SMED30025657

Overview
NameSMED30025657
Smed IDSMED30025657
Length (bp)1427
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025657 (SMED30025657) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025657 (SMED30025657) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025657 (SMED30025657) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025657 (SMED30025657) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025657

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30025657h1SMcG0009496 Contig38270uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025657h1SMcG0009496 Contig38270newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025657h1SMcG0009496 Contig39437uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025657h1SMcG0009496 Contig39437newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30025657h1SMcG0009496 dd_Smed_v4_3927_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025657h1SMcG0009496 dd_Smed_v4_3927_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30025657h1SMcG0009496 dd_Smed_v4_3927_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025657h1SMcG0009496 dd_Smed_v4_3927_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025657h1SMcG0017191 Contig39437uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025657h1SMcG0017191 Contig39437newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30025657h1SMcG0009496 Contig24429newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30025657h1SMcG0009496 Contig24429newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30025657h1SMcG0009496 Contig24429newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
cholinergic neuronSMED30025657h1SMcG0009496 dd_Smed_v6_3927_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025657h1SMcG0009496 dd_Smed_v4_3927_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025657 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001786:200..3838 -7033
Homology
[Close]
BLAST of SMED30025657 vs. TrEMBL
Match: A0A183R359 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 9.893e-8
Identity = 50/119 (42.02%), Postives = 76/119 (63.87%), Query Frame = -2 
Query: 1001 MNGD-NDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALP-PLIKAVVNRNDAAFKNKRGFAGI 1351
            MNGD + +  Q+LS + SLT+++PELPLP P     S ++LL++ R+S +A   L    + LV  L+ AL S  TDNI+FKEG A++ +D + LP PL+ ++  +    F+ KRGF G+
Sbjct:    1 MNGDISRIPIQSLSAVVSLTDIIPELPLPAPLHAGSSVETLLHDSRISIDAFQCLRFGHDRLVGCLSEALGSVCTDNIDFKEGFASDIIDPHLLPEPLVSSL--KKLPNFEQKRGFCGL 117
[Close]
BLAST of SMED30025657 vs. TrEMBL
Match: A0A5J4P2E1 (Nipped-B protein (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0012224 PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.700e-7
Identity = 58/124 (46.77%), Postives = 84/124 (67.74%), Query Frame = -2 
Query:  983 MNGD-NDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNF 1351
            MNGD + +  Q+L  + SLT++LPELPLP P Q   S+ SLL+EP L +  H +L S  E+L ++LT AL +  TDNI+FK+G A+++VD N LP L+  V+  N A F+++RGF G+   P+F
Sbjct:    1 MNGDISRIPIQSLGAVVSLTDILPELPLPAPLQTGSSTDSLLWEPELPDNVHHFLQSRNEHLTNALTGALETVYTDNIDFKDGFASDSVDLNVLPNLLDNVLVHNHAIFQHRRGFFGL---PSF 121
[Close]
BLAST of SMED30025657 vs. TrEMBL
Match: A0A3R7H1L7 (Nipped-B protein OS=Clonorchis sinensis OX=79923 GN=NIPBL PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.771e-7
Identity = 53/118 (44.92%), Postives = 85/118 (72.03%), Query Frame = -2 
Query: 1001 MNGD-NDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGI 1351
            MNGD + +  QTL  + SLT++LPELPLP P Q+  SS++LL++P+L++    +L+S+   L ++L+ AL +T TDNI+FK+G +++++D N LP L+ +V+  N A  +NKRGF G+
Sbjct:  569 MNGDISRIPIQTLGAVVSLTDILPELPLPAPLQSGPSSKTLLWDPQLTDSTRKYLNSQNSGLTTALSDALENTTTDNIDFKDGLSSDSIDLNGLPGLLTSVLVHNYAVLQNKRGFLGL 686
[Close]
BLAST of SMED30025657 vs. TrEMBL
Match: A0A4S2M6N9 (Nipped-B protein OS=Opisthorchis felineus OX=147828 GN=CRM22_003588 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.439e-7
Identity = 53/118 (44.92%), Postives = 85/118 (72.03%), Query Frame = -2 
Query: 1001 MNGD-NDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGI 1351
            MNGD + +  QTL  + SLT++LPELPLP P Q+  SS++LL++P+L++    +L+S+   L ++L+ AL +T TDNI+FK+G +++++D N LP L+ +V+  N A  +NKRGF G+
Sbjct:    1 MNGDISRIPIQTLGAVVSLTDILPELPLPAPLQSGPSSKTLLWDPQLTDSTRKYLNSQNSGLTTALSDALENTTTDNIDFKDGLSSDSIDLNGLPGLLTSVLVHNYAVLQNKRGFLGL 118
[Close]
BLAST of SMED30025657 vs. TrEMBL
Match: A0A1S8WJT0 (Nipped-B protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_09512 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.188e-6
Identity = 52/118 (44.07%), Postives = 85/118 (72.03%), Query Frame = -2 
Query: 1001 MNGD-NDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGI 1351
            MNGD + +  QTL  + SLT++LPELPLP P Q+  SS++LL++P+L++    +L+S+   L ++L+ AL +T TDNI+FK+G +++++D + LP L+ +V+  N A  +NKRGF G+
Sbjct:    1 MNGDISRIPIQTLGAVVSLTDILPELPLPAPLQSGPSSKTLLWDPQLTDSTRKYLNSQNSGLTTALSDALENTTTDNIDFKDGLSSDSIDLSGLPGLLTSVLMHNYAVLQNKRGFLGL 118
[Close]
BLAST of SMED30025657 vs. Planmine SMEST
Match: SMESG000051020.1 (SMESG000051020.1)

