Chaperone protein DnaJ

Overview


Name	Chaperone protein DnaJ
Smed ID	SMED30025709
Length (bp)	649

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Chaperone protein DnaJ (SMED30025709) t-SNE clustered cells

Violin plots show distribution of expression levels for Chaperone protein DnaJ (SMED30025709) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Chaperone protein DnaJ (SMED30025709) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Chaperone protein DnaJ (SMED30025709) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30025709

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30025709	h1SMcG0018396	SmedASXL_006811	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
nervous system	SMED30025709	h1SMcG0018396	dd_Smed_v4_9057_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30025709	h1SMcG0018396	dd_Smed_v4_9057_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30025709	h1SMcG0018396	dd_Smed_v4_9057_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Chaperone protein DnaJ vs. Ensembl Human
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 54.6842 bits (130), Expect = 2.488e-9
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD      E K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGE-KFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Human
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 55.0694 bits (131), Expect = 8.003e-9
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Human
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 55.0694 bits (131), Expect = 9.409e-9
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Human
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 55.8398 bits (133), Expect = 1.072e-8
Identity = 29/66 (43.94%), Postives = 41/66 (62.12%), Query Frame = 3

Query:  114 NIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
              P+     +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:   21 QTPEKPRHKMVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 84

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Human
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 55.0694 bits (131), Expect = 2.192e-8
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

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BLAST of Chaperone protein DnaJ vs. Ensembl Celegans
Match: dnj-13 (DNaJ domain (Prokaryotic heat shock protein) [Source:UniProtKB/TrEMBL;Acc:Q20774])

HSP 1 Score: 56.9954 bits (136), Expect = 1.305e-9
Identity = 27/54 (50.00%), Postives = 37/54 (68.52%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY++L +   AT +EIK AY +++ KYHPD N + G ENK K I EAY +L
Sbjct:    2 GKDYYKVLGISKGATDDEIKKAYRKMALKYHPDKNKEAGAENKFKEIAEAYDVL 55

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Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY++L +   AT +EIK AY +++ KYHPD N + G ENK K I EAY +L
Sbjct:    2 GKDYYKVLGISKGATDDEIKKAYRKMALKYHPDKNKEAGAENKFKEIAEAYDVL 55

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BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082751)

HSP 1 Score: 53.9138 bits (128), Expect = 2.457e-8
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  NATP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETGYYDILGVKPNATPDELKKAYRKLALKYHPDKNPNEGE-KFKAISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082754)

HSP 1 Score: 53.9138 bits (128), Expect = 2.457e-8
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  NATP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETGYYDILGVKPNATPDELKKAYRKLALKYHPDKNPNEGE-KFKAISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082752)

HSP 1 Score: 53.9138 bits (128), Expect = 2.457e-8
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  NATP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETGYYDILGVKPNATPDELKKAYRKLALKYHPDKNPNEGE-KFKAISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082753)

HSP 1 Score: 53.9138 bits (128), Expect = 2.457e-8
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  NATP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETGYYDILGVKPNATPDELKKAYRKLALKYHPDKNPNEGE-KFKAISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082755)

HSP 1 Score: 53.9138 bits (128), Expect = 2.457e-8
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  NATP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETGYYDILGVKPNATPDELKKAYRKLALKYHPDKNPNEGE-KFKAISQAYEVL 55

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BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
Match: dnajb1a (DnaJ heat shock protein family (Hsp40) member B1a [Source:ZFIN;Acc:ZDB-GENE-040801-90])

HSP 1 Score: 51.9878 bits (123), Expect = 1.425e-7
Identity = 25/54 (46.30%), Postives = 34/54 (62.96%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY IL +   A+ EEIK AY + + ++HPD N   G E+K K I EAY +L
Sbjct:    2 GKDYYRILGIEKGASDEEIKKAYRKQALRFHPDKNKSAGAEDKFKEIAEAYDVL 55

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BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
Match: dnajb1b (DnaJ heat shock protein family (Hsp40) member B1b [Source:ZFIN;Acc:ZDB-GENE-030131-5455])

HSP 1 Score: 51.6026 bits (122), Expect = 1.768e-7
Identity = 25/54 (46.30%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY +L +   A+ +EIK AY + + KYHPD N   G E K K I EAY +L
Sbjct:    5 GKDYYSVLGIQKGASDDEIKKAYRKQALKYHPDKNKSAGAEEKFKEIAEAYDVL 58

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BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 51.2174 bits (121), Expect = 2.829e-7
Identity = 24/56 (42.86%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   +Y++L +  +A+PEE+K AY +L+ KYHPD   T  E K K I++AY +L
Sbjct:    1 MVKETGFYDMLGVKPSASPEELKKAYRKLALKYHPDKNPTEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
Match: dnaja3a (DnaJ heat shock protein family (Hsp40) member A3a [Source:ZFIN;Acc:ZDB-GENE-030131-7837])

