GTP cyclohydrolase

Overview
NameGTP cyclohydrolase
Smed IDSMED30025911
Length (bp)586
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GTP cyclohydrolase (SMED30025911) t-SNE clustered cells

Violin plots show distribution of expression levels for GTP cyclohydrolase (SMED30025911) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GTP cyclohydrolase (SMED30025911) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GTP cyclohydrolase (SMED30025911) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30025911

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30025911SMESG000049600.1 SmedASXL_002956SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GTP cyclohydrolase vs. Ensembl Celegans
Match: T24A6.7 (pep chromosome:WBcel235:V:3525274:3526178:1 gene:WBGene00020747.1 transcript:T24A6.7.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T24A6.7)

HSP 1 Score: 121.709 bits (304), Expect = 1.302e-34
Identity = 66/167 (39.52%), Postives = 96/167 (57.49%), Query Frame = 1 
Query:   79 KYIMFYK-NSVFSQFYACNFTVN--------NQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENV--WIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSEN 546
            +++ FYK + VFS FY C F+          N  FNC  QY MY KALL  D + A KILKE  P  +K+LGRQ+    + +  W +N ++++  G   KF+QN     +L  T G +LVE +P D IWGIG S  D    +E+ WRG N +G ++  VR+E+ +++
Sbjct:   14 QFVFFYKFDCVFSNFYPCEFSAQEFENDVEKNVNFNCSEQYFMYHKALLVGDSEIAGKILKETDPRKMKRLGRQLSMSKKQLEEWSQNSKEVMYNGCLEKFSQNNVCRENLFRTHGMRLVEASPMDRIWGIGLSHHDKRCENEQTWRGTNWMGSVLDKVREELWTKS 180
[Close]
BLAST of GTP cyclohydrolase vs. Ensembl Celegans
Match: irg-1 (Infection Response protein [Source:UniProtKB/TrEMBL;Acc:O16327])

HSP 1 Score: 110.538 bits (275), Expect = 3.103e-30
Identity = 65/168 (38.69%), Postives = 91/168 (54.17%), Query Frame = 1 
Query:   82 YIMFYKNS-VFSQFYACNF---TVNN-----------QKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENV--WIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEI 534
            +++FY+ + VFS FY   F    V N            KF C  QY MY KALL  D   A +ILKE  P+ +K LGR++    E +  W +  + ++      KF+QN+D  + L  T G KLVE +P D IWGIG  + D    DE+ WRG N LG+++  VR+E+
Sbjct:   15 FVLFYEAACVFSNFYPSGFEAKPVENFLKDTEKKEKLLKFTCSEQYFMYNKALLVGDMDIAEQILKETNPMKMKLLGRKLSMTKEQLKLWSQKSKDVMYRACLEKFSQNEDCRMILFRTHGMKLVEASPTDKIWGIGLDKADQRCEDERNWRGSNWLGEVLDQVREEL 182
[Close]
BLAST of GTP cyclohydrolase vs. Ensembl Celegans
Match: C08E8.4 (pep chromosome:WBcel235:V:18371846:18373528:1 gene:WBGene00007440.1 transcript:C08E8.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C08E8.4)