HSP 1 Score: 427.943 bits (1099), Expect = 3.808e-134
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = -2 
Query:  563 MLIMNGDNDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANXXXXXXXXXXXXXXXXXXXSNDEENTQYSIVKTCPDSQKLVF 1360
            MLIMNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLV 
Sbjct:    1 MLIMNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLVL 266

HSP 2 Score: 303.523 bits (776), Expect = 5.921e-91
Identity = 176/201 (87.56%), Postives = 179/201 (89.05%), Query Frame = -3 
Query:    1 ALSKLVPILRNLFLSLIKSPXXXXXXXXXXXXXXXXXXXDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDRVSQCSTSSLVISSSQEPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 603
            ++ K  P  + L LSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR               EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE
Sbjct:  253 SIVKTCPDSQKLVLSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR---------------EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 438
[Close]
BLAST of SMED30025657 vs. Planmine SMEST
Match: SMESG000051020.1 (SMESG000051020.1)

HSP 1 Score: 427.943 bits (1099), Expect = 4.163e-134
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = -2 
Query:  563 MLIMNGDNDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANXXXXXXXXXXXXXXXXXXXSNDEENTQYSIVKTCPDSQKLVF 1360
            MLIMNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLV 
Sbjct:    1 MLIMNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLVL 266

HSP 2 Score: 303.523 bits (776), Expect = 6.776e-91
Identity = 176/201 (87.56%), Postives = 179/201 (89.05%), Query Frame = -3 
Query:    1 ALSKLVPILRNLFLSLIKSPXXXXXXXXXXXXXXXXXXXDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDRVSQCSTSSLVISSSQEPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 603
            ++ K  P  + L LSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR               EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE
Sbjct:  253 SIVKTCPDSQKLVLSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR---------------EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 438
[Close]
BLAST of SMED30025657 vs. Planmine SMEST
Match: SMESG000051020.1 (SMESG000051020.1)

HSP 1 Score: 422.165 bits (1084), Expect = 4.246e-132
Identity = 262/263 (99.62%), Postives = 262/263 (99.62%), Query Frame = -2 
Query:  563 MNGDNDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANXXXXXXXXXXXXXXXXXXXSNDEENTQYSIVKTCPDSQKLVF 1351
            MNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLV 
Sbjct:    1 MNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLVL 263

