWYSNIRKHVPPKDQHLRRRFFEKKFQEMNDSLVQSCS-TDVEEFLNDIDRSVATSHDILRRFNN-KYMRIRMEDEDWLQAQKKAVQRGVQDCPICLNHLTSHNGSSKENRNILLLSCSHLFHEVCLQAFELF---CQEVKPTCPLCRSHYHK 345

HSP 2 Score: 51.6026 bits (122), Expect = 8.026e-7
Identity = 30/87 (34.48%), Postives = 50/87 (57.47%), Query Frame = 1

Query:  307 KIVITEQIWKKQKQFAISRGYFALPCPICQDDF--------KLQPTLLLSCSHVFHRNCIESFERFSN--KKSCPICRTINYQKMIT 537
            +I + ++ W + ++ A+ RG     CPIC +          + +  LLLSCSH+FH  C+++FE F    K +CP+CR+ +Y KM+ 
Sbjct:  265 RIRMEDEDWLQAQKKAVQRG--VQDCPICLNHLTSHNGSSKENRNILLLSCSHLFHEVCLQAFELFCQEVKPTCPLCRS-HYHKMLV 348

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BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Match: rnf32 (ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-061013-39])

HSP 1 Score: 177.178 bits (448), Expect = 9.421e-52
Identity = 107/338 (31.66%), Postives = 178/338 (52.66%), Query Frame = 1

Query:   58 SIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQ-------SWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDR 1050
            ++ A A Q+HIT +++V   +   L     +C        A    ++         E+ +Y+L D    + +LA+ +GL+E P   +T + W + K  +I  G    PC IC+++F+LQP +LLSCSHVFH+ C++SFE+FS KK CP+CR   Y+  + +    +Y  +CA +I+  W GY  R  Y   R+  PPK   L +++ E K  E+ +  +   + T+V+ F+ DID ++  S      + + + +R     + W    +K+++   ++C ICL        S  +NRN ++LSCSH+ H++CL  FE       E +  CPLCR  Y +
Sbjct:   15 TLAAIALQEHITHKISV-GYHPHKLHVSDASCPAAGGAAHASRRNKSQNSRQAQGKEEREYVL-DPAPPRLTLAQRMGLVEAPSPRLTAEEWAEVKTRSILEGDSTQPCAICREEFRLQPQVLLSCSHVFHKVCLKSFEKFSGKKCCPMCRKEQYETRVIYDGARLYREKCALRIQTLWRGYVVRKWYSNIRKHVPPKDQHLRRRFFEKKFQEMNESLVQSCS-TDVEEFLNDIDRSVATSHDILRHFNN-KYTRIRMEDEDWLQAQKKAVQRGVQDCPICLNHLTSHKGSSKENRNILLLSCSHLFHEVCLQAFELF---CQEVKPTCPLCRSHYHK 345

HSP 2 Score: 51.9878 bits (123), Expect = 6.306e-7
Identity = 30/87 (34.48%), Postives = 48/87 (55.17%), Query Frame = 1

Query:  307 KIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPT--------LLLSCSHVFHRNCIESFERFSN--KKSCPICRTINYQKMIT 537
            +I + ++ W + ++ A+ RG     CPIC +               LLLSCSH+FH  C+++FE F    K +CP+CR+ +Y KM+ 
Sbjct:  265 RIRMEDEDWLQAQKKAVQRG--VQDCPICLNHLTSHKGSSKENRNILLLSCSHLFHEVCLQAFELFCQEVKPTCPLCRS-HYHKMLV 348

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BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Match: CU137686.1 (pep chromosome:GRCz11:CHR_ALT_CTG22_1_28:36930127:36938313:1 gene:ENSDARG00000093859.4 transcript:ENSDART00000074105.6 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU137686.1)

HSP 1 Score: 51.9878 bits (123), Expect = 6.288e-7
Identity = 23/51 (45.10%), Postives = 32/51 (62.75%), Query Frame = 1

Query:  358 SRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICR 510
            S   + L CP+CQD FK    ++LSCSH F + C++ F R  N + CP+CR
Sbjct:    3 SSAEYDLTCPVCQDIFKT--PVILSCSHSFCKECLQQFWRNKNTQECPVCR 51

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BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Match: trim35-20 (tripartite motif containing 35-20 [Source:ZFIN;Acc:ZDB-GENE-110920-8])

HSP 1 Score: 49.6766 bits (117), Expect = 3.390e-6
Identity = 21/51 (41.18%), Postives = 31/51 (60.78%), Query Frame = 1

Query:  358 SRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICR 510
            S   +   CP+CQD FK    ++LSCSH F + C++ F +  N + CP+CR
Sbjct:    3 SSAEYDYSCPVCQDIFKT--PVILSCSHSFCKECLQQFWKIKNTQECPVCR 51

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BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Match: trim35-33 (tripartite motif containing 35-33 [Source:ZFIN;Acc:ZDB-GENE-081031-1])

