G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30025802
Length (bp)1521
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30025802) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30025802) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30025802) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30025802) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025802

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30025802 SmedASXL_008402SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30025802 dd_Smed_v4_29018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30025802 dd_Smed_v6_29018_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3U400 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS1343 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 4.788e-58
Identity = 142/334 (42.51%), Postives = 208/334 (62.28%), Query Frame = 2 
Query:   62 PKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNG------AXXXXXXXXXXXXQTRLQSPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFS 1045
            P LV  I+ ACIP WS+++ FGL GN LTV +L +  L++ STS YL  LA+ D+LYLL SL  ++ +FI  FP  +R  ++  C+  PFLHYTL+Y+SVWLLV+VTV+R IWVVLPF A+ IC  + A  V V++V VF  +D+HFF T+++        +    +C T  F    FP++DL+ + +LP   M+I N        ++ K R       T   + G + K                 S++LT ML+S NIFF+ S+TPLL+YD+V+F++  +  A+E+ ++   +LFA+E++VYTLWY NF+ HFLLYC+SGP FR E + +F+
Sbjct:   36 PPLVSGIRYACIPVWSILLFFGLFGNALTVFVLRAKRLQQSSTSFYLTVLAITDVLYLLTSLVASIANFIFFFPSEVRQISNTFCVLTPFLHYTLAYISVWLLVAVTVERAIWVVLPFQARNICTKRTAKFVVVTIVSVFTLLDTHFFVTMRYI-----EVQPSYWSCSTTYFADKVFPFLDLLFVAVLPCAIMLIANCLIGWKLRVMRKFRSGVAATSTSNNNQGGSDK--------------REKSTNLTRMLVSTNIFFIVSVTPLLIYDVVFFSVDVRAWAEEDEDVRGGMLFAIERLVYTLWYTNFAIHFLLYCLSGPPFRAEVLVIFN 350
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3WQM9 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS3054 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 4.305e-56
Identity = 143/344 (41.57%), Postives = 213/344 (61.92%), Query Frame = 2 
Query:   14 SGATMNSSDLIPGQEIPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQTRLQSPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFS 1045
            S  T+ + D  P    P L+  I+ ACIP WS+++ FG+ GN LTV +L +  L++ STS YL  LA+ D+LYLL SL  ++ +FI  FP  +R  +   C+  PFL YTL+Y+SVWLLV+VTV+R +WVVLPF A+ IC  + A  V V LV  F  +D HFF T+++     +  +     C+T  F+   FP++DL+++ +LP   M++ N  I +KLR +      R    G     +    + +    K   S +LT ML+S N+FF+ S+TPLL+YD+V+F++  +  A+E  E+   ++FA+E++VYTLWY NF+ HFLLYC+SGP FR E + LF+
Sbjct:   13 SSDTLETKDATPFVT-PPLISGIRYACIPIWSILLCFGIFGNALTVFVLRAKRLQQSSTSFYLTALAITDMLYLLTSLVASIANFIFFFPLELRQLSRTFCVFTPFLSYTLAYISVWLLVTVTVERAVWVVLPFRARHICTKRTAKFVVVLLVFGFTLLDIHFFVTMRY-----HEVRPKYWACMTTYFSDKVFPFLDLLLVAVLPCTIMLVANCLIGWKLRVM------RKFRSGTASGAVSNNNTDQGSGDKREKSINLTRMLVSTNVFFIVSVTPLLIYDVVFFSVDIRAWAEEAEEVRGGMIFAIERLVYTLWYTNFAIHFLLYCLSGPPFRAEVLVLFN 344
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3TQP7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS9870 PE=4 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 7.529e-56
Identity = 152/361 (42.11%), Postives = 220/361 (60.94%), Query Frame = 2 
Query:   29 NSSDLIPGQ--EIPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQTRLQSPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFS---CKGICAQEAPYEAVTEI 1096
             S+D  P    +IP  +  I+   IP W L++ FGL GN LTV +L +  L++ STS YL  LA+ D+LYLL SL  ++ +FI  FP  +R  +   C+ LPF++YTL+Y+SVWLLV+VTV+R IWVVLPF A +IC  + A  V   LV+VF  +D HFF T+++     N +KD    C  ++FT   FPY+DL+++ +LP   M+I N  +I    ++    +T   S    L       +  +  +K   S++LT ML+S N+FF+ S+TPLL+YD+V+F+I  K  A+EN ++   I+F +E+ VYTLWY NF+ HFLLYC+SGP FR E   L +    +   AQ+ P    TEI
Sbjct:   10 TSTDSSPPSAFDIPLSITAIRYTYIPIWILLLFFGLFGNALTVFVLQAKRLKQSSTSFYLTALAITDMLYLLTSLVTSVANFIYFFPFELRQTSKTFCVLLPFINYTLAYISVWLLVAVTVERAIWVVLPFRAHRICTRRTAKFVVPLLVIVFTILDIHFFITMRY----VNISKDKW-ICKPNSFTDKVFPYLDLLLVAVLPCTIMLIAN-CLIGWKLRVMLKFRTGTASASKTLN----SNADPANAEKKEKSTNLTRMLVSTNVFFIISVTPLLIYDVVFFSIDVKAWAEENEKVRGGIIFGLERFVYTLWYTNFAIHFLLYCLSGPPFRAEVFVLLNRIRTRFCNAQKNPIRQKTEI 360
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: U6J6W1 (Growth hormone secretagogue receptor type 1 OS=Echinococcus granulosus OX=6210 GN=EGR_02268 PE=4 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 1.777e-55
Identity = 139/328 (42.38%), Postives = 209/328 (63.72%), Query Frame = 2 
Query:   62 PKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQTRLQSPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFS 1045
            P L+  I+ ACIP WS+++ FG+ GN LTV +L +  L++ STS YL  LA+ D+LYLL SL  ++ +FI  FP  +R  +   C+  PFL YTL+Y+SVWLLV+VTV+R +WVVLPF A+ IC  + A  V + LV+ F  +D HFF T+++        +     C+T  F+   FP++DL+++ ILP   M++ N  I +KLR +      R    G  + ++    + +   +K   S +LT ML+S N+FF+ S+TPLL+YD+V+F++  +  A+E  E+   ++FA+E+ VYTLWY NF+ HFLLYC+SGP FR E + LF+
Sbjct:   28 PPLLSGIRYACIPVWSILLCFGIFGNALTVFVLRTKRLQQSSTSFYLTALAITDMLYLLTSLVASIANFIFFFPSELRQLSRTFCVFTPFLSYTLAYISVWLLVTVTVERAVWVVLPFRARHICTKRTAKSVVLLLVIGFTLLDIHFFLTMRY-----QEVRPGYWLCMTTYFSDKVFPFLDLLLVAILPCTIMLVANCLIGWKLRVM------RKFRSGTAIGMVSNNITDQGGGEKREKSVNLTRMLVSTNVFFIVSVTPLLIYDVVFFSVDVRAWAEEAEEVRGGMIFAIERFVYTLWYTNFAIHFLLYCLSGPPFRAEVLVLFN 344
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A068Y4I9 (Growth hormone secretagogue receptor type 1 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000433200 PE=4 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 3.166e-55
Identity = 139/328 (42.38%), Postives = 208/328 (63.41%), Query Frame = 2 
Query:   62 PKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQTRLQSPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFS 1045
            P L+  I+ ACIP WS+++ FG+ GN LTV +L +  L++ STS YL  LA+ D+LYLL SL  ++ +FI  FP  +R  +   C+  PFL YTL+Y+SVWLLV+VTV+R +WVVLPF A+ IC  + A  V + LV+ F  +D HFF T+++              C+T  F+   FP++DL+++ ILP   M++ N  I +KLR +      R    G  + ++    + +   +K   S +LT ML+S N+FF+ S+TPLL+YD+V+F++  +  A+E  E+   ++FA+E+ VYTLWY NF+ HFLLYC+SGP FR E + LF+
Sbjct:   28 PPLLSGIRYACIPVWSILLCFGIFGNALTVFVLRTKRLQQSSTSFYLTALAITDMLYLLTSLVASIANFIFFFPSELRQLSRTFCVFTPFLSYTLAYISVWLLVTVTVERAVWVVLPFRARHICTKRTAKFVVLLLVIGFTLLDIHFFLTMRY-----QEVGPGYWLCMTTYFSDKVFPFLDLLLVAILPCAIMLVANCLIGWKLRVM------RKFRSGTAIGMVSNNNTDQGGGEKREKSVNLTRMLVSTNVFFIVSVTPLLIYDVVFFSVDVRAWAEEAEEVRGGMIFAIERFVYTLWYTNFAIHFLLYCLSGPPFRAEVLVLFN 344
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000002498.1 (SMESG000002498.1)

