Four and A half lim domains

Overview
NameFour and A half lim domains
Smed IDSMED30025952
Length (bp)1930
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Four and A half lim domains (SMED30025952) t-SNE clustered cells

Violin plots show distribution of expression levels for Four and A half lim domains (SMED30025952) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Four and A half lim domains (SMED30025952) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Four and A half lim domains (SMED30025952) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30025952

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025952h1SMcG0022513 dd_Smed_v4_2771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30025952h1SMcG0022513 OX_Smed_1.0.17303ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703])

HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703])

HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703])

HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703])

HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703])

HSP 1 Score: 295.049 bits (754), Expect = 6.836e-95
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 2.024e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 8.929e-27
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5])

HSP 1 Score: 402.519 bits (1033), Expect = 4.662e-133
Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3
Query:  123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            +  H WR  CV C+     H      L    +     V N +         Q    +  +  +  +   W+P GL + ++ +++ ++ N+  P  +     +    R+  +  Q+P  D +S     H   + E K+  +    F  +R+   L  G +    N+ + C  C    E  ++  I          H +CF C+ C++ LVD  YC  ++   I+C RH+ EL   RC+ CDELI   ++TKAM++++H +HF C  CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I  DSKDLS+K+ HWHE CFLC +C  SLVD  F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C  +IGTKSFIP N  + C  CYE K A +C KC ++I   G+TYK +PWH+ECF CT+C   L+  +FTSK+EKPYC  C+ +LF KRC  CT+PI+          G GG KFIS  D HWH +CF C++C  S+VGKGF     +++C  C  AR
Sbjct:   43 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 558          
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5])

HSP 1 Score: 402.519 bits (1033), Expect = 4.662e-133
Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3
Query:  123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            +  H WR  CV C+     H      L    +     V N +         Q    +  +  +  +   W+P GL + ++ +++ ++ N+  P  +     +    R+  +  Q+P  D +S     H   + E K+  +    F  +R+   L  G +    N+ + C  C    E  ++  I          H +CF C+ C++ LVD  YC  ++   I+C RH+ EL   RC+ CDELI   ++TKAM++++H +HF C  CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I  DSKDLS+K+ HWHE CFLC +C  SLVD  F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C  +IGTKSFIP N  + C  CYE K A +C KC ++I   G+TYK +PWH+ECF CT+C   L+  +FTSK+EKPYC  C+ +LF KRC  CT+PI+          G GG KFIS  D HWH +CF C++C  S+VGKGF     +++C  C  AR
Sbjct:   43 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 558          
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5])

HSP 1 Score: 403.675 bits (1036), Expect = 2.185e-132
Identity = 210/545 (38.53%), Postives = 309/545 (56.70%), Query Frame = 3
Query:  123 YTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            +  H WR  CV C+     H      L    +     V N +         Q    +  +  +  +   W+P GL + ++ +++ ++ N+  P  +     +    R+  +  Q+P  D +S     H   + E K+  +    F  +R+   L  G +    N+ + C  C    E  ++  I          H +CF C+ C++ LVD  YC  ++   I+C RH+ EL   RC+ CDELI   ++TKAM++++H +HF C  CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I  DSKDLS+K+ HWHE CFLC +C  SLVD  F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C  +IGTKSFIP N  + C  CYE K A +C KC ++I   G+TYK +PWH+ECF CT+C   L+  +FTSK+EKPYC  C+ +LF KRC  CT+PI+          G GG KFIS  D HWH +CF C++C  S+VGKGF     +++C  C  AR
Sbjct:  135 WKQHPWRKVCVHCKSDRSDH-----ELPPNQALN---VYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 650          
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5])

HSP 1 Score: 401.364 bits (1030), Expect = 5.502e-132
Identity = 199/486 (40.95%), Postives = 293/486 (60.29%), Query Frame = 3
Query:  300 ISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            +  +  +   W+P GL + ++ +++ ++ N+  P  +     +    R+  +  Q+P  D +S     H   + E K+  +    F  +R+   L  G +    N+ + C  C    E  ++  I          H +CF C+ C++ LVD  YC  ++   I+C RH+ EL   RC+ CDELI   ++TKAM++++H +HF C  CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I  DSKDLS+K+ HWHE CFLC +C  SLVD  F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C  +IGTKSFIP N  + C  CYE K A +C KC ++I   G+TYK +PWH+ECF CT+C   L+  +FTSK+EKPYC  C+ +LF KRC  CT+PI+          G GG KFIS  D HWH +CF C++C  S+VGKGF     +++C  C  AR
Sbjct:  149 VPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 613          
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5])

HSP 1 Score: 404.445 bits (1038), Expect = 2.347e-131
Identity = 210/546 (38.46%), Postives = 310/546 (56.78%), Query Frame = 3
Query:  123 YTNHKWRFRCVSCRCPNYRH-VSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFL-RVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            +  H WR  CV C+     H +    +L+  +  G         S   E           +  +  +   W+P GL + ++ +++ ++ N+  P  +     +    R+  +  Q+P  D +S     H   + E K+  +    F  +R+   L  G +    N+ + C  C    E  ++  I          H +CF C+ C++ LVD  YC  ++   I+C RH+ EL   RC+ CDELI   ++TKAM++++H +HF C  CD +LTG +YI+RD+ P+C+KC+E+ F N C +C++ I  DSKDLS+K+ HWHE CFLC +C  SLVD  F +K++ ++C NC+D+ F++ CDGC +IFR G+KK EY GKQWH+ CF CA C  +IGTKSFIP N  + C  CYE K A +C KC ++I   G+TYK +PWH+ECF CT+C   L+  +FTSK+EKPYC  C+ +LF KRC  CT+PI+          G GG KFIS  D HWH +CF C++C  S+VGKGF     +++C  C  AR
Sbjct:  242 WKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPE---------VPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKL----REKQLLLQLPRQD-LSVAYCRHLTSQTERKVYEE----FVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQ--IYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPIT----------GIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAKAR 757          
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302779)

HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138
Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3
Query:   90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            + C+ CKS C  +  H WR  C SC+CP   H  +                      T   H +L  +    A S  E+ +L   W+P G++ +   +  F ++ ++  P    E        R+  I YQ+P  D +S E   H + + E          F  +R+   L    I+     E C+ CD +    ++             H  CF C TC   LVD  YC  +D   ++C RH+ E+   RCA CDELI + ++TKAMD+++H  HF C  CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I  DSKDLS+K+ HWHE CFLC  CH SLVD+ F  K + +YC NC+D +F+S CDGCG++FR G KK EY  +QWHE+CF C  C  +IGTKSFIP   +I C GCYE K A +C KC+++I   G+TYK +PWH+ECF CT C   L+  +FTS++EKPYC ECF ELF KRC  C +PI+          G GG +FIS  D HWH +CF C+ C+AS+VG+GF      ++C  C 
Sbjct:   38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554          

HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40
Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            CA CD  I   +   A  +      +H + F C  C+S L    Y + DD  +C + +       C  C E I       +  +  WH   F C  C +SL  + +  +D++ YC  C++  F++ C+ C KI     K   Y  K WHE CF+C  C+ S+  K F     KI C  CY+ + A +C  C E+ R     + YK   WH+ CF C  C+  +    F  +E++ YC  C+ E F  RC KC + I+            GG   ++  ++ WH ECF C+ C  ++ G+ F  ++++  C  C G
Sbjct:  193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492          

HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25
Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H +CF C  C   LVD  +    D   I+C   +      RC  C E+   +  TK M+   +++H   F C VC +++    +I R+   +C  C+E +F   C KC++ I+  S  +++K   WH +CF C  C+ +L  + FT++D   YC  CF E F+  C  C K      G +   +  + WH DCF+CA C  S+  + FI     I C  C + K
Sbjct:  340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557          

HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17
Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175
            H +CF C  CK  +    +      + I+C   + E    RC +C+++I +   T   ++ +H E F C  C+ +L G ++  RD+ P+C +CF   F   C  C + I+    ++ +SF++ HWH  CF+C  C  SLV R F T    + CP+C  +K 
Sbjct:  401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558          

HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16
Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C+ CN  +   ++   + K+ C   Y      +C  C E+I     T   D  WH   F C  C + L+  ++  +++ PYC +C+  +F   C++C + I  D+K L             S  D HWH  CF C KC  S+V K F  + D++ CG+C  A+
Sbjct:  218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374          
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302781)

HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138
Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3
Query:   90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            + C+ CKS C  +  H WR  C SC+CP   H  +                      T   H +L  +    A S  E+ +L   W+P G++ +   +  F ++ ++  P    E        R+  I YQ+P  D +S E   H + + E          F  +R+   L    I+     E C+ CD +    ++             H  CF C TC   LVD  YC  +D   ++C RH+ E+   RCA CDELI + ++TKAMD+++H  HF C  CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I  DSKDLS+K+ HWHE CFLC  CH SLVD+ F  K + +YC NC+D +F+S CDGCG++FR G KK EY  +QWHE+CF C  C  +IGTKSFIP   +I C GCYE K A +C KC+++I   G+TYK +PWH+ECF CT C   L+  +FTS++EKPYC ECF ELF KRC  C +PI+          G GG +FIS  D HWH +CF C+ C+AS+VG+GF      ++C  C 
Sbjct:   38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554          

HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40
Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            CA CD  I   +   A  +      +H + F C  C+S L    Y + DD  +C + +       C  C E I       +  +  WH   F C  C +SL  + +  +D++ YC  C++  F++ C+ C KI     K   Y  K WHE CF+C  C+ S+  K F     KI C  CY+ + A +C  C E+ R     + YK   WH+ CF C  C+  +    F  +E++ YC  C+ E F  RC KC + I+            GG   ++  ++ WH ECF C+ C  ++ G+ F  ++++  C  C G
Sbjct:  193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492          

HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25
Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H +CF C  C   LVD  +    D   I+C   +      RC  C E+   +  TK M+   +++H   F C VC +++    +I R+   +C  C+E +F   C KC++ I+  S  +++K   WH +CF C  C+ +L  + FT++D   YC  CF E F+  C  C K      G +   +  + WH DCF+CA C  S+  + FI     I C  C + K
Sbjct:  340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557          

HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17
Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175
            H +CF C  CK  +    +      + I+C   + E    RC +C+++I +   T   ++ +H E F C  C+ +L G ++  RD+ P+C +CF   F   C  C + I+    ++ +SF++ HWH  CF+C  C  SLV R F T    + CP+C  +K 
Sbjct:  401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558          

HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16
Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C+ CN  +   ++   + K+ C   Y      +C  C E+I     T   D  WH   F C  C + L+  ++  +++ PYC +C+  +F   C++C + I  D+K L             S  D HWH  CF C KC  S+V K F  + D++ CG+C  A+
Sbjct:  218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374          
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0306704)

HSP 1 Score: 416.772 bits (1070), Expect = 1.253e-138
Identity = 226/558 (40.50%), Postives = 312/558 (55.91%), Query Frame = 3
Query:   90 MDCKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            + C+ CKS C  +  H WR  C SC+CP   H  +                      T   H +L  +    A S  E+ +L   W+P G++ +   +  F ++ ++  P    E        R+  I YQ+P  D +S E   H + + E          F  +R+   L    I+     E C+ CD +    ++             H  CF C TC   LVD  YC  +D   ++C RH+ E+   RCA CDELI + ++TKAMD+++H  HF C  CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I  DSKDLS+K+ HWHE CFLC  CH SLVD+ F  K + +YC NC+D +F+S CDGCG++FR G KK EY  +QWHE+CF C  C  +IGTKSFIP   +I C GCYE K A +C KC+++I   G+TYK +PWH+ECF CT C   L+  +FTS++EKPYC ECF ELF KRC  C +PI+          G GG +FIS  D HWH +CF C+ C+AS+VG+GF      ++C  C 
Sbjct:   38 VRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 554          

HSP 2 Score: 155.606 bits (392), Expect = 3.256e-40
Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            CA CD  I   +   A  +      +H + F C  C+S L    Y + DD  +C + +       C  C E I       +  +  WH   F C  C +SL  + +  +D++ YC  C++  F++ C+ C KI     K   Y  K WHE CF+C  C+ S+  K F     KI C  CY+ + A +C  C E+ R     + YK   WH+ CF C  C+  +    F  +E++ YC  C+ E F  RC KC + I+            GG   ++  ++ WH ECF C+ C  ++ G+ F  ++++  C  C G
Sbjct:  193 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 492          

HSP 3 Score: 110.923 bits (276), Expect = 3.341e-25
Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H +CF C  C   LVD  +    D   I+C   +      RC  C E+   +  TK M+   +++H   F C VC +++    +I R+   +C  C+E +F   C KC++ I+  S  +++K   WH +CF C  C+ +L  + FT++D   YC  CF E F+  C  C K      G +   +  + WH DCF+CA C  S+  + FI     I C  C + K
Sbjct:  340 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557          

HSP 4 Score: 85.8853 bits (211), Expect = 3.213e-17
Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175
            H +CF C  CK  +    +      + I+C   + E    RC +C+++I +   T   ++ +H E F C  C+ +L G ++  RD+ P+C +CF   F   C  C + I+    ++ +SF++ HWH  CF+C  C  SLV R F T    + CP+C  +K 
Sbjct:  401 HENCFCCCVCKTAIGTKSFI--PREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558          

HSP 5 Score: 83.9593 bits (206), Expect = 1.380e-16
Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C+ CN  +   ++   + K+ C   Y      +C  C E+I     T   D  WH   F C  C + L+  ++  +++ PYC +C+  +F   C++C + I  D+K L             S  D HWH  CF C KC  S+V K F  + D++ CG+C  A+
Sbjct:  218 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 374          
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0332703)

HSP 1 Score: 406.757 bits (1044), Expect = 4.333e-135
Identity = 219/540 (40.56%), Postives = 302/540 (55.93%), Query Frame = 3
Query:  141 RFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            R  C SC+CP   H  +                      T   H +L  +    A S  E+ +L   W+P G++ +   +  F ++ ++  P    E        R+  I YQ+P  D +S E   H + + E          F  +R+   L    I+     E C+ CD +    ++             H  CF C TC   LVD  YC  +D   ++C RH+ E+   RCA CDELI + ++TKAMD+++H  HF C  CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I  DSKDLS+K+ HWHE CFLC  CH SLVD+ F  K + +YC NC+D +F+S CDGCG++FR G KK EY  +QWHE+CF C  C  +IGTKSFIP   +I C GCYE K A +C KC+++I   G+TYK +PWH+ECF CT C   L+  +FTS++EKPYC ECF ELF KRC  C +PI+          G GG +FIS  D HWH +CF C+ C+AS+VG+GF      ++C  C 
Sbjct:   26 RKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGA----CSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 524          

HSP 2 Score: 156.377 bits (394), Expect = 1.150e-40
Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            CA CD  I   +   A  +      +H + F C  C+S L    Y + DD  +C + +       C  C E I       +  +  WH   F C  C +SL  + +  +D++ YC  C++  F++ C+ C KI     K   Y  K WHE CF+C  C+ S+  K F     KI C  CY+ + A +C  C E+ R     + YK   WH+ CF C  C+  +    F  +E++ YC  C+ E F  RC KC + I+            GG   ++  ++ WH ECF C+ C  ++ G+ F  ++++  C  C G
Sbjct:  163 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 462          

HSP 3 Score: 110.923 bits (276), Expect = 2.282e-25
Identity = 70/224 (31.25%), Postives = 108/224 (48.21%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H +CF C  C   LVD  +    D   I+C   +      RC  C E+   +  TK M+   +++H   F C VC +++    +I R+   +C  C+E +F   C KC++ I+  S  +++K   WH +CF C  C+ +L  + FT++D   YC  CF E F+  C  C K      G +   +  + WH DCF+CA C  S+  + FI     I C  C + K
Sbjct:  310 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 527          

HSP 4 Score: 85.8853 bits (211), Expect = 2.727e-17
Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175
            H +CF C  CK  +    +      + I+C   + E    RC +C+++I +   T   ++ +H E F C  C+ +L G ++  RD+ P+C +CF   F   C  C + I+    ++ +SF++ HWH  CF+C  C  SLV R F T    + CP+C  +K 
Sbjct:  371 HENCFCCCVCKTAIGTKSF--IPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 528          

