Radial spoke head 3
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025672 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 17
Homology
BLAST of Radial spoke head 3 vs. Ensembl Human
Match: RSPH3 (radial spoke head 3 [Source:HGNC Symbol;Acc:HGNC:21054]) HSP 1 Score: 325.094 bits (832), Expect = 4.231e-107 Identity = 197/344 (57.27%), Postives = 259/344 (75.29%), Query Frame = 2 Query: 86 TYTFSSHPSAIQ-NRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD 1114 TYT++S P A+ R++YRD +E + YGN+MYDRRV+RGNT++ P+ +PD +ELQRQ+E+R+RA+A+K+A+EQLRP+TPE V+GR+H DVQTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL+G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R QR + ELRN+E+ E QRLEEQ+RR REEK RR +QQ EI+ E+++KIAA+ FAQ YL DL+P+VFG+L D+GYF+DP+ERD+E FLPWLM+ V + +E R VLD++IRE V R Y + E+ + DE GG +T+ Sbjct: 15 TYTYTSRPRALPCQRSRYRDSLTQPDEE-PMHYGNIMYDRRVIRGNTYALQTGPLLGRPDSLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEP----GGPGAMTE 353
BLAST of Radial spoke head 3 vs. Ensembl Human
Match: RSPH3 (radial spoke head 3 [Source:HGNC Symbol;Acc:HGNC:21054]) HSP 1 Score: 325.865 bits (834), Expect = 1.349e-105 Identity = 205/382 (53.66%), Postives = 270/382 (70.68%), Query Frame = 2 Query: 11 SHSK*QLTNSNFKMTQVLGPQVPD-------------QTYTFSSHPSAIQ-NRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD 1114 SH+ T K+ + G Q PD TYT++S P A+ R++YRD +E + YGN+MYDRRV+RGNT++ P+ +PD +ELQRQ+E+R+RA+A+K+A+EQLRP+TPE V+GR+H DVQTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL+G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R QR + ELRN+E+ E QRLEEQ+RR REEK RR +QQ EI+ E+++KIAA+ FAQ YL DL+P+VFG+L D+GYF+DP+ERD+E FLPWLM+ V + +E R VLD++IRE V R Y + E+ + DE GG +T+ Sbjct: 119 SHNTLAPTCFKAKLHRKRGSQPPDMASALTDRTSRAPSTYTYTSRPRALPCQRSRYRDSLTQPDEE-PMHYGNIMYDRRVIRGNTYALQTGPLLGRPDSLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEP----GGPGAMTE 495
BLAST of Radial spoke head 3 vs. Ensembl Human
Match: RSPH3 (radial spoke head 3 [Source:HGNC Symbol;Acc:HGNC:21054]) HSP 1 Score: 152.525 bits (384), Expect = 7.648e-42 Identity = 106/193 (54.92%), Postives = 136/193 (70.47%), Query Frame = 2 Query: 536 LFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD 1114 LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R QR + ELRN+E+ E QRLEEQ+RR REEK RR +QQ EI+ E+++KIAA+ FAQ YL DL+P+VFG+L D+GYF+DP+ERD+E FLPWLM+ V + +E R VLD++IRE V R Y + E+ + DE GG +T+ Sbjct: 69 LFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEP----GGPGAMTE 257 HSP 2 Score: 53.5286 bits (127), Expect = 3.722e-7 Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2 Query: 86 TYTFSSHPSAIQ-NRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPI 247 TYT++S P A+ R++YRD +E + YGN+MYDRRV+RGNT++ P+ Sbjct: 15 TYTYTSRPRALPCQRSRYRDSLTQPDEE-PMHYGNIMYDRRVIRGNTYALQTGPL 68
BLAST of Radial spoke head 3 vs. Ensembl Fly
Match: CG32392 (gene:FBgn0052392 transcript:FBtr0076947) HSP 1 Score: 157.532 bits (397), Expect = 3.581e-43 Identity = 124/344 (36.05%), Postives = 185/344 (53.78%), Query Frame = 2 Query: 26 QLTNSNFKMTQVLGPQVP-DQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQP--DPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERD-VEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEE 1045 ++T K + P P Q + ++SHP I+ + Y N+MYDRRV++G+ L P EL+R+ R+R + + R L TP V GR+H+ +QTE YLE+L+ R E ++ QTD FL++PPTP F+PAK G DV+TEI +G+LFHFD E +PI++VLV +EQS LEV E EL + R +Q FL R AE E +RLE ++ RL+ EK RR++Q L+ E + + A Q ++ LVP V + D V+++ + +S PWL V +E+ ++R +L II+E + R Y +E Sbjct: 31 EVTEMQLKWHENPCPAPPCKQAFWWASHPKPIKIKG-----CPKPVVPPPEPYKNVMYDRRVIKGSNFGNASLVTDVDPFDKAAELRRRNMLRKRTMQCRNQRNVL--GTPPPVKGRKHETIQTEKYLEKLVQRPPEFTIDTQTDLFLEKPPTPPFIPAKVGVDVATEIGEGELFHFDAEAQPIIDVLVDACIEQSMLEVAHEMELSSLRRKQEEFLAQREAELAELRRLEAEELRLQAEKERRLRQDAIAKELDAEMQKSVTAAKLLQGHIASLVPEVLENIEPAS---DAVKKEQLMKSVCPWLSAEVAEEVGHIVDSREILTAIIQEIIKQRAEIYAGYKE 364
BLAST of Radial spoke head 3 vs. Ensembl Fly
