Leucine-rich repeat-containing protein 71

Overview
NameLeucine-rich repeat-containing protein 71
Smed IDSMED30025542
Length (bp)751
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Leucine-rich repeat-containing protein 71 (SMED30025542) t-SNE clustered cells

Violin plots show distribution of expression levels for Leucine-rich repeat-containing protein 71 (SMED30025542) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025542

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30025542h1SMcG0007921 SmedASXL_016179SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30025542h1SMcG0007921 dd_Smed_v4_10715_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025542h1SMcG0007921 dd_Smed_v4_10715_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025542h1SMcG0007921 Contig42074newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025542h1SMcG0007921 Contig42074uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30025542h1SMcG0007921 dd_Smed_v4_12043_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025542h1SMcG0007921 dd_Smed_v4_12043_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025542h1SMcG0007921 dd_Smed_v4_12043_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025542h1SMcG0007921 dd_Smed_v4_12043_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30025542h1SMcG0007921 dd_Smed_v6_10715_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025542h1SMcG0007921 dd_Smed_v6_12043_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30025542h1SMcG0007921 dd_Smed_v4_12043_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Human
Match: LRRC71 (leucine rich repeat containing 71 [Source:HGNC Symbol;Acc:HGNC:26556])

HSP 1 Score: 67.0106 bits (162), Expect = 3.722e-12
Identity = 58/205 (28.29%), Postives = 88/205 (42.93%), Query Frame = 3 
Query:   57 SFKAMGKKTEKAIKEKPT--VQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCET 665
            S  A+ KK E+A KEKP   +    E   K+PE YQC G   TDF EL            C R   G+T               K  ++    P  V     SE+ + +D  +  S         ++  +   +P + VE +  D   +V EI +R W V+  ++ +F  C   LT+L  I+ W+VGLT + ++   E L  C +
Sbjct:   22 SSGAVTKKGERAAKEKPATVLPPVGEEEPKSPEEYQCSGVLETDFAEL------------CTR--WGYT------------DFPKVVNRPRPHPPFVPSASLSEKATLDDPRLSGSCSLN----SLESKYVFFRPTIQVELEQEDS-KSVKEIYIRGWKVEERILGVFSKCLPPLTQLQAINLWKVGLTDKTLTTFIELLPLCSS 195
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Zebrafish
Match: si:ch211-81a5.5 (si:ch211-81a5.5 [Source:ZFIN;Acc:ZDB-GENE-060503-892])

HSP 1 Score: 48.521 bits (114), Expect = 3.138e-6
Identity = 37/145 (25.52%), Postives = 64/145 (44.14%), Query Frame = 3 
Query:  300 GKKGSKDDKRPQVVQHQPD---------SEQLSQEDKEVVNSIGFK--PRTFVIKPTFCALK-----PALC--VETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
             +K SK DK    ++ +P          S  L  E  E+   +G K  P   V +P     +     P  C  +E +N +  +NV E+ +  W VD  ++++      SL+ L  +  WR+GLT    + +  T+S C  ++ V+
Sbjct:    5 AEKPSKVDKVDSAIEEEPKPKTADDYQCSGHLQLEFPELCKLMGVKEIPSVKVKRPRILESRMTKWSPGRCLQIELENEEH-NNVKEVKISGWTVDELMLQVLSKILPSLSNLLCLRMWRIGLTDGTFTSMKNTVSLCSNLRKVV 148
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Xenopus
Match: emp3 (epithelial membrane protein 3 [Source:Xenbase;Acc:XB-GENE-952486])

HSP 1 Score: 68.5514 bits (166), Expect = 1.053e-12
Identity = 65/208 (31.25%), Postives = 87/208 (41.83%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQA----GKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
            MGKK+EKA K+K      E+    + + YQC G    DF EL            C R   GFT + +   +  +  A     +K + D           DS+  S +D    N  G  P     K  F   KP + VET+  D   +V  I V  W +D  ++ +F  C  +LT L  IS W V LT    SQ  E L  C  IK V+
Sbjct:    1 MGKKSEKAQKDKVAAGNAEDESRNSGD-YQCTGILEQDFPEL------------CGR--AGFTESEIPRVTLCHRTAVTPTSEKNAAD-----------DSDGASDKD----NPGGGGP----TKDKFSYFKPKIEVETEGEDG-KSVKVIFVHGWKIDHRMMGIFSKCLPALTALQGISLWNVALTESTFSQFLEILPHCPNIKTVV 173
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Xenopus
Match: emp3 (epithelial membrane protein 3 [Source:Xenbase;Acc:XB-GENE-952486])