HSP 1 Score: 99.7525 bits (247), Expect = 4.415e-26
Identity = 62/179 (34.64%), Postives = 89/179 (49.72%), Query Frame = 1 
Query:   64 AFTNEKYIMFYKN-SVFSQFYACNFTVNNQK---------------FNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQV----HDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILS 540
            A   + Y++FY++ SVFS F+  NF     K               FNC  QY MY KALL  D  +A  I+  + P+++K  GRQ+    HD +   W +    I+     AKF+QN +L   L  T+   LVE + ND IWG G  + D  + D   W G N LG+I+  +R E++ 
Sbjct:    8 AKNQQTYVLFYEDESVFSNFHPSNFEAETAKKLVNSGMFKEEEMLKFNCSEQYFMYHKALLVGDVDSAKVIITAKHPMVMKMTGRQLNMNRHDIDN--WTQKSRDIMYLACLAKFSQNVELRKMLFRTQDMYLVEASGNDAIWGNGIWKEDKRSDDVANWHGTNYLGEILDRIRGELIG 184
[Close]
BLAST of GTP cyclohydrolase vs. Ensembl Celegans
Match: C37C3.1 (pep chromosome:WBcel235:V:7857963:7859909:1 gene:WBGene00016495.1 transcript:C37C3.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C37C3.1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.643e-20
Identity = 56/169 (33.14%), Postives = 88/169 (52.07%), Query Frame = 1 
Query:   70 TNEKYIMFYKN-SVFSQFYACNFTVNN-------QKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQ--VHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKW----RGLNLLGKIISDVRDEI 534
            ++ +Y +F++  S FS  +  +F  +        + F C  QY MY KA L  D K    IL+   P  +K +  +  +  + ++VW EN+E++++ G  AKF  ++ L   L  T GSKLVE +P D IWGIG      +     +W     G NLLGKI+  +R+E+
Sbjct:   18 SDGEYTLFFRKVSPFSNHHPADFICDEAINCKEPKTFCCSEQYYMYNKASLLMDSKLMAAILEATCPKTMKTMCSKWSLKGWKDSVWDENKEEVMRIGCLAKFRASRHLRYALFLTTGSKLVECSPFDKIWGIG------KDMQAAQWAPLSSGKNLLGKILDGIREEL 180
[Close]
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Match: sp|Q5UR67|RIBX_MIMIV (N-glycosidase R617 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R617 PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.374e-31
Identity = 69/164 (42.07%), Postives = 96/164 (58.54%), Query Frame = 1 
Query:   76 EKYIMFY---KNS----VFSQFYACNFT--VNNQKFN---CCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDE 531
            +KY+ F+   KN     +FSQ++  NF   +N ++F       QYMM  KALLF D     KI++  K   IK LGR+V +FN  +W +++  IV EGNR KF+QN DL+  LL T    +VE +P+D IWGIG +        E KW G NLLGK++  +R+E
Sbjct:    4 DKYVFFHGANKNQAGVHIFSQWFPVNFKEYLNGEEFAEYVSAEQYMMAHKALLFGDMFHFKKIMECSKQCKIKYLGRRVRNFNPTIWDKHKFDIVTEGNRLKFSQNPDLMKRLLETGNKTIVEASPSDKIWGIGLTAQQAVNIPENKWPGKNLLGKVLMKIREE 167
[Close]
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Match: sp|P30176|RIBX_ECOLI (N-glycosidase YbiA OS=Escherichia coli (strain K12) OX=83333 GN=ybiA PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.421e-10
Identity = 44/157 (28.03%), Postives = 73/157 (46.50%), Query Frame = 1 
Query:   85 IMFYKNSV----FSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSE 543
            I FY  S     FS F A    V+ + +     Y   +K   F D+K   +I +   P++  ++GR         W   +E+++++  RAKF Q+ +L   LL+T  +KLVE   ND  WG           D    +G N LG ++ ++R+++  E
