E3 ubiquitin-protein ligase LRSAM1

Overview
NameE3 ubiquitin-protein ligase LRSAM1
Smed IDSMED30025624
Length (bp)2468
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of E3 ubiquitin-protein ligase LRSAM1 (SMED30025624) t-SNE clustered cells

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase LRSAM1 (SMED30025624) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of E3 ubiquitin-protein ligase LRSAM1 (SMED30025624) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for E3 ubiquitin-protein ligase LRSAM1 (SMED30025624) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025624

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30025624SMESG000046440.1 SMESG000027652.1 dd_Smed_v4_10841_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30025624SMESG000046440.1 SMESG000027652.1 dd_Smed_v4_10841_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30025624SMESG000046440.1 SMESG000027652.1 dd_Smed_v4_10841_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025624SMESG000046440.1 SMESG000027652.1 Contig5038newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025624SMESG000046440.1 SMESG000027652.1 Contig5038uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025624SMESG000005268.1 Contig5038newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025624SMESG000005268.1 Contig5038uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:HGNC Symbol;Acc:HGNC:25135])

HSP 1 Score: 167.162 bits (422), Expect = 1.964e-42
Identity = 181/683 (26.50%), Postives = 357/683 (52.27%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNE-DNAVDTVDFST-------LDYENF-----KRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV------IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLR-------QNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMI--MTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N L  LP S+GNL  L  L +++N++++LP + +    SL  +++S N +   +P  + ++R L   +  ++ + +P +++C  G   IL+ +CK + +   P +      L  +  +N+ D+ D  T       L+++N      KR    +L+ +E E  LE   +       ++  ++     T+ EE++++   +++     +  R++L   +K  E + ++ I++++  N+  +   ++L  +E+E ++   L  I+++E ++LR+++V ++M + L    K    N L ++A+     + V      + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L + + E+E + +  Q+++WLIQYQR L+ KP  L   + G++ ++  LL  ++A  YLP F  ++  LD L + +  D   VG+    L+       Q LLD  R ++ E    + E     A    P    P      +  + +EC +CL  E +++F++CGH+CCC++C   L+ CPLCR +I + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELP-DTVGELRSLRTLNISGN-EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCK----NRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAAR-IQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRI 719
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:HGNC Symbol;Acc:HGNC:25135])

HSP 1 Score: 167.162 bits (422), Expect = 1.964e-42
Identity = 181/683 (26.50%), Postives = 357/683 (52.27%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNE-DNAVDTVDFST-------LDYENF-----KRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV------IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLR-------QNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMI--MTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N L  LP S+GNL  L  L +++N++++LP + +    SL  +++S N +   +P  + ++R L   +  ++ + +P +++C  G   IL+ +CK + +   P +      L  +  +N+ D+ D  T       L+++N      KR    +L+ +E E  LE   +       ++  ++     T+ EE++++   +++     +  R++L   +K  E + ++ I++++  N+  +   ++L  +E+E ++   L  I+++E ++LR+++V ++M + L    K    N L ++A+     + V      + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L + + E+E + +  Q+++WLIQYQR L+ KP  L   + G++ ++  LL  ++A  YLP F  ++  LD L + +  D   VG+    L+       Q LLD  R ++ E    + E     A    P    P      +  + +EC +CL  E +++F++CGH+CCC++C   L+ CPLCR +I + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELP-DTVGELRSLRTLNISGN-EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCK----NRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAAR-IQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRI 719
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:HGNC Symbol;Acc:HGNC:25135])

HSP 1 Score: 167.162 bits (422), Expect = 1.964e-42
Identity = 181/683 (26.50%), Postives = 357/683 (52.27%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNE-DNAVDTVDFST-------LDYENF-----KRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV------IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLR-------QNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMI--MTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N L  LP S+GNL  L  L +++N++++LP + +    SL  +++S N +   +P  + ++R L   +  ++ + +P +++C  G   IL+ +CK + +   P +      L  +  +N+ D+ D  T       L+++N      KR    +L+ +E E  LE   +       ++  ++     T+ EE++++   +++     +  R++L   +K  E + ++ I++++  N+  +   ++L  +E+E ++   L  I+++E ++LR+++V ++M + L    K    N L ++A+     + V      + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L + + E+E + +  Q+++WLIQYQR L+ KP  L   + G++ ++  LL  ++A  YLP F  ++  LD L + +  D   VG+    L+       Q LLD  R ++ E    + E     A    P    P      +  + +EC +CL  E +++F++CGH+CCC++C   L+ CPLCR +I + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELP-DTVGELRSLRTLNISGN-EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCK----NRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAAR-IQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRI 719
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:HGNC Symbol;Acc:HGNC:25135])

HSP 1 Score: 159.073 bits (401), Expect = 7.982e-40
Identity = 177/685 (25.84%), Postives = 343/685 (50.07%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNE-DNAVDTVDFST-------LDYENF-----KRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV------IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDI--RSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLR-------QNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMI--MTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N L  LP S+GNL  L  L +++N++++LP + +    SL  +++S N +   +P  + ++R L   +  ++ + +P +++C  G   IL+ +CK + +   P +      L  +  +N+ D+ D  T       L+++N      KR    +L+ +E E  LE   +       ++  ++     T+ EE++++   +++     +  R++L   +K  E + ++ I++++  N+  +   ++L  +E+E ++   L  I+++E ++LR+++V ++M + L    K    N L ++A+     + V      + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+  R +R  E I                             +E+R  L  +LQ L+++K  RE  L + + E+E + +  Q+++WLIQYQR L+ KP  L   + G++ ++  LL  ++A  YLP F  ++  LD L + +  D   VG+    L+       Q LLD  R ++ E    + E     A    P    P      +  + +EC +CL  E +++F++CGH+CCC++C   L+ CPLCR +I + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELP-DTVGELRSLRTLNISGN-EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCK----NRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQEMI-----------------------------SEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAAR-IQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRI 692
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Match: BIRC2 (baculoviral IAP repeat containing 2 [Source:HGNC Symbol;Acc:HGNC:590])

HSP 1 Score: 55.4546 bits (132), Expect = 3.782e-7
Identity = 17/47 (36.17%), Postives = 34/47 (72.34%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C+++E  ++F+ CGH+  C++C+ +L++CP+CR  IK  ++
Sbjct:  568 ERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVR 614
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Celegans
Match: K11D12.9 (pep chromosome:WBcel235:V:5042408:5046203:-1 gene:WBGene00019649.1 transcript:K11D12.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K11D12.9)

HSP 1 Score: 52.373 bits (124), Expect = 1.023e-6
Identity = 20/50 (40.00%), Postives = 30/50 (60.00%), Query Frame = 2 
Query: 2234 TECCICLNEETKILFMDCGHICCCEKC--SNNLKECPLCRNEIKKIIKLM 2377
             ECCICL  +  I+FM C H+  C  C  +++ +ECP CR+ I+  I + 
Sbjct:  350 AECCICLATKPSIVFMPCRHLITCSGCYDASDFRECPTCRSTIENSITVF 399
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Match: Diap2 (gene:FBgn0015247 transcript:FBtr0087297)

HSP 1 Score: 55.4546 bits (132), Expect = 1.551e-7
Identity = 17/44 (38.64%), Postives = 31/44 (70.45%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            C +CL+EE  ++F+ CGH+  C +C+ ++  CP+CR +IK  ++
Sbjct:  451 CKVCLDEEVGVVFLPCGHLATCNQCAPSVANCPMCRADIKGFVR 494
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Match: Diap2 (gene:FBgn0015247 transcript:FBtr0087296)

HSP 1 Score: 55.4546 bits (132), Expect = 1.551e-7
Identity = 17/44 (38.64%), Postives = 31/44 (70.45%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            C +CL+EE  ++F+ CGH+  C +C+ ++  CP+CR +IK  ++
Sbjct:  451 CKVCLDEEVGVVFLPCGHLATCNQCAPSVANCPMCRADIKGFVR 494
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0075520)

HSP 1 Score: 51.6026 bits (122), Expect = 3.662e-6
Identity = 18/46 (39.13%), Postives = 27/46 (58.70%), Query Frame = 2 
Query: 2237 ECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            EC +C +    + F  CGH+  CE CS  +K+C LCR +I +I+  
Sbjct: 1016 ECLVCSDRRAAVFFRPCGHMVACEHCSALMKKCVLCRTQIDEILSF 1061
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Match: Diap1 (gene:FBgn0260635 transcript:FBtr0075500)

HSP 1 Score: 50.8322 bits (120), Expect = 3.828e-6
Identity = 16/45 (35.56%), Postives = 27/45 (60.00%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            C IC   E    F+ CGH+  C KC++++ +CPLCR     ++++
Sbjct:  391 CKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRV 435
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Match: Diap1 (gene:FBgn0260635 transcript:FBtr0333618)

HSP 1 Score: 50.8322 bits (120), Expect = 3.828e-6
Identity = 16/45 (35.56%), Postives = 27/45 (60.00%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            C IC   E    F+ CGH+  C KC++++ +CPLCR     ++++
Sbjct:  391 CKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRV 435
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:ZFIN;Acc:ZDB-GENE-060526-97])

HSP 1 Score: 203.371 bits (516), Expect = 7.027e-55
Identity = 187/691 (27.06%), Postives = 341/691 (49.35%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNA-VDTVDFST-------LDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQ---------LMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAF-------ARSLN------DKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQ--NKKICEKEEAIATMNE------------PNCSLPQTMIMTHR-ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            VP   FS+CKVL+KK L L+ N ++ L   G  L  L  L  L L  N ++ LPD I ++  LQVLN   N +  LP+++G LL+L  L ++ N +  LP+++   +S    +D+SEN     +P  + ++R L      +  + FP   +C  G  E+ + +C    +   P +      L ++     +D VD          +DYE  K  +         +LE ++  +     +  H +++  N+  +E+  ++ ++ +  LE  + ++Q         +++ V+ AE+   + I +++  N+  +     L  +E + ++  HL  I+++E  +LRK+EV  +M   L     +    E +E           +SL+      D+V+     ++ K +S++  E    E  QK AF+ LQ+QKD     +   I  +E+EL+ L++ E K+R    +     L+++R  L ++LQ L++QK  RE  L   ++E+EK+ + +Q ++W+IQYQR LD KP  L   ++ +D ++  LL +++A  YLP    ++   + L R T  D + +G+    +++ LL   R   +     K + E EEA  +               P+  L  T     R  +EC +C+  E++++F+ CGH+CCC+ CS+ L+ CPLCR  I + +++
Sbjct:   43 VPSCAFSMCKVLQKKVLILHTNDLRSLVPKGCCLGALATLKVLDLHENKLTSLPDEIGQLSSLQVLNAEKNQIKQLPDTIGGLLHLQTLNVKGNCLTVLPVSVGRMSSL-RTLDISEN-SIRELPKELASVRTLESLILDAQVMSFPPASVCTGGTEEVQRYLCSEMGLEYCPPSQYLLPVLEHDGGKQEMDCVDGEEIVWQNKFMDYEKRKEQK---------QLEKLIFEKDLEEKQREHAKLLVLNSSLKEDVLLSVKLEQERLELGVSQQQKAQEAERLKVLDKVRQAESAVMSRISDLLLDNKRQEKSAAFLQALEKDRIRMEHLTTITQEEANSLRKKEVADAMQRMLSESCSVRLLQEAREAKTKFLVSETCKSLDNMDKKFDQVLSLQQLDKSKAISQILQE----EEMQKAAFEALQLQKDSVHAYIRNQIKLIEAELMQLTKLEVKRRNLDTENLQEELSDQRTALTDLLQQLLKQKDQREEELRLVLMEIEKKSESNQQNYWMIQYQRLLDAKPLSLRMQEAAVDRDLGNLLCKLSAQHYLPIIAHHRITAEALRRMTAKDLRKLGINEVGVQKALLHWARDRTLSDPLPKTLKEMEEAGPSAQSVPLPHQLTPPLTPSAPLTPTTPNPDRFNSECVVCMELESQVIFLPCGHVCCCQTCSDALQSCPLCRGSISQRVRI 718
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:ZFIN;Acc:ZDB-GENE-060526-97])

