Splicing factor 3B subunit 5

Overview
NameSplicing factor 3B subunit 5
Smed IDSMED30025731
Length (bp)247
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Splicing factor 3B subunit 5 (SMED30025731) t-SNE clustered cells

Violin plots show distribution of expression levels for Splicing factor 3B subunit 5 (SMED30025731) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Splicing factor 3B subunit 5 (SMED30025731) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Splicing factor 3B subunit 5 (SMED30025731) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025731

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30025731SMESG000014121.1 SmedASXL_002192SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30025731SMESG000014121.1 dd_Smed_v4_2023_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025731SMESG000014121.1 dd_Smed_v4_2023_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30025731SMESG000014121.1 dd_Smed_v4_2023_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025731SMESG000014121.1 dd_Smed_v4_2023_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30025731SMESG000014121.1 dd_Smed_v4_2023_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Human
Match: SF3B5 (splicing factor 3b subunit 5 [Source:HGNC Symbol;Acc:HGNC:21083])

HSP 1 Score: 130.954 bits (328), Expect = 3.320e-41
Identity = 55/79 (69.62%), Postives = 66/79 (83.54%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Fly
Match: Sf3b5 (gene:FBgn0040534 transcript:FBtr0082006)

HSP 1 Score: 133.265 bits (334), Expect = 1.962e-42
Identity = 56/79 (70.89%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MG+RYNIH+QLEHLQSKY+G GH+DTTK+EW+TN HRD+ AS +GH D L+  AI ENE+KARV+ NLME+M+QPCGPP
Sbjct:    1 MGERYNIHSQLEHLQSKYIGTGHADTTKFEWLTNQHRDSLASYMGHYDILNYFAIAENESKARVRFNLMERMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Zebrafish
Match: sf3b5 (splicing factor 3b, subunit 5 [Source:ZFIN;Acc:ZDB-GENE-040718-181])

HSP 1 Score: 131.724 bits (330), Expect = 1.275e-41
Identity = 55/79 (69.62%), Postives = 67/79 (84.81%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRYNIH+QLEHLQSKY+G GH+DT+KWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYNIHSQLEHLQSKYIGTGHADTSKWEWLVNQHRDSYCSYMGHFDLLNYFAISENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Xenopus
Match: sf3b5 (splicing factor 3b subunit 5 [Source:NCBI gene;Acc:549195])

HSP 1 Score: 130.568 bits (327), Expect = 4.668e-41
Identity = 54/79 (68.35%), Postives = 66/79 (83.54%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  A+ ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAVAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Mouse
Match: Sf3b5 (splicing factor 3b, subunit 5 [Source:MGI Symbol;Acc:MGI:1913375])