HSP 1 Score: 49.2914 bits (116), Expect = 5.085e-6
Identity = 21/43 (48.84%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  382 CPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICR 510
            CP+CQD FK    ++LSCSH F + C++ F R  N + CP+CR
Sbjct:   11 CPVCQDIFKT--PVILSCSHSFCQECLQQFWRSKNTQECPVCR 51

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BLAST of RING finger protein 32 vs. Ensembl Xenopus
Match: gpr150 (G protein-coupled receptor 150 [Source:Xenbase;Acc:XB-GENE-940801])

HSP 1 Score: 199.904 bits (507), Expect = 3.689e-60
Identity = 111/300 (37.00%), Postives = 174/300 (58.00%), Query Frame = 1

Query:  187 VTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVM-DRWELILQKSMKWSQKECSICLQS--WSKNRNR--------VVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            V +  +KP I    +E+Y+L D      +LA+ LG++  P + +T + W K KQ ++ +G    PC IC+++F + P +LLSCSHVFHR C+E+FERF+ +K+CP+CR   YQ  + +    +Y  +CA++I+A W GY  R  Y++ R+T PPK+  L +K+ E K  EVTDR L   + TN++ F  +ID +L  S+    +    EGSR +++  + WE I  K+ +    +C IC+    +S +  R        V+LSCSH+ H+ CL  FE     +    +ICPLCR  Y ++
Sbjct:   91 VIKNTKKPAI----EEEYVL-DPAPPPLTLAQKLGIVGAPALPLTHEEWIKVKQRSVDQGDSLQPCVICKEEFGIHPQVLLSCSHVFHRACLEAFERFTGRKTCPMCRRNQYQTRVIYDGARLYKAKCATRIQACWRGYIVRKWYKQLRQTIPPKNPKLRRKFFEEKFTEVTDRLLRSFS-TNIEVFFNEIDHSLANSRKVLRQ---VEGSRASQISEEEWERIQIKAARQEISDCPICIMPLCYSSDTQRNGQLLRPVVLLSCSHVFHQACLHAFEEFTVGE---PYICPLCRSTYQKK 378

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BLAST of RING finger protein 32 vs. Ensembl Mouse
Match: Rnf32 (ring finger protein 32 [Source:MGI Symbol;Acc:MGI:1861747])

HSP 1 Score: 183.341 bits (464), Expect = 4.511e-54
Identity = 123/357 (34.45%), Postives = 191/357 (53.50%), Query Frame = 1

Query:   52 NASIVAAAFQDHITQRLNV--LSVNDE---SLSXXXXXXXXXXXXXXAVVVT---RAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS---------------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDH+   L +  LSV D       K +K  K  K    A++ T   ++ + P +   EKE Y+L D      +LA+ LGL+  P + ++   W++ KQ ++ +G    PCPIC+++F+L P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +CA++I+AYW GY  R  Y+  R+  PP  + L +K+ E K  E++ R L   N TN+D   ++ID  L  ++   ++  +  G    K  + WE I  ++      ECSICL   S               + R  V+LSC+H+ H  CLL  E     D+   H+CPLCR  Y ++
Sbjct:   13 NLAVTAVALQDHLLHDLQLGHLSVADPCKIKARKKEKKSKSLKRDATAIIDTGLRKSTEGPNMEDPEKE-YVL-DPTPPPLTLAQKLGLLPPPPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPSDAKLRRKFFEEKFTEISHRILCSYN-TNIDELFSEIDVCLAVNRSILQQLDERCGQIITK--EDWEKIQAQAAHHEIYECSICLTPLSFHGDGRQAAIGTSSQRPRETVLLSCAHLFHNACLLALEEFSLGDNAPFHVCPLCRSCYQKK 364

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Query:   52 NASIVAAAFQDHITQRLNV--LSVNDE---SLSXXXXXXXXXXXXXXAVVVT---RAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS---------------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDH+   L +  LSV D       K +K  K  K    A++ T   ++ + P +   EKE Y+L D      +LA+ LGL+  P + ++   W++ KQ ++ +G    PCPIC+++F+L P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +CA++I+AYW GY  R  Y+  R+  PP  + L +K+ E K  E++ R L   N TN+D   ++ID  L  ++   ++  +  G    K  + WE I  ++      ECSICL   S               + R  V+LSC+H+ H  CLL  E     D+   H+CPLCR  Y ++
Sbjct:   13 NLAVTAVALQDHLLHDLQLGHLSVADPCKIKARKKEKKSKSLKRDATAIIDTGLRKSTEGPNMEDPEKE-YVL-DPTPPPLTLAQKLGLLPPPPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPSDAKLRRKFFEEKFTEISHRILCSYN-TNIDELFSEIDVCLAVNRSILQQLDERCGQIITK--EDWEKIQAQAAHHEIYECSICLTPLSFHGDGRQAAIGTSSQRPRETVLLSCAHLFHNACLLALEEFSLGDNAPFHVCPLCRSCYQKK 364