HSP 1 Score: 65.0846 bits (157), Expect = 1.538e-11
Identity = 67/219 (30.59%), Postives = 87/219 (39.73%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTTE-----------EIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQA----GKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
            MGKK+EKA K+K      E           E  + T   YQC G    DF EL            C R   GFT + +   +  +  A     +K + D           DS+  S +D    N  G  P     K  F   KP + VET+  D   +V  I V  W +D  ++ +F  C  +LT L  IS W V LT    SQ  E L  C  IK V+
Sbjct:    1 MGKKSEKAQKDKVAAGNAEDESRNSANASMERASLTVGDYQCTGILEQDFPEL------------CGR--AGFTESEIPRVTLCHRTAVTPTSEKNAAD-----------DSDGASDKD----NPGGGGP----TKDKFSYFKPKIEVETEGEDG-KSVKVIFVHGWKIDHRMMGIFSKCLPALTALQGISLWNVALTESTFSQFLEILPHCPNIKTVV 185
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Mouse
Match: Lrrc71 (leucine rich repeat containing 71 [Source:MGI Symbol;Acc:MGI:1921735])

HSP 1 Score: 63.1586 bits (152), Expect = 4.683e-11
Identity = 58/205 (28.29%), Postives = 85/205 (41.46%), Query Frame = 3 
Query:   57 SFKAMGKKTEKAIKEK--PTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCET 665
            S  A+ KK ++A K+K   T+    E   K PE YQC G   TDF EL      GY     +   K  T   +   S  +    +K   DD+RP          Q S E K                  +   +P + VE +  D    V EI +R W V+  ++ +F  C  SL++L  I+ W+VGLT + ++     L  C +
Sbjct:   22 SSGAVTKKGDRAAKDKTASTLPPVGEDEPKNPEEYQCTGVLETDFAELCTRS--GY-----VDFPKVVTRPRVQQSSVPSASTSEKPVLDDQRPSA-----SCSQSSLESK------------------YVFFRPTIQVELEQEDS-KAVKEIYIRGWKVEDRILGIFSKCLPSLSQLQAINLWKVGLTDKTLTTFIALLPLCSS 195
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. UniProt/SwissProt
Match: sp|Q8N4P6|LRC71_HUMAN (Leucine-rich repeat-containing protein 71 OS=Homo sapiens OX=9606 GN=LRRC71 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.787e-11
Identity = 58/205 (28.29%), Postives = 88/205 (42.93%), Query Frame = 3 
Query:   57 SFKAMGKKTEKAIKEKPT--VQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCET 665
            S  A+ KK E+A KEKP   +    E   K+PE YQC G   TDF EL            C R   G+T               K  ++    P  V     SE+ + +D  +  S         ++  +   +P + VE +  D   +V EI +R W V+  ++ +F  C   LT+L  I+ W+VGLT + ++   E L  C +
Sbjct:   22 SSGAVTKKGERAAKEKPATVLPPVGEEEPKSPEEYQCSGVLETDFAEL------------CTR--WGYT------------DFPKVVNRPRPHPPFVPSASLSEKATLDDPRLSGSCSLN----SLESKYVFFRPTIQVELEQEDS-KSVKEIYIRGWKVEERILGVFSKCLPPLTQLQAINLWKVGLTDKTLTTFIELLPLCSS 195
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. UniProt/SwissProt
Match: sp|Q9D3W5|LRC71_MOUSE (Leucine-rich repeat-containing protein 71 OS=Mus musculus OX=10090 GN=Lrrc71 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 3.276e-10
Identity = 58/205 (28.29%), Postives = 85/205 (41.46%), Query Frame = 3 
Query:   57 SFKAMGKKTEKAIKEK--PTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCET 665
            S  A+ KK ++A K+K   T+    E   K PE YQC G   TDF EL      GY     +   K  T   +   S  +    +K   DD+RP          Q S E K                  +   +P + VE +  D    V EI +R W V+  ++ +F  C  SL++L  I+ W+VGLT + ++     L  C +
Sbjct:   22 SSGAVTKKGDRAAKDKTASTLPPVGEDEPKNPEEYQCTGVLETDFAELCTRS--GY-----VDFPKVVTRPRVQQSSVPSASTSEKPVLDDQRPSA-----SCSQSSLESK------------------YVFFRPTIQVELEQEDS-KAVKEIYIRGWKVEDRILGIFSKCLPSLSQLQAINLWKVGLTDKTLTTFIALLPLCSS 195
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Match: A0A0B7BMA1 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF195341 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 3.471e-30
Identity = 80/210 (38.10%), Postives = 118/210 (56.19%), Query Frame = 3 
Query:   69 MGKKTEKAI-KEK---PTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFK--PRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