HSP 1 Score: 50.0618 bits (118), Expect = 5.841e-7
Identity = 23/56 (41.07%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  147 RNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL--INEAYSML 308
            R +++Y+IL +  +AT +EIK AY++++KKYHPD  +   + K K   + EAY +L
Sbjct:   88 RKQDFYQILGVPRSATQKEIKKAYYQMAKKYHPDTNKEDPQAKEKFAQLAEAYEVL 143

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Query:  147 RNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL--INEAYSML 308
            R +++Y+IL +  +AT +EIK AY++++KKYHPD  +   + K K   + EAY +L
Sbjct:   88 RKQDFYQILGVPRSATQKEIKKAYYQMAKKYHPDTNKEDPQAKEKFAQLAEAYEVL 143

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Match: chd9 (chromodomain helicase DNA binding protein 9 [Source:Xenbase;Acc:XB-GENE-1217623])

HSP 1 Score: 54.6842 bits (130), Expect = 2.381e-8
Identity = 32/87 (36.78%), Postives = 49/87 (56.32%), Query Frame = 3

Query:   51 YRLYFELQLKPLYYLMFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGN-ENKIKLINEAYSML 308
            +R+   L +   ++   RI+   P ++       K+YY+IL L S A  +EIK AY +++ KYHPD  +  N E+K K I EAY +L
Sbjct:   32 FRVGISLGILSAHFGGTRILDQKPHTLDMG----KDYYKILGLASGANEDEIKKAYRKMALKYHPDKNKDANAEDKFKEIAEAYDVL 114

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Match: dnaja4.1 (DnaJ heat shock protein family (Hsp40) member A4 [Source:NCBI gene;Acc:780309])

HSP 1 Score: 54.6842 bits (130), Expect = 2.754e-8
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+ L +  NATP+EIK AY +L+ KYHPD N + G   K KLI++AY +L
Sbjct:    1 MVKETAYYDTLGVKPNATPDEIKKAYRKLALKYHPDKNPKEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Match: dnaja4.1 (DnaJ heat shock protein family (Hsp40) member A4 [Source:NCBI gene;Acc:780309])

HSP 1 Score: 54.299 bits (129), Expect = 2.755e-8
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+ L +  NATP+EIK AY +L+ KYHPD N + G   K KLI++AY +L
Sbjct:    1 MVKETAYYDTLGVKPNATPDEIKKAYRKLALKYHPDKNPKEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Match: dnajb1 (DnaJ heat shock protein family (Hsp40) member B1 [Source:NCBI gene;Acc:493474])

HSP 1 Score: 54.299 bits (129), Expect = 3.032e-8
Identity = 27/54 (50.00%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY+IL +   AT EEIK AY + + KYHPD N   G E++ K I EAY +L
Sbjct:    2 GKDYYKILGIPKGATEEEIKKAYRKQALKYHPDKNKDPGAEDRFKEIAEAYDVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Match: prnp (prion protein [Source:Xenbase;Acc:XB-GENE-952814])

HSP 1 Score: 49.2914 bits (116), Expect = 1.632e-6
Identity = 24/50 (48.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:  159 YYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            YY+IL +  N+TP+E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    7 YYDILGVKPNSTPDELKKAYRKLALKYHPDKNPNEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Match: Dnaja4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:MGI Symbol;Acc:MGI:1927638])

HSP 1 Score: 55.0694 bits (131), Expect = 6.593e-9
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Match: Dnaja4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:MGI Symbol;Acc:MGI:1927638])

HSP 1 Score: 55.0694 bits (131), Expect = 1.436e-8
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Match: Dnaja4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:MGI Symbol;Acc:MGI:1927638])

HSP 1 Score: 55.0694 bits (131), Expect = 1.436e-8
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +V+   YY+IL +  +A+PEEIK AY +L+ KYHPD N   G   K KLI++AY +L
Sbjct:    1 MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEG--EKFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Match: Dnajb5 (DnaJ heat shock protein family (Hsp40) member B5 [Source:MGI Symbol;Acc:MGI:1930018])

HSP 1 Score: 53.1434 bits (126), Expect = 6.533e-8
Identity = 36/91 (39.56%), Postives = 50/91 (54.95%), Query Frame = 3

Query:   63 FELQLKPL--------YYLMFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            F++QL+PL         ++ FR   N   S     V  K+YY+IL + S A  +EIK AY +++ KYHPD N +   E K K I EAY +L
Sbjct:    2 FKIQLEPLKLRAWTLNGFVKFR---NKEPSTGPVAVMGKDYYKILGIPSGANEDEIKKAYRKMALKYHPDKNKEPNAEEKFKEIAEAYDVL 89