Match: CG32392 (gene:FBgn0052392 transcript:FBtr0076948) HSP 1 Score: 151.754 bits (382), Expect = 1.222e-39 Identity = 115/292 (39.38%), Postives = 168/292 (57.53%), Query Frame = 2 Query: 179 YGNLMYDRRVVRGNTHSQHLLPITAQP--DPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERD-VEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEE 1045 Y N+MYDRRV++G+ L P EL+R+ R+R + + R L TP V GR+H+ +QTE YLE+L+ R E ++ QTD FL++PPTP F+PAK G DV+TEI +G+LFHFD E +PI++VLV +EQS LEV E EL + R +Q FL R AE E +RLE ++ RL+ EK RR++Q L+ E + + A Q ++ LVP V + D V+++ + +S PWL V +E+ ++R +L II+E + R Y +E Sbjct: 326 YKNVMYDRRVIKGSNFGNASLVTDVDPFDKAAELRRRNMLRKRTMQCRNQRNVL--GTPPPVKGRKHETIQTEKYLEKLVQRPPEFTIDTQTDLFLEKPPTPPFIPAKVGVDVATEIGEGELFHFDAEAQPIIDVLVDACIEQSMLEVAHEMELSSLRRKQEEFLAQREAELAELRRLEAEELRLQAEKERRLRQDAIAKELDAEMQKSVTAAKLLQGHIASLVPEVLENIEPAS---DAVKKEQLMKSVCPWLSAEVAEEVGHIVDSREILTAIIQEIIKQRAEIYAGYKE 612
BLAST of Radial spoke head 3 vs. Ensembl Zebrafish
Match: rsph3 (radial spoke head 3 [Source:ZFIN;Acc:ZDB-GENE-040724-173]) HSP 1 Score: 317.775 bits (813), Expect = 1.311e-105 Identity = 186/317 (58.68%), Postives = 251/317 (79.18%), Query Frame = 2 Query: 89 YTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIEL--QRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYH 1033 YTFSS P A+ NR KYR + E + YGN+MYDRRVVRGNT++QH++PIT+QPDP+E Q+QQE+RRRA++++R +EQ KTP+ ++GR++ DVQTELYLEEL DRIEE VE QTD FLD+P TPLF+PAKSG+DV+T+I +G+LF FD EVKP+L+VLVGKT+EQ+ LEV+EEEEL++ R Q++ F E+R+AE VE QRLEE++RRLR EK R++QQ ++ EKE+A+KIAA+ FAQ YL+DL+P+V+ +L D GYF+DPV+RD+E FLPWLM+ V LE+ AR+VLD++I++ R A+H Sbjct: 16 YTFSSRPRAVPNRPKYRQADPSLNEEQS-SYGNIMYDRRVVRGNTYAQHIIPITSQPDPVEFFRQQQQEARRRAMSRRRLKEQFNSKTPDPLEGRKNIDVQTELYLEELSDRIEEVSVECQTDFFLDKPATPLFIPAKSGRDVATQIEEGELFDFDLEVKPMLQVLVGKTIEQALLEVMEEEELDSLRAQKKAFKEIRDAELVEVQRLEERERRLRVEKENRIKQQKAMLEKEKETADKIAARAFAQQYLSDLLPSVYSSLRDCGYFYDPVQRDIETGFLPWLMEEVGNTLEKRYVARMVLDMLIQDVTNQRSEAFH 331
BLAST of Radial spoke head 3 vs. Ensembl Zebrafish
Match: rsph3 (radial spoke head 3 [Source:ZFIN;Acc:ZDB-GENE-040724-173]) HSP 1 Score: 317.775 bits (813), Expect = 1.311e-105 Identity = 186/317 (58.68%), Postives = 251/317 (79.18%), Query Frame = 2 Query: 89 YTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIEL--QRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYH 1033 YTFSS P A+ NR KYR + E + YGN+MYDRRVVRGNT++QH++PIT+QPDP+E Q+QQE+RRRA++++R +EQ KTP+ ++GR++ DVQTELYLEEL DRIEE VE QTD FLD+P TPLF+PAKSG+DV+T+I +G+LF FD EVKP+L+VLVGKT+EQ+ LEV+EEEEL++ R Q++ F E+R+AE VE QRLEE++RRLR EK R++QQ ++ EKE+A+KIAA+ FAQ YL+DL+P+V+ +L D GYF+DPV+RD+E FLPWLM+ V LE+ AR+VLD++I++ R A+H Sbjct: 16 YTFSSRPRAVPNRPKYRQADPSLNEEQS-SYGNIMYDRRVVRGNTYAQHIIPITSQPDPVEFFRQQQQEARRRAMSRRRLKEQFNSKTPDPLEGRKNIDVQTELYLEELSDRIEEVSVECQTDFFLDKPATPLFIPAKSGRDVATQIEEGELFDFDLEVKPMLQVLVGKTIEQALLEVMEEEELDSLRAQKKAFKEIRDAELVEVQRLEERERRLRVEKENRIKQQKAMLEKEKETADKIAARAFAQQYLSDLLPSVYSSLRDCGYFYDPVQRDIETGFLPWLMEEVGNTLEKRYVARMVLDMLIQDVTNQRSEAFH 331
BLAST of Radial spoke head 3 vs. Ensembl Xenopus
Match: nr0b2 (nuclear receptor subfamily 0 group B member 2 [Source:Xenbase;Acc:XB-GENE-479354]) HSP 1 Score: 357.066 bits (915), Expect = 3.354e-120 Identity = 210/325 (64.62%), Postives = 257/325 (79.08%), Query Frame = 2 Query: 50 MTQVLGPQ--VPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDR 1018 MT V+ Q + TYTFSS P +QNR KYR V E + YGN+MYDRRVVRGNT++QH LP++AQPDPIE QRQQE++RRA+AKKRA+E LRP+TPEAV+GR+H DVQTELYLEEL DR+EE D+E QTD FLDRPPTPLF+PAK+G DV+T+IL+G+LF FD EVKP+LEVLVGKT+EQ+ LEV+EEEEL R QQR F ELRNAE E RLEEQ+RR REEK RR +Q EI+ EKE+AEK+AA+ FAQ YL DLVP+VF +L DNGYF+DPVERDVE +F+PWLM+ V +++ + R +LD+IIR+ V R Sbjct: 1 MTSVIPRQKEMGSGTYTFSSQPRVVQNRRKYRGVQPTVNEE-SFTYGNIMYDRRVVRGNTYAQHTLPLSAQPDPIEFQRQQEAQRRALAKKRAKELLRPRTPEAVEGRKHIDVQTELYLEELSDRVEEKDMECQTDAFLDRPPTPLFIPAKTGADVATQILEGELFDFDLEVKPMLEVLVGKTIEQALLEVMEEEELAQLRAQQRAFEELRNAELAEAHRLEEQERRHREEKERRKKQHREILLKEKETAEKVAARAFAQQYLADLVPSVFSSLRDNGYFYDPVERDVETTFMPWLMEEVENIMKKNDLGRTMLDMIIRDVVKKR 324
BLAST of Radial spoke head 3 vs. Ensembl Xenopus
