TSNAXIP1_N domain-containing protein

Overview
NameTSNAXIP1_N domain-containing protein
Smed IDSMED30025664
Length (bp)1582
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TSNAXIP1_N domain-containing protein (SMED30025664) t-SNE clustered cells

Violin plots show distribution of expression levels for TSNAXIP1_N domain-containing protein (SMED30025664) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TSNAXIP1_N domain-containing protein (SMED30025664) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TSNAXIP1_N domain-containing protein (SMED30025664) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025664

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30025664SMESG000060720.1 SmedASXL_008761SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30025664SMESG000060720.1 dd_Smed_v4_13434_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025664SMESG000060720.1 dd_Smed_v4_13434_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025664SMESG000060720.1 dd_Smed_v4_13434_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30025664SMESG000060720.1 dd_Smed_v6_13434_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30025664SMESG000060720.1 dd_Smed_v4_13434_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30025664SMESG000060720.1 dd_Smed_v4_13434_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Xenopus
Match: c6orf118 (chromosome 6 open reading frame 118 [Source:NCBI gene;Acc:779868])

HSP 1 Score: 59.6918 bits (143), Expect = 6.814e-9
Identity = 50/221 (22.62%), Postives = 98/221 (44.34%), Query Frame = 3 
Query:  813 PNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEM-PVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTE-------------SEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLL 1433
            P+  RL +++EIF+D+   S +FG+I   IK AY+  +E +L++   +Q E+L +E+  +++     + +E+    + ++E+   IA++ +E+                 R N   E  R  IQ  +   +HT+             S+  E  +     I   I  +++E K+ M        L   ++DI  E  K+      L+S    +  +++ LL
Sbjct:  227 PHLARLNVFSEIFEDICKNSSIFGNILRQIKIAYELNIENLLDVQSATQSEILMAEISGMKKRAVQTQDIEDALQTVRRLEQEAHIALERNEQL----------------RNNLKAELTRSSIQDTAKEILHTQVKESNTKPNVPSISDTFESKRCEVLAISAEIQGLEQEMKKNMTHAVNTELLQRHIKDIQAEAVKIKSSNEFLQSASKGLGSEMKRLL 431
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Mouse
Match: 1700010I14Rik (RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:MGI:1914181])

HSP 1 Score: 52.7582 bits (125), Expect = 8.264e-7
Identity = 59/241 (24.48%), Postives = 108/241 (44.81%), Query Frame = 3 
Query:  819 FHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLE-MPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRK--NKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREE----------DYKLIDSVMDGSANF 1502
            F+RLQ++ EIF+D+ + S +FG +   IK  Y+  +  +L+  P +Q + L +E+  L  S      ++  +  + +V++A   A++ +++  N L  E   LQ   ++    KM +EK             T  EK+E  +   +E +D I  ++K  K+ M            ++ I  E  KL      L+ ++  +  Q++ LL    I EE          +Y  I+ V D   +F
