Repressor of the inhibitor of the protein kinase

Overview
NameRepressor of the inhibitor of the protein kinase
Smed IDSMED30025610
Length (bp)3520
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Repressor of the inhibitor of the protein kinase (SMED30025610) t-SNE clustered cells

Violin plots show distribution of expression levels for Repressor of the inhibitor of the protein kinase (SMED30025610) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Repressor of the inhibitor of the protein kinase (SMED30025610) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Repressor of the inhibitor of the protein kinase (SMED30025610) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025610

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30025610SMESG000067685.1 Contig3727GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30025610SMESG000079624.1 SMESG000079569.1 Contig3727GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30025610SMESG000042333.1 Contig332uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30025610SMESG000042333.1 Contig332newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025610SMESG000079624.1 dd_Smed_v4_6771_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30025610 dd_Smed_v4_1325_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025610 dd_Smed_v4_1325_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30025610 dd_Smed_v4_1325_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Zebrafish
Match: si:ch211-240b21.2 (si:ch211-240b21.2 [Source:ZFIN;Acc:ZDB-GENE-060526-125])

HSP 1 Score: 55.8398 bits (133), Expect = 5.995e-8
Identity = 31/75 (41.33%), Postives = 46/75 (61.33%), Query Frame = 1 
Query: 1822 EDMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEINI----NKIIDRFA 2034
            E++F     L  I  T P+  + A + FSTLKR+K++L+N MT+ERL  LA+LSI   L   +     K+I++FA
Sbjct:  147 EEVFSETVTLVKILVTTPMTTAEAERCFSTLKRIKTFLRNSMTQERLNALAMLSIEKRLVTEMTDFNQKVIEKFA 221
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Match: J9KEM1 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 6.868e-12
Identity = 40/73 (54.79%), Postives = 57/73 (78.08%), Query Frame = 1 
Query: 1825 DMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARIK 2043
            D++ +  QL I  YTLPV+V+SA + FSTL+RLK++L++RM EERL GLALL I  ++++N+  IIDRFA+ K
Sbjct: 1017 DIYPTVKQLLITIYTLPVSVASAERSFSTLRRLKTWLRSRMGEERLTGLALLHIHRDIQLNVEDIIDRFAKEK 1089
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Match: T1EL50 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20197305 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.668e-11
Identity = 36/76 (47.37%), Postives = 56/76 (73.68%), Query Frame = 1 
Query: 1834 LSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARIKDGKHEF 2061
            ++Q    II    PV+V+SA + FSTL+R+K++L+ RMTE+RLVGLALL++  ++ +N+  II+RFA+  + K EF
Sbjct:    3 ITQKLFRIIK-ACPVSVASAERSFSTLRRIKTWLRTRMTEDRLVGLALLNVHRDVLVNVENIIERFAKSGNRKIEF 77
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Match: T1G5M7 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20216374 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.956e-11
Identity = 35/79 (44.30%), Postives = 56/79 (70.89%), Query Frame = 1 
Query: 1825 DMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARIKDGKHEF 2061
            +++L+   L  I  T+PV+V++A + FSTL+RLK++++++M EERL GLAL++I  + E++I  II RF R K  K  F
Sbjct:   18 NLYLTIHSLLSILCTMPVSVATAERSFSTLRRLKTWMRSKMKEERLTGLALMNIHCDTELDIEDIITRFGRKKCRKMTF 96
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Match: T1EL54 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20197304 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.561e-11
Identity = 32/63 (50.79%), Postives = 51/63 (80.95%), Query Frame = 1 
Query: 1873 PVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARIKDGKHEF 2061
            PV+V+SA + FSTL+R+K++L+ RMTE+RLVGLALL++  ++ +N+  +I+RFA+  + K EF