Match: cdk2ap1 (cyclin-dependent kinase 2 associated protein 1 [Source:Xenbase;Acc:XB-GENE-5715100]) HSP 1 Score: 96.2857 bits (238), Expect = 2.129e-22 Identity = 85/278 (30.58%), Postives = 131/278 (47.12%), Query Frame = 2 Query: 170 TLRYGNLMYDRRVVRGNTHS--QHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIII 997 +L YGN+MY RR+V+G+TH+ ++ + + P+E+QRQQE E L+P+TP++ +GR L E+ D++ T F F+PAK+ +T+IL+G+LF F E T++Q+ EV+EEEEL R QQ + + I L + S YL DLV VF +L +NGYF+DPVERDV +F+PWLM V + + ++N+ +L ++ Sbjct: 14 SLTYGNIMYHRRLVQGHTHTFFTYVTFVCSTEPPVEIQRQQE------------ELLQPRTPDSEEGRHIDMCPLTLGFEK--------DMKCHTKVF-------SFIPAKT--RAATQILEGELFDFKLE-----------TIKQALREVMEEEELAQLRAQQSVL------------------------------KSCVIQNLPRHS-----------DYLVDLVSFVFCSLRENGYFYDPVERDVAPAFMPWLMKKV-ENIMKKNDLVYLLKYVL 209
BLAST of Radial spoke head 3 vs. Ensembl Xenopus
Match: cdk2ap1 (cyclin-dependent kinase 2 associated protein 1 [Source:Xenbase;Acc:XB-GENE-5715100]) HSP 1 Score: 62.3882 bits (150), Expect = 7.718e-11 Identity = 25/39 (64.10%), Postives = 30/39 (76.92%), Query Frame = 2 Query: 827 YLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAV 943 YL DLV VF +L +NGYF+DPVERDV +F+PWLM V Sbjct: 127 YLVDLVSFVFCSLRENGYFYDPVERDVAPAFMPWLMKKV 165
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Match: Rsph3b (radial spoke 3B homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:3630308]) HSP 1 Score: 290.812 bits (743), Expect = 2.580e-94 Identity = 196/375 (52.27%), Postives = 267/375 (71.20%), Query Frame = 2 Query: 89 YTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ + QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 15 YTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYALPTGQVPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 382
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Match: Rsph3a (radial spoke 3A homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1914082]) HSP 1 Score: 290.426 bits (742), Expect = 1.503e-92 Identity = 199/395 (50.38%), Postives = 274/395 (69.37%), Query Frame = 2 Query: 29 LTNSNFKMTQVLGPQVPDQTYTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 +T+ N + + G YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ + QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 128 MTDPNPRTAEASG------LYTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYALPTGQVPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 509
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Match: Rsph3b (radial spoke 3B homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:3630308]) HSP 1 Score: 290.041 bits (741), Expect = 3.416e-92 Identity = 196/375 (52.27%), Postives = 267/375 (71.20%), Query Frame = 2 Query: 89 YTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ + QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 160 YTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYALPTGQVPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 527
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Match: Rsph3a (radial spoke 3A homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1914082]) HSP 1 Score: 287.345 bits (734), Expect = 1.875e-91 Identity = 201/397 (50.63%), Postives = 275/397 (69.27%), Query Frame = 2 Query: 29 LTNSNFKMTQVLGPQVPDQTYTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPIT--AQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 +T+ N + + G YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ LP QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 128 MTDPNPRTAEASG------LYTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYA---LPTGQPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 508
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Match: Rsph3a (radial spoke 3A homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1914082]) HSP 1 Score: 149.443 bits (376), Expect = 3.387e-43 Identity = 69/93 (74.19%), Postives = 83/93 (89.25%), Query Frame = 2 Query: 326 EQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQS 604 EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS Sbjct: 2 EQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQS 94
