SAP30-binding protein

Overview
NameSAP30-binding protein
Smed IDSMED30025974
Length (bp)1176
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SAP30-binding protein (SMED30025974) t-SNE clustered cells

Violin plots show distribution of expression levels for SAP30-binding protein (SMED30025974) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SAP30-binding protein (SMED30025974) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SAP30-binding protein (SMED30025974) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025974

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30025974SMESG000069710.1 SmedASXL_007053SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30025974SMESG000069710.1 SmedASXL_007053SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SAP30-binding protein vs. Ensembl Human
Match: SAP30BP (SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC:30785])

HSP 1 Score: 75.8702 bits (185), Expect = 1.313e-16
Identity = 43/89 (48.31%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:  645 EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:    5 EIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 93
[Close]
BLAST of SAP30-binding protein vs. Ensembl Human
Match: SAP30BP (SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC:30785])

HSP 1 Score: 80.1073 bits (196), Expect = 1.594e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  100 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 204
[Close]
BLAST of SAP30-binding protein vs. Ensembl Human
Match: SAP30BP (SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC:30785])

HSP 1 Score: 76.2554 bits (186), Expect = 1.751e-16
Identity = 43/89 (48.31%), Postives = 60/89 (67.42%), Query Frame = 3 
Query:  645 EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:    5 EIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 93
[Close]
BLAST of SAP30-binding protein vs. Ensembl Human
Match: SAP30BP (SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC:30785])

HSP 1 Score: 79.337 bits (194), Expect = 2.248e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:   84 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 188
[Close]
BLAST of SAP30-binding protein vs. Ensembl Human
Match: SAP30BP (SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC:30785])

HSP 1 Score: 80.4925 bits (197), Expect = 3.085e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  117 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 221
[Close]
BLAST of SAP30-binding protein vs. Ensembl Celegans
Match: Y67D2.7 (pep chromosome:WBcel235:III:1555395:1558529:-1 gene:WBGene00022057.1 transcript:Y67D2.7.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y67D2.7)

HSP 1 Score: 51.9878 bits (123), Expect = 2.588e-7
Identity = 24/67 (35.82%), Postives = 38/67 (56.72%), Query Frame = 3 
Query:  711 MRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
             R KA  +  N  Q I  N++Y NP  Y   I+  +IDE+GTN+   ++DP+ F +  +Y+ +A  Q
Sbjct:  222 FRQKALGTDLN--QQIQGNQQYNNPMSYTTFIDAFEIDEKGTNFAKNIFDPHVFPDNCFYDAIADEQ 286
[Close]
BLAST of SAP30-binding protein vs. Ensembl Celegans
Match: Y67D2.7 (pep chromosome:WBcel235:III:1555410:1558529:-1 gene:WBGene00022057.1 transcript:Y67D2.7.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y67D2.7)

HSP 1 Score: 51.9878 bits (123), Expect = 2.588e-7
Identity = 24/67 (35.82%), Postives = 38/67 (56.72%), Query Frame = 3 
Query:  711 MRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
             R KA  +  N  Q I  N++Y NP  Y   I+  +IDE+GTN+   ++DP+ F +  +Y+ +A  Q
Sbjct:  222 FRQKALGTDLN--QQIQGNQQYNNPMSYTTFIDAFEIDEKGTNFAKNIFDPHVFPDNCFYDAIADEQ 286
[Close]
BLAST of SAP30-binding protein vs. Ensembl Fly
Match: CG2063 (gene:FBgn0033400 transcript:FBtr0088600)

HSP 1 Score: 93.9745 bits (232), Expect = 3.421e-21
Identity = 52/116 (44.83%), Postives = 71/116 (61.21%), Query Frame = 3 
Query:  684 AELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWD 1031
            AEL  KIT+M  K   +  ++ ++I   KE+RNPSIY+KLI    I+E GTNYPPE+YDP ++GEESYYE LA  Q     KR ++           TA  + ++ E + RK+KWD
Sbjct:  171 AELVAKITKMYTKMNQTNMDMNRVIQDRKEFRNPSIYDKLISFCDINEFGTNYPPEIYDPLQWGEESYYESLAAVQKTEMVKR-QKDRKDMDKVEQATALARKVEEEAKKRKSKWD 285
[Close]
BLAST of SAP30-binding protein vs. Ensembl Zebrafish
Match: sap30bp (SAP30 binding protein [Source:ZFIN;Acc:ZDB-GENE-030131-457])