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Query:   52 NASIVAAAFQDHITQRLNV--LSVNDE---SLSXXXXXXXXXXXXXXAVVVT---RAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS---------------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDH+   L +  LSV D       K +K  K  K    A++ T   ++ + P +   EKE Y+L D      +LA+ LGL+  P + ++   W++ KQ ++ +G    PCPIC+++F+L P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +CA++I+AYW GY  R  Y+  R+  PP  + L +K+ E K  E++ R L   N TN+D   ++ID  L  ++   ++  +  G    K  + WE I  ++      ECSICL   S               + R  V+LSC+H+ H  CLL  E     D+   H+CPLCR  Y ++
Sbjct:   13 NLAVTAVALQDHLLHDLQLGHLSVADPCKIKARKKEKKSKSLKRDATAIIDTGLRKSTEGPNMEDPEKE-YVL-DPTPPPLTLAQKLGLLPPPPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPSDAKLRRKFFEEKFTEISHRILCSYN-TNIDELFSEIDVCLAVNRSILQQLDERCGQIITK--EDWEKIQAQAAHHEIYECSICLTPLSFHGDGRQAAIGTSSQRPRETVLLSCAHLFHNACLLALEEFSLGDNAPFHVCPLCRSCYQKK 364

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BLAST of RING finger protein 32 vs. Ensembl Mouse
Match: Rnf32 (ring finger protein 32 [Source:MGI Symbol;Acc:MGI:1861747])

HSP 1 Score: 138.658 bits (348), Expect = 5.261e-38
Identity = 92/291 (31.62%), Postives = 144/291 (49.48%), Query Frame = 1

Query:  193 RAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKV--HEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDI 1059
            ++ + P +   EKE Y+L D      +LA+ LGL+  P + ++   W++ KQ ++ +G    PCPIC+++F+L P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +CA++I+AYW GY  R  Y+  R+  PP  + L +K+ E K   HE+   Y   I  T +                       F G      +                       S  + R  V+LSC+H+ H  CLL  E     D+   H+CPLCR  Y ++ +
Sbjct:   31 KSTEGPNMEDPEKE-YVL-DPTPPPLTLAQKLGLLPPPPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPSDAKLRRKFFEEKAAHHEI---YECSICLTPL----------------------SFHGDGRQAAIG---------------------TSSQRPRETVLLSCAHLFHNACLLALEEFSLGDNAPFHVCPLCRSCYQKKIV 273

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BLAST of RING finger protein 32 vs. UniProt/SwissProt
Match: sp|Q4R5T4|RNF32_MACFA (RING finger protein 32 OS=Macaca fascicularis OX=9541 GN=RNF32 PE=2 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 1.836e-66
Identity = 127/362 (35.08%), Postives = 199/362 (54.97%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI + L +  LSV D S ++++K            ++   ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILRDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHGGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEITE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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BLAST of RING finger protein 32 vs. UniProt/SwissProt
Match: sp|Q9H0A6|RNF32_HUMAN (RING finger protein 32 OS=Homo sapiens OX=9606 GN=RNF32 PE=1 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 1.077e-62
Identity = 125/362 (34.53%), Postives = 196/362 (54.14%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICY----AEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            ++   ++K T C     +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFH+ C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW G   R  Y+  R+T PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   ++ ++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDQCLAINRSVLQQLEEKCGHEITE--EEWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

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BLAST of RING finger protein 32 vs. UniProt/SwissProt
Match: sp|Q9JIT1|RNF32_MOUSE (RING finger protein 32 OS=Mus musculus OX=10090 GN=Rnf32 PE=1 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 3.156e-53
Identity = 123/357 (34.45%), Postives = 191/357 (53.50%), Query Frame = 1

Query:   52 NASIVAAAFQDHITQRLNV--LSVNDE---SLSXXXXXXXXXXXXXXAVVVT---RAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS---------------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDH+   L +  LSV D       K +K  K  K    A++ T   ++ + P +   EKE Y+L D      +LA+ LGL+  P + ++   W++ KQ ++ +G    PCPIC+++F+L P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +CA++I+AYW GY  R  Y+  R+  PP  + L +K+ E K  E++ R L   N TN+D   ++ID  L  ++   ++  +  G    K  + WE I  ++      ECSICL   S               + R  V+LSC+H+ H  CLL  E     D+   H+CPLCR  Y ++
Sbjct:   13 NLAVTAVALQDHLLHDLQLGHLSVADPCKIKARKKEKKSKSLKRDATAIIDTGLRKSTEGPNMEDPEKE-YVL-DPTPPPLTLAQKLGLLPPPPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPSDAKLRRKFFEEKFTEISHRILCSYN-TNIDELFSEIDVCLAVNRSILQQLDERCGQIITK--EDWEKIQAQAAHHEIYECSICLTPLSFHGDGRQAAIGTSSQRPRETVLLSCAHLFHNACLLALEEFSLGDNAPFHVCPLCRSCYQKK 364

