SMED30025898
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025898 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30025898 aligns in the following genomic locations:
Homology
BLAST of SMED30025898 vs. TrEMBL
Match: A0A5J4NY81 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0005698 PE=4 SV=1) HSP 1 Score: 63.929 bits (154), Expect = 5.832e-14 Identity = 29/81 (35.80%), Postives = 42/81 (51.85%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFE 381 N W QS Q+KNADI++ + N KG T+P+S+ YRYH + CLS ++K+Y+ E +RFE Sbjct: 223 NLAFWIQQSFQLKNADILFLME--------------NDKGTYGWILFVVTMPISLFYRYHCTVCLSQCFNKLYEDETQRFE 289 HSP 2 Score: 42.3578 bits (98), Expect = 5.832e-14 Identity = 20/42 (47.62%), Postives = 26/42 (61.90%), Query Frame = 3 Query: 12 LEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 LE+IQ IQTH + D+F RCS KPGR ++ L+GI Sbjct: 181 LELIQILIQTHMIQDSFHRCSDKVKHQITKPGRQSIVALLGI 222
BLAST of SMED30025898 vs. TrEMBL
Match: A0A5J4NC21 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0005650 PE=4 SV=1) HSP 1 Score: 58.5362 bits (140), Expect = 6.696e-13 Identity = 30/85 (35.29%), Postives = 51/85 (60.00%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLR 393 N +W + S Q+K+ D+++ I +F QD++ ++GL F+ LP+ ML+RYHSS CL+ + ++Y+ E R+E LR Sbjct: 268 NFSLWMIYSFQVKHNDVLF----RISWNKF-QDAD--LRGL--HMFVTVVLPMVMLFRYHSSVCLAISFVRIYEDEVTRYESMLR 343 HSP 2 Score: 44.2838 bits (103), Expect = 6.696e-13 Identity = 19/45 (42.22%), Postives = 27/45 (60.00%), Query Frame = 3 Query: 3 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 S LE+IQ +QT+F+ D F RC + + KPGRT + IL + Sbjct: 223 STLLELIQAMVQTYFIHDMFYRCCHHKIYQQTKPGRTVIVILSAL 267
BLAST of SMED30025898 vs. TrEMBL
Match: A0A5J4P2A0 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0000801 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 7.370e-13 Identity = 55/195 (28.21%), Postives = 92/195 (47.18%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSE----NNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLR---AGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLL----------EDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLK 672 N +W + S Q+KN I+ F Q++E + ++GL + F++ LP+ ML+RYHSS CL+ ++Y+ E R+E LR G T EF++K + + + +E + LL ED G+ +K G P T E RR T +E+A ++ + E + +L ++ LK Sbjct: 519 NFSLWMIYSFQVKNNSIL-----------FKQNAEAVEASGLQGLTI--FIRVVLPMVMLFRYHSSVCLAISVVRIYEDEVARYESMLRWVKQGTTR-------EFLQKQSFDFRHI-WETGGSSLLNTTKDVQLSSEDPVEGKLTKLDGT-PKQTTTKSEGHGGRRNTEFTLEVARTRLAAIEENHRLTEQKLK 691 HSP 2 Score: 39.2762 bits (90), Expect = 7.370e-13 Identity = 17/45 (37.78%), Postives = 26/45 (57.78%), Query Frame = 3 Query: 3 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 S LE++Q +QT+F+ D F RC + + KPGR + IL + Sbjct: 474 SILLELMQVIVQTYFIHDMFYRCCHHEVYQQTKPGRAVIIILSAL 518
BLAST of SMED30025898 vs. TrEMBL
Match: A0A419PT59 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_10723s PE=4 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 1.218e-12 Identity = 31/81 (38.27%), Postives = 46/81 (56.79%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFE 381 N +W QS Q+KNADII FL +E + G ++ T+P+S+ YRYH + CLS ++K+Y+ E +RFE Sbjct: 433 NLALWIQQSFQLKNADII-----------FLLPNEKDSYGWIL---FVVTMPISLFYRYHCTVCLSQCFNKLYEDETQRFE 499 HSP 2 Score: 36.5798 bits (83), Expect = 1.218e-12 Identity = 17/42 (40.48%), Postives = 25/42 (59.52%), Query Frame = 3 Query: 12 LEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 LE++Q +QT+ + D F RCS KPGR ++ L+GI Sbjct: 391 LELLQIVVQTYMIQDCFHRCSDKLTHQIIKPGRQSIVALLGI 432
BLAST of SMED30025898 vs. TrEMBL
Match: A0A4S2LXR8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005503 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 1.280e-12 Identity = 31/81 (38.27%), Postives = 46/81 (56.79%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFE 381 N +W QS Q+KNADII FL +E + G ++ T+P+S+ YRYH + CLS ++K+Y+ E +RFE Sbjct: 398 NLALWIQQSFQLKNADII-----------FLLPNEKDSYGWIL---FVVTMPISLFYRYHCTVCLSQCFNKLYEDETQRFE 464 HSP 2 Score: 36.5798 bits (83), Expect = 1.280e-12 Identity = 17/42 (40.48%), Postives = 25/42 (59.52%), Query Frame = 3 Query: 12 LEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 LE++Q +QT+ + D F RCS KPGR ++ L+GI Sbjct: 356 LELLQIVVQTYMIQDCFHRCSDKLTHQIIKPGRQSIVALLGI 397
