mxi-2 protein

Overview


Name	mxi-2 protein (AGZ94931.1)
Smed ID	SMED30025473
Length (bp)	1193

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of mxi-2 protein (SMED30025473) t-SNE clustered cells

Violin plots show distribution of expression levels for mxi-2 protein (SMED30025473) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of mxi-2 protein (SMED30025473) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for mxi-2 protein (SMED30025473) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30025473

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 19

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
gut	SMED30025473	h1SMcG0002887	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
dorsal ventral margin of the whole animal	SMED30025473	h1SMcG0002887	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30025473	h1SMcG0002887	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30025473	h1SMcG0002839	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
dorsal ventral margin of the whole animal	SMED30025473	h1SMcG0002839	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30025473	h1SMcG0002839	Contig1164	GPL15192	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
gut	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
central nervous system	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
progenitor cell	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
stem cell	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
parenchyma	SMED30025473	h1SMcG0002839	KF487116	smed_ncbi_20240424	PMID:24173799 Cowles et al., 2013
epidermis	SMED30025473	h1SMcG0002839	dd_Smed_v4_4048_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of mxi-2 protein vs. Ensembl Human
Match: MXI1 (MAX interactor 1, dimerization protein [Source:HGNC Symbol;Acc:HGNC:7534])

HSP 1 Score: 48.9062 bits (115), Expect = 3.189e-6
Identity = 30/93 (32.26%), Postives = 54/93 (58.06%), Query Frame = 1

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEM 117

[Close]

BLAST of mxi-2 protein vs. Ensembl Zebrafish
Match: mxi1 (max interactor 1, dimerization protein [Source:NCBI gene;Acc:30485])

HSP 1 Score: 50.8322 bits (120), Expect = 8.144e-7
Identity = 37/109 (33.94%), Postives = 61/109 (55.96%), Query Frame = 1

Query:  139 LLWQAAVTLEGSEL-DKKIVEARNKRFHNLKLIKNGCEKTIQN---SNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIR 453
            +L +AA  +E +E  D K        F +++      ++  +N   +NN  +++HNE+E++RRAHLR C+E LK  +    EC  +R T L +L KA + IK  E++ R
Sbjct:   27 VLLEAASYIESAERKDGKCEHGYASTFPSIQHSSYQRQRKFRNKKCNNNHYRSTHNELEKNRRAHLRLCLERLKTLIPLGPEC--SRHTTLGLLNKAKAHIKKLEEADR 133

[Close]

BLAST of mxi-2 protein vs. Ensembl Zebrafish
Match: mxi1 (max interactor 1, dimerization protein [Source:NCBI gene;Acc:30485])

HSP 1 Score: 50.447 bits (119), Expect = 8.536e-7
Identity = 28/62 (45.16%), Postives = 41/62 (66.13%), Query Frame = 1

Query:  268 NNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIR 453
            NN  +++HNE+E++RRAHLR C+E LK  +    EC  +R T L +L KA + IK  E++ R
Sbjct:   53 NNHYRSTHNELEKNRRAHLRLCLERLKTLIPLGPEC--SRHTTLGLLNKAKAHIKKLEEADR 112

[Close]

BLAST of mxi-2 protein vs. Ensembl Zebrafish
Match: mxi1 (max interactor 1, dimerization protein [Source:NCBI gene;Acc:30485])

HSP 1 Score: 50.8322 bits (120), Expect = 9.937e-7
Identity = 28/62 (45.16%), Postives = 41/62 (66.13%), Query Frame = 1

Query:  268 NNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIR 453
            NN  +++HNE+E++RRAHLR C+E LK  +    EC  +R T L +L KA + IK  E++ R
Sbjct:  104 NNHYRSTHNELEKNRRAHLRLCLERLKTLIPLGPEC--SRHTTLGLLNKAKAHIKKLEEADR 163

[Close]

BLAST of mxi-2 protein vs. Ensembl Mouse
Match: Mxi1 (MAX interactor 1, dimerization protein [Source:MGI Symbol;Acc:MGI:97245])

HSP 1 Score: 48.9062 bits (115), Expect = 2.128e-6
Identity = 30/93 (32.26%), Postives = 55/93 (59.14%), Query Frame = 1

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K+RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K+RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K+RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEM 117

[Close]

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K+RL  ++  Q++
Sbjct:   27 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEM 117

[Close]

BLAST of mxi-2 protein vs. Ensembl Mouse
Match: Mxi1 (MAX interactor 1, dimerization protein [Source:MGI Symbol;Acc:MGI:97245])