Sbjct:   15 PVSVASAERSFSTLRRIKTWLRTRMTEDRLVGLALLNVHRDVLVNVENVIERFAKSGNRKIEF 77
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Match: X1XEX7 (Dimer_Tnp_hAT domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.200e-11
Identity = 33/64 (51.56%), Postives = 49/64 (76.56%), Query Frame = 1 
Query: 1852 AIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARIK 2043
             +I  TLPV+ SSA + FSTL+RLK+YL+N  +E+RL GLALLS+   ++I+I +II+ F+R+ 
Sbjct:   11 CLIFATLPVSTSSAERSFSTLRRLKTYLRNSCSEDRLTGLALLSVHRGIQIDIEEIINTFSRMP 74
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Cavefish
Match: ENSAMXT00000055782.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001476.1:1798:4262:-1 gene:ENSAMXG00000042376.1 transcript:ENSAMXT00000055782.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.7582 bits (125), Expect = 2.446e-6
Identity = 27/61 (44.26%), Postives = 43/61 (70.49%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSI--R*NLEININKIIDRFARIK 2043
            TLP+ V+S  + FS+LK +K++L++ M++ERL GLA++SI  R    +N+  II+ FA  K
Sbjct:  678 TLPITVASGERSFSSLKLIKNFLRSTMSQERLTGLAMISIEQRVRRSLNMEDIINAFAETK 738
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Cavefish
Match: ENSAMXT00000057644.1 (pep primary_assembly:Astyanax_mexicanus-2.0:10:17620682:17622807:-1 gene:ENSAMXG00000043641.1 transcript:ENSAMXT00000057644.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 2.659e-6
Identity = 28/74 (37.84%), Postives = 47/74 (63.51%), Query Frame = 1 
Query: 1822 EDMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSI--R*NLEININKIIDRFAR 2037
            +++F    +L  +  ++PV  +S  + FSTLK +K+YL++ MT+ERL  L +LSI  R    +N+ + +D FAR
Sbjct:  549 KEVFFELYKLCKVAVSIPVTTASCERSFSTLKLVKTYLRSTMTDERLSNLGVLSIESRRAKTLNLEEFVDLFAR 622
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Cavefish
Match: ENSAMXT00000032220.1 (pep primary_assembly:Astyanax_mexicanus-2.0:24:13450075:13452201:1 gene:ENSAMXG00000029800.1 transcript:ENSAMXT00000032220.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 3.043e-6
Identity = 28/74 (37.84%), Postives = 47/74 (63.51%), Query Frame = 1 
Query: 1822 EDMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSI--R*NLEININKIIDRFAR 2037
            +++F    +L  +  ++PV  +S  + FSTLK +K+YL++ MT+ERL  L +LSI  R    +N+ + +D FAR
Sbjct:  570 KEVFFELYKLCKVAVSIPVTTASCERSFSTLKLVKTYLRSTMTDERLSNLGVLSIESRRAKTLNLEEFVDLFAR 643
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Cavefish
Match: ENSAMXT00000025204.2 (pep primary_assembly:Astyanax_mexicanus-2.0:12:7364240:7371214:-1 gene:ENSAMXG00000024490.2 transcript:ENSAMXT00000025204.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 3.723e-6
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NL--EININKIIDRFARIKDGKHEF 2061
            TLPV+V+S  + FS LK +K+Y+++ M +ERL GLAL+SI   +   +++  I+  FA  K  K +F
Sbjct:  667 TLPVSVASGERSFSALKLIKTYMRSTMGQERLTGLALMSIEREVCRSLDMEDIVVAFAEAKVHKQKF 733
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Match: EDO35158 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMB6])

HSP 1 Score: 55.8398 bits (133), Expect = 3.290e-9
Identity = 24/58 (41.38%), Postives = 45/58 (77.59%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARI 2040
            TLP++ +S  +  S+L+ LK+YL++ MTE+RL GLAL+    ++++++++IID FA++
Sbjct:   60 TLPISSASCERSISSLRNLKTYLRSTMTEDRLNGLALMYTHKDMDLDLDRIIDSFAQL 117
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Match: EDO29399 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMB6])

HSP 1 Score: 55.8398 bits (133), Expect = 3.290e-9
Identity = 24/58 (41.38%), Postives = 45/58 (77.59%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARI 2040
            TLP++ +S  +  S+L+ LK+YL++ MTE+RL GLAL+    ++++++++IID FA++
Sbjct:   60 TLPISSASCERSISSLRNLKTYLRSTMTEDRLNGLALMYTHKDMDLDLDRIIDSFAQL 117
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Match: EDO32811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU05])

