SMED30025598
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025598 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of SMED30025598 vs. Planmine SMEST
Match: SMESG000050315.1 (SMESG000050315.1) HSP 1 Score: 1004.2 bits (2595), Expect = 0.000e+0 Identity = 519/520 (99.81%), Postives = 520/520 (100.00%), Query Frame = 2 Query: 95 MKFFYEIQLTSIIFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEKDTVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKKANQFFGYPIPNGYKSNKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCSDFSGLMNFNFTEYPTKDITKQGKRGQSLTETGTIFENSLKHSATTAPSTSFHKXXXXXXXXXXXHKLNDFEMDTVKXXXXXXXXXXXXXXXMVTFYGIIRKEMRSHFKKINSLPRVKNNARDLPLPDVSIPLRNVYEFEEETSKELRNSGEYEELY 1654 MKFFYEIQLTSIIFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEKDTVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKKANQFFGYPIPNGYKSNKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYC+DFSGLMNFNFTEYPTKDITKQGKRGQSLTETGTIFENSLKHSATTAPSTSFHKIINNINNNISNHKLNDFEMDTVKIAVIAGIILIVLLLIMVTFYGIIRKEMRSHFKKINSLPRVKNNARDLPLPDVSIPLRNVYEFEEETSKELRNSGEYEELY Sbjct: 1 MKFFYEIQLTSIIFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEKDTVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKKANQFFGYPIPNGYKSNKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCNDFSGLMNFNFTEYPTKDITKQGKRGQSLTETGTIFENSLKHSATTAPSTSFHKIINNINNNISNHKLNDFEMDTVKIAVIAGIILIVLLLIMVTFYGIIRKEMRSHFKKINSLPRVKNNARDLPLPDVSIPLRNVYEFEEETSKELRNSGEYEELY 520
BLAST of SMED30025598 vs. Planmine SMEST
Match: SMESG000050299.1 (SMESG000050299.1) HSP 1 Score: 266.159 bits (679), Expect = 1.921e-81 Identity = 187/531 (35.22%), Postives = 281/531 (52.92%), Query Frame = 2 Query: 119 LTSIIFINVIY--ANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEKD----TVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKK---ANQFFGYPIPNGYKSNK--TFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLR--SLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCSDFSGLMNFNFTEYPTKDITKQGKRGQSLTETGTIFENSLKHSATTAPSTSFHKXXXXXXXXXXXHKLNDFEMDTVKXXXXXXXXXXXXXXXMVTFYGIIRKEMRSHFKKIN------SLPRVKNNARDLPLPDVSIPLRNVYEFE-EETSKELRNSGEYEEL 1651 S+IF I+ NY + +++G+LI + + NFS+FVK F G+V Y ID N +LIE+E++ + K N+ + S+ LCGC C +Y TFIL+ + + +KCN T TL V + K K +QF YP+PN Y K T+++MGNIG +KG N M Y PL QLIGCLD F+ NGKLI+ S ++F C Y +T+ + N ++ TV YD+ DCSISSLN RYC NL +L T ++ +K K IRL+W+ + + CLME+ L +G+ I+F+++ Q + K + ++TL NST + ANFT T+ +++L S+E+I++F+ L + +N G S + C F G M+F +Y + SL T + L ST + K ++ ++ + I I + +V + +++ FY IIRKEMRSH +KI+ P N R+ P NVYE+E E R+SG YE L Sbjct: 8 FVSVIFRLTIFLSTNYIHLELDGDLISQETTNFSLFVKHFKGIVFYGIDRSNKVLIELEEEKDEKRILFKFNNILINEKSRNLCGCPKCSDVYMTFILQLDRSQIAVKCNNQTQTLQVDRSKSKILKPDQFDNYPVPNMYNREKKVTYIFMGNIGPYKGPNYMEYLPLYNQLIGCLDKFYFNGKLIRVTSNSTKFKSFCLTEYIETVHSENFTTKFTVSYDIPDCSISSLNTRYCIRNLSIFNLTTSNTPLKFAGKHFFIRLDWIVSIRYQNLCLMEIFLNDGSAIIFYVENAQSLVVQKVYKNGTFSLSKILETLDTNSTYTMIANFTETKFIVRLSSKEDISTFIQQLNNPPVSINIGISRTQSICKTFEGFMDF---QYKILYSPNKMSSTPSLKPTTALSSRGLDKIKENITST--------VQYTTIVFKKDEIKITSANIIFITISVFLVFIFLLLLFYIIIRKEMRSHIRKISFGTMSAVDPSQSENIRN--------PRPNVYEYEFENVYVSSRDSGGYEIL 519