Sbjct:  276 FNRLQVFGEIFEDICNSSLIFGDLLKEIKDEYELYMAALLDSQPTAQYQRLLAEVRGLENSPRPSCDIDQAKEHLRKVQQAYLEALEHNDKLRNELEAESLLLQSAKEKAANGKMKDEKE-----------LTLVEKVEKRRCEIFEKLDEIKALEKHIKENMVHSGVFKIAESGLKSIEDEAIKLETSNRILKKKIKVIESQMKQLLLRSKISEEERQALWDLIREYSDIEDVEDVEGDF 505
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Mouse
Match: 1700010I14Rik (RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:MGI:1914181])

HSP 1 Score: 52.373 bits (124), Expect = 9.804e-7
Identity = 54/217 (24.88%), Postives = 101/217 (46.54%), Query Frame = 3 
Query:  819 FHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLE-MPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRK--NKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREED 1460
            F+RLQ++ EIF+D+ + S +FG +   IK  Y+  +  +L+  P +Q + L +E+  L  S      ++  +  + +V++A   A++ +++  N L  E   LQ   ++    KM +EK             T  EK+E  +   +E +D I  ++K  K+ M            ++ I  E  KL      L+ ++  +  Q++ LL    I EE+
Sbjct:  257 FNRLQVFGEIFEDICNSSLIFGDLLKEIKDEYELYMAALLDSQPTAQYQRLLAEVRGLENSPRPSCDIDQAKEHLRKVQQAYLEALEHNDKLRNELEAESLLLQSAKEKAANGKMKDEKE-----------LTLVEKVEKRRCEIFEKLDEIKALEKHIKENMVHSGVFKIAESGLKSIEDEAIKLETSNRILKKKIKVIESQMKQLLLRSKISEEE 462
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Mouse
Match: 1700010I14Rik (RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:MGI:1914181])

HSP 1 Score: 52.373 bits (124), Expect = 1.038e-6
Identity = 59/241 (24.48%), Postives = 108/241 (44.81%), Query Frame = 3 
Query:  819 FHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLE-MPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRK--NKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREE----------DYKLIDSVMDGSANF 1502
            F+RLQ++ EIF+D+ + S +FG +   IK  Y+  +  +L+  P +Q + L +E+  L  S      ++  +  + +V++A   A++ +++  N L  E   LQ   ++    KM +EK             T  EK+E  +   +E +D I  ++K  K+ M            ++ I  E  KL      L+ ++  +  Q++ LL    I EE          +Y  I+ V D   +F
Sbjct:  257 FNRLQVFGEIFEDICNSSLIFGDLLKEIKDEYELYMAALLDSQPTAQYQRLLAEVRGLENSPRPSCDIDQAKEHLRKVQQAYLEALEHNDKLRNELEAESLLLQSAKEKAANGKMKDEKE-----------LTLVEKVEKRRCEIFEKLDEIKALEKHIKENMVHSGVFKIAESGLKSIEDEAIKLETSNRILKKKIKVIESQMKQLLLRSKISEEERQALWDLIREYSDIEDVEDVEGDF 486
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Mouse
Match: 1700010I14Rik (RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:MGI:1914181])

HSP 1 Score: 51.6026 bits (122), Expect = 1.568e-6
Identity = 59/243 (24.28%), Postives = 109/243 (44.86%), Query Frame = 3 
Query:  819 FHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLE-MPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRK--NKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREE----------DYKLIDSVMDGSANFPL 1508
            F+RLQ++ EIF+D+ + S +FG +   IK  Y+  +  +L+  P +Q + L +E+  L  S      ++  +  + +V++A   A++ +++  N L  E   LQ   ++    KM +EK             T  EK+E  +   +E +D I  ++K  K+ M            ++ I  E  KL      L+ ++  +  Q++ LL    I EE          +Y  I+ V D   +F +
Sbjct:  163 FNRLQVFGEIFEDICNSSLIFGDLLKEIKDEYELYMAALLDSQPTAQYQRLLAEVRGLENSPRPSCDIDQAKEHLRKVQQAYLEALEHNDKLRNELEAESLLLQSAKEKAANGKMKDEKE-----------LTLVEKVEKRRCEIFEKLDEIKALEKHIKENMVHSGVFKIAESGLKSIEDEAIKLETSNRILKKKIKVIESQMKQLLLRSKISEEERQALWDLIREYSDIEDVEDVEGDFEM 394