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BLAST of RING finger protein 32 vs. TrEMBL
Match: A0A2K6ECJ2 (Ring finger protein 32 OS=Macaca nemestrina OX=9545 GN=RNF32 PE=4 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 6.462e-64
Identity = 127/362 (35.08%), Postives = 199/362 (54.97%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI + L +  LSV D S ++++K            ++   ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILRDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEMTE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

[Close]

BLAST of RING finger protein 32 vs. TrEMBL
Match: F7HSS1 (Ring finger protein 32 OS=Macaca mulatta OX=9544 GN=RNF32 PE=4 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 6.462e-64
Identity = 127/362 (35.08%), Postives = 199/362 (54.97%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI + L +  LSV D S ++++K            ++   ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILRDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEMTE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

[Close]

BLAST of RING finger protein 32 vs. TrEMBL
Match: A0A2K5W6P7 (Uncharacterized protein OS=Macaca fascicularis OX=9541 PE=4 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 6.462e-64
Identity = 127/362 (35.08%), Postives = 199/362 (54.97%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI + L +  LSV D S ++++K            ++   ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILRDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEMTE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 358

[Close]

BLAST of RING finger protein 32 vs. TrEMBL
Match: A0A2K5Z6H7 (Ring finger protein 32 OS=Mandrillus leucophaeus OX=9568 GN=RNF32 PE=4 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 1.153e-63
Identity = 128/362 (35.36%), Postives = 197/362 (54.42%), Query Frame = 1

Query:   31 MNKRGHY----NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            +  +GH     N ++ A A QDHI   L +  LSV D S ++++K            V    ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    2 LKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAVTDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEITE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPLHACPLCRSCYQKK 358

[Close]

BLAST of RING finger protein 32 vs. TrEMBL
Match: G7P2J6 (Uncharacterized protein (Fragment) OS=Macaca fascicularis OX=9541 GN=EGM_13144 PE=4 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.398e-63
Identity = 125/351 (35.61%), Postives = 195/351 (55.56%), Query Frame = 1

Query:   52 NASIVAAAFQDHITQRLNV--LSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQK----PTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWS-----------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDHI + L +  LSV D S ++++K            ++   ++K    P +  +EKE Y+L D K    +LA+ LGLI  P   ++   W+K KQ ++ +G    PCPIC+++F+L+P +LLSCSHVFHR C+++FE+F+NKK+CP+CR   YQ  +      ++  +C ++I+AYW GY  R  Y+  RET PP  + L KK+ E K  E++ R L   N TN++   A+ID  L  ++   +++++  G    +  + WE I  ++++    ECSICL   S           ++R   +LSCSH+ H  CLL  E     D    H CPLCR  Y ++
Sbjct:    8 NLAVNAVALQDHILRDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTMQCPKLEDSEKE-YVL-DPKPPPLTLAQKLGLIGPPPPQLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHRACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGYVVRKWYRNLRETVPPTDAKLRKKFFEKKFTEISHRILCSYN-TNIEELFAEIDRCLAINRSVLQQFEEKYGHEMTE--EEWEKIQVQALRRETHECSICLAPLSPAGGQRVGAGQRSRETALLSCSHVFHHACLLALEEFSVGDRPPFHACPLCRSCYQKK 353

[Close]

BLAST of RING finger protein 32 vs. Ensembl Cavefish
Match: rnf32 (ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-061013-39])

HSP 1 Score: 143.665 bits (361), Expect = 3.098e-39
Identity = 95/327 (29.05%), Postives = 170/327 (51.99%), Query Frame = 1

Query:   13 LLSYSSMNKRGHYNASIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGS--RTNKVMDRWELILQKSMKWSQKECSICLQSW-SKNRNRVVLSCSHILHKICLLTFE 984
            +L   S ++      ++ AAA QDHIT+ L+     +   +   ++ + ++      ++            E+++Y+L + +    +LA+ LGL+  P   +T + W + K  ++  G  A PC IC ++F+LQP +LLSCSHVFH  C++S ERFS ++ CP+CR   Y+  +      +Y  +CA +I+  W G+  R +Y+  R+  PP    L +++ E K  E+ +  +     ++V+ F+ D+D T+  S+  F  +   + S        + W    +K+++    +C ICL    S +R  ++LSCSH+LH+ CL  FE
Sbjct:    1 MLKGRSSSREDRRKLAVAAAALQDHITRSLSGSEGPNIFPAAPSRSHRARQHRHTGFIL-----------GEEQEYIL-EPEAPPLTLAQRLGLVAAPAGRLTAEEWAEVKSRSLQEGDSAQPCVICTEEFRLQPQVLLSCSHVFHMVCLKSLERFSGRRCCPLCRREPYETRVIHDGAKLYREKCAVRIQTCWRGFAARKSYRAVRKLIPPNHPQLRRRYFEEKFQEINESLVRSCC-SDVEVFLLDVDQTVAASRDVFRLFDQQQKSVLEEEGEEEEWRKAREKAVQRDISDCPICLTPLHSTSRQVLLLSCSHLLHESCLQAFE 314

