TNF receptor associated factor-2

Overview
NameTNF receptor associated factor-2
Smed IDSMED30025535
Length (bp)670
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TNF receptor associated factor-2 (SMED30025535) t-SNE clustered cells

Violin plots show distribution of expression levels for TNF receptor associated factor-2 (SMED30025535) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TNF receptor associated factor-2 (SMED30025535) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TNF receptor associated factor-2 (SMED30025535) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025535

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025535SMESG000049696.1 SMESG000049649.1 SMESG000049621.1 SMESG000049603.1 SMESG000049545.1 SMESG000017435.1 SMESG000072318.1 SMESG000058718.1 SMESG000048483.1 SMESG000017533.1 SMESG000016079.1 SMESG000014105.1 SMESG000006480.1 SMESG000005275.1 SMESG000001086.1 Contig42692newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025535SMESG000049696.1 SMESG000049649.1 SMESG000049621.1 SMESG000049603.1 SMESG000049545.1 SMESG000017435.1 SMESG000072318.1 SMESG000058718.1 SMESG000048483.1 SMESG000017533.1 SMESG000016079.1 SMESG000014105.1 SMESG000006480.1 SMESG000005275.1 SMESG000001086.1 Contig42692uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30025535SMESG000049696.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000049545.1 SMESG000049503.1 dd_Smed_v4_3528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30025535SMESG000049696.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000049545.1 SMESG000049503.1 dd_Smed_v4_3528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30025535SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: I1ZID8 (TNF receptor associated factor-2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.276e-23
Identity = 52/143 (36.36%), Postives = 87/143 (60.84%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQI 430
            Y+   + +CPG ++ C+ + +    N+  D  VN KI+K  VKCP + C++  EL+ +EEH+R C  +S +CPF  +GC   ++  +++  HL    + HSKLIID+I+N RN+   L  + +ELR ++  L K + QS+ +I
Sbjct:   54 YVSRGNLYCPGGTSECKEENLTICSNIHTDYRVNRKIAKMRVKCPEETCKVYCELVDIEEHLRLCTMKSQNCPFFVLGCNQPKMVIQDLTNHLQNGTFQHSKLIIDIISNMRNDFFGLTTKEVELREDIN-LIKNHFQSQNEI 195
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: A0A0F7GWW3 (TNF receptor associated factor 4 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 8.213e-20
Identity = 52/142 (36.62%), Postives = 85/142 (59.86%), Query Frame = 2 
Query:   68 FTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENME-------LRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKM 472
              +N+  D  +N+KI+K  VKC  + C+   EL  ME+H RTCK RS++CP+ +IGC+   I+ +++  HL    + H+KL++D + NF NEME LK  N         LRNE+ ELK    + + +I +++ +  NQ +++
Sbjct:   12 LNENICVDYPMNMKIAKLQVKCKEKSCDYVDELRKMEDHSRTCKYRSVACPYSNIGCDKCNISQDKVTDHLQSEIFVHTKLLMDFVVNFNNEMEMLKANNNGLSPVNELLRNEINELKLDGDRRRVEIGDLQRKCANQAEEI 153
[Close]
BLAST of TNF receptor associated factor-2 vs. TrEMBL
Match: T1E101 (TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 9.196e-14
Identity = 37/115 (32.17%), Postives = 67/115 (58.26%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEM 346
            YL ++  +CPGD   C+   +N   +V  +  ++ +IS+ +VKCP  +C   +EL+ ++EH+  C   S+ CP ++IGCE   +  +EM  HL +    H++LI+   N  ++E+
Sbjct:   58 YLSQNHNYCPGDKTECKEDYLNLEGDVFRNIRLSTRISRIVVKCPNIRCNYTSELLRLKEHLDKCTPESVECPCVTIGCEEEPLLKDEMAKHLTDQVLRHTQLIMSFCNELKSEI 172
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049567.1 (SMESG000049567.1)

HSP 1 Score: 329.331 bits (843), Expect = 1.037e-111
Identity = 197/223 (88.34%), Postives = 209/223 (93.72%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 670
            YL+E DKFCPG++ +CQ + INFT+NV+ED   NI+IS+ IVKCP Q CE +TEL MMEEH+RTC KR ISCPFISIGCEINEIANEE+NAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM
Sbjct:   46 YLNETDKFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFISIGCEINEIANEEINAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 268

