Enkurin domain-containing protein

Overview
NameEnkurin domain-containing protein
Smed IDSMED30025629
Length (bp)1135
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Enkurin domain-containing protein (SMED30025629) t-SNE clustered cells

Violin plots show distribution of expression levels for Enkurin domain-containing protein (SMED30025629) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Enkurin domain-containing protein (SMED30025629) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Enkurin domain-containing protein (SMED30025629) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30025629

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30025629SMESG000020205.1 SmedASXL_015074SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30025629SMESG000020205.1 dd_Smed_v4_12663_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025629SMESG000020205.1 dd_Smed_v4_12663_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30025629SMESG000020205.1 dd_Smed_v6_12663_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30025629SMESG000020205.1 dd_Smed_v4_12663_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Enkurin domain-containing protein vs. TrEMBL
Match: A0A267H2Y4 (Enkurin domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034090g4 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.368e-12
Identity = 65/204 (31.86%), Postives = 108/204 (52.94%), Query Frame = 1 
Query:  514 KQQLAIQNQTFLKAHENSGPPLDINKYP--PIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQ---SER--YRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKE-------RTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            +QQ     + +L+AHE +GP L+ + +P  P ++     +   RVSVP   SA +++  RR +NY+RANA A     S R   R  + +A+S    D+S YR GE+P YL   + + E   +       +       R L+ LE++  L  L  + + +  ELN LP+  +T+R    K  +E++L E+ + I I+++P V+VK
Sbjct:   14 RQQRRAPKREYLRAHEKTGPDLEADDHPVPPFRR----TAPLDRVSVPRASSANDLRLIRRSVNYVRANANAAAAAGSRRPLGRSGTATAMSKSANDLSGYRRGELPAYLVRRRREFEAAHAAEVAARPDPDQPPGHRRLSDLERRKTLALLTENHQLLLAELNRLPVRSDTVRLVCIKSDIERKLAELEEAIKIFSRPKVFVK 213
[Close]
BLAST of Enkurin domain-containing protein vs. TrEMBL
Match: A0A267E8P4 (Enkurin domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034090g1 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.270e-12
Identity = 65/204 (31.86%), Postives = 108/204 (52.94%), Query Frame = 1 
Query:  514 KQQLAIQNQTFLKAHENSGPPLDINKYP--PIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQ---SER--YRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKE-------RTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            +QQ     + +L+AHE +GP L+ + +P  P ++     +   RVSVP   SA +++  RR +NY+RANA A     S R   R  + +A+S    D+S YR GE+P YL   + + E   +       +       R L+ LE++  L  L  + + +  ELN LP+  +T+R    K  +E++L E+ + I I+++P V+VK
Sbjct:   14 RQQRRAPKREYLRAHEKTGPDLEADDHPVPPFRR----TAPLDRVSVPRASSANDLRLIRRSVNYVRANANAAAAAGSRRPLGRSGTATAMSKSANDLSGYRRGELPAYLVRRRREFEAAHAAEVAARPDPDQPPGHRRLSDLERRKTLALLTENHQLLLAELNRLPVRSDTVRLVRIKSDIERKLAELEEAIKIFSRPKVFVK 213
[Close]
BLAST of Enkurin domain-containing protein vs. TrEMBL
Match: A0A267DVQ2 (Enkurin domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034090g2 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.484e-12
