SMED30025505
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025505 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SMED30025505 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1) HSP 1 Score: 159.458 bits (402), Expect = 5.465e-65 Identity = 107/264 (40.53%), Postives = 151/264 (57.20%), Query Frame = 3 Query: 900 LNMKRFNSNYLPRILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLSLINKSKLNFKIKDCDNLFYRNGNLCFSIKLSEIKKSDA-TKKIITKSTNFQNKNKTIITNLVNTDNYCSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLLTSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTEGMHKDSDKISSLINHLKLNKEFPKDISFSGCKFI 1688 L +FN R LNK+E+ + ++P +K K S KF ++N +L I SK KI FYRNGN+C SI S K++ KK I K++ I +N++ + N K A AIK+FLFV S KL+ET +I N +G IN TL+ LK Q+ LP +L+KS +KY DGDF+M DLL+ D + + + S L+RSQ+ L+ FPE+ E+ F+E ++DK +LIN+LK FP+ ISF G KFI Sbjct: 4114 LEKTKFNYQCFSRTLNKSEKLQLFRSFPNLNKKIKTKESVEKFQNLNNELIRITPSKNPLKIDGFLKFFYRNGNICVSINFSVNNKTEPLVKKTILKTS-------IIDSNIITSYNNRLKFTGASTAIKRFLFVLSNKLSETATISNAKVRGEQINNTLNLLKKQRALPDYLMKSHIKYHDGDFVMLDLLSDSDLNVGNATLSSSELSRSQMRDLFKSFPELAELFFSES-DCNTDKFKNLINNLKSEHGFPEQISFVGGKFI 4369 HSP 2 Score: 108.997 bits (271), Expect = 5.465e-65 Identity = 55/118 (46.61%), Postives = 73/118 (61.86%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSCQEKLIKYEKWLLNG 2079 GL + ++ D L L S P SS KI++IIFHLNYL LKG +K+ SN +YG YV NY RN L L+SI D E N+L +D +E W L IP SY SC+E+L++ +W L+ Sbjct: 4393 GLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERLLRLRQWKLSS 4510 HSP 3 Score: 140.198 bits (352), Expect = 2.713e-59 Identity = 100/264 (37.88%), Postives = 141/264 (53.41%), Query Frame = 3 Query: 900 LNMKRFNSNYLPRILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLSLINKSKLNFKIKDCDNLFYRNGNLCFSIKLSEIKKSDA-TKKIITKSTNFQNKNKTIITNLVNTDNYCSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLLTSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTEGMHKDSDKISSLINHLKLNKEFPKDISFSGCKFI 1688 L +FN R LNK+E+ + ++P +K K S KF ++N +L I SK KI FYRNGN+C SI S K + KK I K++ I +N++ + N K A AIK+FLFV S KL+ET +I N Q+ LP +L+KS +KY DGDF+M DLL+ D + + + S L+RSQ+ L+ FPE+ E+ F+E ++DK +LIN+LK FP+ ISF G KFI Sbjct: 1780 LEKTKFNYQCFSRTLNKSEKLQLFRSFPNLNKKIKTKESVEKFQNLNNELIRITPSKNPLKIDGFLKFFYRNGNICVSINFSVNNKMEPLVKKTILKTS-------IIDSNIITSYNNRLKFTGASTAIKRFLFVLSNKLSETATISN---------------AKQRALPDYLMKSHIKYHDGDFVMLDLLSDSDLNVGNATLSSSELSRSQMRDLFKSFPELAELFFSES-DCNTDKFKNLINNLKSEHGFPEQISFVGGKFI 2020 HSP 4 Score: 108.997 bits (271), Expect = 2.713e-59 Identity = 55/118 (46.61%), Postives = 73/118 (61.86%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSCQEKLIKYEKWLLNG 2079 GL + ++ D L L S P SS KI++IIFHLNYL LKG +K+ SN +YG YV NY RN L L+SI D E N+L +D +E W L IP SY SC+E+L++ +W L+ Sbjct: 2044 GLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERLLRLRQWKLSS 2161