HSP 1 Score: 84.3445 bits (207), Expect = 6.983e-18
Identity = 62/161 (38.51%), Postives = 93/161 (57.76%), Query Frame = 3 
Query:  468 EGRESDIENDDTHDRPPRDVDLK-SRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQ 941
            E  E   EN+D+ +   R+ D + S SG+    VK +   +    +EL    S ++ N S  EI++PP P G+  + L++KI ++  +      +    I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ   E  +LE+
Sbjct:   51 EAYEKPSENEDSDEDSTRNSDEEESDSGRIQDDVKEVQEVEVKDPNELVAQFSEKVRNMSPDEIRIPPEPPGRCSSHLQEKIFKLYERKLQGDFDTNSHIQKKKEFRNPSIYEKLIQFCDIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ-KVEMDKLEK 210
[Close]
BLAST of SAP30-binding protein vs. Ensembl Xenopus
Match: SAP30BP (SAP30 binding protein [Source:NCBI gene;Acc:100379701])

HSP 1 Score: 86.2705 bits (212), Expect = 7.752e-19
Identity = 39/74 (52.70%), Postives = 55/74 (74.32%), Query Frame = 3 
Query:  690 LEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            L++KI ++  K  N R ++ ++I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:   62 LQEKIQKLYEKKLNERMDMNRIIQKKKEFRNPSIYEKLIQFCSIDELGTNYPKDMFDPHGWSEDSYYESLAKAQ 135
[Close]
BLAST of SAP30-binding protein vs. Ensembl Xenopus
Match: SAP30BP (SAP30 binding protein [Source:NCBI gene;Acc:100379701])

HSP 1 Score: 85.5001 bits (210), Expect = 2.706e-18
Identity = 39/74 (52.70%), Postives = 55/74 (74.32%), Query Frame = 3 
Query:  690 LEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            L++KI ++  K  N R ++ ++I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  106 LQEKIQKLYEKKLNERMDMNRIIQKKKEFRNPSIYEKLIQFCSIDELGTNYPKDMFDPHGWSEDSYYESLAKAQ 179
[Close]
BLAST of SAP30-binding protein vs. Ensembl Xenopus
Match: SAP30BP (SAP30 binding protein [Source:NCBI gene;Acc:100379701])

HSP 1 Score: 85.5001 bits (210), Expect = 3.487e-18
Identity = 39/74 (52.70%), Postives = 55/74 (74.32%), Query Frame = 3 
Query:  690 LEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            L++KI ++  K  N R ++ ++I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  124 LQEKIQKLYEKKLNERMDMNRIIQKKKEFRNPSIYEKLIQFCSIDELGTNYPKDMFDPHGWSEDSYYESLAKAQ 197
[Close]
BLAST of SAP30-binding protein vs. Ensembl Mouse
Match: Sap30bp (SAP30 binding protein [Source:MGI Symbol;Acc:MGI:1927479])