HSP 2 Score: 303.523 bits (776), Expect = 6.710e-91
Identity = 176/201 (87.56%), Postives = 179/201 (89.05%), Query Frame = -3 
Query:    1 ALSKLVPILRNLFLSLIKSPXXXXXXXXXXXXXXXXXXXDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDRVSQCSTSSLVISSSQEPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 603
            ++ K  P  + L LSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR               EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE
Sbjct:  250 SIVKTCPDSQKLVLSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR---------------EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 435
[Close]
BLAST of SMED30025657 vs. Planmine SMEST
Match: SMESG000051020.1 (SMESG000051020.1)

HSP 1 Score: 422.165 bits (1084), Expect = 4.363e-132
Identity = 262/263 (99.62%), Postives = 262/263 (99.62%), Query Frame = -2 
Query:  563 MNGDNDLAFQTLSGIYSLTNVLPEXXXXXXXXXXXXXXXXLYEPRLSEEAHXXXXXXXXXXXXXLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANXXXXXXXXXXXXXXXXXXXSNDEENTQYSIVKTCPDSQKLVF 1351
            MNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLV 
Sbjct:    1 MNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLSEEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLIKAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNYHLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPPTSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENTQYSIVKTCPDSQKLVL 263

HSP 2 Score: 303.523 bits (776), Expect = 6.453e-91
Identity = 176/201 (87.56%), Postives = 179/201 (89.05%), Query Frame = -3 
Query:    1 ALSKLVPILRNLFLSLIKSPXXXXXXXXXXXXXXXXXXXDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDRVSQCSTSSLVISSSQEPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 603
            ++ K  P  + L LSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR               EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE
Sbjct:  250 SIVKTCPDSQKLVLSLIKSPDDIKKIKKKKKKKDKYKDKDRNRERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLAPVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTLGTPSTTLDR---------------EPDHHHMWNCSVYLTKADDQFDLSMPIDITGNKNE 435
The following BLAST results are available for this feature:
BLAST of SMED30025657 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183R3599.893e-842.02Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A5J4P2E11.700e-746.77Nipped-B protein (Fragment) OS=Paragonimus westerm... [more]
A0A3R7H1L71.771e-744.92Nipped-B protein OS=Clonorchis sinensis OX=79923 G... [more]
A0A4S2M6N94.439e-744.92Nipped-B protein OS=Opisthorchis felineus OX=14782... [more]
A0A1S8WJT01.188e-644.07Nipped-B protein (Fragment) OS=Opisthorchis viverr... [more]
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BLAST of SMED30025657 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025657 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000051020.13.808e-13499.62SMESG000051020.1[more]
SMESG000051020.14.163e-13499.62SMESG000051020.1[more]
SMESG000051020.14.246e-13299.62SMESG000051020.1[more]
SMESG000051020.14.363e-13299.62SMESG000051020.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025657 ID=SMED30025657|Name=SMED30025657|organism=Schmidtea mediterranea sexual|type=transcript|length=1427bp
CTCGTTCTTGTTGCCAGTGATGTCGATCGGCATCGACAGATCAAATTGGT
CGTCGGCCTTTGTCAGATAGACTGAACAGTTCCACATGTGATGATGATCA
GGTTCCTGTGAACTGGATATTACTAGCGATGAAGTGGAACATTGACTAAC
TCGATCCAGTGTCGTGGAGGGAGTTCCCAGCGTTCCGGCGGACATCGATG
TCGGACAATGCTTAGATAAGGACAATGGAGACAGATTTTCATTGAATTGA
TTGGATGATTCTTCTACATTGAATGATTGGTTGGTTTCGATTGCGTTGGT
GACGATTCCTGAGCTGGCTACGACTGGGGCTAGAGATTTGCCGCTGTTGT
CATTGTTGATGGTCAATGGAATTGTACTGGATATGATCTGAGCATTGTCA
CCATTATTAGACAGCTTTATGTTGAGTTTTATTGGCTCTTCGTTTTCTTT
ACATATCGATTTATGTTTCCTCTCTCGGTTTCGATCTTTATCTTTGTATT
TATCTTTCTTTTTCTTCTTTTTTTTAATTTTTTTTATATCATCAGGAGAT
TTAATCAATGATAAAAACAAGTTTCTGAGAATCGGGACAAGTTTTGACAA
TGCTATATTGAGTGTTTTCCTCATCATTGGAATGTCTCGAATCTTTGTGT
TTTTTTCTTTTCTTTTTCTTTTTTCGTTCTGATTTTTTGTTCGCTTCGGA
ATCTTTATCTATTTTGATTATAAGAGTTTCTTTATTAATGCAAGAGTTCA
TTTTGCTGGTTGGAGGCTTATTTTCAATCTGAATTGTGTTTGAAATAAAT
TGGAATTCAGGATAGGAATAGTGAATTGCTTCCAATGCGTTGTCAGGTGT
TTCATTATTGATTTTGTTTATTGGCTGTTCAGGAGATGGAGTCATAACAT
CTGACAAATGGTAATTTGTTGATAAGCCATAATTCATACTCTGAAAATGA
GCTTGTGATTCATTAAGACTGCCCCAATTCATAAAATTCGGTTGAGTAAA
TATTCCAGCAAATCCTCGTTTGTTTTTAAAAGCTGCATCATTTCTATTGA
CAACAGCTTTAATTAGAGGTGGCAAAGCATTATGATCAACTGTTTCGGTT
GCTGATCCTTCTTTAAATTCAATGTTATCAGTGTTTGTACTAGCCAGAGC
CATAGTTAACGAAGAAACTAAATTTTCTTCTTCAGAACTAAGCCAACTAT
GAGCTTCTTCTGAAAGGCGAGGTTCATAAAGCAAACTTTGGCTACTTAAA
GAATTTTGAGAAGGCGTAGGAAGAGGTAATTCCGGTAGCACATTAGTTAA
GCTATATATTCCTGATAAAGTTTGAAAGGCTAGATCATTATCACCATTCA
TTATTAACATGGGCTAAAATTAAGTTTATAAATGCTTTAATATCATAATT
TATAGCAAAATTAAAATAAATAAATTG
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protein sequence of SMED30025657-orf-1