Identity = 66/204 (32.35%), Postives = 106/204 (51.96%), Query Frame = 1 
Query:  514 KQQLAIQNQTFLKAHENSGPPL--DINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQ---SER--YRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKE-------RTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            +QQ     + +L+AHE +GP L  D    PP ++    +    RVSVP   SA +++  RR +NY+RANA A     S R   R  + +A+S    D+S YR GE+P YL   + + E   +       +       R L+ LE++  L  L  + + +  ELN LP+  +T+R    K  +E++L E+ + I I+++P V+VK
Sbjct:   14 RQQRRAPKREYLRAHEKTGPDLEADDRPVPPFRRTAPLD----RVSVPRASSASDLRLIRRSVNYVRANANAAAAAGSRRPLGRSGTATAMSKSANDLSGYRRGELPAYLVRRRREFEAAHAAEVAARPDPDQPPGHRRLSDLERRKTLALLTENHQLLLAELNRLPVRSDTVRLVRIKSDIERKLAELEEAIKIFSRPKVFVK 213
[Close]
BLAST of Enkurin domain-containing protein vs. TrEMBL
Match: A0A433SSV0 (Enkurin domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_019894 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.743e-12
Identity = 63/212 (29.72%), Postives = 107/212 (50.47%), Query Frame = 1 
Query:  487 NQVKDDTIIKQQLAIQNQTFLKAHENSGPPLDINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAY-AIQSERYRVNSKSAVST---RQIDM-SNYRNGEVPKYLK----TFKNKSEEKASRN----FVVSKERTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            +++K D    Q     +  FL+AH  +GPP+ +   P   +++        +SVP   SA +VK  R   ++I+ N   A +S   R  S +++     RQ ++ S+++ GEVP YL+    +++ + EE+  RN     +    R L   E+   L  L      V G++  LPI  +T+R R  +  LEKQL EI + I I+++P V+V+
Sbjct:   52 SKIKQDVHKPQTPRPGSANFLRAHSRAGPPVKLESRPCTPEVS-------ELSVPLASSAGDVKLVRNNFDFIKVNGINARRSATQRPPSAASIDNYKRRQEELLSHHQFGEVPNYLRKRNQSWREEQEERG-RNTPDPAMPPNHRQLPETERTETLNLLTQKQTDVIGQIQRLPIGADTMRVRQHRQSLEKQLGEIEEAIKIFSRPKVFVR 255
[Close]
BLAST of Enkurin domain-containing protein vs. TrEMBL
Match: A0A3B3VSV3 (Enkurin domain containing 1 OS=Poecilia latipinna OX=48699 GN=ENKD1 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.379e-12
Identity = 92/346 (26.59%), Postives = 146/346 (42.20%), Query Frame = 1 
Query:  106 LHCGPITPSNIINDPLYIQELVVRKP--KPRIRIEDKWIKDINEKGSVGILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKIPFDDIYRKPNDKKFILEKEFHTVNVTETENGHFDTTVQPNQVKDDTIIKQQLAI-----QNQTFLKAHENSGPPLDINKYPP------IQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERYRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFK---NKSEEKASRNF----VVSKERTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            LH GP+ P  +    LY      R P  +PR+     +I +  +KG VG L  L GL                    T +P  K   H    D++ R    ++   E+E   ++          T+ +   V    + + +++I     Q QTFLKAH   G     +  PP       Q+    +S KV          +++  Q   +++++ NA A +    R  S+S  + R   + +   G+VP+YL+  K    K EE+  RN     V      L   E+Q  LK LK     +  EL SLP+  + +  RTR+ QL+ +L EI + I I+++  VYVK
Sbjct:   43 LHLGPLAPDPV----LYPDSYSARSPSRRPRVGPNATYILERGQKGVVGDLLRLDGLS------------------ITPVPKQKQKVHDFSKDNVRRLREIQRRCKEQEAERIHAGPVPVKALWTSSKYQNVPSRVMAQLEVSIPPVKAQCQTFLKAHSKGG-----SSAPPRPSPVAFQRPASSSSSKV----------LDLMVQGHTVDFVKHNARAARKTTLR-RSQSLTNLRDKPVPSAVKGQVPQYLEERKKQWQKEEEERKRNAPDPRVPPGHMMLPDGERQETLKSLKDSHGSLVSELLSLPLRSDGLSVRTRRAQLDCKLSEIEEAIKIFSRDRVYVK 350
[Close]
BLAST of Enkurin domain-containing protein vs. Ensembl Nematostella
Match: EDO36286 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ21])