HSP 1 Score: 201.445 bits (511), Expect = 2.642e-54
Identity = 187/691 (27.06%), Postives = 341/691 (49.35%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNA-VDTVDFST-------LDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQ---------LMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAF-------ARSLN------DKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQ--NKKICEKEEA------------IATMNEPNCSLPQTMIMTHR-ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            VP   FS+CKVL+KK L L+ N ++ L   G  L  L  L  L L  N ++ LPD I ++  LQVLN   N +  LP+++G LL+L  L ++ N +  LP+++   +S    +D+SEN     +P  + ++R L      +  + FP   +C  G  E+ + +C    +   P +      L ++     +D VD          +DYE  K  +         +LE ++  +     +  H +++  N+  +E+  ++ ++ +  LE  + ++Q         +++ V+ AE+   + I +++  N+  +     L  +E + ++  HL  I+++E  +LRK+EV  +M   L     +    E +E           +SL+      D+V+     ++ K +S++  E    E  QK AF+ LQ+QKD     +   I  +E+EL+ L++ E K+R    +     L+++R  L ++LQ L++QK  RE  L   ++E+EK+ + +Q ++W+IQYQR LD KP  L   ++ +D ++  LL +++A  YLP    ++   + L R T  D + +G+    +++ LL   R   +     K + E EEA            +     P+  L  T     R  +EC +C+  E++++F+ CGH+CCC+ CS+ L+ CPLCR  I + +++
Sbjct:   43 VPSCAFSMCKVLQKKVLILHTNDLRSLVPKGCCLGALATLKVLDLHENKLTSLPDEIGQLSSLQVLNAEKNQIKQLPDTIGGLLHLQTLNVKGNCLTVLPVSVGRMSSL-RTLDISEN-SIRELPKELASVRTLESLILDAQVMSFPPASVCTGGTEEVQRYLCSDMGLEYCPPSQYLLPVLEHDGGKQEMDCVDGEEIVWQNKFMDYEKRKEQK---------QLEKLIFEKDLEEKQREHAKLLVLNSSLKEDVLLSVKLEQERLELGVSQQQKAQEAERLKVLDKVRQAESAVMSRISDLLLDNKRQEKSAAFLQALEKDRIRMEHLTTITQEEANSLRKKEVADAMQRMLSESCSVRLLQEAREAKTKFLVSETCKSLDNMDKKFDQVLSLQQLDKSKAISQILQE----EEMQKAAFEALQLQKDSVHAYIRNQIKLIEAELMQLTKLEVKRRNLDTENLQEELSDQRTALTDLLQQLLKQKDQREEELRLVLMEIEKKSESNQQNYWMIQYQRLLDAKPLSLRMQEAAVDRDLGNLLCKLSAQHYLPIIAHHRITAEALRRMTAKDLRKLGINEVGVQKALLHWARDRTLSDPLPKTLKEMEEAGPFAQSVPLPHQLTPPLTPSAPLTPTTPNPDRFNSECVVCMELESQVIFLPCGHVCCCQTCSDALQSCPLCRGSISQRVRI 718
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:ZFIN;Acc:ZDB-GENE-060526-97])

HSP 1 Score: 197.593 bits (501), Expect = 5.531e-53
Identity = 163/636 (25.63%), Postives = 303/636 (47.64%), Query Frame = 2 
Query:  596 ISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNA-VDTVDFSTLDYENFKRNRLDLLKSIES-ELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQ---------LMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAF-------ARSLN------DKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQ--NKKICEKEEA------------IATMNEPNCSLPQTMIMTHR-ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            I +LPD I  + +LQ LNV  N LT LP S+G +  L                          +D+SEN     +P  + ++R L      +  + FP   +C  G  E+ + +C    +   P +      L ++     +D VD   + ++N     +D  K  E  +LE ++  +     +  H +++  N+  +E+  ++ ++ +  LE  + ++Q         +++ V+ AE+   + I +++  N+  +     L  +E + ++  HL  I+++E  +LRK+EV  +M   L     +    E +E           +SL+      D+V+     ++ K +S++  E    E  QK AF+ LQ+QKD     +   I  +E+EL+ L++ E K+R    +     L+++R  L ++LQ L++QK  RE  L   ++E+EK+ + +Q ++W+IQYQR LD KP  L   ++ +D ++  LL +++A  YLP    ++   + L R T  D + +G+    +++ LL   R   +     K + E EEA            +     P+  L  T     R  +EC +C+  E++++F+ CGH+CCC+ CS+ L+ CPLCR  I + +++
Sbjct:  108 IKQLPDTIGGLLHLQTLNVKGNCLTVLPVSVGRMSSL------------------------RTLDISEN-SIRELPKELASVRTLESLILDAQVMSFPPASVCTGGTEEVQRYLCSDMGLEYCPPSQYLLPVLEHDGGKQEMDCVDGEEIVWQN---KFMDYEKRKEQKQLEKLIFEKDLEEKQREHAKLLVLNSSLKEDVLLSVKLEQERLELGVSQQQKAQEAERLKVLDKVRQAESAVMSRISDLLLDNKRQEKSAAFLQALEKDRIRMEHLTTITQEEANSLRKKEVADAMQRMLSESCSVRLLQEAREAKTKFLVSETCKSLDNMDKKFDQVLSLQQLDKSKAISQILQE----EEMQKAAFEALQLQKDSVHAYIRNQIKLIEAELMQLTKLEVKRRNLDTENLQEELSDQRTALTDLLQQLLKQKDQREEELRLVLMEIEKKSESNQQNYWMIQYQRLLDAKPLSLRMQEAAVDRDLGNLLCKLSAQHYLPIIAHHRITAEALRRMTAKDLRKLGINEVGVQKALLHWARDRTLSDPLPKTLKEMEEAGPFAQSVPLPHQLTPPLTPSAPLTPTTPNPDRFNSECVVCMELESQVIFLPCGHVCCCQTCSDALQSCPLCRGSISQRVRI 711
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Match: mul1b (mitochondrial E3 ubiquitin protein ligase 1b [Source:ZFIN;Acc:ZDB-GENE-050208-236])

HSP 1 Score: 56.9954 bits (136), Expect = 4.749e-8
Identity = 23/50 (46.00%), Postives = 32/50 (64.00%), Query Frame = 2 
Query: 2234 TECCICLNEETKILFMDCGHICCCEKCSNNL---KECPLCRNEIKKIIKL 2374
            T C ICLN +   +F++CGH+C CE C   L   K+CP+CR  I +I+ L
Sbjct:  300 TACTICLNHKRSCVFLECGHVCSCEGCYRALPEPKKCPICRATIDRIVSL 349
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Match: mul1b (mitochondrial E3 ubiquitin protein ligase 1b [Source:ZFIN;Acc:ZDB-GENE-050208-236])

HSP 1 Score: 56.9954 bits (136), Expect = 4.749e-8
Identity = 23/50 (46.00%), Postives = 32/50 (64.00%), Query Frame = 2 
Query: 2234 TECCICLNEETKILFMDCGHICCCEKCSNNL---KECPLCRNEIKKIIKL 2374
            T C ICLN +   +F++CGH+C CE C   L   K+CP+CR  I +I+ L
Sbjct:  300 TACTICLNHKRSCVFLECGHVCSCEGCYRALPEPKKCPICRATIDRIVSL 349
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:100124327])

HSP 1 Score: 226.868 bits (577), Expect = 4.481e-63
Identity = 195/684 (28.51%), Postives = 356/684 (52.05%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTL--------------DYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELEN-----------------QENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTM-------IMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G FS C+VL+KK L +++N +  L     ++ DL+ L  L L  N I+ LP NI ++  L+VLNV  N L  LP+S+G+L  L  L ++ N++  LP + +    SL  +++ EN +   +P ++ N+R L   +  +  + FP   +C +G   I + +CK   I   P +    + L NN  +  DTVD + L              +YE  K  ++      E +L+   + Q     ++  ++     T+ EE+ ++   +NK   +    R++L   +K  E   +  I+ ++  NR  +   ++L  +E E +K  HL  I+++E  NLR++EV ++M + L    K    ++L ++A+     + V    ++     L++M  +L+                  QE  QK AF+ LQ++KD+   ++ E I  +E+EL+ L+Q E K++E   +    ++ E+R+ L ++LQ L++ K  RE  L   +LEM+ + +  Q+++WLIQYQR L+ KP  L   + GL+ E+ +LL  ++A +YLP F  ++  L+ LE+ +  D K +G+    L+  +++  R++   ++  +   E+ +    E  C+LP  M        + +R +EC +C+ +E  ++F+ CGH+CCC  C + L+ CPLCR +I + I++
Sbjct:   44 IPDGAFSTCRVLQKKVLIVHSNFLTQLVPKSCHMSDLSALKVLDLHENQIAALPANIGQLTCLRVLNVERNLLRALPDSIGDLTQLQTLNVKGNKLRVLP-STIGGLRSLRTLNICEN-RLAELPRSLANVRTLETLSLDATAMLFPPPSVCGEGTEAIQRFLCKELGIEYCPPSQYLLSVLENNSCD-TDTVDGALLRSLQEQTDWQNKFSEYEKRKEQKMMEKLEFERQLDLEQREQAQLLLQSNTQKDEILQTVREEQWRLEKGLNKHQQQIQSERQRLQEQLKQMEKGISYRIKNLLEQNRRQKQNSEILKSLEDERIKMEHLMAITQEEADNLRRKEVASAMQQMLAETYK----SKLLQMAYESRRQEMVFQTCSS-----LADMDRKLQQIQHWQQMDQNKAISQILQEEIQKAAFEALQLKKDLMHRQIREQILLIEAELMQLTQLEVKRQEFGNEALQEVIAEQRRALTDMLQQLLKDKKQREEELLAVLLEMDSKSEAKQENYWLIQYQRLLNQKPLSLRLQEEGLEQELVDLLKALSAEKYLPIFAHHRFCLEMLEKMSPADLKKIGVEEVGLQHAIIERAREIVRTRSGNVLTLEKLVP--EEEPCALPSPMEPSAPDTEVANRNSECVVCMEQEAHVIFLPCGHVCCCTNCGDALRTCPLCRRDIGQRIRI 713
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:100124327])

HSP 1 Score: 207.994 bits (528), Expect = 1.778e-56
Identity = 189/663 (28.51%), Postives = 344/663 (51.89%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTL--------------DYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELEN-----QENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKII 2368
            +P G FS C+VL+KK L +++N +  L     ++ DL+ L  L L  N I+ LP NI ++  L+VLNV  N L  LP+S+G+L  L  L ++ N++  LP + +    SL  +++ EN +   +P ++ N+R L   +  +  + FP   +C +G   I + +CK   I   P +    + L NN  +  DTVD + L              +YE  K  ++      E +L+   + Q     ++  ++     T+ EE+ ++   +NK   +    R++L   +K  E   +  I+ ++  NR  +   ++L  +E E +K  HL  I+++E  NLR++EV ++M + L    K    ++L ++A+     + V    ++     L++M  +L+      Q ++ K   Q+LQ   DI         +  ++EL+ L+Q E K++E   +    ++ E+R+ L ++LQ L++ K  RE  L   +LEM+ + +  Q+++WLIQYQR L+ KP  L   + GL+ E+ +LL  ++A +YLP F  ++  L+ LE+ +  D K +G+    L+  +++  R++   ++  +   EE  A  +    S P T +  +R +EC +C+ +E  ++F+ CGH+CCC  C + L+ CPLCR +I + I
Sbjct:   44 IPDGAFSTCRVLQKKVLIVHSNFLTQLVPKSCHMSDLSALKVLDLHENQIAALPANIGQLTCLRVLNVERNLLRALPDSIGDLTQLQTLNVKGNKLRVLP-STIGGLRSLRTLNICEN-RLAELPRSLANVRTLETLSLDATAMLFPPPSVCGEGTEAIQRFLCKELGIEYCPPSQYLLSVLENNSCD-TDTVDGALLRSLQEQTDWQNKFSEYEKRKEQKMMEKLEFERQLDLEQREQAQLLLQSNTQKDEILQTVREEQWRLEKGLNKHQQQIQSERQRLQEQLKQMEKGISYRIKNLLEQNRRQKQNSEILKSLEDE-IKMEHLMAITQEEADNLRRKEVASAMQQMLAETYK----SKLLQMAYESRRQEMVFQTCSS-----LADMDRKLQQIQHWQQMDQNKAISQILQEVSDI-----PRGPHRPKAELMQLTQLEVKRQEFGNEALQEVIAEQRRALTDMLQQLLKDKKQREEELLAVLLEMDSKSEAKQENYWLIQYQRLLNQKPLSLRLQEEGLEQELVDLLKALSAEKYLPIFAHHRFCLEMLEKMSPADLKKIGVEEVGLQHAIIERAREIVRTRSGNVLSMEEPCALPSPMEPSAPDTEV-ANRNSECVVCMEQEAHVIFLPCGHVCCCTNCGDALRTCPLCRRDIGQRI 687
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:100124327])