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Match: Dnajb8 (DnaJ heat shock protein family (Hsp40) member B8 [Source:MGI Symbol;Acc:MGI:1922801])

HSP 1 Score: 51.6026 bits (122), Expect = 1.007e-7
Identity = 24/54 (44.44%), Postives = 38/54 (70.37%), Query Frame = 3

Query:  156 NYYEILNLHSNATPEEIKNAYFELSKKYHPD---NLQTGNENKIKLINEAYSML 308
            NYYE+L + S+A+PE+IK AY +L+ ++HPD   + +   E K K ++EAY +L
Sbjct:    3 NYYEVLGVQSSASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKQVSEAYEVL 56

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BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Match: sp|Q9UXR9|DNAJ_METTE (Chaperone protein DnaJ OS=Methanosarcina thermophila OX=2210 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.291e-10
Identity = 29/57 (50.88%), Postives = 41/57 (71.93%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +   ++YYEIL L  +ATPE+IK +Y +L+ KYHPD N + G E K K I+EAY++L
Sbjct:    1 MATTRDYYEILGLSRDATPEDIKKSYRKLALKYHPDRNKEPGAEEKFKEISEAYAVL 57

[Close]

BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Match: sp|Q6YPM2|DNAJ_ONYPE (Chaperone protein DnaJ OS=Onion yellows phytoplasma (strain OY-M) OX=262768 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.926e-10
Identity = 30/53 (56.60%), Postives = 38/53 (71.70%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGN-ENKIKLINEAYSML 308
            K+YY IL L  +A+PE+IK AY  L+KKYHPD  +  N ENK K + EAYS+L
Sbjct:    4 KDYYHILGLDKDASPEDIKKAYRSLAKKYHPDISKEANAENKFKEVQEAYSVL 56

[Close]

BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Match: sp|A4XKA5|DNAJ_CALS8 (Chaperone protein DnaJ OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.056e-9
Identity = 32/59 (54.24%), Postives = 39/59 (66.10%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGN---ENKIKLINEAYSML 308
            + + K+YYEIL +  NAT EEIK AY  L+K+YHPD    GN   E K K INEAY +L
Sbjct:    1 MAQKKDYYEILGVPRNATQEEIKRAYRRLAKQYHPD-ANPGNKEAEEKFKEINEAYEVL 58

[Close]

BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Match: sp|B9MJZ0|DNAJ_CALBD (Chaperone protein DnaJ OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) OX=521460 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.242e-9
Identity = 32/59 (54.24%), Postives = 39/59 (66.10%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGN---ENKIKLINEAYSML 308
            + + K+YYEIL +  NAT EEIK AY  L+K+YHPD    GN   E K K INEAY +L
Sbjct:    1 MAQKKDYYEILGVPRNATEEEIKRAYRRLAKQYHPD-ANPGNKEAEEKFKEINEAYEVL 58

[Close]

BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Match: sp|Q03FR6|DNAJ_PEDPA (Chaperone protein DnaJ OS=Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w) OX=278197 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.959e-9
Identity = 29/57 (50.88%), Postives = 39/57 (68.42%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            +  NK+YY+IL +  +A+ +EIK AY +LSKKYHPD N     E K K +NEAY +L
Sbjct:    1 MAANKDYYDILGVSKDASDDEIKKAYRKLSKKYHPDINKAPDAEQKFKDVNEAYEVL 57

[Close]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Match: R7NFX5 (Chaperone protein DnaJ OS=Mycoplasma sp. CAG:776 OX=1262906 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.269e-9
Identity = 30/52 (57.69%), Postives = 39/52 (75.00%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            K+YYEIL +   AT EEIK A+ +L+K YHPDN QTG+E K K + EAY++L
Sbjct:    4 KDYYEILGVSKTATDEEIKRAFRKLAKTYHPDNKQTGDEAKFKEVGEAYAIL 55

[Close]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Match: A0A2D6L8X0 (Chaperone protein DnaJ OS=bacterium OX=1869227 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.365e-9
Identity = 28/53 (52.83%), Postives = 40/53 (75.47%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            NKNYY +L +  NA+ E+IK A+ +L++KYHPDN   G+EN+ K +NEAY +L
Sbjct:    3 NKNYYVVLGVEKNASKEDIKKAFHKLARKYHPDNKSGGDENRFKEVNEAYQIL 55

[Close]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Match: A0A420ZD24 (Chaperone protein DnaJ OS=candidate division Kazan bacterium OX=2202143 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.623e-9
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
             K+YY IL +  NA+PEEIK AY +L+ KYHPD  + G+E K K +NEAY +L
Sbjct:    2 GKDYYNILGVDRNASPEEIKKAYRKLALKYHPDKGKNGDETKFKDVNEAYQVL 54