[Close]

BLAST of RING finger protein 32 vs. Ensembl Cavefish
Match: rnft1 (ring finger protein, transmembrane 1 [Source:NCBI gene;Acc:103046121])

HSP 1 Score: 50.0618 bits (118), Expect = 2.110e-6
Identity = 26/50 (52.00%), Postives = 34/50 (68.00%), Query Frame = 1

Query:  382 CPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKM 531
            CPICQ DFK QP +LL C H+F   CI  +  F+ +K+CP+CRTI   K+
Sbjct:  364 CPICQSDFK-QPRVLL-CQHIFCEECITQW--FNLEKTCPLCRTIITDKV 409

[Close]

BLAST of RING finger protein 32 vs. Ensembl Nematostella
Match: EDO33138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST21])

HSP 1 Score: 158.303 bits (399), Expect = 6.808e-45
Identity = 113/375 (30.13%), Postives = 174/375 (46.40%), Query Frame = 1

Query:   52 NASIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENK-------------VHEVTDRYLNW------------INETNVDNFIADIDDTLEKSKCAFEEWQDFEGS--RTNKVMDRWELILQKSMKWSQKECSICLQSWS--------------KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            N ++ A A QDH  + L   S+ D    KI  +              +  + P    + KE Y+L D      +LA+  GL+E P+ ++++  W+  KQ + +R    LPC IC++DF     +LLSCSHVFHR C+E+FE+FS KK CP+CR   YQK I       +  +C  +I+A W GY  R  Y+K R+T  PK   L +K+ ENK               +     L W             +   VDN +++I+ +L  S+    +    EG   ++      WE    K+++    +C IC+   +              K+R  V+LSCSH+ H  CL  FE   +     +H+CP+CR  Y ++
Sbjct:   24 NTALTAVALQDHWARSL---SLKDPVKKKINHSLPVAAKPRSTAARIKGGKGPETRESNKE-YVL-DPVPPPPTLAQKYGLVEAPEKLLSDTDWQSVKQQSNARLDSTLPCVICKEDFGNLQQVLLSCSHVFHRTCLEAFEKFSGKKCCPMCRKEKYQKRIIHEGAKQHRVKCVIRIQAAWRGYVLRSWYRKLRQTVAPKDPKLRQKFYENKDFVNGPPLTPDSASSDDPKEPLQWGPFVLQTHLSILASTATVDNLMSEINQSLASSRNIMSQ---IEGDCIKSPISSQEWEQARLKAVERCTTDCPICIMPLAQQGLYNRETSACAGKHRPSVLLSCSHVFHMTCLQAFEEFTT---STKHVCPVCRATYQKR 387

HSP 2 Score: 54.299 bits (129), Expect = 3.437e-8
Identity = 40/110 (36.36%), Postives = 55/110 (50.00%), Query Frame = 1

Query:  271 SLAESLGLIEQP-----KIVITEQIWKKQKQFAISRGYFALPCPIC-----QDDF----------KLQPTLLLSCSHVFHRNCIESFERF--SNKKSCPICRTINYQKMI 534
            SLA S  ++ Q      K  I+ Q W++ +  A+ R      CPIC     Q             K +P++LLSCSHVFH  C+++FE F  S K  CP+CR   YQK +
Sbjct:  282 SLASSRNIMSQIEGDCIKSPISSQEWEQARLKAVER--CTTDCPICIMPLAQQGLYNRETSACAGKHRPSVLLSCSHVFHMTCLQAFEEFTTSTKHVCPVCRA-TYQKRV 388

[Close]

BLAST of RING finger protein 32 vs. Ensembl Nematostella
Match: EDO29619 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T346])

HSP 1 Score: 82.0333 bits (201), Expect = 9.529e-19
Identity = 56/165 (33.94%), Postives = 87/165 (52.73%), Query Frame = 1

Query:   58 SIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDF----KLQPTLLLSCSHVFHRNCIESFERF--SNKKSCPICRTINYQKMI 534
            ++ A A QDH  + L   S+ D    KI  +              +  + P    + KE Y+L D      +LA+  GL+E P+ ++++  W+  KQ + +R    LPC IC++DF    + +P++LLSCSHVFH  C+++FE F  S K  CP+CR   YQK +
Sbjct:    3 ALTAVALQDHWARSL---SLKDPVKKKINHSLPVAAKPRSTAARIKGGKGPETRESNKE-YVL-DPVPPPPTLAQKYGLVEAPEKLLSDTDWQSVKQQSNARLDSTLPCVICKEDFGNLQQHRPSVLLSCSHVFHMTCLQAFEEFTTSTKHVCPVCRA-TYQKRV 161