BLAST of SMED30025598 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1) HSP 1 Score: 204.527 bits (519), Expect = 4.688e-59 Identity = 117/340 (34.41%), Postives = 181/340 (53.24%), Query Frame = 2 Query: 215 IFVKQFSGLVLYAIDSFNNLLIEIEKD---TVFIKLNDTISLA-NSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGKIKKKANQFFGYPIPNGYKS--NKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSL--NTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCSDFSGLMNF 1210 +F+K +G+VLYA+D + +LIE+EK T + N+ S+ N +CGC C +IY + ++ ++ C T L + ++ AN+ YP+P Y + N+TF+ +GNIG F+G ++Y PL+ QL+GCL +ING I F S GS F+ +C Y +TIC +C++S+V Y + DCSIS LN RYC+++L N H LN L RL+W+ + CL+E +++NG I F+ + I+N + H K L S + FTG ++IKL S E+I ++ P+ E + G YC F G +NF Sbjct: 1 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCLECSYIYMEWRIELIKRRPSVTCGNVTQDLITVESRENANKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSH---YLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRV---HYHIPKLLHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINF 334
BLAST of SMED30025598 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1) HSP 1 Score: 204.912 bits (520), Expect = 5.197e-59 Identity = 123/367 (33.51%), Postives = 194/367 (52.86%), Query Frame = 2 Query: 131 IFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEK-----DTVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGK--IKKKANQFFGYPIPNGYKSNKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCSDFSGLMNF 1210 I IN A+Y YQ++ +L NFS+F+K +G+VLY +D + +LIE+EK + VF +I N +CGC +C +IY + ++ L+I C + TL + + + YP Y NKT + +GNIG F +MIY PLK QL+GCL ++N I F S GS F+ +CP Y +TIC N+C+QS + Y +NDCSIS LN +YC+T+L+ + + + L + R++W N + CL++ K+K+G+ I+F++ + I N ++K+L NS + F +I+I+L S E++ + + + E + G SS C F G MNF Sbjct: 5 IKINENSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIR-RNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY-LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRL-IEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCS--DIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNF 366
BLAST of SMED30025598 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1) HSP 1 Score: 204.912 bits (520), Expect = 6.728e-59 Identity = 120/360 (33.33%), Postives = 191/360 (53.06%), Query Frame = 2 Query: 152 ANYTYQDMEGNLIREGSFNFSIFVKQFSGLVLYAIDSFNNLLIEIEK-----DTVFIKLNDTISLANSQELCGCSSCKHIYATFILKTRDNILTIKCNGYTSTLAVGK--IKKKANQFFGYPIPNGYKSNKTFLWMGNIGLFKGLNDMIYFPLKGQLIGCLDNFFINGKLIQFKSYGSQFSFTCPKGYNQTICNGNHCSQSTVFYDMNDCSISSLNGRYCSTNLRSLNTEHSSIKLNHKLSLIRLEWMTNDDLADPCLMEMKLKNGNIILFHLDKTQRFKMIINNKQAKLFHTTQMKTLPINSTNILAANFTGTEILIKLYSEENINSFVLPLVDEVQLVNFGKSSMKGYCSDFSGLMNF 1210 A+Y YQ++ +L NFS+F+K +G+VLY +D + +LIE+EK + VF +I N +CGC +C +IY + ++ L+I C + TL + + + YP Y NKT + +GNIG F +MIY PLK QL+GCL ++N I F S GS F+ +CP Y +TIC N+C+QS + Y +NDCSIS LN +YC+T+L+ + + + L + R++W N + CL++ K+K+G+ I+F++ + I N ++K+L NS + F +I+I+L S E++ + + + E + G SS C F G MNF Sbjct: 23 ASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIR-RNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY-LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRL-IEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCS--DIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNF 377 The following BLAST results are available for this feature:
BLAST of SMED30025598 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30025598 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30025598 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30025598 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30025598 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025598 ID=SMED30025598|Name=SMED30025598|organism=Schmidtea mediterranea sexual|type=transcript|length=1819bpback to top protein sequence of SMED30025598-orf-1 >SMED30025598-orf-1 ID=SMED30025598-orf-1|Name=SMED30025598-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=521bp MKFFYEIQLTSIIFINVIYANYTYQDMEGNLIREGSFNFSIFVKQFSGLVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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