HSP 5 Score: 84.3445 bits (207), Expect = 8.501e-17
Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C+ CN  +   ++   + K+ C   Y      +C  C E+I     T   D  WH   F C  C + L+  ++  +++ PYC +C+  +F   C++C + I  D+K L             S  D HWH  CF C KC  S+V K F  + D++ CG+C  A+
Sbjct:  188 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 344          
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302780)

HSP 1 Score: 405.601 bits (1041), Expect = 2.654e-134
Identity = 219/540 (40.56%), Postives = 302/540 (55.93%), Query Frame = 3
Query:  141 RFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL--QDYKAAISMAESNNL--VWLPIGLQQAEISD--FLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            R  C SC+CP   H  +                      T   H +L  +    A S  E+ +L   W+P G++ +   +  F ++ ++  P    E        R+  I YQ+P  D +S E   H + + E          F  +R+   L    I+     E C+ CD +    ++             H  CF C TC   LVD  YC  +D   ++C RH+ E+   RCA CDELI + ++TKAMD+++H  HF C  CD SLTG +Y++RDD+P+C+KC+EN F NTC +C++ I  DSKDLS+K+ HWHE CFLC  CH SLVD+ F  K + +YC NC+D +F+S CDGCG++FR G KK EY  +QWHE+CF C  C  +IGTKSFIP   +I C GCYE K A +C KC+++I   G+TYK +PWH+ECF CT C   L+  +FTS++EKPYC ECF ELF KRC  C +PI+          G GG +FIS  D HWH +CF C+ C+AS+VG+GF      ++C  C 
Sbjct:   55 RKTCQSCKCPREAHAIY-------------------QQQTTNVHERLGFKLVSPADSGVEARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEG----ACSRERQISYQLPKQD-LSLEHCKHLEVQHEASF-----EDFVTARNEIALDIAYIKDAPYDEHCAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDK--VYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPIT----------GIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCA 553          

HSP 2 Score: 157.147 bits (396), Expect = 9.940e-41
Identity = 93/315 (29.52%), Postives = 144/315 (45.71%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQ-----EFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            CA CD  I   +   A  +      +H + F C  C+S L    Y + DD  +C + +       C  C E I       +  +  WH   F C  C +SL  + +  +D++ YC  C++  F++ C+ C KI     K   Y  K WHE CF+C  C+ S+  K F     KI C  CY+ + A +C  C E+ R     + YK   WH+ CF C  C+  +    F  +E++ YC  C+ E F  RC KC + I+            GG   ++  ++ WH ECF C+ C  ++ G+ F  ++++  C  C G
Sbjct:  192 CAHCDNEIAAGELVVAAPKFVESVMWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDK-DWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITS-----------GG---VTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFG 491          

HSP 3 Score: 110.923 bits (276), Expect = 3.570e-25
Identity = 71/224 (31.70%), Postives = 108/224 (48.21%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H +CF C  C   LVD  +    D   I+C   +      RC  C E  V +  TK M+   +++H   F C VC +++    +I R+   +C  C+E +F   C KC++ I+  S  +++K   WH +CF C  C+ +L  + FT++D   YC  CF E F+  C  C K      G +   +  + WH DCF+CA C  S+  + FI     I C  C + K
Sbjct:  339 HEACFLCFKCHLSLVDKQFGAKADK--IYCGNCYDAQFASRCDGCGE--VFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 556          

HSP 4 Score: 85.5001 bits (210), Expect = 3.991e-17
Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKF 1175
            H +CF C  CK  +    +      + I+C   + E    RC +C+++I +   T   ++ +H E F C  C+ +L G ++  RD+ P+C +CF   F   C  C + I+    ++ +SF++ HWH  CF+C  C  SLV R F T    + CP+C  +K 
Sbjct:  400 HENCFCCCVCKTAIGTKSF--IPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYK-NEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 557          

HSP 5 Score: 85.1149 bits (209), Expect = 5.149e-17
Identity = 52/170 (30.59%), Postives = 80/170 (47.06%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS-DTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C+ CN  +   ++   + K+ C   Y      +C  C E+I     T   D  WH   F C  C + L+  ++  +++ PYC +C+  +F   C++C + I  D+K L             S  D HWH  CF C KC  S+V K F  + D++ CG+C  A+
Sbjct:  217 WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDL-------------SYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQ 373          
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121])

HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93
Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C  CH SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C +C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++ PYC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33
Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y   +++   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + C +C + I   S+ +  K   WHE CF C  C   +  +SF  KDN  YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23
Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D++ +CV C+E QF   C +C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121])

HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93
Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C  CH SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C +C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++ PYC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33
Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y   +++   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + C +C + I   S+ +  K   WHE CF C  C   +  +SF  KDN  YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23
Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D++ +CV C+E QF   C +C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121])

HSP 1 Score: 289.271 bits (739), Expect = 6.192e-93
Identity = 136/285 (47.72%), Postives = 181/285 (63.51%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LRD+NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C  CH SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C +C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++ PYC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 AERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 130.954 bits (328), Expect = 1.105e-33
Identity = 81/275 (29.45%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y   +++   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + C +C + I   S+ +  K   WHE CF C  C   +  +SF  KDN  YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLRDENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 100.908 bits (250), Expect = 2.948e-23
Identity = 65/220 (29.55%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D++ +CV C+E QF   C +C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HDDCFHCFKCHRSLVDKPF--STKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49])

HSP 1 Score: 284.263 bits (726), Expect = 5.968e-91
Identity = 135/285 (47.37%), Postives = 177/285 (62.11%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LR+DNP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE CF C  C  SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C  C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++  YC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF    D ++C  CG
Sbjct:    2 TERYDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECG 276          

HSP 2 Score: 137.117 bits (344), Expect = 7.817e-36
Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y    D+   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + CH+C + I   S+ +  K   WHE CF C  C   +  +SF  KDN+ YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F +  +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLREDNP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275          

HSP 3 Score: 103.219 bits (256), Expect = 6.205e-24
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  CK  LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D++ +CV C+E QF   C  C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEDCFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49])

HSP 1 Score: 284.263 bits (726), Expect = 5.968e-91
Identity = 135/285 (47.37%), Postives = 177/285 (62.11%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LR+DNP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE CF C  C  SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C  C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++  YC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF    D ++C  CG
Sbjct:    2 TERYDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECG 276          

HSP 2 Score: 137.117 bits (344), Expect = 7.817e-36
Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y    D+   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + CH+C + I   S+ +  K   WHE CF C  C   +  +SF  KDN+ YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F +  +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLREDNP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275          

HSP 3 Score: 103.219 bits (256), Expect = 6.205e-24
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  CK  LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D++ +CV C+E QF   C  C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEDCFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl2 (four and a half LIM domains 2 [Source:NCBI gene;Acc:100145283])

HSP 1 Score: 293.893 bits (751), Expect = 1.574e-94
Identity = 138/285 (48.42%), Postives = 181/285 (63.51%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C  SL G KY+LR++NP+CVKC+E+ + N+C +C + I CD KDLS+K+ HWHE CF C  C  SLVD+ F  KD +L C  C+  ++SS C  C K    G +K EY G  WHE CFIC+ C Q IGTKSFIP + +  C+ CYE + A  C +C + I   G+TY+  PWHKECF+CT C+K LS  +FTS++E  YC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH++CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPIS----------GLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 130.568 bits (327), Expect = 2.239e-33
Identity = 80/276 (28.99%), Postives = 134/276 (48.55%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  CKE L    Y    ++   +C++ +  L    C EC + I    KD +   D+ +H   F C  C  SL    +  +D++  C +C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++F+  C  C K   TG     Y  + WH++CFIC  C + +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 FDCHYCKESLFGKKYLLREENP--YCVKCYESLYSNSCEECKKPIGCDGKDLS-YKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTG--GVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 107.457 bits (267), Expect = 2.893e-25
Identity = 66/220 (30.00%), Postives = 107/220 (48.64%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H SCF C  CK+ LVD  +  +   + + C   +      +C+EC + I+    T+ M+     +H   F C  C   +    +I +D+  FCV C+E QF   C +C + I+  +  +++++  WH++CF+C  C   L  + FT++D + YC NCF   ++  C GC        G K   +  +QWH DCF C  C  S+  + F+     I C  C
Sbjct:   62 HESCFHCFQCKKSLVDKPF--AAKDEHLICTECYSNEYSSKCSECKKTIMPG--TRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAIT--TGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl5 (four and a half LIM domains 5 [Source:NCBI gene;Acc:100144736])

HSP 1 Score: 272.707 bits (696), Expect = 2.343e-86
Identity = 131/286 (45.80%), Postives = 176/286 (61.54%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748
            E F C  C  SL G KY L+DD P+C+KCF + F N C +C + I C+SKDL++K+ HWHE CF CD C  SLV++ F  KD  L C  C+  ++SS C GC      G +K EY G  WHE CF+C  C + +G K FIP   KI C+ CYE + A +C  C + I K G++++   WH+ECF+CTSC+K L   K TS++E PYC +CF  L+ K+C  C +PI+          G GG K+IS  D  WH++CF C+KC  S+VG+ F   ED ++C SCG A
Sbjct:    4 EIFDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPIT----------GQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCGLA 279          

HSP 2 Score: 135.961 bits (341), Expect = 3.647e-35
Identity = 77/279 (27.60%), Postives = 135/279 (48.39%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C  CKE L    Y   +D    +C++ F  L    C  C + I  N       D  +H   F C  CD SL    +  +D+   C++C+  ++ + C  C   I   S+ + +   +WHE CF+C  C + + ++ F  K++ +YC  C++++F++ C  C K    G     +  +QWH +CF+C  C +++  +     +    C+ C++N +A+KC  C++ I   G    I+++   WH +CF C  C K L   KF + E+   C  C + +
Sbjct:    6 FDCFHCKESLYGKKYTLKDD--IPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKG--GLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCGLAI 280          

HSP 3 Score: 85.8853 bits (211), Expect = 6.186e-18
Identity = 50/156 (32.05%), Postives = 85/156 (54.49%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCD--SKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160
            H +CF C++C+E + +  +    +SK I+C+  + +    +C  C + I     +   +Q++H E F C  C  +L G K   RD++P+CV CF+N +   C  C++ I+    +K +SF++  WH  CF C  C  SLV   F T ++ + CP+C
Sbjct:  124 HETCFVCQSCREPVGNKPF-IPKESK-IYCMPCYEKQFANQCKSCRKAITKGGLS-FQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276          
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: shroom3 (shroom family member 3 [Source:Xenbase;Acc:XB-GENE-490224])

HSP 1 Score: 265.774 bits (678), Expect = 9.051e-84
Identity = 129/283 (45.58%), Postives = 178/283 (62.90%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            E F C  C  SL G KYI  ++ P+C+ C+++ F NTC +C E I  D ++L +++ H+HE CF C  C  SL D  FT +D  L C +C+  +FSS C  C K    G +K EY G+ WHE CFIC  C Q IG++SFIP N    CI CYE+K A +C  C + + K G+TY+ +PWHKECF+CT C+  L+  +FTS++EKPYC +CF  L+ K+C  CT+PI+          GFGG K++S  + HWH  CFNCS+C  S+VGKGF    + ++C +C
Sbjct:    3 EPFDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPIT----------GFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275          

HSP 2 Score: 127.487 bits (319), Expect = 3.251e-32
Identity = 81/279 (29.03%), Postives = 126/279 (45.16%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNK-DFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C +CKE L    Y    +    +C+  +  L    C EC ELI +        D+ +H   F C  CD SL    +  +D+   C  C+ N+F + C  C + +   S+ L +    WHE CF+C+ C   +  RSF  ++   YC  C++ K +  C  C K    G     Y  + WH++CF+C  C   +  + F   + K  CI C+ N +A+KC  C++ I   G    ++++   WH  CF C+ C   L    F    E   C  C  +L
Sbjct:    5 FDCDSCKESLYGRKYIQMEEGP--YCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKG--GVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRACNSDL 279          

HSP 3 Score: 118.242 bits (295), Expect = 5.253e-29
Identity = 66/224 (29.46%), Postives = 106/224 (47.32%), Query Frame = 3
Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            F CD C +SL  R +   +   YC  C+D  F++ CD C ++     ++  Y  + +HE CF C  C+ S+  + F   + ++ C  CY N+ + KC  C + +      + Y G  WH+ CF+C SCQ+ +    F  + +  YC  C+      RC  C          K L KG      ++  D+ WH ECF C+ C+  + G+ F  Q+++  C  C G
Sbjct:    5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCK---------KSLTKGG-----VTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFG 214          
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334])

HSP 1 Score: 223.016 bits (567), Expect = 3.703e-67
Identity = 110/286 (38.46%), Postives = 155/286 (54.20%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C + L G KYI +D +  CVKCF+    NTC +C + I  DSK+L +K  +WH+ CF C  C+  L +  F  KDN + C  C   + S  C GC K  + G +  EY G  WHE+CF C++C Q+IG+ SF P    + C+ C+E K A+ C KC+  I   GITY+  PWH +CF+C +C K L+  +FT+ E+  YC +C+     K+C  C  PI+          GFG G   ++   + WH  CF C KC  ++  K F    +Q+ C  C 
Sbjct:   18 AERFDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPIT----------GFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCA 293          

HSP 2 Score: 126.716 bits (317), Expect = 9.022e-32
Identity = 77/280 (27.50%), Postives = 131/280 (46.79%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C  C+  L    Y   +      C++ F ++    CAEC + I V+       ++ +H   F C  C   L   ++I +D+   C KC   +    C  C ++I    +++ +K   WHE+CF C  C  ++   SF  K   +YC  C ++KF+  C  C     +G   Y+   + WH DCF+C  C++ +  + F  +     C+ CY++  A+KC  C+  I   G     + Y+G+ WH+ CF C  C   L+  +F    E+ YC +C  ++
Sbjct:   21 FDCHYCRAPLQGKKYIEKDGHNT--CVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQ--DQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 296          

HSP 3 Score: 65.4698 bits (158), Expect = 5.780e-11
Identity = 46/183 (25.14%), Postives = 79/183 (43.17%), Query Frame = 3
Query:  621 CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKD---LSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160
            CS C KQ +       R       A H  CF C  CK+ +    +        ++C+    +     C +C+  I +   T   DQ +H + F C  C   L G ++   +D+ +CV C+++     C  C+  I+   K    ++++   WHE CF C  C  +L ++ F   +  +YC +C
Sbjct:  117 CSGCHKQIQPGG----RNVEYKGSAWHEECFTCSNCKQAIGSGSF--FPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGIT-YQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 292          
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334])

HSP 1 Score: 222.631 bits (566), Expect = 4.450e-67
Identity = 110/286 (38.46%), Postives = 155/286 (54.20%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C + L G KYI +D +  CVKCF+    NTC +C + I  DSK+L +K  +WH+ CF C  C+  L +  F  KDN + C  C   + S  C GC K  + G +  EY G  WHE+CF C++C Q+IG+ SF P    + C+ C+E K A+ C KC+  I   GITY+  PWH +CF+C +C K L+  +FT+ E+  YC +C+     K+C  C  PI+          GFG G   ++   + WH  CF C KC  ++  K F    +Q+ C  C 
Sbjct:   13 AERFDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPIT----------GFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCA 288          

HSP 2 Score: 126.331 bits (316), Expect = 9.150e-32
Identity = 77/280 (27.50%), Postives = 131/280 (46.79%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C  C+  L    Y   +      C++ F ++    CAEC + I V+       ++ +H   F C  C   L   ++I +D+   C KC   +    C  C ++I    +++ +K   WHE+CF C  C  ++   SF  K   +YC  C ++KF+  C  C     +G   Y+   + WH DCF+C  C++ +  + F  +     C+ CY++  A+KC  C+  I   G     + Y+G+ WH+ CF C  C   L+  +F    E+ YC +C  ++
Sbjct:   16 FDCHYCRAPLQGKKYIEKDGHNT--CVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQ--DQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 291          