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Match: A0A210PRQ2 (TSNAXIP1_N domain-containing protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT07161 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.734e-19
Identity = 132/521 (25.34%), Postives = 247/521 (47.41%), Query Frame = 3 
Query:  141 LKNILNKNIEDHKAEIKFIAQSSLNG---FKSQNKLENGVKWKTSIVKTKTKLKSEKSIPFHKSQAQEKD-DFTNRLFLFSLGPDEVVDKPETNKQIKPPILLKSLNKVS--RVKEMKL----DNKKPISPTYKETVCNMAVSAETFNFSSPRF---------PVRDNLRN--DEINNEEIKE--------LLP-HYIHPSFKTRYVELLRFREENIREKDEMEKEVLAGTNXXXXXXXXXXXXXXXXXXXRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQ-KELLESEMDQL-RQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEI----LQRKNK--MDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKL-------------IDSVMDGSANFPLQDHVESNKWNWYIS 1550
            L+ +LN      K +IK  A   LN    ++   +  N    KT+     TK KS+ +I     Q  E+D    + L+ FS+G    +    T++ I+PP   +S  KVS  R    K      N+   +   K+ V    +  +    SSPR          PV DN     +++     +E         LP H++  + K +Y++L+ F  + +++++  EK+VL+G   VKHLE+RL+++L ++      PNFH+LQIY+  F+DLI +S  F  I   +K+ YD+ + ++L+    Q  +LL+ ++ Q+  +     + L++ +  +  +E+  K  ++ +E+   ++ +EE  L  +    LQRK      EE   ++           ++++E  K+   E +D+++ ++ + +++    +  THL + +++  +EV+KL       E  + +M   L+  ++  D  E D K              I ++M+G A+    D  + +KWNWYIS
Sbjct:   12 LRQLLNNTEVVQKKDIKDYAGGHLNQSKLYRPPTQWANKNWNKTNPEPLSTKSKSKSTIVVSTRQPTERDHKMKDVLYDFSIGTSGSL---PTHQTIRPP---RSPRKVSESRTSTRKCPSASSNRSLYTEMEKDGVYVEELKTDEVMLSSPRITNSKGKKLPPVEDNEYTVMEDLAKSLAEEGLLTFRHTFLPTHHMGVTKKDQYIKLMSFETDILKKQESNEKKVLSGEKAVKHLEERLQQELDSMNFFDMGPNFHKLQIYSNTFEDLIEDSPTFVYILRAVKTEYDNYISKLLDTQTPQHSKLLKEQVQQMASRGTSRPDDLQDAKDRVSHLEQKAKDKLEENEKLRRMVEEEEEWLATVPEPDLQRKRSSVFREEGPAEL-----------ADEIEHAKALILEKLDDLNNLRTKLREQYVPLTVCTHLEQCIKETEVEVQKLLKQNEYFERSIEEMEVDLKEAIQQADTSERDAKRIWRKVNSRRGLPHIPNLMNGGAHESDDDEDDESKWNWYIS 515
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Match: V4AD75 (TSNAXIP1_N domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_232257 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.171e-15
Identity = 80/308 (25.97%), Postives = 152/308 (49.35%), Query Frame = 3 
Query:  663 RYVELLRFREENIREKDEMEKEVLAGTNXXXXXXXXXXXXXXXXXXXRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSI--------FNKEKLENLQSE---IVQVEEAIKIAIKTDEEFDNLLIDEEAK-LQEILQRKNKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLIDSVMDGSANFPLQDHVESNKWNWYIS 1550
            +Y    +F    IR+++  E +VL+G   V H E  L++ L  ++     PNFH+LQ+++ +FDD+  ES  F  I   IKSAYD  + ++L+   ++ +LL  +++Q+  +         F K K+E+ + E   +++  + ++ A++  EE D L +  E + + +++  +N   +E   ++           SE+L+  K+   E MD ++ +    ++     +  THL + +++  IEV+KL       E  +  M   L+  +   D  E+D + I   ++     P  D  +  KWNWYIS
Sbjct:  196 QYRTYRQFESSIIRKREAQETKVLSGNQAVFHHENHLRQGLSTLKGHVSGPNFHKLQVHSNVFDDITEESPTFKFILQDIKSAYDDYISKLLDTQTAKHQLLHDQVEQMAANAKTQPEELEFVKNKVEDYEIEAKWLLEENQRLREAVR--EETDLLALTPEIEPIPKVISVRN---DEPPAEL-----------SEELQHTKALILEKMDALNKLHANLREDHVPLTVCTHLEQCIKETEIEVQKLLKQNEYFEKSINDMEHDLKEAITDADTSEKDARRIWRKVNSLRAQPGDDDEDEGKWNWYIS 487