[Close]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Match: A0A3M1FCN6 (Molecular chaperone DnaJ OS=Candidatus Woesearchaeota archaeon OX=2026803 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.249e-9
Identity = 33/54 (61.11%), Postives = 40/54 (74.07%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YYEIL +  NA+ EEIK AY  L+KKYHPD N +TG+  K K INEAYS+L
Sbjct:    2 TKDYYEILGVDKNASKEEIKKAYKRLAKKYHPDLNKETGSAEKFKEINEAYSVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Match: A0A0G4BA60 (Chaperone protein DnaJ OS=candidate division Kazan bacterium GW2011_GWA1_50_15 OX=1620412 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.822e-9
Identity = 28/53 (52.83%), Postives = 39/53 (73.58%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +K+YY+IL +   ATP+EIK AY +L+ KYHPD  + GN +K K +NEAY +L
Sbjct:    2 SKDYYQILGVAKGATPDEIKRAYRKLALKYHPDKGENGNADKFKEVNEAYQVL 54

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 51.2174 bits (121), Expect = 1.962e-8
Identity = 24/56 (42.86%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   +Y++L +  NA+P+E+K AY +L+ KYHPD   T  E K K I++AY +L
Sbjct:    1 MVKETGFYDLLGVKPNASPDELKKAYRKLALKYHPDKNPTEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Match: dnajb1a (dnaJ homolog subfamily B member 1-like [Source:NCBI gene;Acc:103024650])

HSP 1 Score: 51.9878 bits (123), Expect = 1.041e-7
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY+IL +   A+ EEIK AY + + ++HPD N   G E+K K I EAY +L
Sbjct:    3 GKDYYKILGIQKGASEEEIKKAYRKQALRFHPDKNKAPGAEDKFKEIAEAYDVL 56

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 51.6026 bits (122), Expect = 1.475e-7
Identity = 24/56 (42.86%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   +Y++L +  NA+P+E+K AY +L+ KYHPD   T  E K K I++AY +L
Sbjct:    1 MVKETGFYDLLGVKPNASPDELKKAYRKLALKYHPDKNPTEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Match: dnajb1b (dnaJ homolog subfamily B member 1 [Source:NCBI gene;Acc:103047132])

HSP 1 Score: 51.2174 bits (121), Expect = 2.060e-7
Identity = 25/54 (46.30%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY +L +   A+ +EIK AY + + KYHPD N   G E K K I EAY +L
Sbjct:    5 GKDYYSVLGIKKGASDDEIKKAYRKQALKYHPDKNKSPGAEEKFKEIAEAYDVL 58

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:NCBI gene;Acc:103041445])

HSP 1 Score: 50.447 bits (119), Expect = 3.617e-7
Identity = 26/56 (46.43%), Postives = 36/56 (64.29%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +VR   YY+IL +   A+ +EIK AY +L+ KYHPD      E K KLI++AY +L
Sbjct:    1 MVRETGYYDILGVSPKASADEIKKAYRKLALKYHPDKNPNEGE-KFKLISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 47.3654 bits (111), Expect = 9.308e-7
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY+ L ++ NAT EE+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETAYYDTLGVNPNATHEELKKAYRKLALKYHPDKNPNEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 46.595 bits (109), Expect = 1.283e-6
Identity = 23/56 (41.07%), Postives = 36/56 (64.29%), Query Frame = 3

Query:  141 IVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            +V+   YY++L +  N+T +E+K AY +L+ KYHPD      E K K I++AY +L
Sbjct:    1 MVKETAYYDLLGVKPNSTADELKKAYRKLALKYHPDKNPNEGE-KFKQISQAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
Match: dnaja3b (DnaJ heat shock protein family (Hsp40) member A3b [Source:ZFIN;Acc:ZDB-GENE-040115-3])

HSP 1 Score: 44.669 bits (104), Expect = 7.191e-6
Identity = 21/54 (38.89%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL--INEAYSML 308
            K+YY+IL +  +   ++IK +Y++L+KKYHPD  +   + K K   + EAY +L
Sbjct:   12 KDYYQILGVAKDVAQKDIKKSYYQLAKKYHPDTNKDDPKAKEKFAELAEAYEVL 65

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001704.1 (pep scaffold:Pmarinus_7.0:GL487204:33:3976:-1 gene:ENSPMAG00000001540.1 transcript:ENSPMAT00000001704.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.1282 bits (100), Expect = 7.770e-6
Identity = 23/56 (41.07%), Postives = 34/56 (60.71%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHP----DNLQTGNENKIKLINEAYSML 308
            + YYE+L +  NA+ EE+K AY  L+ ++HP    DN++   E   KLI  AY +L
Sbjct:    2 RCYYEVLGVARNASDEELKKAYRRLALQWHPDKNADNVEEATET-FKLIQAAYDVL 56

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Yeast
Match: SCJ1 (One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins [Source:SGD;Acc:S000004827])