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Match: sp|Q86UC2|RSPH3_HUMAN (Radial spoke head protein 3 homolog OS=Homo sapiens OX=9606 GN=RSPH3 PE=1 SV=1) HSP 1 Score: 325.865 bits (834), Expect = 6.479e-105 Identity = 205/382 (53.66%), Postives = 270/382 (70.68%), Query Frame = 2 Query: 11 SHSK*QLTNSNFKMTQVLGPQVPD-------------QTYTFSSHPSAIQ-NRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD 1114 SH+ T K+ + G Q PD TYT++S P A+ R++YRD +E + YGN+MYDRRV+RGNT++ P+ +PD +ELQRQ+E+R+RA+A+K+A+EQLRP+TPE V+GR+H DVQTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL+G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R QR + ELRN+E+ E QRLEEQ+RR REEK RR +QQ EI+ E+++KIAA+ FAQ YL DL+P+VFG+L D+GYF+DP+ERD+E FLPWLM+ V + +E R VLD++IRE V R Y + E+ + DE GG +T+ Sbjct: 119 SHNTLAPTCFKAKLHRKRGSQPPDMASALTDRTSRAPSTYTYTSRPRALPCQRSRYRDSLTQPDEE-PMHYGNIMYDRRVIRGNTYALQTGPLLGRPDSLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEP----GGPGAMTE 495
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Match: sp|A8E4N3|RSPH3_BOVIN (Radial spoke head protein 3 homolog OS=Bos taurus OX=9913 GN=RSPH3 PE=2 SV=1) HSP 1 Score: 313.923 bits (803), Expect = 8.891e-100 Identity = 200/339 (59.00%), Postives = 252/339 (74.34%), Query Frame = 2 Query: 86 TYTFSSHPSAIQ-NRNKYRDK-TKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGG 1096 TYT++S P A+ R +YRD + EE V YGN+MYDRRV+RGNT++ +P+ QPDP+E+QRQQ++RRRA A+K+A+EQLRP+TPE V+GR+H DVQTELYLEE+ DRI E D+E QTD FLD+PPTPLF+PAK+GKDV+TEIL+G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R Q + ELRN E E QRLEEQ+RR REEK RR QQQ +++ E+++KIAA+ FAQ YL DL+P+VF +L D GYF+DPVERD+E FLPWLM+ V + +E R VLD++IRE V R NL EQ E + LGG Sbjct: 202 TYTYTSRPRALPCQRRRYRDDLMQQPEEPV--HYGNIMYDRRVIRGNTYALQSVPLPGQPDPVEIQRQQQARRRAFARKQAQEQLRPRTPEPVEGRKHVDVQTELYLEEIADRIVEVDMECQTDAFLDKPPTPLFIPAKTGKDVATEILEGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRASQYAYEELRNIELAEVQRLEEQERRHREEKERRKQQQWQVVHKHNETSQKIAARAFAQRYLADLLPSVFDSLRDGGYFYDPVERDIEIGFLPWLMNEVDKTMESSMVGRTVLDMLIREVVERR----LNLYEQKEGRHASVRPENGLGG 534
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Match: sp|Q9DA80|RSH3B_MOUSE (Radial spoke head protein 3 homolog B OS=Mus musculus OX=10090 GN=Rsph3b PE=2 SV=1) HSP 1 Score: 290.812 bits (743), Expect = 1.805e-93 Identity = 196/375 (52.27%), Postives = 267/375 (71.20%), Query Frame = 2 Query: 89 YTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ + QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 15 YTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYALPTGQVPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 382
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Match: sp|Q3UFY4|RSH3A_MOUSE (Radial spoke head protein 3 homolog A OS=Mus musculus OX=10090 GN=Rsph3a PE=2 SV=1) HSP 1 Score: 290.426 bits (742), Expect = 1.051e-91 Identity = 199/395 (50.38%), Postives = 274/395 (69.37%), Query Frame = 2 Query: 29 LTNSNFKMTQVLGPQVPDQTYTFSSHPSAIQ-NRNKYRDKT-KLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITD--INEEIDQNEMKNDISIEPMAELENQPTED 1201 +T+ N + + G YT+SS P A+ R ++RD + VEE ++ YGN+MYDRRV+RGNT++ + QPDP+ELQRQQ++RRRA+A+KRA+EQL+P+TPE V+GR+H D+QTELYLEE+ DRI E D+E QTD FLDRPPTPLF+PAK+GKDV+T+IL G+LF FD EVKP+LEVLVGKT+EQS LEV+EEEEL N R +Q + E+RN E E QRLEEQ+RR REEK RR +QQ EI+ E+ +KI+A +FA+ YL +L+P+VF L ++G+F+DP+ERD+E FLPWLM+ V + +E R VLD++IR+ V R Y + E A + + + G T+TD + Q + + +S +P E +P E+ Sbjct: 128 MTDPNPRTAEASG------LYTYSSRPRAVACQRRRHRDSILQPVEEPMS--YGNIMYDRRVIRGNTYALPTGQVPGQPDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIRNVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFYDPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKE-----AMPPGQKTNVINGPNTVTDPSVTTLHTQKPVLDRVSSQPAPSQERKPVEE 509
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Match: sp|P12759|RSP3_CHLRE (Flagellar radial spoke protein 3 OS=Chlamydomonas reinhardtii OX=3055 GN=RSP3 PE=1 SV=1) HSP 1 Score: 242.276 bits (617), Expect = 3.248e-73 Identity = 155/306 (50.65%), Postives = 198/306 (64.71%), Query Frame = 2 Query: 83 QTYTFSSHPSAIQNRN-KYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIII 997 Q YT ++ P A+Q R KYR+ E TL N+M+DRRVVRGNT++ +LP A Q Q + A KKR L P+TPEAVDGR+H D+QT++YLEEL D + EAD QTD FLDRPPTPLFVP K+G D T+I GDLF FD EV+PILEVLVGK +EQ +EVLEEEEL R Q F ++RNAE V QR+E +RR EEK RRMQQ+ E + E+ +K+AA FA+ YL+ +V TVF L +GY +DPV R+VE +F+PWL + L + AR V+D ++ Sbjct: 9 QLYTHAAEPKAVQQRRAKYRED----ETTQTLPTANIMFDRRVVRGNTYAARILPADA------TQTQTKGPSPASTKKRTTRTLPPRTPEAVDGRRHIDIQTDVYLEELTDTVPEADTSTQTDAFLDRPPTPLFVPQKTGTDAITQIENGDLFDFDFEVEPILEVLVGKVLEQGLMEVLEEEELAAMRAHQEHFEQIRNAELVATQRMEAAERRKLEEKERRMQQERERVERERVVRQKVAASAFARGYLSGIVNTVFDRLVSSGYIYDPVMREVETAFMPWLKEQAIGYLARGVVARRVVDKLV 304