HSP 3 Score: 65.4698 bits (158), Expect = 4.578e-11
Identity = 46/183 (25.14%), Postives = 79/183 (43.17%), Query Frame = 3
Query:  621 CSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKD---LSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160
            CS C KQ +       R       A H  CF C  CK+ +    +        ++C+    +     C +C+  I +   T   DQ +H + F C  C   L G ++   +D+ +CV C+++     C  C+  I+   K    ++++   WHE CF C  C  +L ++ F   +  +YC +C
Sbjct:  112 CSGCHKQIQPGG----RNVEYKGSAWHEECFTCSNCKQAIGSGSF--FPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGIT-YQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 287          
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762])

HSP 1 Score: 290.812 bits (743), Expect = 1.662e-93
Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYIL+++NP CV CFE  + NTC +C   I CD KDLS+K+ HWHE CF C  C  SLVD+ F  K+  L C +C+  ++SS C  C K    G +K EY G  WHE CF C  C Q IGTKSFIP   +  C+ CYE ++A +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT+++E PYC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 119.013 bits (297), Expect = 2.125e-29
Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    ++    C+  F EL    C EC   I    KD +   D+ +H   F C  C SSL    +  +++   C  C+ N++ + C +C + I   ++ + +K   WHE CF C  C   +  +SF  K+N  +C  C++++++  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 103.99 bits (258), Expect = 3.041e-24
Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  +   + + C   +      +C EC + I+    T+ M+     +H   F C  C   +    +I +++  FCV C+E Q+   C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D + YC  CF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762])

HSP 1 Score: 290.812 bits (743), Expect = 1.662e-93
Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYIL+++NP CV CFE  + NTC +C   I CD KDLS+K+ HWHE CF C  C  SLVD+ F  K+  L C +C+  ++SS C  C K    G +K EY G  WHE CF C  C Q IGTKSFIP   +  C+ CYE ++A +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT+++E PYC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 119.013 bits (297), Expect = 2.125e-29
Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    ++    C+  F EL    C EC   I    KD +   D+ +H   F C  C SSL    +  +++   C  C+ N++ + C +C + I   ++ + +K   WHE CF C  C   +  +SF  K+N  +C  C++++++  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 103.99 bits (258), Expect = 3.041e-24
Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  +   + + C   +      +C EC + I+    T+ M+     +H   F C  C   +    +I +++  FCV C+E Q+   C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D + YC  CF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092])

HSP 1 Score: 270.396 bits (690), Expect = 1.261e-85
Identity = 125/286 (43.71%), Postives = 177/286 (61.89%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748
            E F C  C+ SL G KYI  D  P+CV C++N F NTC +C + I  DS++L +++ H+HE CF C  C  SL D  FT +D+ L C  C+   FSS C  CG+    G +K EY G+ WHE CF+C+ C Q +G++SF+P  G   C+ CYENK A +C +CS+ + + G+TY+  PWH+EC +CT C+  L+  +FTS+++ PYC  CF ELF  +C  C +PI+          G GG K++S  D HWH  CF+C++C  S+VG+GF    DQ++C  C  A
Sbjct:    3 EAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPIT----------GLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278          

HSP 2 Score: 119.783 bits (299), Expect = 1.315e-29
Identity = 76/275 (27.64%), Postives = 110/275 (40.00%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y  ++     +C+  +       CAEC +LI +        D+ FH   F C  C  SL    +  +D    C +C+   F + C  C E +   S+ L +    WHE CFLC  C   L  RSF       YC  C++ KF+  C  C K    G     Y  + WH +C +C  C   +  + F   +    C+ C+    A KC  C   I   G    ++++   WH  CF C  C   L    F    ++  C  C
Sbjct:    5 FDCAKCNESLYGRKYIQTDSGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275          
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092])

HSP 1 Score: 263.077 bits (671), Expect = 1.018e-82
Identity = 124/286 (43.36%), Postives = 176/286 (61.54%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748
            E F C  C+ SL G KYI  D  P+CV C++N F NTC +C + I  DS++L +++ H+HE CF C  C  SL D  FT +D+ L C  C+   FSS C  CG+    G +K EY G+ WHE CF+C+ C Q +G++SF+P  G   C+ CYENK A +C +CS+ + + G+TY+  PWH+EC +CT C+  L+  +FTS+++ PYC  CF ELF  +C  C +PI+            GG K++S  D HWH  CF+C++C  S+VG+GF    DQ++C  C  A
Sbjct:    3 EAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGG-KYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 287          

HSP 2 Score: 113.62 bits (283), Expect = 1.959e-27
Identity = 75/284 (26.41%), Postives = 109/284 (38.38%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-------------ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y  ++     +C+  +       CAEC +LI +        D+ FH   F C  C  SL    +  +D    C +C+   F + C  C E +   S+ L +    WHE CFLC  C   L  RSF       YC  C++ KF+  C  C K    G     Y  + WH +C +C  C   +  + F   +    C+ C+    A KC  C   I                 ++++   WH  CF C  C   L    F    ++  C  C
Sbjct:    5 FDCAKCNESLYGRKYIQTDSGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 284          
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192])

HSP 1 Score: 256.914 bits (655), Expect = 2.302e-80
Identity = 125/290 (43.10%), Postives = 169/290 (58.28%), Query Frame = 3
Query:  894 HFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDG 1763
             F C  C SSL G KY+L+DDN +C+ C++  F N C +C E I  DSKDL +K  HWHE CF C+ CH SLV++ F  KD+ L C +C+  + SS C  C +    G +K E+ G  WHE CF+C  C Q IGTK  I       C+ C+E + A  C  C ++I   GIT++   WHKECFLC+ C+K L E  F SK++ P+C +C+  L+ K+C  CT+PI+          G  G KFI   D  WH+ECFNC KC  S+VG+GF     +++C  CG   + D 
Sbjct:    5 QFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPIT----------GLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA 284          

HSP 2 Score: 121.324 bits (303), Expect = 3.540e-30
Identity = 74/276 (26.81%), Postives = 136/276 (49.28%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR----KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C+ C   L+   Y   +D+  ++C+  +  +    C +C E I +  KD     ++ +H   F C+ C  SL    ++ +DD   C  C+ N+  + C  C   I   S+ + FK  +WHE CF+C+ C   +  +   +K++  YC  CF+++F+  C+ C K+  +G     +  + WH++CF+C+ C + +  ++F+  +    C+ CY + +A+KC  C++ I        I ++   WH ECF C  C   L    F +   +  C +C
Sbjct:    6 FDCQYCTSSLIGKKYVLKDDN--LYCISCYDRIFSNYCEQCKEPIESDSKDLCYK-NRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276          

HSP 3 Score: 61.2326 bits (147), Expect = 8.137e-10
Identity = 43/156 (27.56%), Postives = 73/156 (46.79%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISC--DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNC 1160
            H +CF C+ C++ +        +     +C+  F +     C  C ++I +   T   DQ +H E F C  C   L    ++ +DD PFC+ C+ + +   C  C++ I+    +K + F++  WH +CF C  C  SLV   F T +  + C  C
Sbjct:  124 HETCFVCEHCRQPIGTKPL--ISKESGNYCVPCFEKEFAHYCNFCKKVITSGGIT-FRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276          
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q14192|FHL2_HUMAN (Four and a half LIM domains protein 2 OS=Homo sapiens OX=9606 GN=FHL2 PE=1 SV=3)

HSP 1 Score: 295.049 bits (754), Expect = 3.283e-94
Identity = 138/285 (48.42%), Postives = 184/285 (64.56%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYILR+++P+CV CFE  F NTC +C + I CD KDLS+K+ HWHE CF C  C +SLVD+ F  K++ L C +C+  ++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE +HA +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT++++  YC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.479 bits (306), Expect = 9.718e-30
Identity = 77/276 (27.90%), Postives = 130/276 (47.10%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    +S   +C+  F  L    C EC + I    KD +   D+ +H   F C  C +SL    +  ++D   C  C+ N++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KDN  +C  C++++ +  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLFGKKYILREESP--YCVVCFETLFANTCEECGKPIGCDCKDLS-YKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 4.288e-26
Identity = 66/220 (30.00%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   +      +C EC + I+    T+ M+     +H   F CH C   +    +I +D+  FCV C+E Q    C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D++ YC NCF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSQCRNSLVDKPFAAKEDQ--LLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPIT--TGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O70433|FHL2_MOUSE (Four and a half LIM domains protein 2 OS=Mus musculus OX=10090 GN=Fhl2 PE=1 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 1.162e-92
Identity = 137/285 (48.07%), Postives = 179/285 (62.81%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYIL+++NP CV CFE  + NTC +C   I CD KDLS+K+ HWHE CF C  C  SLVD+ F  K+  L C +C+  ++SS C  C K    G +K EY G  WHE CF C  C Q IGTKSFIP   +  C+ CYE ++A +C +C + I   G+TY+  PWHKECF+CT+C+K LS  +FT+++E PYC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 119.013 bits (297), Expect = 1.486e-28
Identity = 76/276 (27.54%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    ++    C+  F EL    C EC   I    KD +   D+ +H   F C  C SSL    +  +++   C  C+ N++ + C +C + I   ++ + +K   WHE CF C  C   +  +SF  K+N  +C  C++++++  C  C K   TG     Y  + WH++CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 103.99 bits (258), Expect = 2.127e-23
Identity = 62/220 (28.18%), Postives = 103/220 (46.82%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  +   + + C   +      +C EC + I+    T+ M+     +H   F C  C   +    +I +++  FCV C+E Q+   C +C + I+  +  ++++E  WH++CF+C  C   L  + FT +D + YC  CF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O35115|FHL2_RAT (Four and a half LIM domains protein 2 OS=Rattus norvegicus OX=10116 GN=Fhl2 PE=1 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.922e-92
Identity = 136/285 (47.72%), Postives = 179/285 (62.81%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KYIL+++NP CV CFE  + NTC +C   I CD KDLS+K+ HWHE CF C  C  SLVD+ F  K+  L C +C+  ++SS C  C K    G +K EY G  WHE CF C  C Q IGTKSFIP   +  C+ CYE ++A +C +C + I   G+TY+  PWH+ECF+CT+C+K LS  +FT+++E PYC  CF +L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 119.013 bits (297), Expect = 1.337e-28
Identity = 76/276 (27.54%), Postives = 128/276 (46.38%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y    ++    C+  F EL    C EC   I    KD +   D+ +H   F C  C SSL    +  +++   C  C+ N++ + C +C + I   ++ + +K   WHE CF C  C   +  +SF  K+N  +C  C++++++  C  C K   TG     Y  + WH +CF+C  C + +  + F   +    C+ C+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCNESLYGKKYILKEENP--HCVACFEELYANTCEECGTPIGCDCKDLS-YKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 102.449 bits (254), Expect = 7.185e-23
Identity = 61/220 (27.73%), Postives = 102/220 (46.36%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C   LVD  +  +   + + C   +      +C EC + I+    T+ M+     +H   F C  C   +    +I +++  FCV C+E Q+   C +C + I+  +  +++++  WH +CF+C  C   L  + FT +D + YC  CF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEGCFHCSRCGSSLVDKPF--AAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPIT--TGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q2KI95|FHL2_BOVIN (Four and a half LIM domains protein 2 OS=Bos taurus OX=9913 GN=FHL2 PE=2 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 2.206e-91
Identity = 135/285 (47.37%), Postives = 182/285 (63.86%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F CH C+ SL G KY+LR++ P+CV CFE  F +TC +C + I CD KDLS+K+ HWHE CF C  C  SLVD+ F  K++ L C +C+ +++SS C  C K    G +K EY G  WHE CFIC  C Q IGTKSFIP + +  C+ CYE ++A +C +C + I   G+TY+  PWH+ECF+CT+C+K LS  +FTS++E  YC  CF +L+ K+C  C  PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERFDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPIS----------GLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG 276          

HSP 2 Score: 122.865 bits (307), Expect = 5.702e-30
Identity = 75/276 (27.17%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVN--KDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C++ L    Y    +    +C+  F  L    C EC +LI    KD +   D+ +H   F C  C  SL    +  ++D   C  C+  ++ + C +C + I   ++ + +K   WHE CF+C  C   +  +SF  KD+  +C  C++ +++  C  C K   TG     Y  + WH +CF+C  C + +  + F   +    C+GC+ + +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C +C
Sbjct:    5 FDCHHCEDSLFGRKYVLREEQP--YCVACFEALFASTCEECGKLIGCDCKDLS-YKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTG--GVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          

HSP 3 Score: 112.079 bits (279), Expect = 3.440e-26
Identity = 66/220 (30.00%), Postives = 105/220 (47.73%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H +CF C  C+  LVD  +    D   + C   + +    RC EC + I+    T+ M+     +H   F CH C   +    +I +D   FCV C+E Q+   C +C + I+  +  ++++E  WH +CF+C  C   L  + FT++D + YC  CF + ++  C GC        G K   +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEACFHCSRCRGSLVDKPFAAKEDQ--LLCTDCYSQEYSSRCQECKKSIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPIT--TGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275          
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q3ZBI6|FHL3_BOVIN (Four and a half LIM domains protein 3 OS=Bos taurus OX=9913 GN=FHL3 PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 1.010e-86
Identity = 128/286 (44.76%), Postives = 178/286 (62.24%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGA 1748
            E F C  C  SL G KYI  DD P+CV C++N F NTC +C + I  DS++L +++ H+HE CF C  C  SL D  FT +D+ L C +C+   FSS C  CG+    G +K EY G+ WHE CF+C+ C Q +G++SF+P  G   C+ CYENK A +C +CS+ + + G+TY+  PWH+EC +CT CQ  L+  +FTS+E+ PYC  CF ELF  +C  C +PI+          G GG K++S  D HWH  CF+C++C  S+VG+GF    DQ++C  C  A
Sbjct:    3 EAFDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPIT----------GLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278          

HSP 2 Score: 120.168 bits (300), Expect = 5.566e-29
Identity = 77/275 (28.00%), Postives = 110/275 (40.00%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM-DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  C E L    Y  ++D    +C+  +       CAEC +LI +        D+ FH   F C  C  SL    +  +D    C  C+ + F + C  C E +   S+ L +    WHE CFLC  C   L  RSF       YC  C++ KF+  C  C K    G     Y  + WH +C +C  C   +  + F        C+ C+    A KC  C   I   G    ++++   WH  CF C  C   L    F    ++  C  C
Sbjct:    5 FDCAKCSESLYGRKYIQTDDGP--YCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275          
BLAST of Four and A half lim domains vs. TrEMBL
Match: B1AAM6 (LIMPETin OS=Schistosoma mansoni OX=6183 PE=2 SV=1)

HSP 1 Score: 491.886 bits (1265), Expect = 1.807e-164
Identity = 260/572 (45.45%), Postives = 350/572 (61.19%), Query Frame = 3
Query:   96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQL-QDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIP--DCDRISS--EVQNHFDDKDED-KLIVQLARAFKKSRDTNYLGFGEIEPVDNQ--EVCSICDKQFEDEDIFCIRI--------------THSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  C+S C  +  H WR  C  C C  Y H   +D   +  S         +D L       L Q+Y  A ++A+   L WLPIG++Q E+  FL       P   L    +    R+  +  QIP  DC+   +  E+ NH  D + + +  ++ A  FK  R+++ LG G +E ++ +  + C+ C       D FCIRI              T +   A H++CF C TC E LVD+ Y W N  K ++CLRH+G+    RC  CD LI ++++T+AM+QE H  HFACH CD SLTG +YILRDD P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C  C  SL DR F TK+  LYC +C+DE+F++ CDGC  +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP   ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+  KFTSK+E+PYC +C+ ELF K+C KCT+PI+          GFGGCKFIS  D HWH+ECF C KC +++VG+GF   +D ++C  CG
Sbjct:   11 CLKCQSKCSGFMKHSWRMICTQCHCAYYEH--DIDYYYTNQSI--------LDELDYNQSILLYQEYMNAQNLAKQYGLNWLPIGVKQTEVDLFLNSL----PNEELPRGEVADYIRRQRLRKQIPLQDCNPAVTIEELWNHSKDSNPNLENELREANRFKNFRNSHDLGIGLVEHMNEKDGQPCTNCSNTIHF-DEFCIRIKPEHSLTEEISNVSTSNHTPAWHLNCFRCTTCNEYLVDYIYAWFN--KQLYCLRHYGQSIRPRCVTCDHLIFSEEYTRAMEQEHHTGHFACHSCDVSLTGQRYILRDDEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACTTSLADRPFATKEEQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFLCVECKQPIGAKSFIPRENQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCSKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEDRHWHSECFLCGKCNSNLVGRGFLTSDDMIMCSECG 555          