HSP 1 Score: 80.4925 bits (197), Expect = 1.974e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  100 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 204
[Close]
BLAST of SAP30-binding protein vs. UniProt/SwissProt
Match: sp|Q02614|S30BP_MOUSE (SAP30-binding protein OS=Mus musculus OX=10090 GN=Sap30bp PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 1.381e-15
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  100 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 204
[Close]
BLAST of SAP30-binding protein vs. UniProt/SwissProt
Match: sp|Q9UHR5|S30BP_HUMAN (SAP30-binding protein OS=Homo sapiens OX=9606 GN=SAP30BP PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.784e-15
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = 3 
Query:  603 ELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
            EL    S ++ N S  EIK+PP P G+    L+ KI ++  +      ++  +I   KE+RNPSIYEKLI+   IDE GTNYP +++DP+ + E+SYYE LAKAQ
Sbjct:  100 ELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQ 204
[Close]
BLAST of SAP30-binding protein vs. TrEMBL
Match: A0A069DR34 (Putative transcriptional regulator OS=Panstrongylus megistus OX=65343 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.022e-19
Identity = 79/238 (33.19%), Postives = 126/238 (52.94%), Query Frame = 3 
Query:  477 ESDIENDDTHDRP---PRDVDLKSRSGQSSSQVKAI----VMTKRPKGSELPYLLSRQIFNQSEIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDA---------AERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK*IISCF 1142
            E D  + DTH++P   P  +D KS +      + ++    V++ +    E P L   + +N  +++LPP P+G+   EL++KI  +  K K    ++ Q+I   K +RNPSIYEKLI+   I+E GTNY P++YDP ++ + SYY+ LAKAQ A          ER ++E L        + ++T    + EL+ RK+KWD   S  Q++ +  +G   +V  T     T   +IS F
Sbjct:   17 EDDESHRDTHEKPVLKPSGIDNKSVNKVGGKSIVSVTNENVISAQVISDEEPSL---EFYN--DVELPPEPIGRCSIELQEKIATLTEKMKIKSVDMNQIIQERKSFRNPSIYEKLIQFCDINEFGTNYDPQIYDPLKWDKSSYYDSLAKAQKADMDQREKERKERTKVEFLIGTAKKATNGSST----EEELKKRKSKWDQKSSTVQSTVTQPAG---IVTLTSSATGTKGTVISAF 242
[Close]
BLAST of SAP30-binding protein vs. TrEMBL
Match: A0A0V0G705 (Putative transcriptional regulator OS=Triatoma dimidiata OX=72491 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.032e-19
Identity = 79/238 (33.19%), Postives = 126/238 (52.94%), Query Frame = 3 
Query:  477 ESDIENDDTHDRP---PRDVDLKSRSGQSSSQVKAI----VMTKRPKGSELPYLLSRQIFNQSEIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDA---------AERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK*IISCF 1142
            E D  + DTH++P   P  +D KS +      + ++    V++ +    E P L   + +N  +++LPP P+G+   EL++KI  +  K K    ++ Q+I   K +RNPSIYEKLI+   I+E GTNY P++YDP ++ + SYY+ LAKAQ A          ER ++E L        + ++T    + EL+ RK+KWD   S  Q++ +  +G   +V  T     T   +IS F
