Four and A half lim domains
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025952 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 402.519 bits (1033), Expect = 4.662e-133 Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3 Query: 123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 + H WR CV C+ H L + V N + Q + + + + W+P GL + ++ +++ ++ N+ P + + R+ + Q+P D +S H + E K+ + F +R+ L G + N+ + C C E ++ I H +CF C+ C++ LVD YC ++ I+C RH+ EL RC+ CDELI ++TKAM++++H +HF C CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I DSKDLS+K+ HWHE CFLC +C SLVD F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C +IGTKSFIP N + C CYE K A +C KC ++I G+TYK +PWH+ECF CT+C L+ +FTSK+EKPYC C+ +LF KRC CT+PI+ G GG KFIS D HWH +CF C++C S+VGKGF +++C C AR Sbjct: 43 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 558
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 402.519 bits (1033), Expect = 4.662e-133 Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3 Query: 123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 + H WR CV C+ H L + V N + Q + + + + W+P GL + ++ +++ ++ N+ P + + R+ + Q+P D +S H + E K+ + F +R+ L G + N+ + C C E ++ I H +CF C+ C++ LVD YC ++ I+C RH+ EL RC+ CDELI ++TKAM++++H +HF C CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I DSKDLS+K+ HWHE CFLC +C SLVD F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C +IGTKSFIP N + C CYE K A +C KC ++I G+TYK +PWH+ECF CT+C L+ +FTSK+EKPYC C+ +LF KRC CT+PI+ G GG KFIS D HWH +CF C++C S+VGKGF +++C C AR Sbjct: 43 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 558
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 403.675 bits (1036), Expect = 2.185e-132 Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3 Query: 123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 + H WR CV C+ H L + V N + Q + + + + W+P GL + ++ +++ ++ N+ P + + R+ + Q+P D +S H + E K+ + F +R+ L G + N+ + C C E ++ I H +CF C+ C++ LVD YC ++ I+C RH+ EL RC+ CDELI ++TKAM++++H +HF C CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I DSKDLS+K+ HWHE CFLC +C SLVD F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C +IGTKSFIP N + C CYE K A +C KC ++I G+TYK +PWH+ECF CT+C L+ +FTSK+EKPYC C+ +LF KRC CT+PI+ G GG KFIS D HWH +CF C++C S+VGKGF +++C C AR Sbjct: 135 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 650
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 401.364 bits (1030), Expect = 5.502e-132 Identity = 199/486 (40.95%), Postives = 293/486 (60.29%), Query Frame = 3 Query: 300 ISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 + + + W+P GL + ++ +++ ++ N+ P + + R+ + Q+P D +S H + E K+ + F +R+ L G + N+ + C C E ++ I H +CF C+ C++ LVD YC ++ I+C RH+ EL RC+ CDELI ++TKAM++++H +HF C CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I DSKDLS+K+ HWHE CFLC +C SLVD F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C +IGTKSFIP N + C CYE K A +C KC ++I G+TYK +PWH+ECF CT+C L+ +FTSK+EKPYC C+ +LF KRC CT+PI+ G GG KFIS D HWH +CF C++C S+VGKGF +++C C AR Sbjct: 149 VPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 613
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 404.445 bits (1038), Expect = 2.347e-131 Identity = 210/546 (38.46%), Postives = 310/546 (56.78%), Query Frame = 3 Query: 123 YTNHKWRFRCVSCRCPNYRH-VSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 + H WR CV C+ H + +L+ + G S E + + + W+P GL + ++ +++ ++ N+ P + + R+ + Q+P D +S H + E K+ + F +R+ L G + N+ + C C E ++ I H +CF C+ C++ LVD YC ++ I+C RH+ EL RC+ CDELI ++TKAM++++H +HF C CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I DSKDLS+K+ HWHE CFLC +C SLVD F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C +IGTKSFIP N + C CYE K A +C KC ++I G+TYK +PWH+ECF CT+C L+ +FTSK+EKPYC C+ +LF KRC CT+PI+ G GG KFIS D HWH +CF C++C S+VGKGF +++C C AR Sbjct: 242 WKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPE---------VPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 757
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302779) HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138 Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3 Query: 90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 + C+ CKS C + H WR C SC+CP H + T H +L + A S E+ +L W+P G++ + + F ++ ++ P E R+ I YQ+P D +S E H + + E F +R+ L I+ E C+ CD + ++ H CF C TC LVD YC +D ++C RH+ E+ RCA CDELI + ++TKAMD+++H HF C CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I DSKDLS+K+ HWHE CFLC CH SLVD+ F K + +YC NC+D +F+S CDGCG++FR G KK EY +QWHE+CF C C +IGTKSFIP +I C GCYE K A +C KC+++I G+TYK +PWH+ECF CT C L+ +FTS++EKPYC ECF ELF KRC C +PI+ G GG +FIS D HWH +CF C+ C+AS+VG+GF ++C C Sbjct: 38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554 HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40 Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 CA CD I + A + +H + F C C+S L Y + DD +C + + C C E I + + WH F C C +SL + + +D++ YC C++ F++ C+ C KI K Y K WHE CF+C C+ S+ K F KI C CY+ + A +C C E+ R + YK WH+ CF C C+ + F +E++ YC C+ E F RC KC + I+ GG ++ ++ WH ECF C+ C ++ G+ F ++++ C C G Sbjct: 193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492 HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25 Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H +CF C C LVD + D I+C + RC C E+ + TK M+ +++H F C VC +++ +I R+ +C C+E +F C KC++ I+ S +++K WH +CF C C+ +L + FT++D YC CF E F+ C C K G + + + WH DCF+CA C S+ + FI I C C + K Sbjct: 340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175 H +CF C CK + + + I+C + E RC +C+++I + T ++ +H E F C C+ +L G ++ RD+ P+C +CF F C C + I+ ++ +SF++ HWH CF+C C SLV R F T + CP+C +K Sbjct: 401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558 HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16 Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C+ CN + ++ + K+ C Y +C C E+I T D WH F C C + L+ ++ +++ PYC +C+ +F C++C + I D+K L S D HWH CF C KC S+V K F + D++ CG+C A+ Sbjct: 218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302781) HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138 Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3 Query: 90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 + C+ CKS C + H WR C SC+CP H + T H +L + A S E+ +L W+P G++ + + F ++ ++ P E R+ I YQ+P D +S E H + + E F +R+ L I+ E C+ CD + ++ H CF C TC LVD YC +D ++C RH+ E+ RCA CDELI + ++TKAMD+++H HF C CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I DSKDLS+K+ HWHE CFLC CH SLVD+ F K + +YC NC+D +F+S CDGCG++FR G KK EY +QWHE+CF C C +IGTKSFIP +I C GCYE K A +C KC+++I G+TYK +PWH+ECF CT C L+ +FTS++EKPYC ECF ELF KRC C +PI+ G GG +FIS D HWH +CF C+ C+AS+VG+GF ++C C Sbjct: 38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554 HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40 Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 CA CD I + A + +H + F C C+S L Y + DD +C + + C C E I + + WH F C C +SL + + +D++ YC C++ F++ C+ C KI K Y K WHE CF+C C+ S+ K F KI C CY+ + A +C C E+ R + YK WH+ CF C C+ + F +E++ YC C+ E F RC KC + I+ GG ++ ++ WH ECF C+ C ++ G+ F ++++ C C G Sbjct: 193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492 HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25 Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H +CF C C LVD + D I+C + RC C E+ + TK M+ +++H F C VC +++ +I R+ +C C+E +F C KC++ I+ S +++K WH +CF C C+ +L + FT++D YC CF E F+ C C K G + + + WH DCF+CA C S+ + FI I C C + K Sbjct: 340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175 H +CF C CK + + + I+C + E RC +C+++I + T ++ +H E F C C+ +L G ++ RD+ P+C +CF F C C + I+ ++ +SF++ HWH CF+C C SLV R F T + CP+C +K Sbjct: 401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558 HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16 Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C+ CN + ++ + K+ C Y +C C E+I T D WH F C C + L+ ++ +++ PYC +C+ +F C++C + I D+K L S D HWH CF C KC S+V K F + D++ CG+C A+ Sbjct: 218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0306704) HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138 Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3 Query: 90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 + C+ CKS C + H WR C SC+CP H + T H +L + A S E+ +L W+P G++ + + F ++ ++ P E R+ I YQ+P D +S E H + + E F +R+ L I+ E C+ CD + ++ H CF C TC LVD YC +D ++C RH+ E+ RCA CDELI + ++TKAMD+++H HF C CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I DSKDLS+K+ HWHE CFLC CH SLVD+ F K + +YC NC+D +F+S CDGCG++FR G KK EY +QWHE+CF C C +IGTKSFIP +I C GCYE K A +C KC+++I G+TYK +PWH+ECF CT C L+ +FTS++EKPYC ECF ELF KRC C +PI+ G GG +FIS D HWH +CF C+ C+AS+VG+GF ++C C Sbjct: 38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554 HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40 Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 CA CD I + A + +H + F C C+S L Y + DD +C + + C C E I + + WH F C C +SL + + +D++ YC C++ F++ C+ C KI K Y K WHE CF+C C+ S+ K F KI C CY+ + A +C C E+ R + YK WH+ CF C C+ + F +E++ YC C+ E F RC KC + I+ GG ++ ++ WH ECF C+ C ++ G+ F ++++ C C G Sbjct: 193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492 HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25 Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H +CF C C LVD + D I+C + RC C E+ + TK M+ +++H F C VC +++ +I R+ +C C+E +F C KC++ I+ S +++K WH +CF C C+ +L + FT++D YC CF E F+ C C K G + + + WH DCF+CA C S+ + FI I C C + K Sbjct: 340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175 H +CF C CK + + + I+C + E RC +C+++I + T ++ +H E F C C+ +L G ++ RD+ P+C +CF F C C + I+ ++ +SF++ HWH CF+C C SLV R F T + CP+C +K Sbjct: 401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558 HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16 Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C+ CN + ++ + K+ C Y +C C E+I T D WH F C C + L+ ++ +++ PYC +C+ +F C++C + I D+K L S D HWH CF C KC S+V K F + D++ CG+C A+ Sbjct: 218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0332703) HSP 1 Score: 406.757 bits (1044), Expect = 4.333e-135 Identity = 219/540 (40.56%), Postives = 302/540 (55.93%), Query Frame = 3 Query: 141 RFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 R C SC+CP H + T H +L + A S E+ +L W+P G++ + + F ++ ++ P E R+ I YQ+P D +S E H + + E F +R+ L I+ E C+ CD + ++ H CF C TC LVD YC +D ++C RH+ E+ RCA CDELI + ++TKAMD+++H HF C CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I DSKDLS+K+ HWHE CFLC CH SLVD+ F K + +YC NC+D +F+S CDGCG++FR G KK EY +QWHE+CF C C +IGTKSFIP +I C GCYE K A +C KC+++I G+TYK +PWH+ECF CT C L+ +FTS++EKPYC ECF ELF KRC C +PI+ G GG +FIS D HWH +CF C+ C+AS+VG+GF ++C C Sbjct: 26 RKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 524 HSP 2 Score: 156.377 bits (394), Expect = 1.150e-40 Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 CA CD I + A + +H + F C C+S L Y + DD +C + + C C E I + + WH F C C +SL + + +D++ YC C++ F++ C+ C KI K Y K WHE CF+C C+ S+ K F KI C CY+ + A +C C E+ R + YK WH+ CF C C+ + F +E++ YC C+ E F RC KC + I+ GG ++ ++ WH ECF C+ C ++ G+ F ++++ C C G Sbjct: 163 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 462 HSP 3 Score: 110.923 bits (276), Expect = 2.282e-25 Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H +CF C C LVD + D I+C + RC C E+ + TK M+ +++H F C VC +++ +I R+ +C C+E +F C KC++ I+ S +++K WH +CF C C+ +L + FT++D YC CF E F+ C C K G + + + WH DCF+CA C S+ + FI I C C + K Sbjct: 310 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 527 HSP 4 Score: 85.8853 bits (211), Expect = 2.727e-17 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175 H +CF C CK + + + I+C + E RC +C+++I + T ++ +H E F C C+ +L G ++ RD+ P+C +CF F C C + I+ ++ +SF++ HWH CF+C C SLV R F T + CP+C +K Sbjct: 371 HENCFCCCVCKTAIGTKSF--IPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 528 HSP 5 Score: 84.3445 bits (207), Expect = 8.501e-17 Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C+ CN + ++ + K+ C Y +C C E+I T D WH F C C + L+ ++ +++ PYC +C+ +F C++C + I D+K L S D HWH CF C KC S+V K F + D++ CG+C A+ Sbjct: 188 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 344
