SMED30025734

Overview
NameSMED30025734
Smed IDSMED30025734
Length (bp)2129
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025734 (SMED30025734) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025734 (SMED30025734) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025734 (SMED30025734) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025734 (SMED30025734) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025734

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30025734h1SMcG0019625 dd_Smed_v4_6553_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025734h1SMcG0019625 dd_Smed_v4_6553_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
whole organismSMED30025734h1SMcG0019625 Contig54263newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30025734h1SMcG0019625 Contig54263uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30025734h1SMcG0019625 Contig41790uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30025734h1SMcG0019625 Contig41790newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025734 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001002:42384..45820 -10341
Homology
[Close]
BLAST of SMED30025734 vs. Planmine SMEST
Match: SMESG000014783.1 (SMESG000014783.1)

HSP 1 Score: 1285.4 bits (3325), Expect = 0.000e+0
Identity = 649/664 (97.74%), Postives = 654/664 (98.49%), Query Frame = 2 
Query:   83 MLSVRLIIFIFYLELVGKXXXXXXXXXXXXHLLNTSFQLQFPIHLPYEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKDTIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMKEYQAAQSKYFANLIIKDIKEHINRLILKSGSFINKNESL-GVATRSDKLTSQIESLVKENLKKIYRNITNQINRWTTQVNKSNGRCVQDSLEKLNLIVNLTQQNIREHKSVNVTQMNEIILIQNKIEQLKKTISLLKETQPNRLKELKENYERFNSLADLMIRNLSKVHLNIKNELKVNRKNDKKGFNENIKSDRLLFWSIDGILRYVRTGKSLFAHKNKFENIYDIYVWKFFDPDLFRSHLTFRVFPFVALPRRMRVTIQLVNSDNDIYVSTAKEIVIASRFIKSPDFKFHYEDFTNFHKNELDSVIMKCHFLWI 2071
            MLSVRLIIFIFYLELVGKVFPFPTVRPTFPHLLNTSFQLQFPIH  YEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKDTIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDN+SSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMKEYQ  QSKYFANLIIKDIKEHINRLILKSGSFINKNESL GVATRSDKLTSQIESLVKE LK IYRNITNQINRWTTQVNKSNGRCVQDSLEKLNLIVNLTQQNIREHKSVNVTQMNEIILIQNKIEQLKK+ISLLK+TQPNRLKELKENYERFNSLADLMIRNLSKVH NIKNELKVNRKNDKKGF+ENIKSDRLLFWSIDGILRYVRTGKSLFAHKNKFENIYDIYVWKFFDPDLFRSHLTF+VF FVALPRRMRVTIQLVN DNDIYVSTAKEIVIASRFIKSPDFKFHYEDFTNFHKNELDSVIMKCHFLWI
Sbjct:    1 MLSVRLIIFIFYLELVGKVFPFPTVRPTFPHLLNTSFQLQFPIHPQYEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKDTIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNISSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMKEYQTTQSKYFANLIIKDIKEHINRLILKSGSFINKNESLEGVATRSDKLTSQIESLVKETLKIIYRNITNQINRWTTQVNKSNGRCVQDSLEKLNLIVNLTQQNIREHKSVNVTQMNEIILIQNKIEQLKKSISLLKKTQPNRLKELKENYERFNSLADLMIRNLSKVHQNIKNELKVNRKNDKKGFSENIKSDRLLFWSIDGILRYVRTGKSLFAHKNKFENIYDIYVWKFFDPDLFRSHLTFKVFAFVALPRRMRVTIQLVNGDNDIYVSTAKEIVIASRFIKSPDFKFHYEDFTNFHKNELDSVIMKCHFLWI 664
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BLAST of SMED30025734 vs. Planmine SMEST
Match: SMESG000069999.1 (SMESG000069999.1)

HSP 1 Score: 196.823 bits (499), Expect = 2.903e-54
Identity = 113/319 (35.42%), Postives = 172/319 (53.92%), Query Frame = 2 
Query:  179 LNTSFQLQFPIHLPYEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKD--TIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMKE 1129
            LN+ F++Q   +L    V   + DTM+I+IT+    + +R IF +G  DTI++E KG YM+I   EV+ +  +I + I F + S+  + EIY + A +K+  KK +KNL+L+++IYK  I L+ P  L   + S  G  +  E+N    A CRA+DG P E    E  + F      F   +NE  I      E H   L+C+  +     +I+V H PT+L I P++ K +  N    CYAKDGFP   ++W L++GP N SN+Y +      +  K N P+ S W+F C   +   NS   L K ISFF E +++ +
Sbjct:   37 LNSIFEIQIQPNLSEPTVAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNPLQCRAGSEVKVHTIKVFHHPTELFIKPKL-KLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQ 354
[Close]
BLAST of SMED30025734 vs. Planmine SMEST
Match: SMESG000014496.1 (SMESG000014496.1)