            MGKK EK++ KE+   P  +T E  +NKT EPY+C GNF+ DFTEL +++   Y   V  R K     + + SDS  + +A KK     +   + + + DSE         +N   F+  P+T+ I+  F   KP++ VE DN DK D VTE+SV+ W VD  ++++F   +  L+KL T++FW VGLT E +  LA  L  C  +K +I
Sbjct:    1 MGKKVEKSVSKERQPNPAGRTEETDLNKTTEPYKCNGNFLVDFTELCKQRQMTYIPSVITRPKLQILQSIVTSDSKLDKKADKK-----QAAVISETEHDSE---------INEAVFEGPPKTYTIRDKFSFFKPSVQVELDNNDKWDTVTELSVKGWKVDEPMMDVFQHSWIHLSKLHTVNFWNVGLTEETLHTLAIFLPHCANVKNLI 196
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Match: A0A0B7BKQ4 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF195344 PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 6.050e-30
Identity = 80/210 (38.10%), Postives = 118/210 (56.19%), Query Frame = 3 
Query:   69 MGKKTEKAI-KEK---PTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFK--PRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
            MGKK EK++ KE+   P  +T E  +NKT EPY+C GNF+ DFTEL +++   Y   V  R K     + + SDS  + +A KK     +   + + + DSE         +N   F+  P+T+ I+  F   KP++ VE DN DK D VTE+SV+ W VD  ++++F   +  L+KL T++FW VGLT E +  LA  L  C  +K +I
Sbjct:    1 MGKKVEKSVSKERQPNPAGRTEETDLNKTTEPYKCNGNFLVDFTELCKQRQMTYIPSVITRPKLQILQSIVTSDSKLDKKADKK-----QAAVISETEHDSE---------INEAVFEGPPKTYTIRDKFSFFKPSVQVELDNNDKWDTVTELSVKGWKVDEPMMDVFQHSWIHLSKLHTVNFWNVGLTEETLHTLAIFLPHCANVKNLI 196
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Match: A0A2C9LPR1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106058773 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 8.397e-29
Identity = 73/201 (36.32%), Postives = 107/201 (53.23%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTT----EEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKC 659
            MGKK EK+  +   + +     +E  NKTPEP++C G+F  DFTEL + QN    +P  +  +K  T    + D         K SK + + Q V    D+ +   E  E    I   P+T+  KP F  LKP++ VE DN DK D VTE+ VR W +D++++E+F  C+ +L +L TI+ W  GLT   +  LA  L +C
Sbjct:    1 MGKKVEKSFSKDRHLSSAAHHDDENSNKTPEPHKCTGSFEKDFTELCK-QNAMSAIPNVLLRQKSLTGQSGSVDL--------KSSKREDKKQPVAQVHDASEADTEINEDGEPIELPPKTYTTKPIFEYLKPSVQVEMDNPDKFDTVTEVYVRGWKIDATMMEIFRQCWLTLDRLHTINLWNTGLTESTLHTLATFLPQC 192
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Match: A0A2C9LPG7 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106058773 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 8.698e-29
Identity = 73/201 (36.32%), Postives = 107/201 (53.23%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTT----EEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKC 659
            MGKK EK+  +   + +     +E  NKTPEP++C G+F  DFTEL + QN    +P  +  +K  T    + D         K SK + + Q V    D+ +   E  E    I   P+T+  KP F  LKP++ VE DN DK D VTE+ VR W +D++++E+F  C+ +L +L TI+ W  GLT   +  LA  L +C
Sbjct:   17 MGKKVEKSFSKDRHLSSAAHHDDENSNKTPEPHKCTGSFEKDFTELCK-QNAMSAIPNVLLRQKSLTGQSGSVDL--------KSSKREDKKQPVAQVHDASEADTEINEDGEPIELPPKTYTTKPIFEYLKPSVQVEMDNPDKFDTVTEVYVRGWKIDATMMEIFRQCWLTLDRLHTINLWNTGLTESTLHTLATFLPQC 208
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Match: A0A2R3SK17 (Leucine rich repeat only protein 9 OS=Argopecten irradians OX=31199 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.599e-28
Identity = 75/206 (36.41%), Postives = 108/206 (52.43%), Query Frame = 3 
Query:   69 MGKKTEKAIKEK--PTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLVI 680
            MGKK EK++KEK    V   E+  NKTPEPY C GNF  DFTEL +  N     PV  R +   + + + S      +   K S    + Q  + +PD + +  E  E        P+TFV+K  F   KP++ VE +N DKPD VTEI +R W +D +++  F  C+  + KL TI+ W  GLT E I+ +A  +     ++ +I
Sbjct:    1 MGKKVEKSLKEKNQSAVSQDEDDPNKTPEPYNCTGNFQADFTELCKRNNMAVIPPVVHRARPPTSQSQVESSKPEKGKEKGKTSA---QQQQAEPEPDPDMVEGEPVEP------PPKTFVLKEKFEYFKPSVQVEMENYDKPDTVTEIFIRGWKIDETMMATFAQCWPVMEKLHTINLWNTGLTGETITMMANCVPNIVNLRTLI 197
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Sea Lamprey
Match: si:ch211-81a5.5 (si:ch211-81a5.5 [Source:ZFIN;Acc:ZDB-GENE-060503-892])