BLAST of SMED30025505 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1) HSP 1 Score: 159.458 bits (402), Expect = 5.650e-65 Identity = 107/264 (40.53%), Postives = 151/264 (57.20%), Query Frame = 3 Query: 900 LNMKRFNSNYLPRILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLSLINKSKLNFKIKDCDNLFYRNGNLCFSIKLSEIKKSDA-TKKIITKSTNFQNKNKTIITNLVNTDNYCSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLLTSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTEGMHKDSDKISSLINHLKLNKEFPKDISFSGCKFI 1688 L +FN R LNK+E+ + ++P +K K S KF ++N +L I SK KI FYRNGN+C SI S K++ KK I K++ I +N++ + N K A AIK+FLFV S KL+ET +I N +G IN TL+ LK Q+ LP +L+KS +KY DGDF+M DLL+ D + + + S L+RSQ+ L+ FPE+ E+ F+E ++DK +LIN+LK FP+ ISF G KFI Sbjct: 4085 LEKTKFNYQCFSRTLNKSEKLQLFRSFPNLNKKIKTKESVEKFQNLNNELIRITPSKNPLKIDGFLKFFYRNGNICVSINFSVNNKTEPLVKKTILKTS-------IIDSNIITSYNNRLKFTGASTAIKRFLFVLSNKLSETATISNAKVRGEQINNTLNLLKKQRALPDYLMKSHIKYHDGDFVMLDLLSDSDLNVGNATLSSSELSRSQMRDLFKSFPELAELFFSES-DCNTDKFKNLINNLKSEHGFPEQISFVGGKFI 4340 HSP 2 Score: 108.997 bits (271), Expect = 5.650e-65 Identity = 55/118 (46.61%), Postives = 73/118 (61.86%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSCQEKLIKYEKWLLNG 2079 GL + ++ D L L S P SS KI++IIFHLNYL LKG +K+ SN +YG YV NY RN L L+SI D E N+L +D +E W L IP SY SC+E+L++ +W L+ Sbjct: 4364 GLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERLLRLRQWKLSS 4481 HSP 3 Score: 140.198 bits (352), Expect = 2.828e-59 Identity = 100/264 (37.88%), Postives = 141/264 (53.41%), Query Frame = 3 Query: 900 LNMKRFNSNYLPRILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLSLINKSKLNFKIKDCDNLFYRNGNLCFSIKLSEIKKSDA-TKKIITKSTNFQNKNKTIITNLVNTDNYCSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLLTSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTEGMHKDSDKISSLINHLKLNKEFPKDISFSGCKFI 1688 L +FN R LNK+E+ + ++P +K K S KF ++N +L I SK KI FYRNGN+C SI S K + KK I K++ I +N++ + N K A AIK+FLFV S KL+ET +I N Q+ LP +L+KS +KY DGDF+M DLL+ D + + + S L+RSQ+ L+ FPE+ E+ F+E ++DK +LIN+LK FP+ ISF G KFI Sbjct: 1780 LEKTKFNYQCFSRTLNKSEKLQLFRSFPNLNKKIKTKESVEKFQNLNNELIRITPSKNPLKIDGFLKFFYRNGNICVSINFSVNNKMEPLVKKTILKTS-------IIDSNIITSYNNRLKFTGASTAIKRFLFVLSNKLSETATISN---------------AKQRALPDYLMKSHIKYHDGDFVMLDLLSDSDLNVGNATLSSSELSRSQMRDLFKSFPELAELFFSES-DCNTDKFKNLINNLKSEHGFPEQISFVGGKFI 2020 HSP 4 Score: 108.997 bits (271), Expect = 2.828e-59 Identity = 55/118 (46.61%), Postives = 73/118 (61.86%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSCQEKLIKYEKWLLNG 2079 GL + ++ D L L S P SS KI++IIFHLNYL LKG +K+ SN +YG YV NY RN L L+SI D E N+L +D +E W L IP SY SC+E+L++ +W L+ Sbjct: 2044 GLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERLLRLRQWKLSS 2161