HSP 1 Score: 53.5286 bits (127), Expect = 5.347e-8
Identity = 61/202 (30.20%), Postives = 97/202 (48.02%), Query Frame = 1 
Query:  541 TFLKAHENSGPPLDINKYPPIQKINLKNSEKV-----RVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERYRVNSKSAVSTRQIDMSN------YRN---GEVPKYLKTFKN---KSEEKASRNF----VVSKERTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            T+L+AH  +GPP+         K    ++E V     +++VP    A E +  R++ N++  NA    S      S SA++  ++          YR    G VPKYLKT +    K EE    N     +      +   E+   L  L+ + E +  EL  LP+ ++T+R RTRK +LE++L EI   I I+++P V+VK
Sbjct:  139 TYLRAHSRNGPPV---------KDRPSSTEPVIPKEQKLTVPKADIAKERQPVRKERNHVAMNARRAASPGLYRRSPSAMALEELKQKKKAEEELYRTKSYGNVPKYLKTRQQQWKKEEETRIANIPDPSIPPGHTLMQRDERLKTLDALQKNQEELLNELRLLPVRVDTLRVRTRKTELERRLTEIENAIKIFSRPKVFVK 331
[Close]
BLAST of Enkurin domain-containing protein vs. Ensembl Medaka
Match: si:ch211-220d9.3 (enkurin domain containing 1 [Source:NCBI gene;Acc:101155774])

HSP 1 Score: 60.8474 bits (146), Expect = 7.545e-10
Identity = 58/191 (30.37%), Postives = 88/191 (46.07%), Query Frame = 1 
Query:  532 QNQTFLKAHENSGPPLDINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERYRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFK---NKSEEKASRNF---VVSKERTLTGXXXXXXXXXXXXXXXR-VYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            Q Q FLKAH N          P +  +  KNS              ++K Q + IN+IR NA A      R  S+S    R   + +   G+VP+YL+  K    K EE+  RN     +    TL    ++    K   D  R +  ELNSLP+  + + +R+R+  L+ +L EI + I I+++  VY+K
Sbjct:  191 QCQNFLKAHSNGS-----YAAPSVPSLAFKNSC-------NSAQDQDLKVQGQAINFIRHNARAAGKTVTR-RSQSLTDLRDKPVPSAVKGQVPQYLEERKAQWRKEEEERKRNTPDPTIPPGHTLMQDSERQETLKSLKDTHRTLVMELNSLPLKADNLSTRSRRAHLDCRLSEIEEAIKIFSRDKVYIK 368
[Close]
BLAST of Enkurin domain-containing protein vs. Ensembl Medaka
Match: si:ch211-220d9.3 (enkurin domain containing 1 [Source:NCBI gene;Acc:101155774])

HSP 1 Score: 60.4622 bits (145), Expect = 8.592e-10
Identity = 83/338 (24.56%), Postives = 130/338 (38.46%), Query Frame = 1 
Query:  106 LHCGPITPSNIINDPLYIQELVVRKPKPRIRIEDKWIKDINEKGSVGILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKIPFDDIYRKPNDKKFILEKEFHTVNVTETENGHFDTTVQPNQVKDDTIIKQQLAI-----QNQTFLKAHENSGPPLDINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERYRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFK---NKSEEKASRNF---VVSKERTLTGXXXXXXXXXXXXXXXR-VYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            L  GP  P  ++    Y     V   +PRI      I +   KG VG L +L  +                    +  P  K   H    +++ R    +K   E+E               T  +   V    + + + +      Q Q FLKAH N          P +  +  KNS              ++K Q + IN+IR NA A      R  S+S    R   + +   G+VP+YL+  K    K EE+  RN     +    TL    ++    K   D  R +  ELNSLP+  + + +R+R+  L+ +L EI + I I+++  VY+K
Sbjct:   43 LFLGPFAPDPVVFPGCYSSRTAVHH-RPRIGPNAAHILERGRKGVVGELMKLDNVS------------------VSPAPKPKQRVHDFGKENVRRLKEIQKRCKEQEAEKAQARPVPVKALWTAPKYQHVSSRVLAQLESSTPTVKPQCQNFLKAHSNGS-----YAAPSVPSLAFKNSC-------NSAQDQDLKVQGQAINFIRHNARAAGKTVTR-RSQSLTDLRDKPVPSAVKGQVPQYLEERKAQWRKEEEERKRNTPDPTIPPGHTLMQDSERQETLKSLKDTHRTLVMELNSLPLKADNLSTRSRRAHLDCRLSEIEEAIKIFSRDKVYIK 348
[Close]
BLAST of Enkurin domain-containing protein vs. Planmine SMEST
Match: SMESG000020205.1 (SMESG000020205.1)