>SMED30025657-orf-1 ID=SMED30025657-orf-1|Name=SMED30025657-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp
MMRKTLNIALSKLVPILRNLFLSLIKSPDDIKKIKKKKKKKDKYKDKDRN
RERKHKSICKENEEPIKLNIKLSNNGDNAQIISSTIPLTINNDNSGKSLA
PVVASSGIVTNAIETNQSFNVEESSNQFNENLSPLSLSKHCPTSMSAGTL
GTPSTTLDRVSQCSTSSLVISSSQEPDHHHMWNCSVYLTKADDQFDLSMP
IDITGNKNE
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protein sequence of SMED30025657-orf-2

>SMED30025657-orf-2 ID=SMED30025657-orf-2|Name=SMED30025657-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=270bp
MLIMNGDNDLAFQTLSGIYSLTNVLPELPLPTPSQNSLSSQSLLYEPRLS
EEAHSWLSSEEENLVSSLTMALASTNTDNIEFKEGSATETVDHNALPPLI
KAVVNRNDAAFKNKRGFAGIFTQPNFMNWGSLNESQAHFQSMNYGLSTNY
HLSDVMTPSPEQPINKINNETPDNALEAIHYSYPEFQFISNTIQIENKPP
TSKMNSCINKETLIIKIDKDSEANKKSERKKKKKRKKHKDSRHSNDEENT
QYSIVKTCPDSQKLVFIID*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000463cholinergic neuron
Vocabulary: INTERPRO
TermDefinition
IPR033031SCC2/Nipped-B
Vocabulary: molecular function
TermDefinition
GO:0003682chromatin binding
Vocabulary: biological process
TermDefinition
GO:0010468regulation of gene expression
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..254
NoneNo IPR availablePANTHERPTHR21704:SF18NIPPED-B-LIKE PROTEINcoord: 6..251
IPR033031SCC2/Nipped-B familyPANTHERPTHR21704NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATEDcoord: 6..251