HSP 2 Score: 113.235 bits (282), Expect = 1.599e-22
Identity = 71/232 (30.60%), Postives = 113/232 (48.71%), Query Frame = 3
Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            WH  CF C  C++ LVD  +   +  LYC   + +     C  C  +  +  ++Y  A +Q H    F C  C+ S+  + +I  + +  C+ CYE K A  C +C E I      +++K   WH++CF C++C   L++  F +KEE+ YC++C+ E F  RC  C Q +      K   +G             WH ECF C +C+  +  K F  +E+Q+VC  C  A+
Sbjct:  219 WHLNCFRCTTCNEYLVDYIYAWFNKQLYCLRHYGQSIRPRCVTCDHLIFS--EEYTRAMEQEHHTGHFACHSCDVSLTGQRYILRDDEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACTTSLADRPFATKEEQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFLCVECKQPIGAKSFIPRENQVVCVPCYEAK 436          
BLAST of Four and A half lim domains vs. TrEMBL
Match: U6J6Y3 (Four and a half LIM domains protein 2 OS=Echinococcus granulosus OX=6210 GN=EGR_09187 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.053e-164
Identity = 254/569 (44.64%), Postives = 337/569 (59.23%), Query Frame = 3
Query:   96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITHSAEF----------ASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  C S C  +  H WR  C +C C   +H     S DS           ++  L +     L+ Y  A ++A  + L WLP+G+Q +E+  FL       P   +         R   I +Q+P  DR     +S+   N         L  +   A  F+ SR+    G G +E    +   VC+ C +Q    + FC+RI                A H  CF C  C E LVDF Y W N     +CLRH+G++   RCA CD LI ++++T+AMDQE H  HFAC  CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C  C+ SL DR F TKD+ LYC +C+DE+F++ CDGC  +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+  KFTSK+E+PYC +C+ +LF K+C KC +PI+          GFGGCKFIS  D HWH+ECF CSKC  ++VG+GF   EDQ++C  CG
Sbjct:   11 CLTCASECPGFKLHPWRATCTACHCSYDKHAVTDPSKDS-----------YLHELELSDTTVLKAYDVAQTVAREHGLHWLPVGIQSSEVEAFLTSL----PSSEIPRGEAYVEMRTRRIRHQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCG 551          

HSP 2 Score: 106.686 bits (265), Expect = 2.307e-20
Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3
Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            DS D S+    WH  CF C  C + LVD  +   +   YC   + +     C  C  +  +  ++Y  A  Q H    F C  C+ S+  + +I  + +  C+ CYE K A  C +C E I      +++K   WH++CF C++C   L++  F +K+++ YC++C+ E F  RC  C Q +      K   +G             WH ECF C +C+  +  K F  +++Q+VC  C  A+
Sbjct:  205 DSSDNSYVP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432          
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A068Y1Z9 (Four and A half lim domains OS=Echinococcus multilocularis OX=6211 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.314e-164
Identity = 254/569 (44.64%), Postives = 337/569 (59.23%), Query Frame = 3
Query:   96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITHSAEF----------ASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  C S C  +  H WR  C +C C   +H     S DS           ++  L +     L+ Y  A ++A  + L WLP+G+Q +E+  FL       P   +         R   I +Q+P  DR     +S+   N         L  +   A  F+ SR+    G G +E    +   VC+ C +Q    + FC+RI                A H  CF C  C E LVDF Y W N     +CLRH+G++   RCA CD LI ++++T+AMDQE H  HFAC  CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C  C+ SL DR F TKD+ LYC +C+DE+F++ CDGC  +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+  KFTSK+E+PYC +C+ +LF K+C KC +PI+          GFGGCKFIS  D HWH+ECF CSKC  ++VG+GF   EDQ++C  CG
Sbjct:   11 CLTCGSECPGFKLHPWRATCTACHCSYDKHAVTDPSKDS-----------YLHELELSDTTVLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLTSL----PSSEIPRGEAYVEMRTRRIRHQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCG 551          

HSP 2 Score: 106.686 bits (265), Expect = 2.349e-20
Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3
Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            DS D S+    WH  CF C  C + LVD  +   +   YC   + +     C  C  +  +  ++Y  A  Q H    F C  C+ S+  + +I  + +  C+ CYE K A  C +C E I      +++K   WH++CF C++C   L++  F +K+++ YC++C+ E F  RC  C Q +      K   +G             WH ECF C +C+  +  K F  +++Q+VC  C  A+
Sbjct:  205 DSSDNSYVP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432          
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3W1E0 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS3546 PE=4 SV=1)

HSP 1 Score: 489.96 bits (1260), Expect = 8.052e-164
Identity = 255/572 (44.58%), Postives = 341/572 (59.62%), Query Frame = 3
Query:   96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMN---IFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRIT----------HSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  C S C  +  H WR  C +C C   +H     S DS           ++  L +     L+ Y  A ++A  + L WLP+G+Q +E+  FL              IP  +   +M    I +Q+P  DR     +S+   N         L  +   A  F+ SR+    G G +E    +   VC+ C +Q    + FC+RI           +S   A H  CF C  C E LVDF Y W N     +CLRH+G++   RCA CD LI ++++T+AMDQE H  HFAC  CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C  C+ SL DR F TKD+ LYC +C+DE+F++ CDGC  +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KCSE+IR+ G+TYKG+PWHKECF CTSC K L+  KFTSK+E+PYC +C+ +LF K+C KC +PI+          GFGGCKFIS  D HWH+ECF CSKC  ++VG+GF   +DQ++C  CG
Sbjct:   11 CLTCGSECPGFKLHPWRATCTNCHCSYEKHAVTDPSKDS-----------YLHELEVSDTTLLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLT-------SLPSSEIPRGEAYAEMRFRRIRHQVPPQDRKPASSLSTAKLNLPPSNAPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFRE-FCVRIRPEHRLSDSSDNSYAPAWHPGCFRCSNCSEHLVDFVYAWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCNCNLVGRGFLTNDDQIMCSDCG 551          

HSP 2 Score: 107.071 bits (266), Expect = 1.748e-20
Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3
Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            DS D S+    WH  CF C  C + LVD  +   +   YC   + +     C  C  +  +  ++Y  A  Q H    F C  C+ S+  + +I  + +  C+ CYE K A  C +C E I      +++K   WH++CF C++C   L++  F +K+++ YC++C+ E F  RC  C Q +      K   +G             WH ECF C +C+  +  K F  +++Q+VC  C  A+
Sbjct:  205 DSSDNSYAP-AWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432          
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3X1E4 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7003 PE=4 SV=1)

HSP 1 Score: 488.034 bits (1255), Expect = 4.978e-163
Identity = 253/569 (44.46%), Postives = 338/569 (59.40%), Query Frame = 3
Query:   96 CKICKS-CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDR-----ISSEVQNHFDDKDEDKLIVQLARA--FKKSRDTNYLGFGEIEPVDNQE--VCSICDKQFEDEDIFCIRITH----------SAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  C S C  +  H WR  C  C C   +H     S DS           ++  L +     L+ Y  A ++A+ + L WLP+G+Q AE+  FL       P   +    +    R   I +Q+P  DR     +S+   N         L  +   A  F+ SR+    G G +E V  +   VC+ C +     + FC+R+            S   A H  CF C  C E LVD  Y W N     +CLRH+G++   RCA CD LI ++++T+AMDQE H  HFAC  CD SLTG +YILRD+ P C+ C+E +F NTC +C EKI CDSKDLSFKE HWHEKCF C  C+ SL DR F TKD+ LYC +C+DE+F++ CDGC  +F+ G++KYEY G+QWHE+CF+C +C Q IG KSFIP + ++ C+ CYE K+AQ+C KC+E+IR+ G+TYKG+PWHKECF CTSC K L+  KFTSK+E+PYC +C+ ELF K+C KC +PI+          GFGGCKFIS  D HWH+ECF CSKC  ++VG+GF   +DQ++C  CG
Sbjct:   11 CLTCGSECSGFKLHPWRATCTGCHCSYDKHAVTDPSKDS-----------YLHELELSDVTVLKAYDVAQAIAKEHGLHWLPVGIQSAEVEAFLTSL----PSSEIPRGEVYAEMRFRRIRHQVPPQDRKPASSLSTAKLNLPPSTAPANLEGESREATRFQNSRNRRDFGIGRVERVSAKATIVCAECSEHIGFRE-FCVRVRPEHRLSDSSDTSYAPAWHPGCFRCSNCSEHLVDLVYGWLNGKP--YCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCAEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCGKPIT----------GFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNDDQIMCSDCG 551          

HSP 2 Score: 107.842 bits (268), Expect = 8.691e-21
Identity = 71/243 (29.22%), Postives = 115/243 (47.33%), Query Frame = 3
Query: 1032 DSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDC-FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGAR 1751
            DS D S+    WH  CF C  C + LVD  +   +   YC   + +     C  C  +  +  ++Y  A  Q H    F C  C+ S+  + +I  + +  C+ CYE K A  C +C E I      +++K   WH++CF C++C   L++  F +K+++ YC++C+ E F  RC  C Q +      K   +G             WH ECF C +C+  +  K F  +++Q+VC  C  A+
Sbjct:  205 DSSDTSYAP-AWHPGCFRCSNCSEHLVDLVYGWLNGKPYCLRHYGQMIRPRCATCDHLIFS--EEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGC-QGVFKAGMRKYEYRG-----------QQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAK 432          
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:103031012])

HSP 1 Score: 285.034 bits (728), Expect = 2.524e-91
Identity = 134/285 (47.02%), Postives = 179/285 (62.81%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWHE+CF C  C  SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C  C + I   G+TY   PWHK+CFLCT C++ LS  +FTS+++  YC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 135.961 bits (341), Expect = 1.561e-35
Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y    ++   +C++ +  L    C EC + I  N       D+ +H E F C  C  SL    +  +D+   C +C+ N++ + CH+C + I   S+ +  K   WHE CF C  C   +  +SF  KDN  YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 105.145 bits (261), Expect = 1.073e-24
Identity = 67/220 (30.45%), Postives = 106/220 (48.18%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  CK  LVD  +  S   + + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D+N +CV C+E QF   C  C + I+  +  +++ +  WH+ CFLC  C   L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEECFHCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:103023824])

HSP 1 Score: 274.633 bits (701), Expect = 2.689e-87
Identity = 129/284 (45.42%), Postives = 180/284 (63.38%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
             E F C  C  SL G KYI  +DNP+C+ C+++ F NTC +C E I  D+++L +++ H+HE CF C  C  SL D  FT++D+ L C +C+  +FSS C  C K    G +K EY G  WHE CFIC  C Q IG+KSFIP      C+ CYENK A +C +C +++ K G+TY+ +PWHKECFLCT C+  L+   FTS+++ PYC +CF  L+ K+C+ C++PI+          GFGG K+IS  +  WH  CF CS+C  S+VG GFF + D+++C  C
Sbjct:    2 TERFDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPIT----------GFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275          

HSP 2 Score: 130.183 bits (326), Expect = 2.303e-33
Identity = 80/276 (28.99%), Postives = 131/276 (47.46%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  CKE L    Y  + D+   +C+  +  L    C EC ELI   D  +    D+ +H   F C  CD SL    +  +DD   C  C+ N+F + C  C + +   ++ L +    WHE CF+C+ C   +  +SF    +  YC  C++ KF+  C  C ++   G     Y  + WH++CF+C  C   +  + F   +    C+ C+ N +A+KC  CS+ I   G    I+++   WH+ CF C+ C   L  + F  + ++  C +C
Sbjct:    5 FDCDNCKESLYGRKYIQAEDNP--YCIPCYDSLFANTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKG--GVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275          

HSP 3 Score: 121.709 bits (304), Expect = 1.671e-30
Identity = 66/224 (29.46%), Postives = 109/224 (48.66%), Query Frame = 3
Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            F CD C +SL  R +   ++  YC  C+D  F++ CD C ++     ++  Y  + +HE CF C  C++S+  + F   +  + C  CY N+ + KC  C + +      + Y G  WH+ CF+C SC++ +    F   ++  YC  C+   F  RC +C Q         +L KG      ++  D+ WH ECF C+ C+  + G+ F  ++D   C  C G
Sbjct:    5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQ---------VLAKGG-----VTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFG 214          

HSP 4 Score: 102.064 bits (253), Expect = 1.273e-23
Identity = 62/223 (27.80%), Postives = 107/223 (47.98%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352
            H  CF C  C   L D  +   +D  A+ C   +      +C  CD+ ++    T+ ++     +H   F C+ C+  +    +I   D+ +CV C+EN+F   C +C + ++     +++++  WH++CFLC  C   L  + FT++D+  YC  CF   ++  C+ C K      G K   +  +QWH+ CF C+ C+ S+    F P   +I C  C  N
Sbjct:   62 HEHCFRCFRCDRSLADEPFTSQDD--ALLCNDCYCNEFSSKCVACDKTVMPG--TRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLA--KGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSN 278          
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121])

HSP 1 Score: 232.261 bits (591), Expect = 1.909e-71
Identity = 120/285 (42.11%), Postives = 166/285 (58.25%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+S+DLS+K+ HWH+ CF C  C+ SLVD+ F+TK+  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ C+E + A +C +C ++      T        +CFLCT C++ LS  +FTS+++ PYC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC             D ++C  CG
Sbjct:    2 AERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNST--------DCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPIS----------GLGGSKYISFEERQWHNDCFNCKKCSI-----------DDILCPDCG 257          

HSP 2 Score: 108.227 bits (269), Expect = 6.419e-26
Identity = 70/254 (27.56%), Postives = 118/254 (46.46%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV-NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSC 1457
            ++C  CKE L    Y    ++   +C++ +  L    C EC + I  N       D+ +H + F C  C+ SL    +  +++   C +C+ N++ + C++C + I   S+ +  K   WHE CF C  C   +  +SF  KDN  YC  CF+++F+  C  C K+                 DCF+C  C + +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C
Sbjct:    5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNS----------TDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKC 246          

HSP 3 Score: 79.7221 bits (195), Expect = 3.595e-16
Identity = 58/200 (29.00%), Postives = 90/200 (45.00%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE---FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCN 1283
            H  CF C  C   LVD  +  S  ++ + C   +      +C EC + I+    ++ M+ +   +H   F C  C   +    +I +D+  +CV CFE QF   C +C +              +    CFLC  C + L  + FT++D++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+
Sbjct:   62 HDDCFHCFKCNRSLVDKPF--STKNEELLCTECYSNEYSSKCYECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKK----------VHTVYNSTDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCS 247          
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707])

HSP 1 Score: 210.69 bits (535), Expect = 5.988e-63
Identity = 111/287 (38.68%), Postives = 154/287 (53.66%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDN-YLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F C  C  +L G KY+ +DD   CV+CFE    NTC +C   I  D+K+L+ K  HWHE CF C  C+  L + SF  +D+  + C  C   + S+ C GC K+   G K  EY  K WHE+CF C +C Q I + SF+       C  C+E K A+ C +C E I  +GITY+  PWH ECF+C +C+K L+  +FT+ E+  YC +C+     K+C  C  PI+          GFG G   ++  ++ WH  CFNC KC  S+  K F L  + + C  C 
Sbjct:    2 TERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPIT----------GFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCA 278          