HSP 1 Score: 193.356 bits (490), Expect = 3.564e-53
Identity = 112/320 (35.00%), Postives = 168/320 (52.50%), Query Frame = 2 
Query:  173 HLLNTSFQLQFPIHLPYEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKDTIRV--SIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMK 1126
            HLL  +F +Q         ++   +DTMVIRI + +    E+ I N+G  D ID+E  GK+MDI + E +  ++R+  SI FKR +H + +EI+  SA  K +R+K LKNL L++++    I L++P +L L +   N     + +N +  A+CRA+ G PQE    E        +K ++   NE ++   AS   H     C   NV+ + +I++ H PT L I P I K    N  I C+ KDG+P   I W LI+GP N+  FY       QI    N P  S W F C+ +    N+  +LNK ISF I ++ M+
Sbjct:   33 HLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFQRRKYLKNLSLAKRLLYQKIELFMPSQLNLSMYPLNVDRKEIIINDMVMASCRAFGGIPQEKPKFEDHVHLNVMEKIYNKTTNEIRMNLRASLSDHLTNFSCSAGNVTETYTIRIHHHPTALYIQPIIKKRFRSNSTIICFTKDGYPDPEINWQLIEGPPNADRFYNFPGKGDQIHINSNSPVGSKWTFKCTMKSMLVNTKKMLNKTISFAIAKNPME 352
[Close]
BLAST of SMED30025734 vs. Planmine SMEST
Match: SMESG000070072.1 (SMESG000070072.1)

HSP 1 Score: 182.185 bits (461), Expect = 1.329e-49
Identity = 115/348 (33.05%), Postives = 177/348 (50.86%), Query Frame = 2 
Query:   98 LIIFIFYLELV---GKXXXXXXXXXXXXHLLNTSFQLQFPIHLPYEPVTKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKD--TIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMK 1126
            L ++ F+L L+    ++  F      FPHL+  +F L          ++   +DTM IRI + +   +ER    +G L+ +DDEFKGK MD+ + E +     + +SI F R +H + +EI+  SA    ERKK LKNL L+++++  +I L++P  L L +         + +N +  A+C+A+ G PQE    E       T+  ++   NE ++   AS   H     C   NV+ + +I++ H PT L I P I K  + N  I CY  DG+P   I W LI+GP N++ FY I     QI    N  + S W F C+ +    N+  ILNK ISF I  + M+
Sbjct:    4 LTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNRAAHNKYLEIFYFSASNALERKKYLKNLSLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPME 350
[Close]
BLAST of SMED30025734 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 171.4 bits (433), Expect = 3.060e-47
Identity = 98/290 (33.79%), Postives = 154/290 (53.10%), Query Frame = 2 
Query:  245 TDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVDKDT--IRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVIALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKIFKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPTDLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDSTNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFI 1108
            +DTMVIR+ + +   +ER    +G L  +DDEFKG  MDI + E ++ +  I +SI FKR +H + +EI+  SA  K  R+K LKNL L+++++  +I L++P  L L L         + +N +  A+CRA+ G PQE    E       T+  ++   NE  +    S + H     C   NV+ +  I + H P+ + I P I   ++ N  I CYA+DG+P   + + LIKGP N+S++Y       +I  + N P+RS+W F C      +   +   KNI+F I
Sbjct:   56 SDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKDIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKCLMISTIAGVAMTKEKNINFRI 345
The following BLAST results are available for this feature:
BLAST of SMED30025734 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025734 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014783.10.000e+097.74SMESG000014783.1[more]
SMESG000069999.12.903e-5435.42SMESG000069999.1[more]
SMESG000014496.13.564e-5335.00SMESG000014496.1[more]
SMESG000070072.11.329e-4933.05SMESG000070072.1[more]
SMESG000014554.13.060e-4733.79SMESG000014554.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025734 ID=SMED30025734|Name=SMED30025734|organism=Schmidtea mediterranea sexual|type=transcript|length=2129bp
CGCGTATCAATCCAGTTCAAATATAAACTGATGAAAATGACTAATTAATT
AAATTAAAATTACAAATTTAACGTTTCCTGAAATGTTGTCAGTGAGATTA
ATTATTTTCATATTTTACCTGGAATTGGTCGGAAAGGTCTTTCCGTTTCC
CACAGTTCGGCCAACGTTTCCACATCTATTGAATACGTCTTTTCAGTTAC
AGTTTCCAATTCATCTGCCATATGAACCTGTGACCAAACACATCACAGAT
ACGATGGTAATACGCATTACGAATAAGTCCGAAGCTGTCCTCGAACGACA
TATTTTCAATCATGGATATTTAGACACCATAGATGATGAATTCAAAGGCA
AATATATGGATATACCCTTATTGGAGGTGGACAAAGATACAATACGAGTT
TCTATTCATTTTAAACGAATATCTCACGGTCAAATAATTGAGATCTATCT
GCTTTCAGCACAAACAAAGCAAGAACGAAAGAAAATGCTGAAAAATCTAA
AATTATCAAGACAAATTTATAAATCAGTTATTGCGTTATATGTTCCTTAC
AGATTGGAGTTGAAGCTGGGTTCTGCAAACGGAACTACGAATCCTCTTGA
GCTCAACCGATTGGCGGCCGCCACTTGTAGGGCTTACGATGGATTTCCGC
AGGAAAATGTCACAATTGAAATTCCAAAAATTTTCAAGGAAACTAAAAAA
GAATTTGATTTCAATAAAAATGAATACAAAATTTGGTTCAATGCTTCAAA
AGAAATTCATAATAAAATACTAAAATGCAAGGTTGATAACGTCTCCAGTT
CCGTGTCAATTCAGGTCTTACATCCTCCAACGGATCTTTTAATCTCTCCT
CAAATAAATAAATCCATTGAAAAAAATGTTGTCATTTCATGTTATGCGAA
AGATGGTTTTCCTGCCAGTCGTATCAAGTGGCATTTGATTAAAGGTCCCG
AAAACTCCTCAAATTTTTATATAATTGATTCAACAAATTATCAGATTCGT
TTCAAAGTAAATGTGCCAAGCAGATCTTCGTGGGAATTCAACTGCTCTTC
AGAATACAATACTTCAAATTCTTTAATCATTTTAAACAAAAATATTTCTT
TTTTCATTGAAGAAGACTCGATGAAGGAGTATCAGGCTGCTCAGTCCAAA
TACTTTGCAAATCTAATAATAAAGGATATTAAAGAGCACATTAACAGATT
AATTTTGAAGTCGGGGTCCTTCATAAACAAGAATGAGAGTCTAGGTGTGG
CAACACGATCAGATAAACTAACTAGTCAAATTGAATCCTTAGTGAAAGAA
AATCTAAAGAAAATCTATCGGAATATCACCAACCAAATAAATAGATGGAC
TACCCAAGTGAATAAAAGCAACGGGAGATGTGTCCAGGACTCCTTAGAGA
AGCTAAACCTTATTGTCAACCTCACCCAACAAAACATCAGAGAACACAAA
TCAGTGAATGTTACTCAAATGAATGAAATAATTCTAATTCAAAACAAAAT
AGAACAACTTAAGAAGACCATCAGCTTATTGAAAGAAACCCAACCAAATA
GATTAAAGGAATTGAAAGAGAATTATGAAAGATTCAATTCCTTGGCAGAT
TTGATGATTAGAAACCTGTCTAAGGTTCATCTGAATATTAAAAACGAATT
GAAAGTAAATCGAAAGAACGACAAGAAAGGTTTCAACGAGAACATCAAAT
CCGACCGTTTGCTATTCTGGTCCATTGATGGAATTCTTCGCTATGTCCGA
ACTGGGAAAAGTTTATTTGCTCACAAGAATAAATTTGAAAATATTTACGA
TATCTACGTTTGGAAATTTTTCGACCCCGACCTATTTCGAAGCCATCTGA
CTTTTAGGGTGTTTCCTTTCGTGGCGCTGCCCCGCCGTATGCGAGTGACT
ATCCAGCTAGTCAACAGTGACAATGACATCTACGTCTCAACCGCCAAGGA
AATCGTGATCGCTAGCCGATTTATAAAATCTCCGGACTTTAAATTCCATT
ATGAAGATTTTACAAATTTTCATAAAAATGAACTCGATTCCGTGATAATG
AAATGTCACTTTTTATGGATATAAATCTATGACTTTTGCTTGTCATTTGG
GAGAATAAATATTTGTATCAGAAAAAAAA
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protein sequence of SMED30025734-orf-1