HSP 1 Score: 55.0694 bits (131), Expect = 5.877e-9
Identity = 24/58 (41.38%), Postives = 45/58 (77.59%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARI 2040
            TLP++ +S  +  S+L+ LK+YL++ MTE+RL GLAL+    ++++++++IID FA++
Sbjct:   60 TLPISSASCERSISSLRNLKTYLRSTMTEDRLNGLALMYTHKDMDLDLDRIIDLFAQL 117
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Match: EDO32247 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVL6])

HSP 1 Score: 55.0694 bits (131), Expect = 6.536e-9
Identity = 24/58 (41.38%), Postives = 45/58 (77.59%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARI 2040
            TLP++ +S  +  S+L+ LK+YL++ MTE+RL GLAL+    ++++++++IID FA++
Sbjct:   60 TLPISSASCERSISSLRNLKTYLRSTMTEDRLNGLALMYTHKDMDLDLDRIIDLFAQL 117
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Match: EDO33687 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRH2])

HSP 1 Score: 54.6842 bits (130), Expect = 6.610e-9
Identity = 24/58 (41.38%), Postives = 45/58 (77.59%), Query Frame = 1 
Query: 1867 TLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NLEININKIIDRFARI 2040
            TLP++ +S  +  S+L+ LK+YL++ MTE+RL GLAL+    ++++++++IID FA++
Sbjct:   58 TLPISSASCERSISSLRNLKTYLRSTMTEDRLNGLALMYTHKDMDLDLDRIIDLFAQL 115
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Medaka
Match: ENSORLT00000028133.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948955])

HSP 1 Score: 53.5286 bits (127), Expect = 1.280e-6
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 1 
Query: 1858 IHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NL--EININKIIDRFARIKDGKHEF 2061
            I  TLPV V+ A + FS LK +KSYL++ M++ERL  LA++SI  ++  +I+ + +ID FA  K  K  F
Sbjct:  782 IALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKVRF 851
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Medaka
Match: ENSORLT00000042294.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:110016639])

HSP 1 Score: 53.5286 bits (127), Expect = 1.280e-6
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 1 
Query: 1858 IHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NL--EININKIIDRFARIKDGKHEF 2061
            I  TLPV V+ A + FS LK +KSYL++ M++ERL  LA++SI  ++  +I+ + +ID FA  K  K  F
Sbjct:  782 IALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKVRF 851
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Medaka
Match: ENSORLT00000045044.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948193])

HSP 1 Score: 53.5286 bits (127), Expect = 1.280e-6
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 1 
Query: 1858 IHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSIR*NL--EININKIIDRFARIKDGKHEF 2061
            I  TLPV V+ A + FS LK +KSYL++ M++ERL  LA++SI  ++  +I+ + +ID FA  K  K  F
Sbjct:  782 IALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKVRF 851
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Match: SMESG000041938.1 (SMESG000041938.1)

HSP 1 Score: 67.781 bits (164), Expect = 7.812e-23
Identity = 39/73 (53.42%), Postives = 53/73 (72.60%), Query Frame = 1 
Query: 1753 LKKFFQQSDCLASXXXXXXXXXGEDMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGL 1971
             K    QS CL   LE+IILI  +DM  + SQLA+I YTLPV+V+S  +IF TLKRLK++L+N+M +ER+ G+
Sbjct:  301 FKNSIHQSGCLVPTLEQIILIFNKDMLPALSQLALIPYTLPVSVASTERIFFTLKRLKTFLRNQMADERIFGV 373

HSP 2 Score: 60.077 bits (144), Expect = 7.812e-23
Identity = 31/48 (64.58%), Postives = 39/48 (81.25%), Query Frame = 3 
Query: 1614 FIPKNLITLDEPALLQNINQILEKFAIVMKKDVIILKYQFSQELQLFK 1757
            FI KNL TLDE  LLQNINQI E FAI++ KD +ILK+Q S++L++FK
Sbjct:  255 FISKNLSTLDEFFLLQNINQISENFAIMLNKDSLILKFQLSEDLKIFK 302
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Match: SMESG000079579.1 (SMESG000079579.1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.279e-7
Identity = 23/25 (92.00%), Postives = 24/25 (96.00%), Query Frame = 1 
Query: 3058 VVANQESAGTGKDKPAASDWSIGYY 3132
            +VANQESAGTGKDKP ASDWSIGYY
Sbjct:  168 IVANQESAGTGKDKPDASDWSIGYY 192
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Match: SMESG000079624.1 (SMESG000079624.1)