HSP 1 Score: 50.0618 bits (118), Expect = 7.258e-8
Identity = 26/54 (48.15%), Postives = 34/54 (62.96%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLI--NEAYSML 308
            ++YY IL +  +AT +EIK+AY +LSKKYHPD      E   K I   EAY +L
Sbjct:   22 QDYYAILEIDKDATEKEIKSAYRQLSKKYHPDKNAGSEEAHQKFIEVGEAYDVL 75

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Yeast
Match: JEM1 (DnaJ-like chaperone required for nuclear membrane fusion during mating; localizes to the ER membrane; exhibits genetic interactions with KAR2 [Source:SGD;Acc:S000003609])

HSP 1 Score: 48.1358 bits (113), Expect = 3.450e-7
Identity = 24/59 (40.68%), Postives = 37/59 (62.71%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENK-------IKLINEAYSML 308
            K+YY+IL +  +A+ +EI+ AY  L+KKYHPD ++  + +K       +  INEAY  L
Sbjct:  537 KDYYKILGVSPSASSKEIRKAYLNLTKKYHPDKIKANHNDKQESIHETMSQINEAYETL 595

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Match: EDO36008 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJW2])

HSP 1 Score: 56.6102 bits (135), Expect = 1.110e-9
Identity = 31/62 (50.00%), Postives = 41/62 (66.13%), Query Frame = 3

Query:  126 SIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
            S I N+ R K+YY+IL +  NA  +EIK AYFEL+KKYHPD N       K + ++EAY +L
Sbjct:    5 SFIDNLQR-KDYYKILGVPPNANQKEIKKAYFELAKKYHPDTNKDKSASEKFQEVSEAYEVL 65

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Match: EDO46721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPE1])

HSP 1 Score: 51.6026 bits (122), Expect = 5.030e-8
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             KNYY+IL +  +A+ +E+K AY + + KYHPD N   G E K K I EAY +L
Sbjct:    2 GKNYYDILGVKKDASDQELKKAYKKQAFKYHPDKNKDPGAEEKFKEIAEAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Match: EDO31903 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWL1])

HSP 1 Score: 51.6026 bits (122), Expect = 5.203e-8
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  156 NYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSML 308
            NYYE+L +  NAT ++I+ AY  L+ KYHPD    G E   K ++EAY +L
Sbjct:    4 NYYEVLGVERNATTDDIRRAYRRLALKYHPDK-NAGTEENFKEVSEAYEVL 53

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Match: EDO49360 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG53])

HSP 1 Score: 50.0618 bits (118), Expect = 1.905e-7
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YY +LN+   A+ ++IK AY + + KYHPD N   G E K K I+EAY +L
Sbjct:    2 GKDYYAVLNVDKAASADDIKKAYRKQALKYHPDKNKSPGAEEKFKEISEAYEVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Match: EDO31958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWG4])

HSP 1 Score: 45.8246 bits (107), Expect = 4.188e-7
Identity = 25/55 (45.45%), Postives = 38/55 (69.09%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL---INEAYSML 308
            KNYY +L +   A+  +IK+AY++LS K+HPD  Q G++ K ++   I EAYS+L
Sbjct:    6 KNYYNVLGVSPKASQSKIKDAYYKLSMKHHPDRHQ-GSDKKHEVFQEIAEAYSVL 59

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Match: ENSORLT00000030433.1 (DnaJ heat shock protein family (Hsp40) member B1 [Source:NCBI gene;Acc:101155664])

HSP 1 Score: 53.5286 bits (127), Expect = 3.002e-8
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YYEIL +   A+ E+IK AY + + +YHPD N   G E+K K I EAY +L
Sbjct:    2 GKDYYEILGIKKGASDEDIKKAYRKQALRYHPDKNKSPGAEDKFKEIAEAYDVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Match: ENSORLT00000018653.2 (DnaJ heat shock protein family (Hsp40) member B1 [Source:NCBI gene;Acc:101155664])

HSP 1 Score: 53.5286 bits (127), Expect = 3.424e-8
Identity = 26/54 (48.15%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  150 NKNYYEILNLHSNATPEEIKNAYFELSKKYHPD-NLQTGNENKIKLINEAYSML 308
             K+YYEIL +   A+ E+IK AY + + +YHPD N   G E+K K I EAY +L
Sbjct:    2 GKDYYEILGIKKGASDEDIKKAYRKQALRYHPDKNKSPGAEDKFKEIAEAYDVL 55

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Match: dnaja3b (dnaJ homolog subfamily A member 3, mitochondrial [Source:NCBI gene;Acc:101170904])

HSP 1 Score: 49.6766 bits (117), Expect = 7.805e-7
Identity = 26/60 (43.33%), Postives = 40/60 (66.67%), Query Frame = 3