Sbjct:   17 INFYSTSDDYGDFSNFAAWPIKVDGKTWPTSEHYFQAQK---FLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALRAKFEQHAELRALLLATAPAKLVEHTENDAYWG-----------DGGHGKGKNRLGYLLMELREQLAIE 159
[Close]
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Match: sp|Q7MGG3|RIBXA_VIBVY (Riboflavin biosynthesis protein VVA0006 OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=VVA0006 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.970e-9
Identity = 50/177 (28.25%), Postives = 79/177 (44.63%), Query Frame = 1 
Query:   40 YILNSAIMAFTNEKYIMFYK----NSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN 558
            Y + S IM    E+ I FY+    +   + FY C+ TV+     C      Y +A  F D +   K+L+        +L R+   +  + W + R ++++   R KF QN  L   LL+T  ++L E +  D  WG           D     G N LG+I+  VR+E L E++  N
Sbjct:   10 YDVRSNIM----EQPIYFYEPDENHGFLANFYPCSITVSG---TCWPSSEHYYQAQKFDDVRLQEKVLRAEDAAQAFRLSREYQQWQRHDWYDIRVEVMRFIVREKFLQNTPLAHQLLATGDTELKEHSHKDAFWG-----------DGGDGHGRNELGRILMMVREE-LQEHAPYN 167
[Close]
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Match: sp|Q3Z3Y7|RIBX_SHISS (N-glycosidase YbiA OS=Shigella sonnei (strain Ss046) OX=300269 GN=ybiA PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 5.041e-8
Identity = 36/120 (30.00%), Postives = 58/120 (48.33%), Query Frame = 1 
Query:   85 IMFYKNSV----FSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIW 432
            I FY  S     FS F A    V+ + +     Y   +K   F D+K   +I +   P++  ++GR         W   +E+++++  RAKF Q+ +L + LL+T  +KLVE   ND  W
Sbjct:   17 INFYSTSDDYGDFSNFAAWPIKVDGKTWPTSEHYFQAQK---FLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALRAKFEQHAELRVLLLATAPAKLVEHTENDAYW 133
[Close]
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Match: sp|Q323Z6|RIBX_SHIBS (N-glycosidase YbiA OS=Shigella boydii serotype 4 (strain Sb227) OX=300268 GN=ybiA PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 8.793e-8
Identity = 36/120 (30.00%), Postives = 57/120 (47.50%), Query Frame = 1 
Query:   85 IMFYKNSV----FSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIW 432
            I FY  S     FS F A    V+ + +     Y   +K   F D+K   +I +   P++  ++GR         W   +E+++++  RAKF Q+ +L   LL+T  +KLVE   ND  W
Sbjct:   17 INFYSTSDDYGDFSNFAAWPIKVDGKTWPTSEHYFQAQK---FLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALRAKFEQHAELRALLLATAPAKLVEHTENDAYW 133
[Close]
BLAST of GTP cyclohydrolase vs. TrEMBL
Match: A0A267FK67 (DUF1768 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008561g1 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 5.491e-53
Identity = 84/155 (54.19%), Postives = 107/155 (69.03%), Query Frame = 1 
Query:   70 TNEKYIMFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEI 534
            T      F K S FSQFY C F V+   F+C  QYMMY+KA LF+D  TA  IL   KP  +K LGRQV  FN++VW E+R +IV  GN AKF QN +LL  LL T+G+ LVE  PND IWGIG +E++P+A D+ KWRGLNLLG+I++ VR+++
Sbjct:    3 TGRFTFFFTKESPFSQFYPCQFKVDGCTFSCAEQYMMYRKAKLFNDHATAEAILAATKPAKMKALGRQVDGFNDDVWKEHRWEIVHSGNMAKFGQNPELLRTLLDTEGTTLVEAAPNDCIWGIGLAESNPDARDQSKWRGLNLLGQILTRVREDL 157