BLAST of Radial spoke head 3 vs. TrEMBL
Match: A0A1I8HW35 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020095g1 PE=4 SV=1) HSP 1 Score: 402.519 bits (1033), Expect = 3.834e-134 Identity = 228/335 (68.06%), Postives = 272/335 (81.19%), Query Frame = 2 Query: 86 TYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKAL 1090 TYTFSS P A+ NR KYRD+ +GNLMYDRRV+RGNT+SQH+LP AQPDP+E+QRQQE RRRA+A+KRAREQLRPK+PEAV+GR+H D+QTELYLEEL DRIEEADV+ QTDDFLDRPP+PLFVPAKSG+D T+IL +LF+FD EVKPILEVLVGKT+EQS LEV EEEL N R QQR F ELRNAE VEQQRLEEQDRR REEK RR+QQQ E+ E+E+A KIAA+ FAQSYL DLVP+VFGTLA++GYF+DPVERDVE FLPWL+DAV +++ AR +LD ++RE + DRH AY LE +IE+ R+A+E +A+ Sbjct: 14 TYTFSSQPRAVPNRRKYRDQFAQQNSDEVPHHGNLMYDRRVIRGNTYSQHVLPAVAQPDPVEIQRQQEMRRRAMARKRAREQLRPKSPEAVEGRKHIDIQTELYLEELADRIEEADVQCQTDDFLDRPPSPLFVPAKSGEDKETQILDNELFNFDIEVKPILEVLVGKTIEQSLLEVCGEEELANLRAQQRAFEELRNAELVEQQRLEEQDRRHREEKARRVQQQREVQEKERETANKIAARAFAQSYLADLVPSVFGTLAEHGYFYDPVERDVESGFLPWLVDAVNTDIDSSVRARALLDGLLREVIRDRHTAYRALETRIEQQRMAEEDRRAM 348
BLAST of Radial spoke head 3 vs. TrEMBL
Match: V3ZT82 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_130261 PE=4 SV=1) HSP 1 Score: 396.356 bits (1017), Expect = 9.257e-133 Identity = 227/334 (67.96%), Postives = 278/334 (83.23%), Query Frame = 2 Query: 50 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRD-KTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQ 1048 M VL P+ TYTF+S P A+Q R KYR+ + L++ V +YGN+MYDRR+VRGNT++QH LP +AQPDPIE+QRQQE++RRA+A+KRA+EQLRP++PEAV+GR+H DVQTELYLEEL DR+EEADV+ QTD FLDRPP+PLF+PAK+GKDV+T+IL+GDLF FD EVKPILEVLVGKTVEQ+ LEV+EEEEL N R QQR F ELRNAE VEQQRLEEQ+RR REEK RRM+QQ E+++ EKE+AEKIAA+ FAQSYL DLVP+VFGTLADNGYF+DPVERDVEQ F+PWLM+ V +LE+ R+VLD IIRE ++R Y E++ Sbjct: 1 MAAVLPPKGDGGTYTFASQPRAVQERKKYRETRDPLIQNDVPSQYGNIMYDRRIVRGNTYAQHTLPASAQPDPIEIQRQQENKRRAMARKRAKEQLRPRSPEAVEGRKHIDVQTELYLEELSDRVEEADVDCQTDAFLDRPPSPLFIPAKTGKDVATQILEGDLFDFDIEVKPILEVLVGKTVEQALLEVMEEEELSNLRQQQRAFEELRNAELVEQQRLEEQERRHREEKERRMKQQHEVLKKEKETAEKIAARAFAQSYLADLVPSVFGTLADNGYFYDPVERDVEQGFMPWLMEQVEIQLEKSELGRMVLDGIIREVTSNRLDLYSTAEDK 334
BLAST of Radial spoke head 3 vs. TrEMBL
Match: K1QY04 (Radial spoke head protein 3-like protein OS=Crassostrea gigas OX=29159 GN=CGI_10026456 PE=4 SV=1) HSP 1 Score: 389.037 bits (998), Expect = 2.170e-129 Identity = 224/327 (68.50%), Postives = 271/327 (82.87%), Query Frame = 2 Query: 50 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAY 1030 MT VL PQ P TYTF+S P A+Q R KYRD + + +YGN+MYDRR+VRGNT++QH LP AQPDPIE+QRQQE+RRRAIA+KRA+EQLRP++P+ V+GR+H DVQTELYLEEL DR+EEADVE QTD FLDRPP+P++VPAK+G D++T+IL+GDLF FD EVKPILEVLVGKTVEQ+ LEV+EEEEL N R QQR F ELRNAE VEQQRLEEQ+RR REEK RRM+QQ EI++ EKE+++KIAA+ FAQSYL DLVPTVFGTL+DNGYF+DPVERD+EQ F+PWL+D V +L++ RLVLD IIR+ + R AY Sbjct: 1 MTTVL-PQKPQGTYTFASQPRAVQQRKKYRDANPIDQSQGPPQYGNIMYDRRIVRGNTYAQHTLPAHAQPDPIEIQRQQENRRRAIARKRAKEQLRPRSPDPVEGRKHIDVQTELYLEELSDRVEEADVEVQTDAFLDRPPSPMYVPAKTGVDITTQILEGDLFDFDIEVKPILEVLVGKTVEQALLEVMEEEELANLRSQQRAFEELRNAELVEQQRLEEQERRHREEKERRMKQQREIVKKEKETSDKIAARAFAQSYLADLVPTVFGTLSDNGYFYDPVERDIEQGFMPWLIDQVQVQLQKSQLGRLVLDGIIRDVMNQRIDAY 326
BLAST of Radial spoke head 3 vs. TrEMBL
Match: A0A1S3JCM9 (radial spoke head protein 3 homolog B OS=Lingula unguis OX=7574 GN=LOC106172118 PE=4 SV=1) HSP 1 Score: 387.111 bits (993), Expect = 2.301e-128 Identity = 226/331 (68.28%), Postives = 273/331 (82.48%), Query Frame = 2 Query: 50 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLE 1042 MT +L PQ + TYTF+S P A+Q R KYR+ + E YGN+MYDRR+VRGNT++QH LP AQPDPIE+QRQQE+RRRAIA+KRA+EQLRP++PEAV+GR+H DVQTELYLEEL DR+EEADV+ QTD FLDRPP+P+F+PAK+G D++T+IL+G+LF FD EVKPILEVLVGKTVEQS LEV+EEEEL N R QQR F ELRNAE VEQQRLEEQ+RR REEK RRM+QQ EI+R EKE+A+KIAA+ FAQSYL DLVP+VFGTL+DNGYFFDP+ERDVEQ F+PWLM+ V + LE++N RLV+D I+RE V R + E Sbjct: 1 MTTLL-PQKTEGTYTFASQPRAVQQRRKYRETEQQNEGPAP--YGNIMYDRRIVRGNTYAQHTLPARAQPDPIEIQRQQEARRRAIARKRAKEQLRPRSPEAVEGRKHIDVQTELYLEELSDRVEEADVDCQTDAFLDRPPSPMFIPAKTGVDIATQILEGELFDFDIEVKPILEVLVGKTVEQSLLEVMEEEELANLRSQQRSFEELRNAELVEQQRLEEQERRHREEKERRMKQQREILRKEKETADKIAARAFAQSYLADLVPSVFGTLSDNGYFFDPIERDVEQGFIPWLMERVAERLEKQNLGRLVVDGILREVVKQRWEHFKKYE 328