Query:  138 NIVRNK-NYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL--INEAYSML 308
            +I+ NK ++YE+L +  +A+ +EIK AY++L+KKYHPD      E K K   + EAY +L
Sbjct:   86 SILANKQDFYEVLGVSCSASQKEIKKAYYQLAKKYHPDTNTNDPEAKEKFAKLAEAYEVL 145

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Match: dnajc4 (DnaJ heat shock protein family (Hsp40) member C4 [Source:NCBI gene;Acc:101175412])

HSP 1 Score: 48.9062 bits (115), Expect = 8.472e-7
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:  156 NYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNE--NKIKLINEAYSML 308
            NYY++L + S+AT EEIKNA+F  SKK HPD+  +  E  ++   +NEAY +L
Sbjct:   35 NYYDLLGVKSDATLEEIKNAFFHKSKKLHPDSDPSNPELHSQFVKLNEAYRVL 87

[Close]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Match: dnajb6b (DnaJ heat shock protein family (Hsp40) member B6 [Source:NCBI gene;Acc:101159543])

HSP 1 Score: 48.9062 bits (115), Expect = 1.051e-6
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 3

Query:  159 YYEILNLHSNATPEEIKNAYFELSKKYHPD---NLQTGNENKIKLINEAYSML 308
            YY+IL +  NATPEEIK AY +L+ K+HPD   + +   E + K ++EAY +L
Sbjct:    4 YYQILGIPKNATPEEIKKAYRKLALKWHPDKNPDNKEEAERRFKELSEAYEVL 56

[Close]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Match: SMESG000028985.1 (SMESG000028985.1)

HSP 1 Score: 291.582 bits (745), Expect = 1.387e-101
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 3

Query:   96 MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLXXXXXXXXXXXXXXXXXYNHXXXXXSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK 614
            MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLSKSKKN NINNFKNPSKYNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK
Sbjct:    1 MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLSKSKKNLNINNFKNPSKYNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK 173

[Close]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Match: SMESG000028985.1 (SMESG000028985.1)

HSP 1 Score: 241.506 bits (615), Expect = 7.888e-82
Identity = 145/146 (99.32%), Postives = 145/146 (99.32%), Query Frame = 3

Query:   96 MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLXXXXXXXXXXXXXXXXXYNHXXXXXSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRL 533
            MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLSKSKKN NINNFKNPSKYNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRL
Sbjct:    1 MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKLINEAYSMLSKSKKNLNINNFKNPSKYNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRL 146

[Close]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Match: SMESG000028985.1 (SMESG000028985.1)

HSP 1 Score: 159.073 bits (401), Expect = 2.593e-50
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 3

Query:  360 YNHXXXXXSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK 614
            YNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK
Sbjct:   20 YNHYYYYYSRHESSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESIVFSSLFDKNSVSNAEEKPKNLKK 104

[Close]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Match: SMESG000017290.1 (SMESG000017290.1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.260e-9
Identity = 27/54 (50.00%), Postives = 40/54 (74.07%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGN--ENKIKLINEAYSML 308
            K+YY+IL ++ NA+P+EIK+AY++L+KKYHPD  +     E K K  +EAY +L
Sbjct:   29 KDYYKILGVNKNASPKEIKSAYYQLAKKYHPDTNKNNKDAEEKFKDASEAYEVL 82

[Close]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Match: SMESG000023241.1 (SMESG000023241.1)

HSP 1 Score: 55.0694 bits (131), Expect = 8.947e-9
Identity = 28/54 (51.85%), Postives = 36/54 (66.67%), Query Frame = 3

Query:  153 KNYYEILNLHSNATPEEIKNAYFELSKKYHPDNLQTGNENKIKL--INEAYSML 308
            KNYYEIL L  NA  +EIK  Y+EL+KKYHPD  +   E+  K   ++EAY +L
Sbjct:   36 KNYYEILGLSKNADAKEIKTKYYELAKKYHPDVNKNNKESAAKFQEVSEAYDVL 89

The following BLAST results are available for this feature:

BLAST of Chaperone protein DnaJ vs. Ensembl Human
BLAST of Chaperone protein DnaJ vs. Ensembl Celegans
BLAST of Chaperone protein DnaJ vs. Ensembl Fly
BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
BLAST of Chaperone protein DnaJ vs. TrEMBL
BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
BLAST of Chaperone protein DnaJ vs. Ensembl Yeast
BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
BLAST of Chaperone protein DnaJ vs. Planmine SMEST