HSP 1 Score: 127.487 bits (319), Expect = 7.230e-40
Identity = 54/79 (68.35%), Postives = 65/79 (82.28%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QP GPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPSGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Match: sp|Q9VHI4|SF3B5_DROME (Splicing factor 3B subunit 5 OS=Drosophila melanogaster OX=7227 GN=Sf3b5 PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.967e-41
Identity = 56/79 (70.89%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MG+RYNIH+QLEHLQSKY+G GH+DTTK+EW+TN HRD+ AS +GH D L+  AI ENE+KARV+ NLME+M+QPCGPP
Sbjct:    1 MGERYNIHSQLEHLQSKYIGTGHADTTKFEWLTNQHRDSLASYMGHYDILNYFAIAENESKARVRFNLMERMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Match: sp|Q9BWJ5|SF3B5_HUMAN (Splicing factor 3B subunit 5 OS=Homo sapiens OX=9606 GN=SF3B5 PE=1 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.594e-40
Identity = 55/79 (69.62%), Postives = 66/79 (83.54%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Match: sp|Q56K13|SF3B5_BOVIN (Splicing factor 3B subunit 5 OS=Bos taurus OX=9913 GN=SF3B5 PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.594e-40
Identity = 55/79 (69.62%), Postives = 66/79 (83.54%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Match: sp|Q923D4|SF3B5_MOUSE (Splicing factor 3B subunit 5 OS=Mus musculus OX=10090 GN=Sf3b5 PE=1 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 5.057e-39
Identity = 54/79 (68.35%), Postives = 65/79 (82.28%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRY IH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  AI ENE+KARV+ NLMEKM+QP GPP
Sbjct:    1 MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPSGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Match: sp|Q55BF5|SF3B5_DICDI (Splicing factor 3B subunit 5 OS=Dictyostelium discoideum OX=44689 GN=sf3b5 PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.042e-30
Identity = 44/78 (56.41%), Postives = 60/78 (76.92%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGP 240
            M +R ++++QLEHLQ +YVG GH+D +K EW+TN HRD+ +S IGH+ FLSL +I ENE+  RV+ N + KMI PCGP
Sbjct:    1 MSERESLNSQLEHLQMRYVGTGHADISKHEWLTNQHRDSLSSFIGHSSFLSLFSIAENESVGRVRYNTLTKMISPCGP 78
[Close]
BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Match: A0A3Q0KMI8 (Splicing factor 3B subunit 5 OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.811e-41
Identity = 61/79 (77.22%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MGDRYNIHTQLEHLQSKYVG GH+DTTKWEW+TN HRD+ AS +GH D L+L AI ENE KARV+ NL+EKM+QPCGPP
Sbjct:    1 MGDRYNIHTQLEHLQSKYVGTGHADTTKWEWLTNQHRDSCASYLGHFDMLNLFAICENECKARVRFNLLEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Match: A0A336LKL3 (Splicing factor 3B subunit 5 OS=Culicoides sonorensis OX=179676 GN=CSON010528 PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 4.176e-41
Identity = 61/79 (77.22%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MGDRYNIH+QLEHLQSKY+G GH+DTTK+EW+TN HRD+ AS IG  D LS  AIVENETKARVK NLMEK++QPCGPP
Sbjct:    1 MGDRYNIHSQLEHLQSKYIGTGHADTTKYEWLTNQHRDSLASYIGRYDMLSYFAIVENETKARVKFNLMEKLLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Match: C1LKP9 (Splicing factor 3B subunit 5 OS=Schistosoma japonicum OX=6182 GN=SF3b5 PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 4.506e-41
Identity = 60/79 (75.95%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MGDRYNIHTQLEHLQSKYVG GH+DTTKWEW+TN HRD+ AS +GH D L+L A+ ENE KARV+ NL+EKM+QPCGPP
Sbjct:    1 MGDRYNIHTQLEHLQSKYVGTGHADTTKWEWLTNQHRDSCASYLGHFDMLNLFAVCENECKARVRFNLLEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Match: A0A430QJ55 (Splicing factor 3B subunit 5 OS=Schistosoma bovis OX=6184 GN=DC041_0008549 PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 6.061e-41
Identity = 61/79 (77.22%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MGDRYNIHTQLEHLQSKYVG GH+DTTKWEW+TN HRD+ AS +GH D L+L AI ENE KARV+ NL+EKM+QPCGPP
Sbjct:    1 MGDRYNIHTQLEHLQSKYVGTGHADTTKWEWLTNQHRDSCASYLGHFDTLNLFAICENECKARVRFNLLEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Match: A0A183K6M3 (Splicing factor 3B subunit 5 OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS10648 PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 6.061e-41
Identity = 61/79 (77.22%), Postives = 69/79 (87.34%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            MGDRYNIHTQLEHLQSKYVG GH+DTTKWEW+TN HRD+ AS +GH D L+L AI ENE KARV+ NL+EKM+QPCGPP
Sbjct:    1 MGDRYNIHTQLEHLQSKYVGTGHADTTKWEWLTNQHRDSCASYLGHFDTLNLFAICENECKARVRFNLLEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011323.1 (pep scaffold:Pmarinus_7.0:GL477845:181126:181383:1 gene:ENSPMAG00000010296.1 transcript:ENSPMAT00000011323.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 131.724 bits (330), Expect = 2.097e-42
Identity = 54/79 (68.35%), Postives = 68/79 (86.08%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRYNIH+QLEHLQSKY+G GH+DTT++EW+ N HRD+++S +GH D L+  AI ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MSDRYNIHSQLEHLQSKYIGTGHADTTRYEWLVNQHRDSYSSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Nematostella
Match: EDO38524 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCP5])

HSP 1 Score: 124.79 bits (312), Expect = 1.730e-39
Identity = 54/79 (68.35%), Postives = 65/79 (82.28%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRYNIH+QLEHLQSKYVG GHSDTTK+EW+ N HRD+ ++ IGH + L   A+ ENETKARV+ NL+EKM+QPCG P
Sbjct:    1 MTDRYNIHSQLEHLQSKYVGTGHSDTTKFEWLVNQHRDSASAYIGHGNLLDYFALAENETKARVRFNLLEKMLQPCGTP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Nematostella
Match: EDO26019 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TD36])

HSP 1 Score: 55.4546 bits (132), Expect = 1.991e-12
Identity = 24/50 (48.00%), Postives = 32/50 (64.00%), Query Frame = 1 
Query:   94 EWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            EW+ N HRD+ ++ IGH + L   A+ ENETKARV+ NL+E      G P
Sbjct:    1 EWLVNQHRDSASAYIGHGNLLDYFALAENETKARVRFNLLEVNYVDTGLP 50
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Medaka
Match: sf3b5 (splicing factor 3b subunit 5 [Source:NCBI gene;Acc:101163684])