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302780) HSP 1 Score: 405.601 bits (1041), Expect = 2.654e-134 Identity = 219/540 (40.56%), Postives = 302/540 (55.93%), Query Frame = 3 Query: 141 RFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 R C SC+CP H + T H +L + A S E+ +L W+P G++ + + F ++ ++ P E R+ I YQ+P D +S E H + + E F +R+ L I+ E C+ CD + ++ H CF C TC LVD YC +D ++C RH+ E+ RCA CDELI + ++TKAMD+++H HF C CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I DSKDLS+K+ HWHE CFLC CH SLVD+ F K + +YC NC+D +F+S CDGCG++FR G KK EY +QWHE+CF C C +IGTKSFIP +I C GCYE K A +C KC+++I G+TYK +PWH+ECF CT C L+ +FTS++EKPYC ECF ELF KRC C +PI+ G GG +FIS D HWH +CF C+ C+AS+VG+GF ++C C Sbjct: 55 RKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEG----ACSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 553 HSP 2 Score: 157.147 bits (396), Expect = 9.940e-41 Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 CA CD I + A + +H + F C C+S L Y + DD +C + + C C E I + + WH F C C +SL + + +D++ YC C++ F++ C+ C KI K Y K WHE CF+C C+ S+ K F KI C CY+ + A +C C E+ R + YK WH+ CF C C+ + F +E++ YC C+ E F RC KC + I+ GG ++ ++ WH ECF C+ C ++ G+ F ++++ C C G Sbjct: 192 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 491 HSP 3 Score: 110.923 bits (276), Expect = 3.570e-25 Identity = 71/224 (31.70%), Postives = 108/224 (48.21%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H +CF C C LVD + D I+C + RC C E V + TK M+ +++H F C VC +++ +I R+ +C C+E +F C KC++ I+ S +++K WH +CF C C+ +L + FT++D YC CF E F+ C C K G + + + WH DCF+CA C S+ + FI I C C + K Sbjct: 339 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGE--VFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 556 HSP 4 Score: 85.5001 bits (210), Expect = 3.991e-17 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175 H +CF C CK + + + I+C + E RC +C+++I + T ++ +H E F C C+ +L G ++ RD+ P+C +CF F C C + I+ ++ +SF++ HWH CF+C C SLV R F T + CP+C +K Sbjct: 400 HENCFCCCVCKTAIGTKSF--IPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 557 HSP 5 Score: 85.1149 bits (209), Expect = 5.149e-17 Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C+ CN + ++ + K+ C Y +C C E+I T D WH F C C + L+ ++ +++ PYC +C+ +F C++C + I D+K L S D HWH CF C KC S+V K F + D++ CG+C A+ Sbjct: 217 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 373
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93 Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C CH SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C +C + I G+TY PWHK+CFLCT C++ LS +FTS+++ PYC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276 HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33 Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y +++ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + C +C + I S+ + K WHE CF C C + +SF KDN YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23 Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D++ +CV C+E QF C +C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93 Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C CH SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C +C + I G+TY PWHK+CFLCT C++ LS +FTS+++ PYC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276 HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33 Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y +++ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + C +C + I S+ + K WHE CF C C + +SF KDN YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23 Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D++ +CV C+E QF C +C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93 Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C CH SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C +C + I G+TY PWHK+CFLCT C++ LS +FTS+++ PYC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276 HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33 Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y +++ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + C +C + I S+ + K WHE CF C C + +SF KDN YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23 Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D++ +CV C+E QF C +C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 284.263 bits (726), Expect = 5.968e-91 Identity = 135/285 (47.37%), Postives = 177/285 (62.11%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LR+DNP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE CF C C SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C C + I G+TY PWHK+CFLCT C++ LS +FTS+++ YC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF D ++C CG Sbjct: 2 TERYDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECG 276 HSP 2 Score: 137.117 bits (344), Expect = 7.817e-36 Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y D+ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + CH+C + I S+ + K WHE CF C C + +SF KDN+ YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F + + C EC Sbjct: 5 YDCHYCKESLFGKKYVLREDNP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 103.219 bits (256), Expect = 6.205e-24 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C CK LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D++ +CV C+E QF C C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEDCFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 284.263 bits (726), Expect = 5.968e-91 Identity = 135/285 (47.37%), Postives = 177/285 (62.11%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LR+DNP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE CF C C SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C C + I G+TY PWHK+CFLCT C++ LS +FTS+++ YC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF D ++C CG Sbjct: 2 TERYDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECG 276 HSP 2 Score: 137.117 bits (344), Expect = 7.817e-36 Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y D+ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + CH+C + I S+ + K WHE CF C C + +SF KDN+ YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F + + C EC Sbjct: 5 YDCHYCKESLFGKKYVLREDNP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 103.219 bits (256), Expect = 6.205e-24 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C CK LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D++ +CV C+E QF C C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEDCFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl2 (four and a half LIM domains 2 [Source:NCBI gene;Acc:100145283]) HSP 1 Score: 293.893 bits (751), Expect = 1.574e-94 Identity = 138/285 (48.42%), Postives = 181/285 (63.51%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C SL G KY+LR++NP+CVKC+E+ + N+C +C + I CD KDLS+K+ HWHE CF C C SLVD+ F KD +L C C+ ++SS C C K G +K EY G WHE CFIC+ C Q IGTKSFIP + + C+ CYE + A C +C + I G+TY+ PWHKECF+CT C+K LS +FTS++E YC CF L+ K+C CT PIS G GG K+IS + WH++CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPIS----------GLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPECG 276 HSP 2 Score: 130.568 bits (327), Expect = 2.239e-33 Identity = 80/276 (28.99%), Postives = 134/276 (48.55%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C CKE L Y ++ +C++ + L C EC + I KD + D+ +H F C C SL + +D++ C +C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++F+ C C K TG Y + WH++CFIC C + + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 FDCHYCKESLFGKKYLLREENP--YCVKCYESLYSNSCEECKKPIGCDGKDLS-YKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTG--GVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275 HSP 3 Score: 107.457 bits (267), Expect = 2.893e-25 Identity = 66/220 (30.00%), Postives = 107/220 (48.64%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H SCF C CK+ LVD + + + + C + +C+EC + I+ T+ M+ +H F C C + +I +D+ FCV C+E QF C +C + I+ + +++++ WH++CF+C C L + FT++D + YC NCF ++ C GC G K + +QWH DCF C C S+ + F+ I C C Sbjct: 62 HESCFHCFQCKKSLVDKPF--AAKDEHLICTECYSNEYSSKCSECKKTIMPG--TRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAIT--TGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl5 (four and a half LIM domains 5 [Source:NCBI gene;Acc:100144736]) HSP 1 Score: 272.707 bits (696), Expect = 2.343e-86 Identity = 131/286 (45.80%), Postives = 176/286 (61.54%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748 E F C C SL G KY L+DD P+C+KCF + F N C +C + I C+SKDL++K+ HWHE CF CD C SLV++ F KD L C C+ ++SS C GC G +K EY G WHE CF+C C + +G K FIP KI C+ CYE + A +C C + I K G++++ WH+ECF+CTSC+K L K TS++E PYC +CF L+ K+C C +PI+ G GG K+IS D WH++CF C+KC S+VG+ F ED ++C SCG A Sbjct: 4 EIFDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPIT----------GQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCGLA 279 HSP 2 Score: 135.961 bits (341), Expect = 3.647e-35 Identity = 77/279 (27.60%), Postives = 135/279 (48.39%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C CKE L Y +D +C++ F L C C + I N D +H F C CD SL + +D+ C++C+ ++ + C C I S+ + + +WHE CF+C C + + ++ F K++ +YC C++++F++ C C K G + +QWH +CF+C C +++ + + C+ C++N +A+KC C++ I G I+++ WH +CF C C K L KF + E+ C C + + Sbjct: 6 FDCFHCKESLYGKKYTLKDD--IPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKG--GLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCGLAI 280 HSP 3 Score: 85.8853 bits (211), Expect = 6.186e-18 Identity = 50/156 (32.05%), Postives = 85/156 (54.49%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCD--SKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160 H +CF C++C+E + + + +SK I+C+ + + +C C + I + +Q++H E F C C +L G K RD++P+CV CF+N + C C++ I+ +K +SF++ WH CF C C SLV F T ++ + CP+C Sbjct: 124 HETCFVCQSCREPVGNKPF-IPKESK-IYCMPCYEKQFANQCKSCRKAITKGGLS-FQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: shroom3 (shroom family member 3 [Source:Xenbase;Acc:XB-GENE-490224]) HSP 1 Score: 265.774 bits (678), Expect = 9.051e-84 Identity = 129/283 (45.58%), Postives = 178/283 (62.90%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 E F C C SL G KYI ++ P+C+ C+++ F NTC +C E I D ++L +++ H+HE CF C C SL D FT +D L C +C+ +FSS C C K G +K EY G+ WHE CFIC C Q IG++SFIP N CI CYE+K A +C C + + K G+TY+ +PWHKECF+CT C+ L+ +FTS++EKPYC +CF L+ K+C CT+PI+ GFGG K++S + HWH CFNCS+C S+VGKGF + ++C +C Sbjct: 3 EPFDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPIT----------GFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275 HSP 2 Score: 127.487 bits (319), Expect = 3.251e-32 Identity = 81/279 (29.03%), Postives = 126/279 (45.16%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNK-DFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C +CKE L Y + +C+ + L C EC ELI + D+ +H F C CD SL + +D+ C C+ N+F + C C + + S+ L + WHE CF+C+ C + RSF ++ YC C++ K + C C K G Y + WH++CF+C C + + F + K CI C+ N +A+KC C++ I G ++++ WH CF C+ C L F E C C +L Sbjct: 5 FDCDSCKESLYGRKYIQMEEGP--YCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKG--GVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRACNSDL 279 HSP 3 Score: 118.242 bits (295), Expect = 5.253e-29 Identity = 66/224 (29.46%), Postives = 106/224 (47.32%), Query Frame = 3 Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 F CD C +SL R + + YC C+D F++ CD C ++ ++ Y + +HE CF C C+ S+ + F + ++ C CY N+ + KC C + + + Y G WH+ CF+C SCQ+ + F + + YC C+ RC C K L KG ++ D+ WH ECF C+ C+ + G+ F Q+++ C C G Sbjct: 5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCK---------KSLTKGG-----VTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFG 214