HSP 2 Score: 120.553 bits (301), Expect = 4.970e-30
Identity = 85/282 (30.14%), Postives = 125/282 (44.33%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNP-FCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C  C++ L    Y   +D     C+R F +L    CAEC   I V+       ++ +H + F C  C   L    +  RDD    C KC   +    C  C + I    K++ +K   WHE+CF C  C   +   SF TK +  YC  C ++KF+  C  C + I   G+    Y  K WH +CF+C  C + +    F        C+ CY+   A+KC  C   I   G     + Y+ + WH+ CF C  C   L+  +F    E  YCT+C  +L
Sbjct:    5 FDCFYCRDNLQGKKYVNKDDKHV--CVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGI---TYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 281          

HSP 3 Score: 101.293 bits (251), Expect = 2.362e-23
Identity = 60/219 (27.40%), Postives = 96/219 (43.84%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE-FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKI---FRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C + L +  +   +D K + C +        RC  C ++I+  D       + +H E F C  C   +    ++ + D+ +C  C E +F   C +C E I+  +  +++++  WH +CF+C  C   L    FT  ++Y YC +C+    +  C GC      F  G     Y    WHE CF C  C+ S+  K F+     I C  C
Sbjct:   62 HEDCFRCAKCYKPLANESFAARDDGK-VMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPIT--ANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 277          

HSP 4 Score: 54.299 bits (129), Expect = 1.378e-7
Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 3
Query: 1434 ECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQED-QMVCGSCGGARNDDG 1763
            E F C  C+  L   K+ +K++K  C  CF +L    C +C +PI                K ++  + HWH +CF C+KC   +  + F  ++D +++CG C GAR D  
Sbjct:    3 ERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDA------------KELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKC-GAREDSA 100          
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707])

HSP 1 Score: 211.075 bits (536), Expect = 6.303e-63
Identity = 111/287 (38.68%), Postives = 154/287 (53.66%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDN-YLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFG-GCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E F C  C  +L G KY+ +DD   CV+CFE    NTC +C   I  D+K+L+ K  HWHE CF C  C+  L + SF  +D+  + C  C   + S+ C GC K+   G K  EY  K WHE+CF C +C Q I + SF+       C  C+E K A+ C +C E I  +GITY+  PWH ECF+C +C+K L+  +FT+ E+  YC +C+     K+C  C  PI+          GFG G   ++  ++ WH  CFNC KC  S+  K F L  + + C  C 
Sbjct:   18 TERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPIT----------GFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCA 294          

HSP 2 Score: 120.939 bits (302), Expect = 5.180e-30
Identity = 85/282 (30.14%), Postives = 125/282 (44.33%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNP-FCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG-----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIEL 1532
            F+C  C++ L    Y   +D     C+R F +L    CAEC   I V+       ++ +H + F C  C   L    +  RDD    C KC   +    C  C + I    K++ +K   WHE+CF C  C   +   SF TK +  YC  C ++KF+  C  C + I   G+    Y  K WH +CF+C  C + +    F        C+ CY+   A+KC  C   I   G     + Y+ + WH+ CF C  C   L+  +F    E  YCT+C  +L
Sbjct:   21 FDCFYCRDNLQGKKYVNKDDKHV--CVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGI---TYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 297          

HSP 3 Score: 101.679 bits (252), Expect = 2.333e-23
Identity = 60/219 (27.40%), Postives = 96/219 (43.84%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQE-FHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKI---FRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF C  C + L +  +   +D K + C +        RC  C ++I+  D       + +H E F C  C   +    ++ + D+ +C  C E +F   C +C E I+  +  +++++  WH +CF+C  C   L    FT  ++Y YC +C+    +  C GC      F  G     Y    WHE CF C  C+ S+  K F+     I C  C
Sbjct:   78 HEDCFRCAKCYKPLANESFAARDDGK-VMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPIT--ANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 293          

HSP 4 Score: 54.6842 bits (130), Expect = 1.219e-7
Identity = 37/127 (29.13%), Postives = 57/127 (44.88%), Query Frame = 3
Query: 1386 IIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQED-QMVCGSCGGARNDDG 1763
              R SG          E F C  C+  L   K+ +K++K  C  CF +L    C +C +PI                K ++  + HWH +CF C+KC   +  + F  ++D +++CG C GAR D  
Sbjct:    3 FYRHSGPRSYLSSTMTERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDA------------KELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKC-GAREDSA 116          
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3])

HSP 1 Score: 166.777 bits (421), Expect = 1.696e-48
Identity = 77/172 (44.77%), Postives = 105/172 (61.05%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR-TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG 1403
            E + CH C  SL G KY+LR++NP+CV+C+E  + + C  C + I CDSKDLS+K+ HWHE CF C  C  SL D++F+ +D  L C  C    +S+ C G  K+F   G +K E+ G  WHE CF CA C Q IG+KSF+P      C+ CYE + AQ C  C ++  + G
Sbjct:   11 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGG 182          

HSP 2 Score: 97.4413 bits (241), Expect = 1.221e-23
Identity = 46/164 (28.05%), Postives = 82/164 (50.00%), Query Frame = 3
Query: 1080 FLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG---ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQ 1562
            + C  C +SL+ + +  ++   YC  C++  ++  C+ C +      K   Y  + WHE CF CA C +S+  K+F   + K+ C  C+ N ++ KC   S++    G   + ++G  WH+ CF C  CQ+ +    F  +E+  YC  C+ + F + C  C +
Sbjct:   13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK 176          

HSP 3 Score: 76.6406 bits (187), Expect = 1.741e-16
Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 3
Query: 1263 FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            + C  C +S+  K ++       C+ CYE  +   C  C + I      ++YK   WH+ CF C +C++ L++  F++++EK  CTEC    +  +C   ++  S     K+  +G             WH  CF C++CQ  +  K F  +E    C  C
Sbjct:   13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRG-----------SSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 162          

HSP 4 Score: 73.1738 bits (178), Expect = 2.934e-15
Identity = 47/172 (27.33%), Postives = 80/172 (46.51%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKIS-CDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTG 1217
            ++C  CKE L+   Y    ++   +C+R +  L    C  C + I   +KD +   D+ +H   F C  C+ SL    +  RD+   C +C  N +   C   S+  S   ++ + F+   WHE CF C  C   +  +SF  ++   YC  C++++F+  C  C K+   G
Sbjct:   13 YDCHYCKESLLGKKYVLREENP--YCVRCYETLYTHACESCKQPIGCDSKDLS-YKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 181          

HSP 5 Score: 62.3882 bits (150), Expect = 1.818e-11
Identity = 34/104 (32.69%), Postives = 52/104 (50.00%), Query Frame = 3
Query: 1434 ECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGC--KFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            E + C  C++ L   K+  +EE PYC  C+  L+   C+ C QPI              GC  K +S  D HWH  CF C+ C+ S+  K F  ++++++C  C
Sbjct:   11 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPI--------------GCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTEC 100          
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3])

HSP 1 Score: 161.77 bits (408), Expect = 1.531e-46
Identity = 75/173 (43.35%), Postives = 102/173 (58.96%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGIT 1409
            E + CH C  SL G KY+LR++NP+CV+C+E  + + C  C + I CDSKDLS+K+ HWHE CF C  C  SL D++F+ +D  L C  C    +S+ C         G +K E+ G  WHE CF CA C Q IG+KSF+P      C+ CYE + AQ C  C ++  + G T
Sbjct:    4 ERYDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCA--ANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPT 174          

HSP 2 Score: 97.8265 bits (242), Expect = 9.017e-24
Identity = 46/174 (26.44%), Postives = 82/174 (47.13%), Query Frame = 3
Query: 1086 CDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGF 1607
            C  C +SL+ + +  ++   YC  C++  ++  C+ C +      K   Y  + WHE CF CA C +S+  K+F   + K+ C  C+ N ++ KC      +    + ++G  WH+ CF C  CQ+ +    F  +E+  YC  C+ + F + C  C +   +    +L    F
Sbjct:    8 CHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPTRLSPAPF 181          

HSP 3 Score: 75.8702 bits (185), Expect = 4.024e-16
Identity = 42/161 (26.09%), Postives = 71/161 (44.10%), Query Frame = 3
Query: 1263 FICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIR--KSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            + C  C +S+  K ++       C+ CYE  +   C  C + I      ++YK   WH+ CF C +C++ L++  F++++EK  CTEC    +  +C     P+              G + +      WH  CF C++CQ  +  K F  +E    C  C
Sbjct:    6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPV--------------GTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 152          

HSP 4 Score: 72.7886 bits (177), Expect = 5.414e-15
Identity = 45/171 (26.32%), Postives = 78/171 (45.61%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIV--NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTG 1217
            ++C  CKE L+   Y    ++   +C+R +  L    C  C + I   +KD +   D+ +H   F C  C+ SL    +  RD+   C +C  N +   C      +   ++ + F+   WHE CF C  C   +  +SF  ++   YC  C++++F+  C  C K+   G
Sbjct:    6 YDCHYCKESLLGKKYVLREENP--YCVRCYETLYTHACESCKQPIGCDSKDLS-YKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVG--TRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 171          
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl3b (four and a half LIM domains 3b [Source:ZFIN;Acc:ZDB-GENE-030131-8356])

HSP 1 Score: 161.384 bits (407), Expect = 6.549e-46
Identity = 83/237 (35.02%), Postives = 116/237 (48.95%), Query Frame = 3
Query: 1044 LSFKEWHWHEKCFLCDLCHDSL-VDRSFTTKDNYLYCPNCFDEK---FSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            LS    HWHE CF C  C   +     +  +   + C  C           C  C +  R GV K  Y G+ WHE CF CA C ++IGTKSF+P   +I C+ C E K A +CF C + + + G+ YK   +H+EC +C  C   L+     ++ E  YC  CF + + K+C  C +PI+             G KF++     WHTECF C +C  +M GKGF  + D + CG C 
Sbjct:    2 LSLDNLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSA----------AGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCA 228          

HSP 2 Score: 90.5077 bits (223), Expect = 7.612e-21
Identity = 56/221 (25.34%), Postives = 100/221 (45.25%), Query Frame = 3
Query:  882 FHVEHFACHVCDSSL-TGTKYILRDDNPFCVKCFENQ---FGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            +H   F C  C + +   T+Y+L      C  C  +     G++C  C   I      +++K   WHE+CF C  C +++  +SF    + +YC +C ++K ++ C  C K      K   Y    +H +C +C  CN  +   +       + C  C+E K+++KC  C + I  +     +T+    WH ECF C  C   ++   F ++ +  +C +C
Sbjct:    9 WHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTE--KGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDC 227          

HSP 3 Score: 63.929 bits (154), Expect = 1.076e-11
Identity = 39/159 (24.53%), Postives = 69/159 (43.40%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKI--SCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDE 1169
            H  CF C  C E +    +    D   I+C+    + T  RC  C + +  K      +  +H E   C  C++ L G     R +  +C  CFE ++   C  C + I  +  +K L+     WH +CF C  C  ++  + F  + + ++C +C ++
Sbjct:   75 HERCFACARCMETIGTKSFVPHGDE--IYCVSCNEKKTATRCFTCHKPVTEKGVVYK-EHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQ 230          
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718])

HSP 1 Score: 109.383 bits (272), Expect = 1.872e-27
Identity = 64/226 (28.32%), Postives = 101/226 (44.69%), Query Frame = 3
Query:  861 TKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535
              AM + +H EHF C  C   +    +  +D  P+C + + + F   C  C+E +  + + ++  +  WH + F C  C     +  F  +D   YC   +   F+  C+GC    R  ++ Y  A    WH +CFIC +C       SF    G+  C   Y  +    C  C + I    I+     +H E F+C  C K L++  F  + +KPYC  CFI+LF
Sbjct:    3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCS---RAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLF 225          

HSP 2 Score: 90.8929 bits (224), Expect = 6.046e-21
Identity = 64/225 (28.44%), Postives = 89/225 (39.56%), Query Frame = 3
Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            WH + F+C  C   +  R+F  KD   YC   +   FS  C  C +      +      + WH + F CA C +  G + F   +GK  C   Y    A KC  CS  I ++ ++     WH ECF+C  C        F   E +PYC   + E     C  C +PI+            G C  IS     +H E F C+ C   +    F  Q D+  C  C
Sbjct:   10 WHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPIT------------GRC--ISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPC 220          

HSP 3 Score: 83.9593 bits (206), Expect = 1.358e-18
Identity = 56/189 (29.63%), Postives = 86/189 (45.50%), Query Frame = 3
Query:  783 FCLRHFGELTYCRCAECDELIV-NKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCY 1346
            +C   +  L   RCA C+E ++ N+    A+D+ +H EHF C  C        +  RD  P+C + +   F   C+ CS  I      LS     WH +CF+C  C    ++ SF   +   YC   + E+  S C GC K   TG +      K++H + F+CA C + +   +F   N K  C  C+
Sbjct:   37 YCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIM--QNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPI-TG-RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCF 221          

HSP 4 Score: 58.9214 bits (141), Expect = 5.054e-10
Identity = 31/109 (28.44%), Postives = 49/109 (44.95%), Query Frame = 3
Query: 1404 ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730
            +T  G  WH E F+C  CQ  +    F  K+ +PYC E +  LF  RC  C + + D +            + ++  D  WH E F C++C      +GF  ++ +  C
Sbjct:    3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQ------------RVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYC 99          

HSP 5 Score: 46.9802 bits (110), Expect = 4.014e-6
Identity = 41/192 (21.35%), Postives = 77/192 (40.10%), Query Frame = 3
Query:  603 VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFS 1178
            +DNQ V +  D+ +  E  FC +      F                  FH     D K  +C + +  +   +C  C   I+ +++  A++  +H E F C  C +      +   +  P+C   +  + G+ C  C + I+   + +S     +H + F+C  C   L   +F  +++  YC  CF + F 
Sbjct:   58 MDNQRVVTALDRTWHPEHFFCAQC--GRPFGEE--------------GFH---ERDGKP-YCRQDYYGMFAPKCNGCSRAIM-QNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPIT--GRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLFG 226          
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718])

HSP 1 Score: 114.39 bits (285), Expect = 4.691e-27
Identity = 77/244 (31.56%), Postives = 107/244 (43.85%), Query Frame = 3
Query:  807 LTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535
            L   +CA C + I  +  T AM + +H EHF C  C   +    +  RD  P+C K +   F   C  CS  I    K ++  +  WH + F C  C     D  F  KD   YC   + E F+  C GC +     V+ Y  A    WH +CF+C +C       SF    G+  C   Y  +    C  C + I    IT     +H E F+C  C K L++  F  + +KPYC  CFI+LF
Sbjct:  317 LAKGQCAACLKPIAGQVVT-AMGRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPIL--DKVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAI---VENYISALSALWHPECFVCRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFIKLF 554          

HSP 2 Score: 89.3521 bits (220), Expect = 5.082e-19
Identity = 48/164 (29.27%), Postives = 71/164 (43.29%), Query Frame = 3
Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730
            G+ WH + F+C +C + IG ++F   +G   C   Y    + +C  CS  I    +T     WH E F C  C     +  F  K+ KPYC + + E+F  +C  C++ I +               +IS     WH ECF C +C    +   FF  E Q  C
Sbjct:  338 GRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILDKVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAIVEN--------------YISALSALWHPECFVCRECFTPFINGSFFEHEGQPYC 487          

HSP 3 Score: 82.8037 bits (203), Expect = 6.178e-17
Identity = 64/226 (28.32%), Postives = 88/226 (38.94%), Query Frame = 3
Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            WH + F+C  C + +  R+F  +D   YC   +   FS  C  C G I    V   +   K WH + F C  C    G + F   +GK  C   Y    A KC  CS  I ++ I+     WH ECF+C  C        F   E +PYC   + E     C  C +PI+      +  K              +H E F C+ C   +    F  Q D+  C  C
Sbjct:  341 WHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILDKVVTALD---KTWHPEHFFCVQCGHFFGDEGFHEKDGKPYCRQDYFEMFAPKCGGCSRAIVENYISALSALWHPECFVCRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKK--------------FHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 549          