HSP 1 Score: 52.373 bits (124), Expect = 4.884e-7
Identity = 58/105 (55.24%), Postives = 67/105 (63.81%), Query Frame = 1 
Query: 3073 ESAGTGKDKPAASDWSIGYYXXXXXXXXXXXXXXXXXXXXXXLXXXXXXXXXXXXXXXESQQTTDEQASSIMDETENGFPEFWERSDYTKDENDFKNYQRLGKCG 3387
            ESAGTGKDKPAASDWSIGYY    I I+  I+++ +T+ICVILNIVRNRNCRRD VN ESQQ+ ++Q                         ND KNYQRL KCG
Sbjct:  139 ESAGTGKDKPAASDWSIGYY----IGILIAILILIVTIICVILNIVRNRNCRRDGVNHESQQSINKQG------------------------NDLKNYQRLRKCG 215
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Match: SMESG000079624.1 (SMESG000079624.1)

HSP 1 Score: 52.373 bits (124), Expect = 6.363e-7
Identity = 58/105 (55.24%), Postives = 67/105 (63.81%), Query Frame = 1 
Query: 3073 ESAGTGKDKPAASDWSIGYYXXXXXXXXXXXXXXXXXXXXXXLXXXXXXXXXXXXXXXESQQTTDEQASSIMDETENGFPEFWERSDYTKDENDFKNYQRLGKCG 3387
            ESAGTGKDKPAASDWSIGYY    I I+  I+++ +T+ICVILNIVRNRNCRRD VN ESQQ+ ++Q                         ND KNYQRL KCG
Sbjct:  159 ESAGTGKDKPAASDWSIGYY----IGILIAILILIVTIICVILNIVRNRNCRRDGVNHESQQSINKQG------------------------NDLKNYQRLRKCG 235
[Close]
BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Match: SMESG000070554.1 (SMESG000070554.1)