HSP 1 Score: 204.142 bits (518), Expect = 3.189e-55
Identity = 188/677 (27.77%), Postives = 339/677 (50.07%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTL--------------DYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELEN-----------------QENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G FS C+VL+KK L +++N +  L     ++ DL+ L  L L  N I+ LP NI ++  L+VLNV  N L  LP+S+G+L  L  L ++ N++  LP + +    SL  +++ EN +   +P ++ N+R L   +  +  + FP   +C +G   I + +CK   I   P +    + L NN  +  DTVD + L              +YE  K  ++      E +L+   + Q     ++  ++     T+ EE+ ++   +NK   +    R++L   +K  E   +  I+ ++  NR  +   ++L  +E E +K  HL  I+++E  NLR++EV ++M + L    K    ++L ++A+     + V    ++     L++M  +L+                  QE  QK AF+ LQ++KD+   ++ E                             ++ E+R+ L ++LQ L++ K  RE  L   +LEM+ + +  Q+++WLIQYQR L+ KP  L   + GL+ E+ +LL  ++A +YLP F  ++  L+ LE+ +  D K +G+    L+  +++  R+  I   +K+  +EE  A  +    S P T +  +R +EC +C+ +E  ++F+ CGH+CCC  C + L+ CPLCR +I + I++
Sbjct:   44 IPDGAFSTCRVLQKKVLIVHSNFLTQLVPKSCHMSDLSALKVLDLHENQIAALPANIGQLTCLRVLNVERNLLRALPDSIGDLTQLQTLNVKGNKLRVLP-STIGGLRSLRTLNICEN-RLAELPRSLANVRTLETLSLDATAMLFPPPSVCGEGTEAIQRFLCKELGIEYCPPSQYLLSVLENNSCD-TDTVDGALLRSLQEQTDWQNKFSEYEKRKEQKMMEKLEFERQLDLEQREQAQLLLQSNTQKDEILQTVREEQWRLEKGLNKHQQQIQSERQRLQEQLKQMEKGISYRIKNLLEQNRRQKQNSEILKSLEDERIKMEHLMAITQEEADNLRRKEVASAMQQMLAETYK----SKLLQMAYESRRQEMVFQTCSS-----LADMDRKLQQIQHWQQMDQNKAISQILQEEIQKAAFEALQLKKDLMHRQIREQ---------------------------EVIAEQRRALTDMLQQLLKDKKQREEELLAVLLEMDSKSEAKQENYWLIQYQRLLNQKPLSLRLQEEGLEQELVDLLKALSAEKYLPIFAHHRFCLEMLEKMSPADLKKIGVEEVGLQHAIIERARE--IVPLEKLVPEEEPCALPSPMEPSAPDTEV-ANRNSECVVCMEQEAHVIFLPCGHVCCCTNCGDALRTCPLCRRDIGQRIRI 678
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Match: LRSAM1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:100124327])

HSP 1 Score: 196.052 bits (497), Expect = 1.902e-52
Identity = 162/638 (25.39%), Postives = 302/638 (47.34%), Query Frame = 2 
Query:  596 ISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTL--------------DYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELEN-----------------QENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRET----------ECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +  LPD+I  +  LQ LNV  N L  LP                        + +    SL  +++ EN +   +P ++ N+R L   +  +  + FP   +C +G   I + +CK   I   P +    + L NN  +  DTVD + L              +YE  K  ++      E +L+   + Q     ++  ++     T+ EE+ ++   +NK   +    R++L   +K  E   +  I+ ++  NR  +   ++L  +E E +K  HL  I+++E  NLR++EV ++M + L    K    ++L ++A+     + V    ++     L++M  +L+                  QE  QK AF+ LQ++KD+   ++ E I  +E+EL+ L+Q E K++E   +    ++ E+R+ L ++LQ L++ K  RE  L    LEM+ + +  Q+++WLIQYQR L+ KP  L   + GL+ E+ +LL  ++A +YLP F  ++  L+ LE+ +  D K +G+    L+  +++  R+  I ++ ++  + +         C  P ++ +  +            +CC        ++F+ CGH+CCC  C + L+ CPLCR +I + I++
Sbjct:   92 LRALPDSIGDLTQLQTLNVKGNKLRVLP------------------------STIGGLRSLRTLNICEN-RLAELPRSLANVRTLETLSLDATAMLFPPPSVCGEGTEAIQRFLCKELGIEYCPPSQYLLSVLENNSCD-TDTVDGALLRSLQEQTDWQNKFSEYEKRKEQKMMEKLEFERQLDLEQREQAQLLLQSNTQKDEILQTVREEQWRLEKGLNKHQQQIQSERQRLQEQLKQMEKGISYRIKNLLEQNRRQKQNSEILKSLEDERIKMEHLMAITQEEADNLRRKEVASAMQQMLAETYK----SKLLQMAYESRRQEMVFQTCSS-----LADMDRKLQQIQHWQQMDQNKAISQILQEEIQKAAFEALQLKKDLMHRQIREQILLIEAELMQLTQLEVKRQEFGNEALQEVIAEQRRALTDMLQQLLKDKKQREEELLA--LEMDSKSEAKQENYWLIQYQRLLNQKPLSLRLQEEGLEQELVDLLKALSAEKYLPIFAHHRFCLEMLEKMSPADLKKIGVEEVGLQHAIIERARE--ILKSARVSPEAQNAGNF---FCGQPPSLFIVLKPGLVATAPLPRNQCC-----HAHVIFLPCGHVCCCTNCGDALRTCPLCRRDIGQRIRI 682
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Match: crebrf (CREB3 regulatory factor [Source:Xenbase;Acc:XB-GENE-988760])

HSP 1 Score: 57.7658 bits (138), Expect = 6.556e-8
Identity = 19/47 (40.43%), Postives = 33/47 (70.21%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C IC+++E  I+F+ CGH+  C+ C+ +L++CP+CR  IK  ++
Sbjct:  553 ERTCKICMDQEVSIVFIPCGHLVVCKDCAPSLRKCPICRGTIKGTVR 599
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Match: Lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:MGI Symbol;Acc:MGI:2684789])

HSP 1 Score: 176.792 bits (447), Expect = 9.762e-46
Identity = 189/693 (27.27%), Postives = 353/693 (50.94%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQL-SNNEDNAVDTVD----------------FSTLDYENFKRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFA---RSLNDKV---IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLL----DTIRQMKIEQNKKICEKEEAIATMNEPNC-----------SLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N LT LP S+GNLL L  L +++N++++LP + L    SL  +D+SEN +   +P  + ++R L   + ++  + +P  ++C  G   + + +CK + +   P +      L  +  +N  D+ D                FS  DYE  KR    +L+ +E E  L+   +       ++   +     T+ +E+ ++   +++     D  R+QL   +K  E   A+ I+ ++  N+  +   ++L  +E+E ++   L  I+++E +NLR++E+ A+M + L    K    + L ++A+    +SL  +    + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L   + E+E + +  Q+++WLIQYQR L+ KP  L   + G++  +  LL  ++A  YLP F  ++  LD L R +  D   VG+    L+  +L    D +   +++   K  E  E +  +  P             + P  + M    +EC +CL  E +++F+ CGH+CCC++C   L+ CPLCR EI + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLVTIKVLDLHENQLTALPDDMGQLTVLQVLNVERNQLTHLPRSIGNLLQLQTLNVKDNKLKELP-DTLGELRSLRTLDISEN-EIQRLPQMLAHVRTLETLSLNALAMVYPPPEVCGAGTAAVQQFLCKESGLDYYPPSQYLLPVLEQDGAENTQDSPDGPASRFSREEAEWQNRFS--DYE--KRKEQKMLEKLEFERRLDLGQREHAELLQQSHSHKDEILQTVKQEQTRLEQDLSERQRCLDAERQQLQEQLKQTEQSIASRIQRLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETENLRQREIAAAMQQMLTESCK----SRLIQMAYESQRQSLAQQACSSMAEMDKRFQQILSWQQMDQNKAISQILQESVMQKAAFEALQVKKDLMHRQIRNQIRLIETELLQLTQLELKRKSLDTETLQEMVSEQRWALSNLLQQLLKEKKQREEELHGILAELEAKSETKQENYWLIQYQRLLNQKPLSLKLQEEGMERRLVALLVELSAEHYLPLFAHHRISLDMLSRMSPGDLAKVGVSEAGLQHEILRRAQDLLAVPRVQPELKPLEN-EVLGALEPPTAPRELQESVRPSAPPAELDMP--TSECVVCLEREAQMVFLTCGHVCCCQQCCQPLRTCPLCRQEISQRLRI 723
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Match: Lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:MGI Symbol;Acc:MGI:2684789])

HSP 1 Score: 176.792 bits (447), Expect = 9.762e-46
Identity = 189/693 (27.27%), Postives = 353/693 (50.94%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQL-SNNEDNAVDTVD----------------FSTLDYENFKRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFA---RSLNDKV---IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLL----DTIRQMKIEQNKKICEKEEAIATMNEPNC-----------SLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N LT LP S+GNLL L  L +++N++++LP + L    SL  +D+SEN +   +P  + ++R L   + ++  + +P  ++C  G   + + +CK + +   P +      L  +  +N  D+ D                FS  DYE  KR    +L+ +E E  L+   +       ++   +     T+ +E+ ++   +++     D  R+QL   +K  E   A+ I+ ++  N+  +   ++L  +E+E ++   L  I+++E +NLR++E+ A+M + L    K    + L ++A+    +SL  +    + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L   + E+E + +  Q+++WLIQYQR L+ KP  L   + G++  +  LL  ++A  YLP F  ++  LD L R +  D   VG+    L+  +L    D +   +++   K  E  E +  +  P             + P  + M    +EC +CL  E +++F+ CGH+CCC++C   L+ CPLCR EI + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLVTIKVLDLHENQLTALPDDMGQLTVLQVLNVERNQLTHLPRSIGNLLQLQTLNVKDNKLKELP-DTLGELRSLRTLDISEN-EIQRLPQMLAHVRTLETLSLNALAMVYPPPEVCGAGTAAVQQFLCKESGLDYYPPSQYLLPVLEQDGAENTQDSPDGPASRFSREEAEWQNRFS--DYE--KRKEQKMLEKLEFERRLDLGQREHAELLQQSHSHKDEILQTVKQEQTRLEQDLSERQRCLDAERQQLQEQLKQTEQSIASRIQRLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETENLRQREIAAAMQQMLTESCK----SRLIQMAYESQRQSLAQQACSSMAEMDKRFQQILSWQQMDQNKAISQILQESVMQKAAFEALQVKKDLMHRQIRNQIRLIETELLQLTQLELKRKSLDTETLQEMVSEQRWALSNLLQQLLKEKKQREEELHGILAELEAKSETKQENYWLIQYQRLLNQKPLSLKLQEEGMERRLVALLVELSAEHYLPLFAHHRISLDMLSRMSPGDLAKVGVSEAGLQHEILRRAQDLLAVPRVQPELKPLEN-EVLGALEPPTAPRELQESVRPSAPPAELDMP--TSECVVCLEREAQMVFLTCGHVCCCQQCCQPLRTCPLCRQEISQRLRI 723
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Match: Birc2 (baculoviral IAP repeat-containing 2 [Source:MGI Symbol;Acc:MGI:1197009])

HSP 1 Score: 56.6102 bits (135), Expect = 1.040e-7
Identity = 18/47 (38.30%), Postives = 33/47 (70.21%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C++ E  I+F+ CGH+  C++C+ +L++CP+CR  IK  ++
Sbjct:  562 ERTCKVCMDREVSIVFIPCGHLVVCQECAPSLRKCPICRGTIKGTVR 608
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Match: Birc2 (baculoviral IAP repeat-containing 2 [Source:MGI Symbol;Acc:MGI:1197009])

HSP 1 Score: 56.6102 bits (135), Expect = 1.040e-7
Identity = 18/47 (38.30%), Postives = 33/47 (70.21%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C++ E  I+F+ CGH+  C++C+ +L++CP+CR  IK  ++
Sbjct:  562 ERTCKVCMDREVSIVFIPCGHLVVCQECAPSLRKCPICRGTIKGTVR 608
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Match: Birc3 (baculoviral IAP repeat-containing 3 [Source:MGI Symbol;Acc:MGI:1197007])