Sbjct:   17 EDDESHRDTHEKPVSKPSGIDNKSVNKIGGKSIVSVTNENVISAQVISDEEPTL---EFYN--DVELPPEPIGRCSIELQEKIATLTEKMKIKSVDMNQIIQERKSFRNPSIYEKLIQFCDINEFGTNYDPQIYDPLKWDKSSYYDSLAKAQKADMDQREKERKERTKVEFLIGTAKKATNGSST----EEELKKRKSKWDQKSSTVQSTVTQPAG---IVTLTSSATGTKGTVISAF 242
[Close]
BLAST of SAP30-binding protein vs. TrEMBL
Match: A0A224XHY4 (Putative transcriptional regulator OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.129e-19
Identity = 79/238 (33.19%), Postives = 126/238 (52.94%), Query Frame = 3 
Query:  477 ESDIENDDTHDRP---PRDVDLKSRSGQSSSQVKAI----VMTKRPKGSELPYLLSRQIFNQSEIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDA---------AERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK*IISCF 1142
            E D  + DTH++P   P  +D K  +  S   + ++    V++ +    E P L   + +N  +++LPP P+G+   EL++KI  +  K K    ++ Q+I   K +RNPSIYEKLI+   I+E GTNY P++YDP ++ + SYY+ LAKAQ A          ER ++E L        + ++T    + EL+ RK+KWD   S  Q++ +  +G   +V  T     T   +IS F
Sbjct:   17 EDDESHRDTHEKPVSKPSGIDNKPLNKVSGKSIVSVTNENVISAQVISDEEPSL---EFYN--DVELPPEPVGRCSIELQEKIATLTEKMKIKSVDMNQIIQERKSFRNPSIYEKLIQFCDINEFGTNYDPQIYDPLKWDKSSYYDSLAKAQKADMDQREKERKERTKVEFLIGTAKKATNGSST----EEELKKRKSKWDQKSSTVQSTVTQPAG---IVTLTSSATGTKGTVISAF 242
[Close]
BLAST of SAP30-binding protein vs. TrEMBL
Match: A0A182ITX0 (Uncharacterized protein OS=Anopheles atroparvus OX=41427 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.319e-19
Identity = 51/115 (44.35%), Postives = 75/115 (65.22%), Query Frame = 3 
Query:  687 ELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWD 1031
            EL+ KI  +  + KNS  +  ++I   KE+RNPSIYEKLI+   I+E GTNYPPE++DP+++G+ESYYE LAKAQ   E +++E+          +T   ++   E + RK+KWD
Sbjct:  160 ELQDKIAALHERMKNSNMDTNRIIQERKEFRNPSIYEKLIQFCDINELGTNYPPEVFDPFQWGKESYYEELAKAQ-KIEMEKVEKARKEATKTEIQTGVKRMDPDESKKRKSKWD 273
[Close]
BLAST of SAP30-binding protein vs. TrEMBL
Match: U5EGX1 (Putative transcriptional regulator protein hcngp OS=Corethrella appendiculata OX=1370023 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.413e-19
Identity = 62/157 (39.49%), Postives = 90/157 (57.32%), Query Frame = 3 
Query:  684 AELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWD----TGQSKPQTSKSHSSGSTQVVIETKVMFATHK*IISCF 1142
             EL++KI+ +  K KNS  +  ++I   KE+RNPSIYEKLI+   I+E GTNYPPE+YDP +FG+ESYYE LA+ Q   E +++E+    +      +A  K  + + + RK+KWD    +  S+ QT K  S         T  +  T   +IS F
Sbjct:  166 VELQEKISNLYEKMKNSNMDTNRIIQDRKEFRNPSIYEKLIQFCDINELGTNYPPEIYDPLQFGKESYYEELARGQ-KIEMEKIEKARKESQKNDLTSAAKK-AEEDAKKRKSKWDQPAVSSSSQSQTIKPVSGIIQSQQTLTSNITGTKTTVISAF 320
[Close]
BLAST of SAP30-binding protein vs. Ensembl Cavefish
Match: sap30bp (SAP30 binding protein [Source:NCBI gene;Acc:103047331])