HSP 1 Score: 197.978 bits (502), Expect = 1.374e-57
Identity = 145/358 (40.50%), Postives = 218/358 (60.89%), Query Frame = 2 
Query:   62 PKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQ------------------------TRLQSPGDNLKVL--EPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRA--KENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFSCK 1051
            P  +  I+N CI  W++I+ FGL GN LTVA+L SP L   ST+ YL  LA++DILYL+ SL   + +F   FP ++R K    C+ L  + +TL+Y+SVWLLV+V +DRMIWV+ PF AK IC  K A I+ +SL+ + + ID HF++TLKF +      K   N+C      ++ F YIDL +++ LP   M + N  II KLR+++K+R+                         R  +  +N++ +  E  +    ++ +   ++SLT MLLS+NIFF+ S TPLL Y+I+++  +P I++   +    +  ++F +E+++YTLWYLNF+ HFLLYCMSGP+FR EAI+L  CK
Sbjct:   16 PPAIDIIRNICIGVWAIIVLFGLFGNCLTVAVLISPKLNNSSTTFYLTVLALIDILYLIFSLGGQMLNFSIFFPAALRQKNMFACISLSMIRFTLTYISVWLLVAVAIDRMIWVIFPFKAKMICTRKIAKIIVISLIAMLVLIDLHFYWTLKFTY-----NKGSVNSCELKESYRNPFLYIDLTLVMGLPFFVMSVANSLIISKLREMEKLRKGMKSTNRIPNDSVIPNLNATSKFSNRKHAKNNNVEEITCEIVKRSTRRRSRDKQTTSLTKMLLSVNIFFLVSTTPLLSYNILFY--VPHIKSLLLQFENQLTGLIFGIERIIYTLWYLNFAVHFLLYCMSGPKFRQEAIRLL-CK 365
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000060945.1 (SMESG000060945.1)