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 316/330 (95.76%), Postives = 322/330 (97.58%), Query Frame = 1 
Query:   97 MSKLHCGPITPSNIINDPLYIQELVVRKPKPRIRIEDKWIKDINEKGSVGILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKIPFDDIYRKPNDKKFILEKEFHTVNVTETENGHFDTTVQPNQVKDDTIIKQQLAIQNQTFLKAHENSGPPLDINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERYRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKERTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVKD 1086
            MSKLHCGPITPSNIINDPLYIQEL VRKPKPRIR+EDKWIKDINEKGSVGILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKI FDDIYRKPNDKKFILEKEFHTVNVTETENGH D TVQPNQV+DDTIIKQQLAIQN+TFLKAHENSGPPLD NKYP IQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYA+QSERYR  SKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKERTLTGLEKQIIL+KLK+DLERVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVKD
Sbjct:    1 MSKLHCGPITPSNIINDPLYIQELEVRKPKPRIRMEDKWIKDINEKGSVGILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKILFDDIYRKPNDKKFILEKEFHTVNVTETENGHLDPTVQPNQVEDDTIIKQQLAIQNRTFLKAHENSGPPLDTNKYPTIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYALQSERYRKISKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSKERTLTGLEKQIILEKLKIDLERVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVKD 330
[Close]
BLAST of Enkurin domain-containing protein vs. Planmine SMEST
Match: SMESG000081414.1 (SMESG000081414.1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.338e-21
Identity = 67/198 (33.84%), Postives = 119/198 (60.10%), Query Frame = 1 
Query:  535 NQTFLKAHENSGPPLDINKYP--PIQK-INLKNSEKVRVSVPTGKSAIEVKFQRRQINYIRANAYAIQSERY-RVNSKSAVSTRQ---IDMSNYRNGEVPKYLKTFKNKSEEKASRNFVVSK--------ERTLTGXXXXXXXXXXXXXXXRVYGELNSLPISMETIRSRTRKDQLEKQLDEINKCIDIYNKPNVYVK 1083
            N+TFL+AHE +GPPL +N YP  PI++ +N     + ++SVP   +A EV+  RR I+++RANAYA ++ R  R +S  ++   Q    +    + GE+P YL   +N+ +E+  + F+ ++         R ++  E+   L+ LK   + +  EL  LPI  +T++ RT+K ++E++L+E+ + I I++KP V+V+
Sbjct:  172 NKTFLRAHEKTGPPLGLNAYPLTPIRREVN-----ESKISVPKASTAREVQLIRRDIDFVRANAYAAKTSRLKRYSSADSLVINQNVEKEKKTKKPGEIPSYLIKRRNQWKEE-EQLFIQNQPDPEQPPGHRKMSEKERLSTLELLKATEKDMLTELGRLPIRSDTLKLRTKKQEIEQKLNELEEAIKIFSKPKVFVR 363
The following BLAST results are available for this feature:
BLAST of Enkurin domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267H2Y41.368e-1231.86Enkurin domain-containing protein OS=Macrostomum l... [more]
A0A267E8P42.270e-1231.86Enkurin domain-containing protein OS=Macrostomum l... [more]
A0A267DVQ23.484e-1232.35Enkurin domain-containing protein OS=Macrostomum l... [more]
A0A433SSV03.743e-1229.72Enkurin domain-containing protein OS=Elysia chloro... [more]
A0A3B3VSV37.379e-1226.59Enkurin domain containing 1 OS=Poecilia latipinna ... [more]
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO362865.347e-830.20Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Enkurin domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch211-220d9.37.545e-1030.37enkurin domain containing 1 [Source:NCBI gene;Acc:... [more]
si:ch211-220d9.38.592e-1024.56enkurin domain containing 1 [Source:NCBI gene;Acc:... [more]
back to top
BLAST of Enkurin domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000020205.10.000e+095.76SMESG000020205.1[more]
SMESG000081414.11.338e-2133.84SMESG000081414.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025629 ID=SMED30025629|Name=Enkurin domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1135bp
CTATACTTCAAATAGTTACACTTTAATAGTTATAATTTGAAAGTGTAAAT
TTTTTAAAGAGAAATTAATTGGATTATTAATCAATGTGTTGATGAAATGA
GTAAATTACATTGCGGACCAATTACTCCTTCAAATATAATTAATGATCCT
CTATATATACAGGAATTGGTAGTTAGAAAACCCAAGCCCAGAATAAGAAT
TGAAGATAAATGGATAAAAGATATCAATGAAAAGGGCTCAGTGGGAATAC
TTTTTGAGTTACAAGGCTTAGAATCAAAATTTCAAGAATTACAATTTTTA
CAAAGAGAAAGATCAAAAATGCGACAAGCAACAGCAATTCCTTCGTGTAA
AAGTGGAAGACATAAAATTCCATTTGATGATATCTATCGAAAACCAAACG
ATAAAAAGTTTATTCTCGAAAAAGAATTTCATACTGTAAATGTAACTGAA
ACTGAGAATGGTCATTTTGATACAACTGTTCAACCGAATCAAGTTAAAGA
CGACACAATTATCAAACAACAATTAGCCATTCAGAACCAAACATTCCTGA
AAGCACATGAAAATTCGGGCCCTCCTCTTGACATTAATAAATACCCACCA
ATTCAGAAAATTAATTTGAAAAACTCAGAAAAAGTTAGAGTTTCAGTTCC
AACTGGGAAATCAGCTATAGAAGTGAAATTCCAACGACGCCAAATCAATT
ACATTAGAGCAAATGCGTACGCTATACAATCAGAGAGATACAGAGTGAAT
TCAAAATCTGCCGTATCAACCCGACAAATTGATATGAGTAATTACAGGAA
TGGCGAAGTCCCAAAATATCTTAAAACTTTCAAAAACAAATCCGAAGAAA
AGGCGTCAAGAAATTTTGTAGTTTCCAAAGAACGCACTCTCACCGGTTTG
GAAAAACAAATAATTCTTAAAAAATTGAAATTAGATTTGGAAAGAGTTTA
TGGTGAATTGAATTCCCTGCCAATTTCTATGGAAACCATCCGATCTAGAA
CAAGAAAAGATCAGCTAGAGAAGCAGCTTGATGAAATAAATAAATGCATT
GATATATATAACAAACCAAATGTTTATGTAAAGGATTAATTTTTGCTTTT
TTTGTTGTAAATGAAATAATTATATATATATAATA
back to top