HSP 1 Score: 49.2914 bits (116), Expect = 4.173e-6
Identity = 30/93 (32.26%), Postives = 55/93 (59.14%), Query Frame = 1

Query:  262 NSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQL 540
            N++   +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R    +   ++ +   +K+RL  ++  Q++
Sbjct:  130 NTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDC--TRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEM 220

[Close]

BLAST of mxi-2 protein vs. UniProt/SwissProt
Match: sp|P50541|MXI1_DANRE (Max-interacting protein 1 OS=Danio rerio OX=7955 GN=mxi1 PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 6.148e-6
Identity = 37/109 (33.94%), Postives = 61/109 (55.96%), Query Frame = 1

Query:  139 LLWQAAVTLEGSEL-DKKIVEARNKRFHNLKLIKNGCEKTIQN---SNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIR 453
            +L +AA  +E +E  D K        F +++      ++  +N   +NN  +++HNE+E++RRAHLR C+E LK  +    EC  +R T L +L KA + IK  E++ R
Sbjct:   27 VLLEAASYIESAERKDGKCEHGYASTFPSIQHSSYQRQRKFRNKKCNNNHYRSTHNELEKNRRAHLRLCLERLKTLIPLGPEC--SRHTTLGLLNKAKAHIKKLEEADR 133

[Close]

BLAST of mxi-2 protein vs. TrEMBL
Match: U5YR79 (Mxi-2 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=mxi-2 PE=2 SV=1)

HSP 1 Score: 387.111 bits (993), Expect = 8.863e-132
Identity = 211/214 (98.60%), Postives = 213/214 (99.53%), Query Frame = 1

Query:  160 TLEGSELDKKIVEARNKRFHNLKLIKNGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSXXXXXXXXXXXXXXXXXXSIIVSKCNSPISTSSDSGFDDLSNSTDHKYPD 801
            TLEGSELDKKIVEARNKRFHNLKLIKNGCEKT+QNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSK KSIRNLYKRECEVKSQNAQ+KQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSKALLINKLNINKIDNQLKSIIVSKCNSPISTSSDSGFDDLSNSTDHKYPD
Sbjct:    1 TLEGSELDKKIVEARNKRFHNLKLIKNGCEKTVQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKGKSIRNLYKRECEVKSQNAQVKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSKALLINKLNINKIDNQLKSIIVSKCNSPISTSSDSGFDDLSNSTDHKYPD 214

[Close]

BLAST of mxi-2 protein vs. TrEMBL
Match: A0A0R3ULQ7 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8625 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 6.081e-18
Identity = 60/125 (48.00%), Postives = 80/125 (64.00%), Query Frame = 1

Query:  286 SHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSK--------YSMYMEKRNRNFSECSINTTSSEESQLD 636
            +HNE+E+SRRAHLR CM+ LK +LVF +E  + RITML +LRKAT+TI+   +  +N Y   CE        KQRL  + K++Q L  K          M   +RNRN+SECS+ TTSSE+S+LD
Sbjct:   73 THNELEKSRRAHLRTCMDALKERLVFDSE--VPRITMLTVLRKATATIQLLRQ--KNEYLEACEDGE-----KQRLSQLLKRRQTLRKKIESKRSRALKMNWRERNRNYSECSVLTTSSEDSELD 188

[Close]

BLAST of mxi-2 protein vs. TrEMBL
Match: A0A0X3P5I7 (Max-interacting protein 1 OS=Schistocephalus solidus OX=70667 GN=MXI1 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.622e-17
Identity = 60/124 (48.39%), Postives = 83/124 (66.94%), Query Frame = 1

Query:  286 SHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEV--KSQNAQIKQRLMYMEKQQQLLCSKYSMYME-----KRNRNFSECSINTTSSEESQLD 636
            +HNE+E+SRRAHLR CME LK +LVF ++  + RITML ILRKA +TI    +  +N Y   CEV  K + AQ+ +R   + K+   L SK + +++     +RNRN+SECS  TTSSE+S+LD
Sbjct:   72 THNELEKSRRAHLRTCMEALKARLVFDSD--VPRITMLTILRKAAATIHILRQ--KNEYLEACEVGEKQRCAQLLKRRQALRKK---LDSKRNRHLKIQSWRERNRNYSECSALTTSSEDSELD 188

[Close]

BLAST of mxi-2 protein vs. TrEMBL
Match: A0A0R3SDA0 (BHLH domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS2643 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 3.399e-16
Identity = 57/128 (44.53%), Postives = 77/128 (60.16%), Query Frame = 1