HSP 2 Score: 47.3654 bits (111), Expect = 1.262e-6
Identity = 25/75 (33.33%), Postives = 40/75 (53.33%), Query Frame = 1

Query:  844 WELILQKSMKW--SQKECSICLQSWS---KNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQ 1053
            W+ + Q+S     S   C IC + +    ++R  V+LSCSH+ H  CL  FE   +     +H+CP+CR  Y ++
Sbjct:   89 WQSVKQQSNARLDSTLPCVICKEDFGNLQQHRPSVLLSCSHVFHMTCLQAFEEFTT---STKHVCPVCRATYQKR 160

[Close]

BLAST of RING finger protein 32 vs. Ensembl Medaka
Match: rnf32 (ring finger protein 32 [Source:NCBI gene;Acc:101159303])

HSP 1 Score: 180.644 bits (457), Expect = 2.366e-53
Identity = 116/366 (31.69%), Postives = 195/366 (53.28%), Query Frame = 1

Query:   19 SYSSMNKRGHYNASIVAAAFQDHITQRL--NVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHE--VTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMD-RWELILQKSMKWSQKECSICLQS-WSKN-------------RNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDI 1059
            ++ + N +   N +I +AAFQDHI + L     SV+D  LS+  +  +      K   + R         A++E   +        +LA+ LGL+  PK  +T+  W + K+ ++ R   A PC IC+++F+LQP +LL CSHVFHR C+++FERFS +K CP+CR   Y+  +      ++ ++CA +I+AYW GY  R +Y++   T  PK   L +++ E++V +  + D ++   + T+  +F+ DID +L  S+  F   Q  E  R +K  +  W+ I  +  +   ++C ICL + W  +             R  ++LSCSH+ H+ CL  FEA  +   +++  CPLCR  Y ++ I
Sbjct:    1 TFKNKNHKTSNNWAITSAAFQDHIIRSLLSEDFSVSD-PLSRHSRKSRKPPSREKLEDLKRGD-------AQQETKHVFKPTPPNLTLAQKLGLVASPKERLTKGDWMEVKKRSLLREESAQPCTICREEFRLQPQVLLCCSHVFHRTCLQAFERFSGRKRCPVCRREQYETRVIHDAARLFRHQCAIRIQAYWRGYSARKSYREIMRTKCPKDKKLRRRFFEDRVSKEFLNDSFVRHCH-TDTKDFLRDIDRSLSSSRRVF---QQLEKKRPSKPQENNWDQIKTQVFQRGVRDCPICLTALWDTSFPRASGISAHLHPRPTLLLSCSHLFHQFCLEAFEAFIA---DRRPTCPLCRSPYHKKLI 351

[Close]

BLAST of RING finger protein 32 vs. Ensembl Medaka
Match: rnf32 (ring finger protein 32 [Source:NCBI gene;Acc:101159303])

HSP 1 Score: 159.073 bits (401), Expect = 3.690e-45
Identity = 96/293 (32.76%), Postives = 160/293 (54.61%), Query Frame = 1

Query:   40 RGHYNASIVAAAFQDHITQRL--NVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMD-RWELILQKSMKWSQKECSICLQS 909
            R   N +I +AAFQDHI + L     SV+D  LS+  +  +      K   + R         A++E   +        +LA+ LGL+  PK  +T+  W + K+ ++ R   A PC IC+++F+LQP +LL CSHVFHR C+++FERFS +K CP+CR   Y+  +      ++ ++CA +I+AYW GY  R +Y++   T  PK   L +++ E+++ E+ D ++   + T+  +F+ DID +L  S+  F   Q  E  R +K  +  W+ I  +  +   ++C ICL +
Sbjct:   10 RTSNNWAITSAAFQDHIIRSLLSEDFSVSD-PLSRHSRKSRKPPSREKLEDLKRG-------DAQQETKHVFKPTPPNLTLAQKLGLVASPKERLTKGDWMEVKKRSLLREESAQPCTICREEFRLQPQVLLCCSHVFHRTCLQAFERFSGRKRCPVCRREQYETRVIHDAARLFRHQCAIRIQAYWRGYSARKSYREIMRTKCPKDKKLRRRFFEDRLQELNDSFVRHCH-TDTKDFLRDIDRSLSSSRRVF---QQLEKKRPSKPQENNWDQIKTQVFQRGVRDCPICLTA 290

[Close]

BLAST of RING finger protein 32 vs. Planmine SMEST
Match: SMESG000057989.1 (SMESG000057989.1)

HSP 1 Score: 694.115 bits (1790), Expect = 0.000e+0
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 1