BLAST of Radial spoke head 3 vs. TrEMBL
Match: W4Y8I6 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 384.415 bits (986), Expect = 1.700e-127 Identity = 224/333 (67.27%), Postives = 266/333 (79.88%), Query Frame = 2 Query: 50 MTQVL-GPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEE 1045 MT +L PQ TYTF+S P A+ R KYRD EAV + GN+MYDRR+VRGNT++QH LP TAQPDPIE+QRQQE RRR IA KRA++QLRP++PE V+GR+H DVQTELYLEEL DR+EEADVEAQTD FLDRPPTPLFVPAK+G D++T+I +GDLF FD EVKPILEV+VGKTVEQ+ LEV+EEEEL N R QQR F ELRNAE VE QRLEEQ+RR REEK RRM+QQ E+++ EKE+AEKIAA+ FAQSYL DL+P+VFGTL D GYF+DPVERDVE F+PW+MDA + +++ R VLD IIR+ V +R AY LEE Sbjct: 1 MTTILPAPQQKSGTYTFASQPRAVPQRKKYRDPLAQNPEAVPI--GNIMYDRRIVRGNTYAQHTLPATAQPDPIEIQRQQEIRRRKIAAKRAKDQLRPRSPEPVEGRKHIDVQTELYLEELSDRVEEADVEAQTDAFLDRPPTPLFVPAKTGMDIATQIYEGDLFDFDIEVKPILEVIVGKTVEQALLEVMEEEELANLRAQQRAFEELRNAELVETQRLEEQERRHREEKERRMKQQREVLKKEKETAEKIAARAFAQSYLADLIPSVFGTLNDKGYFYDPVERDVELGFMPWIMDAFAKCIDKSVLGRTVLDGIIRDVVKERMAAYFKLEE 331
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Match: rsph3 (radial spoke head protein 3 homolog [Source:NCBI gene;Acc:103033990]) HSP 1 Score: 313.923 bits (803), Expect = 2.309e-104 Identity = 192/326 (58.90%), Postives = 244/326 (74.85%), Query Frame = 2 Query: 50 MTQVLGP--QVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRH 1021 M L P Q P+ Y FSS P R KY+D +++ + YGN+MYDRRVVRGNT++QH LP+ +QPDP EL++QQE+RRRA+A+KRA+EQ R TPEA +GR+H+DVQTELYLEEL D E+A + QTD FLD P TPLF+PAKSGKDV+T+I +G+LF+FDTEV+P+LEVL+GKT+EQ+ LEVLEEEEL + R QQR F ELRNAE VE QRLEEQ+RR REEK RR++QQ E++ E+ AEKIAA+VFAQ YL DL+P+V+ TL D GYF+DPV+RD+E FLPWLM V L++ +LD +I + R Sbjct: 1 MASALQPYNQDPNGAYVFSSRPRPAPTRLKYKDPA-VIQNEESYVYGNIMYDRRVVRGNTYAQHTLPLRSQPDPAELKKQQEARRRALARKRAKEQFRLLTPEAPEGRKHKDVQTELYLEELSDHPEDASMGCQTDAFLDEPATPLFIPAKSGKDVATQIEEGELFYFDTEVQPVLEVLIGKTMEQALLEVLEEEELASLRAQQRAFQELRNAEIVEVQRLEEQERRHREEKVRRLKQQREVLENERVIAEKIAARVFAQQYLADLLPSVYNTLRDQGYFYDPVQRDIETGFLPWLMTEVDSALKKREIVLTMLDTLIYDVANSRQ 325
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Match: ENSAMXT00000049884.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:19188901:19201011:1 gene:ENSAMXG00000036446.1 transcript:ENSAMXT00000049884.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 243.047 bits (619), Expect = 4.930e-76 Identity = 140/289 (48.44%), Postives = 179/289 (61.94%), Query Frame = 2 Query: 71 QVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMD 937 Q P+ Y FSS P R KY+D + E + YGN+MY RRVVRGNT++QH LP+ +QPDP ELQ+Q + EQ TPEA++GR+H +VQTELYLEEL D E+A V QTD FLD+P TPLF+PAKSGKDV+T+I +G+LFHFDTEV+P+LEVL+GKT+EQ+ LEVLEEEEL + R QQ + RR ++ EK+ + L DL+P+V+ TL D GYF+DPV+RD+E FLPWLMD Sbjct: 10 QAPNGAYVFSSRPRPAPTRLKYKDPAVIQNEG-SYVYGNIMYVRRVVRGNTYAQHTLPLRSQPDPAELQKQAK------------EQFCLLTPEALEGRKHTEVQTELYLEELSDHPEDASVGCQTDAFLDKPATPLFIPAKSGKDVATQIEEGELFHFDTEVQPVLEVLIGKTMEQALLEVLEEEELASLRAQQE--------------------------QERRHRE------------EKVTLPSICSAILADLLPSVYNTLKDQGYFYDPVQRDIETGFLPWLMD 247
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Match: ENSAMXT00000055330.1 (pep primary_assembly:Astyanax_mexicanus-2.0:8:15352880:15357266:1 gene:ENSAMXG00000033650.1 transcript:ENSAMXT00000055330.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 209.149 bits (531), Expect = 7.279e-65 Identity = 129/200 (64.50%), Postives = 158/200 (79.00%), Query Frame = 2 Query: 389 ELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLD 988 +LYLEEL D E+A V QTD FLD+P TPLF+PAKSGKDV+T+I +G+LFHFDTEV+P+LEVL+GKT+EQ+ LEVLEEEEL + R QQR F ELRNAE VE QRLEEQ+RR REEK RR++QQ E++ E+ AEKIAA+ FAQ YL DL+P+V+ TL + GYF+DPV+RD+E FLPWLM V LE+ A VLD Sbjct: 12 KLYLEELSDHPEDASVGCQTDAFLDKPATPLFIPAKSGKDVATQIEEGELFHFDTEVQPVLEVLIGKTMEQALLEVLEEEELASLRAQQRAFQELRNAELVEVQRLEEQERRHREEKVRRLKQQREVLENERVIAEKIAARGFAQQYLADLLPSVYNTLREQGYFYDPVQRDIETGFLPWLMAEVDNALEKREIALTVLD 211
