SMED30025561

Overview
NameSMED30025561
Smed IDSMED30025561
Length (bp)2034
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025561 (SMED30025561) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025561 (SMED30025561) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025561 (SMED30025561) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025561 (SMED30025561) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30025561

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025561h1SMcG0004156 dd_Smed_v4_4282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30025561h1SMcG0004156 dd_Smed_v6_4282_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025561h1SMcG0004156 dd_Smed_v6_4282_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025561 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004417:19233..25689 -8210
Homology
BLAST of SMED30025561 vs. Planmine SMEST
Match: SMESG000023796.1 (SMESG000023796.1)

HSP 1 Score: 850.507 bits (2196), Expect = 0.000e+0
Identity = 551/581 (94.84%), Postives = 553/581 (95.18%), Query Frame = 3
Query:   90 MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIXXXXXXXXNLSDSRPGLMPYNNDFRPNINHDPRFPMXXXXXXXXXXXXVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWXXXXXXXXXXXXXXQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRXXXXXXXXXXXXXXXXXXXXXXXKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNSDATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGERHNRSPDNSHRXXXXXXXXXXXXXXXXXXXXXXPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 1832
            MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHD RFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFN+VPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWIIDDMKKIKEKEKKQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT                           LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSP+NSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS
Sbjct:    1 MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDARFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNSVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWIIDDMKKIKEKEKKQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT---------------------------LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSPNNSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 554          
BLAST of SMED30025561 vs. Planmine SMEST
Match: SMESG000022519.1 (SMESG000022519.1)

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 534/585 (91.28%), Postives = 537/585 (91.79%), Query Frame = 3
Query:   78 KKFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIXXXXXXXXNLSDSRPGLMPYNNDFRPNINHDPRFPMXXXXXXXXXXXXVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWXXXXXXXXXXXXXXQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRXXXXXXXXXXXXXXXXXXXXXXXKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNSDATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGERHNRSPDNSHRXXXXXXXXXXXXXXXXXXXXXXPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 1832
            +KFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHD RFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFN+VPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTR  K                  KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT                           LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSP+NSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS
Sbjct:  120 RKFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDARFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNSVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRKRK------------------KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT---------------------------LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSPNNSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 659          
BLAST of SMED30025561 vs. Planmine SMEST
Match: SMESG000022519.1 (SMESG000022519.1)

HSP 1 Score: 838.565 bits (2165), Expect = 0.000e+0
Identity = 534/585 (91.28%), Postives = 537/585 (91.79%), Query Frame = 3
Query:   78 KKFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIXXXXXXXXNLSDSRPGLMPYNNDFRPNINHDPRFPMXXXXXXXXXXXXVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWXXXXXXXXXXXXXXQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRXXXXXXXXXXXXXXXXXXXXXXXKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNSDATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGERHNRSPDNSHRXXXXXXXXXXXXXXXXXXXXXXPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 1832
            +KFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHD RFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFN+VPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTR  K                  KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT                           LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSP+NSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS
Sbjct:  115 RKFLMNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDARFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNSVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRKRK------------------KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT---------------------------LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSPNNSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 654          
BLAST of SMED30025561 vs. Planmine SMEST
Match: SMESG000022519.1 (SMESG000022519.1)

HSP 1 Score: 827.78 bits (2137), Expect = 0.000e+0
Identity = 531/581 (91.39%), Postives = 533/581 (91.74%), Query Frame = 3
Query:   90 MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIXXXXXXXXNLSDSRPGLMPYNNDFRPNINHDPRFPMXXXXXXXXXXXXVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWXXXXXXXXXXXXXXQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRXXXXXXXXXXXXXXXXXXXXXXXKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNSDATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGERHNRSPDNSHRXXXXXXXXXXXXXXXXXXXXXXPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 1832
            MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHD RFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFN+VPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTR  K                  KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT                           LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSP+NSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS
Sbjct:    1 MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDARFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNSVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRKRK------------------KQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT---------------------------LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSPNNSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 536          
BLAST of SMED30025561 vs. Planmine SMEST
Match: SMESG000023796.1 (SMESG000023796.1)