HSP 4 Score: 62.003 bits (149), Expect = 2.306e-10
Identity = 36/122 (29.51%), Postives = 54/122 (44.26%), Query Frame = 3
Query: 1365 KCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730
            +C  C + I    +T  G  WH E F+CT CQ+ +    F  ++  PYC + +  LF  RC  C+ PI D              K ++  D  WH E F C +C      +GF  ++ +  C
Sbjct:  321 QCAACLKPIAGQVVTAMGRTWHPEHFVCTECQEEIGARNFFERDGLPYCEKDYHFLFSPRCAYCSGPILD--------------KVVTALDKTWHPEHFFCVQCGHFFGDEGFHEKDGKPYC 428          

HSP 5 Score: 48.521 bits (114), Expect = 4.208e-6
Identity = 29/99 (29.29%), Postives = 48/99 (48.48%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFG 995
            H  CF C+ C    ++  + + ++ +  +C  H+ E     C+ C + I  +  T AM ++FH EHF C  C   L    +  ++D P+C  CF   FG
Sbjct:  460 HPECFVCRECFTPFINGSF-FEHEGQP-YCEVHYHERRGSLCSGCQKPITGRCIT-AMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFIKLFG 555          
BLAST of Four and A half lim domains vs. Ensembl Yeast
Match: LRG1 (GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains [Source:SGD;Acc:S000002399])

HSP 1 Score: 49.2914 bits (116), Expect = 1.894e-6
Identity = 47/151 (31.13%), Postives = 60/151 (39.74%), Query Frame = 3
Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKIS-----CIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDH-WHTECFNCSK 1673
            GK +HE CF C DC + +  K F     K S     C   Y  +H   C  C   +R    T  G  + +E F CT C       K      + YC   F++ F KRCK C  PISD              +F    + H WH EC+   K
Sbjct:   50 GKYYHESCFTCQDCQKPLKPKYFPYQVDKTSESILLCQYDYFRRHNLLCHVCDTPLRGLYYTAFGYRYDEEHFSCTICATPCGVKKCFMYGNQLYCKYHFLKYFSKRCKGCEFPISDQYI-----------EFPKGEEIHCWHPECYGIHK 189          
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41100 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D2])

HSP 1 Score: 290.426 bits (742), Expect = 1.310e-90
Identity = 169/547 (30.90%), Postives = 277/547 (50.64%), Query Frame = 3
Query:  114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGP-GFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIE-PVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKE-WHWHEKCFLCDLCHDSL-VDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  ++ H W+  C  C+CP   H              Q+  N  +D  T  +   L      IS      L W P GL Q+++  +++   +  P    +++ P ++  R   + YQ+P  DR   E  + F     +  +      F  S D N  G G ++ P   ++ C+ C       +   +     AE + H  CF C+TC E LV+  Y    D   ++C RHF EL   RC  CDELI   ++T AM++ +H+ HF C  CD S+TG ++I+R D P C  CF++ + + C  C +KI  +S+D+S  +  HWH+KCF+CD+C   L  + S  + +    C  C+   +   C  CG+I  +G  + EY+G  WHE+CF CA+C ++IGT  F+P +    C GCY++K +++C  C E + + G+ Y G+ WHK CF C  C + L+ + F+ ++   YC EC+ + + K+C+ C             +K   G ++ ++ + ++H ECF CS+C  S+  +GF  + D+++CG C 
Sbjct:   20 CPGFSLHVWKKACQYCKCPREDH------------DIQNEANETMDINTRINGIDLPPPPKVISGGFPKYL-WTPSGLSQSQVELYMK---ELPPQKVPIKDSPGSKYYR-AQMAYQLPVQDR-DPEKLSTFSAAHRESFM-----QFNSSAD-NARGEGVVKVPFGRRQRCARCSHPILRSEPAVVAENIGAEASFHPGCFTCETCNELLVELTYFQHADK--VYCGRHFAELQKSRCGGCDELIFTGEYTVAMNKNWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQKIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEIC-------------LKAIVGGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCA 527          

HSP 2 Score: 117.857 bits (294), Expect = 5.406e-28
Identity = 81/320 (25.31%), Postives = 134/320 (41.88%), Query Frame = 3
Query:  819 RCAECDELIVNKDFTK-----AMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAG---KQWHEDCFICADCNQSIGTK-SFIPINGKISCIGCYENKHAQKCFKCSEII--RKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            RCA C   I+  +          +  FH   F C  C+  L    Y    D  +C + F     + C  C E I      ++  + +WH   F C  C  S+  R F  + +   C +CF + ++  C+ C +  + G +  + +    + WH+ CFIC  C + + ++ S      K  C  CY   + ++C  C +II    S + Y G+ WH+ CF C +C + +  S F  K++  +C  C+   F KRC  C +P+ +   L                 + WH  CF+C  C  S+    F +++    C  C G
Sbjct:  166 RCARCSHPILRSEPAVVAENIGAEASFHPGCFTCETCNELLVELTYFQHADKVYCGRHFAELQKSRCGGCDELIFTGEYTVAMNK-NWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQ--KIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVL--------------YNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYG 468          

HSP 3 Score: 63.929 bits (154), Expect = 9.259e-11
Identity = 43/166 (25.90%), Postives = 76/166 (45.78%), Query Frame = 3
Query:  669 RITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFD 1166
            R+ +S  F  H +CF C  C E +    +   +D+   FC   +      RCA C E ++         + +H   F+C+ C  SL    + +RD   +C++C+   +   C  C + I    +  + +E ++H++CF+C  C  SL    F  + + L C +C D
Sbjct:  368 RLEYSGNFW-HENCFRCANCGEAIGTSGFVPKDDT--FFCPGCYQSKFSKRCASCGEPLLEGGVLYN-GETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAIV-GGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCAD 528          
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: LIMPET (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX6])

HSP 1 Score: 273.863 bits (699), Expect = 4.655e-84
Identity = 169/550 (30.73%), Postives = 253/550 (46.00%), Query Frame = 3
Query:  114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIP----INQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARA---FKKSRDTNYLGFGEIEPVDNQE-VCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            C  ++ H WR  C SC+CP+  HV   ++ D  +   +  V      +   SH         +  A      W+P GL   EI  ++ +  +       + IP    +    R   +  QIP  D         F  K   KL +    A   F + R    LG G +     Q   C  C+      D+         +   H+ CF C+   E LVD  YC  +D   I+C RH+GE    RCA C+ELI   ++++A+++ +H  H  C  CD SL+  K++  + +P C +C++  F N C  C E I   SKD+  +  HWHE CF C  C   L++  FT KD  L C  C     S  C  C   F  G KK  Y  K +H+ CFIC +C Q IG+K FI  + +  C  C+++K A+ C KC+++I+ S + + G  +H ECF C  C K L+ S FT +E +  C  C+ E + KRC  C   I             G  KF++  + ++H ECF C KC   + G+ F +++ + +C  C
Sbjct:   41 CRGFSVHPWRKFCNSCKCPHELHVLSTNNNDQNTPEERMGVEEEDPPMLQGSH---------LQQAMMEGYEWVPRGLTSEEIQIYMSLLPE-------DKIPKLKSLGAQHRTKQLSLQIPLQD---------FSSKHCKKLTLDQKMAMDDFCEKRIKTALGVGAVRACMAQPSTCKRCNGPVNPGDMALFAERLGPDSCWHVKCFTCEEDGELLVDLIYCSKDDE--IYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGPGSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRGINPSKVCAACNGDFAPGEKKVGYQSKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSSVQHAGSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIE------------GNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPC 551          

HSP 2 Score: 81.6481 bits (200), Expect = 2.859e-16
Identity = 48/167 (28.74%), Postives = 76/167 (45.51%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            WH  CF C +  + +    +   + +I C   +  K   +C  C E+I     +   +  WH     C+ C + LS  KF + E  P C  C+ E F  RC+ C +PI              G K + V   HWH  CF CS+C   ++ +GF L++++++C  C G
Sbjct:  218 WHVKCFTCEEDGELLVDLIYCSKDDEIYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGP------------GSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRG 372          

HSP 3 Score: 65.0846 bits (157), Expect = 5.258e-11
Identity = 43/189 (22.75%), Postives = 83/189 (43.92%), Query Frame = 3
Query:  603 VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDE 1169
            ++  +VC+ C+  F   +    ++ + ++   H  CF C  CK+ +    +   ++ +   C   F       C +C+++I       A    +H E F CH CD  L G+ +  ++    C  C+  ++   C  C   I  ++K +++ E ++H +CF C  C+  L    F  +D    C  C D+
Sbjct:  373 INPSKVCAACNGDFAPGEK---KVGYQSK-TFHDKCFICDECKQPIGSKQFIRRDERR--LCNNCFDSKFAKVCVKCNQVIKTSSVQHA-GSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIEGNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPCDDD 554          
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49798 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX7])

HSP 1 Score: 142.51 bits (358), Expect = 1.990e-37
Identity = 106/365 (29.04%), Postives = 162/365 (44.38%), Query Frame = 3
Query:  111 SCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIP----INQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFAS--------HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWH--EKCFLCDLCHDSLVDRSFTTKDNYLYC-PNC 1160
            +C  ++ H WR  C +C+CP   H    D   +G         N   +    + +   D  +  ++ E     W+P GL   ++  ++    +       E +P    I +  R   I  Q+P  D   +   N   ++++ +L       F   R  + LG G ++ V        C +  E     C+     A +AS        H SCF C TCKE LVD  Y +  DSK ++C RH  E    RCA CDE+I  +  T+A D  +HV+HF C  CD  L G +Y++RD+ P+C  CFE+ +   C  C E I  D+  ++    HWH   +CF C  C  +L+   F  K   +YC P+C
Sbjct:   19 TCPGFSAHYWRKICRNCKCPREDHNIISDDARAG--------RNIYMADVHRASSLASDDDSGCALDE---YAWVPPGLGPEQVHSYMNALPE-------EKVPYVDSIGERYRNRQIILQLPPHDN-EARFCNGLSEEEKRELCF-----FVALRKRDSLGRGVVKQVPEMSEGYSCKECGE-----CVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIY-FYKDSK-VYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCSPDC 352          

HSP 2 Score: 73.1738 bits (178), Expect = 5.991e-14
Identity = 56/186 (30.11%), Postives = 83/186 (44.62%), Query Frame = 3
Query:  981 ENQFGNTCHKCSEKISCDSKDL--SFKEWH--WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRK--SGITYKGDPWHK--ECFLCTSCQKMLSESKFTSKEEKPYCT 1514
            E   G +C +C E ++  S  +  S    H  WH  CF+C  C + LVD  +  KD+ +YC     E     C  C +I     +  E     WH   F C +C+  +G   ++  + K  C  C+E+ +A+ C  C E I    S + + G  WH   ECF C +C K L    F  K  + YC+
Sbjct:  165 EMSEGYSCKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEII-FAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349          

HSP 3 Score: 72.0182 bits (175), Expect = 1.664e-13
Identity = 51/189 (26.98%), Postives = 78/189 (41.27%), Query Frame = 3
Query: 1185 CDGCGKIFRTG-VKKYEYAGKQ---WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHT--ECFNCSKCQASMVGKGFFLQEDQMVC 1730
            C  CG+    G +  Y     Q   WH  CFIC  C + +    +   + K+ C   +      +C  C EII     T   D  WH + F C  C   L   ++  ++ KPYC  CF  L+ + C  C +PI +    ++   G            HWH   ECF+C  C  +++G  F  +  ++ C
Sbjct:  172 CKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPI-EPDASQMAHNG-----------QHWHATNECFSCCTCGKALLGLPFLPKSGEIYC 348          
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S548])

HSP 1 Score: 123.635 bits (309), Expect = 9.084e-32
Identity = 75/241 (31.12%), Postives = 116/241 (48.13%), Query Frame = 3
Query:  822 CAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKI--SCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYA-GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELF 1535
            CA C++ I+ +  T A+ + +H EHFAC  C++ L    +  RD  P+C + + + F   C  C+  I  SC    ++  +  WH + F+C  C +   D  F  +D   +C   +   F+  C GCG+     +  Y  A    WH +CFIC +C Q     SF   +G+  C   Y  K    C+ C + I    IT     +H E F+C  C K L++  F  + +KPYC  CF++LF
Sbjct:   26 CAACNKPIIGQVCT-ALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSC----VTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI---MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITGRCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLF 258          

HSP 2 Score: 101.293 bits (251), Expect = 5.490e-24
Identity = 53/164 (32.32%), Postives = 76/164 (46.34%), Query Frame = 3
Query: 1239 GKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVC 1730
            GK WH + F C  C   +GT++F   +GK  C   Y +  A +C  C+  I  S +T     WH E F+C  C     ++ F  ++ KP+C E +  +F  RC  C QPI D               +IS    HWH ECF C++C+    G  FF  + +  C
Sbjct:   42 GKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDN--------------YISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYC 191          

HSP 3 Score: 84.7297 bits (208), Expect = 2.376e-18
Identity = 62/226 (27.43%), Postives = 93/226 (41.15%), Query Frame = 3
Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            WH + F C  C   L  ++F  +D   YC   + + F+  C  C G I  + V   +   + WH + F+CA+C    G   F   +GK  C   Y    A +C  C + I  + I+     WH ECF+CT C++      F   + +PYC   +       C  C +PI+            G C  I+     +H E F C+ C   +    F  Q D+  C  C
Sbjct:   45 WHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALD---QTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPIT------------GRC--ITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 253          

HSP 4 Score: 48.1358 bits (113), Expect = 4.393e-6
Identity = 29/99 (29.29%), Postives = 45/99 (45.45%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFG 995
            H  CF C  C++      + + +D +  +C  H+       C  C + I  +  T AM ++FH EHF C  C   L    +  ++D P+C  CF   FG
Sbjct:  164 HAECFICTECRQPFPGGSF-FDHDGRP-YCEMHYHAKRGTLCYSCQKPITGRCIT-AMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259          
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49801 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY0])

HSP 1 Score: 122.479 bits (306), Expect = 3.254e-30
Identity = 92/362 (25.41%), Postives = 154/362 (42.54%), Query Frame = 3
Query:  114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESN-------NLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLD----RKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEP---VDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWH--EKCFLCDLCHDSLVDRSFTTKDNYLYC 1151
            C+    H WR  C +C+C    H  + D  +      +H V + +   +  +H  L+DY   ++ A             W+P G+ Q  +  ++            E +PI   +    R+  + YQ+P  D   +   N   D +  K+       F   R+ + LG G+I       ++  C  C K     ++         +   H  CF C  C   LVD  Y + +    I+C RH+ E    RCA CDELI ++ +T+A D+ +H  HF C  CD  L G  Y+ R   P C++C++  +   C  C + I+ D+K +  +   WH   +CF C  C+  ++ + F    N ++C
Sbjct:   38 CEGLDLHFWRKICKNCKCKQEDHDLYEDIAN------EHEVKSRLFKDSNVAH--LRDYFNKLTAANDQPKTEFVKKFTWVPPGMNQGILVKYME-------SLPKELVPIVGSEGAKYRRAQMVYQLPIHDHDPNYCDN-LSDAERQKM-----EDFCAMRNQDALGVGDIREKTQAASKWNCFRCSKPVMTGEVAVFASRAGEDKCWHPGCFVCTVCNNLLVDLIYFYKDG--VIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFC 376          

HSP 2 Score: 83.5741 bits (205), Expect = 3.256e-17
Identity = 46/155 (29.68%), Postives = 78/155 (50.32%), Query Frame = 3
Query: 1065 WHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGK-IFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG--ITYKGDPWHK--ECFLCTSCQKMLSESKFTSKEEKPYCT 1514
            WH  CF+C +C++ LVD  +  KD  +YC   + E+F   C  C + IF     + E   + WH+  F C +C++ +G + ++   G+  C+ CY+  +A+ C  C + I      I ++G  WH   ECF C  C K +   +F   +   +C+
Sbjct:  225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAE--DRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCS 377          

HSP 3 Score: 77.0258 bits (188), Expect = 4.273e-15
Identity = 46/164 (28.05%), Postives = 72/164 (43.90%), Query Frame = 3
Query: 1248 WHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDP-WHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWH--TECFNCSKCQASMVGKGFFLQEDQMVC 1730
            WH  CF+C  CN  +    +   +G I C   Y  +   +C  C E+I     T   D  WH+  F C  C + L    + ++  +P+C EC+ + + K C  C + I+               K I      WH  +ECF+C+KC   M+GK F   ++ + C
Sbjct:  225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIA------------ADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFC 376          
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:101169645])