[Close]
BLAST of GTP cyclohydrolase vs. TrEMBL
Match: A0A267FW75 (DUF1768 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008992g1 PE=4 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 6.057e-53
Identity = 84/155 (54.19%), Postives = 107/155 (69.03%), Query Frame = 1 
Query:   70 TNEKYIMFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEI 534
            T      F K S FSQFY C F V+   F+C  QYMMY+KA LF+D  TA  IL   KP  +K LGRQV  FN++VW E+R +IV  GN AKF QN +LL  LL T+G+ LVE  PND IWGIG +E++P+A D+ KWRGLNLLG+I++ VR+++
Sbjct:    3 TGRFTFFFTKESPFSQFYPCQFKVDGCTFSCAEQYMMYRKAKLFNDHATAEAILAATKPAKMKALGRQVDGFNDDVWKEHRWEIVHSGNMAKFGQNPELLRTLLDTEGTTLVEAAPNDCIWGIGLAESNPDARDQAKWRGLNLLGQILTRVREDL 157
[Close]
BLAST of GTP cyclohydrolase vs. TrEMBL
Match: A0A0E3WHQ6 (DUF1768 domain-containing protein OS=Paenibacillus riograndensis SBR5 OX=1073571 GN=PRIO_3300 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 3.679e-50
Identity = 79/157 (50.32%), Postives = 107/157 (68.15%), Query Frame = 1 
Query:   76 EKYIMFYKN-SVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSE 543
            EK+  FY++ S FSQ+Y C FTV+   FNC  QYMMY KALLF D++TA++IL+ R P   K+LGR+V  FN+  W  +   IV +GNR KF QN++LL  LL TKG+ LVE +P D IWG+G +E DP       WRG N LG++++ +RD++L E
Sbjct:    2 EKFTYFYRSGSPFSQWYPCYFTVDEHTFNCAEQYMMYAKALLFRDEETAMQILRARTPRTQKELGRKVSGFNDTEWKNHCRDIVYQGNREKFLQNEELLQQLLDTKGTTLVEASPTDRIWGVGLTEDDPRIRSRSSWRGSNWLGEVLTKLRDDLLQE 158
[Close]
BLAST of GTP cyclohydrolase vs. TrEMBL
Match: A0A132TI25 (GTP cyclohydrolase OS=Paenibacillus riograndensis OX=483937 GN=AMQ84_28310 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 3.679e-50
Identity = 79/157 (50.32%), Postives = 107/157 (68.15%), Query Frame = 1 
Query:   76 EKYIMFYKN-SVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSE 543
            EK+  FY++ S FSQ+Y C FTV+   FNC  QYMMY KALLF D++TA++IL+ R P   K+LGR+V  FN+  W  +   IV +GNR KF QN++LL  LL TKG+ LVE +P D IWG+G +E DP       WRG N LG++++ +RD++L E
Sbjct:    2 EKFTYFYRSGSPFSQWYPCYFTVDEHTFNCAEQYMMYAKALLFRDEETAMQILRARTPRTQKELGRKVSGFNDTEWKNHCRDIVYQGNREKFLQNEELLQQLLDTKGTTLVEASPTDRIWGVGLTEDDPRIRSRSSWRGSNWLGEVLTKLRDDLLQE 158
[Close]
BLAST of GTP cyclohydrolase vs. TrEMBL
Match: A0A1I8JB49 (Sec3-PIP2_bind domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 1.314e-49
Identity = 83/148 (56.08%), Postives = 106/148 (71.62%), Query Frame = 1 
Query:   91 FYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEI 534
            F K S FSQFY C F V+   F+C  QYMMY+KA LF+D  TA  IL   KP  +K LGRQV  FN++VW E+R +IV  GN AKF QN +LL  LL T+G+ LVE  PND IWGIG +E++P+A D+ KWRGLNLLG+I++ VR+++
Sbjct:   10 FTKESPFSQFYPCQFKVDGCTFSCAEQYMMYRKAKLFNDHATAEAILAATKPAKMKALGRQVDGFNDDVWKEHRWEIVHSGNMAKFGQNPELLRTLLDTEGTTLVEAAPNDCIWGIGLAESNPDARDQSKWRGLNLLGQILTRVREDL 157
[Close]
BLAST of GTP cyclohydrolase vs. Planmine SMEST
Match: SMESG000049600.1 (SMESG000049600.1)