BLAST of SMED30025505 vs. Planmine SMEST
Match: SMESG000021487.1 (SMESG000021487.1) HSP 1 Score: 154.451 bits (389), Expect = 1.166e-61 Identity = 118/329 (35.87%), Postives = 180/329 (54.71%), Query Frame = 3 Query: 789 VDELFNDTEKVSNAENSPLN-PINTQFENFKVTVT------KDALNMKRFNS---NYLPRILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLS--LINKSKLNFKIKDC-DNLFYRNGNLCFSIKLSEIKKSDATKKIITKSTNFQNKNKTIITNLVNTDNYCSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLL---TSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTEGMHKDSDKISSLINHLKLNKEFPKDISFSGCKFIIKQKSNKIEPDSW 1727 +D L +++ V+ N+ LN N +NF++T D ++ F + N + RIL KNER + YP I+L K+K S K+ +N LS + LN DC + FYRN N C S L+ KKS K + K+ + KNK +VN + I AP+AIKKFLF S +LAETVSI++D H+G+ INK + ++ +QLP +L+KS++KY DG+F++ DL S D ++ + S L+R+++ LY +P +KE+CF E D + SL+ LK+ FP++I + G KF +K EP+S+ Sbjct: 160 IDNLIDNSNIVNKISNNILNKEFNNGLKNFQITFKNNLIKKTDNFKLQPFENKDFNKVVRILTKNERKTLFIKYPKLINLNKRKESHNKYLELNKILSEYSFQCNPLNL---DCFEYFFYRNQNFCVSFNLNNTKKS--FKPSVLKNNIYLIKNK-----IVNIFPVGASYIPAPMAIKKFLFNVSPELAETVSIRDDKHRGSKINKIISSMIKAQQLPNYLIKSNIKYEDGEFLLLDLTKVKLSSDTTDKLVSSTFSELSRTEMKNLYSSYPSLKEICFVENPSTKHDSVISLVESLKIKNAFPENIFYEGGKFFQSAIKSK-EPNSF 477 HSP 2 Score: 102.834 bits (255), Expect = 1.166e-61 Identity = 52/118 (44.07%), Postives = 76/118 (64.41%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSCQEKLIKYEKWLLNG 2079 GLFDL +SKDD +L+ ++ KIVEIIF+LNYL L G++KY N TY YV NY RN L++S+ E ++ D E W +E+ +IP S++ SCQEK++K ++W ++G Sbjct: 485 GLFDLESMSKDDADLKSILDKQGCDFKIVEIIFYLNYLGLIGSLKYCQNQTYSDYVSNYIRNCCLINSLCSLEDLALVLDDDFEYWATEIIQKIPHESFYYTSCQEKILKLKQWKISG 602
BLAST of SMED30025505 vs. Planmine SMEST
Match: SMESG000068945.1 (SMESG000068945.1) HSP 1 Score: 146.747 bits (369), Expect = 1.691e-50 Identity = 100/120 (83.33%), Postives = 106/120 (88.33%), Query Frame = 3 Query: 501 SMDPEKSFDVCDKKALSNKILPLSAKLMELEEHILRMNSKFDIVLSKLXXXXXXXXXXXXXXXXXXXVDNKDDLNISSNYNLVSENVLNKYIKESSVDELFNDTEKVSNAENSPLNPINT 860 +MDPEK FD CD +ALSNKILPL +KLMELEEHILR KFDIVLSKL NSDFNDNNCNLNI NKIN DN +DLNISSNYN+VSENVLNKY KESSVDELFNDTEKVSN+EN PLN INT Sbjct: 106 AMDPEKLFDECDMQALSNKILPLLSKLMELEEHILR---KFDIVLSKLGNSDFNDNNCNLNIPNKINFDNNEDLNISSNYNIVSENVLNKYNKESSVDELFNDTEKVSNSENGPLNSINT 222 HSP 2 Score: 73.1738 bits (178), Expect = 1.691e-50 Identity = 48/53 (90.57%), Postives = 49/53 (92.45%), Query Frame = 1 Query: 337 ENQIIYSQQLLDFISQRSNGHNQLPLSPKSIHXXXXXXXXXXXXTGDNQLPPS 495 +NQII SQQLLDFISQRSNGHNQLPLS K IH DLSLDLLSKKSTGDNQLPPS Sbjct: 51 KNQIICSQQLLDFISQRSNGHNQLPLSAKPIHPDLSLDLLSKKSTGDNQLPPS 103