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 223.016 bits (567), Expect = 3.703e-67 Identity = 110/286 (38.46%), Postives = 155/286 (54.20%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C + L G KYI +D + CVKCF+ NTC +C + I DSK+L +K +WH+ CF C C+ L + F KDN + C C + S C GC K + G + EY G WHE+CF C++C Q+IG+ SF P + C+ C+E K A+ C KC+ I GITY+ PWH +CF+C +C K L+ +FT+ E+ YC +C+ K+C C PI+ GFG G ++ + WH CF C KC ++ K F +Q+ C C Sbjct: 18 AERFDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPIT----------GFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCA 293 HSP 2 Score: 126.716 bits (317), Expect = 9.022e-32 Identity = 77/280 (27.50%), Postives = 131/280 (46.79%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C C+ L Y + C++ F ++ CAEC + I V+ ++ +H F C C L ++I +D+ C KC + C C ++I +++ +K WHE+CF C C ++ SF K +YC C ++KF+ C C +G Y+ + WH DCF+C C++ + + F + C+ CY++ A+KC C+ I G + Y+G+ WH+ CF C C L+ +F E+ YC +C ++ Sbjct: 21 FDCHYCRAPLQGKKYIEKDGHNT--CVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQ--DQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 296 HSP 3 Score: 65.4698 bits (158), Expect = 5.780e-11 Identity = 46/183 (25.14%), Postives = 79/183 (43.17%), Query Frame = 3 Query: 621 CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKD---LSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160 CS C KQ + R A H CF C CK+ + + ++C+ + C +C+ I + T DQ +H + F C C L G ++ +D+ +CV C+++ C C+ I+ K ++++ WHE CF C C +L ++ F + +YC +C Sbjct: 117 CSGCHKQIQPGG----RNVEYKGSAWHEECFTCSNCKQAIGSGSF--FPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGIT-YQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 292
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 222.631 bits (566), Expect = 4.450e-67 Identity = 110/286 (38.46%), Postives = 155/286 (54.20%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C + L G KYI +D + CVKCF+ NTC +C + I DSK+L +K +WH+ CF C C+ L + F KDN + C C + S C GC K + G + EY G WHE+CF C++C Q+IG+ SF P + C+ C+E K A+ C KC+ I GITY+ PWH +CF+C +C K L+ +FT+ E+ YC +C+ K+C C PI+ GFG G ++ + WH CF C KC ++ K F +Q+ C C Sbjct: 13 AERFDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPIT----------GFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCA 288 HSP 2 Score: 126.331 bits (316), Expect = 9.150e-32 Identity = 77/280 (27.50%), Postives = 131/280 (46.79%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C C+ L Y + C++ F ++ CAEC + I V+ ++ +H F C C L ++I +D+ C KC + C C ++I +++ +K WHE+CF C C ++ SF K +YC C ++KF+ C C +G Y+ + WH DCF+C C++ + + F + C+ CY++ A+KC C+ I G + Y+G+ WH+ CF C C L+ +F E+ YC +C ++ Sbjct: 16 FDCHYCRAPLQGKKYIEKDGHNT--CVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQ--DQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 291 HSP 3 Score: 65.4698 bits (158), Expect = 4.578e-11 Identity = 46/183 (25.14%), Postives = 79/183 (43.17%), Query Frame = 3 Query: 621 CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKD---LSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160 CS C KQ + R A H CF C CK+ + + ++C+ + C +C+ I + T DQ +H + F C C L G ++ +D+ +CV C+++ C C+ I+ K ++++ WHE CF C C +L ++ F + +YC +C Sbjct: 112 CSGCHKQIQPGG----RNVEYKGSAWHEECFTCSNCKQAIGSGSF--FPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGIT-YQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 287
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 290.812 bits (743), Expect = 1.662e-93 Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYIL+++NP CV CFE + NTC +C I CD KDLS+K+ HWHE CF C C SLVD+ F K+ L C +C+ ++SS C C K G +K EY G WHE CF C C Q IGTKSFIP + C+ CYE ++A +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT+++E PYC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 119.013 bits (297), Expect = 2.125e-29 Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ C+ F EL C EC I KD + D+ +H F C C SSL + +++ C C+ N++ + C +C + I ++ + +K WHE CF C C + +SF K+N +C C++++++ C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 103.99 bits (258), Expect = 3.041e-24 Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + + + + C + +C EC + I+ T+ M+ +H F C C + +I +++ FCV C+E Q+ C +C + I+ + ++++E WH++CF+C C L + FT +D + YC CF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 290.812 bits (743), Expect = 1.662e-93 Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYIL+++NP CV CFE + NTC +C I CD KDLS+K+ HWHE CF C C SLVD+ F K+ L C +C+ ++SS C C K G +K EY G WHE CF C C Q IGTKSFIP + C+ CYE ++A +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT+++E PYC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 119.013 bits (297), Expect = 2.125e-29 Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ C+ F EL C EC I KD + D+ +H F C C SSL + +++ C C+ N++ + C +C + I ++ + +K WHE CF C C + +SF K+N +C C++++++ C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 103.99 bits (258), Expect = 3.041e-24 Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + + + + C + +C EC + I+ T+ M+ +H F C C + +I +++ FCV C+E Q+ C +C + I+ + ++++E WH++CF+C C L + FT +D + YC CF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092]) HSP 1 Score: 270.396 bits (690), Expect = 1.261e-85 Identity = 125/286 (43.71%), Postives = 177/286 (61.89%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748 E F C C+ SL G KYI D P+CV C++N F NTC +C + I DS++L +++ H+HE CF C C SL D FT +D+ L C C+ FSS C CG+ G +K EY G+ WHE CF+C+ C Q +G++SF+P G C+ CYENK A +C +CS+ + + G+TY+ PWH+EC +CT C+ L+ +FTS+++ PYC CF ELF +C C +PI+ G GG K++S D HWH CF+C++C S+VG+GF DQ++C C A Sbjct: 3 EAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPIT----------GLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278 HSP 2 Score: 119.783 bits (299), Expect = 1.315e-29 Identity = 76/275 (27.64%), Postives = 110/275 (40.00%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ +C+ + CAEC +LI + D+ FH F C C SL + +D C +C+ F + C C E + S+ L + WHE CFLC C L RSF YC C++ KF+ C C K G Y + WH +C +C C + + F + C+ C+ A KC C I G ++++ WH CF C C L F ++ C C Sbjct: 5 FDCAKCNESLYGRKYIQTDSGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092]) HSP 1 Score: 263.077 bits (671), Expect = 1.018e-82 Identity = 124/286 (43.36%), Postives = 176/286 (61.54%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748 E F C C+ SL G KYI D P+CV C++N F NTC +C + I DS++L +++ H+HE CF C C SL D FT +D+ L C C+ FSS C CG+ G +K EY G+ WHE CF+C+ C Q +G++SF+P G C+ CYENK A +C +CS+ + + G+TY+ PWH+EC +CT C+ L+ +FTS+++ PYC CF ELF +C C +PI+ GG K++S D HWH CF+C++C S+VG+GF DQ++C C A Sbjct: 3 EAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGG-KYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 287 HSP 2 Score: 113.62 bits (283), Expect = 1.959e-27 Identity = 75/284 (26.41%), Postives = 109/284 (38.38%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-------------ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ +C+ + CAEC +LI + D+ FH F C C SL + +D C +C+ F + C C E + S+ L + WHE CFLC C L RSF YC C++ KF+ C C K G Y + WH +C +C C + + F + C+ C+ A KC C I ++++ WH CF C C L F ++ C C Sbjct: 5 FDCAKCNESLYGRKYIQTDSGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 284
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192]) HSP 1 Score: 256.914 bits (655), Expect = 2.302e-80 Identity = 125/290 (43.10%), Postives = 169/290 (58.28%), Query Frame = 3 Query: 894 HFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDG 1763 F C C SSL G KY+L+DDN +C+ C++ F N C +C E I DSKDL +K HWHE CF C+ CH SLV++ F KD+ L C +C+ + SS C C + G +K E+ G WHE CF+C C Q IGTK I C+ C+E + A C C ++I GIT++ WHKECFLC+ C+K L E F SK++ P+C +C+ L+ K+C CT+PI+ G G KFI D WH+ECFNC KC S+VG+GF +++C CG + D Sbjct: 5 QFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPIT----------GLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA 284 HSP 2 Score: 121.324 bits (303), Expect = 3.540e-30 Identity = 74/276 (26.81%), Postives = 136/276 (49.28%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR----KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C+ C L+ Y +D+ ++C+ + + C +C E I + KD ++ +H F C+ C SL ++ +DD C C+ N+ + C C I S+ + FK +WHE CF+C+ C + + +K++ YC CF+++F+ C+ C K+ +G + + WH++CF+C+ C + + ++F+ + C+ CY + +A+KC C++ I I ++ WH ECF C C L F + + C +C Sbjct: 6 FDCQYCTSSLIGKKYVLKDDN--LYCISCYDRIFSNYCEQCKEPIESDSKDLCYK-NRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276 HSP 3 Score: 61.2326 bits (147), Expect = 8.137e-10 Identity = 43/156 (27.56%), Postives = 73/156 (46.79%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160 H +CF C+ C++ + + +C+ F + C C ++I + T DQ +H E F C C L ++ +DD PFC+ C+ + + C C++ I+ +K + F++ WH +CF C C SLV F T + + C C Sbjct: 124 HETCFVCEHCRQPIGTKPL--ISKESGNYCVPCFEKEFAHYCNFCKKVITSGGIT-FRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q14192|FHL2_HUMAN (Four and a half LIM domains protein 2 OS=Homo sapiens OX=9606 GN=FHL2 PE=1 SV=3) HSP 1 Score: 295.049 bits (754), Expect = 3.283e-94 Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYILR+++P+CV CFE F NTC +C + I CD KDLS+K+ HWHE CF C C +SLVD+ F K++ L C +C+ ++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE +HA +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT++++ YC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.479 bits (306), Expect = 9.718e-30 Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y +S +C+ F L C EC + I KD + D+ +H F C C +SL + ++D C C+ N++ + C +C + I ++ + +K WHE CF+C C + +SF KDN +C C++++ + C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 4.288e-26 Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + +C EC + I+ T+ M+ +H F CH C + +I +D+ FCV C+E Q C +C + I+ + ++++E WH++CF+C C L + FT +D++ YC NCF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O70433|FHL2_MOUSE (Four and a half LIM domains protein 2 OS=Mus musculus OX=10090 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 290.812 bits (743), Expect = 1.162e-92 Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYIL+++NP CV CFE + NTC +C I CD KDLS+K+ HWHE CF C C SLVD+ F K+ L C +C+ ++SS C C K G +K EY G WHE CF C C Q IGTKSFIP + C+ CYE ++A +C +C + I G+TY+ PWHKECF+CT+C+K LS +FT+++E PYC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 119.013 bits (297), Expect = 1.486e-28 Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ C+ F EL C EC I KD + D+ +H F C C SSL + +++ C C+ N++ + C +C + I ++ + +K WHE CF C C + +SF K+N +C C++++++ C C K TG Y + WH++CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 103.99 bits (258), Expect = 2.127e-23 Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + + + + C + +C EC + I+ T+ M+ +H F C C + +I +++ FCV C+E Q+ C +C + I+ + ++++E WH++CF+C C L + FT +D + YC CF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O35115|FHL2_RAT (Four and a half LIM domains protein 2 OS=Rattus norvegicus OX=10116 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 290.426 bits (742), Expect = 1.922e-92 Identity = 136/285 (47.72%), Postives = 179/285 (62.81%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KYIL+++NP CV CFE + NTC +C I CD KDLS+K+ HWHE CF C C SLVD+ F K+ L C +C+ ++SS C C K G +K EY G WHE CF C C Q IGTKSFIP + C+ CYE ++A +C +C + I G+TY+ PWH+ECF+CT+C+K LS +FT+++E PYC CF +L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 119.013 bits (297), Expect = 1.337e-28 Identity = 76/276 (27.54%), Postives = 128/276 (46.38%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++ C+ F EL C EC I KD + D+ +H F C C SSL + +++ C C+ N++ + C +C + I ++ + +K WHE CF C C + +SF K+N +C C++++++ C C K TG Y + WH +CF+C C + + + F + C+ C+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 102.449 bits (254), Expect = 7.185e-23 Identity = 61/220 (27.73%), Postives = 102/220 (46.36%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C LVD + + + + C + +C EC + I+ T+ M+ +H F C C + +I +++ FCV C+E Q+ C +C + I+ + +++++ WH +CF+C C L + FT +D + YC CF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q2KI95|FHL2_BOVIN (Four and a half LIM domains protein 2 OS=Bos taurus OX=9913 GN=FHL2 PE=2 SV=1) HSP 1 Score: 287.73 bits (735), Expect = 2.206e-91 Identity = 135/285 (47.37%), Postives = 182/285 (63.86%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C+ SL G KY+LR++ P+CV CFE F +TC +C + I CD KDLS+K+ HWHE CF C C SLVD+ F K++ L C +C+ +++SS C C K G +K EY G WHE CFIC C Q IGTKSFIP + + C+ CYE ++A +C +C + I G+TY+ PWH+ECF+CT+C+K LS +FTS++E YC CF +L+ K+C C PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERFDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276 HSP 2 Score: 122.865 bits (307), Expect = 5.702e-30 Identity = 75/276 (27.17%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C++ L Y + +C+ F L C EC +LI KD + D+ +H F C C SL + ++D C C+ ++ + C +C + I ++ + +K WHE CF+C C + +SF KD+ +C C++ +++ C C K TG Y + WH +CF+C C + + + F + C+GC+ + +A+KC C+ I G I+++ WH +CF C C L F ++ + C +C Sbjct: 5 FDCHHCEDSLFGRKYVLREEQP--YCVACFEALFASTCEECGKLIGCDCKDLS-YKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTG--GVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 112.079 bits (279), Expect = 3.440e-26 Identity = 66/220 (30.00%), Postives = 105/220 (47.73%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H +CF C C+ LVD + D + C + + RC EC + I+ T+ M+ +H F CH C + +I +D FCV C+E Q+ C +C + I+ + ++++E WH +CF+C C L + FT++D + YC CF + ++ C GC G K + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEACFHCSRCRGSLVDKPFAAKEDQ--LLCTDCYSQEYSSRCQECKKSIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPIT--TGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q3ZBI6|FHL3_BOVIN (Four and a half LIM domains protein 3 OS=Bos taurus OX=9913 GN=FHL3 PE=2 SV=1) HSP 1 Score: 275.404 bits (703), Expect = 1.010e-86 Identity = 128/286 (44.76%), Postives = 178/286 (62.24%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748 E F C C SL G KYI DD P+CV C++N F NTC +C + I DS++L +++ H+HE CF C C SL D FT +D+ L C +C+ FSS C CG+ G +K EY G+ WHE CF+C+ C Q +G++SF+P G C+ CYENK A +C +CS+ + + G+TY+ PWH+EC +CT CQ L+ +FTS+E+ PYC CF ELF +C C +PI+ G GG K++S D HWH CF+C++C S+VG+GF DQ++C C A Sbjct: 3 EAFDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPIT----------GLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278 HSP 2 Score: 120.168 bits (300), Expect = 5.566e-29 Identity = 77/275 (28.00%), Postives = 110/275 (40.00%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C C E L Y ++D +C+ + CAEC +LI + D+ FH F C C SL + +D C C+ + F + C C E + S+ L + WHE CFLC C L RSF YC C++ KF+ C C K G Y + WH +C +C C + + F C+ C+ A KC C I G ++++ WH CF C C L F ++ C C Sbjct: 5 FDCAKCSESLYGRKYIQTDDGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275
BLAST of Four and A half lim domains vs. TrEMBL
Match: B1AAM6 (LIMPETin OS=Schistosoma mansoni OX=6183 PE=2 SV=1) HSP 1 Score: 491.886 bits (1265), Expect = 1.807e-164 Identity = 260/572 (45.45%), Postives = 350/572 (61.19%), Query Frame = 3 Query: 96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL-QDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIP--DCDRISS--EVQNHFDDKDED-KLIVQLARAFKKSRDTNYLGFGEIEPVDNQ--EVCSICDKQFEDEDIFCIRI--------------THSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C C+S C + H WR C C C Y H +D + S +D L L Q+Y A ++A+ L WLPIG++Q E+ FL P L + R+ + QIP DC+ + E+ NH D + + + ++ A FK R+++ LG G +E ++ + + C+ C D FCIRI T + A H++CF C TC E LVD+ Y W N K ++CLRH+G+ RC CD LI ++++T+AM+QE H HFACH CD SLTG +YILRDD P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C C SL DR F TK+ LYC +C+DE+F++ CDGC +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+ KFTSK+E+PYC +C+ ELF K+C KCT+PI+ GFGGCKFIS D HWH+ECF C KC +++VG+GF +D ++C CG Sbjct: 11 CLKCQSKCSGFMKHSWRMICTQCHCAYYEH--DIDYYYTNQSI--------LDELDYNQSILLYQEYMNAQNLAKQYGLNWLPIGVKQTEVDLFLNSL----PNEELPRGEVADYIRRQRLRKQIPLQDCNPAVTIEELWNHSKDSNPNLENELREANRFKNFRNSHDLGIGLVEHMNEKDGQPCTNCSNTIHF-DEFCIRIKPEHSLTEEISNVSTSNHTPAWHLNCFRCTTCNEYLVDYIYAWFN--KQLYCLRHYGQSIRPRCVTCDHLIFSEEYTRAMEQEHHTGHFACHSCDVSLTGQRYILRDDEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACTTSLADRPFATKEEQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFLCVECKQPIGAKSFIPRENQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCSKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEDRHWHSECFLCGKCNSNLVGRGFLTSDDMIMCSECG 555 HSP 2 Score: 113.235 bits (282), Expect = 1.599e-22 Identity = 71/232 (30.60%), Postives = 113/232 (48.71%), Query Frame = 3 Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 WH CF C C++ LVD + + LYC + + C C + + ++Y A +Q H F C C+ S+ + +I + + C+ CYE K A C +C E I +++K WH++CF C++C L++ F +KEE+ YC++C+ E F RC C Q + K +G WH ECF C +C+ + K F +E+Q+VC C A+ Sbjct: 219 WHLNCFRCTTCNEYLVDYIYAWFNKQLYCLRHYGQSIRPRCVTCDHLIFS--EEYTRAMEQEHHTGHFACHSCDVSLTGQRYILRDDEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACTTSLADRPFATKEEQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFLCVECKQPIGAKSFIPRENQVVCVPCYEAK 436
BLAST of Four and A half lim domains vs. TrEMBL
Match: U6J6Y3 (Four and a half LIM domains protein 2 OS=Echinococcus granulosus OX=6210 GN=EGR_09187 PE=4 SV=1) HSP 1 Score: 491.5 bits (1264), Expect = 2.053e-164 Identity = 254/569 (44.64%), Postives = 337/569 (59.23%), Query Frame = 3 Query: 96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITHSAEF----------ASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C C S C + H WR C +C C +H S DS ++ L + L+ Y A ++A + L WLP+G+Q +E+ FL P + R I +Q+P DR +S+ N L + A F+ SR+ G G +E + VC+ C +Q + FC+RI A H CF C C E LVDF Y W N +CLRH+G++ RCA CD LI ++++T+AMDQE H HFAC CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C C+ SL DR F TKD+ LYC +C+DE+F++ CDGC +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+ KFTSK+E+PYC +C+ +LF K+C KC +PI+ GFGGCKFIS D HWH+ECF CSKC ++VG+GF EDQ++C CG Sbjct: 11 CLTCASECPGFKLHPWRATCTACHCSYDKHAVTDPSKDS-----------YLHELELSDTTVLKAYDVAQTVAREHGLHWLPVGIQSSEVEAFLTSL----PSSEIPRGEAYVEMRTRRIRHQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCG 551 HSP 2 Score: 106.686 bits (265), Expect = 2.307e-20 Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3 Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 DS D S+ WH CF C C + LVD + + YC + + C C + + ++Y A Q H F C C+ S+ + +I + + C+ CYE K A C +C E I +++K WH++CF C++C L++ F +K+++ YC++C+ E F RC C Q + K +G WH ECF C +C+ + K F +++Q+VC C A+ Sbjct: 205 DSSDNSYVP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A068Y1Z9 (Four and A half lim domains OS=Echinococcus multilocularis OX=6211 PE=4 SV=1) HSP 1 Score: 491.5 bits (1264), Expect = 2.314e-164 Identity = 254/569 (44.64%), Postives = 337/569 (59.23%), Query Frame = 3 Query: 96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITHSAEF----------ASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C C S C + H WR C +C C +H S DS ++ L + L+ Y A ++A + L WLP+G+Q +E+ FL P + R I +Q+P DR +S+ N L + A F+ SR+ G G +E + VC+ C +Q + FC+RI A H CF C C E LVDF Y W N +CLRH+G++ RCA CD LI ++++T+AMDQE H HFAC CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C C+ SL DR F TKD+ LYC +C+DE+F++ CDGC +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+ KFTSK+E+PYC +C+ +LF K+C KC +PI+ GFGGCKFIS D HWH+ECF CSKC ++VG+GF EDQ++C CG Sbjct: 11 CLTCGSECPGFKLHPWRATCTACHCSYDKHAVTDPSKDS-----------YLHELELSDTTVLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLTSL----PSSEIPRGEAYVEMRTRRIRHQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCG 551 HSP 2 Score: 106.686 bits (265), Expect = 2.349e-20 Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3 Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 DS D S+ WH CF C C + LVD + + YC + + C C + + ++Y A Q H F C C+ S+ + +I + + C+ CYE K A C +C E I +++K WH++CF C++C L++ F +K+++ YC++C+ E F RC C Q + K +G WH ECF C +C+ + K F +++Q+VC C A+ Sbjct: 205 DSSDNSYVP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3W1E0 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS3546 PE=4 SV=1) HSP 1 Score: 489.96 bits (1260), Expect = 8.052e-164 Identity = 255/572 (44.58%), Postives = 341/572 (59.62%), Query Frame = 3 Query: 96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMN---IFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRIT----------HSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C C S C + H WR C +C C +H S DS ++ L + L+ Y A ++A + L WLP+G+Q +E+ FL IP + +M I +Q+P DR +S+ N L + A F+ SR+ G G +E + VC+ C +Q + FC+RI +S A H CF C C E LVDF Y W N +CLRH+G++ RCA CD LI ++++T+AMDQE H HFAC CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C C+ SL DR F TKD+ LYC +C+DE+F++ CDGC +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+ KFTSK+E+PYC +C+ +LF K+C KC +PI+ GFGGCKFIS D HWH+ECF CSKC ++VG+GF +DQ++C CG Sbjct: 11 CLTCGSECPGFKLHPWRATCTNCHCSYEKHAVTDPSKDS-----------YLHELEVSDTTLLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLT-------SLPSSEIPRGEAYAEMRFRRIRHQVPPQDRKPASSLSTAKLNLPPSNAPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYAPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCNCNLVGRGFLTNDDQIMCSDCG 551 HSP 2 Score: 107.071 bits (266), Expect = 1.748e-20 Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3 Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 DS D S+ WH CF C C + LVD + + YC + + C C + + ++Y A Q H F C C+ S+ + +I + + C+ CYE K A C +C E I +++K WH++CF C++C L++ F +K+++ YC++C+ E F RC C Q + K +G WH ECF C +C+ + K F +++Q+VC C A+ Sbjct: 205 DSSDNSYAP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3X1E4 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7003 PE=4 SV=1) HSP 1 Score: 488.034 bits (1255), Expect = 4.978e-163 Identity = 253/569 (44.46%), Postives = 338/569 (59.40%), Query Frame = 3 Query: 96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITH----------SAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C C S C + H WR C C C +H S DS ++ L + L+ Y A ++A+ + L WLP+G+Q AE+ FL P + + R I +Q+P DR +S+ N L + A F+ SR+ G G +E V + VC+ C + + FC+R+ S A H CF C C E LVD Y W N +CLRH+G++ RCA CD LI ++++T+AMDQE H HFAC CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C C+ SL DR F TKD+ LYC +C+DE+F++ CDGC +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KC+E+IR+ G+TYKG+PWHKECF CTSC K L+ KFTSK+E+PYC +C+ ELF K+C KC +PI+ GFGGCKFIS D HWH+ECF CSKC ++VG+GF +DQ++C CG Sbjct: 11 CLTCGSECSGFKLHPWRATCTGCHCSYDKHAVTDPSKDS-----------YLHELELSDVTVLKAYDVAQAIAKEHGLHWLPVGIQSAEVEAFLTSL----PSSEIPRGEVYAEMRFRRIRHQVPPQDRKPASSLSTAKLNLPPSTAPANLEGESREATRFQNSRNRRDFGIGRVERVSAKATIVCAECSEHIGFRE-FCVRVRPEHRLSDSSDTSYAPAWHPGCFRCSNCSEHLVDLVYGWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCAEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNDDQIMCSDCG 551 HSP 2 Score: 107.842 bits (268), Expect = 8.691e-21 Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3 Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751 DS D S+ WH CF C C + LVD + + YC + + C C + + ++Y A Q H F C C+ S+ + +I + + C+ CYE K A C +C E I +++K WH++CF C++C L++ F +K+++ YC++C+ E F RC C Q + K +G WH ECF C +C+ + K F +++Q+VC C A+ Sbjct: 205 DSSDTSYAP-AWHPGCFRCSNCSEHLVDLVYGWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:103031012]) HSP 1 Score: 285.034 bits (728), Expect = 2.524e-91 Identity = 134/285 (47.02%), Postives = 179/285 (62.81%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE+CF C C SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C C + I G+TY PWHK+CFLCT C++ LS +FTS+++ YC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276 HSP 2 Score: 135.961 