HSP 1 Score: 821.617 bits (2121), Expect = 0.000e+0
Identity = 538/568 (94.72%), Postives = 540/568 (95.07%), Query Frame = 3
Query:  129 MLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIXXXXXXXXNLSDSRPGLMPYNNDFRPNINHDPRFPMXXXXXXXXXXXXVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWXXXXXXXXXXXXXXQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRXXXXXXXXXXXXXXXXXXXXXXXKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNSDATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGERHNRSPDNSHRXXXXXXXXXXXXXXXXXXXXXXPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 1832
            MLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHD RFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFN+VPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWIIDDMKKIKEKEKKQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT                           LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSP+NSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS
Sbjct:    1 MLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHNIPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDARFPMNRNTQNFNINTNVYEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNSVPSYNRPPSTPSFSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPSMPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWIIDDMKKIKEKEKKQPMTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDSLITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIRDSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVT---------------------------LDAYESDDEVQIVEDKSEATGKKTVVSEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSKNKHKSRSRDHSKKSKSGERHNRSPNNSHRSRSKNKNDSWSSSNKKTKKHNSPDWRDSKWKNDKCESNNSSKKHNKHDRSRS 541          
The following BLAST results are available for this feature:
BLAST of SMED30025561 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025561 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023796.10.000e+094.84SMESG000023796.1[more]
SMESG000022519.10.000e+091.28SMESG000022519.1[more]
SMESG000022519.10.000e+091.28SMESG000022519.1[more]
SMESG000022519.10.000e+091.39SMESG000022519.1[more]
SMESG000023796.10.000e+094.72SMESG000023796.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025561 ID=SMED30025561|Name=SMED30025561|organism=Schmidtea mediterranea sexual|type=transcript|length=2034bp
GAAATAAATCACTTTATAAAGATTTGTTTAAATTATTCAAATAATATATT
TGTATTTAAACATATTTCCATTTGTTGAAAAAATTCCTAATGAACCATCA
AATTCCTCAATATAATCAAAATATTCCGATGTTAAGCCACACTGATTGGG
CATCTATGGCTAAACAATGGGCTACGTTACGGGCTAACGTAAACATACCA
TCATCTATAAATAAAGTTGAAAGTTACACCTATCATAATATACCACCCCC
ACCACCACCTCTTCCTAATTTGTCAGATTCAAGACCAGGTCTGATGCCAT
ACAATAATGATTTTAGACCAAATATAAATCATGACCCAAGATTTCCTATG
AATCGAAATACTCAAAATTTTAACATAAATACTAATGTTTATGAGCATCC
AATGCATTCGATATATGAAGAAGTAAATTCATCAAATAATAATTCTCAGT
GTGAGACGTTTGATTACAATCATCGTGATAGGCCGTTTTTAAATTTCAAT
AATGTTCCTTCTTATAACCGGCCTCCTTCAACACCGTCTTTTTCACAAAA
TAATTTTAATTTGCCCCCACCAACTCATCAAGATTTTGTTGAAACTCAAC
CAATCAGGGCTCATACATTTCAAGATCCAACTCCACCGAAATTGTTTCCC
ATTGATGTGAAAAGTCAACCTATATTGCAACCTCCATCTATGCCTATTAA
AATTGAACAACCGCCACCATTCATGATTAGCAAATTTGATAATCAAACGG
ATTTTTCTGGAACCAGGTCAACTAAAGGCTTACCAGCGTGGATAATTGAT
GATATGAAAAAAATTAAAGAAAAAGAAAAAAAACAACCCATGACATCTAT
AGATGATTATAGAGATCATTATATTAACTCAAAACGCGATGAAGAAGGGG
ACATAGTAATGGAAGACGTCAATAAGGAATCGGATTTCGATCCTATTTTG
TCAGGTAAAAGTGTTATTACAACAAATCGAATAGATAGTCTGATCACGGA
CATTGATATTGATGATGTCAATCTCAACGATGAAGATGACGGTTTCGATA
AAATTGATTATTCATCTAAATCTTTAATTGATTTGATTGAGATGCTCGAT
GAAAATGAGCTCGGGGCTCTGAAGATGGTTATAATCCGTGATTCCTTGAC
TGGCGTTATGAAAGAAGTTACAGATGACATGATAACTGAAGCTGTGAGTT
TCACATTTTCCAGTGAACTGTCTAAAATTAGTGTCAAATCCAAGCCTAAA
AACGTTTCAATGGATTCTAGGACTGTTACTTGCAATAGTGATGCAACTGT
TAAACCAATAGATCGTCCAATGGCGGAATTTCAAAATGAATGCACATCCC
GTCTACTCGGACTTGACGCTTATGAATCAGACGATGAAGTTCAAATCGTT
GAAGATAAATCAGAAGCAACTGGAAAAAAGACTGTTGTATCCGAAGCTCG
AGATAAATGGTCTACTCATAAAAAGTCATTGCCAAAAGTAGATCAAAACT
CATCACGTAGTAAATTAGAAGGAAAAGATGAATCAAAAGACATAAAAAAA
TCCTCATCCGATACTAACAGTAGTCGAAATTCTTCGAAAAACAAACATAA
ATCACGATCACGTGACCATAGTAAAAAGTCGAAGTCCGGTGAACGGCACA
ATCGCTCACCCGATAATAGCCATCGATCGCGATCCAAAAATAAAAATGAC
TCGTGGTCTTCATCAAATAAAAAGACCAAAAAACATAATTCTCCAGATTG
GAGAGATAGTAAATGGAAGAATGATAAATGTGAATCTAATAACAGCAGTA
AAAAACACAATAAACATGACAGAAGTAGGTCCTAACTTTGATTTGTTGAA
AATTGCGCGTTCGAAATCAATGGTAATTTCCTTATTAACAGAAAATACGG
TAAGTACTTTTGAAATTTTTTGTCATATTCAATCATAGTAAAAGCTCTTT
TTTCATGAATATTTGCACTATAGTCTGTCAGTTATGTATTTATAACTAGC
TTTATTATGACAAAAAAATTTAATTATTTTGGTA
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protein sequence of SMED30025561-orf-1