HSP 1 Score: 185.267 bits (469), Expect = 9.932e-54
Identity = 148/340 (43.53%), Postives = 213/340 (62.65%), Query Frame = 2 
Query:   53 QEIPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYXXXXXXXXXXXXXXXXCNGAXXXXXXXXXXXXQTRLQ-------SPGDNLKVLEPXXXXXXXXXXXXXXXXLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFSCK 1051
            +  P+ V  I+  CIP W++II  GLVGN+LT+ +L   +++  ST+VYL  LAVVD+LYL+CS  V+L  +I  FP S+R  ++ILC  +P L Y+LS+ S WL+V+VT DRMI VVLPF +KK  N   A IV ++++++ I+++ HF +T+ F           T  C    FT+  FPY+DL ++ I+P + M+ CN AII KLR   KIR+  L+       +PG +    E        K     + +LT MLL  N+FF+ S+TPLLVYDIVYF I  +   KEN ++   +LF +E++VYT+WYLNF  HF +YC+SGP FR+ A+K+   K
Sbjct:   15 ESTPQSVQIIRFLCIPIWAIIIIVGLVGNILTIKVLRFRHIKSTSTAVYLSALAVVDLLYLICSTNVSLIDYIFFFPSSVRHTSAILCHVVPMLQYSLSHFSAWLVVAVTFDRMILVVLPFSSKKFSNENMALIVIITILIIIISVNIHFLWTMTF-----------TTACNGLYFTKTVFPYVDLALMGIVPFMAMICCNLAIILKLRAQTKIREKNLKKTTKSPLNPGTSNNHDEK----PFVKNFKDKAGNLTKMLLCTNLFFMLSMTPLLVYDIVYFLIDAQALLKENEQLWGGLLFGIERLVYTIWYLNFCIHFFVYCLSGPNFRDAAMKILKLK 339
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000047150.1 (SMESG000047150.1)

HSP 1 Score: 109.383 bits (272), Expect = 3.540e-26
Identity = 72/154 (46.75%), Postives = 100/154 (64.94%), Query Frame = 2 
Query:   59 IPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKF 520
            +P  V  I+  CI  W+ II  G+ GN+LTV +L+ P L   ST+ YL  LA+VD+LYL+ SL   +  F  +FP+++R    + C  L  + YTL+Y+SVWLLV+V++DRMIWV+LPF AK IC  K A I+     + FI ID HF +T+KF
Sbjct:   15 MPDSVEIIRRICIAVWANIILVGVFGNILTVIVLAGPKLNTTSTTFYLTILAIVDLLYLIFSLGGQMVDFALLFPNTMRQSNELSCKLLNIILYTLAYISVWLLVAVSIDRMIWVLLPFKAKLICTRKVAKIIVSIFCIFFIGIDIHFIWTVKF 168
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000047150.1 (SMESG000047150.1)