protein sequence of SMED30025629-orf-1

>SMED30025629-orf-1 ID=SMED30025629-orf-1|Name=SMED30025629-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=331bp
MSKLHCGPITPSNIINDPLYIQELVVRKPKPRIRIEDKWIKDINEKGSVG
ILFELQGLESKFQELQFLQRERSKMRQATAIPSCKSGRHKIPFDDIYRKP
NDKKFILEKEFHTVNVTETENGHFDTTVQPNQVKDDTIIKQQLAIQNQTF
LKAHENSGPPLDINKYPPIQKINLKNSEKVRVSVPTGKSAIEVKFQRRQI
NYIRANAYAIQSERYRVNSKSAVSTRQIDMSNYRNGEVPKYLKTFKNKSE
EKASRNFVVSKERTLTGLEKQIILKKLKLDLERVYGELNSLPISMETIRS
RTRKDQLEKQLDEINKCIDIYNKPNVYVKD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR027710ENKD1
IPR027012Enkurin_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 58..78
NoneNo IPR availablePANTHERPTHR21490UNCHARACTERIZEDcoord: 147..329
IPR027012Enkurin domainPFAMPF13864Enkurincoord: 236..324
e-value: 4.8E-14
score: 52.6
IPR027012Enkurin domainPROSITEPS51665ENKURINcoord: 233..329
score: 13.13
IPR027710Enkurin domain-containing protein 1PANTHERPTHR21490:SF2ENKURIN DOMAIN-CONTAINING PROTEIN 1coord: 147..329