Ccr4-not transcription complex, putative

Overview
NameCcr4-not transcription complex, putative
Smed IDSMED30025587
Length (bp)2897
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ccr4-not transcription complex, putative (SMED30025587) t-SNE clustered cells

Violin plots show distribution of expression levels for Ccr4-not transcription complex, putative (SMED30025587) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ccr4-not transcription complex, putative (SMED30025587) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ccr4-not transcription complex, putative (SMED30025587) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025587

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30025587SMESG000012074.1 dd_Smed_v4_14012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025587SMESG000012074.1 Contig55068newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025587SMESG000012074.1 Contig55068uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
reproductive organSMED30025587SMESG000012074.1 Contig55068newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30025587SMESG000012074.1 Contig55068uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Match: A0A0R3TFA2 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS5739 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.066e-20
Identity = 40/58 (68.97%), Postives = 46/58 (79.31%), Query Frame = 2 
Query: 2264 KTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            K  + K +RAC FY+RG CKKEDCE+AHDL+KVTCKFWE G+CFK  TCPF HGY  E
Sbjct:   36 KPVIAKWRRACSFYLRGHCKKEDCEFAHDLTKVTCKFWEVGECFKGPTCPFLHGYPPE 93
[Close]
BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Match: A0A3S4ZW84 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS14912 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 4.356e-20
Identity = 43/61 (70.49%), Postives = 49/61 (80.33%), Query Frame = 2 
Query: 2255 SIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            S+ +  L K +RAC FY+RG CKKEDCE+AHDLSKVTCKFWE G+CFK DTCPF HGY  E
Sbjct:  142 SVTRPPLAKWRRACSFYLRGHCKKEDCEFAHDLSKVTCKFWEAGECFKGDTCPFLHGYPVE 202
[Close]
BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Match: A0A267EXZ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024248g1 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.129e-19
Identity = 40/63 (63.49%), Postives = 49/63 (77.78%), Query Frame = 2 
Query: 2237 LRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHG 2425
            +R+ PM    T L+KS+RAC F +RG CKKEDCE+AHDL++V CKFW  G+CFK DTCPF HG
Sbjct:   54 VRRQPM----TPLEKSKRACSFLLRGRCKKEDCEFAHDLNRVICKFWSAGECFKGDTCPFLHG 112
[Close]
BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Match: A0A183RJP4 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 6.191e-19
Identity = 43/70 (61.43%), Postives = 51/70 (72.86%), Query Frame = 2 
Query: 2234 SLRQPPMSII--KTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            SL  P   ++  K  + K +RAC FY+RG CKKEDCE+AHDL+KVTCKFWE G+CFK  TCPF HGY  E
Sbjct:  143 SLLYPGTELVNGKPVVAKWRRACSFYLRGHCKKEDCEFAHDLTKVTCKFWEMGECFKGSTCPFLHGYPPE 212
[Close]
BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Match: A0A183M219 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS10093 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.288e-18
Identity = 43/73 (58.90%), Postives = 53/73 (72.60%), Query Frame = 2 
Query: 2225 SNMSLRQPPMSII--KTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            S++S   P   ++  K  + K +RAC FY+RG CKKEDCE+AHDL+KVTCKFWE G+CFK  TCPF HGY  E
Sbjct:   69 SSLSSLYPGTELVNGKPVVAKWRRACSFYLRGHCKKEDCEFAHDLTKVTCKFWEMGECFKGSTCPFLHGYPPE 141
[Close]
BLAST of Ccr4-not transcription complex, putative vs. Planmine SMEST
Match: SMESG000012074.1 (SMESG000012074.1)

HSP 1 Score: 1401.34 bits (3626), Expect = 0.000e+0
Identity = 740/746 (99.20%), Postives = 740/746 (99.20%), Query Frame = 2 
Query:  200 MDIIKEPIENILKVNYLSKNMELKMPNFIDSCLKVLENFTLMLLNTNEKISRFPKLNYCYCYFNAIRWQLINSASLNHNFIMEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICXXXXXXXXXXXXXXQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFTALTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITTDNSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTDSTVYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIXXXXXXXXXXXXXXXXXXXXXXXXXANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            MDI KEPIENILKVNYLSKNMEL MPNFIDSCLKVLENFTLMLLNTNEKISRFPKLNYCYCYFNAIRWQLINSASLNHNFIMEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICRRNNKRKKKSNKKRQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFT LTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITT NSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTD  VYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIQLNPQLQFSNPNNNNSSSDFSLISPANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE
Sbjct:    1 MDITKEPIENILKVNYLSKNMELNMPNFIDSCLKVLENFTLMLLNTNEKISRFPKLNYCYCYFNAIRWQLINSASLNHNFIMEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICRRNNKRKKKSNKKRQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFTTLTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITTVNSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTDLNVYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIQLNPQLQFSNPNNNNSSSDFSLISPANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 746
[Close]
BLAST of Ccr4-not transcription complex, putative vs. Planmine SMEST
Match: SMESG000012074.1 (SMESG000012074.1)

HSP 1 Score: 1242.64 bits (3214), Expect = 0.000e+0
Identity = 661/665 (99.40%), Postives = 661/665 (99.40%), Query Frame = 2 
Query:  443 MEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICXXXXXXXXXXXXXXQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFTALTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITTDNSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTDSTVYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIXXXXXXXXXXXXXXXXXXXXXXXXXANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 2437
            MEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICRRNNKRKKKSNKKRQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFT LTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITT NSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTD  VYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIQLNPQLQFSNPNNNNSSSDFSLISPANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE
Sbjct:    1 MEIAKSIIKRLQSSLRLFDTSKHCLSFSPESNKHRSILSPIIGKYNICRRNNKRKKKSNKKRQESLNRDNCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGIDQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDCKLSDDSHDIFSDQFDKLLALNNLDIDFTTLTAKLLTSNNGFMLPNKRFRKYSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKSDEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFNLNAEYNKQSEDKSSNFSKNSIEQITTVNSWPSRYPNILDNRMAYDEFSNIAPHNKSYETNELVTPDPNSLTSLVTDLNVYKDDRPDQKFHNASNIQQQSSATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNIDVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITPHLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIQLNPQLQFSNPNNNNSSSDFSLISPANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACCFYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE 665
The following BLAST results are available for this feature:
BLAST of Ccr4-not transcription complex, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3TFA22.066e-2068.97Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A3S4ZW844.356e-2070.49Uncharacterized protein OS=Protopolystoma xenopodi... [more]
A0A267EXZ11.129e-1963.49Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A183RJP46.191e-1961.43Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A183M2191.288e-1858.90Uncharacterized protein OS=Schistosoma margrebowie... [more]
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ccr4-not transcription complex, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000012074.10.000e+099.20SMESG000012074.1[more]
SMESG000012074.10.000e+099.40SMESG000012074.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025587 ID=SMED30025587|Name=Ccr4-not transcription complex, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=2897bp
CTCTTCCGATCTCAAATTCTTAAGGTTACTGTGGTCGAAGTCGCCATATA
GTCATCTGTAAAGAGATCATAATGGTAAAAATCTGTATATTTGAAAAGCA
TGCATGGTAACTTCTATTCCTGAGAGCATTTTGAAACTACATAAAAATTT
GAAAAAAATAATAATTTGTTGAATTAAATATCATTAAATCTTATCGTGAA
TGGATATTATAAAAGAGCCTATAGAAAATATTTTGAAAGTTAACTATCTA
TCAAAAAATATGGAATTAAAAATGCCTAATTTTATTGATAGCTGCTTAAA
AGTACTTGAAAATTTTACCCTGATGTTATTGAATACCAATGAAAAGATAA
GTCGATTCCCTAAATTAAATTATTGTTATTGTTATTTTAATGCAATTCGA
TGGCAACTTATTAATTCTGCCTCATTGAATCATAATTTTATCATGGAAAT
TGCAAAATCAATTATCAAACGTTTACAAAGTTCGCTGCGTCTTTTTGATA
CCTCAAAACATTGTCTTAGTTTCTCTCCTGAAAGTAACAAACATCGATCT
ATATTGAGTCCTATTATCGGTAAATATAACATTTGTCGACGCAACAATAA
AAGAAAAAAGAAATCAAATAAAAAAAGACAAGAATCATTAAATCGAGACA
ATTGTCAGAAAATAATTTTAAACAAATTCTATTGGAGTTTATTTTATTTG
AATAATGAAAATGCGAGTAACCTCAAAGCTTGTAACATGCAGCACATCAT
AGATCAAATGTATATTCAAATTCGCACAGTTCTTTCTGAGAATGGAATTG
ATCAAGAAGATATATTTAATGAATTGAATATATCAAAGGAGAGAGGCATT
TTATCAAAATCCATAAATAACAGAAAGCCATTGTCACCGCCAATTAATAC
AGATAGGAAACCATTGAAATATCAAGAACAATTATGGTCAGGAGATTGCA
AGCTTTCTGATGATAGCCATGATATTTTTTCCGATCAATTTGATAAGCTT
CTGGCACTCAATAACTTAGATATTGATTTCACTGCGTTAACTGCGAAACT
ATTGACGTCCAATAATGGATTTATGTTGCCCAACAAGCGCTTTCGGAAAT
ACTCAGAATTGGATGAAGGATTTTCAGAAACTGAATTTGTGTACGACTTA
AGCACAAAACAAGATGAAAATGAATATTCCGAATTTTTAAGCTGTGAGAA
ATTCTCATATTGCGGCGATTGCCATTATAATTCTAGTAATAGCAAATCCG