Query:   31 MNKRGHYNASIVAAAFQDHITQRLNVLSVNDESLSXXXXXXXXXXXXXXAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDIHALDYTYI 1083
            MNKRGHYNASIVAAAFQDHITQRLNVLSVNDESLSKIKKNCKYKKPIGKAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDIHALDYTYI
Sbjct:    1 MNKRGHYNASIVAAAFQDHITQRLNVLSVNDESLSKIKKNCKYKKPIGKAVVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQIWKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERFSNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKYRETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKSKCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWSKNRNRVVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDIHALDYTYI 351

The following BLAST results are available for this feature:

BLAST of RING finger protein 32 vs. Ensembl Human
BLAST of RING finger protein 32 vs. Ensembl Celegans
BLAST of RING finger protein 32 vs. Ensembl Fly
BLAST of RING finger protein 32 vs. Ensembl Zebrafish
BLAST of RING finger protein 32 vs. Ensembl Xenopus
BLAST of RING finger protein 32 vs. Ensembl Mouse
BLAST of RING finger protein 32 vs. UniProt/SwissProt
BLAST of RING finger protein 32 vs. TrEMBL
BLAST of RING finger protein 32 vs. Ensembl Cavefish
BLAST of RING finger protein 32 vs. Ensembl Sea Lamprey
BLAST of RING finger protein 32 vs. Ensembl Yeast
BLAST of RING finger protein 32 vs. Ensembl Nematostella
BLAST of RING finger protein 32 vs. Ensembl Medaka
BLAST of RING finger protein 32 vs. Planmine SMEST

BLAST of RING finger protein 32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RNF32	2.242e-63	34.53	ring finger protein 32 [Source:HGNC Symbol;Acc:HGN...	[more]
RNF32	2.242e-63	34.53	ring finger protein 32 [Source:HGNC Symbol;Acc:HGN...	[more]
RNF32	2.242e-63	34.53	ring finger protein 32 [Source:HGNC Symbol;Acc:HGN...	[more]
RNF32	2.242e-63	34.53	ring finger protein 32 [Source:HGNC Symbol;Acc:HGN...	[more]
RNF32	2.018e-46	34.11	ring finger protein 32 [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of RING finger protein 32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of RING finger protein 32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of RING finger protein 32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnf32	5.153e-52	31.95	ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
rnf32	9.421e-52	31.66	ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
CU137686.1	6.288e-7	45.10	pep chromosome:GRCz11:CHR_ALT_CTG22_1_28:36930127:...	[more]
trim35-20	3.390e-6	41.18	tripartite motif containing 35-20 [Source:ZFIN;Acc...	[more]
trim35-33	5.085e-6	48.84	tripartite motif containing 35-33 [Source:ZFIN;Acc...	[more]

BLAST of RING finger protein 32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gpr150	3.689e-60	37.00	G protein-coupled receptor 150 [Source:Xenbase;Acc...	[more]

BLAST of RING finger protein 32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Rnf32	4.511e-54	34.45	ring finger protein 32 [Source:MGI Symbol;Acc:MGI:...	[more]
Rnf32	4.511e-54	34.45	ring finger protein 32 [Source:MGI Symbol;Acc:MGI:...	[more]
Rnf32	4.511e-54	34.45	ring finger protein 32 [Source:MGI Symbol;Acc:MGI:...	[more]
Rnf32	5.261e-38	31.62	ring finger protein 32 [Source:MGI Symbol;Acc:MGI:...	[more]

BLAST of RING finger protein 32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3


Match Name	E-value	Identity	Description
sp\|Q4R5T4\|RNF32_MACFA	1.836e-66	35.08	RING finger protein 32 OS=Macaca fascicularis OX=9...	[more]
sp\|Q9H0A6\|RNF32_HUMAN	1.077e-62	34.53	RING finger protein 32 OS=Homo sapiens OX=9606 GN=...	[more]
sp\|Q9JIT1\|RNF32_MOUSE	3.156e-53	34.45	RING finger protein 32 OS=Mus musculus OX=10090 GN...	[more]

BLAST of RING finger protein 32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2K6ECJ2	6.462e-64	35.08	Ring finger protein 32 OS=Macaca nemestrina OX=954...	[more]
F7HSS1	6.462e-64	35.08	Ring finger protein 32 OS=Macaca mulatta OX=9544 G...	[more]
A0A2K5W6P7	6.462e-64	35.08	Uncharacterized protein OS=Macaca fascicularis OX=...	[more]
A0A2K5Z6H7	1.153e-63	35.36	Ring finger protein 32 OS=Mandrillus leucophaeus O...	[more]
G7P2J6	1.398e-63	35.61	Uncharacterized protein (Fragment) OS=Macaca fasci...	[more]

BLAST of RING finger protein 32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rnf32	3.098e-39	29.05	ring finger protein 32 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
rnft1	2.110e-6	52.00	ring finger protein, transmembrane 1 [Source:NCBI ...	[more]