HSP 2 Score: 58.9214 bits (141), Expect = 5.155e-10
Identity = 31/51 (60.78%), Postives = 41/51 (80.39%), Query Frame = 2 
Query:  341 EMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQ 493
            E+ ++K EN  LR EMTEL+++NR+ KQQIDNI+  STNQ QKM EMEA++
Sbjct:  222 EIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEMEARE 272
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049567.1 (SMESG000049567.1)

HSP 1 Score: 329.331 bits (843), Expect = 1.145e-111
Identity = 197/223 (88.34%), Postives = 209/223 (93.72%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 670
            YL+E DKFCPG++ +CQ + INFT+NV+ED   NI+IS+ IVKCP Q CE +TEL MMEEH+RTC KR ISCPFISIGCEINEIANEE+NAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM
Sbjct:   53 YLNETDKFCPGEAKYCQVQKINFTRNVSEDHPTNIRISEVIVKCPTQNCETKTELSMMEEHIRTCDKRPISCPFISIGCEINEIANEEINAHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 275

HSP 2 Score: 58.9214 bits (141), Expect = 4.676e-10
Identity = 45/94 (47.87%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:  341 EMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQID 622
            E+ ++K EN  LR EMTEL+++NR+ KQQIDNI+  STNQ QKM EMEA++ ENA EI              E L EEM  L+ E R    Q++
Sbjct:  229 EIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEMEAREQENAGEI--------------ELLNEEMVVLKNEYRSMSSQME 308
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049614.1 (SMESG000049614.1)

HSP 1 Score: 323.553 bits (828), Expect = 1.482e-110
Identity = 194/223 (87.00%), Postives = 204/223 (91.48%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 670
            YL E DKFCPG++ +CQ + INFT+NV+ED   N++IS+ IVKCP Q CE +TEL MMEEHMRTC KR ISCPF SIGCEINEIANEEMNAHLNESNYWHSKLIIDL NNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLEN EEIKKINKEIVAVKKENESLREEMTELRRENREFKQQ DNIQSLSTNQQQKMGEM
Sbjct:   46 YLKETDKFCPGEAKYCQVQKINFTRNVSEDHPANLRISEVIVKCPTQNCETKTELGMMEEHMRTCDKRPISCPFNSIGCEINEIANEEMNAHLNESNYWHSKLIIDLFNNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLENTEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQFDNIQSLSTNQQQKMGEM 268

HSP 2 Score: 56.6102 bits (135), Expect = 2.825e-9
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 2 
Query:  323 INNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQ 493
            I     E+ ++K EN  LR EMTEL+++NR+ KQQ DNI+  STNQ QKM EMEA++
Sbjct:  216 IKKINKEIVAVKKENESLREEMTELRRENREFKQQFDNIQSLSTNQQQKMGEMEARE 272
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049688.1 (SMESG000049688.1)

HSP 1 Score: 323.553 bits (828), Expect = 2.461e-109
Identity = 193/223 (86.55%), Postives = 207/223 (92.83%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 670
            YL+++DKFCPG++ +CQ + INFT+NV+ED   N++IS+ IVKCPRQ CE +TEL MMEEHMRTC KR ISCPF SIGCE+NEIANEEMNAHLNESNYWHSKLIIDL NNFRNEMESLKN NMELRNEMTELKKKNRQSK QIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM
Sbjct:   46 YLNQNDKFCPGEAKYCQVQKINFTRNVSEDHTANLRISEVIVKCPRQNCETKTELSMMEEHMRTCDKRPISCPFNSIGCEMNEIANEEMNAHLNESNYWHSKLIIDLFNNFRNEMESLKNGNMELRNEMTELKKKNRQSKHQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 268

HSP 2 Score: 61.6178 bits (148), Expect = 5.675e-11
Identity = 46/94 (48.94%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:  341 EMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQID 622
            E+ ++K EN  LR EMTEL+++NR+ KQQIDNI+  STNQ QKM EMEA++ ENA EI              E L EEM  L+ ENR    Q++
Sbjct:  222 EIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEMEAREQENAGEI--------------ELLNEEMVVLKNENRSMSSQME 301
[Close]
BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Match: SMESG000049688.1 (SMESG000049688.1)