HSP 1 Score: 54.6842 bits (130), Expect = 4.833e-7
Identity = 17/44 (38.64%), Postives = 31/44 (70.45%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            C +C++ E  I+F+ CGH+  C+ C+ +L++CP+CR  IK  ++
Sbjct:  555 CKVCMDREVSIVFIPCGHLVVCKDCAPSLRKCPICRGTIKGTVR 598
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Match: sp|Q80ZI6|LRSM1_MOUSE (E3 ubiquitin-protein ligase LRSAM1 OS=Mus musculus OX=10090 GN=Lrsam1 PE=1 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 6.829e-45
Identity = 189/693 (27.27%), Postives = 353/693 (50.94%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQL-SNNEDNAVDTVD----------------FSTLDYENFKRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFA---RSLNDKV---IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLL----DTIRQMKIEQNKKICEKEEAIATMNEPNC-----------SLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N LT LP S+GNLL L  L +++N++++LP + L    SL  +D+SEN +   +P  + ++R L   + ++  + +P  ++C  G   + + +CK + +   P +      L  +  +N  D+ D                FS  DYE  KR    +L+ +E E  L+   +       ++   +     T+ +E+ ++   +++     D  R+QL   +K  E   A+ I+ ++  N+  +   ++L  +E+E ++   L  I+++E +NLR++E+ A+M + L    K    + L ++A+    +SL  +    + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L   + E+E + +  Q+++WLIQYQR L+ KP  L   + G++  +  LL  ++A  YLP F  ++  LD L R +  D   VG+    L+  +L    D +   +++   K  E  E +  +  P             + P  + M    +EC +CL  E +++F+ CGH+CCC++C   L+ CPLCR EI + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLVTIKVLDLHENQLTALPDDMGQLTVLQVLNVERNQLTHLPRSIGNLLQLQTLNVKDNKLKELP-DTLGELRSLRTLDISEN-EIQRLPQMLAHVRTLETLSLNALAMVYPPPEVCGAGTAAVQQFLCKESGLDYYPPSQYLLPVLEQDGAENTQDSPDGPASRFSREEAEWQNRFS--DYE--KRKEQKMLEKLEFERRLDLGQREHAELLQQSHSHKDEILQTVKQEQTRLEQDLSERQRCLDAERQQLQEQLKQTEQSIASRIQRLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETENLRQREIAAAMQQMLTESCK----SRLIQMAYESQRQSLAQQACSSMAEMDKRFQQILSWQQMDQNKAISQILQESVMQKAAFEALQVKKDLMHRQIRNQIRLIETELLQLTQLELKRKSLDTETLQEMVSEQRWALSNLLQQLLKEKKQREEELHGILAELEAKSETKQENYWLIQYQRLLNQKPLSLKLQEEGMERRLVALLVELSAEHYLPLFAHHRISLDMLSRMSPGDLAKVGVSEAGLQHEILRRAQDLLAVPRVQPELKPLEN-EVLGALEPPTAPRELQESVRPSAPPAELDMP--TSECVVCLEREAQMVFLTCGHVCCCQQCCQPLRTCPLCRQEISQRLRI 723
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Match: sp|Q6UWE0|LRSM1_HUMAN (E3 ubiquitin-protein ligase LRSAM1 OS=Homo sapiens OX=9606 GN=LRSAM1 PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 9.430e-42
Identity = 181/683 (26.50%), Postives = 357/683 (52.27%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNE-DNAVDTVDFST-------LDYENF-----KRNRLDLLKSIESE--LESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV------IVEVNNERRKKLSEMKL-------ELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLR-------QNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMI--MTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P G F+ CKVL+KK L ++ N +  L     +L  L  +  L L  N ++ LPD++ ++  LQVLNV  N L  LP S+GNL  L  L +++N++++LP + +    SL  +++S N +   +P  + ++R L   +  ++ + +P +++C  G   IL+ +CK + +   P +      L  +  +N+ D+ D  T       L+++N      KR    +L+ +E E  LE   +       ++  ++     T+ EE++++   +++     +  R++L   +K  E + ++ I++++  N+  +   ++L  +E+E ++   L  I+++E ++LR+++V ++M + L    K    N L ++A+     + V      + E++   ++ LS  ++       ++  +   QK AF+ LQ++KD+   ++   I  +E+EL+ L+Q E K++    +    +++E+R  L  +LQ L+++K  RE  L + + E+E + +  Q+++WLIQYQR L+ KP  L   + G++ ++  LL  ++A  YLP F  ++  LD L + +  D   VG+    L+       Q LLD  R ++ E    + E     A    P    P      +  + +EC +CL  E +++F++CGH+CCC++C   L+ CPLCR +I + +++
Sbjct:   44 IPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELP-DTVGELRSLRTLNISGN-EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCK----NRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAAR-IQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRI 719
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Match: sp|Q94HV7|SPL1P_ARATH (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.555e-8
Identity = 24/61 (39.34%), Postives = 39/61 (63.93%), Query Frame = 2 
Query: 2204 PQTMIMTHRETE----CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            P T   T R+ +    C +CL+++    F++CGH+CCC  CS  L+ CPLCR  I++++K+
Sbjct:  275 PVTGGGTSRDGDTPDLCVVCLDQKYNTAFVECGHMCCCTPCSLQLRTCPLCRERIQQVLKI 335
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Match: sp|Q8L7N4|SP1_ARATH (E3 ubiquitin-protein ligase SP1 OS=Arabidopsis thaliana OX=3702 GN=SP1 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.115e-8
Identity = 22/44 (50.00%), Postives = 29/44 (65.91%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            C ICL +E   +F+ CGH+CCC  CS++L  CPLCR  I   +K
Sbjct:  296 CVICLEQEYNAVFVPCGHMCCCTACSSHLTSCPLCRRRIDLAVK 339
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Match: sp|Q557E7|CBLA_DICDI (E3 ubiquitin-protein ligase cblA OS=Dictyostelium discoideum OX=44689 GN=cblA-1 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.396e-7
Identity = 19/46 (41.30%), Postives = 32/46 (69.57%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKLM 2377
            C +C++ E   +F++CGH+ CC  CS  LK+CP+CR+ I ++I + 
Sbjct:  618 CTVCMDNEINTVFLECGHLSCCSLCSVKLKKCPICRSRITRVINIF 663
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Match: A0A3M6UJZ9 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00010713 PE=4 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 3.593e-68
Identity = 198/673 (29.42%), Postives = 354/673 (52.60%), Query Frame = 2 
Query:  437 SCINVPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDN-------AVDTVDFSTLD-YENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQ----RKIDKQNELKEIAFARSLNDKV-----IVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAI-----ATMNEPNCSLPQTMIMTHRE--TECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
                VP+G +SLC+VL +K+  L  +        G +L+DL+ L  L L  N I+ LP  ID ++ LQVLNV  N L  +P S+GNL  L  L++Q N +  LP   + +  SL  +++SEN     +P  +  +R L       N + FP +D+  +G   I+K +CK  +I   P +    N L N   +        VD +  STL  +E  K  R   +  IE +L      Q+  A  A  +R    + +   EA+I +       ++++ R++L++ + A E    + +  I+ ++   Q  E +LDE+E E M+   L +++++E + LRK+E+L SM+  L  Q    R I +    ++   ++++N+ +     ++ + NE+ +    +  E+  +E  Q  A Q+LQ+Q D +  R+T  I  ++ EL  L+  E +K++E+ D +   L  RR+ L  ++  L+ ++  RE  L KR++EME +R+ D +D+WLIQYQR L+ KP  L      D  + ++L+   A +Y   F R++   + L+  T+ + K +G++   +R+ +++ + Q ++  + K  EKE  I          P+ + P   I+ HR+  TEC IC+++ + ++ ++CGH+CCC  CS+++  CP+CRN + + I++
Sbjct:   39 GATEVPKGVYSLCRVL-QKEALLLFDNDLSNLKGGGDLKDLSNLRVLDLHDNHITALPAAIDVLKSLQVLNVQGNKLKAVPPSIGNLASLQSLILQANDLRGLP-PEIGNLKSLRTLNISENSNLPGVPPTLARVRTLENIILDVNIVSFPPRDVAAEGTASIMKYLCKVAEIEYVPPSKHLLNVLGNGTASNKQLNPTPVDDLVASTLSKHEAEKERRRQQMIEIEKQLHETELEQQALAAAASKQRTDLMDKIRMAEAEIDDLTLMQQQQQEVERKKLVSAMAADEQLTNDTVAMILQLDSRAQKAEMILDEMEKERMRTEQLVKVTQEEAEKLRKEEMLVSMARLLESQESQSRLIREYERARDRTASQAMNEALEEDVRLLGILNEQYEDRDTLISEISKEERAQMEAIQVLQLQTDAKHRRITGQISMLQDELTKLTLTELEKKDERQDAERAALESRREALTGLMVQLVSEQQKREDELMKRLVEMEHKRETDIEDYWLIQYQRLLESKPDTLLAKQ-CDGVISDILAEAEAQDYASHFARHRITWEMLKTMTDQELKELGIHELGVRKAIMNVV-QERLRFDSKAKEKESKIENPEVPVPQPPSSNTPVAPIVEHRDMTTECVICMDQRSDVVLLNCGHVCCCFNCSSSITSCPMCRNPVVQRIRI 707
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Match: A0A0L8IDQ0 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22014520mg PE=4 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 6.965e-68
Identity = 212/688 (30.81%), Postives = 357/688 (51.89%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQ---IPINMSFFNQLSNNE----------DNAVDTVDFSTL------DYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFH-------QRKIDKQNE-LKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSG-LDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKK-------ICEKEEA----IATMNEP-----NCSLPQTMIMTHRE--TECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            +P   FSLCKVL K+ L L++N +  L +    + DL  +  L    N I  LP+NID+++ LQVLN+  N L  +P S+GNL +L  L ++ N+++++P +L   +S    +D+S N +   +P  +  LR L      +  +  P  ++C QG   I+K +C  +    IP +      L ++E          D++V  +    L       Y N +  +     +   + E   K     A  AL E+    +T+A+E+ K+   + ++  +++L R+ L+  ++  E     L+ ++M +N   + QE+L++ IE E  ++   Y +  +EI NLR++EVL  M E L         +RK D + +     A  + +N  V+ ++   +  +  EM   L  QE  QK AF+ L +QKD R  R++  I  +E EL  L+  E +++E ++D + NIL ++R  L  +L  LI ++  RE  L  R+ EME+QR QDQ D+WL+QYQR +D KP  L +    L++ V ++L +  A +Y+P F R++   + L   TEDD K +G++   +R+ ++  +R  + +  KK       +C  E       A   EP     N +     I+T R   TEC ICL+  T I+F++CGH+CCC +CS +L+ CPLCR+ I + I++
Sbjct:   87 IPSNVFSLCKVLNKEILLLHDNWLTSLKAGDCGMSDLVKIRVLDAHNNSIKHLPENIDQVRSLQVLNLEGNKLKKIPESIGNLKFLQSLNLKGNKLKEIPTSLSNLSSL-RTLDLSNN-QLTNLPTELWKLRALENLLVDAANMVHPPAEVCSQGTEAIMKFLCSESGEEYIPPSQFLLQVLDSSEKKHQMSSTFSDHSVKLLQEDALMMQDIEKYANLQEKKRQERMAFNRQYEEDEKKFAKMAASALKEKDHLVSTIAQEQIKLNEDLAELSNKKELDRQNLVTCLEDIEERTNRLLNDLMQMNEKARKQEELMEAIEKEKDEQDQYYIVRYEEIANLRRKEVLNRMEEILNSSAYLETCRRKHDAEKKHAARNALEKEMNSTVLNDILQSKSAEQDEMVKVLTQQEELQKAAFEALLMQKDSRHSRVSSQIALIEKELAQLTAIEIERKELRIDMEKNILADKRIALTGMLMQLIAERDKREEQLKMRLTEMEEQRLQDQTDYWLVQYQRLMDRKPQALIDQEWLLEIPVLKILEQTGAQDYIPLFARHRITSETLCHMTEDDLKQMGVHELGVRRAIM--VRLQEYQSTKKVDMLPPDVCTGEAVRPLPTAPAPEPSPSEENPTSSTQPIVTARGLYTECSICLDRTTNIIFLNCGHVCCCTECSVDLEFCPLCRSNILQKIQV 770
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Match: A0A210QV31 (E3 ubiquitin-protein ligase LRSAM1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04050 PE=4 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 7.324e-68
Identity = 178/649 (27.43%), Postives = 331/649 (51.00%), Query Frame = 2 
Query:  596 ISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI----PINMSFFNQLSNNEDNAVDTVDFS-------------TLDYENFKRNRLDLLK-SIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNEL----KEIAFARSLNDKVIVEVNNER----RKKLSEMKLE--LENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWN-DSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLL-------------------------DTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHR-ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKLM 2377
            + +LPD++ K+++LQ LNV  N     P S+  +  L                         ++D+S+N     +P  +  +  L      +  + +P  D+C QG   I++ +C  +++    P N      L + +D++   V+ S             TL+ +  +  +  L K  +E  L    + Q   A  A + +  F + +AE++ KI  ++ ++ +++D  R +L+ D+K  E    NLI++++ +N   +  E+LLD++E+E ++K  L+ +  +E+QNLRK+EVL +M   L      +K  +     KE    ++L D+ ++           +++  M +   L+ Q   Q+ AF+ LQ+QKD +  R++  I  ++ EL+ L+  E +KR  + + + N++ E+R  L+E+L  L+ ++  R+I+L +R+ EME+QRQ  Q D+WL+QYQR +D KP  L + +  L++ V  +L   +A +Y+P F R++  ++ L + +EDD K +G++   LR+++L                         D+  + K++ N+   E     A   EP C+  Q+ I+     TEC +CL++ T ++F++CGH+C C  CS  ++ECPLCR  I + +KLM
Sbjct:  160 LGRLPDSLTKLRHLQTLNVKGNKFKIFPMSICRMKSLR------------------------LLDISDN-LITSLPKELCQVVTLETIILDAEKMTYPPADVCCQGTEAIMQFLCDESRVEYLAPSNF-LLRVLDSPDDSSPSGVNDSLRQTAAEEKILLKTLEDQRTQTEKKRLEKIELERNLAENQREQAYLASLAQNRKQSFIDMVAEDQNKIERELEELSMKKDEERSRLLTDLKMIEEGTNNLIQKMLEMNEKARKNEELLDQLENERIEKEELFVVRWEEVQNLRKKEVLDAMKYILTETEGFEKLRQDYTTNKEFTMQKALEDEAMLASGQVESILYHREVGNMVMLDILKEQGQLQREAFEALQLQKDTKHQRISTQIALIQEELMNLTAVEIEKRSLRTEEEINVVAEKRIALIELLSQLMCEQEKRQILLKRRLKEMEQQRQDGQTDYWLVQYQRLMDKKPQALIDQERQLEIAVVTILEDSSADDYIPLFARHRITIETLLQLSEDDLKQMGVHEMGLRKSILRNLDHYREKKGQQYPKPKEENKGAWDSYLESKVQDNEGESEATLPSAPPVEP-CTTRQSSILARGINTECVVCLDKSTDVIFLNCGHVCTCIDCSVKVQECPLCREPIAQRVKLM 781
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Match: A0A564YV18 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS9836 PE=4 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 2.411e-64
Identity = 185/622 (29.74%), Postives = 317/622 (50.96%), Query Frame = 2 
Query:  602 KLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTLDYENFKRNR-LDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQ--EVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV---IVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTI-RQMKIEQN-----------KKICEKE------EAIATMNEPNC----SLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            + P  I  +  LQVL++S+N +  +P+ +  L  L +  +++NQI  LP        SLE +D+  NP    +P+ +  L  L++ +  S  L FP QDIC+ G++ I+  + KA +I    + ++   ++S  +D    +       + +F  NR  DL + +E EL     +    A +    R+     LA+EE  + N++  +  +RD  R++L+N ++     A NLI+ +       + Q+  +++I+  ++     + +S +  Q + +   ++L +    +   R I K      IA   S  D V   I  V N R+   +++  +   +E EQ  AF+ LQ + D+R  ++   I  +E+EL  L+QAEQ+++  +        +ERR+ LV +L  L +QK LRE  L KR++EME+Q+ +DQ D+WL+QYQR +D KP  L  +S L  E++E+L    A  YLP F  ++   DQ    T++D   +G+    +R+++L+ I    KI+++             I EKE      E I   + P      S P   I+ H E ECCIC N +  I+F+ CGH+CCC  CS  + +CPLCR  +++ ++L
Sbjct:   90 QFPPEIFSMTLLQVLDLSHNEIKTVPDQICQLRSLHKFNLRDNQIHSLP-TKFGDLRSLEDLDLDGNP-LSCLPVEMGRLINLKQLSLPSTSLTFPPQDICKAGVLAIILYLRKAGKILDDELELAE-TKISTGQDYDTSSSAIENQGFCDFGLNRRSDLHRKVEEEL----NAHAELAKRVAKTRVSSVKKLAQEEHVMQNELLFLQKKRDEKRKELVNSLRGTLDHADNLIKLLQ------KRQQITVEDIDLPSLDSLPYFTLSDENRQKILESMNDILVASETAMMRHRDIKKN-----IAMTNSNPDDVNPAIQSVLNSRQANQAKLAAQFAIEEREQMKAFECLQQEHDLRRRQIIGDIRLIEAELCRLTQAEQERKATQAAASTTDFSERRKDLVRLLAELRKQKKLRESELHKRLVEMEEQKARDQSDYWLVQYQRLIDRKPAVLNEESFLSDEIREVLVTAGAENYLPNFEYHRITPDQFSALTDEDLIRIGVSAVGVRKSILEAIVAHGKIQKDWMVEPSAPSKSSVIVEKESDEIKGEIINESSSPEAGTKPSAPPADIVAHFEAECCICQNAQCSIIFLPCGHVCCCVTCSAKVSQCPLCRVIVQQRVQL 693
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Match: A0A0R3SW04 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS9802 PE=4 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 2.155e-63
Identity = 167/563 (29.66%), Postives = 286/563 (50.80%), Query Frame = 2 
Query:  779 EVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTLDYENFKRNR-LDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQ--EVLASMSENLFHQRKIDKQNELKEIAFARSLNDKV---IVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTI-RQMKIEQN-----------KKICEKE------EAIATMNEPNC----SLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            E +D+  NP    +P+ +  L  L++ +  S  L FP QDIC+ G++ I+  + KA +I    + ++   ++S  +D    +       + +F  NR  DL + +E EL     +    A +    R+     LA+EE  + N++  +  +RD  R++L+N ++     A NLI+ +       + Q+  +++I+  ++     + +S +  Q + +   ++L +    +   R I K      IA   S  D V   I  V N R+   +++  +   +E EQ  AF+ LQ + D+R  ++   I  +E+EL  L+QAEQ+++  +        +ERR+ LV +L  L +QK LRE  L KR++EME+Q+ +DQ D+WL+QYQR +D KP  L  +S L  E++E+L    A  YLP F  ++   DQ    T++D   +G+    +R+++L+ I    KI+++             I EKE      E I   + P      S P   I+ H E ECCIC N +  I+F+ CGH+CCC  CS  + +CPLCR  +++ ++L
Sbjct:   52 EDLDLDGNP-LSCLPVEMGRLINLKQLSLPSTSLTFPPQDICKAGVLAIILYLRKAGKILDDELELAE-TKISTGQDYDTSSSAIENQGFCDFGLNRRSDLHRKVEEEL----NAHAELAKRVAKTRVSSVKKLAQEEHVMQNELLFLQKKRDEKRKELVNSLRGTLDHADNLIKLLQ------KRQQITVEDIDLPSLDSLPYFTLSDENRQKILESMNDILVASETAMMRHRDIKKN-----IAMTNSNPDDVNPAIQSVLNSRQANQAKLAAQFAIEEREQMKAFECLQQEHDLRRRQIIGDIRLIEAELCRLTQAEQERKATQAAASTTDFSERRKDLVRLLAELRKQKKLRESELHKRLVEMEEQKARDQSDYWLVQYQRLIDRKPAVLNEESFLSDEIREVLVTAGAENYLPNFEYHRITPDQFSALTDEDLIRIGVSAVGVRKSILEAIVAHGKIQKDWMVEPSAPSKSSVIVEKESDEIKGEIINESSSPEAGTKPSAPPADIVAHFEAECCICQNAQCSIIFLPCGHVCCCVTCSAKVSQCPLCRVIVQQRVQL 597
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:103025730])