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Match: ENSAMXT00000047988.1 (pep primary_assembly:Astyanax_mexicanus-2.0:8:15353402:15361480:1 gene:ENSAMXG00000033650.1 transcript:ENSAMXT00000047988.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 210.305 bits (534), Expect = 1.145e-64 Identity = 130/206 (63.11%), Postives = 159/206 (77.18%), Query Frame = 2 Query: 371 HQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLD 988 H +LYLEEL D E+A V QTD FLD+P TPLF+PAKSGKDV+T+I +G+LFHFDTEV+P+LEVL+GKT+EQ+ LEVLEEEEL + R QQR F ELRNAE VE QRLEEQ+RR REEK RR++QQ E++ E+ AEKIAA+ FAQ YL DL+P+V+ TL + GYF+DPV+RD+E FLPWLM V LE+ A VLD Sbjct: 22 HSFSPKKLYLEELSDHPEDASVGCQTDAFLDKPATPLFIPAKSGKDVATQIEEGELFHFDTEVQPVLEVLIGKTMEQALLEVLEEEELASLRAQQRAFQELRNAELVEVQRLEEQERRHREEKVRRLKQQREVLENERVIAEKIAARGFAQQYLADLLPSVYNTLREQGYFYDPVQRDIETGFLPWLMAEVDNALEKREIALTVLD 227
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Match: ENSAMXT00000043046.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:29960739:29963784:-1 gene:ENSAMXG00000029064.1 transcript:ENSAMXT00000043046.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 194.512 bits (493), Expect = 1.459e-59 Identity = 118/183 (64.48%), Postives = 145/183 (79.23%), Query Frame = 2 Query: 440 AQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLD 988 QTD FLDRP TPLF+PAKSGKDV+T+I +G+LF FDTEV+P+LEVL+GKTVEQ+ L+VLEEEEL + R QQR F ELRNAE VE QRLEEQ+RR REEK RR++QQ E++ E+ AEKIAA+ FAQ YL DL+P+V+ TL D GYF+DPV+RD+E FLPWLM V LE++ A + D Sbjct: 2 CQTDAFLDRPATPLFIPAKSGKDVATQIEEGELFDFDTEVQPVLEVLIGKTVEQALLDVLEEEELASLRSQQRAFQELRNAELVEVQRLEEQERRHREEKVRRLKQQREVLENERVIAEKIAARGFAQQYLADLLPSVYNTLRDQGYFYDPVQRDIETGFLPWLMAEVDSALEKQETALTMFD 184
BLAST of Radial spoke head 3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010457.1 (pep scaffold:Pmarinus_7.0:GL482018:8229:17275:1 gene:ENSPMAG00000009466.1 transcript:ENSPMAT00000010457.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 302.753 bits (774), Expect = 1.564e-100 Identity = 194/339 (57.23%), Postives = 245/339 (72.27%), Query Frame = 2 Query: 50 MTQVLGPQVPDQTYTFSSHPSAIQNRNKY-RDKTKLVE-EAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLAD------NGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLE 1042 MT L Q + YTF+S P + R Y R+ L + + R+GN+MYDRRVVRGNT++QHL P +A PDP+ELQRQQESRRRA+A+KRA+ LRP +PE V+GR+H DVQTELYLEEL R++E D QTD FLDRP +PLF+PAKSG+D +T+IL G+LF F+ EV+P++EVLVGKTVEQS LEV+EEEEL + R QQR FLELRNAE+ E QRL EQDRRLR EK+RR +QQ E++ EKE A+K+AA+ FA+ YL DL+P VF TL D + F V DVE +F+PWLM+AV EL++ EAR +LD IIR+ R +Y E Sbjct: 1 MTAFLA-QAQSEAYTFTSRPRPVPLRAPYSRESPSLYSVDDGSDRFGNIMYDRRVVRGNTYAQHLQPTSAGPDPVELQRQQESRRRALARKRAKAALRPFSPEPVEGRKHVDVQTELYLEELSGRVQERDWGCQTDAFLDRPASPLFIPAKSGRDATTQILPGELFDFEVEVRPLVEVLVGKTVEQSLLEVMEEEELASLRAQQRAFLELRNAERAEAQRLHEQDRRLRAEKDRRRRQQQEVVAQEKEVADKVAARAFAKRYLGDLIPAVFSTLRDICTALSDDPTFPHVSLDVENAFMPWLMNAVEAELQKALEARALLDSIIRDVAARRERSYGGGE 338
BLAST of Radial spoke head 3 vs. Ensembl Nematostella
Match: EDO41065 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5C1]) HSP 1 Score: 344.739 bits (883), Expect = 5.066e-117 Identity = 204/302 (67.55%), Postives = 250/302 (82.78%), Query Frame = 2 Query: 83 QTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLD 988 +TYTF+S P +Q R K+RD EA ++ YGN+MYDRR+VRGNT++Q LP TAQPDPIE+Q+QQE+RRRAIA+KRA++QL+P++PE V+GR+H DVQTELYLEEL DR+EEA+VE QTD FLDRPP+PLF+PAK+G DV+T+IL+G+LF FD EVKPILEVLVGKTVEQ+ LEV+EEEEL N R QQR F ELRNAE VE QRLEEQ+RR REEK RRM+QQ ++++ EKE+ EKIAA+ FAQSYL DLVP+VFG+L +NGYF+DPVERDVE F+PWLM V L + AR VLD Sbjct: 13 RTYTFASQPRPVQQRKKFRDPLTQQNEA-SMPYGNIMYDRRIVRGNTYAQRTLPATAQPDPIEIQKQQEARRRAIARKRAKQQLQPRSPEPVEGRKHIDVQTELYLEELSDRVEEAEVETQTDAFLDRPPSPLFIPAKTGVDVATQILEGELFDFDIEVKPILEVLVGKTVEQALLEVMEEEELSNLRAQQRRFEELRNAELVETQRLEEQERRHREEKERRMRQQAQVLKKEKETGEKIAARAFAQSYLADLVPSVFGSLNENGYFYDPVERDVETYFMPWLMGRVDVNLNRSLVARTVLD 313
BLAST of Radial spoke head 3 vs. Ensembl Medaka