BLAST of RING finger protein 32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of RING finger protein 32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of RING finger protein 32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO33138	6.808e-45	30.13	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29619	9.529e-19	33.94	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of RING finger protein 32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rnf32	2.366e-53	31.69	ring finger protein 32 [Source:NCBI gene;Acc:10115...	[more]
rnf32	3.690e-45	32.76	ring finger protein 32 [Source:NCBI gene;Acc:10115...	[more]

BLAST of RING finger protein 32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000057989.1	0.000e+0	100.00	SMESG000057989.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30025982 ID=SMED30025982|Name=RING finger protein 32|organism=Schmidtea mediterranea sexual|type=transcript|length=1119bp
CGATCTATCTAGTTACTAAGCTACAGCTCTATGAATAAAAGGGGGCATTA
CAACGCCAGCATTGTTGCTGCTGCGTTCCAGGATCATATAACTCAACGTC
TTAATGTTTTATCTGTGAATGACGAAAGTCTGTCCAAAATTAAAAAAAAT
TGCAAGTACAAAAAACCAATTGGAAAAGCAGTAGTTGTTACTAGAGCTAT
ACAAAAGCCTACAATTTGTTATGCTGAAAAAGAGGATTATTTATTAAACG
ACTCAAAGCATCATAAATCATCACTTGCCGAATCTCTTGGGCTAATAGAA
CAACCGAAAATAGTTATTACTGAACAGATTTGGAAAAAGCAGAAGCAGTT
TGCTATTAGTCGTGGATATTTTGCTTTACCTTGTCCAATTTGTCAAGACG
ACTTTAAATTGCAACCCACCTTGTTGCTATCGTGCAGCCACGTGTTTCAT
CGAAATTGCATAGAATCTTTTGAAAGATTTTCAAATAAAAAATCTTGTCC
GATCTGCAGAACAATAAATTACCAAAAAATGATAACATTTTTACCCGAGA
ATATATACATAAATAGATGCGCCTCAAAAATTCGAGCTTATTGGCTTGGC
TATAAAGAACGGCTAAATTACCAGAAGTACCGGGAAACTCATCCACCAAA
ATCAAGTATTTTAAACAAGAAATGGTTGGAAAACAAAGTTCACGAGGTCA
CTGATCGGTATTTGAATTGGATAAATGAAACAAATGTAGACAATTTCATT
GCGGATATTGATGATACTCTAGAGAAATCAAAATGTGCTTTTGAAGAATG
GCAGGATTTCGAGGGATCTCGGACTAATAAAGTCATGGATAGATGGGAGT
TAATTTTGCAAAAGTCTATGAAGTGGAGTCAAAAGGAATGTTCTATTTGC
TTGCAGAGCTGGTCTAAAAACCGAAATAGAGTAGTATTGTCATGCTCACA
CATTTTACACAAAATATGTTTGCTGACATTTGAAGCAATTCGATCAAGGG
ATCATGAAAAGCAACATATATGTCCGTTGTGTAGAGAAGTGTATGACCGG
CAGGACATTCATGCACTTGATTATACATATATTTAATCCTGCAAAACACT
ATTCACTGTGAGATCGGAA

protein sequence of SMED30025982-orf-1

>SMED30025982-orf-1 ID=SMED30025982-orf-1|Name=SMED30025982-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=352bp
MNKRGHYNASIVAAAFQDHITQRLNVLSVNDESLSKIKKNCKYKKPIGKA
VVVTRAIQKPTICYAEKEDYLLNDSKHHKSSLAESLGLIEQPKIVITEQI
WKKQKQFAISRGYFALPCPICQDDFKLQPTLLLSCSHVFHRNCIESFERF
SNKKSCPICRTINYQKMITFLPENIYINRCASKIRAYWLGYKERLNYQKY
RETHPPKSSILNKKWLENKVHEVTDRYLNWINETNVDNFIADIDDTLEKS
KCAFEEWQDFEGSRTNKVMDRWELILQKSMKWSQKECSICLQSWSKNRNR
VVLSCSHILHKICLLTFEAIRSRDHEKQHICPLCREVYDRQDIHALDYTY
I*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR042862	RNF32
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR000048	IQ_motif_EF-hand-BS

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0046872	metal ion binding

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 118..159 e-value: 6.8E-8 score: 42.2 coord: 287..334 e-value: 2.0E-5 score: 34.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 118..160 e-value: 5.8E-10 score: 39.4 coord: 285..335 e-value: 1.8E-7 score: 31.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 118..160 score: 13.419
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 287..335 score: 11.589
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 276..351 e-value: 1.8E-10 score: 42.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 102..205 e-value: 1.7E-13 score: 52.3
IPR042862	RING finger protein 32	PANTHER	PTHR14991	FAMILY NOT NAMED	coord: 6..341
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 177..206 score: 6.742
None	No IPR available	CDD	cd16677	RING1-H2_RNF32	coord: 117..160 e-value: 1.71415E-23 score: 89.3106
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 101..167
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 269..341

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RING finger protein 32

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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