Query:  280 KNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMY---------MEKRNRNFSECSINTTSSEESQLD 636
            +++HNE+E+SRRAHLR CM+ LK  LVF +E    RIT L +LRKATSTI+  ++  RN Y   CE        K R   + K++Q L  K               +RNRN+SECS+ TTSSE+S++D
Sbjct:   70 RSTHNELEKSRRAHLRTCMDALKELLVFDSEA--PRITTLTVLRKATSTIQQLQQ--RNEYLEACEDGE-----KHRFSQLMKRRQALRKKIEAKRNGTQKIQNWRERNRNYSECSVLTTSSEDSEMD 188

[Close]

BLAST of mxi-2 protein vs. TrEMBL
Match: A0A5J4NUA1 (MAX dimerization protein OS=Paragonimus westermani OX=34504 GN=DEA37_0009825 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.088e-16
Identity = 60/129 (46.51%), Postives = 83/129 (64.34%), Query Frame = 1

Query:  286 SHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKY------SMYME---KRNRNFSECSINTTSSEESQLDDRF 645
            +HNE+E+SRRAHLR CME LK  L F N+  + RITML +L+KAT+TI+S  +  +N Y    E K      KQR   + K++QLL  K       S+ ++   +RNRN SECSINTTSS++S++D + 
Sbjct:   70 THNELEKSRRAHLRACMEALKEHLNFDND--VPRITMLTVLKKATATIQSLRQ--KNQYLETYEDKE-----KQRCAQLIKRRQLLKKKLEEKRNRSLKLQNWRERNRNSSECSINTTSSDDSEMDQQL 189

[Close]

BLAST of mxi-2 protein vs. Ensembl Cavefish
Match: mxi1 (MAX interactor 1, dimerization protein [Source:NCBI gene;Acc:103033352])

HSP 1 Score: 50.447 bits (119), Expect = 1.019e-6
Identity = 29/65 (44.62%), Postives = 44/65 (67.69%), Query Frame = 1

Query:  280 KNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIR-NLYKRE 471
            +++HNE+E++RRAHLR C+E LK  +    +C  NR T L +L KA + IK  E++ R +LY+ E
Sbjct:  111 RSTHNELEKNRRAHLRLCLERLKALIPLGPDC--NRHTTLGLLNKAKAHIKKLEEADRKSLYQLE 173

[Close]

BLAST of mxi-2 protein vs. Ensembl Sea Lamprey
Match: mxd1 (MAX dimerization protein 1 [Source:ZFIN;Acc:ZDB-GENE-060526-246])

HSP 1 Score: 47.7506 bits (112), Expect = 1.208e-6
Identity = 28/77 (36.36%), Postives = 49/77 (63.64%), Query Frame = 1

Query:  283 NSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRL 513
            ++HNE+E++RRAHLR C+E LK  +    EC  +R T L +L KA + IK  E+  R    ++ +++ ++  +++RL
Sbjct:   69 STHNELEKNRRAHLRKCLERLKEMVPLEAEC--SRHTTLGLLNKAKNHIKRLEEQDRRAQHQKDQLEREHRYLRRRL 143

[Close]

BLAST of mxi-2 protein vs. Ensembl Medaka
Match: mxi1 (max-interacting protein 1 [Source:NCBI gene;Acc:101164381])

HSP 1 Score: 50.8322 bits (120), Expect = 8.244e-7
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 1

Query:  181 DKKIVEARNKRFHNLKLIKNGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLL 543
            D+     R +RF N K            +NN  +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R         KSQN     +L  +E++Q+ L
Sbjct:   89 DQSTSYQRTRRFRNKKF-----------NNNHNRSTHNELEKNRRAHLRLCLERLKALIPLGPDC--TRHTTLGLLTKAKAHIKKLEEADR---------KSQN-----QLQSLEREQRHL 182

[Close]

BLAST of mxi-2 protein vs. Ensembl Medaka
Match: mxi1 (max-interacting protein 1 [Source:NCBI gene;Acc:101164381])

HSP 1 Score: 50.0618 bits (118), Expect = 8.848e-7
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 1

Query:  181 DKKIVEARNKRFHNLKLIKNGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLL 543
            D+     R +RF N K            +NN  +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R         KSQN     +L  +E++Q+ L
Sbjct:   37 DQSTSYQRTRRFRNKKF-----------NNNHNRSTHNELEKNRRAHLRLCLERLKALIPLGPDC--TRHTTLGLLTKAKAHIKKLEEADR---------KSQN-----QLQSLEREQRHL 130