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Match: R7VLG1 (TSNAXIP1_N domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_221744 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.960e-15
Identity = 95/337 (28.19%), Postives = 163/337 (48.37%), Query Frame = 3 
Query:  627 LLPHYI-HPSFKTRYVELLRFREENIREKDEMEKEVLAGTNXXXXXXXXXXXXXXXXXXXRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVST-VHTE----------SEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREED----YKLIDSV---------MDGSAN-FPLQD---HVESNKWNWYIS 1550
             LP+Y+ + + + +Y ++  F  + +R++D  E++VL+G   V+HLE +LK+ L N+    + PNFHRLQ+Y+  FD LI++S  FG I   IK  YD  V            LL+S+ D+ R  ++N  ++E L S      EA+   IK     + L  +   KL E  + +  +  EK  +     V T V T           +E++E L S   E M     ++   ++     +   HL + +++  +E++KL   +   E  + +M++ L   +E  D  E D    +K + +V          DGS N + L+D     +++KW+WYIS
Sbjct:  187 FLPNYLENVTKRDQYQKMKNFESKILRKQDATEQKVLSGLKAVQHLESKLKQDLDNLPYDGYGPNFHRLQLYSNTFDALIADSITFGYILKTIKKEYDLYVS----------SLLDSQRDKHRL-LYN--QVEELYSNGASNSEALDREIK---RVNALQAEARDKLAENDRLRKALANEKYLETLPIEVPTPVRTTNYPENSELEIAEQIEQLHSQIMEKMAASKELKDRLRKDYVPSTVTQHLEQCIKETEVEIQKLTSSKDYFERSINEMQKDLHEAIEGADTTERDAKRVWKRVSAVKLLGPNLNSTDGSVNLYSLEDSDSEDDASKWSWYIS 507
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Match: A0A1S3JUG3 (uncharacterized protein LOC106176247 OS=Lingula unguis OX=7574 GN=LOC106176247 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.503e-12
Identity = 91/357 (25.49%), Postives = 159/357 (44.54%), Query Frame = 3 
Query:  609 NEEIKELLPHYIHPSFKTRYV--------------ELLRFREENIREKDEMEKEVLAGTNXXXXXXXXXXXXXXXXXXXRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEK-LENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREED----------YKLIDSVMDG-------SANFPLQ----------DHVESN-KWNWYIS 1550
            +E  + L+P +   +FK R++               L  F +  IR++D ME+EV++G   V+ LE++LK++L  +    + PNFHRLQIY+   DD+I ES  F  +   IK  YD+ +  +L+   SQ   L  ++ QL     +K K LE  +S +  +E   K  +  +E      + +E + +  L       E  +  I +         +E+++ L ++  E ++ I+  +    +K    S   HL + V++  IE++KL       + E+ +M      +++  ++ E D           K +DS   G       S   P +          D  E+  KWNWYIS
Sbjct:  226 DESPRNLIPKHPPHTFKHRFISSHLGGVTKRDQYDRLKDFEKTVIRKQDAMEREVMSGQKAVEQLEQKLKEELATLPYDGYGPNFHRLQIYSNTLDDMIRESPTFAYVLRQIKGEYDNYLSTLLDSQTSQHNKLYEQIQQLSDRGTSKPKHLEADKSSVEFMESEAKKMLSLNES-----LRKEVQHELELAAAAPEPEPPKRIIPTFVGVQKQDLAEQVQDLHTSITEKLETINATRHHLHEKFVPSSVCNHLEQCVKETEIEIQKLMKQNEFCDKEIEEMENDFHEIIDDAELSERDIRRIWRKVNAVKAVDSAKSGNTASGWTSGPTPTRAQPGQEDSDDDEKETQRKWNWYIS 577
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Match: A0A0B7AHX4 (TSNAXIP1_N domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF121720 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.246e-11
Identity = 64/267 (23.97%), Postives = 128/267 (47.94%), Query Frame = 3 
Query:  816 NFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQL--------RQSIFNKEKLENLQSE---IVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTE-SEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLIDSVMDGSANFPL----------QDHVESNKWNWYIS 1550