HSP 1 Score: 323.168 bits (827), Expect = 3.552e-109
Identity = 193/223 (86.55%), Postives = 207/223 (92.83%), Query Frame = 2 
Query:    2 YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCEIQTELIMMEEHMRTCKKRSISCPFISIGCXXXXXXXXXXXXHLNESNYWHSKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 670
            YL+++DKFCPG++ +CQ + INFT+NV+ED   N++IS+ IVKCPRQ CE +TEL MMEEHMRTC KR ISCPF SIGCE+NEIANEEMNAHLNESNYWHSKLIIDL NNFRNEMESLKN NMELRNEMTELKKKNRQSK QIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM
Sbjct:   53 YLNQNDKFCPGEAKYCQVQKINFTRNVSEDHTANLRISEVIVKCPRQNCETKTELSMMEEHMRTCDKRPISCPFNSIGCEMNEIANEEMNAHLNESNYWHSKLIIDLFNNFRNEMESLKNGNMELRNEMTELKKKNRQSKHQIDNIEFQSTNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEM 275

HSP 2 Score: 61.6178 bits (148), Expect = 6.208e-11
Identity = 46/94 (48.94%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:  341 EMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQSTNQYQKMSEMEAKQLXXXXXXXXXXXXXXXXXXXNESLREEMTELRRENREFKQQID 622
            E+ ++K EN  LR EMTEL+++NR+ KQQIDNI+  STNQ QKM EMEA++ ENA EI              E L EEM  L+ ENR    Q++
Sbjct:  229 EIVAVKKENESLREEMTELRRENREFKQQIDNIQSLSTNQQQKMGEMEAREQENAGEI--------------ELLNEEMVVLKNENRSMSSQME 308
The following BLAST results are available for this feature:
BLAST of TNF receptor associated factor-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
I1ZID82.276e-2336.36TNF receptor associated factor-2 (Fragment) OS=Sch... [more]
A0A0F7GWW38.213e-2036.62TNF receptor associated factor 4 (Fragment) OS=Sch... [more]
T1E1019.196e-1432.17TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
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BLAST of TNF receptor associated factor-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor associated factor-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000049567.11.037e-11188.34SMESG000049567.1[more]
SMESG000049567.11.145e-11188.34SMESG000049567.1[more]
SMESG000049614.11.482e-11087.00SMESG000049614.1[more]
SMESG000049688.12.461e-10986.55SMESG000049688.1[more]
SMESG000049688.13.552e-10986.55SMESG000049688.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025535 ID=SMED30025535|Name=TNF receptor associated factor-2|organism=Schmidtea mediterranea sexual|type=transcript|length=670bp
ATATTTGGATGAAGATGATAAATTCTGTCCAGGAGATTCAAACTTTTGTC
AAGCTAAAATGATTAATTTCACAAAGAATGTAACGGAAGATCCCGCAGTC
AATATAAAGATATCAAAGCGTATTGTGAAATGCCCAAGACAAAAATGTGA
AATCCAAACCGAGCTGATCATGATGGAGGAGCACATGAGAACATGCAAAA
AGCGGTCAATCAGCTGCCCATTCATTTCAATTGGCTGTGAAATAAATGAA
ATAGCCAACGAGGAAATGAATGCACATCTCAATGAGAGCAATTATTGGCA
TAGCAAACTGATTATTGATTTAATCAATAATTTCCGCAATGAGATGGAAT
CACTGAAGAATGAAAATATGGAATTGAGAAATGAAATGACCGAATTGAAG
AAGAAAAACAGACAATCCAAACAACAAATTGATAATATCGAATTTCAGTC
AACAAATCAATATCAGAAAATGAGCGAGATGGAAGCGAAACAGCTGGAAA
ATGCTGAAGAAATAAAGAAAATCAATAAAGAAATTGTAGCCGTAAAGAAG
GAGAATGAATCATTGAGAGAAGAAATGACGGAATTGAGGAGGGAAAACCG
AGAATTCAAGCAACAAATTGACAATATCCAATCGCTATCAACAAATCAAC
AACAGAAAATGGGCGAGATG
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protein sequence of SMED30025535-orf-1

>SMED30025535-orf-1 ID=SMED30025535-orf-1|Name=SMED30025535-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp
YLDEDDKFCPGDSNFCQAKMINFTKNVTEDPAVNIKISKRIVKCPRQKCE
IQTELIMMEEHMRTCKKRSISCPFISIGCEINEIANEEMNAHLNESNYWH
SKLIIDLINNFRNEMESLKNENMELRNEMTELKKKNRQSKQQIDNIEFQS
TNQYQKMSEMEAKQLENAEEIKKINKEIVAVKKENESLREEMTELRRENR
EFKQQIDNIQSLSTNQQQKMGEM
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001293Znf_TRAF
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0004842ubiquitin-protein transferase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006955immune response
GO:0007165signal transduction
GO:0016567protein ubiquitination