[Close]

BLAST of mxi-2 protein vs. Ensembl Medaka
Match: mxi1 (max-interacting protein 1 [Source:NCBI gene;Acc:101164381])

HSP 1 Score: 50.0618 bits (118), Expect = 1.025e-6
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 1

Query:  181 DKKIVEARNKRFHNLKLIKNGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLL 543
            D+     R +RF N K            +NN  +++HNE+E++RRAHLR C+E LK  +    +C   R T L +L KA + IK  E++ R         KSQN     +L  +E++Q+ L
Sbjct:   37 DQSTSYQRTRRFRNKKF-----------NNNHNRSTHNELEKNRRAHLRLCLERLKALIPLGPDC--TRHTTLGLLTKAKAHIKKLEEADR---------KSQN-----QLQSLEREQRHL 130

[Close]

BLAST of mxi-2 protein vs. Ensembl Medaka
Match: mxd3 (MAX dimerization protein 3 [Source:NCBI gene;Acc:101174840])

HSP 1 Score: 47.7506 bits (112), Expect = 5.416e-6
Identity = 42/129 (32.56%), Postives = 69/129 (53.49%), Query Frame = 1

Query:  250 KTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLD 636
            K I    N  ++ HNE+E++RRA LR+C+E+LK+++  S++   +R T L +LR+A   IK  +           E   +  Q+K RL++ +++ ++   +     E R RN S  S  T SSE S  D
Sbjct:   49 KKISVGGNSNRSVHNELEKNRRAQLRHCLEQLKKQVPLSSDS--SRNTTLSLLRQAQLHIKKLQ-----------EQDERAEQLKGRLLWEQRELRIRLEQLERGTE-RMRNNSHGS--TMSSERSDSD 161

[Close]

BLAST of mxi-2 protein vs. Planmine SMEST
Match: SMESG000060394.1 (SMESG000060394.1)

HSP 1 Score: 421.394 bits (1082), Expect = 1.385e-148
Identity = 227/229 (99.13%), Postives = 228/229 (99.56%), Query Frame = 1

Query:  115 MIQSDRLLLLWQAAVTLEGSELDKKIVEARNKRFHNLKLIKNGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSXXXXXXXXXXXXXXXXXXSIIVSKCNSPISTSSDSGFDDLSNSTDHKYPD 801
            MIQSDRLLLLWQAAVTLEGSELDKKIVEARNKRFHNLKLIKNGCEKT+QNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSKALLINKLNINKIDNQLKSIIVSKCNSPISTSSDSGFDDLSNS DHKYPD
Sbjct:    1 MIQSDRLLLLWQAAVTLEGSELDKKIVEARNKRFHNLKLIKNGCEKTVQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYMEKRNRNFSECSINTTSSEESQLDDRFHSKALLINKLNINKIDNQLKSIIVSKCNSPISTSSDSGFDDLSNSADHKYPD 229

[Close]

BLAST of mxi-2 protein vs. Planmine SMEST
Match: SMESG000047681.1 (SMESG000047681.1)

HSP 1 Score: 140.198 bits (352), Expect = 2.357e-38
Identity = 96/252 (38.10%), Postives = 145/252 (57.54%), Query Frame = 1

Query:  115 MIQSDRLLLLWQAAVTLEGSELDKKIVEARNKRFHNLKLIK-------NGCEKTIQNSNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRKATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMYME----KRNRNFSECSINTTSSEESQLDD------------------RFHSXXXXXXXXXXXXXXXXXXSII-----VSKCNSPISTSSDSGFDD 768
            M  ++ LLLLW AAV +EGSE+++ I++ARN+R H LKL+K       NG +  I      I++SHNE+E+ RRAHLR+CME+LK+ L F  +    RITML++L+KA+STI ++E++I ++  +E   K++  ++  RL  +++Q + L +  +M ME    +R RN+SECS+ TTSS++S  D                     H+              NQ  S+      V KCNSP S S DSGF++
Sbjct:    1 MADNNGLLLLWNAAVEIEGSEIEQMILQARNRRNHTLKLLKSQDLSFHNGKKSQIP-----IRHSHNELEKCRRAHLRSCMEKLKKVLRFDQDSVPCRITMLNVLKKASSTIYTRERNIHSMNMKETMAKNRFVELTNRLNNLQRQYKRLKNMEAMSMESTKAERMRNYSECSVLTTSSDDSNCDGVRSRRTSTNYSKFYDQTSPVHNYKDKELSKYYKMSTNQDDSLSNSGNNVYKCNSPNSNSCDSGFEE 247