HSP 1 Score: 211.075 bits (536), Expect = 2.128e-57
Identity = 189/682 (27.71%), Postives = 344/682 (50.44%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNED-NAVDTVDFSTL-------DYENFKRNRLDLLKSIESELESVMKSQKTFA--DKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASM----SEN-----LFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSL---------PQTMIM--------THRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            VP   FS+CKVL+KK   L+ N+++ L   G ++  L  +  L L  N ++ LPD+I ++  LQVLN  +N +  LP S+GNL  L  L ++ N + ++P + +   SSL  +D+SEN     +P  + N+R L      ++ + +P   +C  G  EI + +C    +   P +      L N+    + D VD   +       DYE  K  +     + E +LE   + Q      + +  E M+    L +E  ++   + +    ++  R++++  V+ AE   +  I  ++  N+      ++L  +E E ++  HL  I+++E  +LRK++V  +M    SE+     L    +  +Q+ + E   +    DK   +V + ++   S+   ++  +E  QK AF+ LQ+Q+D     +   I  +E+EL+ L++ E K++    +    +L E+R+ L ++LQ L++QK  RE+ L   ++EME +    Q ++W+IQYQR LD KP  L   ++G+D ++  LL +++A  YLP    ++   + L   T  D   +G+    ++++LL   R+      KK+ E+ EA A +  P   L         P+T +             +EC +C+  E++++++ CGH+CCC+ CS+ L  CPLCR++I + I+L
Sbjct:   92 VPSSAFSICKVLQKKVFILHGNELRSLLPKGCSISSLETIKVLDLHENKLTSLPDDIGQLSALQVLNAEHNQIKTLPESIGNLHNLQTLNVKGNCLSQIP-SSVGKMSSLRTLDLSEN-SIRELPKELANVRTLESLTLDASTMNYPPVSVCTAGTEEIQRYLCSELGVEYCPPSQYLLPVLENDSSKQSNDCVDGEVMAWQSKFMDYEKRKEQKQLEKLNFEKDLEEKQREQAQLVVLNNSRKEGMLLSVKLEQE--RVEKDVCQQQKTQEAERQRVLEKVRQAETCISTRISNLLLDNKRQAKSAEILQAMEEERIRVEHLTAITQEEANSLRKKDVAVAMQTMLSESYSMRLLQEASEARRQSMMSEACKSMETMDKKFEQVLSLQQLDKSKAICQILQEEEMQKAAFEALQLQRDSVHGYIRNQIKLIEAELMQLTKLEVKRKNLDAENLQEVLAEQREALSDMLQQLLKQKDQREVELKHILMEMELKSDSTQQNYWMIQYQRLLDAKPLSLRMQEAGVDKDLVSLLCKLSAQHYLPILAHHRITAEALRHMTTRDLSKLGINEVGVQKSLLHWAREQPCS-PKKLHEEREAGAVL--PTAPLQEQLTPPLTPKTPLTPTVPSPMDGPSSSECVVCMEAESQVIYLPCGHVCCCQVCSDALYSCPLCRSDISQRIRL 766
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Match: birc2 (baculoviral IAP repeat containing 2 [Source:NCBI gene;Acc:103025481])

HSP 1 Score: 54.6842 bits (130), Expect = 3.890e-7
Identity = 17/47 (36.17%), Postives = 34/47 (72.34%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C+++E  I+F+ CGH+  C++C+ +L++CP+CR  +K  ++
Sbjct:  581 ERTCKVCMDKEVNIVFIPCGHLVVCKECAPSLRKCPICRGLVKGTVR 627
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Match: birc2 (baculoviral IAP repeat containing 2 [Source:NCBI gene;Acc:103025481])

HSP 1 Score: 53.9138 bits (128), Expect = 5.237e-7
Identity = 17/47 (36.17%), Postives = 34/47 (72.34%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C+++E  I+F+ CGH+  C++C+ +L++CP+CR  +K  ++
Sbjct:  567 ERTCKVCMDKEVNIVFIPCGHLVVCKECAPSLRKCPICRGLVKGTVR 613
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Match: mul1b (mitochondrial E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:103040716])