>SMED30025561-orf-1 ID=SMED30025561-orf-1|Name=SMED30025561-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=582bp
MNHQIPQYNQNIPMLSHTDWASMAKQWATLRANVNIPSSINKVESYTYHN
IPPPPPPLPNLSDSRPGLMPYNNDFRPNINHDPRFPMNRNTQNFNINTNV
YEHPMHSIYEEVNSSNNNSQCETFDYNHRDRPFLNFNNVPSYNRPPSTPS
FSQNNFNLPPPTHQDFVETQPIRAHTFQDPTPPKLFPIDVKSQPILQPPS
MPIKIEQPPPFMISKFDNQTDFSGTRSTKGLPAWIIDDMKKIKEKEKKQP
MTSIDDYRDHYINSKRDEEGDIVMEDVNKESDFDPILSGKSVITTNRIDS
LITDIDIDDVNLNDEDDGFDKIDYSSKSLIDLIEMLDENELGALKMVIIR
DSLTGVMKEVTDDMITEAVSFTFSSELSKISVKSKPKNVSMDSRTVTCNS
DATVKPIDRPMAEFQNECTSRLLGLDAYESDDEVQIVEDKSEATGKKTVV
SEARDKWSTHKKSLPKVDQNSSRSKLEGKDESKDIKKSSSDTNSSRNSSK
NKHKSRSRDHSKKSKSGERHNRSPDNSHRSRSKNKNDSWSSSNKKTKKHN
SPDWRDSKWKNDKCESNNSSKKHNKHDRSRS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 511..572
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 438..581
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 438..490