BLAST of Chaperone protein DnaJ vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DNAJA4	2.488e-9	48.21	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
DNAJA4	8.003e-9	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
DNAJA4	9.409e-9	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
DNAJA4	1.072e-8	43.94	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
DNAJA4	2.192e-8	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dnj-13	1.305e-9	50.00	DNaJ domain (Prokaryotic heat shock protein) [Sou...	[more]
dnj-13	1.305e-9	50.00	DNaJ domain (Prokaryotic heat shock protein) [Sou...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Droj2	2.457e-8	46.43	gene:FBgn0038145 transcript:FBtr0082751	[more]
Droj2	2.457e-8	46.43	gene:FBgn0038145 transcript:FBtr0082754	[more]
Droj2	2.457e-8	46.43	gene:FBgn0038145 transcript:FBtr0082752	[more]
Droj2	2.457e-8	46.43	gene:FBgn0038145 transcript:FBtr0082753	[more]
Droj2	2.457e-8	46.43	gene:FBgn0038145 transcript:FBtr0082755	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dnajb1a	1.425e-7	46.30	DnaJ heat shock protein family (Hsp40) member B1a ...	[more]
dnajb1b	1.768e-7	46.30	DnaJ heat shock protein family (Hsp40) member B1b ...	[more]
dnaja1	2.829e-7	42.86	DnaJ heat shock protein family (Hsp40) member A1 [...	[more]
dnaja3a	5.841e-7	41.07	DnaJ heat shock protein family (Hsp40) member A3a ...	[more]
dnaja3a	5.841e-7	41.07	DnaJ heat shock protein family (Hsp40) member A3a ...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
chd9	2.381e-8	36.78	chromodomain helicase DNA binding protein 9 [Sourc...	[more]
dnaja4.1	2.754e-8	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
dnaja4.1	2.755e-8	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
dnajb1	3.032e-8	50.00	DnaJ heat shock protein family (Hsp40) member B1 [...	[more]
prnp	1.632e-6	48.00	prion protein [Source:Xenbase;Acc:XB-GENE-952814]	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Dnaja4	6.593e-9	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
Dnaja4	1.436e-8	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
Dnaja4	1.436e-8	49.12	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
Dnajb5	6.533e-8	39.56	DnaJ heat shock protein family (Hsp40) member B5 [...	[more]
Dnajb8	1.007e-7	44.44	DnaJ heat shock protein family (Hsp40) member B8 [...	[more]

BLAST of Chaperone protein DnaJ vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9UXR9\|DNAJ_METTE	2.291e-10	50.88	Chaperone protein DnaJ OS=Methanosarcina thermophi...	[more]
sp\|Q6YPM2\|DNAJ_ONYPE	3.926e-10	56.60	Chaperone protein DnaJ OS=Onion yellows phytoplasm...	[more]
sp\|A4XKA5\|DNAJ_CALS8	2.056e-9	54.24	Chaperone protein DnaJ OS=Caldicellulosiruptor sac...	[more]
sp\|B9MJZ0\|DNAJ_CALBD	2.242e-9	54.24	Chaperone protein DnaJ OS=Caldicellulosiruptor bes...	[more]
sp\|Q03FR6\|DNAJ_PEDPA	2.959e-9	50.88	Chaperone protein DnaJ OS=Pediococcus pentosaceus ...	[more]

BLAST of Chaperone protein DnaJ vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
R7NFX5	1.269e-9	57.69	Chaperone protein DnaJ OS=Mycoplasma sp. CAG:776 O...	[more]
A0A2D6L8X0	1.365e-9	52.83	Chaperone protein DnaJ OS=bacterium OX=1869227 GN=...	[more]
A0A420ZD24	2.623e-9	54.72	Chaperone protein DnaJ OS=candidate division Kazan...	[more]
A0A3M1FCN6	3.249e-9	61.11	Molecular chaperone DnaJ OS=Candidatus Woesearchae...	[more]
A0A0G4BA60	3.822e-9	52.83	Chaperone protein DnaJ OS=candidate division Kazan...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dnaja1	1.962e-8	42.86	DnaJ heat shock protein family (Hsp40) member A1 [...	[more]
dnajb1a	1.041e-7	46.30	dnaJ homolog subfamily B member 1-like [Source:NCB...	[more]
dnaja1	1.475e-7	42.86	DnaJ heat shock protein family (Hsp40) member A1 [...	[more]
dnajb1b	2.060e-7	46.30	dnaJ homolog subfamily B member 1 [Source:NCBI gen...	[more]
DNAJA4	3.617e-7	46.43	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4