HSP 1 Score: 51.2174 bits (121), Expect = 2.356e-6
Identity = 19/50 (38.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query: 2234 TECCICLNEETKILFMDCGHICCCEKCSNNL---KECPLCRNEIKKIIKL 2374
            + C +CL  +   +F++CGH+C CE C   L   ++CP+CR  I +I+ L
Sbjct:  300 STCTVCLARDRSCVFLECGHVCSCEPCYRALPQPRKCPICRANIDRIVPL 349
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Match: mylipa (myosin regulatory light chain interacting protein [Source:NCBI gene;Acc:103027301])

HSP 1 Score: 50.447 bits (119), Expect = 5.330e-6
Identity = 18/42 (42.86%), Postives = 27/42 (64.29%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKI 2365
            C +C  EE    F  CGH+ CC+ C+  L+ CP+CR+E++ I
Sbjct:  384 CMLCCEEEIDAAFCPCGHMVCCQNCAKQLQSCPVCRSEVEHI 425
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Sea Lamprey
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:ZFIN;Acc:ZDB-GENE-060526-97])

HSP 1 Score: 140.969 bits (354), Expect = 3.682e-35
Identity = 134/529 (25.33%), Postives = 256/529 (48.39%), Query Frame = 2 
Query:  596 ISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKANQI---PINMSFFNQLSNNEDNAVDTVDFSTLDY--------ENFKR--NRLDLLKSIESELESVMKSQKT----FADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVL--ASMSENLFHQRKIDKQNELKEIAFARSLNDKV--------------------IVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDD 2062
            +  LP+ + ++  LQVLNV  NNL  LP S+  L  L  L +++N+I +LP +L    S    +D+S N +   +P+++ ++R L         + FP QD+ +QGI  + + +C+ + +   P +    + L+ +     D  D + L +        + FK   N+ ++ +  + E E  +  Q        + A  +R    +++ E  A++  +++     +D  R ++  ++   E  A +L+  I++       ++ L + ++ E ++   L  I+++E   LR+ +V   A  S +L        Q+   ++  A  L+ +                     +V   ++R+K+     L+   +E  QK AF+MLQ+Q+D    R+   I  +E EL+ +S  E K++ +  +    +L E RQ L ++L  L+++K  RE+ L   + EMEK+R++D  D+WL+QYQR +  KP  +   +S LD+ +KELL+   +   +P F R++  L  L   T +D
Sbjct:   91 LKALPEEMWQLSALQVLNVEGNNLMALPESVVRLRQLQTLNVRDNKISELPKDLDGLQSLRN-LDISLN-QISKLPLSLAHIRTLETLQLDVGRISFPPQDVVRQGIEAVKRFLCEESGLEYSPPSQHLLSVLTASPQRCPDVTDNAELQHSLQQAAWQDKFKEFENKKEIRRKEQLEFERALGQQHMDMALLVNDATLQRDELLHSVKEGLAELEREVDGQRRRQDATRVRMTEELLRMEKKAGDLVSHILSKRDWFTQKKDLQEAMDRERLEMELLVCITQEETSALRRNDVAGEAVTSPHLV-------QSNALQLTAAGCLSGRAKAASSACSSILTEEEEKLQCVVSGRHDRQKQAIASILQ---EELIQKEAFEMLQLQRDAWHSRIRHQIGLIEQELLHISMLEMKRKGQNQEQMQGVLVEERQALGDLLHQLLKEKGARELQLQDLLEEMEKKREEDSMDYWLVQYQRLMHSKPQPMRLQESLLDVGLKELLAETGSPGLIPLFARHRVSLAALAAMTHED 607
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Sea Lamprey
Match: mul1a (mitochondrial E3 ubiquitin protein ligase 1a [Source:NCBI gene;Acc:553180])

HSP 1 Score: 52.373 bits (124), Expect = 2.278e-7
Identity = 27/102 (26.47%), Postives = 48/102 (47.06%), Query Frame = 2 
Query: 2078 MYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKE---CPLCRNEIKKIIKL 2374
            +Y R   +   D +R+ +  +  ++  ++ A  T  E +  LP           C +CL+     + +DCGH+CCC  C   L     CP+CR+ + +I+ L
Sbjct:  263 IYRRAREERRWDKLRREQEAEVARLAPEDGAGGT--EGDGRLP-----------CVVCLSAPRGCVLLDCGHVCCCLPCYRVLPRPVCCPVCRSPVSRIVPL 351
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Sea Lamprey
Match: birc2 (baculoviral IAP repeat containing 2 [Source:ZFIN;Acc:ZDB-GENE-030825-6])

HSP 1 Score: 50.8322 bits (120), Expect = 9.311e-7
Identity = 14/44 (31.82%), Postives = 32/44 (72.73%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            C +C+++E  ++F+ CGH+  C  C+++L++CP+CR  ++  ++
Sbjct:  529 CKVCMDKEVSVVFIPCGHLVVCRDCASSLRKCPICRAVVRGTVR 572
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Match: EDO43423 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYU6])

HSP 1 Score: 208.379 bits (529), Expect = 4.829e-57
Identity = 198/757 (26.16%), Postives = 360/757 (47.56%), Query Frame = 2 
Query:  344 HYARIIDNTIQRFGGDKIDTLLDFKHETF-LKSC--INVPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDIC---QQGIVEILKSV------CKANQIPINMSFFNQLSNNEDNAVDTVDFSTLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVL-----------------------------------------------ASMSENLFHQRKIDKQNELKEIAFARSLNDKVI--VEVNNERRKKLSE-------MKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQ------------TMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            H  ++  +  QR    ++    +    TF L  C    +P+  FSLC+VLRK+   L N+        G  L+DL     L L  N ++ LP ++D+++ LQVL+V+NN L  LP ++G L  L  L +Q N ++ LPL +         ++VS NPK   +P ++   R L +     + +  P +      +    +ILKS+      C ++  PI+    + L+++      +VD      ++ K+ ++ +L+    EL+S    Q+  A++A          + + EA + + I     +++  R+ + + + A E   ++ +  I+ IN     +E LLD++E E ++  HL ++++DE + LRK+EV+                                               ASM+  L ++R  ++     E    +++ D +   VE N++ +  LSE       +K ++  QE+ Q  A Q LQ+QKD +  RLT+ I  ++ EL  L+  E +KR+E+ + +   L  +R+ +  ++  L+ Q+  RE  L KR++EME++R  D +D+WLIQYQR LD KP  L  +   D  + ++L    A +Y   F R++     L+  T ++ K +G++ R +R+ ++  +    + + K    KE+ I+   +P  S PQ            TM       EC ICL+  + ++ + CGH+CCC  C+  +  CP+CR  + + +++
Sbjct:    5 HTRKVSRDETQRRLEQRLVVAKESPESTFDLSDCGITELPKDVFSLCRVLRKQ-TVLLNDNDLPNLKGGGQLKDLADARILVLSNNRLTSLPADLDELRSLQVLDVANNKLKSLPKAIGGLSSLQTLDVQGNNLQSLPLEIGNLKLLRS-LNVSNNPKLDALPASLAYCRLLEEITLDMDKISVPPKGAGIEYEPSSKQILKSLDPACDSCHSSVDPIDKLVADALNSH------SVD------QDKKQEQMWMLEKQMRELDS---EQQALANQANRSHDDLLQRIQQAEADMDSDILWEQYKQESRRDNMSSLMAADEQLNSDTVAMILQINERSGKREALLDQLEIEKLRTDHLVKVTQDEEERLRKEEVIGKFLNLVTGPQLASKYWKHMTKKNGSDGQGAQPRPYRAQALRLIPDVASMARLLENRRNQERILNEYESTRQQAMRDAIKSEVEANSQVQDVLSEQDQDRGSLKRQISTQEHAQMQAIQALQLQKDAKHRRLTDQIGMLQDELAYLTAVELEKRDEEQEAEKVALAAKRESITSLMSQLVDQQRQREGELMKRLVEMEERRVTDVEDYWLIQYQRLLDSKPQSLL-EKECDHVIADVLKEAEAEQYCSLFARHRITWKMLKSMTSEELKEMGIHERGIRKGIMAVVEARALVEGKAR-RKEKEISQAEDPERSHPQPSAPPVEATATPTMSNCSLYPECVICLDNRSDVVMLPCGHVCCCSNCAGAVSACPICRQTLSQRVRM 742
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Match: EDO39401 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA58])

HSP 1 Score: 55.4546 bits (132), Expect = 4.724e-8
Identity = 16/50 (32.00%), Postives = 34/50 (68.00%), Query Frame = 2 
Query: 2225 HRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            ++ T+C ICL  +  ++ ++CGH+C C  C+  + +CP+CR +I +++ +
Sbjct:  290 NQGTQCVICLENQRNVVLLNCGHVCSCRTCAQQIHQCPVCRGDIVRMVPI 339
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Match: EDO43695 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXW6])

HSP 1 Score: 48.521 bits (114), Expect = 1.423e-7
Identity = 19/48 (39.58%), Postives = 29/48 (60.42%), Query Frame = 2 
Query: 2213 MIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEI 2356
            M  ++ +  C IC++ E    F  CGH+ CC+ C++NL  CPLC+  I
Sbjct:    1 MDTSYNDLTCQICMDAEVNTAFCPCGHVYCCQTCASNLYYCPLCKTFI 48
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Match: EDO30381 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0Y6])

HSP 1 Score: 53.5286 bits (127), Expect = 1.602e-7
Identity = 24/65 (36.92%), Postives = 37/65 (56.92%), Query Frame = 2 
Query: 2192 NCSLPQT-----MIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            N SLP T     +     E  C IC++ E  I+F+ CGH+ CC  C+  ++ CP+CR  I++ I+
Sbjct:  264 NLSLPSTNEKQKLERMQEERLCKICMDAEVGIVFLPCGHLSCCPGCAEGMELCPMCRAPIRETIR 328
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Match: EDO41568 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3Y5])

HSP 1 Score: 52.7582 bits (125), Expect = 3.812e-7
Identity = 18/46 (39.13%), Postives = 30/46 (65.22%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKLM 2377
            C +C++E+   + + CGH+ CCE+C+ NL+ CP+CR  I  + K  
Sbjct:  377 CKVCMDEQINAVLIPCGHMVCCEQCAMNLEACPVCRGAIDHVQKAF 422
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:101162496])

HSP 1 Score: 176.407 bits (446), Expect = 9.789e-46
Identity = 188/692 (27.17%), Postives = 326/692 (47.11%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKA---NQIPINMSFFNQL----SNNEDNAVDTVDFSTLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENL-------FHQRKIDKQNELKEIAFARSLN------DKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEE---------------------AIATMNEPNCSLPQTMIMTH--------RETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            VP   FS+CK+ +KK L L++N ++ L   G ++  L+ L  L L  N ++ LP++I K+  LQ+LNV  N L  LP S+G+L  L  L ++ N + +LP + + S  SL  +DVS+N     +P  +  +R L  F+  +  + +P   +C +G   I   +C        P +      L    S    + VD  D      E +K  +     + E  L+   +            R    N++ +++ +     N+    ++  R  L+  V+ AE   +  I  ++  N   +   K L ++E E +    L  I++ E  +LR++EV A+M + L         Q   D + +       RSL       DK++     ++ K ++E+  E    E  QK AFQ LQ+Q+D     +   I  +E EL+ L++ E K+R    +    +L E R  L ++LQ L++Q++ RE  L + ++EME + + +Q ++W+IQYQR LD KP  L   ++G++ E+  LL R++A  YLP    ++   + L      D K +G+    +++ LL      +     K   K++                     A+ T + P CS P T +             +EC +C+   +K++F+ CGH+CCC+ CS+ ++ CPLCR+ I + I+L
Sbjct:   44 VPSSAFSICKLFQKKVLILHSNDLKYLLPKGCDISTLSTLKVLDLHENKLATLPEDIGKLVSLQILNVEKNRLKVLPESIGDLHLLQTLNLKGNCLCELP-SSMGSLRSLRTLDVSDN-CITELPRALAYIRTLEIFSLDAAAMSYPPACVCTEGTTSIQSFLCAELGEEYCPPSQYLLPVLETDCSTQNSDCVDDGD------EAWKEKKQQEKLAFERHLQQKQQEHTQLLLMNDSHRKNILNSVRKQQEREELGFNEQQRAQEAERLVLLEKVRQAEDTISGRIGTLLMDNSRQETNAKFLQDMEEERIYMEQLTAITQQEASSLREREVAAAMQKMLSDSCAMSVLQEASDHRRQSLVSEACRSLETLDKKFDKMLSLQVLDKSKAIAEILQE----EEMQKAAFQALQLQRDAVHSYIRSQIKLIEGELMQLTKLEIKRRTLDSENLEEVLVEERAALSDLLQQLMKQRSQREQELQQVLVEMELKSESNQQNYWMIQYQRLLDAKPLSLRMQEAGVERELVHLLCRLSAQHYLPIVAHHRLTAEALRNMKASDLKKLGINETGIQKALLTWALGHQPTGAGKPARKDDSNEAGPSAPPPPSPPVPQSSSAMQTPSPP-CS-PGTPVTPSGPPLLEGPGSSECVVCMETGSKVIFLPCGHVCCCQVCSDAVQNCPLCRSNISQRIRL 721
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Match: lrsam1 (leucine rich repeat and sterile alpha motif containing 1 [Source:NCBI gene;Acc:101162496])