bits (341), Expect = 1.561e-35 Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y ++ +C++ + L C EC + I N D+ +H E F C C SL + +D+ C +C+ N++ + CH+C + I S+ + K WHE CF C C + +SF KDN YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 105.145 bits (261), Expect = 1.073e-24 Identity = 67/220 (30.45%), Postives = 106/220 (48.18%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C CK LVD + S + + C + +C EC + I+ ++ M+ + +H F C C + +I +D+N +CV C+E QF C C + I+ + +++ + WH+ CFLC C L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEECFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:103023824]) HSP 1 Score: 274.633 bits (701), Expect = 2.689e-87 Identity = 129/284 (45.42%), Postives = 180/284 (63.38%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 E F C C SL G KYI +DNP+C+ C+++ F NTC +C E I D+++L +++ H+HE CF C C SL D FT++D+ L C +C+ +FSS C C K G +K EY G WHE CFIC C Q IG+KSFIP C+ CYENK A +C +C +++ K G+TY+ +PWHKECFLCT C+ L+ FTS+++ PYC +CF L+ K+C+ C++PI+ GFGG K+IS + WH CF CS+C S+VG GFF + D+++C C Sbjct: 2 TERFDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPIT----------GFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275 HSP 2 Score: 130.183 bits (326), Expect = 2.303e-33 Identity = 80/276 (28.99%), Postives = 131/276 (47.46%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C CKE L Y + D+ +C+ + L C EC ELI D + D+ +H F C CD SL + +DD C C+ N+F + C C + + ++ L + WHE CF+C+ C + +SF + YC C++ KF+ C C ++ G Y + WH++CF+C C + + F + C+ C+ N +A+KC CS+ I G I+++ WH+ CF C+ C L + F + ++ C +C Sbjct: 5 FDCDNCKESLYGRKYIQAEDNP--YCIPCYDSLFANTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKG--GVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275 HSP 3 Score: 121.709 bits (304), Expect = 1.671e-30 Identity = 66/224 (29.46%), Postives = 109/224 (48.66%), Query Frame = 3 Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 F CD C +SL R + ++ YC C+D F++ CD C ++ ++ Y + +HE CF C C++S+ + F + + C CY N+ + KC C + + + Y G WH+ CF+C SC++ + F ++ YC C+ F RC +C Q +L KG ++ D+ WH ECF C+ C+ + G+ F ++D C C G Sbjct: 5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQ---------VLAKGG-----VTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFG 214 HSP 4 Score: 102.064 bits (253), Expect = 1.273e-23 Identity = 62/223 (27.80%), Postives = 107/223 (47.98%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352 H CF C C L D + +D A+ C + +C CD+ ++ T+ ++ +H F C+ C+ + +I D+ +CV C+EN+F C +C + ++ +++++ WH++CFLC C L + FT++D+ YC CF ++ C+ C K G K + +QWH+ CF C+ C+ S+ F P +I C C N Sbjct: 62 HEHCFRCFRCDRSLADEPFTSQDD--ALLCNDCYCNEFSSKCVACDKTVMPG--TRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLA--KGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSN 278
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 232.261 bits (591), Expect = 1.909e-71 Identity = 120/285 (42.11%), Postives = 166/285 (58.25%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C C+ SLVD+ F+TK+ L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ C+E + A +C +C ++ T +CFLCT C++ LS +FTS+++ PYC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC D ++C CG Sbjct: 2 AERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNST--------DCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSI-----------DDILCPDCG 257 HSP 2 Score: 108.227 bits (269), Expect = 6.419e-26 Identity = 70/254 (27.56%), Postives = 118/254 (46.46%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV-NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSC 1457 ++C CKE L Y ++ +C++ + L C EC + I N D+ +H + F C C+ SL + +++ C +C+ N++ + C++C + I S+ + K WHE CF C C + +SF KDN YC CF+++F+ C C K+ DCF+C C + + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C Sbjct: 5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNS----------TDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKC 246 HSP 3 Score: 79.7221 bits (195), Expect = 3.595e-16 Identity = 58/200 (29.00%), Postives = 90/200 (45.00%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCN 1283 H CF C C LVD + S ++ + C + +C EC + I+ ++ M+ + +H F C C + +I +D+ +CV CFE QF C +C + + CFLC C + L + FT++D++ YC NCF ++ C C I G KY + +QWH DCF C C+ Sbjct: 62 HDDCFHCFKCNRSLVDKPF--STKNEELLCTECYSNEYSSKCYECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKK----------VHTVYNSTDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCS 247
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707]) HSP 1 Score: 210.69 bits (535), Expect = 5.988e-63 Identity = 111/287 (38.68%), Postives = 154/287 (53.66%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDN-YLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F C C +L G KY+ +DD CV+CFE NTC +C I D+K+L+ K HWHE CF C C+ L + SF +D+ + C C + S+ C GC K+ G K EY K WHE+CF C +C Q I + SF+ C C+E K A+ C +C E I +GITY+ PWH ECF+C +C+K L+ +FT+ E+ YC +C+ K+C C PI+ GFG G ++ ++ WH CFNC KC S+ K F L + + C C Sbjct: 2 TERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPIT----------GFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCA 278 HSP 2 Score: 120.553 bits (301), Expect = 4.970e-30 Identity = 85/282 (30.14%), Postives = 125/282 (44.33%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNP-FCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C C++ L Y +D C+R F +L CAEC I V+ ++ +H + F C C L + RDD C KC + C C + I K++ +K WHE+CF C C + SF TK + YC C ++KF+ C C + I G+ Y K WH +CF+C C + + F C+ CY+ A+KC C I G + Y+ + WH+ CF C C L+ +F E YCT+C +L Sbjct: 5 FDCFYCRDNLQGKKYVNKDDKHV--CVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGI---TYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 281 HSP 3 Score: 101.293 bits (251), Expect = 2.362e-23 Identity = 60/219 (27.40%), Postives = 96/219 (43.84%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE-FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKI---FRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C + L + + +D K + C + RC C ++I+ D + +H E F C C + ++ + D+ +C C E +F C +C E I+ + +++++ WH +CF+C C L FT ++Y YC +C+ + C GC F G Y WHE CF C C+ S+ K F+ I C C Sbjct: 62 HEDCFRCAKCYKPLANESFAARDDGK-VMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPIT--ANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 277 HSP 4 Score: 54.299 bits (129), Expect = 1.378e-7 Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 3 Query: 1434 ECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQED-QMVCGSCGGARNDDG 1763 E F C C+ L K+ +K++K C CF +L C +C +PI K ++ + HWH +CF C+KC + + F ++D +++CG C GAR D Sbjct: 3 ERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDA------------KELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKC-GAREDSA 100
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707]) HSP 1 Score: 211.075 bits (536), Expect = 6.303e-63 Identity = 111/287 (38.68%), Postives = 154/287 (53.66%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDN-YLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F C C +L G KY+ +DD CV+CFE NTC +C I D+K+L+ K HWHE CF C C+ L + SF +D+ + C C + S+ C GC K+ G K EY K WHE+CF C +C Q I + SF+ C C+E K A+ C +C E I +GITY+ PWH ECF+C +C+K L+ +FT+ E+ YC +C+ K+C C PI+ GFG G ++ ++ WH CFNC KC S+ K F L + + C C Sbjct: 18 TERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPIT----------GFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCA 294 HSP 2 Score: 120.939 bits (302), Expect = 5.180e-30 Identity = 85/282 (30.14%), Postives = 125/282 (44.33%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNP-FCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532 F+C C++ L Y +D C+R F +L CAEC I V+ ++ +H + F C C L + RDD C KC + C C + I K++ +K WHE+CF C C + SF TK + YC C ++KF+ C C + I G+ Y K WH +CF+C C + + F C+ CY+ A+KC C I G + Y+ + WH+ CF C C L+ +F E YCT+C +L Sbjct: 21 FDCFYCRDNLQGKKYVNKDDKHV--CVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGI---TYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 297 HSP 3 Score: 101.679 bits (252), Expect = 2.333e-23 Identity = 60/219 (27.40%), Postives = 96/219 (43.84%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE-FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKI---FRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF C C + L + + +D K + C + RC C ++I+ D + +H E F C C + ++ + D+ +C C E +F C +C E I+ + +++++ WH +CF+C C L FT ++Y YC +C+ + C GC F G Y WHE CF C C+ S+ K F+ I C C Sbjct: 78 HEDCFRCAKCYKPLANESFAARDDGK-VMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPIT--ANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 293 HSP 4 Score: 54.6842 bits (130), Expect = 1.219e-7 Identity = 37/127 (29.13%), Postives = 57/127 (44.88%), Query Frame = 3 Query: 1386 IIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQED-QMVCGSCGGARNDDG 1763 R SG E F C C+ L K+ +K++K C CF +L C +C +PI K ++ + HWH +CF C+KC + + F ++D +++CG C GAR D Sbjct: 3 FYRHSGPRSYLSSTMTERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDA------------KELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKC-GAREDSA 116
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 166.777 bits (421), Expect = 1.696e-48 Identity = 77/172 (44.77%), Postives = 105/172 (61.05%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR-TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG 1403 E + CH C SL G KY+LR++NP+CV+C+E + + C C + I CDSKDLS+K+ HWHE CF C C SL D++F+ +D L C C +S+ C G K+F G +K E+ G WHE CF CA C Q IG+KSF+P C+ CYE + AQ C C ++ + G Sbjct: 11 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGG 182 HSP 2 Score: 97.4413 bits (241), Expect = 1.221e-23 Identity = 46/164 (28.05%), Postives = 82/164 (50.00%), Query Frame = 3 Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG---ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQ 1562 + C C +SL+ + + ++ YC C++ ++ C+ C + K Y + WHE CF CA C +S+ K+F + K+ C C+ N ++ KC S++ G + ++G WH+ CF C CQ+ + F +E+ YC C+ + F + C C + Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK 176 HSP 3 Score: 76.6406 bits (187), Expect = 1.741e-16 Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 3 Query: 1263 FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 + C C +S+ K ++ C+ CYE + C C + I ++YK WH+ CF C +C++ L++ F++++EK CTEC + +C ++ S K+ +G WH CF C++CQ + K F +E C C Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRG-----------SSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 162 HSP 4 Score: 73.1738 bits (178), Expect = 2.934e-15 Identity = 47/172 (27.33%), Postives = 80/172 (46.51%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKIS-CDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTG 1217 ++C CKE L+ Y ++ +C+R + L C C + I +KD + D+ +H F C C+ SL + RD+ C +C N + C S+ S ++ + F+ WHE CF C C + +SF ++ YC C++++F+ C C K+ G Sbjct: 13 YDCHYCKESLLGKKYVLREENP--YCVRCYETLYTHACESCKQPIGCDSKDLS-YKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 181 HSP 5 Score: 62.3882 bits (150), Expect = 1.818e-11 Identity = 34/104 (32.69%), Postives = 52/104 (50.00%), Query Frame = 3 Query: 1434 ECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGC--KFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 E + C C++ L K+ +EE PYC C+ L+ C+ C QPI GC K +S D HWH CF C+ C+ S+ K F ++++++C C Sbjct: 11 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPI--------------GCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTEC 100