HSP 1 Score: 87.0409 bits (214), Expect = 7.724e-19
Identity = 63/168 (37.50%), Postives = 101/168 (60.12%), Query Frame = 3 
Query:  447 NDECFNV-EGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQ 941
            +DE   V +G E+  EN+D+ +      +  S SG+   ++K ++  +R   +EL    S ++ N S  EI++PP P G+ P  L++KI ++  +  +   +    I   KE+RNPSIYEKLI++  IDE GTNYP +++D + + E+SYYE LAKAQ   E ++LE+
Sbjct:   44 DDEVSRVKDGEENASENEDSDENDRNSEEDDSESGEPQDELKEVITGERRDPNELVAQFSEKVRNMSPEEIRIPPEPPGRCPGHLQEKIYKLYERKLHGDFDTNSHIQKKKEFRNPSIYEKLIQYCGIDELGTNYPKDMFDSHGWSEDSYYESLAKAQ-KVEMEKLEK 210
[Close]
BLAST of SAP30-binding protein vs. Ensembl Cavefish
Match: sap30bp (SAP30 binding protein [Source:NCBI gene;Acc:103047331])

HSP 1 Score: 81.2629 bits (199), Expect = 7.124e-17
Identity = 63/172 (36.63%), Postives = 101/172 (58.72%), Query Frame = 3 
Query:  447 NDECFNV-EGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQS--EIKLXXXXXXXXXAELE----QKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQ 941
            +DE   V +G E+  EN+D+ +      +  S SG+   ++K ++  +R   +EL    S ++ N S  EI++PP P G+ P  L+    +KI ++  +  +   +    I   KE+RNPSIYEKLI++  IDE GTNYP +++D + + E+SYYE LAKAQ   E ++LE+
Sbjct:   44 DDEVSRVKDGEENASENEDSDENDRNSEEDDSESGEPQDELKEVITGERRDPNELVAQFSEKVRNMSPEEIRIPPEPPGRCPGHLQLPGQEKIYKLYERKLHGDFDTNSHIQKKKEFRNPSIYEKLIQYCGIDELGTNYPKDMFDSHGWSEDSYYESLAKAQ-KVEMEKLEK 214
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BLAST of SAP30-binding protein vs. Ensembl Cavefish
Match: sap30bp (SAP30 binding protein [Source:NCBI gene;Acc:103047331])

HSP 1 Score: 72.0182 bits (175), Expect = 8.532e-14
Identity = 55/156 (35.26%), Postives = 86/156 (55.13%), Query Frame = 3 
Query:  447 NDECFNV-EGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAK 905
            +DE   V +G E+  EN+D+ +      +  S SG+   ++K             P   S ++ N S  EI++PP P G+ P  L++KI ++  +  +   +    I   KE+RNPSIYEKLI++  IDE GTNYP +++D + + E+SYYE L K
Sbjct:   44 DDEVSRVKDGEENASENEDSDENDRNSEEDDSESGEPQDELKVF---------GCPSQFSEKVRNMSPEEIRIPPEPPGRCPGHLQEKIYKLYERKLHGDFDTNSHIQKKKEFRNPSIYEKLIQYCGIDELGTNYPKDMFDSHGWSEDSYYESLGK 190
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BLAST of SAP30-binding protein vs. Ensembl Nematostella
Match: EDO37525 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFM7])

HSP 1 Score: 70.8626 bits (172), Expect = 6.370e-14
Identity = 49/125 (39.20%), Postives = 67/125 (53.60%), Query Frame = 3 
Query:  690 LEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWD---TGQSKPQT 1055
            L+ KI+ +  K   +  +    +   K++RNPSIYEKLI +  I E GTNYP EL++P  + +E  YEVLAK Q  A  KR E+         S T  T     + + RK+KWD   TG +K  T
Sbjct:  129 LQDKISNLLEKKYRTNKDWNAAVQKRKDFRNPSIYEKLISYCNIQENGTNYPKELFNPEAWLKEPTYEVLAKQQKEAYEKR-EKAKRTHIEFMSGTKKTGPASDDAKKRKSKWDVSSTGVTKTVT 252
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BLAST of SAP30-binding protein vs. Ensembl Medaka
Match: sap30bp (SAP30 binding protein [Source:NCBI gene;Acc:101155588])

HSP 1 Score: 81.6481 bits (200), Expect = 7.260e-17
Identity = 46/106 (43.40%), Postives = 68/106 (64.15%), Query Frame = 3 
Query:  600 SELPYLLSRQIFNQS--EIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQ 911
             EL  L S ++ N S  EI++P  P G+  ++L++KI ++  +  +   +    I   KE+RNPSIYEKLI+   IDE G NYP +++DP+ + E+SYYE LAKAQ
Sbjct:   95 GELVALFSERVRNMSPDEIRIPSEPPGRCSSQLQEKIHKLYERKLHGDFDTNSHIQKKKEFRNPSIYEKLIQFCAIDELGMNYPKDVFDPHGWSEDSYYEALAKAQ 200
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BLAST of SAP30-binding protein vs. Planmine SMEST
Match: SMESG000069710.1 (SMESG000069710.1)