HSP 1 Score: 174.866 bits (442), Expect = 3.222e-45
Identity = 191/698 (27.36%), Postives = 329/698 (47.13%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSNNNLTCLPXXXXXXXXXXXXXXXXXXXEKLPLNXXXXXXXXEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVEILKSVCKA---NQIPINMSFFNQL----SNNEDNAVDTVD------FSTLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENL-------FHQRKIDKQNELKEIAFARSLN------DKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYL-WNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEE---------------------AIATMNEPNCSLPQTMIMTH--------RETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            VP   FS+CK+ +KK L L++N ++ L   G ++  L+ L  L L  N ++ LP++I K+  LQ+LNV  N L  LP S+G+L  L  L ++ N + +LP + + S  SL  +DVS+N     +P  +  +R L  F+  +  + +P   +C +G   I   +C        P +      L    S    + VD  D      FS  +YE  K  +     + E  L+   +            R    N++ +++ +     N+    ++  R  L+  V+ AE   +  I  ++  N   +   K L ++E E +    L  I++ E  +LR++EV A+M + L         Q   D + +       RSL       DK++     ++ K ++E+  E    E  QK AFQ LQ+Q+D     +   I  +E EL+ L++ E K+R    +    +L E R  L ++LQ L++Q++ RE  L + ++EME + + +Q ++W+IQYQR LD KP  L   ++G++ E+  LL R++A  YLP    ++   + L      D K +G+    +++ LL      +     K   K++                     A+ T + P CS P T +             +EC +C+   +K++F+ CGH+CCC+ CS+ ++ CPLCR+ I + I+L
Sbjct:   44 VPSSAFSICKLFQKKVLILHSNDLKYLLPKGCDISTLSTLKVLDLHENKLATLPEDIGKLVSLQILNVEKNRLKVLPESIGDLHLLQTLNLKGNCLCELP-SSMGSLRSLRTLDVSDN-CITELPRALAYIRTLEIFSLDAAAMSYPPACVCTEGTTSIQSFLCAELGEEYCPPSQYLLPVLETDCSTQNSDCVDDGDEAWKNKFS--EYEKRKEKKQQEKLAFERHLQQKQQEHTQLLLMNDSHRKNILNSVRKQQEREELGFNEQQRAQEAERLVLLEKVRQAEDTISGRIGTLLMDNSRQETNAKFLQDMEEERIYMEQLTAITQQEASSLREREVAAAMQKMLSDSCAMSVLQEASDHRRQSLVSEACRSLETLDKKFDKMLSLQVLDKSKAIAEILQE----EEMQKAAFQALQLQRDAVHSYIRSQIKLIEGELMQLTKLEIKRRTLDSENLEEVLVEERAALSDLLQQLMKQRSQREQELQQVLVEMELKSESNQQNYWMIQYQRLLDAKPLSLRMQEAGVERELVHLLCRLSAQHYLPIVAHHRLTAEALRNMKASDLKKLGINETGIQKALLTWALGHQPTGAGKPARKDDSNEAGPSAPPPPSPPVPQSSSAMQTPSPP-CS-PGTPVTPSGPPLLEGPGSSECVVCMETGSKVIFLPCGHVCCCQVCSDAVQNCPLCRSNISQRIRL 731
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Match: birc2 (baculoviral IAP repeat-containing protein 2 [Source:NCBI gene;Acc:101172600])

HSP 1 Score: 55.4546 bits (132), Expect = 2.297e-7
Identity = 17/47 (36.17%), Postives = 34/47 (72.34%), Query Frame = 2 
Query: 2231 ETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIK 2371
            E  C +C+++E  I+F+ CGH+  C++C+ +L++CP+CR  +K  ++
Sbjct:  657 ERTCKVCMDKEVNIVFIPCGHLVVCKECAPSLRKCPICRGLVKGTVR 703
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Match: mul1b (mitochondrial E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101160482])

HSP 1 Score: 54.6842 bits (130), Expect = 2.440e-7
Identity = 19/48 (39.58%), Postives = 32/48 (66.67%), Query Frame = 2 
Query: 2240 CCICLNEETKILFMDCGHICCCEKCSNNL---KECPLCRNEIKKIIKL 2374
            C +CL+ E   +F++CGH+C C +C   L   K+CP+CR  I++++ L
Sbjct:  332 CTVCLSRERSCVFLECGHVCACAQCYEGLTEPKKCPICRAPIERVVPL 379
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Match: xiap (E3 ubiquitin-protein ligase XIAP [Source:NCBI gene;Acc:101166883])

HSP 1 Score: 50.8322 bits (120), Expect = 2.577e-6
Identity = 22/59 (37.29%), Postives = 34/59 (57.63%), Query Frame = 2 
Query: 2201 LPQTMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKLM 2377
             P+ +    +E  C ICL+E   I+F+ CGH+  C+ CSN L +CP+C   I + I+  
Sbjct:  240 FPKELEKLRQEKRCKICLDENACIVFIPCGHLASCKACSNKLNQCPICCAAIAQKIRTF 298
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Match: SMESG000046440.1 (SMESG000046440.1)

HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0
Identity = 420/438 (95.89%), Postives = 426/438 (97.26%), Query Frame = 2 
Query:  974 FNQLSNNEDNAVDTVDF-STLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEE-TKILFM 2281
            +N +S   DN +D + +  TLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEE T  LF+
Sbjct:   80 YNLISKLPDN-IDKIQYLQTLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEEVTYFLFL 516

HSP 2 Score: 58.9214 bits (141), Expect = 1.067e-8
Identity = 66/70 (94.29%), Postives = 67/70 (95.71%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSN 658
            VPQGCFSLCKVLRKKKLFLNNNQIQLLNSNGNNLEDLTLLTDLHLEYNLISKLPDNIDKIQYLQ L+  N
Sbjct:   34 VPQGCFSLCKVLRKKKLFLNNNQIQLLNSNGNNLEDLTLLTDLHLEYNLISKLPDNIDKIQYLQTLDYEN 103
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Match: SMESG000046440.1 (SMESG000046440.1)

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 354/368 (96.20%), Postives = 359/368 (97.55%), Query Frame = 2 
Query:  974 FNQLSNNEDNAVDTVDF-STLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNV 2074
            +N +S   DN +D + +  TLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNV
Sbjct:   80 YNLISKLPDN-IDKIQYLQTLDYENFKRNRLDLLKSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLERDLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNV 446

HSP 2 Score: 58.9214 bits (141), Expect = 1.002e-8
Identity = 66/70 (94.29%), Postives = 67/70 (95.71%), Query Frame = 2 
Query:  449 VPQGCFSLCKVLRKKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXISKLPDNIDKIQYLQVLNVSN 658
            VPQGCFSLCKVLRKKKLFLNNNQIQLLNSNGNNLEDLTLLTDLHLEYNLISKLPDNIDKIQYLQ L+  N
Sbjct:   34 VPQGCFSLCKVLRKKKLFLNNNQIQLLNSNGNNLEDLTLLTDLHLEYNLISKLPDNIDKIQYLQTLDYEN 103
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Match: SMESG000027652.1 (SMESG000027652.1)

HSP 1 Score: 130.954 bits (328), Expect = 3.650e-36
Identity = 65/69 (94.20%), Postives = 66/69 (95.65%), Query Frame = 2 
Query: 2078 MYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEE-TKILFM 2281
            MYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEE T  LF+
Sbjct:    1 MYNRNLRQNLLDTIRQMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEEVTYFLFL 69
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Match: SMESG000009928.1 (SMESG000009928.1)

HSP 1 Score: 138.272 bits (347), Expect = 1.510e-33
Identity = 114/410 (27.80%), Postives = 192/410 (46.83%), Query Frame = 2 
Query: 1235 EQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNK-KICEKEEAIATM-------NEPNCSLPQ----------------------TMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            ++LMN +K  E +   LI +I   N   + +E+L ++IE        +  I    + N  K E+L SM + L    +I   N+L +        D+  V+      K+     L+   Q+ EQK+A++ LQ +KD +  RL + I  +E +L  LS  +  +     D+      + R  LV++L+ L  +K  RE +L+ ++ +ME  R  +Q DFW+ QYQ  LD K       +  D EV+ELL   N  E++  F++    +D +    +DD   + + + +++   L  I + K    K + C    A +         N P  ++P                       T+++  +E ECCICL  E +IL ++CGH+CCC+ CS  + +CPLCR  I + + L
Sbjct:  292 DRLMNAIKYEEINTNELINKIFEFNNKAKKKEELRNKIEERNKSFGDISFI----LTNESKNEILNSMKQLLRSTSRIG--NDLDD--------DEFFVD------KRPQNQNLD---QDQEQKLAYKQLQFEKDPQLSRLRDGIEIIEKKLQQLSVEDIIEGSLATDYN----RKDRTHLVDVLKELQCEKENREKILNSKIDQMESSRSYNQKDFWIAQYQTLLDDK-------AKSDFEVRELLKMANMEEWISVFLKNNFTIDMMLDLNKDDLDQLQIRSESVKSKFLSFINEWKFNPIKSEKCGSPSAPSITLSECPETNSPQPAIPSAPVLETPPRSHDRNTPCNPHENTVVLARKEAECCICLTLEARILLLNCGHVCCCQPCSLTVNQCPLCRQLIVQRVNL 667
[Close]
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Match: SMESG000009928.1 (SMESG000009928.1)