            + HRLQ  + I +D+  ES   G I   IK+ YD+ + ++L+   +Q+  L S+++Q+         + I   EKL+ L+ E   ++ + + + I +K D+E     ++E  ++Q +          K+       V   H E S +LE +K+   E +D ++ ++ +T+      +  +HL + +++  +EV+KL       E  + +M   L+  +   D  E+D + I   ++ +   P           +DH +++KW+WYIS
Sbjct:  242 SLHRLQTCSNILNDITQESPSLGYILHCIKTEYDTYISKLLDGQPTQEHYLHSQVNQMISLGTSRPNEMIAATEKLKALEKEAKYLLDMNQKLHIDVKLDQE----QLNETPEIQAV----------KKDVTVGGVVKEQHIELSVELENMKALILEKLDELNSLRMQTRADFVPITVCSHLEQCIKETEVEVQKLLKQNEYFERSIDEMESDLKEAIIEADTSEKDARRIWRKVNSTRGMPGVRQATGAESDEDHDDASKWDWYIS 494
[Close]
BLAST of TSNAXIP1_N domain-containing protein vs. Planmine SMEST
Match: SMESG000060720.1 (SMESG000060720.1)

HSP 1 Score: 919.457 bits (2375), Expect = 0.000e+0
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 3 
Query:  123 MTQEIPLKNILNKNIEDHKAEIKFIAQSSLNGFKSQNKLENGVKWKTSIVKTKTKLKSEKSIPFHKSQAQEKDDFTNRLFLFSLGPDEVVDKPETNKQIKPPILLKSLNKVSRVKEMKLDNKKPISPTYKETVCNMAVSAETFNFSSPRFPVRDNLRNDEINNEEIKELLPHYIHPSFKTRYVELLRFREENIREKDEMEKEVLAGTNXXXXXXXXXXXXXXXXXXXRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLIDSVMDGSANFPLQDHVESNKWNWYIS 1550
            MTQEIPLKNILNKNIEDHKAEIKFIAQSSLNGFKSQNKLENGVKWKTSIVKTKTKLKSEKSIPFHKSQAQEKDDFTN LFLFSLGPDEVVDKPETNKQIKPPILLKSLNKVSRVKEMKLDNKKPISPTYKETVCNMAVSAETFNFSSPRFPVRDNLRNDEINNEEIKELLPHYIHPSFKTRYVELLRFREENIREKDEMEKEVLAGTNLVKHLEKRLKKKLLNIEKLRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLIDSVMDGSANFPLQDHVESNKWNWYIS
Sbjct:    1 MTQEIPLKNILNKNIEDHKAEIKFIAQSSLNGFKSQNKLENGVKWKTSIVKTKTKLKSEKSIPFHKSQAQEKDDFTNHLFLFSLGPDEVVDKPETNKQIKPPILLKSLNKVSRVKEMKLDNKKPISPTYKETVCNMAVSAETFNFSSPRFPVRDNLRNDEINNEEIKELLPHYIHPSFKTRYVELLRFREENIREKDEMEKEVLAGTNLVKHLEKRLKKKLLNIEKLRHTPNFHRLQIYTEIFDDLISESWMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEKLENLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRHQIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTHLTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLIDSVMDGSANFPLQDHVESNKWNWYIS 476
The following BLAST results are available for this feature:
BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
c6orf1186.814e-922.62chromosome 6 open reading frame 118 [Source:NCBI g... [more]
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
1700010I14Rik8.264e-724.48RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:... [more]
1700010I14Rik9.804e-724.88RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:... [more]
1700010I14Rik1.038e-624.48RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:... [more]
1700010I14Rik1.568e-624.28RIKEN cDNA 1700010I14 gene [Source:MGI Symbol;Acc:... [more]
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BLAST of TSNAXIP1_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210PRQ22.734e-1925.34TSNAXIP1_N domain-containing protein OS=Mizuhopect... [more]
V4AD751.171e-1525.97TSNAXIP1_N domain-containing protein OS=Lottia gig... [more]
R7VLG15.960e-1528.19TSNAXIP1_N domain-containing protein OS=Capitella ... [more]
A0A1S3JUG31.503e-1225.49uncharacterized protein LOC106176247 OS=Lingula un... [more]
A0A0B7AHX44.246e-1123.97TSNAXIP1_N domain-containing protein (Fragment) OS... [more]