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 161.77 bits (408), Expect = 1.531e-46 Identity = 75/173 (43.35%), Postives = 102/173 (58.96%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGIT 1409 E + CH C SL G KY+LR++NP+CV+C+E + + C C + I CDSKDLS+K+ HWHE CF C C SL D++F+ +D L C C +S+ C G +K E+ G WHE CF CA C Q IG+KSF+P C+ CYE + AQ C C ++ + G T Sbjct: 4 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCA--ANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPT 174 HSP 2 Score: 97.8265 bits (242), Expect = 9.017e-24 Identity = 46/174 (26.44%), Postives = 82/174 (47.13%), Query Frame = 3 Query: 1086 CDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGF 1607 C C +SL+ + + ++ YC C++ ++ C+ C + K Y + WHE CF CA C +S+ K+F + K+ C C+ N ++ KC + + ++G WH+ CF C CQ+ + F +E+ YC C+ + F + C C + + +L F Sbjct: 8 CHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPTRLSPAPF 181 HSP 3 Score: 75.8702 bits (185), Expect = 4.024e-16 Identity = 42/161 (26.09%), Postives = 71/161 (44.10%), Query Frame = 3 Query: 1263 FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 + C C +S+ K ++ C+ CYE + C C + I ++YK WH+ CF C +C++ L++ F++++EK CTEC + +C P+ G + + WH CF C++CQ + K F +E C C Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPV--------------GTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 152 HSP 4 Score: 72.7886 bits (177), Expect = 5.414e-15 Identity = 45/171 (26.32%), Postives = 78/171 (45.61%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTG 1217 ++C CKE L+ Y ++ +C+R + L C C + I +KD + D+ +H F C C+ SL + RD+ C +C N + C + ++ + F+ WHE CF C C + +SF ++ YC C++++F+ C C K+ G Sbjct: 6 YDCHYCKESLLGKKYVLREENP--YCVRCYETLYTHACESCKQPIGCDSKDLS-YKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVG--TRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 171
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl3b (four and a half LIM domains 3b [Source:ZFIN;Acc:ZDB-GENE-030131-8356]) HSP 1 Score: 161.384 bits (407), Expect = 6.549e-46 Identity = 83/237 (35.02%), Postives = 116/237 (48.95%), Query Frame = 3 Query: 1044 LSFKEWHWHEKCFLCDLCHDSL-VDRSFTTKDNYLYCPNCFDEK---FSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 LS HWHE CF C C + + + + C C C C + R GV K Y G+ WHE CF CA C ++IGTKSF+P +I C+ C E K A +CF C + + + G+ YK +H+EC +C C L+ ++ E YC CF + + K+C C +PI+ G KF++ WHTECF C +C +M GKGF + D + CG C Sbjct: 2 LSLDNLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSA----------AGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCA 228 HSP 2 Score: 90.5077 bits (223), Expect = 7.612e-21 Identity = 56/221 (25.34%), Postives = 100/221 (45.25%), Query Frame = 3 Query: 882 FHVEHFACHVCDSSL-TGTKYILRDDNPFCVKCFENQ---FGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 +H F C C + + T+Y+L C C + G++C C I +++K WHE+CF C C +++ +SF + +YC +C ++K ++ C C K K Y +H +C +C CN + + + C C+E K+++KC C + I + +T+ WH ECF C C ++ F ++ + +C +C Sbjct: 9 WHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTE--KGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDC 227 HSP 3 Score: 63.929 bits (154), Expect = 1.076e-11 Identity = 39/159 (24.53%), Postives = 69/159 (43.40%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKI--SCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDE 1169 H CF C C E + + D I+C+ + T RC C + + K + +H E C C++ L G R + +C CFE ++ C C + I + +K L+ WH +CF C C ++ + F + + ++C +C ++ Sbjct: 75 HERCFACARCMETIGTKSFVPHGDE--IYCVSCNEKKTATRCFTCHKPVTEKGVVYK-EHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQ 230
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718]) HSP 1 Score: 109.383 bits (272), Expect = 1.872e-27 Identity = 64/226 (28.32%), Postives = 101/226 (44.69%), Query Frame = 3 Query: 861 TKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535 AM + +H EHF C C + + +D P+C + + + F C C+E + + + ++ + WH + F C C + F +D YC + F+ C+GC R ++ Y A WH +CFIC +C SF G+ C Y + C C + I I+ +H E F+C C K L++ F + +KPYC CFI+LF Sbjct: 3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCS---RAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLF 225 HSP 2 Score: 90.8929 bits (224), Expect = 6.046e-21 Identity = 64/225 (28.44%), Postives = 89/225 (39.56%), Query Frame = 3 Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 WH + F+C C + R+F KD YC + FS C C + + + WH + F CA C + G + F +GK C Y A KC CS I ++ ++ WH ECF+C C F E +PYC + E C C +PI+ G C IS +H E F C+ C + F Q D+ C C Sbjct: 10 WHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPIT------------GRC--ISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPC 220 HSP 3 Score: 83.9593 bits (206), Expect = 1.358e-18 Identity = 56/189 (29.63%), Postives = 86/189 (45.50%), Query Frame = 3 Query: 783 FCLRHFGELTYCRCAECDELIV-NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCY 1346 +C + L RCA C+E ++ N+ A+D+ +H EHF C C + RD P+C + + F C+ CS I LS WH +CF+C C ++ SF + YC + E+ S C GC K TG + K++H + F+CA C + + +F N K C C+ Sbjct: 37 YCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIM--QNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPI-TG-RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCF 221 HSP 4 Score: 58.9214 bits (141), Expect = 5.054e-10 Identity = 31/109 (28.44%), Postives = 49/109 (44.95%), Query Frame = 3 Query: 1404 ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730 +T G WH E F+C CQ + F K+ +PYC E + LF RC C + + D + + ++ D WH E F C++C +GF ++ + C Sbjct: 3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQ------------RVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYC 99 HSP 5 Score: 46.9802 bits (110), Expect = 4.014e-6 Identity = 41/192 (21.35%), Postives = 77/192 (40.10%), Query Frame = 3 Query: 603 VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFS 1178 +DNQ V + D+ + E FC + F FH D K +C + + + +C C I+ +++ A++ +H E F C C + + + P+C + + G+ C C + I+ + +S +H + F+C C L +F +++ YC CF + F Sbjct: 58 MDNQRVVTALDRTWHPEHFFCAQC--GRPFGEE--------------GFH---ERDGKP-YCRQDYYGMFAPKCNGCSRAIM-QNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPIT--GRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLFG 226
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718]) HSP 1 Score: 114.39 bits (285), Expect = 4.691e-27 Identity = 77/244 (31.56%), Postives = 107/244 (43.85%), Query Frame = 3 Query: 807 LTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535 L +CA C + I + T AM + +H EHF C C + + RD P+C K + F C CS I K ++ + WH + F C C D F KD YC + E F+ C GC + V+ Y A WH +CF+C +C SF G+ C Y + C C + I IT +H E F+C C K L++ F + +KPYC CFI+LF Sbjct: 317 LAKGQCAACLKPIAGQVVT-AMGRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPIL--DKVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAI---VENYISALSALWHPECFVCRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFIKLF 554 HSP 2 Score: 89.3521 bits (220), Expect = 5.082e-19 Identity = 48/164 (29.27%), Postives = 71/164 (43.29%), Query Frame = 3 Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730 G+ WH + F+C +C + IG ++F +G C Y + +C CS I +T WH E F C C + F K+ KPYC + + E+F +C C++ I + +IS WH ECF C +C + FF E Q C Sbjct: 338 GRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILDKVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAIVEN--------------YISALSALWHPECFVCRECFTPFINGSFFEHEGQPYC 487 HSP 3 Score: 82.8037 bits (203), Expect = 6.178e-17 Identity = 64/226 (28.32%), Postives = 88/226 (38.94%), Query Frame = 3 Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 WH + F+C C + + R+F +D YC + FS C C G I V + K WH + F C C G + F +GK C Y A KC CS I ++ I+ WH ECF+C C F E +PYC + E C C +PI+ + K +H E F C+ C + F Q D+ C C Sbjct: 341 WHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILDKVVTALD---KTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAIVENYISALSALWHPECFVCRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKK--------------FHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 549 HSP 4 Score: 62.003 bits (149), Expect = 2.306e-10 Identity = 36/122 (29.51%), Postives = 54/122 (44.26%), Query Frame = 3 Query: 1365 KCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730 +C C + I +T G WH E F+CT CQ+ + F ++ PYC + + LF RC C+ PI D K ++ D WH E F C +C +GF ++ + C Sbjct: 321 QCAACLKPIAGQVVTAMGRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILD--------------KVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYC 428 HSP 5 Score: 48.521 bits (114), Expect = 4.208e-6 Identity = 29/99 (29.29%), Postives = 48/99 (48.48%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFG 995 H CF C+ C ++ + + ++ + +C H+ E C+ C + I + T AM ++FH EHF C C L + ++D P+C CF FG Sbjct: 460 HPECFVCRECFTPFINGSF-FEHEGQP-YCEVHYHERRGSLCSGCQKPITGRCIT-AMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFIKLFG 555
BLAST of Four and A half lim domains vs. Ensembl Yeast
Match: LRG1 (GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains [Source:SGD;Acc:S000002399]) HSP 1 Score: 49.2914 bits (116), Expect = 1.894e-6 Identity = 47/151 (31.13%), Postives = 60/151 (39.74%), Query Frame = 3 Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKIS-----CIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDH-WHTECFNCSK 1673 GK +HE CF C DC + + K F K S C Y +H C C +R T G + +E F CT C K + YC F++ F KRCK C PISD +F + H WH EC+ K Sbjct: 50 GKYYHESCFTCQDCQKPLKPKYFPYQVDKTSESILLCQYDYFRRHNLLCHVCDTPLRGLYYTAFGYRYDEEHFSCTICATPCGVKKCFMYGNQLYCKYHFLKYFSKRCKGCEFPISDQYI-----------EFPKGEEIHCWHPECYGIHK 189
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41100 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D2]) HSP 1 Score: 290.426 bits (742), Expect = 1.310e-90 Identity = 169/547 (30.90%), Postives = 277/547 (50.64%), Query Frame = 3 Query: 114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGP-GFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIE-PVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKE-WHWHEKCFLCDLCHDSL-VDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C ++ H W+ C C+CP H Q+ N +D T + L IS L W P GL Q+++ +++ + P +++ P ++ R + YQ+P DR E + F + + F S D N G G ++ P ++ C+ C + + AE + H CF C+TC E LV+ Y D ++C RHF EL RC CDELI ++T AM++ +H+ HF C CD S+TG ++I+R D P C CF++ + + C C +KI +S+D+S + HWH+KCF+CD+C L + S + + C C+ + C CG+I +G + EY+G WHE+CF CA+C ++IGT F+P + C GCY++K +++C C E + + G+ Y G+ WHK CF C C + L+ + F+ ++ YC EC+ + + K+C+ C +K G ++ ++ + ++H ECF CS+C S+ +GF + D+++CG C Sbjct: 20 CPGFSLHVWKKACQYCKCPREDH------------DIQNEANETMDINTRINGIDLPPPPKVISGGFPKYL-WTPSGLSQSQVELYMK---ELPPQKVPIKDSPGSKYYR-AQMAYQLPVQDR-DPEKLSTFSAAHRESFM-----QFNSSAD-NARGEGVVKVPFGRRQRCARCSHPILRSEPAVVAENIGAEASFHPGCFTCETCNELLVELTYFQHADK--VYCGRHFAELQKSRCGGCDELIFTGEYTVAMNKNWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQKIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEIC-------------LKAIVGGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCA 527 HSP 2 Score: 117.857 bits (294), Expect = 5.406e-28 Identity = 81/320 (25.31%), Postives = 134/320 (41.88%), Query Frame = 3 Query: 819 RCAECDELIVNKDFTK-----AMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAG---KQWHEDCFICADCNQSIGTK-SFIPINGKISCIGCYENKHAQKCFKCSEII--RKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 RCA C I+ + + FH F C C+ L Y D +C + F + C C E I ++ + +WH F C C S+ R F + + C +CF + ++ C+ C + + G + + + + WH+ CFIC C + + ++ S K C CY + ++C C +II S + Y G+ WH+ CF C +C + + S F K++ +C C+ F KRC C +P+ + L + WH CF+C C S+ F +++ C C G Sbjct: 166 RCARCSHPILRSEPAVVAENIGAEASFHPGCFTCETCNELLVELTYFQHADKVYCGRHFAELQKSRCGGCDELIFTGEYTVAMNK-NWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQ--KIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVL--------------YNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYG 468 HSP 3 Score: 63.929 bits (154), Expect = 9.259e-11 Identity = 43/166 (25.90%), Postives = 76/166 (45.78%), Query Frame = 3 Query: 669 RITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFD 1166 R+ +S F H +CF C C E + + +D+ FC + RCA C E ++ + +H F+C+ C SL + +RD +C++C+ + C C + I + + +E ++H++CF+C C SL F + + L C +C D Sbjct: 368 RLEYSGNFW-HENCFRCANCGEAIGTSGFVPKDDT--FFCPGCYQSKFSKRCASCGEPLLEGGVLYN-GETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAIV-GGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCAD 528