Match Name	E-value	Identity	Description
DNAJA4	9.308e-7	44.64	DnaJ heat shock protein family (Hsp40) member A4 [...	[more]
dnaja1	1.283e-6	41.07	DnaJ heat shock protein family (Hsp40) member A1 [...	[more]
dnaja3b	7.191e-6	38.89	DnaJ heat shock protein family (Hsp40) member A3b ...	[more]
ENSPMAT00000001704.1	7.770e-6	41.07	pep scaffold:Pmarinus_7.0:GL487204:33:3976:-1 gene...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
SCJ1	7.258e-8	48.15	One of several homologs of bacterial chaperone Dna...	[more]
JEM1	3.450e-7	40.68	DnaJ-like chaperone required for nuclear membrane ...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO36008	1.110e-9	50.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46721	5.030e-8	48.15	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31903	5.203e-8	47.06	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49360	1.905e-7	46.30	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31958	4.188e-7	45.45	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Chaperone protein DnaJ vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000030433.1	3.002e-8	48.15	DnaJ heat shock protein family (Hsp40) member B1 [...	[more]
ENSORLT00000018653.2	3.424e-8	48.15	DnaJ heat shock protein family (Hsp40) member B1 [...	[more]
dnaja3b	7.805e-7	43.33	dnaJ homolog subfamily A member 3, mitochondrial [...	[more]
dnajc4	8.472e-7	49.06	DnaJ heat shock protein family (Hsp40) member C4 [...	[more]
dnajb6b	1.051e-6	47.17	DnaJ heat shock protein family (Hsp40) member B6 [...	[more]

BLAST of Chaperone protein DnaJ vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000028985.1	1.387e-101	99.42	SMESG000028985.1	[more]
SMESG000028985.1	7.888e-82	99.32	SMESG000028985.1	[more]
SMESG000028985.1	2.593e-50	100.00	SMESG000028985.1	[more]
SMESG000017290.1	1.260e-9	50.00	SMESG000017290.1	[more]
SMESG000023241.1	8.947e-9	51.85	SMESG000023241.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30025709 ID=SMED30025709|Name=Chaperone protein DnaJ|organism=Schmidtea mediterranea sexual|type=transcript|length=649bp
ATTATATATTATGTAATTATTTTAAATTATAATTATTATTTGGTTTAATA
TATAGATTGTATTTCGAATTGCAATTGAAGCCTTTATACTATTTGATGTT
TAGAATTGTAGTTAACATACCTAAATCTATTATTTTCAATATTGTTCGAA
ACAAAAATTATTATGAAATTCTTAATTTGCATAGCAATGCAACTCCCGAA
GAAATAAAAAATGCTTATTTTGAGCTCTCGAAAAAATACCATCCTGATAA
TTTGCAGACTGGAAATGAAAATAAAATAAAGTTAATTAATGAAGCTTACA
GTATGTTATCAAAGTCAAAGAAAAATTTTAATATCAACAATTTTAAAAAC
CCATCAAAATATAATCATTATTATTATTACTATTCACGTCATGAAAGCTC
AGATGTTAATAGACCGAAGTCGCAAAATACATTAAAACTGAAAAGAGCCG
TAATTTGGTTGTTTTTTTGTATATTTTTGGCTGAAATTGTTAATATATAT
ATTGTCTTTCTTGTAAGAAAAAATCAAAGACTAAAAGAGTCAATAGTTTT
TTCAAGCTTGTTTGATAAAAATAGTGTATCAAATGCAGAAGAAAAGCCAA
AAAATCTGAAGAAATAATACTTATCAATTCCCACATTGAATTTAGATTT

protein sequence of SMED30025709-orf-1

>SMED30025709-orf-1 ID=SMED30025709-orf-1|Name=SMED30025709-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
MFRIVVNIPKSIIFNIVRNKNYYEILNLHSNATPEEIKNAYFELSKKYHP
DNLQTGNENKIKLINEAYSMLSKSKKNFNINNFKNPSKYNHYYYYYSRHE
SSDVNRPKSQNTLKLKRAVIWLFFCIFLAEIVNIYIVFLVRKNQRLKESI
VFSSLFDKNSVSNAEEKPKNLKK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR036869	J_dom_sf
IPR001623	DnaJ_domain

Vocabulary: **molecular function**
Term	Definition
GO:0051082	unfolded protein binding
GO:0005524	ATP binding
GO:0008270	zinc ion binding
GO:0031072	heat shock protein binding
GO:0046872	metal ion binding

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **biological process**
Term	Definition
GO:0006260	DNA replication
GO:0006457	protein folding
GO:0009408	response to heat

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 56..76 score: 39.31 coord: 23..41 score: 46.01
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 20..76 e-value: 5.3E-19 score: 79.1
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 21..76 e-value: 8.9E-17 score: 61.0
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 21..93 score: 15.869
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 21..72 e-value: 1.69115E-16 score: 67.5705
IPR036869	Chaperone J-domain superfamily	GENE3D	G3DSA:1.10.287.110		coord: 12..106 e-value: 1.7E-19 score: 71.9
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	SSF46565	Chaperone J-domain	coord: 15..95
None	No IPR available	PANTHER	PTHR43096:SF10	DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED	coord: 19..79
None	No IPR available	PANTHER	PTHR43096	FAMILY NOT NAMED	coord: 19..79
None	No IPR available	TMHMM	TMhelix		coord: 118..140