HSP 1 Score: 49.2914 bits (116), Expect = 4.183e-6
Identity = 34/83 (40.96%), Postives = 49/83 (59.04%), Query Frame = 1 
Query: 1819 GEDMFLSQSQLAIIHYTLPVNVSSANQIFSTLKRLKSYLKNRMTEERLVGLALLSI-R*NLE-ININKIIDRFARIKDGKHEF 2061
            GED+F +      +  T+ V+++S  + FS LK + SYL+  M +ERL  LAL SI R  L+ IN + +ID FA +K  K  F
Sbjct:  116 GEDVFPNLRIAQQMLLTIAVSIASCERSFSKLKLILSYLRESMGQERLDSLALFSIERDTLDSINFDDVIDTFAAVKARKIYF 198
The following BLAST results are available for this feature:
BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:ch211-240b21.25.995e-841.33si:ch211-240b21.2 [Source:ZFIN;Acc:ZDB-GENE-060526... [more]
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
J9KEM16.868e-1254.79Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
T1EL502.668e-1147.37Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
T1G5M72.956e-1144.30Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
T1EL544.561e-1150.79Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
X1XEX76.200e-1151.56Dimer_Tnp_hAT domain-containing protein OS=Acyrtho... [more]
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSAMXT00000055782.12.446e-644.26pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000057644.12.659e-637.84pep primary_assembly:Astyanax_mexicanus-2.0:10:176... [more]
ENSAMXT00000032220.13.043e-637.84pep primary_assembly:Astyanax_mexicanus-2.0:24:134... [more]
ENSAMXT00000025204.23.723e-641.79pep primary_assembly:Astyanax_mexicanus-2.0:12:736... [more]
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO351583.290e-941.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO293993.290e-941.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO328115.877e-941.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO322476.536e-941.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO336876.610e-941.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Repressor of the inhibitor of the protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000028133.11.280e-644.29zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000042294.11.280e-644.29zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000045044.11.280e-644.29zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
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BLAST of Repressor of the inhibitor of the protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041938.17.812e-2353.42SMESG000041938.1[more]
SMESG000079579.13.279e-792.00SMESG000079579.1[more]
SMESG000079624.14.884e-755.24SMESG000079624.1[more]
SMESG000079624.16.363e-755.24SMESG000079624.1[more]
SMESG000070554.14.183e-640.96SMESG000070554.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025610 ID=SMED30025610|Name=Repressor of the inhibitor of the protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=3520bp
GATAGTGTGTATGACATAATGATTGAAATTAATAAAACTTAAATCGTTTC
CTTTAAAATGATAGAATTCGTCTCCATTAATTCCTTTCGCAAAAAATACA
TGTGCCCATTTTCTTTTATAATTGTTTAGTTTTTTTCTTTAGATAATTAT
TCAGGTTTGTACTATTTATCTATAATATTAGAGTAGCCTATTTATTTTGA
TACATTTTGAAATTTTACCCTTCTCATTGCTTGAACATTTTTTTGATTTA
TATCAAATTTTTATATTTGTGGTTAATTATGATTGAGCAAATCTAAAATG
TAAAAATATGTGAATACTTTGAATGTTTTGACCTTCCATGGATGAACCTT
TGAAGGTTGAAGTAACACCATAATATAGGACATCCACGATTATATCTACA
CACCAAAGATTGGGATGGGAATAGTTTGAGAAACTTATGATTTAAAGGAA
ATCAAAAATATAAAACTGATTTTGACACACCATAATTCAACCAAGAGAAC
TCTGGTACAACTATGATATACGCAACATAAACAATTCTTGCATTTTTCTC
TTTTCATATTTTGTTTTTTTGTTTTTCAGTAGTATTTTGATAATTAATCA
TTTTTGCACTATTTTCTCCATAAATCTGGCACCAGTGAGATCAAGGACGG
ATCCAGGGCATGAGCGATAAGGGCGATCGCCTCCCAAAGAAGATTATGAA