HSP 1 Score: 108.997 bits (271), Expect = 5.795e-26
Identity = 72/154 (46.75%), Postives = 100/154 (64.94%), Query Frame = 2 
Query:   59 IPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKF 520
            +P  V  I+  CI  W+ II  G+ GN+LTV +L+ P L   ST+ YL  LA+VD+LYL+ SL   +  F  +FP+++R    + C  L  + YTL+Y+SVWLLV+V++DRMIWV+LPF AK IC  K A I+     + FI ID HF +T+KF
Sbjct:   15 MPDSVEIIRRICIAVWANIILVGVFGNILTVIVLAGPKLNTTSTTFYLTILAIVDLLYLIFSLGGQMVDFALLFPNTMRQSNELSCKLLNIILYTLAYISVWLLVAVSIDRMIWVLLPFKAKLICTRKVAKIIVSIFCIFFIGIDIHFIWTVKF 168

HSP 2 Score: 52.7582 bits (125), Expect = 3.629e-7
Identity = 26/55 (47.27%), Postives = 39/55 (70.91%), Query Frame = 2 
Query:  794 LTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYT 958
            LT MLLS+N+FF+AS  PLL+YD++Y+ I  K   ++    I  ++F VE++VYT
Sbjct:  293 LTRMLLSVNLFFMASTAPLLIYDVIYYLIDFKEMIQKYGIYIGGVIFGVERLVYT 347
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000037421.1 (SMESG000037421.1)

HSP 1 Score: 108.612 bits (270), Expect = 2.598e-25
Identity = 72/154 (46.75%), Postives = 100/154 (64.94%), Query Frame = 2 
Query:   59 IPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNLRRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGLPFLHXXXXXXXXXXXXXXXXDRMIWVVLPFHAKKICNHKNAFIVAVSLVLVFIAIDSHFFFTLKF 520
            +P  V  I+  CI  W+ II  G+ GN+LTV +L+ P L   ST+ YL  LA+VD+LYL+ SL   +  F  +FP+++R    + C  L  + YTL+Y+SVWLLV+V++DRMIWV+LPF AK IC  K A I+     + FI ID HF +T+KF
Sbjct:   15 MPDSVEIIRRICIAVWANIILVGVFGNILTVIVLAGPKLNTTSTTFYLTILAIVDLLYLIFSLGGQMVDFALLFPNTMRQSNELSCKLLNIILYTLAYISVWLLVAVSIDRMIWVLLPFKAKLICTRKVAKIIVSIFCIFFIGIDIHFIWTVKF 168