HSP 1 Score: 286.189 bits (731), Expect = 7.024e-92
Identity = 135/285 (47.37%), Postives = 180/285 (63.16%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
             E + CH C  SL G KY+LR++NP+CVKC+E+ + NTC +C + I C+++DLS+K+ HWHE CF C  C  SLVD+ F+TKD  L C  C+  ++SS C  C K    G +K E+ G  WHE CF C  C Q IGTKSFIP +    C+ CYE + A +C  C + I   G+TY+  PWHK+CFLCTSC++ LS  +FTSK++  YC  CF  L+ K+C  CT PIS          G GG K+IS  +  WH +CFNC KC  S+VG+GF  + D ++C  CG
Sbjct:    2 TERYDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPIS----------GLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECG 276          

HSP 2 Score: 137.117 bits (344), Expect = 7.266e-36
Identity = 82/275 (29.82%), Postives = 132/275 (48.00%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            ++C  CKE L    Y    ++   +C++ +  L    C EC + I  N       D+ +H + F C  C  SL    +  +D+   C +C+ N++ + CH+C + I   S+ +  K   WHE CF C  C   +  +SF  KDN+ YC  C++++F+  C  C K   TG     Y  + WH+DCF+C  C Q +  + F   +    C+ C+ N +A+KC  C+  I   G    I+++   WH +CF C  C   L    F ++ +   C EC
Sbjct:    5 YDCHYCKESLFGKKYVLREENP--YCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          

HSP 3 Score: 107.457 bits (267), Expect = 1.584e-25
Identity = 68/220 (30.91%), Postives = 108/220 (49.09%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH---FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGC-GKIFRTGVKKY-EYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CF+C  CK  LVD  +  S   + + C   +      +C EC + I+    ++ M+ + H  H   F C  C   +    +I +D++ +CV C+E QF   C  C + I+  +  +++++  WH+ CFLC  C   L  + FT+KD++ YC NCF   ++  C  C   I   G  KY  +  +QWH DCF C  C+ S+  + F+     I C  C
Sbjct:   62 HEDCFKCFQCKRSLVDKPF--STKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPIT--TGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275          
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl5 (four and a half LIM domains protein 2-like [Source:NCBI gene;Acc:101156370])

HSP 1 Score: 278.87 bits (712), Expect = 6.656e-89
Identity = 130/284 (45.77%), Postives = 174/284 (61.27%), Query Frame = 3
Query:  891 EHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            E F CH C  SL G KYI+++D  +C KC+EN F N C +CS  I C+SKDLS+K+ HWHE+CF C  C  SL D++F  +++ L C  C+   +SS C  C K    G +K EY G  WHE CF+C  C Q +GTKSFIP      C+ C+E + A +C  C + I   G+TY+  PWH+ECFLC  C++ LS  +FTS+E  PYC ECF  L+ K+C  CT+PI+             G K+IS  +  WH+ECFNC +C  S+VG+GF  Q D ++C  CG
Sbjct:    5 ERFDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITS----------LAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECG 278          

HSP 2 Score: 134.42 bits (337), Expect = 6.982e-35
Identity = 84/278 (30.22%), Postives = 130/278 (46.76%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIE 1529
            F+C  CK+ L+   Y    D++  +C + +  L    C EC   I  N       D+ +H + F C  C  SL    +  R+D   C +C+   + + C  C + I   S+ + +K   WHE CFLC  C   L  +SF  K+N  +C  CF+++F+  C  C K   TG   Y+   K WH +CF+C  C + +  + F        C+ C+ N +A+KC  C++ I        I+++   WH ECF C  C   L    F ++ +   CTEC  E
Sbjct:    7 FDCHYCKDSLLGKKYIMKEDTQ--YCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQ--DKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGRE 280          

HSP 3 Score: 107.071 bits (266), Expect = 2.609e-25
Identity = 66/224 (29.46%), Postives = 104/224 (46.43%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENK 1355
            H  CF+C  C   L D  +    D   + C   +      +C  C + I+    ++ M+     +H   F CH C   L    +I +++  FCV CFE QF   C  C + I+  +  +++++  WH +CFLC  C   L  + FT+++NY YC  CF   ++  C GC K   +  G K   +  +QWH +CF C  C+ S+  + F+     I C  C   K
Sbjct:   64 HEQCFKCAKCSRSLADKAFAARED--LLLCTECYALDYSSKCITCKKTILPG--SRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAIT--TGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGREK 281          
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533])

HSP 1 Score: 277.715 bits (709), Expect = 8.221e-88
Identity = 132/293 (45.05%), Postives = 186/293 (63.48%), Query Frame = 3
Query:  861 TKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
            ++A+ +    + F C  C  SL G KYI  +D+P+C+ C+++ F NTC +C E I  D+++L +++ H+HE CF C  C  SL D  FT++DN L C +C+  +FSS C  C KI   G +K EYAG  WHE CFIC  C Q IG+KSFIP   +  C+ CYE+K A +C +C + + K G+TY+ +PWHKECF+CTSC+  L+   FTS++E PYC +CF  L+ K+C+ C +PI+          GFGG K+IS  D  WH  CF CS+C  S+VG GFF   D+++C  C
Sbjct:   40 SQALKKTTMADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPIT----------GFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322          

HSP 2 Score: 129.798 bits (325), Expect = 8.187e-33
Identity = 81/276 (29.35%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  CKE L    Y  SNDS   +C+  +  L    C EC ELI   D  +    D+ +H   F C  CD SL    +  +D+   C  C+ N+F + C  C + +   ++ L +    WHE CF+C  C   +  +SF    +  YC  C+++KF+  C  C K    G     Y  + WH++CF+C  C   +  + F   +    C+ C+ + +A+KC  C + I   G    I+++   WH+ CF C+ C   L  + F    ++  C +C
Sbjct:   52 FDCDNCKESLYGRKYIQSNDSP--YCIPCYDSLFSNTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322          

HSP 3 Score: 104.76 bits (260), Expect = 3.783e-24
Identity = 61/223 (27.35%), Postives = 105/223 (47.09%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH---FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352
            H  CF C  C   L D  +  ++   A+ C   +      +C  CD++++    T+ ++      H   F CH C+  +    +I   D  +CV C+E++F   C +C + +S     +++++  WH++CF+C  C   L  + FT++D   YC  CF   ++  C+ C K      G K   +  +QWH+ CF C+ C+ S+    F P   +I C  C  N
Sbjct:  109 HEHCFRCFRCDRSLADEPF--TSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 325          
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533])

HSP 1 Score: 273.478 bits (698), Expect = 7.685e-87
Identity = 131/284 (46.13%), Postives = 181/284 (63.73%), Query Frame = 3
Query:  888 VEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC 1739
             + F C  C  SL G KYI  +D+P+C+ C+++ F NTC +C E I  D+++L +++ H+HE CF C  C  SL D  FT++DN L C +C+  +FSS C  C KI   G +K EYAG  WHE CFIC  C Q IG+KSFIP   +  C+ CYE+K A +C +C + + K G+TY+ +PWHKECF+CTSC+  L+   FTS++E PYC +CF  L+ K+C+ C +PI+          GFGG K+IS  D  WH  CF CS+C  S+VG GFF   D+++C  C
Sbjct:    2 ADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPIT----------GFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275          

HSP 2 Score: 129.028 bits (323), Expect = 5.567e-33
Identity = 81/276 (29.35%), Postives = 129/276 (46.74%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAM--DQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C  CKE L    Y  SNDS   +C+  +  L    C EC ELI   D  +    D+ +H   F C  CD SL    +  +D+   C  C+ N+F + C  C + +   ++ L +    WHE CF+C  C   +  +SF    +  YC  C+++KF+  C  C K    G     Y  + WH++CF+C  C   +  + F   +    C+ C+ + +A+KC  C + I   G    I+++   WH+ CF C+ C   L  + F    ++  C +C
Sbjct:    5 FDCDNCKESLYGRKYIQSNDSP--YCIPCYDSLFSNTCDECKELI-GHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275          

HSP 3 Score: 103.605 bits (257), Expect = 3.187e-24
Identity = 61/223 (27.35%), Postives = 106/223 (47.53%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMD---QEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352
            H  CF C  C   L D  +  ++   A+ C   +      +C  CD++++    T+ ++     +H   F CH C+  +    +I   D  +CV C+E++F   C +C + +S     +++++  WH++CF+C  C   L  + FT++D   YC  CF   ++  C+ C K      G K   +  +QWH+ CF C+ C+ S+    F P   +I C  C  N
Sbjct:   62 HEHCFRCFRCDRSLADEPF--TSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 278          
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: rragcb (Ras-related GTP binding Cb [Source:ZFIN;Acc:ZDB-GENE-070705-380])

HSP 1 Score: 250.751 bits (639), Expect = 3.024e-73
Identity = 122/301 (40.53%), Postives = 172/301 (57.14%), Query Frame = 3
Query:  858 FTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDD 1760
              +  D     E F C  C +SL G +YI  ++ P C+ C++    NTC +C+E I  + K+L+FK+ ++HE CF C  C  SL + SFT+ ++ L C +C+  +FSS C  C KI   G K  E+ G  WHEDCF+C  C + IG +SF+P   +  C+ CYE +   +C  C++ + K G++Y+G  WH+ECFLCT C   L+   FTS+ E PYC +CF  L+ K+C  C   I+          GFG  K+IS  D  WH  CF CS+C  S+VG GFF   DQ++C  C    NDD
Sbjct:  436 LPERTDASIMSERFDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAIT----------GFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC----NDD 722          

HSP 2 Score: 137.502 bits (345), Expect = 1.779e-33
Identity = 81/275 (29.45%), Postives = 126/275 (45.82%), Query Frame = 3
Query:  711 FECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEH-FACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSG----ITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTEC 1520
            F+C+ CK  L    Y    +     C+  + +L    C EC E+I + D        ++ EH F C  CD SL+   +   +D   C  C+ N+F + C  C++ +   SK L F    WHE CF+C  C   +   SF   +N  YC  C++ +F+  C  C K    G   Y+  G  WH +CF+C  C+  +  + F        C+ C+ N +A+KC  C+  I   G    I+++   WH+ CF C+ C   L  S F    ++  C +C
Sbjct:  449 FDCRNCKASLYGQQYIKVENEP--HCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQ--GGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC 719          
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000009923.1 (SMESG000009923.1)

HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0
Identity = 551/564 (97.70%), Postives = 553/564 (98.05%), Query Frame = 3
Query:   90 MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDGNENSTE 1781
            MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHV+SLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCD+CHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS          GFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDD NENSTE
Sbjct:    1 MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVNSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDICHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPIS----------GFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGGARNDDANENSTE 554          
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000072127.1 (SMESG000072127.1)

HSP 1 Score: 480.715 bits (1236), Expect = 4.558e-164
Identity = 249/546 (45.60%), Postives = 330/546 (60.44%), Query Frame = 3
Query:  114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSA--EFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  +  H WR  C+ C C   RH    D  D             ++ + +        +  ++ MA    L WLPIGLQ +E+  F  +        + E +      RK  +  QIP  DR   +  +H +   E     Q A  F + R+   LG G +  +D   + C+ C    +   +  +R+ H+A  + + H  CF+C  C E LVD+ YCW ++    +C+RH+ +L   RCA CDELI  +++T+AM+QE H  HFAC  CD+SLTG +YIL+DD P C+ C+E+Q+ N C +CS +I CDSKDLSFK+ HWHEKCF C  C  SLVD+ F TKDN LYC +C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C DC Q IGTKSFIP    I C+ CYE K+AQKC KC  +IR+ G+TYKG PWHKECF CT C K L+  KFTSKEEKPYC EC+ ELF K+C KCT+PI+          GFGGCKFIS  D HWH+ECF C KC  S+VGKGF   +D+++C  CG
Sbjct:   18 CRGFKIHGWRKTCLGCDCSVDRH----DITDESPLP-------ILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKL------RKQKLRRQIPVHDRCVEKF-SHLETPTER----QEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQL-AVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKP--YCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPIT----------GFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 528          
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1)

HSP 1 Score: 479.945 bits (1234), Expect = 9.150e-164
Identity = 256/547 (46.80%), Postives = 338/547 (61.79%), Query Frame = 3
Query:  114 CDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDN-QEV-CSICDKQFEDEDIFCIRITH--SAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            C  +  H WR  C +C C    H    D  D   S    CV   +D + +   +  Q Y  A    +++ L WLPIGL+  E+  FL   +      +   +  +   R   +  QIP  DR   +  +H D   E     Q A  F K+R+++ L    +E +   Q V C  C       ++  +RI H  S   + H +CF C  CKE LVD+ YCW  +   ++C RH+GE    RCA CDELI N+++T+AMDQE H  HFAC  CD+SLTG +YIL+++NP C+KC+E +F NTC +C EKI CDSKDLSFK+ HWHEKCF C  CH SLVDR F TKD  L+C  C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C +C Q IG KSFIP + +I C+ CYENK+AQKC KC+E+IR+ G+TYKG PWHKECF CT+C K L+  KFTSK++KPYC +C+ ELF K+C KCT+PI+          GFGGCKFIS    HWH+ECF CSKC  ++VG+GF   ED ++C  CG
Sbjct:   18 CKGFQLHGWRKTCTACNCSYDIH----DVFDDNIS----CV---LDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFL---SSLPASANPHGVGDDDKSRLRRLRKQIPVQDRRLDKF-SHLDTNVER----QEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSEL-SVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNK--LYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 532          
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1)

HSP 1 Score: 377.481 bits (968), Expect = 2.195e-127
Identity = 176/291 (60.48%), Postives = 220/291 (75.60%), Query Frame = 3
Query:  870 MDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            MDQE H  HFAC  CD+SLTG +YIL+++NP C+KC+E +F NTC +C EKI CDSKDLSFK+ HWHEKCF C  CH SLVDR F TKD  L+C  C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C +C Q IG KSFIP + +I C+ CYENK+AQKC KC+E+IR+ G+TYKG PWHKECF CT+C K L+  KFTSK++KPYC +C+ ELF K+C KCT+PI+          GFGGCKFIS    HWH+ECF CSKC  ++VG+GF   ED ++C  CG
Sbjct:    1 MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPIT----------GFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 281          

HSP 2 Score: 118.242 bits (295), Expect = 2.358e-29
Identity = 66/229 (28.82%), Postives = 112/229 (48.91%), Query Frame = 3
Query: 1068 HEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGIT---YKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCGG 1745
            H   F C  C +SL    +  K+   +C  C++ KF++ C+ C +      K   +  + WHE CF C +C+ S+  + F   +  + C  CY+NK A +C  C+++  K+G+    Y+G  WH+ECFLC  C++ +    F  ++++  C  C+   + ++C KCT+ I             GG   ++     WH ECF C+ C   + G  F  ++D+  C  C G
Sbjct:    6 HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVF-KAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRR-----------GG---VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYG 219          

HSP 3 Score: 106.301 bits (264), Expect = 3.259e-25
Identity = 66/218 (30.28%), Postives = 101/218 (46.33%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFR--TGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGC 1343
            H  CFEC  C   LVD  +   ++S  +FC   +      RC  C ++        +   +++H E F C  C   +    +I RD    CV C+EN++   C KC+E I      +++K   WH++CF C  C   L    FT+KD+  YC +C+ E F+  C  C K      G K   + G+ WH +CF C+ C  ++  + F+     I C  C
Sbjct:   67 HEKCFECFECHTSLVDRPFATKDES--LFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280          
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000074426.1 (SMESG000074426.1)