HSP 1 Score: 325.094 bits (832), Expect = 2.467e-115
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1 
Query:   88 MFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN 558
            MFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN
Sbjct:    1 MFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN 157
[Close]
BLAST of GTP cyclohydrolase vs. Planmine SMEST
Match: SMESG000049600.1 (SMESG000049600.1)

HSP 1 Score: 261.151 bits (666), Expect = 1.376e-90
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 1 
Query:  175 MMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN 558
            MMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN
Sbjct:    1 MMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENSTAN 128
[Close]
BLAST of GTP cyclohydrolase vs. Planmine SMEST
Match: SMESG000049592.1 (SMESG000049592.1)

HSP 1 Score: 261.922 bits (668), Expect = 2.996e-90
Identity = 124/158 (78.48%), Postives = 137/158 (86.71%), Query Frame = 1 
Query:   76 EKYIMFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDDKTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNKDLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKIISDVRDEILSENS 549
            +KY MF+  SVFSQFY C FTVN+QKF  C QYMMY KALLFHD+  A KIL+ER P IIK+LGRQVH F+E +W ENRE+IVKEGN+AKFTQNKDL  +LLSTKG+KLVETNPNDLIWGIGYSETDPEA DE KWRGLNLLGKII+DVRDEILSENS
Sbjct:    5 DKYTMFFSRSVFSQFYTCTFTVNDQKFISCEQYMMYSKALLFHDENIARKILQERNPKIIKKLGRQVHKFDEKIWKENRERIVKEGNKAKFTQNKDLQKELLSTKGTKLVETNPNDLIWGIGYSETDPEALDETKWRGLNLLGKIITDVRDEILSENS 162
The following BLAST results are available for this feature:
BLAST of GTP cyclohydrolase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
T24A6.71.302e-3439.52pep chromosome:WBcel235:V:3525274:3526178:1 gene:W... [more]
irg-13.103e-3038.69Infection Response protein [Source:UniProtKB/TrEM... [more]
C08E8.44.415e-2634.64pep chromosome:WBcel235:V:18371846:18373528:1 gene... [more]
C37C3.12.643e-2033.14pep chromosome:WBcel235:V:7857963:7859909:1 gene:W... [more]
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5UR67|RIBX_MIMIV1.374e-3142.07N-glycosidase R617 OS=Acanthamoeba polyphaga mimiv... [more]
sp|P30176|RIBX_ECOLI2.421e-1028.03N-glycosidase YbiA OS=Escherichia coli (strain K12... [more]
sp|Q7MGG3|RIBXA_VIBVY3.970e-928.25Riboflavin biosynthesis protein VVA0006 OS=Vibrio ... [more]
sp|Q3Z3Y7|RIBX_SHISS5.041e-830.00N-glycosidase YbiA OS=Shigella sonnei (strain Ss04... [more]
sp|Q323Z6|RIBX_SHIBS8.793e-830.00N-glycosidase YbiA OS=Shigella boydii serotype 4 (... [more]
back to top
BLAST of GTP cyclohydrolase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FK675.491e-5354.19DUF1768 domain-containing protein OS=Macrostomum l... [more]
A0A267FW756.057e-5354.19DUF1768 domain-containing protein OS=Macrostomum l... [more]
A0A0E3WHQ63.679e-5050.32DUF1768 domain-containing protein OS=Paenibacillus... [more]
A0A132TI253.679e-5050.32GTP cyclohydrolase OS=Paenibacillus riograndensis ... [more]
A0A1I8JB491.314e-4956.08Sec3-PIP2_bind domain-containing protein OS=Macros... [more]
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GTP cyclohydrolase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000049600.12.467e-115100.00SMESG000049600.1[more]
SMESG000049600.11.376e-90100.00SMESG000049600.1[more]
SMESG000049592.12.996e-9078.48SMESG000049592.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025911 ID=SMED30025911|Name=GTP cyclohydrolase|organism=Schmidtea mediterranea sexual|type=transcript|length=586bp
CTTGATTATTTATATTTTCGCTTTATTTTGGTTAAATAATATATTTTAAA
TAGCGCAATTATGGCTTTTACAAATGAAAAATATATTATGTTTTATAAAA
ATTCAGTTTTCAGTCAATTTTATGCATGCAATTTTACTGTAAATAATCAG
AAGTTCAATTGCTGTTTGCAATATATGATGTATAAAAAAGCATTATTGTT
TCACGATGACAAAACCGCCGTAAAAATATTAAAAGAAAGAAAGCCAGTAA
TTATCAAACAACTTGGTCGGCAAGTACATGATTTTAATGAGAATGTCTGG
ATAGAAAATCGTGAGAAGATCGTTAAAGAGGGAAATAGAGCGAAATTCAC
TCAAAATAAGGATCTTTTATTAGACTTATTAAGCACAAAGGGTTCAAAGC
TCGTGGAAACTAATCCAAATGATTTGATATGGGGTATCGGATATTCCGAA
ACCGATCCGGAAGCGTTCGATGAGAAAAAGTGGCGAGGATTGAATTTATT
GGGAAAAATTATATCGGACGTTCGAGATGAAATTTTATCTGAAAACTCAA
CTGCTAATTGATTTTAATAAATGTTTAATTTCTTTG
back to top

protein sequence of SMED30025911-orf-1

>SMED30025911-orf-1 ID=SMED30025911-orf-1|Name=SMED30025911-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=167bp
MAFTNEKYIMFYKNSVFSQFYACNFTVNNQKFNCCLQYMMYKKALLFHDD
KTAVKILKERKPVIIKQLGRQVHDFNENVWIENREKIVKEGNRAKFTQNK
DLLLDLLSTKGSKLVETNPNDLIWGIGYSETDPEAFDEKKWRGLNLLGKI
ISDVRDEILSENSTAN*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR037238YbiA-like_sf
IPR012816NADAR
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012816NADARTIGRFAMTIGR02464TIGR02464coord: 9..158
e-value: 1.3E-45
score: 153.4
IPR012816NADARPFAMPF08719DUF1768coord: 11..158
e-value: 9.7E-48
score: 162.4
IPR012816NADARCDDcd15457NADARcoord: 7..156
e-value: 4.06461E-59
score: 178.962
IPR037238YbiA-like superfamilyGENE3DG3DSA:1.10.357.40coord: 1..165
e-value: 1.2E-37
score: 130.9
IPR037238YbiA-like superfamilySUPERFAMILYSSF143990YbiA-likecoord: 3..161