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 3 
Query:   36 MQSPKNSSKHSLVTYGXXXXXXXXXXXXNIPLPPRELSNDHSNEMRSMDISPITNDTKSNCSNERSPKERNSQVIPLKRRKTNDSNFGGLVSYGMXXXXXXXXXXXXXXXXXXYLTFTTEKDSSTKKDDIQESIPVDNDECFNVEGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQSEIKLXXXXXXXXXAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLXXXXXXXXXXXXXXXIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK 1124
            MQSPKNSSKHSLVTYGSDISDEELDSLSNIPLPPRELSNDHSNEMRSMDISPITNDTKSNCSNERSPKERNSQVIPLKRRKTNDSNFGGLVSYGMDEDDNFDEDDDDENDSENYLTFTTEKDSSTKKDDIQESIPVDNDECFNVEGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQSEIKLPPPPMGKPPAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLSSGTATKPSKTATTKIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK
Sbjct:    1 MQSPKNSSKHSLVTYGSDISDEELDSLSNIPLPPRELSNDHSNEMRSMDISPITNDTKSNCSNERSPKERNSQVIPLKRRKTNDSNFGGLVSYGMDEDDNFDEDDDDENDSENYLTFTTEKDSSTKKDDIQESIPVDNDECFNVEGRESDIENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIFNQSEIKLPPPPMGKPPAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNPSIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRLEQLSSGTATKPSKTATTKIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQVVIETKVMFATHK 363
The following BLAST results are available for this feature:
BLAST of SAP30-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SAP30BP1.313e-1648.31SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC... [more]
SAP30BP1.594e-1645.71SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC... [more]
SAP30BP1.751e-1648.31SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC... [more]
SAP30BP2.248e-1645.71SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC... [more]
SAP30BP3.085e-1645.71SAP30 binding protein [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of SAP30-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Y67D2.72.588e-735.82pep chromosome:WBcel235:III:1555395:1558529:-1 gen... [more]
Y67D2.72.588e-735.82pep chromosome:WBcel235:III:1555410:1558529:-1 gen... [more]
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BLAST of SAP30-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG20633.421e-2144.83gene:FBgn0033400 transcript:FBtr0088600[more]
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BLAST of SAP30-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sap30bp6.983e-1838.51SAP30 binding protein [Source:ZFIN;Acc:ZDB-GENE-03... [more]
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BLAST of SAP30-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SAP30BP7.752e-1952.70SAP30 binding protein [Source:NCBI gene;Acc:100379... [more]
SAP30BP2.706e-1852.70SAP30 binding protein [Source:NCBI gene;Acc:100379... [more]
SAP30BP3.487e-1852.70SAP30 binding protein [Source:NCBI gene;Acc:100379... [more]
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BLAST of SAP30-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sap30bp1.974e-1645.71SAP30 binding protein [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of SAP30-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q02614|S30BP_MOUSE1.381e-1545.71SAP30-binding protein OS=Mus musculus OX=10090 GN=... [more]
sp|Q9UHR5|S30BP_HUMAN1.784e-1545.71SAP30-binding protein OS=Homo sapiens OX=9606 GN=S... [more]
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BLAST of SAP30-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A069DR341.022e-1933.19Putative transcriptional regulator OS=Panstrongylu... [more]
A0A0V0G7051.032e-1933.19Putative transcriptional regulator OS=Triatoma dim... [more]
A0A224XHY41.129e-1933.19Putative transcriptional regulator OS=Panstrongylu... [more]
A0A182ITX01.319e-1944.35Uncharacterized protein OS=Anopheles atroparvus OX... [more]
U5EGX11.413e-1939.49Putative transcriptional regulator protein hcngp O... [more]
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BLAST of SAP30-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sap30bp7.724e-1937.50SAP30 binding protein [Source:NCBI gene;Acc:103047... [more]
sap30bp7.124e-1736.63SAP30 binding protein [Source:NCBI gene;Acc:103047... [more]
sap30bp8.532e-1435.26SAP30 binding protein [Source:NCBI gene;Acc:103047... [more]
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BLAST of SAP30-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAP30-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAP30-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO375256.370e-1439.20Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SAP30-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sap30bp7.260e-1743.40SAP30 binding protein [Source:NCBI gene;Acc:101155... [more]
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BLAST of SAP30-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000069710.10.000e+0100.00SMESG000069710.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025974 ID=SMED30025974|Name=SAP30-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1176bp
AGAAGATTCAAATAATACAATTTTTATTTTAGATTATGCAGTCACCTAAA
AATTCTAGTAAACATTCGCTAGTTACTTATGGTTCAGATATATCAGACGA
AGAGTTAGATAGTCTGAGTAATATACCTTTGCCACCACGTGAACTATCAA
ACGATCATTCGAATGAAATGCGATCAATGGATATTAGCCCAATAACAAAT
GATACAAAATCAAATTGCTCTAATGAACGTTCACCAAAAGAAAGAAACTC
ACAAGTTATTCCCTTAAAAAGAAGAAAAACTAATGATAGTAATTTCGGTG
GTCTTGTTTCATATGGTATGGATGAGGATGACAATTTTGACGAGGACGAC
GACGATGAGAATGACAGTGAAAATTATTTAACGTTTACCACTGAAAAAGA
TTCTTCAACAAAAAAAGATGATATTCAAGAATCGATTCCTGTTGACAATG
ACGAATGCTTTAACGTAGAAGGTCGCGAATCTGATATTGAAAATGACGAT
ACTCACGACCGTCCACCTCGAGATGTTGACCTGAAAAGTCGAAGTGGTCA
ATCATCTTCTCAAGTCAAAGCAATCGTTATGACCAAGCGCCCAAAAGGAA
GTGAATTGCCTTATCTGCTGTCCCGTCAGATATTCAACCAATCAGAAATC
AAATTGCCTCCACCACCGATGGGCAAACCTCCAGCTGAGCTTGAACAAAA
GATTACTGAAATGCGTGCAAAGGCCAAAAACTCTCGCTGTAACATTGCAC
AATTGATTTTGAGCAATAAAGAATATCGAAACCCGAGCATTTATGAGAAA
TTAATCGAGCATATGAAAATCGATGAGAGGGGGACAAATTATCCACCGGA
ACTTTATGATCCGTATCGATTCGGAGAGGAATCCTATTACGAGGTGCTCG
CCAAGGCCCAGGATGCGGCAGAACGTAAGCGGTTGGAGCAGCTATCAAGC
GGAACCGCTACAAAACCAAGCAAGACCGCAACAACAAAAATTATCGATTC
CGAATTAAGGATGCGAAAGACGAAATGGGACACGGGACAATCGAAACCTC
AAACGTCTAAATCCCACTCAAGCGGTTCCACTCAAGTTGTCATTGAGACT
AAGGTTATGTTTGCTACTCACAAATAAATTATTTCTTGTTTTGTTGTTTA
TTAATAAATTATTATAATGATTAAAA
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protein sequence of SMED30025974-orf-1