GTTTCTTTCTTGGAATTAAAATTAGTTAAAATAAGACAATCGTCCTAATT
TTTGTGTGCATAAATGTATATATTACTGATCAGGAAATAAACAAGATAGG
AAAATAATCATCATATATTAGAGTTAATTTTTACAGTGTAGGCTACACTA
GATGGACCTGACAACTTTAACAAAAACAAATCGTTATTTTATTGCACTCA
AACAGGGTTCGTTTTAATCCAATAAAATGTGTTTTTGTTTTGTGTTATTT
GAGACATGTGTTATTTCATGTTTAAAGATTGACATTTTTCTGTACAATGA
AATATATTTAAAGTAAAAAAATTATGGAAAGATTTTCTAATGAGTTTAAC
TACCTGAGCCAGTGTAAAAATATTCGTTATATCGTATTCTGCACTGGAAT
CGACGTCATTAGTGACTTTTTCAGTAGTCTTTAAACTTTTATTGAAATTC
TTAATGAAATTTCTGACTGGTAAGATATTACTGATTCCAGCAAATCTCGA
TTGTTAACAGCGATTTTAACTTGATTTGAATTTTTAATTGCCTTGCATTG
CCAAGATTCCGTGCTCCATTTGCTTATACCTTTGTGCAATATCTTTCAAA
AAACTTCCCTTGATGTTTTACGTCACCAACATTTAATTGATTCGTTGACT
GAAACTTCGAAGGAGATGCGAAACAACTGCGACTTAGACTTAAAAATTAT
TTTTCCAAATGCTTTGTACATTTGCAATAAACTAGATATCTGTTGAAATA
CCAAGACAAACTAAACGACAAAGCAATATTAGTAATGTTCTTAAATTTGA
ATCAAAAGATTATTACAAGAAGACAATAATTATCCTAATGCTCGAAAAAA
TTATAATGGAACATATTACAGATCACTAGAGAAAAATTGACAAAGTTTTT
GTTCATCAGCATTTTTATTCCCAAAAACTTGATAACTCTTGACGAACCTG
CTCTCCTTCAAAACATCAATCAAATCTTAGAAAAATTCGCGATAGTGATG
AAGAAGGACGTAATCATATTAAAATATCAGTTTTCTCAAGAATTGCAATT
ATTTAAAAAAGTTTTTTCAGCAATCTGATTGTTTAGCCTCAATTTTAGAG
GAAATCATTTTAATATAAGGTGAGGACATGTTCCTCTCTCAATCTCAATT
AGCTATTATCCACTACACTTTACCAGTAAATGTTTCTTCCGCCAACCAAA
TCTTTTCCACTCTGAAAAGGTTAAAATCTTATTTGAAAAATCGAATGACT
GAAGAAAGATTAGTAGGATTGGCATTACTTAGTATTCGTTGAAATTTGGA
AATTAATATTAACAAGATAATTGACCGATTTGCAAGGATTAAAGACGGAA
AACATGAATTTAAATTCAATTAAAAACGTTAAGATTATAGTACAACTTTA
AATGATAAATAATAATTTGTATTTTAAAAAAGTTTAATGTTAAAAATTTA
AAAATACTAAATTTTAATACGCAAAATGTGATATCCGTGTAAATAATCAA
AATTTTAAAACGAGATGAAAAAAACGAGTAATAAATGTTTGGTAATAAAT
AAAAAATTGTTTCTTTTATCAAAACACAAAAGATATACACTAATAGTCTA
ACACGCAAACCTACATAAATTTTCAAGTTGTCACCCCCCAAAAAAATCCT
GGATCTGTCCCTGAGTAAAATGCCCATTTATGGTGATAATTTTTCAAAAC
TTGAACAATTTTTTGTCTTAATCTTTATATTTGTGGTTAATTGTACTGAT
GTCTTCCATATTTCTGAATAGTTAATTGATAGTGGAGCTCAATGATTCAT
TTGACGTCCTACCAATAATAAATCTGACATGTGATCAATTAGTAAATTTA
TGTCTAAAGTAATTTATCCGTAACAAACTGCAAAATCATTCAAATATTCA
TACTAAATGATTTATTAAGCTTTTGAAGGAATAGAAACAGTATAATACAT
GATAGGTCATATTGCAGGTGCTGTTTGGTACCCTCCTCCCCAACAAGGAA
GGATTATTAGCATTAAATCCAAAAAAGTTGAATATCATTTTGTCACATAG
AAAAGCGTGGCAATCATTAATGAAAGCTTTATAGTTTCAACAAGTTTTAT
GAATTATTTAATTGTAGGAAATAAAATCCGTTTCACTTGTTTCGGATCTA
ATTGAAAATGAAATCTGGAAATAAAATATTCTTACTAAGAGGTTTGAATG
GAGGTTTTGCAATCATTTATTTAGTTGACTTGAATTGCGTGATTATTACC
TGTAAACAGCAACATTTATTATGAGATTTATCATTTGGATGGACTTTTCT
ATTTTATGCTTCTCACTTTATTAAAAACAATTAAAAATGACAAATATTTA
TGTAATAGTTGTTGCTAATCAAGAGTCAGCAGGAACTGGCAAGGATAAAC
CGGCTGCGAGTGATTGGTCAATTGGTTATTATATCGGTATTCCAATAGCA
ATTATTGCCATTATAATATTAATTGCCATTACAATGATATGTGTAATCTT
GAATATTGTGAGAAATAGAAATTGCCGACGGGACCGGGTGAATGATGAAT
CTCAACAGACAACCGATGAACAAGCGTCTTCTATAATGGACGAAACTGAG
AATGGTTTTCCAGAATTTTGGGAAAGATCTGACTATACTAAAGATGAGAA
TGATTTTAAAAACTATCAAAGATTGGGGAAATGTGGAAAATACGTAAGAA
AAAGAAATAAATCCAAAAGAAGCAAAGTTTCAACATTATAATATTCAATT
CAAAATATAATTTTACTTTATTATTTGATCTGCATCCAAATCATGTTTTT
ATATAAATTTTCATTTTGCC
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protein sequence of SMED30025610-orf-1

>SMED30025610-orf-1 ID=SMED30025610-orf-1|Name=SMED30025610-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=135bp
MTNIYVIVVANQESAGTGKDKPAASDWSIGYYIGIPIAIIAIIILIAITM
ICVILNIVRNRNCRRDRVNDESQQTTDEQASSIMDETENGFPEFWERSDY
TKDENDFKNYQRLGKCGKYVRKRNKSKRSKVSTL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016301kinase activity
GO:0046983protein dimerization activity
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR008906HATC_C_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 32..54