ACGAGAAAAATATCAACGACAAATGTTTAGAAAAAGATGAAACTCCAGTT
AAATTCATTGATGACGGTTCAGATAGTGATGGGGGGACGGAATTGCTTAA
TAGCGTGAATGACACAGTCAATAAATTCAATGCCTTTATGCAGTTCAATC
TTAATGCAGAGTATAACAAGCAATCAGAAGATAAGTCTTCTAATTTCTCC
AAAAACAGTATTGAGCAAATTACAACTGACAACTCTTGGCCGTCTCGCTA
TCCAAATATATTAGACAACAGAATGGCTTATGACGAATTTTCCAATATTG
CACCGCACAATAAATCTTATGAAACGAACGAATTAGTAACTCCAGATCCT
AATTCTTTGACATCCTTGGTTACAGATTCAACCGTTTACAAGGACGACCG
ACCCGATCAAAAGTTTCACAATGCAAGCAATATTCAACAACAGTCCTCCG
CGACTAGTCAATCAAATTTTAATTATGATACTCTATCCAAAATGGTAGTT
TCGCCTGTCAGTATTCAGAATTGGTGTTCATATAACTTTTCGCAAATACC
ATCGCAACCTTGCAATTTTGGTTACCAGACAAAGAGCAATTTGAATATAG
ATGTTAGCAGTGCTCAGACAGAAAATTATTGTTCCACTAAATTGTCGAAT
TGGTTACCTCCAATATCTCACGGGTTGATTACTTCTAATTCATACTTTAT
TGGATCAAATAGTGCTCAGCAACCTTTCCATAGAATCATGATTACACCGC
ACCTATCGCTGCCACCGGGGAATTTGACACCAGTTCAACATAATATCATT
CATCTTCCACAACTTTTCCATCAACTTCCACATTCTCTGATGGATACAGA
ATATTTTGATCGTCGGCAAATTCAATTAAATCCTCAATTGCAATTCTCTA
ATCCGAACAATAACAATTCAAGCAGTGATTTTTCACTGATATCACCAGCC
AATTTGATTGCAAACTCAAATAGTTCAAATATGTCATTACGTCAACCACC
GATGTCAATTATCAAAACATCTCTCGATAAATCTCAGAGAGCTTGCTGTT
TTTATATGCGCGGTGGTTGTAAAAAAGAAGATTGTGAATATGCGCATGAT
TTAAGTAAGGTGACGTGCAAATTTTGGGAAAAGGGTGATTGCTTCAAGGC
TGACACTTGTCCTTTCAAGCACGGATATGTGAAAGAGTAATTTCCCTCCT
TTTGATTTTCGGTTTTATTTTTTAAGATGTTTTTTTACTTTTTCGATAAG
TTATTCATTTTCGAATTTTGTCTTAAATTTTGCTCTTTCATTTATATTTT
CTGGATTGTTAGATATATTATTATTATCAGTCGTTATTATTTTCTCAAGA
TGTGCATTAATTTTTTGCTATTATTTTTTAAAATTGATTTTATATTTTTA
ATAAACTCGACAAGTCTTATATATATATATATTGAGCTATTCTATTATTC
TCACCATCTCCGTATCTGTCGCCTGTCTGTGTCTCTGTATTGACAATAAT
TGCTCTCCGTTTCATGGACTGAAATTTTTGATAAAGATGTTTGTTTCTTG
ATTCTTTGAGCTGAGCAAAAAAGTTGTTGAAAAAATAATCACATAAATAT
TTTCTTTTTTTCTCAATCATTCATTGAGGTTTCCTAATATATTCGGG
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protein sequence of SMED30025587-orf-1

>SMED30025587-orf-1 ID=SMED30025587-orf-1|Name=SMED30025587-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=747bp
MDIIKEPIENILKVNYLSKNMELKMPNFIDSCLKVLENFTLMLLNTNEKI
SRFPKLNYCYCYFNAIRWQLINSASLNHNFIMEIAKSIIKRLQSSLRLFD
TSKHCLSFSPESNKHRSILSPIIGKYNICRRNNKRKKKSNKKRQESLNRD
NCQKIILNKFYWSLFYLNNENASNLKACNMQHIIDQMYIQIRTVLSENGI
DQEDIFNELNISKERGILSKSINNRKPLSPPINTDRKPLKYQEQLWSGDC
KLSDDSHDIFSDQFDKLLALNNLDIDFTALTAKLLTSNNGFMLPNKRFRK
YSELDEGFSETEFVYDLSTKQDENEYSEFLSCEKFSYCGDCHYNSSNSKS
DEKNINDKCLEKDETPVKFIDDGSDSDGGTELLNSVNDTVNKFNAFMQFN
LNAEYNKQSEDKSSNFSKNSIEQITTDNSWPSRYPNILDNRMAYDEFSNI
APHNKSYETNELVTPDPNSLTSLVTDSTVYKDDRPDQKFHNASNIQQQSS
ATSQSNFNYDTLSKMVVSPVSIQNWCSYNFSQIPSQPCNFGYQTKSNLNI
DVSSAQTENYCSTKLSNWLPPISHGLITSNSYFIGSNSAQQPFHRIMITP
HLSLPPGNLTPVQHNIIHLPQLFHQLPHSLMDTEYFDRRQIQLNPQLQFS
NPNNNNSSSDFSLISPANLIANSNSSNMSLRQPPMSIIKTSLDKSQRACC
FYMRGGCKKEDCEYAHDLSKVTCKFWEKGDCFKADTCPFKHGYVKE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000571Znf_CCCH
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 694..718
e-value: 0.24
score: 20.5
coord: 719..743
e-value: 0.072
score: 22.2
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 717..744
score: 13.103
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 693..716
score: 8.409
NoneNo IPR availableGENE3DG3DSA:4.10.1000.10coord: 686..746
e-value: 8.9E-10
score: 40.4