>SMED30025734-orf-1 ID=SMED30025734-orf-1|Name=SMED30025734-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=664bp
MLSVRLIIFIFYLELVGKVFPFPTVRPTFPHLLNTSFQLQFPIHLPYEPV
TKHITDTMVIRITNKSEAVLERHIFNHGYLDTIDDEFKGKYMDIPLLEVD
KDTIRVSIHFKRISHGQIIEIYLLSAQTKQERKKMLKNLKLSRQIYKSVI
ALYVPYRLELKLGSANGTTNPLELNRLAAATCRAYDGFPQENVTIEIPKI
FKETKKEFDFNKNEYKIWFNASKEIHNKILKCKVDNVSSSVSIQVLHPPT
DLLISPQINKSIEKNVVISCYAKDGFPASRIKWHLIKGPENSSNFYIIDS
TNYQIRFKVNVPSRSSWEFNCSSEYNTSNSLIILNKNISFFIEEDSMKEY
QAAQSKYFANLIIKDIKEHINRLILKSGSFINKNESLGVATRSDKLTSQI
ESLVKENLKKIYRNITNQINRWTTQVNKSNGRCVQDSLEKLNLIVNLTQQ
NIREHKSVNVTQMNEIILIQNKIEQLKKTISLLKETQPNRLKELKENYER
FNSLADLMIRNLSKVHLNIKNELKVNRKNDKKGFNENIKSDRLLFWSIDG
ILRYVRTGKSLFAHKNKFENIYDIYVWKFFDPDLFRSHLTFRVFPFVALP
RRMRVTIQLVNSDNDIYVSTAKEIVIASRFIKSPDFKFHYEDFTNFHKNE
LDSVIMKCHFLWI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000136whole organism
PLANA:0002142parenchyma
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000551MerR-type_HTH_dom
IPR007110Ig-like_dom
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 492..512
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.696
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 248..339
score: 7.123