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TSNAXIP1_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000060720.10.000e+099.79SMESG000060720.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025664 ID=SMED30025664|Name=TSNAXIP1_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1582bp
CCATTCTCTAATTTATTTAACGAGAATTTTAATAAACACTTTTTAAATCT
AAACTATTTTTACTATCATCATAAAATCTATAATTGTCTGTATCTTTTAT
TCATTTTAATTTACTTTCTGAGATGACACAAGAAATCCCGTTAAAAAATA
TTTTAAATAAAAATATTGAAGATCACAAAGCAGAAATTAAATTTATTGCT
CAAAGTTCTTTAAATGGATTTAAATCTCAAAATAAATTAGAGAATGGAGT
TAAATGGAAAACATCAATTGTCAAGACAAAAACAAAATTGAAATCAGAAA
AGTCAATTCCATTTCATAAATCACAAGCTCAAGAGAAAGATGATTTTACA
AATCGTTTATTTTTATTTTCTCTTGGACCAGATGAAGTTGTTGATAAGCC
AGAAACAAATAAACAAATAAAGCCTCCAATTTTGCTGAAATCACTCAATA
AAGTAAGCCGAGTAAAAGAAATGAAATTGGATAATAAAAAACCAATCAGC
CCAACCTACAAAGAAACGGTTTGTAATATGGCTGTATCGGCTGAAACATT
CAATTTCTCGTCACCTCGCTTTCCGGTTCGAGACAATCTGAGAAATGACG
AGATTAATAATGAAGAAATTAAAGAACTTTTGCCACATTATATTCATCCG
AGCTTTAAGACTCGCTACGTTGAGTTGCTGAGGTTTAGGGAGGAAAACAT
TAGGGAAAAAGATGAAATGGAGAAAGAAGTTTTGGCAGGCACAAATCTTG
TAAAACATCTAGAAAAACGACTGAAAAAAAAACTACTAAACATTGAAAAG
CTTCGGCATACTCCAAATTTCCACCGTCTTCAGATCTACACAGAAATTTT
TGATGATCTTATTTCAGAGTCATGGATGTTTGGTTCCATATTCTCTATGA
TAAAGAGTGCATACGATTCCGAAGTAGAAAGAGTTTTAGAAATGCCTGTT
TCCCAGAAAGAACTACTTGAATCTGAAATGGATCAGTTGAGACAGTCAAT
ATTTAACAAAGAAAAACTGGAGAACTTACAGTCAGAAATAGTTCAAGTTG
AAGAAGCTATTAAGATTGCCATTAAAACAGATGAAGAATTTGACAACTTA
TTAATTGATGAAGAAGCAAAATTGCAAGAAATACTCCAAAGAAAAAACAA
AATGGATGAAGAGAAGCGACACCAGATTCAATCTTGCTCCGTTTCTACTG
TACATACGGAAAGTGAAAAATTGGAATTATTAAAATCCAATTGTTGGGAA
ATAATGGATAACATTTCTTTTATTCAGAAGGAAACAAAACAGAAAATGTG
TTCTCGGTCAACTGTCACTCATCTTACAGAAGCAGTTCAGGACATTGGAA
TCGAAGTAGAAAAGTTGAATTATCACCGAAGTCAATTAGAATCTGAAGTC
GGCCAAATGAGAGAGCAGCTTAGGAATTTACTAGAATCTGTAGACATTCG
TGAGGAGGATTACAAGTTAATAGATTCTGTTATGGATGGGTCGGCAAATT
TCCCATTGCAGGATCATGTGGAATCAAACAAATGGAATTGGTACATTTCA
TGAAGCACTGTTTTCTGAGTATTAATTTCATG
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protein sequence of SMED30025664-orf-1

>SMED30025664-orf-1 ID=SMED30025664-orf-1|Name=SMED30025664-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=477bp
MTQEIPLKNILNKNIEDHKAEIKFIAQSSLNGFKSQNKLENGVKWKTSIV
KTKTKLKSEKSIPFHKSQAQEKDDFTNRLFLFSLGPDEVVDKPETNKQIK
PPILLKSLNKVSRVKEMKLDNKKPISPTYKETVCNMAVSAETFNFSSPRF
PVRDNLRNDEINNEEIKELLPHYIHPSFKTRYVELLRFREENIREKDEME
KEVLAGTNLVKHLEKRLKKKLLNIEKLRHTPNFHRLQIYTEIFDDLISES
WMFGSIFSMIKSAYDSEVERVLEMPVSQKELLESEMDQLRQSIFNKEKLE
NLQSEIVQVEEAIKIAIKTDEEFDNLLIDEEAKLQEILQRKNKMDEEKRH
QIQSCSVSTVHTESEKLELLKSNCWEIMDNISFIQKETKQKMCSRSTVTH
LTEAVQDIGIEVEKLNYHRSQLESEVGQMREQLRNLLESVDIREEDYKLI
DSVMDGSANFPLQDHVESNKWNWYIS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR032755TSNAXIP1_N
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 210..230
NoneNo IPR availableCOILSCoilCoilcoord: 279..319
NoneNo IPR availableCOILSCoilCoilcoord: 412..439
NoneNo IPR availableCOILSCoilCoilcoord: 327..347
NoneNo IPR availablePANTHERPTHR34916FAMILY NOT NAMEDcoord: 3..453
IPR032755Translin-associated factor X-interacting protein 1, N-terminalPFAMPF15739TSNAXIP1_Ncoord: 214..315
e-value: 6.8E-9
score: 36.1