HSP 1 Score: 137.887 bits (346), Expect = 1.779e-33
Identity = 114/410 (27.80%), Postives = 192/410 (46.83%), Query Frame = 2 
Query: 1235 EQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKYHLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDKVIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIYTVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALREIVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKELLSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIRQMKIEQNK-KICEKEEAIATM-------NEPNCSLPQ----------------------TMIMTHRETECCICLNEETKILFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKL 2374
            ++LMN +K  E +   LI +I   N   + +E+L ++IE        +  I    + N  K E+L SM + L    +I   N+L +        D+  V+      K+     L+   Q+ EQK+A++ LQ +KD +  RL + I  +E +L  LS  +  +     D+      + R  LV++L+ L  +K  RE +L+ ++ +ME  R  +Q DFW+ QYQ  LD K       +  D EV+ELL   N  E++  F++    +D +    +DD   + + + +++   L  I + K    K + C    A +         N P  ++P                       T+++  +E ECCICL  E +IL ++CGH+CCC+ CS  + +CPLCR  I + + L
Sbjct:  294 DRLMNAIKYEEINTNELINKIFEFNNKAKKKEELRNKIEERNKSFGDISFI----LTNESKNEILNSMKQLLRSTSRIG--NDLDD--------DEFFVD------KRPQNQNLD---QDQEQKLAYKQLQFEKDPQLSRLRDGIEIIEKKLQQLSVEDIIEGSLATDYN----RKDRTHLVDVLKELQCEKENREKILNSKIDQMESSRSYNQKDFWIAQYQTLLDDK-------AKSDFEVRELLKMANMEEWISVFLKNNFTIDMMLDLNKDDLDQLQIRSESVKSKFLSFINEWKFNPIKSEKCGSPSAPSITLSECPETNSPQPAIPSAPVLETPPRSHDRNTPCNPHENTVVLARKEAECCICLTLEARILLLNCGHVCCCQPCSLTVNQCPLCRQLIVQRVNL 669
The following BLAST results are available for this feature:
BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LRSAM11.964e-4226.50leucine rich repeat and sterile alpha motif contai... [more]
LRSAM11.964e-4226.50leucine rich repeat and sterile alpha motif contai... [more]
LRSAM11.964e-4226.50leucine rich repeat and sterile alpha motif contai... [more]
LRSAM17.982e-4025.84leucine rich repeat and sterile alpha motif contai... [more]
BIRC23.782e-736.17baculoviral IAP repeat containing 2 [Source:HGNC S... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
K11D12.91.023e-640.00pep chromosome:WBcel235:V:5042408:5046203:-1 gene:... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Diap21.551e-738.64gene:FBgn0015247 transcript:FBtr0087297[more]
Diap21.551e-738.64gene:FBgn0015247 transcript:FBtr0087296[more]
mib13.662e-639.13gene:FBgn0263601 transcript:FBtr0075520[more]
Diap13.828e-635.56gene:FBgn0260635 transcript:FBtr0075500[more]
Diap13.828e-635.56gene:FBgn0260635 transcript:FBtr0333618[more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lrsam17.027e-5527.06leucine rich repeat and sterile alpha motif contai... [more]
lrsam12.642e-5427.06leucine rich repeat and sterile alpha motif contai... [more]
lrsam15.531e-5325.63leucine rich repeat and sterile alpha motif contai... [more]
mul1b4.749e-846.00mitochondrial E3 ubiquitin protein ligase 1b [Sour... [more]
mul1b4.749e-846.00mitochondrial E3 ubiquitin protein ligase 1b [Sour... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LRSAM14.481e-6328.51leucine rich repeat and sterile alpha motif contai... [more]
LRSAM11.778e-5628.51leucine rich repeat and sterile alpha motif contai... [more]
LRSAM13.189e-5527.77leucine rich repeat and sterile alpha motif contai... [more]
LRSAM11.902e-5225.39leucine rich repeat and sterile alpha motif contai... [more]
crebrf6.556e-840.43CREB3 regulatory factor [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lrsam19.762e-4627.27leucine rich repeat and sterile alpha motif contai... [more]
Lrsam19.762e-4627.27leucine rich repeat and sterile alpha motif contai... [more]
Birc21.040e-738.30baculoviral IAP repeat-containing 2 [Source:MGI Sy... [more]
Birc21.040e-738.30baculoviral IAP repeat-containing 2 [Source:MGI Sy... [more]
Birc34.833e-738.64baculoviral IAP repeat-containing 3 [Source:MGI Sy... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q80ZI6|LRSM1_MOUSE6.829e-4527.27E3 ubiquitin-protein ligase LRSAM1 OS=Mus musculus... [more]
sp|Q6UWE0|LRSM1_HUMAN9.430e-4226.50E3 ubiquitin-protein ligase LRSAM1 OS=Homo sapiens... [more]
sp|Q94HV7|SPL1P_ARATH1.555e-839.34E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis th... [more]
sp|Q8L7N4|SP1_ARATH2.115e-850.00E3 ubiquitin-protein ligase SP1 OS=Arabidopsis tha... [more]
sp|Q557E7|CBLA_DICDI3.396e-741.30E3 ubiquitin-protein ligase cblA OS=Dictyostelium ... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3M6UJZ93.593e-6829.42Uncharacterized protein OS=Pocillopora damicornis ... [more]
A0A0L8IDQ06.965e-6830.81Uncharacterized protein OS=Octopus bimaculoides OX... [more]
A0A210QV317.324e-6827.43E3 ubiquitin-protein ligase LRSAM1 OS=Mizuhopecten... [more]
A0A564YV182.411e-6429.74Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0R3SW042.155e-6329.66Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lrsam12.128e-5727.71leucine rich repeat and sterile alpha motif contai... [more]
birc23.890e-736.17baculoviral IAP repeat containing 2 [Source:NCBI g... [more]
birc25.237e-736.17baculoviral IAP repeat containing 2 [Source:NCBI g... [more]
mul1b2.356e-638.00mitochondrial E3 ubiquitin protein ligase 1 [Sourc... [more]
mylipa5.330e-642.86myosin regulatory light chain interacting protein ... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
lrsam13.682e-3525.33leucine rich repeat and sterile alpha motif contai... [more]
mul1a2.278e-726.47mitochondrial E3 ubiquitin protein ligase 1a [Sour... [more]
birc29.311e-731.82baculoviral IAP repeat containing 2 [Source:ZFIN;A... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO434234.829e-5726.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO394014.724e-832.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO436951.423e-739.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO303811.602e-736.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO415683.812e-739.13Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lrsam19.789e-4627.17leucine rich repeat and sterile alpha motif contai... [more]
lrsam13.222e-4527.36leucine rich repeat and sterile alpha motif contai... [more]
birc22.297e-736.17baculoviral IAP repeat-containing protein 2 [Sourc... [more]
mul1b2.440e-739.58mitochondrial E3 ubiquitin protein ligase 1 [Sourc... [more]
xiap2.577e-637.29E3 ubiquitin-protein ligase XIAP [Source:NCBI gene... [more]
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BLAST of E3 ubiquitin-protein ligase LRSAM1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046440.11.067e-895.89SMESG000046440.1[more]
SMESG000046440.11.002e-896.20SMESG000046440.1[more]
SMESG000027652.13.650e-3694.20SMESG000027652.1[more]
SMESG000009928.11.510e-3327.80SMESG000009928.1[more]
SMESG000009928.11.779e-3327.80SMESG000009928.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025624 ID=SMED30025624|Name=E3 ubiquitin-protein ligase LRSAM1|organism=Schmidtea mediterranea sexual|type=transcript|length=2468bp
AACTACTTCAATTTCTTCATCTGTTTAGGAAGGAAGTGAAGTTTCTAGGA
CACATAATAAATCAAGATAGGATAAAAACAGACCAAAATACGGTTGAATC
CATAAAATCATTCAGTTATGCCAAGAAGGCGAGAGCATTAGAAAATATAT
GTGGAAGAAATAATGAAAATGAATGAAGCACAGACAAAGACTCAAGTGTT
GGGTATTATCAGACTTCAAAAAAGAATTTATGTTGGACAAGGACGCGAGT
TTCGATACTATAGGAGCTAAAGATGAGAGACACAATAATGTCGGTGTATA
TGGTTCATACGCAATTGAACAGCCACCAAAAAGGATATTGGATCACTATG
CAAGAATTATTGACAATACAATACAAAGATTTGGAGGAGATAAAATCGAT
ACTTTATTGGACTTTAAGCATGAGACTTTTTTAAAGAGTTGTATTAATGT
TCCTCAAGGTTGTTTCTCTTTATGTAAAGTTTTACGGAAAAAAAAGCTAT
TTCTGAATAACAATCAGATTCAACTTTTGAATTCCAATGGGAACAATCTG
GAAGATCTTACACTACTGACAGATTTGCATTTAGAATATAATTTGATTTC
CAAATTACCTGATAATATCGATAAGATTCAATATCTACAAGTTTTAAATG
TCAGTAACAACAATTTGACATGCCTTCCAAATTCTTTGGGAAATCTTTTG
TATTTGAGTCAGCTTCTCATTCAGAATAATCAGATTGAAAAATTGCCACT
TAATCTTTTAACGTCAACAAGCAGTTTAGAAGTAATCGATGTTTCAGAGA
ATCCTAAATTCCATTTTATTCCTATAAATATTATGAATCTCCGAAAATTA
CGGAAATTTAATTTCTCATCTAACTATTTACAATTTCCATCCCAAGATAT
TTGCCAGCAAGGCATTGTTGAAATTCTCAAATCAGTATGCAAAGCCAATC
AAATTCCAATTAATATGTCATTTTTCAACCAATTATCTAACAATGAAGAC
AACGCTGTGGATACAGTGGATTTTTCTACACTTGATTATGAAAACTTTAA
ACGAAATCGATTGGATCTATTAAAATCCATTGAAAGTGAATTAGAAAGTG
TTATGAAATCACAAAAAACATTTGCCGATAAAGCATTACATGAGCGCATG
ATATTTTTCAATACATTAGCCGAAGAGGAAGCTAAAATTACTAATCAAAT
AAATAAAATGCATCTAGAAAGAGATCTTCACAGAGAACAATTGATGAATG
ATGTCAAAGCTGCTGAAGCTGATGCTGCAAATCTTATTGAAGAAATTATG
ACTATAAATCGAACTTGTCAAAATCAAGAAAAATTGCTCGATGAGATAGA
AAGTGAAACAATGAAAAAATATCATTTGTATCAAATTTCAAAAGACGAAA
TTCAAAATTTACGGAAACAAGAAGTCTTGGCTTCTATGTCTGAAAATCTT
TTTCACCAGCGTAAAATTGACAAACAGAATGAACTGAAAGAAATCGCATT
TGCACGTTCTTTGAATGATAAAGTTATTGTAGAAGTGAATAATGAGCGCA
GAAAGAAACTTTCAGAAATGAAGTTAGAATTGGAAAATCAAGAAAATGAG
CAGAAAATAGCTTTTCAGATGCTACAAATACAGAAAGATATTCGTAGTTT
ACGATTAACAGAACATATTTACACTGTCGAGTCAGAATTGATTCTATTAT
CCCAAGCAGAACAGAAAAAACGAGAAGAAAAAGTTGATTTCAAATTCAAT
ATTTTAAATGAGCGACGTCAGCAGCTGGTGGAAATTTTACAAAATTTAAT
CCAACAAAAAGCATTGCGAGAAATTGTGCTATCAAAGAGAATGTTGGAAA
TGGAGAAACAACGCCAACAAGATCAGGATGATTTCTGGCTGATACAATAT
CAGAGATGCTTGGACATGAAACCTCACTATCTTTGGAATGACAGTGGTCT
AGATTTGGAAGTGAAGGAACTTCTAAGTCGAGTAAATGCTACTGAATATT
TACCGTTTTTTATCAGATACAAAACTAGATTGGATCAGTTGGAAAGATTC
ACTGAAGATGATTGGAAAAATGTTGGAATGTATAACAGAAATCTCAGACA
AAATTTGTTGGATACTATAAGACAAATGAAAATTGAACAGAATAAAAAGA
TCTGTGAAAAGGAAGAGGCGATTGCAACAATGAATGAACCGAATTGTTCT
CTACCTCAAACAATGATTATGACTCACAGAGAAACTGAATGTTGTATATG
TTTAAATGAAGAGACAAAAATTTTATTCATGGATTGTGGGCATATTTGCT
GTTGTGAAAAATGTTCAAATAATCTGAAAGAGTGTCCTTTGTGCCGAAAT
GAAATTAAAAAAATAATCAAATTAATGTGTTGGTAGTAATTGATTAATCA
ATACAATAAAAAAGTCTTGGTGTTTTATTGATTTTAAATTGTAATAAATT
TGTTGACAGATATACAGT
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protein sequence of SMED30025624-orf-1

>SMED30025624-orf-1 ID=SMED30025624-orf-1|Name=SMED30025624-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=738bp
MKHRQRLKCWVLSDFKKEFMLDKDASFDTIGAKDERHNNVGVYGSYAIEQ
PPKRILDHYARIIDNTIQRFGGDKIDTLLDFKHETFLKSCINVPQGCFSL
CKVLRKKKLFLNNNQIQLLNSNGNNLEDLTLLTDLHLEYNLISKLPDNID
KIQYLQVLNVSNNNLTCLPNSLGNLLYLSQLLIQNNQIEKLPLNLLTSTS
SLEVIDVSENPKFHFIPINIMNLRKLRKFNFSSNYLQFPSQDICQQGIVE
ILKSVCKANQIPINMSFFNQLSNNEDNAVDTVDFSTLDYENFKRNRLDLL
KSIESELESVMKSQKTFADKALHERMIFFNTLAEEEAKITNQINKMHLER
DLHREQLMNDVKAAEADAANLIEEIMTINRTCQNQEKLLDEIESETMKKY
HLYQISKDEIQNLRKQEVLASMSENLFHQRKIDKQNELKEIAFARSLNDK
VIVEVNNERRKKLSEMKLELENQENEQKIAFQMLQIQKDIRSLRLTEHIY
TVESELILLSQAEQKKREEKVDFKFNILNERRQQLVEILQNLIQQKALRE
IVLSKRMLEMEKQRQQDQDDFWLIQYQRCLDMKPHYLWNDSGLDLEVKEL
LSRVNATEYLPFFIRYKTRLDQLERFTEDDWKNVGMYNRNLRQNLLDTIR
QMKIEQNKKICEKEEAIATMNEPNCSLPQTMIMTHRETECCICLNEETKI
LFMDCGHICCCEKCSNNLKECPLCRNEIKKIIKLMCW*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR003591Leu-rich_rpt_typical-subtyp
IPR013083Znf_RING/FYVE/PHD
IPR032675LRR_dom_sf
IPR013761SAM/pointed_sf
IPR001611Leu-rich_rpt
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0016874ligase activity