The following BLAST results are available for this feature:

BLAST of mxi-2 protein vs. Ensembl Human
BLAST of mxi-2 protein vs. Ensembl Celegans
BLAST of mxi-2 protein vs. Ensembl Fly
BLAST of mxi-2 protein vs. Ensembl Zebrafish
BLAST of mxi-2 protein vs. Ensembl Xenopus
BLAST of mxi-2 protein vs. Ensembl Mouse
BLAST of mxi-2 protein vs. UniProt/SwissProt
BLAST of mxi-2 protein vs. TrEMBL
BLAST of mxi-2 protein vs. Ensembl Cavefish
BLAST of mxi-2 protein vs. Ensembl Sea Lamprey
BLAST of mxi-2 protein vs. Ensembl Yeast
BLAST of mxi-2 protein vs. Ensembl Nematostella
BLAST of mxi-2 protein vs. Ensembl Medaka
BLAST of mxi-2 protein vs. Planmine SMEST

BLAST of mxi-2 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MXI1	3.189e-6	32.26	MAX interactor 1, dimerization protein [Source:HGN...	[more]
MXI1	3.189e-6	32.26	MAX interactor 1, dimerization protein [Source:HGN...	[more]
MXI1	3.189e-6	32.26	MAX interactor 1, dimerization protein [Source:HGN...	[more]
MXI1	3.189e-6	32.26	MAX interactor 1, dimerization protein [Source:HGN...	[more]
MXI1	3.189e-6	32.26	MAX interactor 1, dimerization protein [Source:HGN...	[more]

BLAST of mxi-2 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of mxi-2 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of mxi-2 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
mxi1	8.144e-7	33.94	max interactor 1, dimerization protein [Source:NCB...	[more]
mxi1	8.536e-7	45.16	max interactor 1, dimerization protein [Source:NCB...	[more]
mxi1	9.937e-7	45.16	max interactor 1, dimerization protein [Source:NCB...	[more]

BLAST of mxi-2 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of mxi-2 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Mxi1	2.128e-6	32.26	MAX interactor 1, dimerization protein [Source:MGI...	[more]
Mxi1	2.128e-6	32.26	MAX interactor 1, dimerization protein [Source:MGI...	[more]
Mxi1	2.128e-6	32.26	MAX interactor 1, dimerization protein [Source:MGI...	[more]
Mxi1	2.128e-6	32.26	MAX interactor 1, dimerization protein [Source:MGI...	[more]
Mxi1	4.173e-6	32.26	MAX interactor 1, dimerization protein [Source:MGI...	[more]

BLAST of mxi-2 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1


Match Name	E-value	Identity	Description
sp\|P50541\|MXI1_DANRE	6.148e-6	33.94	Max-interacting protein 1 OS=Danio rerio OX=7955 G...	[more]

BLAST of mxi-2 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
U5YR79	8.863e-132	98.60	Mxi-2 protein (Fragment) OS=Schmidtea mediterranea...	[more]
A0A0R3ULQ7	6.081e-18	48.00	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]
A0A0X3P5I7	6.622e-17	48.39	Max-interacting protein 1 OS=Schistocephalus solid...	[more]
A0A0R3SDA0	3.399e-16	44.53	BHLH domain-containing protein OS=Hymenolepis dimi...	[more]
A0A5J4NUA1	6.088e-16	46.51	MAX dimerization protein OS=Paragonimus westermani...	[more]

BLAST of mxi-2 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mxi1	1.019e-6	44.62	MAX interactor 1, dimerization protein [Source:NCB...	[more]

BLAST of mxi-2 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mxd1	1.208e-6	36.36	MAX dimerization protein 1 [Source:ZFIN;Acc:ZDB-GE...	[more]

BLAST of mxi-2 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of mxi-2 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of mxi-2 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4


Match Name	E-value	Identity	Description
mxi1	8.244e-7	33.88	max-interacting protein 1 [Source:NCBI gene;Acc:10...	[more]
mxi1	8.848e-7	33.88	max-interacting protein 1 [Source:NCBI gene;Acc:10...	[more]
mxi1	1.025e-6	33.88	max-interacting protein 1 [Source:NCBI gene;Acc:10...	[more]
mxd3	5.416e-6	32.56	MAX dimerization protein 3 [Source:NCBI gene;Acc:1...	[more]