HSP 1 Score: 132.109 bits (331), Expect = 6.698e-42
Identity = 55/79 (69.62%), Postives = 67/79 (84.81%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPP 243
            M DRYNIH+QLEHLQSKY+G GH+DTTKWEW+ N HRD++ S +GH D L+  +I ENE+KARV+ NLMEKM+QPCGPP
Sbjct:    1 MTDRYNIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFSIAENESKARVRFNLMEKMLQPCGPP 79
[Close]
BLAST of Splicing factor 3B subunit 5 vs. Planmine SMEST
Match: SMESG000014121.1 (SMESG000014121.1)

HSP 1 Score: 171.785 bits (434), Expect = 1.030e-57
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 1 
Query:    7 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPPI 246
            MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPPI
Sbjct:    1 MGDRYNIHTQLEHLQSKYVGAGHSDTTKWEWVTNHHRDTFASIIGHNDFLSLHAIVENETKARVKLNLMEKMIQPCGPPI 80
The following BLAST results are available for this feature:
BLAST of Splicing factor 3B subunit 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SF3B53.320e-4169.62splicing factor 3b subunit 5 [Source:HGNC Symbol;A... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sf3b51.962e-4270.89gene:FBgn0040534 transcript:FBtr0082006[more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sf3b51.275e-4169.62splicing factor 3b, subunit 5 [Source:ZFIN;Acc:ZDB... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sf3b54.668e-4168.35splicing factor 3b subunit 5 [Source:NCBI gene;Acc... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sf3b57.230e-4068.35splicing factor 3b, subunit 5 [Source:MGI Symbol;A... [more]
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BLAST of Splicing factor 3B subunit 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VHI4|SF3B5_DROME1.967e-4170.89Splicing factor 3B subunit 5 OS=Drosophila melanog... [more]
sp|Q9BWJ5|SF3B5_HUMAN1.594e-4069.62Splicing factor 3B subunit 5 OS=Homo sapiens OX=96... [more]
sp|Q56K13|SF3B5_BOVIN1.594e-4069.62Splicing factor 3B subunit 5 OS=Bos taurus OX=9913... [more]
sp|Q923D4|SF3B5_MOUSE5.057e-3968.35Splicing factor 3B subunit 5 OS=Mus musculus OX=10... [more]
sp|Q55BF5|SF3B5_DICDI3.042e-3056.41Splicing factor 3B subunit 5 OS=Dictyostelium disc... [more]
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BLAST of Splicing factor 3B subunit 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KMI82.811e-4177.22Splicing factor 3B subunit 5 OS=Schistosoma manson... [more]
A0A336LKL34.176e-4177.22Splicing factor 3B subunit 5 OS=Culicoides sonoren... [more]
C1LKP94.506e-4175.95Splicing factor 3B subunit 5 OS=Schistosoma japoni... [more]
A0A430QJ556.061e-4177.22Splicing factor 3B subunit 5 OS=Schistosoma bovis ... [more]
A0A183K6M36.061e-4177.22Splicing factor 3B subunit 5 OS=Schistosoma curass... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000011323.12.097e-4268.35pep scaffold:Pmarinus_7.0:GL477845:181126:181383:1... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO385241.730e-3968.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO260191.991e-1248.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sf3b56.698e-4269.62splicing factor 3b subunit 5 [Source:NCBI gene;Acc... [more]
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BLAST of Splicing factor 3B subunit 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000014121.11.030e-57100.00SMESG000014121.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025731 ID=SMED30025731|Name=Splicing factor 3B subunit 5|organism=Schmidtea mediterranea sexual|type=transcript|length=247bp
TTTAAAATGGGTGACAGATATAATATTCATACTCAATTAGAACATTTGCA
ATCAAAATATGTTGGTGCTGGGCATTCTGATACTACTAAATGGGAATGGG
TAACAAATCATCACAGAGATACTTTTGCATCTATAATAGGACATAATGAT
TTTCTATCATTACATGCAATCGTTGAAAATGAGACAAAGGCGCGTGTAAA
ACTTAATTTAATGGAGAAAATGATTCAACCCTGTGGTCCACCTATTG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR009846SF3b5/RDS3-10
IPR017089Splicing_factor_3B_subunit_5
Vocabulary: biological process
TermDefinition
GO:0000398mRNA splicing, via spliceosome
GO:0006397mRNA processing
GO:0008380RNA splicing
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005681spliceosomal complex
GO:0005686U2 snRNP
GO:0005689U12-type spliceosomal complex
GO:0071011precatalytic spliceosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017089Splicing factor 3B, subunit 5PIRSFPIRSF037010SF3B5coord: 3..82
e-value: 7.6E-36
score: 119.8
IPR009846Splicing factor 3B subunit 5/RDS3 complex subunit 10PFAMPF07189SF3b10coord: 5..81
e-value: 6.6E-37
score: 125.5
IPR009846Splicing factor 3B subunit 5/RDS3 complex subunit 10PANTHERPTHR20978FAMILY NOT NAMEDcoord: 3..81
NoneNo IPR availablePANTHERPTHR20978:SF0SPLICING FACTOR 3B SUBUNIT 5coord: 3..81