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: LIMPET (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX6]) HSP 1 Score: 273.863 bits (699), Expect = 4.655e-84 Identity = 169/550 (30.73%), Postives = 253/550 (46.00%), Query Frame = 3 Query: 114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIP----INQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARA---FKKSRDTNYLGFGEIEPVDNQE-VCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 C ++ H WR C SC+CP+ HV ++ D + + V + SH + A W+P GL EI ++ + + + IP + R + QIP D F K KL + A F + R LG G + Q C C+ D+ + H+ CF C+ E LVD YC +D I+C RH+GE RCA C+ELI ++++A+++ +H H C CD SL+ K++ + +P C +C++ F N C C E I SKD+ + HWHE CF C C L++ FT KD L C C S C C F G KK Y K +H+ CFIC +C Q IG+K FI + + C C+++K A+ C KC+++I+ S + + G +H ECF C C K L+ S FT +E + C C+ E + KRC C I G KF++ + ++H ECF C KC + G+ F +++ + +C C Sbjct: 41 CRGFSVHPWRKFCNSCKCPHELHVLSTNNNDQNTPEERMGVEEEDPPMLQGSH---------LQQAMMEGYEWVPRGLTSEEIQIYMSLLPE-------DKIPKLKSLGAQHRTKQLSLQIPLQD---------FSSKHCKKLTLDQKMAMDDFCEKRIKTALGVGAVRACMAQPSTCKRCNGPVNPGDMALFAERLGPDSCWHVKCFTCEEDGELLVDLIYCSKDDE--IYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGPGSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRGINPSKVCAACNGDFAPGEKKVGYQSKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSSVQHAGSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIE------------GNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPC 551 HSP 2 Score: 81.6481 bits (200), Expect = 2.859e-16 Identity = 48/167 (28.74%), Postives = 76/167 (45.51%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 WH CF C + + + + + +I C + K +C C E+I + + WH C+ C + LS KF + E P C C+ E F RC+ C +PI G K + V HWH CF CS+C ++ +GF L++++++C C G Sbjct: 218 WHVKCFTCEEDGELLVDLIYCSKDDEIYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGP------------GSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRG 372 HSP 3 Score: 65.0846 bits (157), Expect = 5.258e-11 Identity = 43/189 (22.75%), Postives = 83/189 (43.92%), Query Frame = 3 Query: 603 VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDE 1169 ++ +VC+ C+ F + ++ + ++ H CF C CK+ + + ++ + C F C +C+++I A +H E F CH CD L G+ + ++ C C+ ++ C C I ++K +++ E ++H +CF C C+ L F +D C C D+ Sbjct: 373 INPSKVCAACNGDFAPGEK---KVGYQSK-TFHDKCFICDECKQPIGSKQFIRRDERR--LCNNCFDSKFAKVCVKCNQVIKTSSVQHA-GSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIEGNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPCDDD 554
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49798 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX7]) HSP 1 Score: 142.51 bits (358), Expect = 1.990e-37 Identity = 106/365 (29.04%), Postives = 162/365 (44.38%), Query Frame = 3 Query: 111 SCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIP----INQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFAS--------HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWH--EKCFLCDLCHDSLVDRSFTTKDNYLYC-PNC 1160 +C ++ H WR C +C+CP H D +G N + + + D + ++ E W+P GL ++ ++ + E +P I + R I Q+P D + N ++++ +L F R + LG G ++ V C + E C+ A +AS H SCF C TCKE LVD Y + DSK ++C RH E RCA CDE+I + T+A D +HV+HF C CD L G +Y++RD+ P+C CFE+ + C C E I D+ ++ HWH +CF C C +L+ F K +YC P+C Sbjct: 19 TCPGFSAHYWRKICRNCKCPREDHNIISDDARAG--------RNIYMADVHRASSLASDDDSGCALDE---YAWVPPGLGPEQVHSYMNALPE-------EKVPYVDSIGERYRNRQIILQLPPHDN-EARFCNGLSEEEKRELCF-----FVALRKRDSLGRGVVKQVPEMSEGYSCKECGE-----CVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIY-FYKDSK-VYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCSPDC 352 HSP 2 Score: 73.1738 bits (178), Expect = 5.991e-14 Identity = 56/186 (30.11%), Postives = 83/186 (44.62%), Query Frame = 3 Query: 981 ENQFGNTCHKCSEKISCDSKDL--SFKEWH--WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRK--SGITYKGDPWHK--ECFLCTSCQKMLSESKFTSKEEKPYCT 1514 E G +C +C E ++ S + S H WH CF+C C + LVD + KD+ +YC E C C +I + E WH F C +C+ +G ++ + K C C+E+ +A+ C C E I S + + G WH ECF C +C K L F K + YC+ Sbjct: 165 EMSEGYSCKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEII-FAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349 HSP 3 Score: 72.0182 bits (175), Expect = 1.664e-13 Identity = 51/189 (26.98%), Postives = 78/189 (41.27%), Query Frame = 3 Query: 1185 CDGCGKIFRTG-VKKYEYAGKQ---WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHT--ECFNCSKCQASMVGKGFFLQEDQMVC 1730 C CG+ G + Y Q WH CFIC C + + + + K+ C + +C C EII T D WH + F C C L ++ ++ KPYC CF L+ + C C +PI + ++ G HWH ECF+C C +++G F + ++ C Sbjct: 172 CKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPI-EPDASQMAHNG-----------QHWHATNECFSCCTCGKALLGLPFLPKSGEIYC 348
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S548]) HSP 1 Score: 123.635 bits (309), Expect = 9.084e-32 Identity = 75/241 (31.12%), Postives = 116/241 (48.13%), Query Frame = 3 Query: 822 CAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKI--SCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535 CA C++ I+ + T A+ + +H EHFAC C++ L + RD P+C + + + F C C+ I SC ++ + WH + F+C C + D F +D +C + F+ C GCG+ + Y A WH +CFIC +C Q SF +G+ C Y K C+ C + I IT +H E F+C C K L++ F + +KPYC CF++LF Sbjct: 26 CAACNKPIIGQVCT-ALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSC----VTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI---MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITGRCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLF 258 HSP 2 Score: 101.293 bits (251), Expect = 5.490e-24 Identity = 53/164 (32.32%), Postives = 76/164 (46.34%), Query Frame = 3 Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730 GK WH + F C C +GT++F +GK C Y + A +C C+ I S +T WH E F+C C ++ F ++ KP+C E + +F RC C QPI D +IS HWH ECF C++C+ G FF + + C Sbjct: 42 GKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDN--------------YISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYC 191 HSP 3 Score: 84.7297 bits (208), Expect = 2.376e-18 Identity = 62/226 (27.43%), Postives = 93/226 (41.15%), Query Frame = 3 Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 WH + F C C L ++F +D YC + + F+ C C G I + V + + WH + F+CA+C G F +GK C Y A +C C + I + I+ WH ECF+CT C++ F + +PYC + C C +PI+ G C I+ +H E F C+ C + F Q D+ C C Sbjct: 45 WHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALD---QTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPIT------------GRC--ITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 253 HSP 4 Score: 48.1358 bits (113), Expect = 4.393e-6 Identity = 29/99 (29.29%), Postives = 45/99 (45.45%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFG 995 H CF C C++ + + +D + +C H+ C C + I + T AM ++FH EHF C C L + ++D P+C CF FG Sbjct: 164 HAECFICTECRQPFPGGSF-FDHDGRP-YCEMHYHAKRGTLCYSCQKPITGRCIT-AMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49801 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY0]) HSP 1 Score: 122.479 bits (306), Expect = 3.254e-30 Identity = 92/362 (25.41%), Postives = 154/362 (42.54%), Query Frame = 3 Query: 114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESN-------NLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLD----RKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEP---VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWH--EKCFLCDLCHDSLVDRSFTTKDNYLYC 1151 C+ H WR C +C+C H + D + +H V + + + +H L+DY ++ A W+P G+ Q + ++ E +PI + R+ + YQ+P D + N D + K+ F R+ + LG G+I ++ C C K ++ + H CF C C LVD Y + + I+C RH+ E RCA CDELI ++ +T+A D+ +H HF C CD L G Y+ R P C++C++ + C C + I+ D+K + + WH +CF C C+ ++ + F N ++C Sbjct: 38 CEGLDLHFWRKICKNCKCKQEDHDLYEDIAN------EHEVKSRLFKDSNVAH--LRDYFNKLTAANDQPKTEFVKKFTWVPPGMNQGILVKYME-------SLPKELVPIVGSEGAKYRRAQMVYQLPIHDHDPNYCDN-LSDAERQKM-----EDFCAMRNQDALGVGDIREKTQAASKWNCFRCSKPVMTGEVAVFASRAGEDKCWHPGCFVCTVCNNLLVDLIYFYKDG--VIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFC 376 HSP 2 Score: 83.5741 bits (205), Expect = 3.256e-17 Identity = 46/155 (29.68%), Postives = 78/155 (50.32%), Query Frame = 3 Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHK--ECFLCTSCQKMLSESKFTSKEEKPYCT 1514 WH CF+C +C++ LVD + KD +YC + E+F C C + IF + E + WH+ F C +C++ +G + ++ G+ C+ CY+ +A+ C C + I I ++G WH ECF C C K + +F + +C+ Sbjct: 225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAE--DRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCS 377 HSP 3 Score: 77.0258 bits (188), Expect = 4.273e-15 Identity = 46/164 (28.05%), Postives = 72/164 (43.90%), Query Frame = 3 Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWH--TECFNCSKCQASMVGKGFFLQEDQMVC 1730 WH CF+C CN + + +G I C Y + +C C E+I T D WH+ F C C + L + ++ +P+C EC+ + + K C C + I+ K I WH +ECF+C+KC M+GK F ++ + C Sbjct: 225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIA------------ADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFC 376
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:101169645]) HSP 1 Score: 286.189 bits (731), Expect = 7.024e-92 Identity = 135/285 (47.37%), Postives = 180/285 (63.16%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E + CH C SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+++DLS+K+ HWHE CF C C SLVD+ F+TKD L C C+ ++SS C C K G +K E+ G WHE CF C C Q IGTKSFIP + C+ CYE + A +C C + I G+TY+ PWHK+CFLCTSC++ LS +FTSK++ YC CF L+ K+C CT PIS G GG K+IS + WH +CFNC KC S+VG+GF + D ++C CG Sbjct: 2 TERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276 HSP 2 Score: 137.117 bits (344), Expect = 7.266e-36 Identity = 82/275 (29.82%), Postives = 132/275 (48.00%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 ++C CKE L Y ++ +C++ + L C EC + I N D+ +H + F C C SL + +D+ C +C+ N++ + CH+C + I S+ + K WHE CF C C + +SF KDN+ YC C++++F+ C C K TG Y + WH+DCF+C C Q + + F + C+ C+ N +A+KC C+ I G I+++ WH +CF C C L F ++ + C EC Sbjct: 5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 107.457 bits (267), Expect = 1.584e-25 Identity = 68/220 (30.91%), Postives = 108/220 (49.09%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH---FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CF+C CK LVD + S + + C + +C EC + I+ ++ M+ + H H F C C + +I +D++ +CV C+E QF C C + I+ + +++++ WH+ CFLC C L + FT+KD++ YC NCF ++ C C I G KY + +QWH DCF C C+ S+ + F+ I C C Sbjct: 62 HEDCFKCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl5 (four and a half LIM domains protein 2-like [Source:NCBI gene;Acc:101156370]) HSP 1 Score: 278.87 bits (712), Expect = 6.656e-89 Identity = 130/284 (45.77%), Postives = 174/284 (61.27%), Query Frame = 3 Query: 891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 E F CH C SL G KYI+++D +C KC+EN F N C +CS I C+SKDLS+K+ HWHE+CF C C SL D++F +++ L C C+ +SS C C K G +K EY G WHE CF+C C Q +GTKSFIP C+ C+E + A +C C + I G+TY+ PWH+ECFLC C++ LS +FTS+E PYC ECF L+ K+C CT+PI+ G K+IS + WH+ECFNC +C S+VG+GF Q D ++C CG Sbjct: 5 ERFDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITS----------LAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECG 278 HSP 2 Score: 134.42 bits (337), Expect = 6.982e-35 Identity = 84/278 (30.22%), Postives = 130/278 (46.76%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIE 1529 F+C CK+ L+ Y D++ +C + + L C EC I N D+ +H + F C C SL + R+D C +C+ + + C C + I S+ + +K WHE CFLC C L +SF K+N +C CF+++F+ C C K TG Y+ K WH +CF+C C + + + F C+ C+ N +A+KC C++ I I+++ WH ECF C C L F ++ + CTEC E Sbjct: 7 FDCHYCKDSLLGKKYIMKEDTQ--YCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQ--DKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGRE 280 HSP 3 Score: 107.071 bits (266), Expect = 2.609e-25 Identity = 66/224 (29.46%), Postives = 104/224 (46.43%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355 H CF+C C L D + D + C + +C C + I+ ++ M+ +H F CH C L +I +++ FCV CFE QF C C + I+ + +++++ WH +CFLC C L + FT+++NY YC CF ++ C GC K + G K + +QWH +CF C C+ S+ + F+ I C C K Sbjct: 64 HEQCFKCAKCSRSLADKAFAARED--LLLCTECYALDYSSKCITCKKTILPG--SRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAIT--TGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGREK 281