HSP 1 Score: 83.1889 bits (204), Expect = 1.453e-18
Identity = 63/211 (29.86%), Postives = 89/211 (42.18%), Query Frame = 3 
Query:   75 KKTEKAIKEKPT--VQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIG-----FKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIK-LVIE 683
            KKTE+A +E       +TE+    TPE YQC GNF  DFT+L            C       T TGLAS                  P VV+       +     E V ++        P TF  K  F   KP + VE +N D   +V EI +R W ++  ++ +   C  ++  LTTI+FW  G+  + +      L  C  +K LV+E
Sbjct:    1 KKTERAARESKGQLSNSTEDDSINTPEEYQCNGNFEQDFTQL------------C-------TLTGLAST-----------------PAVVRRSHPPTAMENTGAENVQNVAPSEEDITPTTFTTKDKFVYFKPCVQVEPENEDNRSSVKEIYIRGWKIEEKMMSILKKCLPAIPSLTTINFWNAGIDEDTLDMFVSVLPLCTNLKTLVLE 175
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Planmine SMEST
Match: SMESG000006778.1 (SMESG000006778.1)

HSP 1 Score: 421.779 bits (1083), Expect = 1.050e-146
Identity = 207/227 (91.19%), Postives = 210/227 (92.51%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIK-LVIECK*FTRRHDCQ*SERFHRKVK 746
            MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDN DKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIK L IE       +    SE +H  +K
Sbjct:    1 MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNPDKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKFLTIE------NNQEASSENYHELIK 221
[Close]
BLAST of Leucine-rich repeat-containing protein 71 vs. Planmine SMEST
Match: SMESG000006778.1 (SMESG000006778.1)