HSP 2 Score: 100.138 bits (248), Expect = 2.000e-22
Identity = 48/83 (57.83%), Postives = 63/83 (75.90%), Query Frame = 2 
Query:  794 LTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLF 1042
            LT MLLS+N+FF+AS  PLL+YD++Y+ I  K   ++    I  ++F VE++VYT WYLNFS HFLLYC+SGP+FR EA KLF
Sbjct:  293 LTRMLLSVNLFFMASTAPLLIYDVIYYLIDFKEMIQKYGIYIGGVIFGVERLVYTFWYLNFSIHFLLYCLSGPKFREEA-KLF 374
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3U4004.788e-5842.51G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3WQM94.305e-5641.57G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3TQP77.529e-5642.11G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
U6J6W11.777e-5542.38Growth hormone secretagogue receptor type 1 OS=Ech... [more]
A0A068Y4I93.166e-5542.38Growth hormone secretagogue receptor type 1 OS=Ech... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002498.11.374e-5740.50SMESG000002498.1[more]
SMESG000060945.19.932e-5443.53SMESG000060945.1[more]
SMESG000047150.13.540e-2646.75SMESG000047150.1[more]
SMESG000047150.15.795e-2646.75SMESG000047150.1[more]
SMESG000037421.12.598e-2546.75SMESG000037421.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025802 ID=SMED30025802|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1521bp
CAGTTTTTCCTAATCTGGTGCGACGATGAACAGCAGTGATTTAATTCCAG
GTCAAGAAATACCTAAACTTGTCCTTTATATCAAAAATGCTTGTATTCCT
ACATGGAGTTTAATTATAGCTTTCGGATTAGTTGGGAATGTTCTCACGGT
GGCTCTTTTGAGTAGCCCAAATTTACGTCGACACAGTACGTCAGTGTATC
TCCGTTGGTTGGCGGTTGTTGACATCCTTTATCTGCTATGCAGTTTGACG
GTAAATCTGTTCTCATTCATCACAGTATTTCCCGATTCCATTCGTGACAA
AGCATCCATTTTGTGCCTGGGTCTTCCTTTTCTACATTACACCCTCAGCT
ATCTCAGTGTATGGCTTCTAGTATCTGTTACTGTCGACAGAATGATTTGG
GTCGTTCTTCCATTTCACGCGAAAAAGATATGCAATCACAAAAATGCATT
CATTGTCGCCGTCTCATTGGTACTGGTATTCATTGCCATTGACAGTCATT
TCTTTTTCACTTTGAAATTCGATTTCAAATCATCAAATTCAACCAAGGAT
CCAACAAATACGTGCGTTACGGATACATTCACTCAGGATGCATTTCCTTA
CATAGATTTGGTCATCCTTCTAATTCTGCCACTGATCTTTATGGTGATAT
GCAATGGAGCAATCATTTTTAAACTGCGGAAACTGAAAAAGATAAGACAA
ACTCGTCTTCAATCTCCAGGTGATAATTTGAAGGTTCTGGAGCCGGGTCA
AAGCATAAAGAGCAAGAAGCAGAAATCCATGGGATCGTCGAGTTTAACGG
CGATGCTGTTGAGCATAAATATATTTTTTGTAGCATCAATAACCCCCCTT
CTTGTGTATGACATTGTCTACTTCGCCATTCTACCGAAAATCAGAGCAAA
GGAAAATGCGGAAATGATTTATCAAATACTTTTTGCCGTTGAACAAGTCG
TTTATACTCTTTGGTACCTCAACTTTTCTGCCCACTTTCTACTTTATTGC
ATGAGTGGTCCGAGATTTCGAAATGAAGCCATTAAATTATTCAGCTGCAA
AGGAATTTGTGCCCAAGAAGCTCCATACGAAGCAGTAACAGAGATCATGA
CAAGGAGGATGAGTTCACACTATCGGCATTAATAGGATGTTCCACATGGG
AAAATCCCAATTGAATTGGTTCATATCCATACCAGCCTTACAAGCTCTTA
CCAAATATGTCTTTCACAAATAATATTTGAAAGGTAAGTCATTCGAATCG
AATGAGTAAATATTTGTCAGTCTTTATAAAGATCCTGTGATACCCCCTAG
CCAACTATGCCATGAATCCTCCAGATACTCGCTGTGCGTGACCTAATCAC
AGTCCCAGTGACTCTAGACTATGATTTCTCTTCAAGTTCAGTTCTAGTTT
GAAGTGTCATCGGTTCTCAATGAGATTCTCTGTCATCCAAAATCCTTTTG
AATCCTTGGTCAATTGAGTTCCTGGATTGGATTAATTTTTCCTAGATTAT
ATTGAAAGGATTATAACCTCA
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protein sequence of SMED30025802-orf-1

>SMED30025802-orf-1 ID=SMED30025802-orf-1|Name=SMED30025802-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=369bp
MNSSDLIPGQEIPKLVLYIKNACIPTWSLIIAFGLVGNVLTVALLSSPNL
RRHSTSVYLRWLAVVDILYLLCSLTVNLFSFITVFPDSIRDKASILCLGL
PFLHYTLSYLSVWLLVSVTVDRMIWVVLPFHAKKICNHKNAFIVAVSLVL
VFIAIDSHFFFTLKFDFKSSNSTKDPTNTCVTDTFTQDAFPYIDLVILLI
LPLIFMVICNGAIIFKLRKLKKIRQTRLQSPGDNLKVLEPGQSIKSKKQK
SMGSSSLTAMLLSINIFFVASITPLLVYDIVYFAILPKIRAKENAEMIYQ
ILFAVEQVVYTLWYLNFSAHFLLYCMSGPRFRNEAIKLFSCKGICAQEAP
YEAVTEIMTRRMSSHYRH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 306..332
score: 21.66
coord: 22..46
score: 24.09
coord: 55..76
score: 27.14
coord: 263..286
score: 22.78
coord: 104..126
score: 23.49
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 37..304
e-value: 4.2E-18
score: 65.6
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 17..347
e-value: 3.1E-48
score: 166.4
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 29..347
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 29..347
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 29..335
e-value: 5.13968E-35
score: 128.134
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 26..348
NoneNo IPR availableTMHMMTMhelixcoord: 61..83
NoneNo IPR availableTMHMMTMhelixcoord: 98..120
NoneNo IPR availableTMHMMTMhelixcoord: 195..217
NoneNo IPR availableTMHMMTMhelixcoord: 264..286
NoneNo IPR availableTMHMMTMhelixcoord: 301..323
NoneNo IPR availableTMHMMTMhelixcoord: 26..48
NoneNo IPR availableTMHMMTMhelixcoord: 141..163
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 37..324
score: 25.768