BLAST of SMED30025898 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1) HSP 1 Score: 465.307 bits (1196), Expect = 7.770e-163 Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 813 N GMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTES KQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI Sbjct: 358 NTGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESVKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 582 HSP 2 Score: 98.5969 bits (244), Expect = 5.949e-23 Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = 3 Query: 3 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI Sbjct: 313 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 357
BLAST of SMED30025898 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1) HSP 1 Score: 467.233 bits (1201), Expect = 1.699e-162 Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 813 N GMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTES KQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI Sbjct: 434 NTGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESVKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 658 HSP 2 Score: 98.9821 bits (245), Expect = 5.933e-23 Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = 3 Query: 3 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI Sbjct: 389 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 433
BLAST of SMED30025898 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1) HSP 1 Score: 467.233 bits (1201), Expect = 5.447e-162 Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 1 Query: 139 NAGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 813 N GMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTES KQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI Sbjct: 479 NTGMWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESVKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 703 HSP 2 Score: 98.9821 bits (245), Expect = 5.849e-23 Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = 3 Query: 3 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 137 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI Sbjct: 434 SGFLEVIQNSIQTHFLIDAFRRCSYSNYQMENKPGRTALAILMGI 478
BLAST of SMED30025898 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1) HSP 1 Score: 441.81 bits (1135), Expect = 1.866e-156 Identity = 213/215 (99.07%), Postives = 214/215 (99.53%), Query Frame = 1 Query: 169 QIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 813 +IKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTES KQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI Sbjct: 166 RIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESVKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 380
BLAST of SMED30025898 vs. Planmine SMEST
Match: SMESG000051187.1 (SMESG000051187.1) HSP 1 Score: 442.195 bits (1136), Expect = 5.878e-156 Identity = 213/215 (99.07%), Postives = 214/215 (99.53%), Query Frame = 1 Query: 169 QIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESGKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 813 +IKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTES KQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI Sbjct: 211 RIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLSMLYRYHSSACLSDIYSKMYQTEAKRFEIHLRAGNTHKESQIMSEFMKKSTESVKQVSFENNDTKLLEDTSRGQRSKSVGMIPANTIEHVERKMNRRATIKNMEIAHKQIISTELSLQLFKKNLKPKPADQYKTKSREKRMPNIQNMVGKGYFNRKEELYNKTSDTESAYSI 425 The following BLAST results are available for this feature:
BLAST of SMED30025898 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30025898 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30025898 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30025898 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30025898 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30025898 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025898 ID=SMED30025898|Name=SMED30025898|organism=Schmidtea mediterranea sexual|type=transcript|length=867bpback to top protein sequence of SMED30025898-orf-1 >SMED30025898-orf-1 ID=SMED30025898-orf-1|Name=SMED30025898-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp MWFLQSLQIKNADIIYYKDHHIKMYRFLQDSENNVKGLLVRFFLQTTLPLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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