HSP 1 Score: 413.69 bits (1062), Expect = 1.339e-143
Identity = 200/211 (94.79%), Postives = 201/211 (95.26%), Query Frame = 3 
Query:   69 MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDK--------EVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKLV 677
            MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDK        EVVNSIGFKPRTFVIKPTFCALKPALCVETDN DKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIK +
Sbjct:    1 MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGYQLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQLSQEDKDSIDDFYPEVVNSIGFKPRTFVIKPTFCALKPALCVETDNPDKPDNVTEISVRRWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETIKFL 211
The following BLAST results are available for this feature:
BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
LRRC713.722e-1228.29leucine rich repeat containing 71 [Source:HGNC Sym... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:ch211-81a5.53.138e-625.52si:ch211-81a5.5 [Source:ZFIN;Acc:ZDB-GENE-060503-8... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
emp31.053e-1231.25epithelial membrane protein 3 [Source:Xenbase;Acc:... [more]
emp31.538e-1130.59epithelial membrane protein 3 [Source:Xenbase;Acc:... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Lrrc714.683e-1128.29leucine rich repeat containing 71 [Source:MGI Symb... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q8N4P6|LRC71_HUMAN1.787e-1128.29Leucine-rich repeat-containing protein 71 OS=Homo ... [more]
sp|Q9D3W5|LRC71_MOUSE3.276e-1028.29Leucine-rich repeat-containing protein 71 OS=Mus m... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B7BMA13.471e-3038.10Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
A0A0B7BKQ46.050e-3038.10Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A2C9LPR18.397e-2936.32Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9LPG78.698e-2936.32Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2R3SK171.599e-2836.41Leucine rich repeat only protein 9 OS=Argopecten i... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:ch211-81a5.51.453e-1829.86si:ch211-81a5.5 [Source:ZFIN;Acc:ZDB-GENE-060503-8... [more]
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Leucine-rich repeat-containing protein 71 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000006778.11.050e-14691.19SMESG000006778.1[more]
SMESG000006778.11.339e-14394.79SMESG000006778.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025542 ID=SMED30025542|Name=Leucine-rich repeat-containing protein 71|organism=Schmidtea mediterranea sexual|type=transcript|length=751bp
TTTAAAAATCCGGAGTCTTGGAAACATATGAAAATAACTTTTTAGTCATA
AAGTAATCTTTTAAAGCAATGGGCAAAAAAACAGAAAAGGCTATTAAAGA
AAAACCAACAGTACAAACAACAGAAGAAATTGTGAATAAAACTCCTGAAC
CCTATCAATGCGTTGGAAATTTTGTGACTGACTTCACCGAGTTATGGAGG
GAACAAAATCCAGGATACCAGTTACCAGTTTGTATAAGGGATAAAAAGGG
ATTTACTGCAACAGGGCTTGCCAGTGATTCAAATTCAAATCTACAAGCAG
GAAAGAAGGGAAGCAAAGACGATAAGCGACCTCAAGTAGTTCAACATCAA
CCAGATTCTGAACAATTATCACAGGAAGATAAAGAAGTTGTGAATTCCAT
AGGCTTTAAACCTCGAACATTTGTTATTAAACCAACCTTTTGTGCATTGA
AGCCTGCCTTATGTGTTGAAACGGACAATGCAGATAAGCCTGACAACGTT
ACCGAAATTTCTGTAAGAAGATGGATGGTGGATTCTTCAGTTATTGAATT
ATTTGGCTTGTGCTTTTTTTCTCTCACAAAATTAACAACAATTTCATTTT
GGCGTGTTGGTCTTACTTCTGAAGCAATCTCCCAGCTTGCTGAGACATTA
TCCAAGTGTGAAACTATCAAGTTAGTGATAGAATGTAAATGATTTACCAG
ACGTCATGACTGTCAATGAAGTGAACGGTTTCATAGAAAGGTCAAACCGA
G
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protein sequence of SMED30025542-orf-1

>SMED30025542-orf-1 ID=SMED30025542-orf-1|Name=SMED30025542-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=208bp
MGKKTEKAIKEKPTVQTTEEIVNKTPEPYQCVGNFVTDFTELWREQNPGY
QLPVCIRDKKGFTATGLASDSNSNLQAGKKGSKDDKRPQVVQHQPDSEQL
SQEDKEVVNSIGFKPRTFVIKPTFCALKPALCVETDNADKPDNVTEISVR
RWMVDSSVIELFGLCFFSLTKLTTISFWRVGLTSEAISQLAETLSKCETI
KLVIECK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..103
NoneNo IPR availablePANTHERPTHR46984FAMILY NOT NAMEDcoord: 1..203