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 277.715 bits (709), Expect = 8.221e-88 Identity = 132/293 (45.05%), Postives = 186/293 (63.48%), Query Frame = 3 Query: 861 TKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 ++A+ + + F C C SL G KYI +D+P+C+ C+++ F NTC +C E I D+++L +++ H+HE CF C C SL D FT++DN L C +C+ +FSS C C KI G +K EYAG WHE CFIC C Q IG+KSFIP + C+ CYE+K A +C +C + + K G+TY+ +PWHKECF+CTSC+ L+ FTS++E PYC +CF L+ K+C+ C +PI+ GFGG K+IS D WH CF CS+C S+VG GFF D+++C C Sbjct: 40 SQALKKTTMADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPIT----------GFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322 HSP 2 Score: 129.798 bits (325), Expect = 8.187e-33 Identity = 81/276 (29.35%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C CKE L Y SNDS +C+ + L C EC ELI D + D+ +H F C CD SL + +D+ C C+ N+F + C C + + ++ L + WHE CF+C C + +SF + YC C+++KF+ C C K G Y + WH++CF+C C + + F + C+ C+ + +A+KC C + I G I+++ WH+ CF C+ C L + F ++ C +C Sbjct: 52 FDCDNCKESLYGRKYIQSNDSP--YCIPCYDSLFSNTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322 HSP 3 Score: 104.76 bits (260), Expect = 3.783e-24 Identity = 61/223 (27.35%), Postives = 105/223 (47.09%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH---FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352 H CF C C L D + ++ A+ C + +C CD++++ T+ ++ H F CH C+ + +I D +CV C+E++F C +C + +S +++++ WH++CF+C C L + FT++D YC CF ++ C+ C K G K + +QWH+ CF C+ C+ S+ F P +I C C N Sbjct: 109 HEHCFRCFRCDRSLADEPF--TSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 325
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 273.478 bits (698), Expect = 7.685e-87 Identity = 131/284 (46.13%), Postives = 181/284 (63.73%), Query Frame = 3 Query: 888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739 + F C C SL G KYI +D+P+C+ C+++ F NTC +C E I D+++L +++ H+HE CF C C SL D FT++DN L C +C+ +FSS C C KI G +K EYAG WHE CFIC C Q IG+KSFIP + C+ CYE+K A +C +C + + K G+TY+ +PWHKECF+CTSC+ L+ FTS++E PYC +CF L+ K+C+ C +PI+ GFGG K+IS D WH CF CS+C S+VG GFF D+++C C Sbjct: 2 ADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPIT----------GFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275 HSP 2 Score: 129.028 bits (323), Expect = 5.567e-33 Identity = 81/276 (29.35%), Postives = 129/276 (46.74%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C CKE L Y SNDS +C+ + L C EC ELI D + D+ +H F C CD SL + +D+ C C+ N+F + C C + + ++ L + WHE CF+C C + +SF + YC C+++KF+ C C K G Y + WH++CF+C C + + F + C+ C+ + +A+KC C + I G I+++ WH+ CF C+ C L + F ++ C +C Sbjct: 5 FDCDNCKESLYGRKYIQSNDSP--YCIPCYDSLFSNTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275 HSP 3 Score: 103.605 bits (257), Expect = 3.187e-24 Identity = 61/223 (27.35%), Postives = 106/223 (47.53%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352 H CF C C L D + ++ A+ C + +C CD++++ T+ ++ +H F CH C+ + +I D +CV C+E++F C +C + +S +++++ WH++CF+C C L + FT++D YC CF ++ C+ C K G K + +QWH+ CF C+ C+ S+ F P +I C C N Sbjct: 62 HEHCFRCFRCDRSLADEPF--TSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 278
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: rragcb (Ras-related GTP binding Cb [Source:ZFIN;Acc:ZDB-GENE-070705-380]) HSP 1 Score: 250.751 bits (639), Expect = 3.024e-73 Identity = 122/301 (40.53%), Postives = 172/301 (57.14%), Query Frame = 3 Query: 858 FTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDD 1760 + D E F C C +SL G +YI ++ P C+ C++ NTC +C+E I + K+L+FK+ ++HE CF C C SL + SFT+ ++ L C +C+ +FSS C C KI G K E+ G WHEDCF+C C + IG +SF+P + C+ CYE + +C C++ + K G++Y+G WH+ECFLCT C L+ FTS+ E PYC +CF L+ K+C C I+ GFG K+IS D WH CF CS+C S+VG GFF DQ++C C NDD Sbjct: 436 LPERTDASIMSERFDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAIT----------GFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC----NDD 722 HSP 2 Score: 137.502 bits (345), Expect = 1.779e-33 Identity = 81/275 (29.45%), Postives = 126/275 (45.82%), Query Frame = 3 Query: 711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH-FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520 F+C+ CK L Y + C+ + +L C EC E+I + D ++ EH F C CD SL+ + +D C C+ N+F + C C++ + SK L F WHE CF+C C + SF +N YC C++ +F+ C C K G Y+ G WH +CF+C C+ + + F C+ C+ N +A+KC C+ I G I+++ WH+ CF C+ C L S F ++ C +C Sbjct: 449 FDCRNCKASLYGQQYIKVENEP--HCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQ--GGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC 719
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000009923.1 (SMESG000009923.1) HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0 Identity = 551/564 (97.70%), Postives = 553/564 (98.05%), Query Frame = 3 Query: 90 MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDGNENSTE 1781 MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHV+SLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCD+CHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS GFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDD NENSTE Sbjct: 1 MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVNSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDICHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS----------GFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDANENSTE 554
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000072127.1 (SMESG000072127.1) HSP 1 Score: 480.715 bits (1236), Expect = 4.558e-164 Identity = 249/546 (45.60%), Postives = 330/546 (60.44%), Query Frame = 3 Query: 114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSA--EFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C + H WR C+ C C RH D D ++ + + + ++ MA L WLPIGLQ +E+ F + + E + RK + QIP DR + +H + E Q A F + R+ LG G + +D + C+ C + + +R+ H+A + + H CF+C C E LVD+ YCW ++ +C+RH+ +L RCA CDELI +++T+AM+QE H HFAC CD+SLTG +YIL+DD P C+ C+E+Q+ N C +CS +I CDSKDLSFK+ HWHEKCF C C SLVD+ F TKDN LYC +C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C DC Q IGTKSFIP I C+ CYE K+AQKC KC +IR+ G+TYKG PWHKECF CT C K L+ KFTSKEEKPYC EC+ ELF K+C KCT+PI+ GFGGCKFIS D HWH+ECF C KC S+VGKGF +D+++C CG Sbjct: 18 CRGFKIHGWRKTCLGCDCSVDRH----DITDESPLP-------ILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKL------RKQKLRRQIPVHDRCVEKF-SHLETPTER----QEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQL-AVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKP--YCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPIT----------GFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 528
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 479.945 bits (1234), Expect = 9.150e-164 Identity = 256/547 (46.80%), Postives = 338/547 (61.79%), Query Frame = 3 Query: 114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDN-QEV-CSICDKQFEDEDIFCIRITH--SAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 C + H WR C +C C H D D S CV +D + + + Q Y A +++ L WLPIGL+ E+ FL + + + + R + QIP DR + +H D E Q A F K+R+++ L +E + Q V C C ++ +RI H S + H +CF C CKE LVD+ YCW + ++C RH+GE RCA CDELI N+++T+AMDQE H HFAC CD+SLTG +YIL+++NP C+KC+E +F NTC +C EKI CDSKDLSFK+ HWHEKCF C CH SLVDR F TKD L+C C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C +C Q IG KSFIP + +I C+ CYENK+AQKC KC+E+IR+ G+TYKG PWHKECF CT+C K L+ KFTSK++KPYC +C+ ELF K+C KCT+PI+ GFGGCKFIS HWH+ECF CSKC ++VG+GF ED ++C CG Sbjct: 18 CKGFQLHGWRKTCTACNCSYDIH----DVFDDNIS----CV---LDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFL---SSLPASANPHGVGDDDKSRLRRLRKQIPVQDRRLDKF-SHLDTNVER----QEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSEL-SVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNK--LYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 532
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 377.481 bits (968), Expect = 2.195e-127 Identity = 176/291 (60.48%), Postives = 220/291 (75.60%), Query Frame = 3 Query: 870 MDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 MDQE H HFAC CD+SLTG +YIL+++NP C+KC+E +F NTC +C EKI CDSKDLSFK+ HWHEKCF C CH SLVDR F TKD L+C C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C +C Q IG KSFIP + +I C+ CYENK+AQKC KC+E+IR+ G+TYKG PWHKECF CT+C K L+ KFTSK++KPYC +C+ ELF K+C KCT+PI+ GFGGCKFIS HWH+ECF CSKC ++VG+GF ED ++C CG Sbjct: 1 MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 281 HSP 2 Score: 118.242 bits (295), Expect = 2.358e-29 Identity = 66/229 (28.82%), Postives = 112/229 (48.91%), Query Frame = 3 Query: 1068 HEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGIT---YKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745 H F C C +SL + K+ +C C++ KF++ C+ C + K + + WHE CF C +C+ S+ + F + + C CY+NK A +C C+++ K+G+ Y+G WH+ECFLC C++ + F ++++ C C+ + ++C KCT+ I GG ++ WH ECF C+ C + G F ++D+ C C G Sbjct: 6 HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVF-KAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRR-----------GG---VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYG 219 HSP 3 Score: 106.301 bits (264), Expect = 3.259e-25 Identity = 66/218 (30.28%), Postives = 101/218 (46.33%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343 H CFEC C LVD + ++S +FC + RC C ++ + +++H E F C C + +I RD CV C+EN++ C KC+E I +++K WH++CF C C L FT+KD+ YC +C+ E F+ C C K G K + G+ WH +CF C+ C ++ + F+ I C C Sbjct: 67 HEKCFECFECHTSLVDRPFATKDES--LFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000074426.1 (SMESG000074426.1) HSP 1 Score: 358.221 bits (918), Expect = 7.919e-120 Identity = 162/291 (55.67%), Postives = 214/291 (73.54%), Query Frame = 3 Query: 870 MDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742 M+ + E F+C C SL+G +YIL+D P C+ C+E +F N C +C +KI CDSKDLSFKE HWHE CFLC C +SLVD+ F TKD+ L+CP+C++ KF++ CD CG IF+ G +KYEY G+QWHE+CF+CA+C Q IG KSFIP + + C+ CYENK AQ+C KC+++IRK G+TYKG+PWHKECFLCT C + L+ KFTSK++KP+C +C+ LF K+C KCT+PI+ GFGGCKFIS+ D HWH+ECF CSKC S+VG+GF +D ++C CG Sbjct: 1 MENQNTQEIFSCQNCQKSLSGNRYILKDKLPHCLPCYEEKFANKCDRCKDKIGCDSKDLSFKERHWHEACFLCSSCSESLVDKPFATKDDLLFCPDCYESKFATRCDACGNIFKAGARKYEYRGRQWHENCFVCAECQQPIGAKSFIPRDNQTVCVSCYENKFAQRCTKCNDVIRKGGVTYKGEPWHKECFLCTECHQQLASLKFTSKDDKPFCADCYANLFSKKCTKCTKPIT----------GFGGCKFISLDDRHWHSECFTCSKCDTSLVGRGFLTDKDCIICPECG 281 HSP 2 Score: 107.842 bits (268), Expect = 1.101e-25 Identity = 67/221 (30.32%), Postives = 103/221 (46.61%), Query Frame = 3 Query: 699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352 H +CF C +C E LVD + +D +FC + RC C + + +++H F C C + +I RD+ CV C+EN+F C KC++ I +++K WH++CFLC CH L FT+KD+ +C +C+ FS C C K G K + WH +CF C+ C+ S+ + F+ I C C +N Sbjct: 67 HEACFLCSSCSESLVDKPFATKDD--LLFCPDCYESKFATRCDACGNIFKAGARKYEYRGRQWHENCFVCAECQQPIGAKSFIPRDNQTVCVSCYENKFAQRCTKCNDVIR--KGGVTYKGEPWHKECFLCTECHQQLASLKFTSKDDKPFCADCYANLFSKKCTKCTKPITGFGGCKFISLDDRHWHSECFTCSKCDTSLVGRGFLTDKDCIICPECGQN 283 The following BLAST results are available for this feature:
BLAST of Four and A half lim domains vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Four and A half lim domains vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025952 ID=SMED30025952|Name=Four and A half lim domains|organism=Schmidtea mediterranea sexual|type=transcript|length=1930bpback to top protein sequence of SMED30025952-orf-1 >SMED30025952-orf-1 ID=SMED30025952-orf-1|Name=SMED30025952-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=565bp MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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