Match: rsph3 (radial spoke head protein 3 homolog [Source:NCBI gene;Acc:101175526]) HSP 1 Score: 161.77 bits (408), Expect = 1.572e-45 Identity = 124/302 (41.06%), Postives = 157/302 (51.99%), Query Frame = 2 Query: 83 QTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLD 988 + Y FSS P +++ K R E + ++GN+M D RV RG SQ P+ P+ + R KT + E YLEEL D E + E QTD FLDRP TPLF+ AK+GKD +T+I +GDLF F+ EV+PILE+LVGKT+EQS LEV+EEEEL QQR FLE RN E + + QQ + EKE AEKIAA+ Q L DLVP+V +L GYF+DPVER F P LMD V L++ R +LD Sbjct: 14 RMYVFSSRPRVVEDPWKPRWSPS---ERMQQKHGNIMRDPRVFRGIASSQ---PLKFAVSPVLF-----------LYLSHNQSRRSKTGQEEMFSPPFGSHAESYLEELSDIPEVKETECQTDAFLDRPETPLFLAAKTGKDAATQIYEGDLFDFEEEVQPILEILVGKTLEQSLLEVMEEEELAALMAQQRAFLERRNNE----LPEVHRLEEQERRRREERVQQRRALEEEKEVAEKIAARALTQQQLADLVPSVLASLRREGYFYDPVERGEATHFFPRLMDDVESILQKNCRVRELLD 294
BLAST of Radial spoke head 3 vs. Planmine SMEST
Match: SMESG000067698.1 (SMESG000067698.1) HSP 1 Score: 737.643 bits (1903), Expect = 0.000e+0 Identity = 401/402 (99.75%), Postives = 402/402 (100.00%), Query Frame = 2 Query: 50 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNVTITDINEEIDQNEMKNDISIEPMAELENQPTEDKELKEDVSIIPNELSQIT 1255 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESRRRAIAKKRAREQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCLEVLEEEELENFRCQQRMFLELRNAEKVEQQRLEEQDRRLREEKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGN+TITDINEEIDQNEMKNDISIEPMAELENQPTEDKELKEDVSIIPNELSQIT Sbjct: 1 MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDRRVVRGNTHSQHLLPITAQPDPIELQRQQESRRRAIAKKRAREQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCLEVLEEEELENFRCQQRMFLELRNAEKVEQQRLEEQDRRLREEKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRHVAYHNLEEQIERARLADEASKALGGNITITDINEEIDQNEMKNDISIEPMAELENQPTEDKELKEDVSIIPNELSQIT 402
BLAST of Radial spoke head 3 vs. Planmine SMEST
Match: SMESG000021328.1 (SMESG000021328.1) HSP 1 Score: 169.474 bits (428), Expect = 8.261e-49 Identity = 131/320 (40.94%), Postives = 180/320 (56.25%), Query Frame = 2 Query: 86 TYTFSSHPSAIQNRNK-YRDK----TKLVEEAVTLRYGNLMYDR---RVVRGNTHSQHLLPITAQPDPIELQRQQESXXXXXXXXXXXEQLRPKTPEAVDGRQHQDVQTELYLEELIDRIEEADVEAQTDDFLDRPPTPLFVPAKSGKDVSTEILKGDLFHFDTEVKPILEVLVGKTVEQSCXXXXXXXXXXNFRCQQRMFLELRNAEKVXXXXXXXXXXXXXXXKNRRMQQQVEIIRLEKESAEKIAAKVFAQSYLTDLVPTVFGTLADNGYFFDPVERDVEQSFLPWLMDAVCQELEQENEARLVLDIIIREAVTDRH 1021 T+ F S P+ ++ K YRDK K V E T++ N Y R ++ R LP P P V GR H++VQT+LYLEEL+D+IE D QTD+FL+RP TPLF+PAKSG D+ST++ +GD+F FD EVKPI+EVLV KT+EQ+ LEV+EEEEL N RC+ ELR A E R E Q++R REE RR+++ E+ L+ E+ +KI +F+Q L L+P F L NGYF DPVE D++ F+ L+ + A +++D +IR+A TD+H Sbjct: 13 TFVFESFPTVCSDKWKPYRDKEFIEDKRVNELTTVQCKNTEYLRPLKKIPRTKGFKGGYLP-------------------------------PDLP-PVAGRLHEEVQTDLYLEELVDKIELIDKCTQTDNFLNRPSTPLFIPAKSGFDISTQVYEGDVFFFDIEVKPIIEVLVTKTIEQALLEVMEEEELANIRCEINHQGELRAAYLNEIMRREIQNQRYREEYQRRLKEAKELAALKLETGKKIITSLFSQKLLEPLMPLSFENLYVNGYFRDPVELDLQTFFMKELLRETINKTNANLRAFVMVDGLIRDA-TDQH 299 The following BLAST results are available for this feature:
BLAST of Radial spoke head 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of Radial spoke head 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Radial spoke head 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of Radial spoke head 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Radial spoke head 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of Radial spoke head 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Radial spoke head 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Radial spoke head 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Radial spoke head 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Radial spoke head 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Radial spoke head 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Radial spoke head 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Radial spoke head 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Radial spoke head 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025672 ID=SMED30025672|Name=Radial spoke head 3|organism=Schmidtea mediterranea sexual|type=transcript|length=1349bpback to top protein sequence of SMED30025672-orf-1 >SMED30025672-orf-1 ID=SMED30025672-orf-1|Name=SMED30025672-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=413bp MTQVLGPQVPDQTYTFSSHPSAIQNRNKYRDKTKLVEEAVTLRYGNLMYDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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