BLAST of mxi-2 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000060394.1	1.385e-148	99.13	SMESG000060394.1	[more]
SMESG000047681.1	2.357e-38	38.10	SMESG000047681.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30025473
GenBank	AGZ94931.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30025473 ID=SMED30025473|Name=mxi-2 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1193bp
TTGAAACTTAATTCTATTCGCTGGTGATTAACCAATCAAAATTAAGAATT
TTTATAGTTAAATACAGTAAATAAATAGGTCAGGAAAATGAAAAACAAAT
TTTATTAGAAAAAAATGATTCAAAGTGATCGACTACTTTTGTTATGGCAA
GCTGCAGTTACCCTGGAAGGATCAGAATTGGACAAAAAGATTGTGGAAGC
TAGAAATAAAAGATTTCACAATTTAAAATTGATTAAAAATGGTTGTGAGA
AAACTATTCAAAATTCGAATAATGTGATAAAAAATTCGCACAATGAAGTT
GAAAGATCAAGAAGAGCTCATTTACGAAATTGCATGGAAGAATTGAAAAG
AAAATTGGTTTTTAGCAACGAATGCCATTTAAATCGAATTACAATGCTCC
ACATTCTCCGAAAAGCCACATCGACAATTAAATCGAAAGAAAAATCTATT
CGAAACTTATACAAGCGAGAATGTGAAGTTAAAAGTCAAAATGCTCAAAT
AAAACAACGCCTTATGTATATGGAAAAACAACAGCAACTTTTGTGTAGTA
AATACTCAATGTATATGGAGAAGCGAAATCGAAACTTTTCCGAATGTTCT
ATAAACACGACCTCGTCTGAAGAATCGCAATTAGATGATCGGTTTCACTC
AAAAGCACTTCTGATCAATAAATTAAACATAAATAAAATTGATAATCAAC
TAAAATCTATCATAGTTTCCAAATGTAATTCACCGATCTCGACAAGCTCA
GATTCCGGATTCGATGATTTATCAAATAGTACTGACCACAAGTATCCTGA
CTCGATATCCTCACGACCAATATCATTATTATCCTCATTGTCCCAATTGA
AAAAATCAAATGTGTTCTAATTTTGTACAGTATTGAATGTGTTTTTTTCA
ATCTGTAGTGTAATTTAATATTAATGTTTTTCTGCCAACCGATCGGGACC
AGAAAATAGTGTCAGTGTTAACAATAATTTTACTGAAAATTGACTCAGTT
TCATATGTTGAGAAAAAAAAGATATTAATGACATCTATTTACCTTTTTCC
TTGTGTTGGCCTTATTGTGCAGCTATTTGGTCAAAACAAAATCCGACTTT
CGAGTTTTTTGGGGTAAAAACAATTGTATTTCGCATAATCAGATATAGTT
TGAAATTTTATTTTATTATGTTGTAATATTATTATTAGTATTG

protein sequence of SMED30025473-orf-1

>SMED30025473-orf-1 ID=SMED30025473-orf-1|Name=SMED30025473-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=252bp
MIQSDRLLLLWQAAVTLEGSELDKKIVEARNKRFHNLKLIKNGCEKTIQN
SNNVIKNSHNEVERSRRAHLRNCMEELKRKLVFSNECHLNRITMLHILRK
ATSTIKSKEKSIRNLYKRECEVKSQNAQIKQRLMYMEKQQQLLCSKYSMY
MEKRNRNFSECSINTTSSEESQLDDRFHSKALLINKLNINKIDNQLKSII
VSKCNSPISTSSDSGFDDLSNSTDHKYPDSISSRPISLLSSLSQLKKSNV
F*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000026	gut
PLANA:0000034	epidermis
PLANA:0000043	dorsal ventral margin of the whole animal
PLANA:0000070	intestinal phagocyte
PLANA:0000099	neuron
PLANA:0000103	central nervous system
PLANA:0000120	progenitor cell
PLANA:0000121	stem cell
PLANA:0002032	epidermal cell
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR036638	HLH_DNA-bd_sf
IPR011598	bHLH_dom

Vocabulary: **molecular function**
Term	Definition
GO:0046983	protein dimerization activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	G3DSA:4.10.280.10		coord: 56..136 e-value: 4.2E-11 score: 44.8
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 53..124
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 56..107 e-value: 1.7E-6 score: 27.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 54..108 score: 11.407
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	CDD	cd00083	HLH	coord: 56..113 e-value: 9.42336E-5 score: 37.5787

Main menu

mxi-2 protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 19

Owlery

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