>SMED30025974-orf-1 ID=SMED30025974-orf-1|Name=SMED30025974-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=364bp
MQSPKNSSKHSLVTYGSDISDEELDSLSNIPLPPRELSNDHSNEMRSMDI
SPITNDTKSNCSNERSPKERNSQVIPLKRRKTNDSNFGGLVSYGMDEDDN
FDEDDDDENDSENYLTFTTEKDSSTKKDDIQESIPVDNDECFNVEGRESD
IENDDTHDRPPRDVDLKSRSGQSSSQVKAIVMTKRPKGSELPYLLSRQIF
NQSEIKLPPPPMGKPPAELEQKITEMRAKAKNSRCNIAQLILSNKEYRNP
SIYEKLIEHMKIDERGTNYPPELYDPYRFGEESYYEVLAKAQDAAERKRL
EQLSSGTATKPSKTATTKIIDSELRMRKTKWDTGQSKPQTSKSHSSGSTQ
VVIETKVMFATHK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR012479SAP30BP
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012479SAP30-binding proteinPFAMPF07818HCNGPcoord: 207..299
e-value: 6.6E-26
score: 90.8
IPR012479SAP30-binding proteinPANTHERPTHR13464TRANSCRIPTIONAL REGULATOR PROTEIN HCNGPcoord: 5..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..319
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..178
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15