BLAST of SMED30025505 vs. Planmine SMEST
Match: SMESG000046455.1 (SMESG000046455.1) HSP 1 Score: 119.013 bits (297), Expect = 1.746e-48 Identity = 101/257 (39.30%), Postives = 147/257 (57.20%), Query Frame = 3 Query: 936 RILNKNERHDIINNYPCFISLLKKKNSKTKFDHINYKLSLINKSKLNFKIKDCDNLFYRNGNLCFSIKLSEIKKSDATKKIITKSTNFQNKNKTIITNLVNTD---NY-CSKLIKAPLAIKKFLFVFSRKLAETVSIKNDLHKGTVINKTLDNLKFQKQLPYFLVKSDLKYADGDFIMKDLLTSEDADNESFNIGLSNLTRSQLNKLYFDFPEIKEMCFTE--GMHKDSDKISSLINHLKLNKEFPKDISFSGCKFI 1688 R+L+ ER +P +LLK K S+ K + +N +L+ I+K I N F+RN N+C +I + S K ITK N I T+L+N+ NY +K I A L+IKKFLFV S KLAET +I N + INK + LK + LP +LVKS + Y DGDF+M DLL S + + E ++ L+RSQ+ +L+ FPEI+E+ F E + ++ +K+ +LIN K+ + FP+ ISF G KF+ Sbjct: 621 RVLDSKERQQFFKLFPNSFALLKSKESEKKSEVLNIELTKISKIDNPLNIAGFKNFFFRNNNICVTI---DFYNSSKVKSKITK-------NLKIKTSLINSKIVKNYNTNKFIPASLSIKKFLFVLSDKLAETTNISNVTVREEQINKIITLLKIKNSLPDYLVKSQINYKDGDFLMLDLLNSFEIEGE-LGQNITELSRSQIKELFNSFPEIQELFFIEKKSIDENPEKLINLINKFKIEETFPQQISFVGGKFV 866 HSP 2 Score: 94.3597 bits (233), Expect = 1.746e-48 Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = 1 Query: 1726 GLFDLFKISKDDIELERLISLPASSNKIVEIIFHLNYLNLKGNVKYFSNITYGTYVLNYFRNVLLLHSIIDSEKCNSLIIDTLEIWGSELTDQIPSTSYFKFSC 2037 GLF LF +S+ D L L+ P S K+V+IIFHLNYLNLKGN +N TY YV NY N LL+HSI+D E C L +D +E+W +L + IPS+S + SC Sbjct: 890 GLFSLFGLSEYDNILNSLLCQPKSPEKLVDIIFHLNYLNLKGNFDTSTNGTYENYVQNYIVNCLLMHSILDLETCAFLNLDNIELWAPKLIELIPSSSDYSSSC 993 The following BLAST results are available for this feature:
BLAST of SMED30025505 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30025505 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30025505 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30025505 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30025505 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025505 ID=SMED30025505|Name=SMED30025505|organism=Schmidtea mediterranea sexual|type=transcript|length=2079bpback to top protein sequence of SMED30025505-orf-1 >SMED30025505-orf-1 ID=SMED30025505-orf-1|Name=SMED30025505-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp MTQSLSVVQAQLASDQLNNSSSELVIFKTILENQIIYSQQLLDFISQRSNback to top protein sequence of SMED30025505-orf-2 >SMED30025505-orf-2 ID=SMED30025505-orf-2|Name=SMED30025505-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=414bp MDPEKSFDVCDKKALSNKILPLSAKLMELEEHILRMNSKFDIVLSKLSNSback to top Annotated Terms
The following terms have been associated with this transcript:
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