HSP 1 Score: 358.221 bits (918), Expect = 7.919e-120
Identity = 162/291 (55.67%), Postives = 214/291 (73.54%), Query Frame = 3
Query:  870 MDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLKLLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 1742
            M+ +   E F+C  C  SL+G +YIL+D  P C+ C+E +F N C +C +KI CDSKDLSFKE HWHE CFLC  C +SLVD+ F TKD+ L+CP+C++ KF++ CD CG IF+ G +KYEY G+QWHE+CF+CA+C Q IG KSFIP + +  C+ CYENK AQ+C KC+++IRK G+TYKG+PWHKECFLCT C + L+  KFTSK++KP+C +C+  LF K+C KCT+PI+          GFGGCKFIS+ D HWH+ECF CSKC  S+VG+GF   +D ++C  CG
Sbjct:    1 MENQNTQEIFSCQNCQKSLSGNRYILKDKLPHCLPCYEEKFANKCDRCKDKIGCDSKDLSFKERHWHEACFLCSSCSESLVDKPFATKDDLLFCPDCYESKFATRCDACGNIFKAGARKYEYRGRQWHENCFVCAECQQPIGAKSFIPRDNQTVCVSCYENKFAQRCTKCNDVIRKGGVTYKGEPWHKECFLCTECHQQLASLKFTSKDDKPFCADCYANLFSKKCTKCTKPIT----------GFGGCKFISLDDRHWHSECFTCSKCDTSLVGRGFLTDKDCIICPECG 281          

HSP 2 Score: 107.842 bits (268), Expect = 1.101e-25
Identity = 67/221 (30.32%), Postives = 103/221 (46.61%), Query Frame = 3
Query:  699 HISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELI-VNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRT--GVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYEN 1352
            H +CF C +C E LVD  +   +D   +FC   +      RC  C  +        +   +++H   F C  C   +    +I RD+   CV C+EN+F   C KC++ I      +++K   WH++CFLC  CH  L    FT+KD+  +C +C+   FS  C  C K      G K      + WH +CF C+ C+ S+  + F+     I C  C +N
Sbjct:   67 HEACFLCSSCSESLVDKPFATKDD--LLFCPDCYESKFATRCDACGNIFKAGARKYEYRGRQWHENCFVCAECQQPIGAKSFIPRDNQTVCVSCYENKFAQRCTKCNDVIR--KGGVTYKGEPWHKECFLCTECHQQLASLKFTSKDDKPFCADCYANLFSKKCTKCTKPITGFGGCKFISLDDRHWHSECFTCSKCDTSLVGRGFLTDKDCIICPECGQN 283          
The following BLAST results are available for this feature:
BLAST of Four and A half lim domains vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FHL26.836e-9548.42four and a half LIM domains 2 [Source:HGNC Symbol;... [more]
FHL26.836e-9548.42four and a half LIM domains 2 [Source:HGNC Symbol;... [more]
FHL26.836e-9548.42four and a half LIM domains 2 [Source:HGNC Symbol;... [more]
FHL26.836e-9548.42four and a half LIM domains 2 [Source:HGNC Symbol;... [more]
FHL26.836e-9548.42four and a half LIM domains 2 [Source:HGNC Symbol;... [more]
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BLAST of Four and A half lim domains vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lim-94.662e-13338.53LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5... [more]
lim-94.662e-13338.53LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5... [more]
lim-92.185e-13238.53LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5... [more]
lim-95.502e-13240.95LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5... [more]
lim-92.347e-13138.46LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5... [more]
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BLAST of Four and A half lim domains vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lmpt1.253e-13840.50gene:FBgn0261565 transcript:FBtr0302779[more]
Lmpt1.253e-13840.50gene:FBgn0261565 transcript:FBtr0302781[more]
Lmpt1.253e-13840.50gene:FBgn0261565 transcript:FBtr0306704[more]
Lmpt4.333e-13540.56gene:FBgn0261565 transcript:FBtr0332703[more]
Lmpt2.654e-13440.56gene:FBgn0261565 transcript:FBtr0302780[more]
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BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fhl2b6.192e-9347.72four and a half LIM domains 2b [Source:ZFIN;Acc:ZD... [more]
fhl2b6.192e-9347.72four and a half LIM domains 2b [Source:ZFIN;Acc:ZD... [more]
fhl2b6.192e-9347.72four and a half LIM domains 2b [Source:ZFIN;Acc:ZD... [more]
fhl2a5.968e-9147.37four and a half LIM domains 2a [Source:ZFIN;Acc:ZD... [more]
fhl2a5.968e-9147.37four and a half LIM domains 2a [Source:ZFIN;Acc:ZD... [more]
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BLAST of Four and A half lim domains vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fhl21.574e-9448.42four and a half LIM domains 2 [Source:NCBI gene;Ac... [more]
fhl52.343e-8645.80four and a half LIM domains 5 [Source:NCBI gene;Ac... [more]
shroom39.051e-8445.58shroom family member 3 [Source:Xenbase;Acc:XB-GENE... [more]
fhl13.703e-6738.46four and a half LIM domains 1 [Source:NCBI gene;Ac... [more]
fhl14.450e-6738.46four and a half LIM domains 1 [Source:NCBI gene;Ac... [more]
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BLAST of Four and A half lim domains vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Fhl21.662e-9348.07four and a half LIM domains 2 [Source:MGI Symbol;A... [more]
Fhl21.662e-9348.07four and a half LIM domains 2 [Source:MGI Symbol;A... [more]
Fhl31.261e-8543.71four and a half LIM domains 3 [Source:MGI Symbol;A... [more]
Fhl31.018e-8243.36four and a half LIM domains 3 [Source:MGI Symbol;A... [more]
Fhl52.302e-8043.10four and a half LIM domains 5 [Source:MGI Symbol;A... [more]
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BLAST of Four and A half lim domains vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q14192|FHL2_HUMAN3.283e-9448.42Four and a half LIM domains protein 2 OS=Homo sapi... [more]
sp|O70433|FHL2_MOUSE1.162e-9248.07Four and a half LIM domains protein 2 OS=Mus muscu... [more]
sp|O35115|FHL2_RAT1.922e-9247.72Four and a half LIM domains protein 2 OS=Rattus no... [more]
sp|Q2KI95|FHL2_BOVIN2.206e-9147.37Four and a half LIM domains protein 2 OS=Bos tauru... [more]
sp|Q3ZBI6|FHL3_BOVIN1.010e-8644.76Four and a half LIM domains protein 3 OS=Bos tauru... [more]
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BLAST of Four and A half lim domains vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
B1AAM61.807e-16445.45LIMPETin OS=Schistosoma mansoni OX=6183 PE=2 SV=1[more]
U6J6Y32.053e-16444.64Four and a half LIM domains protein 2 OS=Echinococ... [more]
A0A068Y1Z92.314e-16444.64Four and A half lim domains OS=Echinococcus multil... [more]
A0A0R3W1E08.052e-16444.58Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A0R3X1E44.978e-16344.46Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
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BLAST of Four and A half lim domains vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fhl2a2.524e-9147.02four and a half LIM domains protein 2 [Source:NCBI... [more]
fhl3a2.689e-8745.42four and a half LIM domains 3 [Source:NCBI gene;Ac... [more]
fhl2b1.909e-7142.11four and a half LIM domains 2b [Source:ZFIN;Acc:ZD... [more]
fhl1a5.988e-6338.68four and a half LIM domains 1 [Source:NCBI gene;Ac... [more]
fhl1a6.303e-6338.68four and a half LIM domains 1 [Source:NCBI gene;Ac... [more]
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BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fhl51.696e-4844.77four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB... [more]
fhl51.531e-4643.35four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB... [more]
fhl3b6.549e-4635.02four and a half LIM domains 3b [Source:ZFIN;Acc:ZD... [more]
PXN1.872e-2728.32paxillin [Source:HGNC Symbol;Acc:HGNC:9718][more]
PXN4.691e-2731.56paxillin [Source:HGNC Symbol;Acc:HGNC:9718][more]
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BLAST of Four and A half lim domains vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
LRG11.894e-631.13GTPase-activating protein (GAP); contains Rho1p-sp... [more]
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BLAST of Four and A half lim domains vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO411001.310e-9030.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
LIMPET4.655e-8430.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO497981.990e-3729.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO411819.084e-3231.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498013.254e-3025.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Four and A half lim domains vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fhl2a7.024e-9247.37four and a half LIM domains protein 2 [Source:NCBI... [more]
fhl56.656e-8945.77four and a half LIM domains protein 2-like [Source... [more]
fhl3a8.221e-8845.05four and a half LIM domains 3 [Source:NCBI gene;Ac... [more]
fhl3a7.685e-8746.13four and a half LIM domains 3 [Source:NCBI gene;Ac... [more]
rragcb3.024e-7340.53Ras-related GTP binding Cb [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Four and A half lim domains vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009923.10.000e+097.70SMESG000009923.1[more]
SMESG000072127.14.558e-16445.60SMESG000072127.1[more]
SMESG000037769.19.150e-16446.80SMESG000037769.1[more]
SMESG000037769.12.195e-12760.48SMESG000037769.1[more]
SMESG000074426.17.919e-12055.67SMESG000074426.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025952 ID=SMED30025952|Name=Four and A half lim domains|organism=Schmidtea mediterranea sexual|type=transcript|length=1930bp
AAATTATTTAAAGACTTTAAAAGTGAAAAAAAAGTTTAGTGGTATTTTAT
AGGGTGATTTAAATTTATTTCATGAATTATAAACTATAAATGGATTGTAA
AATATGCAAATCATGCGATAAGTACACAAATCATAAATGGCGCTTCCGAT
GCGTCAGTTGTCGGTGTCCCAACTACCGCCATGTGAGCCATGTAGACTCC
CTGGATAGCGGAAGCAGCACAGGACAGCATTGCGTCAACAACTGGATCGA
CAGCCTGACCATGGAATCTCACAACCAGCTCCAAGACTACAAGGCAGCTA
TCTCCATGGCCGAATCTAATAATCTAGTCTGGTTACCTATAGGCCTTCAA
CAAGCAGAGATTTCGGATTTCCTTCGAGTTTGGAATGATTTCGGGCCAGG
ATTTTCTCTGGAAAACATTCCCATCAATCAACTTGATAGGAAAATGAATA
TTTTCTATCAGATTCCAGATTGTGATAGAATTTCTTCAGAAGTCCAGAAT
CATTTTGATGACAAAGACGAGGATAAATTGATAGTTCAGCTAGCTCGAGC
TTTCAAGAAGAGTCGTGATACGAACTATTTGGGATTCGGTGAAATAGAGC
CAGTCGATAACCAAGAGGTGTGCTCCATTTGCGATAAGCAATTCGAGGAT
GAAGACATTTTCTGTATTCGAATCACTCATTCCGCCGAATTCGCGTCTCA
TATTTCTTGCTTTGAGTGCAAAACTTGTAAAGAGAAGTTGGTGGATTTCC
ACTACTGCTGGAGCAATGATTCCAAAGCGATCTTTTGCCTGAGACATTTC
GGAGAGCTCACTTACTGCCGATGTGCTGAATGTGACGAGCTTATTGTTAA
TAAAGACTTTACCAAAGCGATGGATCAAGAATTTCATGTTGAGCATTTCG
CCTGCCATGTTTGTGATTCCTCACTTACGGGGACCAAATACATTTTGCGT
GATGACAATCCGTTTTGTGTGAAGTGCTTTGAAAATCAGTTCGGTAACAC
TTGTCACAAATGTTCAGAAAAGATCAGTTGTGACAGCAAGGATTTATCGT
TTAAAGAGTGGCACTGGCATGAGAAATGTTTCCTTTGCGATCTTTGTCAC
GACTCGCTAGTTGACAGATCATTCACAACAAAAGACAATTATTTATATTG
TCCAAATTGTTTCGATGAAAAGTTTTCCTCCTGTTGCGATGGTTGTGGGA
AAATTTTCAGAACCGGCGTGAAGAAGTATGAGTATGCCGGCAAACAATGG
CACGAAGATTGCTTCATTTGTGCCGACTGCAACCAAAGCATCGGGACCAA
GAGTTTCATTCCTATCAATGGCAAAATCAGTTGCATTGGTTGCTATGAAA
ATAAGCACGCTCAAAAATGTTTCAAATGTTCAGAAATAATTCGCAAAAGT
GGGATCACTTACAAAGGTGACCCGTGGCACAAGGAGTGCTTCCTGTGCAC
GAGCTGTCAGAAAATGCTAAGTGAGTCGAAGTTCACTTCGAAAGAAGAGA
AACCATATTGCACGGAATGTTTTATAGAATTATTCTGTAAGCGCTGCAAA
AAATGCACCCAACCGATTTCCGATACTAAATTTTTAAAACTTCTAATGAA
AGGTTTTGGTGGCTGTAAATTTATCTCAGTTGGTGACGACCATTGGCATA
CTGAATGTTTCAATTGTTCTAAATGCCAGGCAAGCATGGTAGGCAAAGGA
TTTTTCCTTCAAGAAGATCAAATGGTCTGTGGCAGTTGTGGAGGCGCCAG
AAATGATGATGGCAATGAGAATTCAACTGAATAACTTTTATTAATTTATA
TTTTTCAGCAGTAATGTTGGGAAAAATTTTTATATTCCATGGTAACGGTG
GACAGGAATTCGGTTTTCATATTTTAACATATAAATTAATAAAAAAATTA
TATTGCAATTTGCTAAATTTTGTGTTGTCT
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protein sequence of SMED30025952-orf-1

>SMED30025952-orf-1 ID=SMED30025952-orf-1|Name=SMED30025952-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=565bp
MDCKICKSCDKYTNHKWRFRCVSCRCPNYRHVSHVDSLDSGSSTGQHCVN
NWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWND
FGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQ
LARAFKKSRDTNYLGFGEIEPVDNQEVCSICDKQFEDEDIFCIRITHSAE
FASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDE
LIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQ
FGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDLCHDSLVDRSFTTKDN
YLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQS
IGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKEC
FLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISDTKFLK
LLMKGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSC
GGARNDDGNENSTE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR037987FHL2/3/5
IPR001781Znf_LIM
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001781Zinc finger, LIM-typeSMARTSM00132lim_4coord: 304..357
e-value: 3.2E-9
score: 46.6
coord: 365..418
e-value: 5.0E-12
score: 56.0
coord: 426..477
e-value: 9.4E-14
score: 61.7
coord: 177..236
e-value: 0.2
score: 4.9
coord: 485..550
e-value: 1.7E-10
score: 50.9
coord: 244..296
e-value: 1.7E-13
score: 60.8
IPR001781Zinc finger, LIM-typePFAMPF00412LIMcoord: 245..300
e-value: 8.8E-12
score: 45.1
coord: 486..550
e-value: 2.2E-8
score: 34.2
coord: 427..481
e-value: 1.7E-12
score: 47.3
coord: 366..422
e-value: 6.4E-11
score: 42.3
coord: 305..361
e-value: 1.1E-8
score: 35.2
IPR001781Zinc finger, LIM-typePROSITEPS00478LIM_DOMAIN_1coord: 366..401
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 304..364
score: 10.346
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 485..557
score: 9.583
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 176..242
score: 8.078
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 425..484
score: 12.959
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 366..424
score: 7.712
IPR001781Zinc finger, LIM-typePROSITEPS50023LIM_DOMAIN_2coord: 243..303
score: 12.563
NoneNo IPR availableGENE3DG3DSA:2.10.110.10coord: 168..243
e-value: 7.3E-6
score: 27.6
NoneNo IPR availableGENE3DG3DSA:2.10.110.10coord: 480..551
e-value: 1.3E-13
score: 52.5
NoneNo IPR availableGENE3DG3DSA:2.10.110.10coord: 420..479
e-value: 2.2E-19
score: 71.1
NoneNo IPR availableGENE3DG3DSA:2.10.110.10coord: 298..359
e-value: 1.8E-18
score: 68.3
coord: 360..419
e-value: 8.2E-16
score: 59.8
NoneNo IPR availableGENE3DG3DSA:2.10.110.10coord: 244..297
e-value: 1.5E-13
score: 52.6
NoneNo IPR availablePANTHERPTHR24205:SF4LIM DOMAIN FAMILYcoord: 21..550
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 333..398
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 272..336
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 453..529
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 394..456
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 210..276
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 525..552
IPR037987Four and a half LIM domains protein 2/3/5PANTHERPTHR24205FAMILY NOT NAMEDcoord: 21..550